JP4516958B2 - Anti-diabetic composition - Google Patents

Description

TECHNICAL FIELD The present invention relates to an anti-diabetic composition, and more particularly to a preventive and therapeutic agent for diabetes and a health food containing petals and / or extracts thereof of the asteraceae plant safflower ( Carthamus tinctorius L. ) as an active ingredient. . The present invention also relates to a preventive and therapeutic agent for diabetes and a health food containing as active ingredients a red pigment carthamin, a yellow pigment safflower yellow, and saflomin A contained in safflower petals.

In recent years, high economic growth, improved living standards, and westernization have shown very favorable results in youth physical development, while adults are suffering from excessive intake of high-calorie foods, lack of exercise and complex industrial society. Diseases are becoming more and more westernized due to the stress that arises.
Examples of typical adult diseases include hypertension, diabetes, obesity, hyperlipidemia (hypercholesterolemia), and particularly diabetes is considered to be the cause of all chronic vascular diseases. Diabetes is one of the most common lifestyle-related diseases in Japan.
Diabetes is characterized by excessively high blood sugar levels or sustained hyperglycemia when sugars derived from carbohydrates taken from the diet are absorbed from the digestive tract and enter the blood. Diabetes often accompanies obesity and develops through a mild rise in blood glucose called the “pre-diabetes group”.
Among diabetes, insulin-independent type II diabetes has been increasing in recent years. Type II diabetes often develops due to lack of exercise or an irregular diet. For treatment, sulphonium urea preparations that promote insulin secretion, α-glucosidase inhibitors that suppress postprandial hyperglycemia, or thiazolidine preparations that improve insulin resistance are recently used. Since a preparation requires a prescription, it is not easily available, and may have various side effects depending on the administration or administration of the preparation. Diabetes therapeutic agents including α-glucosidase inhibitors have recently been reported to cause serious liver damage, and strict management and guidance from doctors are required for their use. Therefore, what is easily available and has as few side effects as possible is desired.

What is easily available and has few side effects includes so-called health foods that use functional substances derived from natural products as active ingredients. As an example of such a health food, there is a food in which an indigestible dextrin having an action of reducing the amount of absorbed sugar from the small intestine is blended as a functional substance derived from a natural product. As approved.
There are also foods containing plant Salacia reticulata having an α-glucosidase inhibitory action as an active ingredient and “special health foods” containing an active ingredient polyphenol of the leaves of plant guava ( Psidium guajava L. ).
A food containing such a substance derived from a natural product can be obtained in combination with a regular meal because it can be easily obtained without a prescription and is effective for early treatment of diabetes.
In addition, patent reports on health foods containing plant-derived ingredients for the purpose of preventing diabetes are also found. For example, Patent Document 1 discloses an anti-diabetic agent derived from an indigo plant belonging to the genus plant, Patent Document 2 refers to an anti-diabetic agent derived from the leguminous plant Fanella lingua, and Patent Document 3 refers to a plant belonging to the genus Lichenaceae. And the like preventive and therapeutic agents for diabetes. Many health foods with anti-diabetic effects are recognized, but there are many that are questionable in effectiveness, and there are hardly any specific foods that have been proven to be effective or effective in the body.
Japanese Patent Laid-Open No. 2002-179587 JP 2002-332239 A JP 2003-81858 A

  The object of the present invention is that it is easily obtained and ingested in daily eating habits, thereby suppressing postprandial hyperglycemia, effective in preventing the onset of diabetes and alleviating symptoms, effective from natural plants with few side effects A novel anti-diabetic agent comprising an ingredient, which uses a plant whose effective action (in particular, exhibits an inhibitory action on increase in blood glucose level after meals) as an active ingredient. It is to provide a diabetic agent.

In order to achieve the above object, the present inventors searched for various unknown plants and made various evaluations thereof. As a result, the petals of the Asteraceae safflower ( Carthamus tinctorius L. ) and / or extraction thereof. Or the red pigment cartamine and the yellow pigment safflower yellow contained in safflower petals have been found to be effective as preventive and therapeutic agents for diabetes and health foods.
Then, in vivo, rat sugar ( Carhamus tinctorius L. ) petals and / or extracts thereof, or the yellow pigment safflower yellows typified by safromin A, the red pigment cartamine contained in the safflower petals, were analyzed in vivo. Alternatively, in the starch load test, it was confirmed that a strong blood glucose level increase inhibitory effect was exhibited, and the present invention was completed.
Therefore, the present invention is for antidiabetics containing the petal and / or extract of the safflower plant safflower ( Carthamus tinctorius L. It relates to a composition.

