JP3476835B2 - Yacon / oolong blended food - Google Patents

Description

TECHNICAL FIELD The present invention relates to a dietary therapy for preventing or ameliorating dyslipidemia represented by diabetes mellitus and hyperlipidemia and a curing tea suitable for diet or a method of using the same. Contains a blended tea that is a mixture of above-ground yacon and oolong tea leaves originating in the Andes of South America, a drug for preventing or improving diabetes and dyslipidemia, which contains a water extract of the blended tea as an active ingredient, and a water extract of the blended tea The present invention relates to a food for preventing or ameliorating the same disease and a diet food.

BACKGROUND ART Diabetes is a disease caused by abnormal glucose metabolism, and it is estimated that there are currently 6 million patients in the country, and it is estimated that there are almost twice as many reserve troops.

Diabetes is roughly classified into insulin-dependent (IDDM) and non-insulin-dependent (NIDDM). Insulin-dependent is insulin caused by necrosis or dysfunction of pancreatic β cells by an autoimmune mechanism caused by a virus. It is a type that cannot synthesize and secrete, and accounts for less than 10% of patients. The insulin-independent type is a type that exhibits hyperglycemia due to insufficient insulin secretion or tissue insulin resistance caused by uncertain factors such as aging and stress, and 90% or more of patients include this type. Be done. Dietary therapy and exercise therapy are mainly used for the treatment of mild or moderate patients of this type, and the blood sugar level is stabilized by limiting the calorie of the meal and stimulating the metabolism of sugar by exercise.

However, if the rapid rise in postprandial blood glucose level and its continuation (postprandial hyperglycemia) continue for many years, vascular disorder is promoted with the deterioration of diabetes, and neurosis, nephropathy, retinopathy, and even myocardial infarction, There is a risk of developing complications such as stroke. Suppression of postprandial hyperglycemia is said to be effective in preventing the development of non-insulin-dependent diabetes mellitus, and α-glucosidase inhibitors such as acarbose are marketed as ethical drugs, but they are used as a dietary drug from the viewpoint of prevention or deterioration of diabetes. Further enhancement of therapy is desired.

Other than diabetes, stress is another factor that causes hyperglycemia. Today is also called a stressed society,
Many people, from children to adults, feel stress. In addition, there are not a few people who drank and eat under this stress. It is said that stress excites the sympathetic nerve and promotes the secretion of adrenaline or noradrenaline, which increases the blood sugar level. Therefore, there is a risk of becoming hyperglycemic if stressfulness in daily life and further excessive drinking / eating continue, leading to the development of complications similar to diabetes. Control is needed.

In addition, it is considered that abnormal lipid metabolism causes hypertriglyceridemia and hypercholesterolemia, that is, hyperlipidemia, and has a significant influence on the progression of arteriosclerosis. Arteriosclerosis is a disease that causes stenosis or occlusion of the lumen of the arterial wall and causes a decrease in blood flow, leading to pathological conditions such as transient cerebral ischemic attack, cerebral infarction, myocardial infarction, and angina pectoris. Mortality from cerebral cardiovascular disease accounts for about 40% of all mortality and is increasing in recent years.

Furthermore, abnormal lipid metabolism is also associated with obesity. Obesity is a state in which fat is excessively accumulated in the body, and the dietary habits of Japanese people are changing in the same manner as those in the West. Therefore, obesity has become a major problem in health measures. According to an epidemiological research report on obesity in 1995, BMI (Body Mass
Index) ≧ 26.4, 11.9% of men and 12.5% of women are obese in Japan, especially 20 in women.
It is said that the frequency of obesity increases rapidly from the teens to the sixties.

