JP3167478B2 - Liquid separation device - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a liquid separating apparatus for taking out each layer of liquid from a flexible container in which liquid separated into a plurality of layers is sealed, such as a blood bag.

[0002]

2. Description of the Related Art In recent years, instead of whole blood transfusion, component transfusion for transfusing only necessary components in blood to a patient or the like has been performed, or only plasma has been collected from blood for preparing a plasma preparation. Has also been done. In order to obtain such blood for component transfusion and plasma preparation, a flexible blood bag called a so-called multi-bag has been conventionally used. The blood bag includes a blood collection bag (so-called parent bag) for storing blood collected from a donor, and one or a plurality of separation bags connected to the parent bag via a fluid extraction tube (hereinafter simply “tube”). (A so-called child bag), and blood components in the parent bag separated by centrifugation are taken out for each component and taken into the child bag.

When a bag for blood collection is centrifuged, the blood becomes a plasma layer, an erythrocyte layer and an intermediate layer (a buffy coat layer which is a buffy coat layer containing platelets, leukocytes or fats, and further various bacteria, etc.). Is separated for each component, but in order to use the separated components later, it is necessary to separate and collect the components from the blood collection bag into each separation bag so that the components do not mix with each other. . In order to perform such fractional sampling, a liquid separating device is required in addition to the blood bag.
10 to 10.

In a blood bag 100A shown in FIG. 6, a tube is attached to an upper portion of a parent bag 101 that stores collected blood. Tubes t1 to t3 are connected to this tube via two T-shaped branch pipes. At the tip of each tube t1 to t3, a child bag 102a containing a red blood cell preserving solution and two empty child bags 102b, 10
2c is connected. To separate each component from the parent bag 101, first, the plasma layer S is centrifuged.
1. The abdominal surface of the parent bag 101 containing the blood separated into the three layers of the red blood cell layer S2 and the intermediate layer S3 is pressurized so that the plasma component forming the upper layer S1 is supplied to the child bag 102b via the tube t2. Transfer to When the transfer of the plasma components is completed, a small amount of the plasma components remaining in the parent bag 101, the components of the intermediate layer, that is, the buffy coat layer, and a part of the red blood cell components of the lower layer are transferred to the child bag 102c via the tube t3. Transfer. Thus, the parent bag 101
After the intermediate layer is removed from the inside, the red blood cell preservation solution contained in the child bag 102a is transferred into the parent bag 101 via the tube t1. The blood bag 100A that separates blood components in this way is practical because the components can be collected from one direction of the parent bag 101 (in this example, from above).

FIG. 7 shows a blood collection bag 10.
Tubes t1 and t2 are provided above and below 0B. The parent bag 1 is separated into the three layers by centrifugation.
01 is attached to a liquid separation device, and pressurized in the same manner as described above to remove the plasma layer S1 from the upper tube t1.
The red blood cell layer S2 is taken out from the lower tube t2 to the child bag 102, and the intermediate layer S3 is left in the parent bag 101 (see Japanese Patent Publication No. 63-20,144). The blood collection bag 100B takes out desired components vertically from the centrifuged state, so that the intermediate layer can be efficiently separated and collected from the blood. Note that reference numeral “103” in the drawing denotes a blood collection needle.

A bag 100C shown in FIG.
A partition wall 104 extending to the bottom of the bag is formed by heat-sealing the front and back surfaces of the front and rear surfaces of the bag 01, thereby independently forming an upper take-out passage 105 in the bag. Then, the centrifuged parent bag 101 is attached to the liquid separator and pressurized to take out the plasma layer S1 from the first tube t1, the red blood cell layer S2 from the second tube t2, and the intermediate layer S3. Bag 101
(See European Patent Publication No. 0484751A1). This blood bag is
It is practical because each component can be taken out from above.

