JP3362847B2 - ペプチドリガーゼ活性を有するセリンプロテアーゼ変異体 - Google Patents

ペプチドリガーゼ活性を有するセリンプロテアーゼ変異体

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Publication number
JP3362847B2
JP3362847B2 JP51465591A JP51465591A JP3362847B2 JP 3362847 B2 JP3362847 B2 JP 3362847B2 JP 51465591 A JP51465591 A JP 51465591A JP 51465591 A JP51465591 A JP 51465591A JP 3362847 B2 JP3362847 B2 JP 3362847B2
Authority
JP
Japan
Prior art keywords
group
ligation
subtilisin
serine protease
amino acid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
JP51465591A
Other languages
English (en)
Japanese (ja)
Other versions
JPH06500019A (ja
Inventor
ラルス アブラムセン
ジョン バーニア
ジェイムズ エイ ウェルス
Original Assignee
ジェネンテック インコーポレイテッド
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Filing date
Publication date
Application filed by ジェネンテック インコーポレイテッド filed Critical ジェネンテック インコーポレイテッド
Publication of JPH06500019A publication Critical patent/JPH06500019A/ja
Application granted granted Critical
Publication of JP3362847B2 publication Critical patent/JP3362847B2/ja
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/61—Growth hormone [GH], i.e. somatotropin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Endocrinology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
JP51465591A 1990-08-09 1991-08-06 ペプチドリガーゼ活性を有するセリンプロテアーゼ変異体 Expired - Lifetime JP3362847B2 (ja)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US56602690A 1990-08-09 1990-08-09
US566,026 1990-08-09

Publications (2)

Publication Number Publication Date
JPH06500019A JPH06500019A (ja) 1994-01-06
JP3362847B2 true JP3362847B2 (ja) 2003-01-07

Family

ID=24261145

Family Applications (1)

Application Number Title Priority Date Filing Date
JP51465591A Expired - Lifetime JP3362847B2 (ja) 1990-08-09 1991-08-06 ペプチドリガーゼ活性を有するセリンプロテアーゼ変異体

Country Status (8)

Country Link
US (2) US5403737A (enExample)
EP (1) EP0542931B1 (enExample)
JP (1) JP3362847B2 (enExample)
AT (1) ATE187495T1 (enExample)
CA (1) CA2087342C (enExample)
DE (1) DE69131834T2 (enExample)
DK (1) DK0542931T3 (enExample)
WO (1) WO1992002615A1 (enExample)

