DK0542931T3 - Serinproteasevarianter med peptidligaseaktivitet - Google Patents
Serinproteasevarianter med peptidligaseaktivitetInfo
- Publication number
- DK0542931T3 DK0542931T3 DK92903763T DK92903763T DK0542931T3 DK 0542931 T3 DK0542931 T3 DK 0542931T3 DK 92903763 T DK92903763 T DK 92903763T DK 92903763 T DK92903763 T DK 92903763T DK 0542931 T3 DK0542931 T3 DK 0542931T3
- Authority
- DK
- Denmark
- Prior art keywords
- protease variants
- serine protease
- ligase activity
- ligation
- peptide ligase
- Prior art date
Links
- 102000012479 Serine Proteases Human genes 0.000 title abstract 4
- 108010022999 Serine Proteases Proteins 0.000 title abstract 4
- 102000003960 Ligases Human genes 0.000 title abstract 2
- 108090000364 Ligases Proteins 0.000 title abstract 2
- 108090000765 processed proteins & peptides Proteins 0.000 title abstract 2
- 239000000758 substrate Substances 0.000 abstract 2
- 108091005804 Peptidases Proteins 0.000 abstract 1
- 239000004365 Protease Substances 0.000 abstract 1
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 abstract 1
- 239000002243 precursor Substances 0.000 abstract 1
- 239000000126 substance Substances 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
- C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/61—Growth hormone [GH], i.e. somatotropin
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/22—Ribonucleases RNAses, DNAses
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Molecular Biology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Medicinal Chemistry (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Endocrinology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Toxicology (AREA)
- Gastroenterology & Hepatology (AREA)
- Biophysics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US56602690A | 1990-08-09 | 1990-08-09 | |
PCT/US1991/005480 WO1992002615A1 (en) | 1990-08-09 | 1991-08-06 | Serine protease variants having peptide ligase activity |
Publications (1)
Publication Number | Publication Date |
---|---|
DK0542931T3 true DK0542931T3 (da) | 2000-05-22 |
Family
ID=24261145
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DK92903763T DK0542931T3 (da) | 1990-08-09 | 1991-08-06 | Serinproteasevarianter med peptidligaseaktivitet |
Country Status (8)
Country | Link |
---|---|
US (2) | US5403737A (da) |
EP (1) | EP0542931B1 (da) |
JP (1) | JP3362847B2 (da) |
AT (1) | ATE187495T1 (da) |
CA (1) | CA2087342C (da) |
DE (1) | DE69131834T2 (da) |
DK (1) | DK0542931T3 (da) |
WO (1) | WO1992002615A1 (da) |
Families Citing this family (38)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5629173A (en) * | 1990-08-09 | 1997-05-13 | Genentech, Inc. | Methods of use of serine protease variants having peptide ligase activity |
WO1994018329A2 (en) * | 1993-02-04 | 1994-08-18 | Genentech, Inc. | Serine protease variants having peptide ligase activity |
US6436690B1 (en) * | 1993-09-15 | 2002-08-20 | The Procter & Gamble Company | BPN′ variants having decreased adsorption and increased hydrolysis wherein one or more loop regions are substituted |
US6440717B1 (en) * | 1993-09-15 | 2002-08-27 | The Procter & Gamble Company | BPN′ variants having decreased adsorption and increased hydrolysis |
US6187579B1 (en) * | 1993-10-28 | 2001-02-13 | Carlsberg A/S | Customized proteases |
US6599730B1 (en) * | 1994-05-02 | 2003-07-29 | Procter & Gamble Company | Subtilisin 309 variants having decreased adsorption and increased hydrolysis |
US5837516A (en) * | 1995-03-03 | 1998-11-17 | Genentech, Inc. | Subtilisin variants capable of cleaving substrates containing basic residues |
US5780285A (en) * | 1995-03-03 | 1998-07-14 | Genentech, Inc. | Subtilisin variants capable of cleaving substrates containing dibasic residues |
US6455295B1 (en) * | 1995-03-08 | 2002-09-24 | The Procter & Gamble Company | Subtilisin Carlsberg variants having decreased adsorption and increased hydrolysis |
IL117350A0 (en) * | 1995-03-09 | 1996-07-23 | Procter & Gamble | Proteinase k variants having decreased adsorption and increased hydrolysis |
US6475765B1 (en) * | 1995-03-09 | 2002-11-05 | Procter & Gamble Company | Subtilisin DY variants having decreased adsorption and increased hydrolysis |
US5985627A (en) * | 1997-02-28 | 1999-11-16 | Carlsberg Laboratory | Modified carboxypeptidase |
US5858758A (en) * | 1997-05-07 | 1999-01-12 | Incyte Pharmaceuticals, Inc. | Human serine protease precursor |
ES2368718T3 (es) * | 1997-10-23 | 2011-11-21 | Danisco Us Inc. | Variantes de subtilisina con múltiples sustituciones. |
JP4475371B2 (ja) | 1998-01-23 | 2010-06-09 | ジェネンコア インターナショナル インコーポレーテッド | 化学的に修飾された突然変異体酵素およびその産生方法とスクリーニング方法 |