On the other hand, in a liquid separating apparatus using such a blood bag, a lower pressure bias type as shown in FIG. 9 (for example, Japanese Patent Publication No. -17,585)
And a parallel pressing method as shown in FIG. 10 (see, for example, Japanese Patent Application Laid-Open No. 55-155,652). The former is a method in which the parent bag 101 is hung on a hook provided on the separation stand 106 and the abdominal surface of the bag is pressed by a pressing plate 107 whose lower end is rotatably supported. In this method, a pressure plate 108 is provided, and the abdominal surface of the bag is pressed in this parallel state. These include automatic pressurization and manual pressurization. In the automatic pressurization, which has been widely used in recent years, any of these methods applies the pressure while detecting the position of the intermediate layer by an interface sensor (not shown). A method of pressing the parent bag with the pressure plate 107 or 108 is adopted.

[0008]

However, as in the bag 100A shown in FIG.
What is taken out to the c is the middle layer S3 on the inner wall of the parent bag 10.
And the timing of removing the intermediate layer S3 is difficult, and there is a problem in controllability. The bag 10 shown in FIG.
In the case where the tubes t1 and t2 are provided above and below the bag, such as 0B, the component take-out ports are vertically arranged, so that handling of the tubes becomes inconvenient when the bag is attached to the liquid separation device.

In the case where a partition wall 104 extending to the bottom of the bag is formed as in a bag 100C shown in FIG. 8, an intermediate layer may enter into the passage when centrifuged, and component blood is separated and collected. Passage 105 when taking out
There is a possibility that even a small intermediate layer may be taken in together.

On the other hand, in the liquid separating apparatus, the lower pressure biasing method in which the lower end of the pressure plate 107 is rotatably supported, if the position of the intermediate layer S3 is to be detected at the time of pressurization,
Since the detection is performed in a large portion of the cross section, a slight error in the detection position greatly affects the blood component separation accuracy, and the separation accuracy may be reduced. In addition, the parallel pressurizing method has a drawback that control when the pressurizing plate 108 is operated in parallel to pressurize the bag is difficult and complicated.

As an improvement on this point, an upper pressure bias system has been proposed (see Japanese Patent Application Laid-Open No. 2-98,362). This upper pressure biasing method has the advantage that the fulcrum of the pressure plate is at the upper side, so that if it is taken out upward, the sectional area of the part to be taken out is small and the error of the detection position of the intermediate layer S3 is small, Since the position of the layer S3 must be tracked and detected, the control structure of the entire apparatus becomes complicated and the cost is high.

The present invention has been made in order to solve the problems associated with the above-mentioned prior art, and a predetermined layer such as the intermediate layer is positioned within a predetermined range in a parent container, and the predetermined layer is slightly Provided is a liquid separating device that can easily take out a fluid in a desired layer without any problem even if the position of the tube fluctuates, and that can take out the liquid only from the upward direction and has good controllability and easy handling of tubes. The purpose is to do.

[0013]

