KR100609105B1 - Functional food for treatment of diabetes and preparing method thereof - Google Patents

Abstract

The present invention relates to an anti-diabetic functional formula comprising 40 to 45% by weight of silkworm, 40 to 45% by weight of red ginseng, 8 to 18% by weight of sewage and 2% by weight of small gallbladder, Wherein the composition further comprises 5 to 10 parts by weight based on 100 parts by weight of the composition of the present invention and the composition of the present invention. , An anti-diabetic functional formula excellent in therapeutic effect on diabetes and a method for producing the same can be obtained with little side effects.

Description

BACKGROUND OF THE INVENTION 1. Field of the Invention < RTI ID = 0.0 > [0001] < / RTI &

The present invention relates to an anti-diabetic functional formula and a method for preparing the same, and more particularly, to an anti-diabetic functional formula containing freeze-dried silkworm powder, red ginseng, sewage, small gall bladder, Lt; / RTI >

Because modern people are exposed to pollution and pollution, they are suffering from diverse diseases such as diabetes, hypertension, heart disease and obesity due to vascular disorders or hormone abnormalities. In addition, most of the modern people busy with daily routines are enjoying instant food that can not consume nutrition, and the occurrence of nutritional imbalance is widespread throughout society.

 In recent years, Korea has become a very desirable phenomenon in terms of the physical development of adolescents as the eating habits are westernized due to rapid economic growth and improvement of living standards. On the other hand, in adults, excessive intake of high calorie foods, lack of exercise, , The disease pattern is gradually becoming westernized mainly by adult diseases. Examples of representative adult diseases include hypertension, diabetes mellitus, obesity, hyperlipidemia (hypercholesterolemia), and especially diabetes is a cause of all chronic vascular diseases.

In Korea, the prevalence of diabetes mellitus was 0.5% of the total population before the 1970s, but the interest of the medical sector was low in the 1970s. In the 1980s, 2 ~ 3% ), It is estimated that more than 3 million people in the 2000s, and a considerable number of people living without knowing the onset of diabetes. If the potential diabetic patients are combined, the number of patients is enormous. After 20 years, the number is over 10 million, so it can not be serious.

Diabetes mellitus is not a large disease in itself, but rather a complication that arises from long-term illnesses such as diabetic neuropathy, retinopathy, cataract, nephropathy, etc. This is a major problem because patients can not only lead normal lives but also cause catastrophic results.

Diabetes is a disease in which glucose is not used as an energy source in the body because it lacks insulin secreted from the pancreatic β-cells or functions properly, and remains in the blood at high concentration and excreted in the urine.

Currently, commonly used diabetes drugs are broadly divided into oral hypoglycemic agents and insulin injections. In general, insulin injections are insulin-dependent patients who do not have insulin secretion in the body, or insulin-dependent patients who do not have the effect of taking oral hypoglycemic agents (insulin secretion, but whose use is not effective) I will. Oral hypoglycemic agents are used in patients with non-insulin dependent diabetes mellitus who do not benefit from diet and exercise.

Most of such diabetes mellitus is to treat diabetes through medication and diet therapy in hospitals, or to maintain a healthy state by lowering blood sugar. However, such medication has a risk of adverse effects such as latic acidosis, pancreatic cancer, pain, blood stain, etc., and when it is overdosed, Although the descent effect appears quickly, there is a problem that the effect is not continuous.

The above-mentioned approaches for the treatment of diabetes are western medical methods that artificially lower blood glucose levels. Therefore, it is necessary to develop oral antidiabetic functional foods which can reduce such side effects because of the large side effects.

Recently, studies have been actively conducted to treat or alleviate diabetes by using natural foods or herbal medicines, which are effective for diabetes. Examples thereof include health supplement for diabetes (Korean Patent Application No. 2001-0032751) manufactured by using silkworm powder, yellow bean flour, ginger bract, black quasi-fever, etc., a health supplement capsule containing chufa and silkworm 2002-0024389). However, these effects are mild, and even if effective, temporarily relieve the symptoms of diabetes or lower blood sugar levels. In addition, most of these products are made of one or a combination of natural foods and herbal medicines, which are effective for diabetes without proving correct efficacy or clinical data. Therefore, there is a concern that the influence on the human body is unclear, .