  The safflower petals and / or extracts thereof of the present invention have an effect of suppressing the increase in blood sugar at the time of sugar load, and thus not only suppress the hyperglycemia state after eating and drinking due to overeating and unbalanced diets, but also lifestyle-related diseases. It can also be effectively used as a therapeutic agent for diabetes, which can be said to be representative. In addition, since it is a natural drug derived from plants based on long experience of use, there is no worry about side effects and it can be used at low cost, so it is effective for diet and diabetes prevention. Moreover, the safflower petal powder and / or extract of the present invention can be easily ingested as a health food, for example, in the form of confectionery, beverages, etc., and can be easily taken for a long time. You can continue to take it continuously.

Safflower is a plant of the first to second year of the genus Safflower, and its scientific name ( Carthamus tinctorius L. ) represents the meaning of dyeing in both genus name and species name. It has been cultivated with Murasaki as a representative natural dye material. In Japan, it is widely cultivated in the Mogami River basin, Yamagata Prefecture, but recently safflower is imported from China. The safflower petal pigments include the yellow safflower yellows that can be used as food pigments, as well as the red pigment carthamin, which is used as a cosmetic material for lipsticks and cheeks. Has crossed. Safflower yellows is a generic name for safflower yellow pigments, and safflower yellows include saflomin A, safflower yellow B, saflomin C, precursor, and the like. On the other hand, safflower has long been used as a medicinal herb, and its effects on irregular menstruation, coldness, menopause, blood circulation disorders, etc. have attracted attention, and in recent years its pharmacological action has been reviewed.

  Yamagata Institute of Laboratories (21) 9-1 (1988), Biopharmaceutical Journal (43) 331-338 (1989), Biopharmaceutical Journal (45) 306-310 (1991), Urakami Foundation Research Report (6) 88 -100 (1998), Yamagata Institute of Laboratories (32) 12-16 (1999), Yamagata Institute of Laboratories (33) 9-13 (2000), Yamagata Public Health Society Lecture (27) 45-46 ( 2001) reported the effectiveness of safflower alcohol extract in suppressing the increase of high cholesterol, sleep prolongation, superoxide scavenging activity, arteriosclerotic index lowering action, blood lipid lowering action, anti-inflammatory action, etc. ing.

  In addition, in the patent, Japanese Patent Laid-Open No. 2001-346555 has an effect of effectively reducing active oxygen in the extract of safflower cotyledons and stems, and Japanese Patent Laid-Open No. 2000-236838 has red bean cedar as a main raw material. The effect of obstetrics and gynecology disease on foods with added safflower petal dry powder is disclosed in JP 2001-506589 A, in which a mixed composition containing 17 kinds of natural drugs such as cordyceps and safflower has an effect for treating diabetes, In JP-A-7-322825, menthol was added to the hot water extract of safflower as a health ice cream to prevent diabetes including ginseng extract using a greening part of safflower sprouts. It has been reported as a poultice. JP 2003-40780 discloses the effect of suppressing hypercholesterolemia on the safflower petal red pigment cartamine.

  However, anti-diabetic studies on safflower petals or their extracts, as well as the red pigment carthamin and yellow pigment safflower yellow contained in safflower petals have not been conducted in the past. There were no reports on antidiabetic activity.

The anti-diabetic composition of the present invention contains, as an active ingredient, the petal and / or extract of the safflower plant ( Carthamus tinctorius L. ), or the red pigment cartamine and yellow pigment safflower yellow contained in the safflower petal. contains.
The safflower that can be used in the preventive and therapeutic agent for diabetes of the present invention may be a fresh flower or a dried flower, and examples thereof include domestic “most safflower” and Chinese, Israeli, American and Indian safflowers. Any type of safflower is not limited. Furthermore, safflower yellow pigment preparations and red pigment preparations that have already been commercialized may be used. Regardless of petal lot differences such as safflower petal type, place of origin, time of purchase, storage state, etc., it is possible to stably provide a high blood sugar level increase inhibitor.

