JP4123275B2 - 制御構造、分離装置およびグラディエント形成装置ならびにそれらを用いるマイクロチップ - Google Patents
制御構造、分離装置およびグラディエント形成装置ならびにそれらを用いるマイクロチップ Download PDFInfo
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- JP4123275B2 JP4123275B2 JP2005517672A JP2005517672A JP4123275B2 JP 4123275 B2 JP4123275 B2 JP 4123275B2 JP 2005517672 A JP2005517672 A JP 2005517672A JP 2005517672 A JP2005517672 A JP 2005517672A JP 4123275 B2 JP4123275 B2 JP 4123275B2
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- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/06—Valves, specific forms thereof
- B01L2400/0688—Valves, specific forms thereof surface tension valves, capillary stop, capillary break
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/08—Regulating or influencing the flow resistance
- B01L2400/084—Passive control of flow resistance
- B01L2400/086—Passive control of flow resistance using baffles or other fixed flow obstructions
-
- F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
- F16—ENGINEERING ELEMENTS AND UNITS; GENERAL MEASURES FOR PRODUCING AND MAINTAINING EFFECTIVE FUNCTIONING OF MACHINES OR INSTALLATIONS; THERMAL INSULATION IN GENERAL
- F16K—VALVES; TAPS; COCKS; ACTUATING-FLOATS; DEVICES FOR VENTING OR AERATING
- F16K99/00—Subject matter not provided for in other groups of this subclass
- F16K2099/0073—Fabrication methods specifically adapted for microvalves
- F16K2099/0074—Fabrication methods specifically adapted for microvalves using photolithography, e.g. etching
-
- F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
- F16—ENGINEERING ELEMENTS AND UNITS; GENERAL MEASURES FOR PRODUCING AND MAINTAINING EFFECTIVE FUNCTIONING OF MACHINES OR INSTALLATIONS; THERMAL INSULATION IN GENERAL
- F16K—VALVES; TAPS; COCKS; ACTUATING-FLOATS; DEVICES FOR VENTING OR AERATING
- F16K99/00—Subject matter not provided for in other groups of this subclass
- F16K2099/0082—Microvalves adapted for a particular use
- F16K2099/0084—Chemistry or biology, e.g. "lab-on-a-chip" technology
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/40—Concentrating samples
- G01N1/4005—Concentrating samples by transferring a selected component through a membrane
- G01N2001/4016—Concentrating samples by transferring a selected component through a membrane being a selective membrane, e.g. dialysis or osmosis
-
- G—PHYSICS
- G01—MEASURING; TESTING
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- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
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- G01N30/26—Conditioning of the fluid carrier; Flow patterns
- G01N30/28—Control of physical parameters of the fluid carrier
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- G01N2030/347—Control of physical parameters of the fluid carrier of fluid composition, e.g. gradient mixers
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
- G01N35/1095—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices for supplying the samples to flow-through analysers
- G01N35/1097—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices for supplying the samples to flow-through analysers characterised by the valves