US6395532B1 (en) | 1998-01-23 | 2002-05-28 | Genencor International, Inc. | Modified enzymes and their use for peptide synthesis |
ATE407941T1 (de) * | 1998-07-02 | 2008-09-15 | Univ Toronto | Mit einer kohlenhydratgruppe chemisch modifizierte proteine |
AU772427B2 (en) * | 1998-11-10 | 2004-04-29 | Genencor International, Inc. | Chemically modified mutant serine hydrolases show improved catalytic activity and chiral selectivity |
DE69941223D1 (de) | 1998-12-21 | 2009-09-17 | Billig Fritz | Chemisch modifizierte enzymen mit mehrfachgeladenen varianten |
JP5180417B2 (ja) | 1999-04-28 | 2013-04-10 | ジェネンコー・インターナショナル・インク | 特異的に標的化された触媒性アンタゴニストおよびその使用 |
US6627744B2 (en) * | 1999-07-02 | 2003-09-30 | Genencor International, Inc. | Synthesis of glycodendrimer reagents |
US6541234B1 (en) * | 2000-09-11 | 2003-04-01 | University Of Maryland Biotechnology Institute | Calcium free subtilisin mutants |
US6541235B1 (en) * | 2000-09-29 | 2003-04-01 | University Of Maryland Biotechnology Institute | Calcium free subtilisin mutants |
DE10049673A1 (de) | 2000-10-07 | 2002-04-25 | Univ Leipzig | Verfahren zur Herstellung von Peptiden, Peptidmimetika und Proteinen |
US20040097720A1 (en) * | 2001-04-02 | 2004-05-20 | Bott Richard R. | Use of glycodendrimer reagents for inhibiting adhesion by microorganisms |
US6713529B2 (en) * | 2001-08-10 | 2004-03-30 | Illinois Tool Works, Inc. | Phase-change ink composition |
US20040241780A1 (en) * | 2001-08-24 | 2004-12-02 | Jones J. Bryan | Chemically modified mutant enzymes and methods for producing them, and screening them for amidase and /or esterase activity |
US20080221027A1 (en) * | 2007-03-09 | 2008-09-11 | The Regents Of The University Of California | Composition and Methods for the Treatment of Duchene Muscular Dystrophy |
EP2191828B1 (en) * | 2007-09-05 | 2016-08-10 | Pola Pharma Inc. | Antifungal pharmaceutical composition |
JP7017409B2 (ja) * | 2014-10-10 | 2022-02-08 | エンザイペプ ビー.ブイ. | 加水分解に対する合成の比が改善したスブチリシン変異体を使用するペプチド断片縮合及び環化 |
WO2017007324A1 (en) | 2015-07-09 | 2017-01-12 | Enzypep B.V. | Designing an enzymatic peptide fragment condensation strategy |
EP3299085A1 (en) | 2016-09-26 | 2018-03-28 | Enzypep B.V. | Peptide coupling molecule |
EP3404036A1 (en) | 2017-05-19 | 2018-11-21 | Enzypep B.V. | Subtilisin variants having a mutation in the s2 or s2' pocket |
CA3093221A1 (en) | 2018-03-09 | 2019-09-12 | Enzypep B.V. | Chemo-enzymatic synthesis of liraglutide, semaglutide and glp-1 |
US10858414B2 (en) | 2018-03-09 | 2020-12-08 | Enzypep B.V. | Chemo-enzymatic synthesis of semaglutide, liraglutide and GLP-1 |
KR20230144058A (ko) | 2021-02-12 | 2023-10-13 | 프레제니우스 카비 입섬 에스. 알. 엘. | 서브틸리신 변이체 및 그의 용도 |
EP4122944A1 (en) | 2021-07-21 | 2023-01-25 | EnzyTag B.V. | Enzymatic synthesis of cyclic peptides |
WO2024133313A1 (en) | 2022-12-19 | 2024-06-27 | Enzypep B.V. | Thermostable subtilisin variants and their use |
Family Cites Families (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4760025A (en) * | 1984-05-29 | 1988-07-26 | Genencor, Inc. | Modified enzymes and methods for making same |
US5155033A (en) * | 1989-01-06 | 1992-10-13 | Genencor, Inc. | Subtilisins modified at position 225 resulting in a shift in catalytic activity |
ATE116365T1 (de) * | 1986-04-30 | 1995-01-15 | Genencor Int | Mutante einer nicht menschlichen carbonyl- hydrolase, für diese kodierende dns-sequenzen und vektoren und durch diese vektoren transformierte wirte. |
-
1991
- 1991-08-06 US US07/969,154 patent/US5403737A/en not_active Expired - Lifetime
- 1991-08-06 WO PCT/US1991/005480 patent/WO1992002615A1/en active IP Right Grant
- 1991-08-06 EP EP92903763A patent/EP0542931B1/en not_active Expired - Lifetime
- 1991-08-06 DE DE69131834T patent/DE69131834T2/de not_active Expired - Lifetime
- 1991-08-06 AT AT92903763T patent/ATE187495T1/de not_active IP Right Cessation
- 1991-08-06 CA CA002087342A patent/CA2087342C/en not_active Expired - Lifetime
- 1991-08-06 JP JP51465591A patent/JP3362847B2/ja not_active Expired - Lifetime
- 1991-08-06 DK DK92903763T patent/DK0542931T3/da active
-
1994
- 1994-06-08 US US08/257,467 patent/US5763256A/en not_active Expired - Lifetime
Also Published As
Publication number | Publication date |
---|---|
CA2087342A1 (en) | 1992-02-10 |
EP0542931A1 (en) | 1993-05-26 |
US5763256A (en) | 1998-06-09 |
JP3362847B2 (ja) | 2003-01-07 |
ATE187495T1 (de) | 1999-12-15 |
WO1992002615A1 (en) | 1992-02-20 |
CA2087342C (en) | 2003-12-23 |
JPH06500019A (ja) | 1994-01-06 |
EP0542931A4 (da) | 1994-04-13 |
EP0542931B1 (en) | 1999-12-08 |
DE69131834D1 (de) | 2000-01-13 |
DE69131834T2 (de) | 2000-05-31 |
US5403737A (en) | 1995-04-04 |
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