With the westernization of dietary content in recent years, it is said that in addition to an increase in the fat intake rate, particularly the animal fat intake rate of beef and pork, overeating and lack of exercise, including abnormal intake patterns, induce obesity. As a treatment for obesity, diet therapy such as ultra-low energy diet with reduced energy intake and exercise therapy are performed. However, there is a problem with these treatment methods, and even if the body weight is reduced by diet therapy or exercise therapy, if these treatment methods are stopped, the rebound phenomenon causes overeating again and the body weight increases. In addition, obesity has diseases such as diabetes, high blood pressure, heart disease, and fatty liver as complications of 2 to 3 times more than those of normal weight, and it is a malignant neoplasm that was not related to obesity before. It has been pointed out that the merger rate is significantly high.

As described above, abnormalities in sugar metabolism and lipid metabolism are associated with all diseases of adult diseases, and the drug and diet food thereof are desired from the viewpoint of prevention or deterioration of adult diseases.

Yacon is a plant of the Asteraceae family native to the Andes of South America, and the scientific name is Polymnia sonchifolia. Yarcon was introduced to Japan as a root vegetable in 1985 after passing through New Zealand, and then its usage was studied at domestic agricultural experimental stations and university faculties of agriculture.

In particular, the tuberous root was found to contain a large amount of fructooligosaccharides, and when it was used as raw root vegetables for eating, it was considered to be a diet diet or diabetic diet due to its low calories, and fructooligosaccharides and fructose were also studied. Has been considered as a natural raw material.

On the other hand, regarding the above-ground part, especially the foliage part, of yacon that reaches 2 m at the time of rooting, it is used as a feed for livestock at the place of origin or a water-soluble solvent extract has been proposed as a skin external preparation for anti-aging and whitening. (Japanese Patent Laid-Open No. 8-17
5964) other than that, the development of its application is not sufficient, and it is the only application related to diabetes that in the course of research on the use of yacon in Japan, it is possible to use naturally dried yacon stems and leaves as tea alone. It has been reported that the blood sugar level of diabetic patients is lowered [Agriculture and Horticulture, Vol. 64, Vol. 4].
No. 538 (1989)] only.

However, in the above report, specific data on blood glucose lowering of tea using stalks and leaves of yacon and its characteristics were not sufficiently shown. Therefore, the present inventors are interested in the effective use of the blood glucose level suppressing effect. As a result of repeated studies, it was found that taking it as tea at meal significantly suppressed postprandial hyperglycemia, but it had strong bitterness and astringency, leaving room for taste.

DISCLOSURE OF THE INVENTION The present inventors improved the taste by mixing oolong tea with the above-ground portion of yacon, and making the blended tea with 0.5 to 2.0 parts by weight of oolong tea leaves per 1 part by weight of the above-ground yacon portion as a dry weight ratio. However, surprisingly, by combining Yakon above-ground part and oolong tea leaves as described above, compared with the case where each of them is used alone, it suppresses blood glucose level after glucose absorption, It was found that the inhibitory effect on increase and the inhibitory effect on body weight increase leading to the prevention of obesity are clearly enhanced, and the present invention has been completed.

That is, the present invention contains a blended tea prepared by mixing Yacon aerial part and oolong tea leaves, a drug for preventing or improving diabetes and dyslipidemia containing the water extract of the blended tea as an active ingredient, and the water extract of the blended tea. The present invention provides a food for preventing or ameliorating the disease and a diet food.

In order to prevent and improve diabetes and dyslipidemia, and to take a diet for the purpose of daily intake of a green tea as a part of diet therapy, the taste occupies a very important factor. For the purpose of improving the taste in the case of, mixed tea with a different mixing ratio with oolong tea leaves was prepared, 4 g of mixed tea was decocted with 600 ml of boiling water, and a panel test of taste was carried out by 30 adults, as a result, dry weight As a result, it was found that 0.5 to 2.0 parts by weight of oolong tea leaves is preferable in terms of taste with respect to 1 part by weight of yacon above ground.