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Description
本発明による装置は、上記の制御構造を備える装置である。
(実施形態1)
図1は、本実施形態の制御構造の構成を示す平面図である。
(実施形態2)
図7は、本実施形態の制御構造の構成を示す部分断面図である。
さらに、この疎水性処理はスタンプやインクジェットなどの印刷技術を用いて行うこともできる。
(実施形態3)
図16(a),(b)は、本実施形態の制御構造の構成を示す部分断面図である。
図17は、本実施形態の制御構造の要部の一例を示す部分平面図である。
(実施形態4)
図2は、本実施形態の制御構造の構成を示す平面図である。
(実施形態5)
図3は、本実施形態の制御構造の要部を示す平面図である。
図4(a),(b)は、それぞれ本実施形態の制御構造の構成を別の角度から示す図である。
図8は、本実施形態の制御構造の製造途中の断面図である。
(実施形態6)
図18は、本発明の一実施形態の制御構造の構成を示す部分概略図である。
(実施形態7)
図26は、本発明の一実施形態の制御構造の構成を示す概略図である。
(実施形態8)
図9(A),(B)は、本実施形態の制御構造を備える装置を示す図である。
本実施形態の装置は、複数の流路と、上記の制御構造と、を備える装置である。
(a)リガンドとレセプター
(b)抗原と抗体
(c)酵素と基質、酵素と基質誘導体、または酵素と阻害剤
(d)糖とレクチン
(e)DNA(デオキシリボ核酸)とRNA(リボ核酸)、またはDNAとDNA
(f)タンパク質と核酸
(g)金属とタンパク質
(実施形態9)
(実施形態10)
次に、この制御構造を備える装置を利用した特定物質の分離方法について図9を参照しながら説明する。
(実施形態11)
図11は、本実施形態のグラディエント形成装置を示す概略図である。
図14は、本実施形態の図12に示した隔壁を有するグラディエント形成装置によるグラディエント形成の様子を示す概念図である。
図20は、本実施形態のグラディエント形成装置の断面図である。
図21は、本実施形態のグラディエント形成装置の平面図である。
図24は、本実施形態のグラディエント形成装置の順流路および逆流路の構成の一例を示す図である。
また、図25に示したように、順流路および逆流路をスパイラル状に設けてもよい。
(実施形態12)
図22は、本実施形態のグラディエント形成装置の隔壁の構成を示す概略図である。
(実施形態13)
図27は、本実施形態のグラディエント形成装置の隔壁の構成を示す概略図である。
(実施形態14)
以下、本実施形態のグラディエント形成方法について、図11の実施形態11のグラディエント形成装置の説明を参照して説明する。
(実施形態15)
図15は、本実施形態のマイクロチップの一例としてのアフィニティークロマトグラフィー装置を示す概略図である。
(実施形態16)
(実施形態17)
(実施形態18)
(実施形態19)
Claims (18)
- 第一の液体の通る第一の流路と、
前記第一の流路に連通し、前記第一の液体を堰き止める堰き止め部と、
第二の液体を前記堰き止め部に導く第二の流路と、
を備え、
前記第一の流路から前記第二の流路への前記第一の液体の通過を制御することを特徴とする制御構造。 - 第一の流路と、
第二の流路と、
これらの流路に連通する連通部と、
前記連通部に設けられ、第一の流路から第二の流路への第一の液体の流動を堰き止める堰き止め部と、を備え、
前記堰き止め部は;
第二の流路に液体が存在しないとき、前記第一の流路から前記第二の流路への前記第一の液体の通過を制限し、
第二の流路に液体が存在するとき、前記第一の流路と前記第二の流路との間の液体の流通を許容する
ことを特徴とする制御構造。 - 請求の範囲第1項または第2項に記載の制御構造において、
前記第一の流路と、前記第二の流路とは、前記堰き止め部の近傍の領域で互いに並行することを特徴とする制御構造。 - 請求の範囲第1項乃至第3項いずれかに記載の制御構造において、
前記第一の流路および前記第二の流路は、単一の基板上に形成されている流路溝であることを特徴とする制御構造。 - 請求の範囲第1項乃至第4項いずれかに記載の制御構造において、
前記堰き止め部は、前記第一の液体に対する疎液性が前記第一の流路よりも高い領域を備えることを特徴とする制御構造。 - 請求の範囲第1項乃至第5項いずれかに記載の制御構造において、
前記堰き止め部は、前記第一の流路の単位体積あたり表面積よりも大きい単位体積あたり表面積を有することを特徴とする制御構造。 - 請求の範囲第1項乃至第6項いずれかに記載の制御構造において、
前記堰き止め部は、前記第一の流路と前記第二の流路とを隔てる隔壁に設けられた複数の連通流路からなることを特徴とする制御構造。 - 請求の範囲第1項乃至第7項いずれかに記載の制御構造において、
前記堰き止め部は、多孔質体を備えることを特徴とする制御構造。 - 請求の範囲第1項乃至第8項いずれかに記載の制御構造において、
前記堰き止め部は、単数または複数の突起部を備えることを特徴とする制御構造。 - 請求の範囲第1項乃至第9項いずれかに記載の制御構造において、
前記第一の流路は、外部雰囲気と連通する第一の開口部を備え、
前記第二の流路は、外部雰囲気と連通する第二の開口部を備えることを特徴とする制御構造。 - 試料液中の特定物質を分離する分離部と、
請求の範囲第1項乃至第10項いずれかに記載の制御構造と、
前記試料液の導入部と、
洗浄液の導入部と、
前記特定物質の脱離液の導入部と、
を備え、
前記制御構造は、前記分離部と前記第一の流路を介して連通し、
前記試料液の導入部および前記洗浄液の導入部は、前記第一の流路に、前記制御構造と前記分離部との間で連通し、
前記脱離液の導入部は、前記制御構造に前記第二の流路を介して連通することを特徴とする分離装置。 - 第一の組成液が流れる順流路と、
前記順流路と並行し、第二の組成液が流れる逆流路と、
前記順流路に連通し、前記第一の組成液の原液を前記順流路に導入する第一の導入部と、
前記順流路の下流側において前記逆流路に連通し、前記第二の組成液の原液を前記逆流路に導入する第二の導入部と、
前記順流路と前記逆流路とを隔て、前記第一の組成液または前記第二の組成液の少なくとも特定成分が通過可能な隔壁と、
を備えることを特徴とするグラディエント形成装置。 - 請求の範囲第12項に記載のグラディエント形成装置において、
前記順流路および前記逆流路は、単一の基板上に形成されている流路溝であることを特徴とするグラディエント形成装置。 - 請求の範囲第12項または第13項に記載のグラディエント形成装置において、
前記隔壁は、前記順流路および前記逆流路に連通する複数の流路を備えることを特徴とするグラディエント形成装置。 - 請求の範囲第12項または第13項に記載のグラディエント形成装置において、
前記隔壁は、少なくとも前記特定成分を透過させる膜からなることを特徴とするグラディエント形成装置。 - 請求の範囲第12項乃至第15項いずれかに記載のグラディエント形成装置において、