Families Citing this family (39)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5629173A (en) * 1990-08-09 1997-05-13 Genentech, Inc. Methods of use of serine protease variants having peptide ligase activity
WO1994018329A2 (en) * 1993-02-04 1994-08-18 Genentech, Inc. Serine protease variants having peptide ligase activity
US6436690B1 (en) * 1993-09-15 2002-08-20 The Procter & Gamble Company BPN′ variants having decreased adsorption and increased hydrolysis wherein one or more loop regions are substituted
US6440717B1 (en) * 1993-09-15 2002-08-27 The Procter & Gamble Company BPN′ variants having decreased adsorption and increased hydrolysis
US6187579B1 (en) 1993-10-28 2001-02-13 Carlsberg A/S Customized proteases
US6599730B1 (en) * 1994-05-02 2003-07-29 Procter & Gamble Company Subtilisin 309 variants having decreased adsorption and increased hydrolysis
US5780285A (en) * 1995-03-03 1998-07-14 Genentech, Inc. Subtilisin variants capable of cleaving substrates containing dibasic residues
US5837516A (en) * 1995-03-03 1998-11-17 Genentech, Inc. Subtilisin variants capable of cleaving substrates containing basic residues
US6455295B1 (en) * 1995-03-08 2002-09-24 The Procter & Gamble Company Subtilisin Carlsberg variants having decreased adsorption and increased hydrolysis
US6475765B1 (en) * 1995-03-09 2002-11-05 Procter & Gamble Company Subtilisin DY variants having decreased adsorption and increased hydrolysis
IL117350A0 (en) * 1995-03-09 1996-07-23 Procter & Gamble Proteinase k variants having decreased adsorption and increased hydrolysis
US5985627A (en) * 1997-02-28 1999-11-16 Carlsberg Laboratory Modified carboxypeptidase
US5858758A (en) * 1997-05-07 1999-01-12 Incyte Pharmaceuticals, Inc. Human serine protease precursor
ES2368718T3 (es) * 1997-10-23 2011-11-21 Danisco Us Inc. Variantes de subtilisina con múltiples sustituciones.
KR20010040398A (ko) * 1998-01-23 2001-05-15 마가렛 에이.혼 화학적으로 개질된 돌연변이 효소 및 이를 제조하는 방법및 스크리닝하는 방법
CA2321169C (en) * 1998-01-23 2011-08-02 Genencor International, Inc. Modified enzymes and their use for peptide synthesis
AU749383B2 (en) 1998-07-02 2002-06-27 Genencor International, Inc. Chemically modified proteins with a carbohydrate moiety
WO2000028007A2 (en) 1998-11-10 2000-05-18 Genencor International, Inc. Chemically modified mutant serine hydrolases
CA2353789C (en) * 1998-12-21 2012-02-14 Genencor International, Inc. Chemically modified enzymes with multiple charged variants
MXPA01010896A (es) * 1999-04-28 2003-06-24 Genencor Int Antagonistas cataliticos especificamente dirigidos al objetivo y usos de los mismos.
US6627744B2 (en) 1999-07-02 2003-09-30 Genencor International, Inc. Synthesis of glycodendrimer reagents
US6541234B1 (en) * 2000-09-11 2003-04-01 University Of Maryland Biotechnology Institute Calcium free subtilisin mutants
US6541235B1 (en) * 2000-09-29 2003-04-01 University Of Maryland Biotechnology Institute Calcium free subtilisin mutants
DE10049673A1 (de) 2000-10-07 2002-04-25 Univ Leipzig Verfahren zur Herstellung von Peptiden, Peptidmimetika und Proteinen
US20040097720A1 (en) * 2001-04-02 2004-05-20 Bott Richard R. Use of glycodendrimer reagents for inhibiting adhesion by microorganisms
US6713529B2 (en) * 2001-08-10 2004-03-30 Illinois Tool Works, Inc. Phase-change ink composition
US20040241780A1 (en) * 2001-08-24 2004-12-02 Jones J. Bryan Chemically modified mutant enzymes and methods for producing them, and screening them for amidase and /or esterase activity
US20080221027A1 (en) * 2007-03-09 2008-09-11 The Regents Of The University Of California Composition and Methods for the Treatment of Duchene Muscular Dystrophy
EP2191828B1 (en) * 2007-09-05 2016-08-10 Pola Pharma Inc. Antifungal pharmaceutical composition
US10138268B2 (en) 2014-10-10 2018-11-27 Enzypep B.V. Peptide fragment condensation and cyclisation using a subtilisin variant with improved synthesis over hydrolysis ratio
US10752931B2 (en) * 2015-07-09 2020-08-25 Enzypep B.V. Designing an enzymatic peptide fragment condensation strategy
EP3299085A1 (en) 2016-09-26 2018-03-28 Enzypep B.V. Peptide coupling molecule
EP3404036A1 (en) 2017-05-19 2018-11-21 Enzypep B.V. Subtilisin variants having a mutation in the s2 or s2' pocket
US10858414B2 (en) 2018-03-09 2020-12-08 Enzypep B.V. Chemo-enzymatic synthesis of semaglutide, liraglutide and GLP-1
CN119060201A (zh) 2018-03-09 2024-12-03 费森尤斯卡比有限公司 利拉鲁肽、索马鲁肽和glp-1的化学酶促合成
WO2022171667A1 (en) 2021-02-12 2022-08-18 Fresenius Kabi Ipsum S.R.L. Subtilisin variants and their use
EP4122944A1 (en) 2021-07-21 2023-01-25 EnzyTag B.V. Enzymatic synthesis of cyclic peptides
CA3274855A1 (en) 2022-12-19 2024-06-27 Fresenius Kabi Ipsum S.R.L. THERMOSABLE VARIANTS OF SUBTILISINE AND THEIR USE
WO2025058511A1 (en) * 2023-09-12 2025-03-20 Endoprotease Services B.V. Acyltransferase-catalysed modification of peptides

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4760025A (en) * 1984-05-29 1988-07-26 Genencor, Inc. Modified enzymes and methods for making same
US5155033A (en) * 1989-01-06 1992-10-13 Genencor, Inc. Subtilisins modified at position 225 resulting in a shift in catalytic activity
IE65767B1 (en) * 1986-04-30 1995-11-15 Genencor Int Non-human carbonyl hydrolase mutants DNA sequences and vectors encoding same and hosts transformed with said vectors

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
Ｂｉｏｃｈｅｍｉｓｔｒｙ，１９８５，Ｖｏｌ．２４，Ｎｏ．７，ｐ．１７９８−１８０６
Ｊ．ｏｆ Ｍｏｌｅｃｕｌａｒ Ｌｉｑｕｉｄｓ，１９８９，Ｖｏｌ．４２，ｐ．１９５−２１２
Ｊ．ｏｆ ｔｈｅ Ａｍｅｒｉｃａｎ Ｃｈｅｍｉｃａｌ Ｓｉｃｉｅｔｙ，１９８７，Ｖｏｌ．１０９，Ｎｏ

Also Published As

Publication number Publication date
CA2087342A1 (en) 1992-02-10
DE69131834D1 (de) 2000-01-13
DK0542931T3 (da) 2000-05-22
ATE187495T1 (de) 1999-12-15
WO1992002615A1 (en) 1992-02-20
US5403737A (en) 1995-04-04
EP0542931A4 (enExample) 1994-04-13
US5763256A (en) 1998-06-09
DE69131834T2 (de) 2000-05-31
CA2087342C (en) 2003-12-23
EP0542931A1 (en) 1993-05-26
EP0542931B1 (en) 1999-12-08
JPH06500019A (ja) 1994-01-06

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