Unless a commercially available safflower yellow pigment preparation or red pigment preparation is used, the active ingredient is generally prepared by the following method.
First, raw or dried petals of safflower ( Carthamus tinctorius L. ), which is a raw material, is pulverized using a general pulverizing means. Here, the pulverization is not particularly required until it is finely divided, and may be performed to such an extent that effective components can be sufficiently eluted from the safflower petals (for example, a size of about 5 mm or less). This pulverized product may be used as it is as an active ingredient of an antidiabetic composition, or a petal that is further pulverized into a powder form may be used as an active ingredient.
When making an extract, this pulverized product is extracted by adding to a solvent at room temperature or warming. Examples of the extraction solvent include alcohols having 1 to 8 carbon atoms such as methanol and ethanol, water, a mixed solvent of water and the above alcohols, ethyl acetate, and acetone. In particular, it is preferable to use water or an alcohol solvent as the extraction solvent, and most preferably, water, methanol, or ethanol is used. The extraction solvent is suitably used in an amount of about 2 to 100 times, preferably about 2 to 50 times the weight of the safflower petals. Extraction is performed by heating to about 25 to 80 ° C. for about 1 to 10 hours, or by immersing the plant for about 1 to 10 days at a cooling temperature of about 5 to 25 ° C. with or without shaking. Product can be prepared.
The extraction temperature and extraction time may vary depending on the extraction solvent used. For example, when water-containing methanol is used as the extraction solvent, it is preferable to perform heat extraction at a temperature of about 30 to 80 ° C. for about 3 to 24 hours.
After the extraction, the extraction solvent is evaporated under reduced pressure (for example, about 750 mmHg or less), and the residue is concentrated and solidified to obtain an extract as an active ingredient. Alternatively, the extract extracted with water or warm water is used after being pulverized by treatment by freeze-drying.

  Extract extracts or freeze-dried products prepared in this way can exert a strong inhibitory effect on the increase in blood glucose level if used as they are, but if necessary, they can be further purified to further increase their activity and action. Is possible. For example, a preparation obtained by freeze-drying a water extract from a safflower petal obtained by the above procedure contains yellow pigment safflower yellows, and this preparation exerts a blood glucose rise inhibiting effect more strongly. I can do it. Further, the yellow pigment safflower yellows obtained as described above are further purified by a known method, and a highly pure yellow pigment saflomin A or the like also exerts a strong blood glucose level inhibitory action, thereby preventing and treating diabetes, Can be used as a health food.

On the other hand, the remaining water-insoluble matter or safflower petals obtained by water extraction from safflower petals were treated under basic conditions in an aqueous medium using wood ash, baking soda solution, etc., which were traditionally used, and then insoluble matters were removed. The precipitate obtained by treating the liquid layer part under an acidic condition in an aqueous medium using brewed vinegar, acetic acid, citric acid, etc. contains the red pigment cartamine, and exerts a strong effect on suppressing blood sugar elevation. Here, the aqueous medium means water or a mixed solvent of water and an organic solvent soluble in water. Further, the highly purified red pigment cartamine, which is further purified by a known method from the precipitate containing the red pigment cartamine obtained in this way, exhibits a strong blood glucose level inhibitory action and has a prophylactic and therapeutic agent for diabetes. Can be used as a health food. Examples of the purification treatment method include a method using a chromatographic method, an elution method using an ion exchange resin, a recrystallization method or the like alone or in combination.
For example, examples of the chromatographic method include normal phase chromatography, reverse phase chromatography, high performance liquid chromatography, centrifugal liquid chromatography, column chromatography, thin layer chromatography and the like, or a method using a combination thereof. It is done. In this case, purification conditions such as a carrier and an elution solvent can be appropriately selected according to various chromatographies. For example, a chloroform-methanol solvent can be used for normal phase chromatography, and a water-methanol solvent can be used for reverse phase chromatography.
In addition, as an elution method using an ion exchange resin, the obtained extract is diluted / dissolved in water or lower alcohol, and this solution is brought into contact with the ion exchange resin to be adsorbed, followed by lower alcohol or water. The method of eluting is mentioned. The lower alcohol used in this case is as described above, and methanol is particularly preferable. The ion exchange resin is not particularly limited as long as it is usually used for purification treatment in the field. For example, a porous crosslinked polystyrene-based resin having a large network structure, amberlite, cellulose, etc. Is mentioned. Polyamide column chromatography is also used as an effective elution method for aqueous solution components.