On the other hand, the silkworm has been reported to inhibit the increase of blood sugar by preventing the decomposition of starch into monosaccharides at one time because it contains an alpha-glucosidase inhibitor which is an enzyme that decomposes polysaccharides into monosaccharides , And also has a blood pressure lowering effect (Korean sericulture).

It is known that red ginseng contains adenosine and pyroglutamic acid and is known to lower the blood sugar level and promote insulin secretion and is also known to inhibit aging of pancreatic beta cells (Yoshida, Yoshida, Japan) Ph.D., Journal of Korean Ginseng and Pharmacology).

It is known that Sasao has the effect of protecting blood circulation by controlling blood sugar (Korean Journal of Pharmacognosy).

It is known to be effective for the weakness of the body, weakness of the tongue, pulmonary tuberculosis, diabetes, nourishment, hypertrophy and fatigue recovery.

Small gallbladder is known to be effective in improving heart and liver function, brain function improvement, and nervous breakdown.

Glycyrrhiza is known to have hypoglycemic effect, sedative effect, anticancer effect (Journal of Pharmacognosy), and Baekbongul has a known effect of lowering blood sugar (Dongbibogam, Basho gangmok).

It is an object of the present invention to provide an antidiabetic functional formula and a method for producing the same which overcome the problems of the above-mentioned conventional diabetes mellitus treatment method and have excellent side effects on human body and have a therapeutic effect on diabetes mellitus.

In order to achieve the above object, according to the present invention, there is provided an anti-diabetic functional formula comprising 40 to 45% by weight silkworm, 40 to 45% by weight red ginseng, 8 to 18% by weight sodium chloride and 2% Wherein the composition further comprises 5 to 10 parts by weight per 100 parts by weight of silkworm, red silkworm, dung, and small gallbladder, and a method for producing the same.

The present invention will be described in more detail as follows.

In order to overcome the problems of the prior art, the inventors of the present invention have continuously tested various herbal materials. As a result, silkworm, red ginseng, seaweed and small gall bladder were mixed at a specific ratio, It has been found that an excellent antidiabetic effect can be obtained while minimizing side effects in the case of further mixing with a certain ratio. Thus, the present invention has been completed.

According to the present invention, it is preferable from the viewpoint of physiological effect that silkworms of 5 days old and 3 days old are lyophilized and pulverized.

In case of mixing with the lyophilized powdered silkworm, it is preferable to use powdered powder for easy mixing and ingestion.

When used as a filtrate by hot water extraction of red ginseng, daphnia, small gallbladder, liquorice, white bream, and brine, it may be used as a dry lump without powdering, or in the form of flakes of 1 to 5 mm Is preferably used.

It is preferable that the content of each raw material of the functional formula including silkworm, red ginseng, sewage and small gall bladder is 40 to 45% by weight of silkworm, 40 to 45% by weight of red ginseng, 8 to 18% by weight of sewage and 2% At this rate, however, the synergistic effect is maximized and there are few side effects.

In the case of a functional formula further comprising the raw materials other than the above raw materials, Baekbyeonggi, and Zygo, the above ratio is maintained for silkworm, red ginseng, sewage and small gallbladder. In addition, it is preferable that the content of liquorice, bamboo shoots and plum is 5 to 10 parts by weight per 100 parts by weight of silkworm, red ginseng, sewage and small gallbladder.

The functional formula according to the present invention can be prepared by simply pulverizing and mixing the raw materials.

Alternatively, the freeze-dried silkworm powder can be prepared by a post-blending of the freeze-dried silkworm powder to the filtrate obtained by hot-water-extracting the raw materials except for the freeze-dried silkworm powder.

More specifically, 5 to 10 times as much water as the raw material is added to red ginseng, sewage, small gallbladder, alternatively, liquorice, white bream and plum, and the mixture is subjected to hot water extraction at 70 to 90 ° C for 2 to 3 hours. At this time, it is preferable that the raw materials are used in the form of bean curds or flakes of 1 to 5 mm as described above.

It is then filtered at 40-200 mesh to separate the filtrate. If necessary, it can be concentrated to the required extent to produce a concentrate.