前記逆流路の前記隔壁と接する領域の下流側に設けられた、前記第二の組成液を堰き止める堰き止め部と、前記堰き止め部またはその下流側の箇所で前逆流路に連通し、前記第一の導入部またはその下流側の箇所で前記順流路と連通し、前記堰き止め部へ前記第一の組成液を導くトリガー流路と、を備える液体スイッチをさらに備えることを特徴とするグラディエント形成装置。 - 基板と、前記基板上に形成されている請求の範囲第11項に記載の分離装置と、前記基板上に形成されているグラディエント形成装置と、を備え、
前記グラディエント形成装置は;
第一の組成液が流れる順流路と、
前記順流路と並行し、第二の組成液が流れる逆流路と、
前記順流路に連通し、前記第一の組成液の原液を前記順流路に導入する第一の導入部と、
前記順流路の下流側において前記逆流路に連通し、前記第二の組成液の原液を前記逆流路に導入する第二の導入部と、
前記順流路と前記逆流路とを隔て、前記第一の組成液または前記第二の組成液の少なくとも特定成分が通過可能な隔壁と、
を備え、
前記グラディエント液採取部は;
前記分離装置に含まれる前記脱離液の導入部と連通することを特徴とするマイクロチップ。 - 生体試料を分子サイズまたは性状に応じて分離する分離手段と、前記分離手段により分離された試料に対し、酵素消化処理を含む前処理を行う前処理手段と、前処理された試料を乾燥させる乾燥手段と、乾燥後の試料を質量分析する質量分析手段と、を備え、
前記分離手段は、請求の範囲第17項に記載のマイクロチップを含むことを特徴とする質量分析システム。
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PCT/JP2005/001381 WO2005075975A1 (ja) | 2004-02-06 | 2005-02-01 | 制御構造、分離装置およびグラディエント形成装置ならびにそれらを用いるマイクロチップ |
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US20060252087A1 (en) * | 2005-01-18 | 2006-11-09 | Biocept, Inc. | Recovery of rare cells using a microchannel apparatus with patterned posts |
KR20070116585A (ko) | 2005-01-18 | 2007-12-10 | 바이오셉트 인코포레이티드 | 패턴화된 포스트를 갖는 마이크로채널을 이용하는 세포분리법 |
JP4596428B2 (ja) * | 2005-06-17 | 2010-12-08 | 株式会社エンプラス | 流体取扱装置 |
SE0700930L (sv) * | 2007-04-16 | 2008-01-22 | Aamic Ab | Analysanordning för vätskeformiga prov |
EP2230504B1 (en) | 2008-01-08 | 2013-04-24 | Nippon Telegraph and Telephone Corporation | Capillary pump unit and flow cell |
EP2103352A1 (en) * | 2008-03-18 | 2009-09-23 | Koninklijke Philips Electronics N.V. | Membranes suited for immobilizing biomolecules |
JP5112371B2 (ja) * | 2009-03-27 | 2013-01-09 | 株式会社東芝 | 気流発生装置 |
NL2002862C2 (en) * | 2009-05-08 | 2010-11-09 | Friesland Brands Bv | Microfluidic apparatus and method for generating a dispersion. |
CN101966473B (zh) * | 2010-10-26 | 2012-05-02 | 武汉大学 | 基于超声驻波的微流控筛选芯片及其制备方法 |
EP2931427B1 (en) * | 2012-12-13 | 2022-02-09 | Siemens Healthineers Nederland B.V. | Fluidic system with fluidic stop |
GB201320146D0 (en) * | 2013-11-14 | 2014-01-01 | Cambridge Entpr Ltd | Fluidic separation and detection |
US10118124B2 (en) * | 2016-01-25 | 2018-11-06 | Ut-Battelle, Llc | Integrated membrane-pyrolysis systems and methods |
US11097270B2 (en) | 2016-07-15 | 2021-08-24 | Hewlett-Packard Development Company, L.P. | Microfluidic filtering system |
CA3034556A1 (en) * | 2016-09-15 | 2018-03-22 | Softhale Nv | Valve, in particular for a device for administering a liquid medicament, and a corresponding device for administering a liquid medicament |
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WO1997047390A1 (en) * | 1996-06-14 | 1997-12-18 | University Of Washington | Absorption-enhanced differential extraction device |
FR2790684B1 (fr) * | 1999-03-09 | 2001-05-11 | Biomerieux Sa | Appareil permettant en son sein le transfert de liquides par capillarite |
JP3780917B2 (ja) * | 2001-11-22 | 2006-05-31 | 株式会社島津製作所 | 液体クロマトグラフとその溶離液混合装置 |
JP4083452B2 (ja) * | 2002-03-28 | 2008-04-30 | 旭化成株式会社 | バルブ機構 |
JP4432778B2 (ja) * | 2002-11-29 | 2010-03-17 | 日本電気株式会社 | マイクロチップおよび質量分析システム |
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