JP4094674B2 - ペプチド特異的т細胞のアッセイ方法 - Google Patents
ペプチド特異的т細胞のアッセイ方法 Download PDFInfo
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- JP4094674B2 JP4094674B2 JP52441098A JP52441098A JP4094674B2 JP 4094674 B2 JP4094674 B2 JP 4094674B2 JP 52441098 A JP52441098 A JP 52441098A JP 52441098 A JP52441098 A JP 52441098A JP 4094674 B2 JP4094674 B2 JP 4094674B2
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- cells
- peptide
- assay
- cytokine
- cell
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- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
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Images
Classifications
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
- G01N33/56966—Animal cells
- G01N33/56972—White blood cells
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/35—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Mycobacteriaceae (F)
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- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
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- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
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- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
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- G01N33/56983—Viruses
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- G01N33/6863—Cytokines, i.e. immune system proteins modifying a biological response such as cell growth proliferation or differentiation, e.g. TNF, CNF, GM-CSF, lymphotoxin, MIF or their receptors
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- G01N33/6878—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids in epitope analysis
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Chemical & Material Sciences (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Biomedical Technology (AREA)
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- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
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- Analytical Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Virology (AREA)
- Microbiology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Gastroenterology & Hepatology (AREA)
- Genetics & Genomics (AREA)
- AIDS & HIV (AREA)
- Biophysics (AREA)
- Peptides Or Proteins (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Analysing Materials By The Use Of Radiation (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB9624456.1 | 1996-11-25 | ||
| GBGB9624456.1A GB9624456D0 (en) | 1996-11-25 | 1996-11-25 | Assay method |
| PCT/GB1997/003222 WO1998023960A1 (en) | 1996-11-25 | 1997-11-25 | Assay method for peptide specific t-cells |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2001505568A JP2001505568A (ja) | 2001-04-24 |
| JP2001505568A5 JP2001505568A5 (enExample) | 2005-07-14 |
| JP4094674B2 true JP4094674B2 (ja) | 2008-06-04 |
Family
ID=10803428
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP52441098A Expired - Lifetime JP4094674B2 (ja) | 1996-11-25 | 1997-11-25 | ペプチド特異的т細胞のアッセイ方法 |
Country Status (11)
| Country | Link |
|---|---|
| US (3) | US7575870B1 (enExample) |
| EP (2) | EP0941478B2 (enExample) |
| JP (1) | JP4094674B2 (enExample) |
| AT (2) | ATE368052T1 (enExample) |
| AU (1) | AU728357C (enExample) |
| CA (1) | CA2272881C (enExample) |
| DE (2) | DE69710360T3 (enExample) |
| DK (2) | DK0941478T4 (enExample) |
| ES (1) | ES2172773T5 (enExample) |
| GB (1) | GB9624456D0 (enExample) |
| WO (1) | WO1998023960A1 (enExample) |
Families Citing this family (41)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB9624456D0 (en) | 1996-11-25 | 1997-01-15 | Isis Innovation | Assay method |
| CA2348475A1 (en) | 1998-11-04 | 2000-05-11 | Isis Innovation Limited | Tuberculosis diagnostic test |
| US20020131976A1 (en) | 1998-12-23 | 2002-09-19 | Ajit Lalvani | Tuberculosis vaccine |
| GB9923306D0 (en) | 1999-10-01 | 1999-12-08 | Isis Innovation | Diagnostic and therapeutic epitope, and transgenic plant |
| GB0006692D0 (en) * | 2000-03-20 | 2000-05-10 | Glaxo Group Ltd | Epitopes |
| US20030198941A1 (en) * | 2000-04-12 | 2003-10-23 | Watkins David I. | Method for making an HIV vaccine |
| WO2002054072A2 (en) | 2001-01-08 | 2002-07-11 | Isis Innovation Limited | Assay to determine efficacy of treatment for mycobacterial infection |
| DE10125730A1 (de) * | 2001-05-17 | 2002-11-21 | A I D Autoimmun Diagnostika Gm | Verfahren zum Nachweis von Erregern und durch diese induzierten zellulär produzierten Stoffen im lebenden Organismus |
| GB0212885D0 (en) | 2002-06-05 | 2002-07-17 | Isis Innovation | Therapeutic epitopes and uses thereof |
| US7153659B2 (en) * | 2003-09-05 | 2006-12-26 | Genencor International, Inc. | HPV CD8+ T-cell epitopes |
| ITRM20040091A1 (it) | 2004-02-19 | 2004-05-19 | Istituto Naz Per Le Malattie | Test immunologico rapido per la diagnosi ed il monitoraggio dell'infezione tubercolare. |
| ES2648792T3 (es) | 2004-04-28 | 2018-01-08 | Btg International Limited | Epítopos relacionados con la enfermedad celiaca |