The filtrate or its concentrate is then sufficiently cooled. If necessary, it is lyophilized and milled according to conventional freeze-drying methods.

Then, the freeze-dried silkworm powder is finally subjected to final solvation to obtain a functional formula. At this time, when the filtrate or its concentrate is lyophilized and pulverized and the lyophilized silkworm powder is later mixed, the functional formula can be obtained in powder form.

The above functional form can be formulated into preparations such as pills, tablets, capsules, solutions and suspensions according to a conventional method.

In the production of the pellets, the small gallbladder is used as a cyclic agent.

In addition, according to the present invention, the functional formula can be mixed at the time of the production of the food, and the antidiabetic functional food such as beverage, ramie noodle, and snack can be prepared according to a conventional method.

For example, the functional formula may be added at the time of adding a face or soup, and a snack may be prepared by adding a seasoning or a raw material to the snack.

The following examples are intended to further illustrate the present invention and are not intended to limit the scope of the present invention.

(Example 1)

Powdered functional formulas were prepared by thoroughly mixing 42 kg of freeze-dried powder of silkworm of 3 days old, 42 kg of red ginseng powder, 14 kg of sewage powder, 10 kg of powdery mildew, 2 kg of small gallbladder powder, 10 kg of ground powder and 10 kg of balsam powder with a mixer.

(Example 2)

Powder type functional formulas were prepared by mixing 45kg of freeze - dried powder of silkworms of 5 days old, 45kg of red ginseng powder, 8kg of sewage powder and 2kg of small gallbladder powder using a mixer.

(Example 3)

Water was added to 45 kg of red ginseng, 8 kg of seaweed and 2 kg of small gall bladder, and the mixture was subjected to hot water extraction at 80 ° C. for 3 hours, followed by filtration through 120 mesh to separate the filtrate. After sufficiently concentrating and cooling the filtrate, 45 kg of the lyophilized silkworm powder was finally mixed, and then a cyclic functional formula was prepared according to a conventional method.

(Example 4)

Water was added to 45 kg of red ginseng, 8 kg of seaweed and 2 kg of small gall bladder, and the mixture was subjected to hot water extraction at 80 ° C. for 3 hours, followed by filtration through 120 mesh to separate the filtrate. This was rapidly frozen at -70 ° C for 3 hours and then pulverized by freeze-drying at a vacuum degree of 0.4 mbar, a cooling trap temperature of -40 ° C, and a product temperature of 60 ° C. Then, 48 kg of freeze-dried silkworm powder was mixed with each other to prepare a powder-type functional formula.

(Comparative Example 1)

A functional formula was prepared in the same manner as in Example 1 except that only 42 kg of the lyophilized powder of silkworm of 5 days old and 3 days old was used as the raw material.

(Comparative Example 2)

A functional formula was prepared in the same manner as in Example 1 except that only 42 kg of red ginseng powder was used as a raw material.

(Comparative Example 3)

A functional formula was prepared in the same manner as in Example 1, except that only 14 kg of Sasa powder was used as a raw material.

(Comparative Example 4)

A functional formula was prepared in the same manner as in Example 1 except that only 2 kg of small chalk powder was used as a raw material.

(Comparative Example 5)

A functional formula was prepared in the same manner as in Example 1, except that 10 kg of powdery mildew powder, 10 kg of plum powder, and 10 kg of bale powder were used as raw materials.

(Comparative Example 6)

A functional formula was prepared in the same manner as in Example 1, except that 42 kg of freeze-dried powder of silkworm, 5 days old, 3 days old, and 42 kg of red ginseng powder were used.

(Comparative Example 7)

A functional formula was prepared in the same manner as in Example 1, except that 14 kg of sucrose powder and 2 kg of small gallbladder powder were used as raw materials.

(Comparative Example 8)

A functional formula was prepared in the same manner as in Example 1, except that 80 kg of freeze-dried powder of silkworm, 3 days old at 5 days, 80 kg of red ginseng powder, 14 kg of sewage powder and 2 kg of small gallbladder powder were used.