The yacon aerial part used in the present invention refers to any or all of leaves, stems or petioles, which is a term of plant morphology, and can be used regardless of the place of origin or harvest time.
Upon use, the yacon top part is dried in the sun for 1 day and then sun-dried for 2 days, and then it is chopped or powdered directly, or it can be used after roasting the dried top part if necessary. . When roasting,
Before mixing with oolong tea leaves, heat at an external temperature of 140-150 ℃,
From the viewpoint of taste, it is preferable to heat for several minutes while confirming the scent after the steam is released, but it is not limited to this.

Oolong tea is known as a semi-fermented tea having an action of improving lipid metabolism, and the oolong tea leaves used in the present invention can be used regardless of the origin, harvest time or grade.

Regardless of the temperature of the water, the blended tea of the present invention can be infused and taken in the same manner as ordinary tea in any of hot water, hot water and cold water. In particular, in order to control postprandial hyperglycemia, it is preferable to take it at the same time as the meal or within 2 hours before the meal.

Further, the compounded tea of the present invention is extracted with water regardless of the temperature, and the extract is freeze-dried, and dried using a method such as spray drying, as a powder or as fine granules, powders, granules, tablets, It can also be used as a drug or food by formulating it in the form of a coated tablet, capsule, soft capsule, syrup and the like.

These dosage forms can be prepared by pharmaceutically acceptable excipients, binders, lubricants, disintegrants, suspending agents, emulsifiers, preservatives, stabilizers, dispersants, etc., such as lactose and sucrose. , Starch, light anhydrous silicic acid, dextrin, crystalline cellulose, kaolin, calcium carbonate, talc, magnesium stearate, distilled water, or saline.

In addition, when preparing food, freeze-dried or spray-dried powder as it is or dissolved in fresh water to prepare a liquid agent, or drinks such as juice, liquor, beer and tea, noodles, bread, cooked rice, biscuits, etc. Processed grain products,
It can also be used by adding it to paste products such as sausages, hams and kamabokos, jams, jellies, gummy candy, gums, dressings, sprinkles and seasonings. The amount to be added can be adjusted appropriately, and can be added at an appropriate production stage.

When used as a powder, the dose and dose will vary depending on the patient's symptoms, age, weight, etc., but for adults with a body weight of 60 kg, 200 to 2,000 mg should be ingested or administered in several divided doses. You can

BEST MODE FOR CARRYING OUT THE INVENTION Next, the present invention will be described in detail with reference to Examples, Production Examples and Test Examples, but the present invention is not limited thereto.

[Example 1] A compounded tea prepared by mixing 1.5 g of Shikoku yakon leaf dried in the shade for 1 day in the sun and dried for 2 days and 2.5 g of oolong tea leaf from Fujian Province of China was prepared by a usual method. Tea bag
It was added to 600 ml of boiling water, decocted for 3 minutes, and then removed.
The decoction was concentrated under reduced pressure to about 50 ml and freeze-dried for 8 hours to obtain 1.1 g of a brown powder.

[Example 2] In the same manner as in Example 1, brown freeze-dried powder was prepared using 2.0 g of Yakon leaves from Shikoku and 2.0 g of oolong tea leaves from Fujian Province of China.
1.15 g was obtained.

[Example 3] In the same manner as in Example 1, a brown lyophilized powder was prepared using 2.5 g of Yakon leaves from Shikoku and 1.5 g of oolong tea leaves from Fujian Province of China.
1.13 g was obtained.

[Example 4] Example of tablet formulation using the freeze-dried powder of Example 1 (in 15 tablets) Lyophilized powder of Example 1 1,100 mg Crystalline cellulose 1,270 mg Partially pregelatinized starch 330 mg Lactose 1,365 mg Sucrose fatty acid ester 100 mg Hardened oil 35 mg [Example 5] Formulation example of a fine granule using the freeze-dried powder of Example 1 (2
Freeze-dried powder of Example 1 1,100 mg Cyclodextrin 1,640 mg Sucrose fatty acid ester 60 mg [Example 6] Formulation of a capsule using the freeze-dried powder of Example 2 (in 10 capsules) Freezing of Example 2 Dry powder 1,150 mg Lactose 1,690 mg Anhydrous calcium hydrogen phosphate 100 mg Sucrose fatty acid ester 30 mg Hardened oil 30 mg [Production Example 1] In the same manner as in Example 1, a lyophilized dry brown powder 2.5 was obtained using only 10.0 g of Shikoku yacon leaves. got g.