When used as a prophylactic and / or therapeutic agent, the amount of safflower petal powder and / or extract used varies depending on symptoms, but for example, 0.1 to 10 g of safflower petals obtained by the above method per day for an adult, The extract is preferably 1 to 1000 mg depending on the degree of purification and water content, and preferably 1 to 1000 mg of pigments such as the red pigment carthamin itself, safflower yellows, and saflomin A.
The anti-diabetic composition of the present invention comprises, based on the total weight, petals of the safflower plant safflower, extracts from safflower petals, or the red pigment carthamin itself, or the yellow pigment saflomin A and safflower yellows themselves. It contains at least 1 to 40% by weight, preferably 2 to 20% by weight.

Safflower petals and / or extracts thereof can also be used in health foods.
Health food means a food that is more active than ordinary food, and is intended for health, health maintenance and promotion, for example, liquid or semi-solid, solid products such as cookies, Examples include confectionery such as rice crackers, jelly, yokan, yogurt and manju, soft drinks, nutritional drinks, soups and the like. Moreover, it may be decocted as it is to make a tea preparation. In the production process of these foods or to the final product, the above powder, extract and the like can be added by mixing, coating, spraying or the like to obtain a health food. The health food of the present invention includes, for example, 0.1 to 5 g of safflower petals obtained by the above method, 1 to 500 mg of an extract thereof, or red pigment carthamin itself, safflower yellow itself, It is preferable to contain in the quantity which can ingest 1-500 mg of pigment | dyes.
The health food of the present invention comprises a safflower petal, a fraction containing a safflower petal extract or a red pigment cartamine obtained from the safflower petal, a red pigment cartamine itself, or a yellow pigment safflower yellow. Or 30% by weight, preferably 1 to 10% by weight, within a range that does not adversely affect the taste and appearance of the food.

  In addition to the above active ingredients in anti-diabetic agents, other known compounds commonly used in the pharmaceutical field and compounds necessary for molding into a form suitable for oral administration, such as lactose, starch, hydroxypropylcellulose, kaolin , Talc, calcium carbonate and the like. The safflower petal powder and / or extract, which is the pharmaceutical composition, is known in the field of pharmaceutical or food production, together with pharmaceutically acceptable salts, excipients, preservatives, coloring agents, flavoring agents, and the like. Depending on the method, it can be used in various forms suitable for oral administration, for example, powder, liquid, granule, tablet, capsule and the like. The solution may be either a solution or a suspension, and examples of the carrier include water, hydrogenated castor oil, glycerin, propylene glycol, ethanol, fatty oil, ethylene glycol, polyethylene glycol, sorbitol and the like.

  Examples of the preventive and therapeutic agent for diabetes according to the present invention will be described in detail below.

(Preparation Example 1: Preparation of safflower petal powder (1))
200 g of dried safflower petals from China are crushed and freeze-dried, and then the freeze-dried product is made into a powder of about 200 mesh using a mortar. The safflower petal powder (1) thus prepared is used in a postprandial blood glucose elevation inhibitory test using rats.

(Preparation Example 2: Preparation of safflower petal water extract (2))
200g dried safflower petals from China were crushed and cold soaked overnight in 2L distilled water. The obtained water extract was treated by lyophilization to obtain 87 g of a powder lyophilized product (2). As a result of HPLC analysis, this powdery lyophilized product is a mixture containing yellow pigments saflomin A, safflower yellow B, and the like. The freeze-dried product (2) of this water extract is used for a postprandial blood glucose increase inhibitory test using rats.