(Comparative Example 9)

A functional formula was prepared in the same manner as in Example 1, except that 1 kg of freeze-dried powder of silkworm, 3 days old at 5 days, 1 kg of red ginseng powder, 14 kg of sodium chloride powder, and 2 kg of small gallbladder powder were used.

(Comparative Example 10)

A functional formula was prepared in the same manner as in Example 1, except that 42 kg of the lyophilized powder of silkworm 3 days old at 5 days, 42 kg of red ginseng powder, 70 kg of sewage powder and 70 kg of small gallbladder powder were used.

(Comparative Example 11)

A functional formula was prepared in the same manner as in Example 1, except that only 42 kg of freeze-dried powder of silkworm, 5 days old and 3 days old, 42 kg of red ginseng powder, 0.2 kg of sewage powder, and 0.1 kg of small gallbladder powder were used.

(Test Example 1)

Blood glucose lowering effect test

In order to test the hypoglycemic effect, a model animal of type 2 diabetes (non-insulin dependent diabetes mellitus) (animal) was set up and a commercially available feed for mouse (constituents: corn, soybean, wheat, tallow, crude fat, Calcium, phosphorus, etc.) and crude protein (25% or more).

Type 2 diabetes is not known for certain mechanisms, but due to obesity, increased resistance to insulin in the peripheral tissues occurs, resulting in hyperinsulinemia. The beta cells of type 2 diabetic patients are less responsive to insulin secretion due to elevated blood glucose levels, and do not recognize the excretion of sugar into the urine and continue to secrete insulin.

360 KKA ^Y mice expressing these symptoms were divided into 18 groups (control group, test group 1 to 17) in 20 rats. In the control group, only physiological saline was administered at a dose of 10 ml / kg, and the functional formulas of the present invention prepared in Examples 1 to 4 were orally administered to test groups 1 to 4 at a dose of 0.5 g / kg / day for 5 weeks . In test group 5, gliquidone was administered at a dose of 0.5 g / kg / day for 5 weeks, and test group 6 was administered at a dose of 0.5 g / kg / day for 5 weeks. In the test groups 7 to 13, the functional formulas prepared in Comparative Examples 1 to 7 and the functional groups prepared in Comparative Examples 8 to 11 in Test Groups 14 to 17 were administered at a dose of 0.5 g / kg / day for 5 weeks, respectively. Blood was collected from the orbital vein of the mouse before and 2 hours after the drug administration for the control and test groups, respectively, and glucose was measured using a glucose-meter (glucose unit: mg / dl) ).

The measurement results are shown in Table 1 below.

division Before administration After administration Control group 162 ± 23.4 172 ± 22.5 Test group 1 161 ± 23.2 115 ± 12.6 Test group 2 161 ± 23.2 116 ± 12.4 Test group 3 161 ± 23.2 118 ± 11.6 Test group 4 161 ± 23.2 119 ± 11.8 Test group 5 162 ± 22.1 112 ± 12.2 Test group 6 162 ± 22.1 114 ± 12.1 Test group 7 161 ± 23.2 130 ± 12.6 Test group 8 161 ± 23.2 135 ± 12.8 Test group 9 161 ± 23.2 148 ± 20.3 Test group 10 161 ± 23.2 153 ± 20.3 Test group 11 161 ± 23.2 150 ± 20.8 Test group 12 161 ± 23.2 124 ± 20.2 Test group 13 161 ± 23.2 145 ± 18.8 Test group 14 161 ± 23.2 141 ± 20.8 Test group 15 161 ± 23.2 159 ± 20.3 Test group 16 161 ± 23.2 140 ± 20.5 Test group 17 161 ± 23.2 132 ± 20.4

According to the above Table 1, it can be seen that the functional formulas (test groups 1 to 4) of the present invention show a significant blood glucose lowering effect on type 2 diabetes when compared with the control group.

In addition, the functional formulas of the present invention can be compared with glycidon (test group 5), which is a representative sulfonylurea type diabetes treatment drug already proven efficacy, and Chinese herbal consonant (test group 6), a herbal preparation for treating diabetes, Similar blood glucose lowering effect can be seen.