[Manufacturing Example 2] In the same manner as in Example 1, oolong tea leaves 1 produced in Fujian, China
2.6 g of brown lyophilized powder was obtained using only 0.0 g.

Next, the effects of the blended tea of the present invention on the blood glucose level will be described using the following Test Examples 1 and 2, and the effects on neutral fat and cholesterol, and further on the body weight will be described using Test Examples 3 and 4. . In addition, these test samples 1 to 3 correspond to the hot water extracts of Examples 1 to 3, and the samples of Comparative Examples 1 and 2 are the yacon leaves alone or the oolong tea leaves alone of the above Production Examples 1 and 2. Corresponds to hot water extract.

[Test Example 1] Glucose tolerance test (oral administration) Male Wistar rats (7 weeks old, 195.6 ± 1.5 g, 1
After fasting for 17 hours in 5 groups, a mixed solution of glucose (1.5 g / kg) dissolved in distilled water and a sample (1.0 g / kg) was orally administered using a rat oral probe. After administration, blood was collected from the tail vein every 15 minutes up to 120 minutes, plasma was prepared, and glucose concentration in plasma was measured using glucose B-test Wako (manufactured by Wako Pure Chemical Industries, Ltd.).

In the control group to which only glucose (1.5 g / kg) was orally administered, the glucose concentration in plasma showed a peak about 30 minutes after the administration, and then gradually decreased. On the other hand, when glucose and a test substance were orally administered at the same time, the peak value was lowered as shown in Table 1 below, and the blood glucose elevation suppressing effect was clearly observed in all the test substances. However, Samples 1-3 and Comparative Examples 1-2
There was no significant difference between the data.

Even when the glucose used in the glucose tolerance test was replaced with sucrose or starch, an inhibitory effect on the increase in blood glucose was observed in any of the hot water extracts of the blended tea of the present invention, the yacon leaf of Comparative Example, and the oolong tea leaf. . Therefore, the blended tea of the present invention significantly suppressed the increase in blood glucose after oral glucose loading regardless of the type of sugar to be loaded, and is therefore useful for the suppression of postprandial hyperglycemia.

[Test Example 2] Glucose tolerance test (subcutaneous administration) Male Wistar rats (7 weeks old, 198.9 ± 1.9 g, 1
After fasting for about 17 hours in 5 groups, glucose (1.5 g / kg) dissolved in distilled water was subcutaneously administered to the back of the rat, while a sample (1.0 g / kg) dissolved in distilled water was orally administered to the rat. Oral administration was performed using a sonde. After the administration, blood was collected from the tail vein over time similarly to Test Example 1, plasma was prepared, and the glucose concentration in the plasma was measured using Glucose B-Test Wako (manufactured by Wako Pure Chemical Industries, Ltd.).

In the control group in which glucose (1.5 g / kg) was subcutaneously administered and at the same time only distilled water was orally administered, the glucose concentration in plasma showed a peak at about 30 minutes after the administration as in Test Example 1 above.
Then it gradually diminished. On the other hand, when the sample was orally administered at the same time as the subcutaneous administration of glucose, the hot water extract of the blended tea of the present invention significantly lowered the peak value as shown in Table 2 below, and the blood glucose level suppressing effect was observed. However, the hot water extract of oolong tea leaves alone did not have any blood sugar level suppressing action, and the hot water extract of yacon leaves alone had only a weak blood sugar level suppressing action. The above-mentioned effects of tea are unexpected results, and it is clear that the combination of yacon leaves and oolong tea leaves is responsible.