(Preparation Example 3: Preparation of water-insoluble freeze-dried product (3) of safflower petals)
In accordance with a known method of crushing 200 g of dried safflower petals from China and preparing the red pigment carthamin from safflower petals that has been used since ancient times, suspend the dried safflower petals into sodium bicarbonate water, Use and stir well. Thereafter, the mixer-treated product is allowed to stand on ice for 6 hours. Subsequently, the mixer-treated product is filtered to obtain a filtrate. Citric acid is added to the filtrate to make H4.5, and the mixture is allowed to stand overnight on ice. The deposited precipitate is separated by filtration. Subsequently, the precipitate was suspended in water and lyophilized to obtain 5 g of lyophilized product (3). The freeze-dried product (3) is a mixture containing the red pigment cartamine as a result of HPLC analysis. This freeze-dried product (3) is used for a postprandial blood glucose increase inhibitory test using rats.

(Preparation Example 4: Preparation of safflower petal water-insoluble freeze-dried product (4))
200 g of dried petals of Chinese safflower in Example 2 were subjected to a cold immersion overnight in 2 L of distilled water, and the water-insoluble fraction was separated by filtration using a glass filter. The obtained water-insoluble matter is suspended in sodium bicarbonate water according to a known method for preparing a red pigment carthamin from safflower petals, which has been performed since ancient times, and sufficiently stirred using a mixer or the like. Thereafter, the mixer-treated product is allowed to stand on ice for 6 hours. Subsequently, the mixer-treated product is filtered to obtain a filtrate. Citric acid is added to the filtrate to adjust the pH to 4.5 and the mixture is allowed to stand overnight on ice. The deposited precipitate is separated by filtration. Subsequently, the precipitate was suspended in water and lyophilized to obtain 2.6 g of lyophilized product (4). The freeze-dried product (4) is a mixture containing the red pigment cartamine as a result of HPLC analysis. This freeze-dried product (4) is used in a postprandial blood glucose increase inhibitory test using rats.

(Preparation Example 5: Preparation of yellow pigment saflomin A)
A raw safflower petal 10 kg was immersed in methanol for 1 week, and the extract obtained by filtering the petals was concentrated under reduced pressure. The obtained residue was washed with ethyl acetate, and the residue was separated by silica gel column chromatography using the upper layer of n-butanol: water: acetic acid = 4: 5: 1 to obtain 40 g of crude saflomin A. This was further purified by Sephadex LH-20 column chromatography using a methanol-water mixed solvent as a developing solution to obtain 30 g of purified saflomin A. The purified saflomin A thus prepared is used in a postprandial blood glucose elevation inhibitory test using rats.

(Preparation Example 6: Preparation of red dye carthamin)
210 g of dried safflower petals were thoroughly washed with water, 1.3 L of 5% aqueous sodium carbonate solution was added, and the mixture was allowed to stand for 6 hours under ice cooling. The petal was filtered and citric acid was added little by little to the filtrate to adjust the pH to 4.5. After ice-cooling overnight, the mixture was centrifuged, and the resulting precipitate was lyophilized to obtain 5 g of red crystals. The crude carthamin obtained here was powdered and added with acetone and allowed to stand overnight, and the residue was recrystallized with acetone-water to obtain 200 mg of purified cartamine. The purified cartamine prepared as described above is used in a postprandial blood glucose increase inhibitory test using rats.

(Postprandial blood glucose elevation inhibitory effect test)
Crj: CD (SD) IGS male rats purchased from Nippon Charles River Co., Ltd. were used. Three mice were used in the experiment, and the group was divided into a control group and an extract administration group so that the average body weight and the average blood glucose level showed approximate values. For the extract administration group, the water-insoluble lyophilized product (3) of safflower petals prepared in Example 3 and the water-insoluble lyophilized product (4) of safflower petals prepared in Example 4 were subjected to the test. Rats were fasted for 18 hours, blood was collected from the jugular vein, and blood glucose levels were measured with SYNCHRON CX3Δ (manufactured by BECKMAN). Subsequently, the water-insoluble lyophilized product (3) and the water-insoluble lyophilized product (4) were suspended in purified water and gavaged at the doses shown in Table 1 as lyophilized products. kg was administered by oral gavage. Blood samples were collected from the jugular vein 30 minutes, 60 minutes and 120 minutes after glucose administration, and blood glucose levels were measured with SYNCHRON CX3Δ (manufactured by BECKMAN).
The blood glucose level good suppression rate was calculated from the following formula.
Glucose level rise inhibition rate (%) = {1-[(extract administration group blood glucose level-extract administration group blood glucose level before start of extract administration) / (control group blood glucose level-control group blood glucose level before start of extract administration) Value)]} x 100
The obtained results are shown in Table 1.