| US10105437B2 (en) | 2004-04-28 | 2018-10-23 | Btg International Limited | Epitopes related to coeliac disease |
| EP1781313A4 (en) | 2004-06-17 | 2009-08-26 | Beckman Coulter Inc | MYCOBACTERIUM TUBERCULOSIS EPITOPES AND METHODS OF USE |
| GB0605474D0 (en) * | 2006-03-17 | 2006-04-26 | Isis Innovation | Clinical correlates |
| GB0619853D0 (en) | 2006-10-06 | 2006-11-15 | Oxford Immunotec Ltd | Preparation method |
| DE102007052517A1 (de) | 2007-10-29 | 2009-04-30 | Autoimmun Diagnostika Gmbh | ELISPOT-Verfahren mit zwei Filtersystemen |
| GB2455108A (en) * | 2007-11-28 | 2009-06-03 | Circassia Ltd | T-Cell dependent method for detecting non-allergic or intrinsic disorders |
| JP2012503206A (ja) | 2008-09-22 | 2012-02-02 | オレゴン ヘルス アンド サイエンス ユニバーシティ | Ｍｙｃｏｂａｃｔｅｒｉｕｍｔｕｂｅｒｃｕｌｏｓｉｓ感染を検出するための方法 |
| GB0906215D0 (en) | 2009-04-09 | 2009-05-20 | Lalvani Ajit | Diagnostic test |
| EP2501397B1 (en) | 2009-11-20 | 2017-10-04 | Oregon Health and Science University | Methods for producing an immune response to tuberculosis |
| IT1403092B1 (it) | 2010-12-01 | 2013-10-04 | Univ Degli Studi Modena E Reggio Emilia | Metodo per la diagnosi e/o il monitoraggio della mucormicosi. |
| WO2012103315A2 (en) | 2011-01-27 | 2012-08-02 | Osa Holdings, Inc. | Apparatus and methods for treatment of obstructive sleep apnea utilizing cryolysis of adipose tissues |
| FR2983213B1 (fr) | 2011-11-30 | 2015-03-06 | Commissariat Energie Atomique | Procede de mesure en temps reel des secretions individuelles d'une cellule |
| WO2013093512A2 (en) * | 2011-12-23 | 2013-06-27 | Retroscreen Virology Ltd | Vaccine - screening method |
| GB201122297D0 (en) | 2011-12-23 | 2012-02-01 | Retroscreen Virology Ltd | Vaccine - screening method |
| US9678071B2 (en) | 2012-01-12 | 2017-06-13 | Mayo Foundation For Medical Education And Research | Detecting latent tuberculosis infections |
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| CA2962920C (en) | 2014-10-01 | 2023-03-14 | Cryosa, Llc | Apparatus and methods for treatment of obstructive sleep apnea utilizing cryolysis of adipose tissues |
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| CN110073215B (zh) | 2016-09-22 | 2024-04-30 | 佩斯诊断公司 | 结核分枝杆菌蛋白诊断测定和结核病检测和诊断装置 |
| US10245320B2 (en) | 2016-09-30 | 2019-04-02 | Enzo Biochem, Inc. | Immunomodulatory pharmaceutical compositions and methods of use thereof |
| WO2018111536A1 (en) | 2016-12-14 | 2018-06-21 | Becton, Dickinson And Company | Methods and compositions for obtaining a tuberculosis assessment in a subject |
| WO2019018607A1 (en) | 2017-07-20 | 2019-01-24 | Enzo Biochem, Inc. | IMMUNOMODULATORY COMPOSITIONS AND METHODS OF USE THEREOF |
| KR102182555B1 (ko) * | 2019-03-14 | 2020-11-24 | 한국과학기술연구원 | T 세포 면역 반응 활성화를 위한 암 치료용 암항원 발굴 플랫폼 |
| US20220214332A1 (en) | 2019-05-30 | 2022-07-07 | Oregon Health & Science University | Methods for Detecting A Mycobacterium Tuberculosis Infection |
| CN111856020B (zh) * | 2020-06-19 | 2022-09-27 | 南方医科大学南方医院 | 乙型肝炎病毒（hbv）特异性t细胞检测方法及其应用 |
| CA3243280A1 (en) | 2021-12-30 | 2023-07-06 | Cryosa, Inc. | SYSTEMS AND METHODS FOR THE TREATMENT OF OBSTRUCTIVE SLEEP APNEA |
Family Cites Families (13)
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| CA1299099C (en) | 1986-03-06 | 1992-04-21 | Paul Richard Wood | In vitro assay for detecting cell-mediated immune responses |
| US5955077A (en) | 1993-07-02 | 1999-09-21 | Statens Seruminstitut | Tuberculosis vaccine |
| DK79893D0 (da) | 1993-07-02 | 1993-07-02 | Statens Seruminstitut | New vaccine |
| US20020136733A1 (en) | 1994-03-31 | 2002-09-26 | Hill Adrian Vivian Sinton | Malaria peptides |
| WO1997000067A1 (en) * | 1995-06-15 | 1997-01-03 | University Of Victoria Innovation And Development | Mycobacterium tuberculosis dna sequences encoding immunostimulatory peptides |
| DE19525784A1 (de) * | 1995-07-14 | 1997-01-16 | Boehringer Mannheim Gmbh | Autoreaktive Peptide aus der humanen Glutamin-Decarboxylase (GAD) |
| US6410252B1 (en) | 1995-12-22 | 2002-06-25 | Case Western Reserve University | Methods for measuring T cell cytokines |
| US5939281A (en) | 1996-09-16 | 1999-08-17 | Case Western Reserve University | Detecting alloreactivity |
| GB9624456D0 (en) | 1996-11-25 | 1997-01-15 | Isis Innovation | Assay method |
| CA2348475A1 (en) * | 1998-11-04 | 2000-05-11 | Isis Innovation Limited | Tuberculosis diagnostic test |
| US20020131976A1 (en) | 1998-12-23 | 2002-09-19 | Ajit Lalvani | Tuberculosis vaccine |
| WO2002054072A2 (en) | 2001-01-08 | 2002-07-11 | Isis Innovation Limited | Assay to determine efficacy of treatment for mycobacterial infection |
| GB0215710D0 (en) * | 2002-07-05 | 2002-08-14 | Isis Innovation | Diagnostic method |
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| US8617821B2 (en) | 2013-12-31 |
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| AU5063298A (en) | 1998-06-22 |
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