[Test Example 3] ICR male mice (6 weeks old, 31.6 ± 0.3 g, 1 group 6 mice)
Each sample (1.5 g / kg) dissolved in distilled water was orally administered once a day for 7 days using a mouse oral probe. Incidentally, only distilled water was similarly orally administered to the control group.

During the test period, food and water were given ad libitum, and after fasting for about 16 hours after the final administration, blood was collected from the abdominal aorta under ether anesthesia, and then blood plasma was obtained using an automatic chemical analyzer (Hitachi 7070 type). The amount of sex fat was measured. The results are shown in Table 3.

As is clear from the results of Table 3, the blended tea hot water extract of the present invention significantly reduced the amount of triglyceride in plasma as compared with the control group. However, in the case of yacon leaves and oolong tea leaves alone, The hot water extract did not significantly change the plasma triglyceride content.

The above-mentioned action of the blended tea of the present invention is an unexpected result, and it is clear that it is caused by the combination of yacon leaves and oolong tea leaves.

[Test Example 4] Obese diabetic mice (KK-A ^y mice, 5 weeks old, 28.4, preliminarily fed with a basic feed (CE-2, manufactured by CLEA Japan, Inc.) for 1 week
(± 0.3 g), each group consisting of 7 animals, the group at the start of the experiment, the experimental group fed with the basic feed containing 1.5% of the sample, and the control fed with the basic feed containing 1.5% of cellulose in place of the sample The experimental group and the control group were divided into groups and fed for 5 weeks.

During the test period, food and water were given ad libitum, and body weight and food consumption were measured every week. The group at the start of the experiment was fasted at the start of the experiment, and the other 4 groups were fasted for about 17 hours 5 weeks after the start of the test. Blood was taken from the abdominal aorta under ether anesthesia, and the liver was taken out. The concentration and the amount of triglyceride in the liver were measured. The concentrations of triglyceride and cholesterol in plasma were measured using an automatic chemical analyzer (Hitachi 7070 type), and the triglyceride of liver was quantified by the method of Folch et al.

The results of weight change are shown in Table 4, and the results of neutral fat and cholesterol concentrations in plasma and the amount of neutral fat in liver at the start of the experiment and 5 weeks after the start of the experiment are shown in Table 5.
Shown in.

The body weight and the amount of change in the control group fed with the feed containing no sample for 5 weeks increased with age. Blended tea of the present invention,
Comparing the change in body weight of each test group fed with the feed containing the hot water extract of the yacon leaf alone and the oolong tea leaf of the comparative example with the control group, among them, the blended tea hot water extract of the present invention is particularly preferable. In the group, the increase in body weight was significantly suppressed without any influence on the food intake. The above-mentioned action of the blended tea of the present invention is an unexpected result, and it is clear that it is caused by the combination of yacon leaves and oolong tea leaves.

Compared with the KK-A ^y mouse (5 weeks old) at the start of the experiment, the plasma triglyceride and cholesterol concentrations and the triglyceride content of the liver in the control group fed with the sample-free diet for 5 weeks were Significantly higher levels of plasma triglyceride and cholesterol levels and liver triglyceride levels in the comparative group containing yacon leaf or oolong tea leaf hot water extract. Was.

However, no significant increase was observed in the group fed with the feed containing the hot tea extract of the present invention, and the suppression of the increase in plasma triglyceride and cholesterol levels and the suppression of the increase in triglyceride content in the liver were suppressed. Admitted.

The above-mentioned action of the blended tea of the present invention is also an unexpected result, and it is clear that it is caused by the combination of yacon leaves and oolong tea leaves.

[Test Example 5] In order to confirm the toxicity of the blended tea of the present invention, an acute toxicity test of ICR mice was carried out using the freeze-dried powder of Example 1 as a representative example, but death cases were observed at 4,000 mg / kg. There wasn't.