表１から、本発明の抽出物群は、優れた食後血糖上昇抑制作用を示すことがわかる。

From Table 1, it can be seen that the extract group of the present invention exhibits an excellent postprandial blood glucose increase inhibitory action.

(Postprandial blood glucose elevation inhibitory effect test)
Crj: CD (SD) IGS male rats purchased from Nippon Charles River Co., Ltd. were used.
Three mice were used in the experiment, and the group was divided into a control group and an extract administration group so that the average body weight and the average blood glucose level showed approximate values. For the extract administration group, the water-insoluble lyophilized product (4) of safflower petals prepared in Example 4 and the water extract (2) of safflower petals prepared in Example 2 were subjected to the test. Rats were fasted for 18 hours, blood was collected from the jugular vein, and blood glucose levels were measured with SYNCHRON CX3Δ (manufactured by BECKMAN). Subsequently, a sample prepared by dissolving a water-insoluble freeze-dried product of safflower petals (4) in a 20% hydrogenated castor oil aqueous solution and a sample obtained by dissolving a water extract of safflower petals (2) in purified water were prepared. As a result, gavage was administered at the dose shown in Table 2 and 15 minutes later, glucose 2.5 g / kg was administered by gavage. Blood was collected from the jugular vein 30 minutes, 60 minutes and 120 minutes after glucose administration, and the blood glucose level was measured with SYNCHRON CX3Δ (manufactured by BECKMAN).
The blood glucose level increase inhibition rate was calculated from the following formula.
Glucose level rise inhibition rate (%) = {1-[(extract administration group blood glucose level-extract administration group blood glucose level before start of extract administration) / (control group blood glucose level-control group blood glucose level before start of extract administration) Value)]} x 100
The obtained results are shown in Table 2.

表２から、本発明の抽出物群は優れた食後血糖上昇抑制作用を示すことがわかる。

From Table 2, it can be seen that the extract group of the present invention exhibits an excellent postprandial blood glucose increase inhibitory action.

(Postprandial blood glucose elevation inhibitory effect test)
Crj: CD (SD) IGS male rats purchased from Nippon Charles River Co., Ltd. were used. Three animals were used in the experiment, and the group was divided into a control group and a safflower petal / pigment-administered group so that the average body weight and the average blood glucose level showed approximate values. The safflower petal / pigment administration group was subjected to the test with the yellow pigment saflomin A prepared in Example 5 and the red pigment cartamine prepared in Example 6.
Rats were fasted for 18 hours, blood was collected from the jugular vein, and blood glucose levels were measured with SYNCHRON CX3Δ (manufactured by BECKMAN). Subsequently, a sample in which the red pigment cartamine was suspended in 20% hydrogenated castor oil aqueous solution and a sample in which the yellow pigment safurin A was dissolved in purified water were prepared and each of them was orally administered by gavage at the doses shown in Table 3 as lyophilized products. After 2.5 minutes, glucose 2.5 g / kg was orally administered by gavage. Blood was collected from the jugular vein 30 minutes, 60 minutes and 120 minutes after glucose administration, and the blood glucose level was measured with SYNCHRON CX3Δ (manufactured by BECKMAN).
The blood glucose level increase inhibition rate was calculated from the following formula.
Glucose level rise inhibition rate (%) = {1-[(extract administration group blood glucose level-extract administration group blood glucose level before start of extract administration) / (control group blood glucose level-control group blood glucose level before start of extract administration) Value)]} x 100

表３から、本発明の抽出物群は優れた食後血糖上昇抑制作用を示すことがわかる。The obtained results are shown in Table 3.

From Table 3, it can be seen that the extract group of the present invention exhibits an excellent postprandial blood glucose increase inhibitory action.

Examples of preparations and foods are shown below.