The functional formulas in which the specific medicinal materials according to the present invention are mixed in a specific ratio can be obtained when the medicinal materials are used singly or in part (Test Groups 7 to 13), or when they are mixed outside the ratio (Test Groups 14 to 17) , The blood glucose lowering effect is excellent.

(Test Example 2)

Acute toxicity test

The LD ₅₀ value, which is a reference value for acute toxicity, was determined for the composition (functional formula) of the present invention using an experimental animal.

As an experimental animal, 100 ICR mice were used and fasted for 1 day prior to the experiment. These mice were divided into 5 groups (20 to 20 mice). As a control group, 10 mg / kg of physiological saline alone was administered to the group of mice, and the composition of the present invention prepared in Example 1 was administered at a dose of 10 g / kg / day. In the multi-dose group, the composition of Example 1 was administered at a dose of 30 g / kg / day, the lavage at the dose of 60 g / kg / day, the composition of Example 1 at a dose of 60 g / .

The animals of Groups I and II were orally administered with the compositions of the present invention dissolved in physiological saline. Twenty-four hours after oral administration, the number of dead animals in each group was determined. As a result, there were no dead mice in Naji and Daigun, 1 in lagoon and 2 in marine.

In order to obtain the LD ₅₀ , 50% of the experimental animals, that is, 10 mice, should be dosed at an increased dose. However, the composition of the present invention is insoluble in water. To dissolve more than 100 g / kg, It is too large to be administered to experimental animals.

As a result, no mice were found to have adverse effects such as hypoglycemia in the rats fed the functional formula of the present invention, and LD ₅₀ was estimated to be 100 g / kg or more.

The administration of 100 g / kg / day showed the effect of administering the composition of the present invention at 7 kg or more per day in terms of 70 kg body weight, so that the functional formula of the present invention was judged to be a safe composition having substantially no toxicity.

According to the present invention, it is possible to obtain an anti-diabetic functional formula having excellent therapeutic effects on diabetes and a method for producing the same, with little or no side effects, which is a new prescription different from existing functional formulas.

Claims (15)

An antidiabetic functional formula comprising 40 to 45% by weight silkworm, 40 to 45% by weight red ginseng, 8 to 18% by weight of tap water and 2% by weight of small gallbladder. The method according to claim 1, Wherein the composition further comprises 5 to 10 parts by weight of each of 100 to 100 parts by weight of silkworm, white bream, and ziplo, silkworm, red ginseng, sewage, and small gallbladder. Adding 10 times water to the raw material to 40 to 45% by weight of red ginseng, 8 to 18% by weight of seaweed, and 2% by weight of small gall bladder, and conducting hot water extraction at 70 to 90 ° C for 2 to 3 hours; Filtering the obtained hot water extract to 40 to 200 mesh to separate the filtrate; Wherein the freeze-dried silkworm powder comprises 40 to 45% by weight of the freeze-dried silkworm powder. The method of claim 3, And concentrating the separated filtrate. ≪ Desc / Clms Page number 19 > The method of claim 3, Further comprising the step of freeze-drying and pulverizing the separated filtrate. 5. The method of claim 4, And then lyophilizing and pulverizing the concentrated liquid. The method according to any one of claims 3 to 6, The method for producing an anti-diabetic functional food according to claim 1, wherein, in the step of extracting hot water, 5 to 10 parts by weight of scutellum, 5 to 10 parts by weight of scutellum are added to 100 parts by weight of silkworm, red ginseng, An anti-diabetic functional formula produced according to any one of claims 3 to 6. An anti-diabetic functional formula in which the anti-diabetic functional formula of claim 1 or 2 is formulated in the form of powder, pill, tablet, capsule, liquid or suspension. An anti-diabetic functional food characterized by comprising the anti-diabetic functional formula of claim 1 or 2. An antidiabetic functional formula produced according to claim 7. The anti-diabetic functional formula of claim 8, which is formulated in the form of powder, pill, tablet, capsule, liquid or suspension. An anti-diabetic functional formula of claim 11 formulated in the form of a powder, pill, tablet, capsule, solution or suspension. An anti-diabetic functional food characterized by comprising the anti-diabetic functional formula of claim 8. An antidiabetic functional food characterized by comprising the anti-diabetic functional formula of claim 11.