From the above results, the combination tea of the present invention, regardless of the method of sugar loading or the type of sugar to be loaded, significantly controls the blood sugar level and suppresses a rapid increase in blood sugar level such as postprandial hyperglycemia, Since it showed the effect of stabilizing the value, it is expected that the blended tea of the present invention has both an effect of suppressing sugar absorption and an effect of improving sugar metabolism.

Further, the blended tea of the present invention not only suppresses the increase of plasma neutral fat and cholesterol and liver triglyceride, but also has the action of lowering plasma neutral fat, further affecting the food intake. Since it also suppresses the increase in body weight without giving, it is expected to be effective not only for the prevention of abnormal glucose metabolism but also for the prevention of abnormal lipid metabolism.

Therefore, the combination tea of the present invention not only suppresses a rapid rise in blood sugar level due to meals but also prevents abnormal metabolism of sugar and fat, and thus suppresses excessive caloric intake of middle-aged and elderly diabetic and obese reserve troops in daily life. , Diabetes and obesity can be expected to be applied to diet therapy and supplementary use of these therapeutic agents, and diabetes and obesity, which are social problems reflecting the age of satiety, and abnormal glucose and lipid metabolism It is useful for prevention and amelioration of diseases related to, for example, hyperlipidemia, which is one of the risk factors for ischemic heart disease, arteriosclerosis, and so-called adult diseases such as liver damage, hypertension, and stress.

According to the present invention as described above, as a dry weight ratio,
By using the yacon-oolong blended tea in which 0.5 to 2.0 parts by weight of oolong tea leaves is mixed with 1 part by weight of yacon aerial parts, the taste is improved, and compared to the case where yacon aerial parts and oolong tea leaves are used alone. After the absorption of sugar, the inhibitory effect on blood sugar level, the inhibitory effect on the increase of neutral fat and cholesterol, and the preventive effect on obesity were clearly enhanced.

INDUSTRIAL APPLICABILITY The mixed tea of the present invention can be taken daily as a curing tea useful for blood sugar level control, prevention of lipid metabolism abnormality and diet, and diabetes or lipids containing the water extract of the mixed tea as an active ingredient. A drug for preventing / ameliorating metabolic disorders can be provided, and further, by adding the water extract to food, a food for preventing or improving the disease and a diet food can be obtained.

─────────────────────────────────────────────────── ─── Continuation of the front page (51) Int.Cl. ⁷ Identification code FI A61P 3/06 A61P 3/10 3/10 A23L 2/00 F (72) Inventor Michihiro Taka Takao, Oizumi-cho, Nerima-ku, Tokyo 33 No. 7 Inside the research institute of Zenyaku Kogyo Co., Ltd. (56) Reference JP-A-7-19664 (JP, A) JP-A-8-109178 (JP, A) Teruo Tsukihashi, for cultivation of yacon (Polymni a sonchifolia) Related research, agricultural work research, 1996, Vol. 31, No. 2, pp. 77-83 Agriculture and Horticulture, 1989, Vol. 64, No. 4, pp. 538-540, 1989 (58) Fields investigated (Int.Cl. ⁷ , DB name) A61K 35/78 A23F 3/14 A23L 1/307 A23L 2/38 A23L 2/52

Claims (6)

(57) [Claims] 1. A food for preventing or improving diabetes mellitus, preventing or improving lipid metabolism abnormalities, and comprising a dry tea containing 0.5 to 2.0 parts by weight of oolong tea leaves per 1 part by weight of yacon above as dry weight. 2. A drug for preventing or ameliorating diabetes, which comprises the water extract of the blended tea according to claim 1 as an active ingredient. 3. A food for preventing or improving diabetes which comprises the water extract of the blended tea according to claim 1. 4. A drug for preventing or ameliorating dyslipidemia, which comprises the water extract of the combined tea according to claim 1 as an active ingredient. 5. A food for preventing or ameliorating dyslipidemia, which comprises the water extract of the blended tea according to claim 1. 6. A diet food containing the water extract of the blended tea according to claim 1.