(Manufacture of drinks)
[Prescription] Composition and blending amount Safflower petal water extract (Example 2) 1.0 g
Calcium lactate pentahydrate 46.1g
DL sodium tartrate 10mg
Succinic acid 1mL
Liquid sugar 80g
Citric acid 1.2g
Vitamin C 1.0g
Fragrance 1mL
Potassium chloride 0.1g
Magnesium sulfate 50mg
[Production method]
The above composition was dissolved in 800 mL of distilled water, further distilled water was added to make a total volume of 1000 mL, sterilized with a 0.22 μm sterilization filter, and 100 mL each was aseptically filled into brown bottles to prepare in Example 2. A drink containing 100 mg of the active ingredient of the antidiabetic agent of the present invention was prepared.

(Manufacture of chewable tablets)
[Prescription] Composition and blending amount Water extract of safflower petals (Example 2) 10 parts by weight Maltocyclodextrin 14 parts by weight Corn starch 11 parts by weight Glucose 38 parts by weight Gelatin 5 parts by weight Fragrance 0.2 parts by weight Hakka 3.8 parts by weight Part time 2 parts by weight Calcium hydrogen phosphate 15 parts by weight Sucrose fatty acid ester 1 part by weight [Production method]
In the above composition, materials excluding fragrance, mint, thyme and sucrose fatty acid ester are mixed well in a mill, and then distilled water is added to knead to an appropriate viscosity that can be molded. To this, mint, thyme, and sucrose fatty acid ester are added and further kneaded, and finally a fragrance is added to produce granules by extrusion granulation. The granules were dried at 40 ° C. and then molded using a tableting machine to produce chewable tablets.

(Manufacture of baked goods)
[Prescription]
Water-insoluble lyophilized product of safflower petal (Example 3) 1 part by weight flour 50 parts by weight corn starch 20 parts by weight fructose 13 parts by weight butter 5 parts by weight sodium chloride 0.5 parts by weight sodium hydrogen carbonate 0.5 parts by weight water 10 parts by weight Part [production method]
In the above composition, mix well to make dough. This was formed into an appropriate shape and baked in an oven to make a baked confectionery.

(Manufacture of gelatin capsules)
[Prescription]
Gelatin 70 parts by weight Glycerin 22.9 parts by weight Methyl paraoxybenzoate 0.15 parts by weight Propyl paraoxybenzoate 0.51 parts by weight Water 6.44 parts by weight [Production method]
A gelatin capsule was produced by filling 50 mg of a water-insoluble freeze-dried safflower petal (Example 4) into a gelatin capsule skin (oval shape, weight 150 mg) having the above composition.

(Manufacture of tablets)
[Prescription] Composition and blending amount Safflower petal water extract (Example 2) 20 parts by weight Crystalline cellulose 10 parts by weight Sucrose ester 5 parts by weight Silicon dioxide 2 parts by weight Eggshell calcium 63 parts by weight [Production method]
The above composition was mixed and stirred to adjust uniformly, and a tablet was produced using a tableting machine.

The safflower petal and / or extract thereof of the present invention can be effectively used as a preventive / therapeutic agent for diabetes or a health food.

Claims (9)

An anti-diabetic composition comprising petals of an Asteraceae plant safflower ( Carthamus tinctorius L. ) and / or an extract thereof as an active ingredient. The antidiabetic composition according to claim 1, wherein the petals are in powder form. The antidiabetic composition according to claim 1 or 2, wherein the extract is in the form of an extract or a lyophilized product. The antidiabetic according to claim 1 or 3, which is prepared using an alcohol having 1 to 8 carbon atoms, water, a mixed solvent of water and the alcohol, an extraction solvent selected from the group consisting of ethyl acetate and acetone. Composition. An antidiabetic composition comprising, as an active ingredient, a red pigment, carthamin, contained in petals of a safflower plant ( Carthamus tinctorius L. ). An anti-diabetic composition comprising, as an active ingredient, a yellow pigment safflower yellows contained in petals of a safflower plant ( Carthamus tinctorius L. ). An anti-diabetic composition comprising, as an active ingredient, a yellow pigment, saflomin A contained in petals of a safflower plant ( Carthamus tinctorius L. ). A precipitate obtained by treating a petrolatum ( Carthamus tinctorius L. ) petal or its water-insoluble matter with a basic condition in an aqueous medium to remove the insoluble part, and further treating it with an acidic condition in an aqueous medium. An anti-diabetic composition containing a product as an active ingredient. It is a diabetes preventive and / or therapeutic agent, The composition for antidiabetics in any one of Claims 1-8.