According to the present invention for achieving the above object, at least three layers of an upper layer, an intermediate layer, and a lower layer are provided.
A first layer and a second layer on one side of the upper layer or the lower layer, wherein the first layer has a first outlet and a second outlet.
Container holding portion for holding a flexible parent container having a partition wall extending from a position between the outlet and the second outlet to a predetermined distance from the other end, and a parent held by the container holding portion A liquid separating device having a container pressing portion including a supporting member for supporting one of two opposite surfaces of a container and a pressing member for pressing the other surface, the liquid separating device being formed by the partition wall. An opening and closing clamp means for opening and closing the flow path, the opening and closing clamp means being adjacent to the pressing member, wherein the upper layer comprises a first outlet and a second outlet.
When the intermediate layer is located below the end of the partition wall, and the opening and closing clamp means,
After closing the flow path, the pressing member presses the parent container to draw out the upper layer from the first outlet so that the intermediate layer is located above the end of the partition wall, and then the flow is controlled by the opening / closing clamp means. By opening the path and pressing the parent container again by the pressing member while maintaining the position of the intermediate layer, the lower layer is guided to the second outlet through the flow path and the upper layer is moved to the first outlet.
When the upper layer is on the first and second outlet sides and the intermediate layer is located above the end of the partition wall, the opening and closing clamp means causes And the lower layer is guided to the second outlet and the upper layer is guided to the first outlet through the flow path, and the lower layer is on the first outlet and the second outlet side, and the intermediate layer is partitioned. When it is located above the end of the wall, after closing the flow path by the opening and closing clamp means, the parent member is pressed by the pressing member to guide the lower layer from the first outlet to partition the intermediate layer. After being positioned at the lower part of the wall end, the flow path is opened by the opening / closing clamp means, and the parent container is pressed again by the pressing member while maintaining the position of the intermediate layer. Guide the lower layer to the first outlet, Lower layer is in the first outlet and the second outlet, and when the intermediate layer is positioned below the partition wall end is open a flow path by opening and closing the clamp means,
A liquid separation device characterized in that an upper layer is guided to a second outlet and a lower layer is guided to a first outlet through the flow path. Further, the present invention includes a weight measuring unit that constantly measures the weight of the parent container.

[0014]

According to the present invention, there is used a parent container which extends downward from between the ports and has a partition wall having an end in the middle of the parent container, and opens and closes a passage formed by the partition wall by a clamp means. When the parent container is pressurized by the pressurizing means in a state where the liquid is closed, the liquid in the uppermost layer flows out from a predetermined port. As a result, the predetermined layer such as the intermediate layer also rises, but when the predetermined layer rises beyond the end of the partition wall, the opening and closing clamp means is opened and the parent container is pressurized by the pressurizing means. The liquid in the next layer below the predetermined layer flows out through the passage. The outflow of the liquid in the next layer lowers the position of the predetermined layer, but if the liquid does not lower beyond the end position of the partition wall, the predetermined layer does not flow out through the passage. Therefore, the position management of the predetermined layer is a relatively wide range in which the partition wall is formed, and it is easy to cause a desired liquid to flow out of a desired port, and liquids separated into a plurality of layers are individually taken out. The separation accuracy in the case is improved. In addition, since a plurality of fluid extraction tubes are provided at the upper end of the parent container, components of the fluid can be extracted from one direction, a liquid control device with good controllability, and handling of a plurality of fluid extraction tubes is possible. It's easy. Further, by providing a weight measuring section for constantly measuring the weight of the parent container, the pressing member stops when the remaining weight in the parent container reaches a predetermined weight, and the first
And the second outlet is clamped by a clamp member.

[0015]

An embodiment of the present invention will be described below with reference to the drawings. FIG. 1 is a schematic perspective view of a liquid separation device according to an embodiment of the present invention, FIG. 2 is a schematic cross-sectional view taken along line 2-2 of FIG.
FIG. 3 is a schematic perspective view showing a main part of the present invention, and FIG. 4 is a schematic plan view showing an example of a blood bag.

In FIG. 1, a liquid separating apparatus 10 is for taking out each component blood from a centrifuged blood collection bag B1 (hereinafter simply referred to as a parent bag), and a container holder 11 for holding the parent bag B1. A clamping member 12 for selectively clamping the tube t; and a pressing means 13 for pressing the parent bag B1 held in the container holding portion 11.
Opening and closing clamp means 30 for opening and closing a passage D (see FIG. 4) formed in the parent bag B1;
A control unit C for controlling the pressurizing means 13 and the open / close clamp means 30;

First, the blood bag will be described. FIG.
As shown in the figure, the parent bag B is made of a flexible synthetic resin, and is a blood collection bag for storing blood collected from a donor, a so-called parent bag B1, and the parent bag B1 is connected to the parent bag B1 via tubes t1 and t2. It is composed of a plurality of communicating separation bags, so-called child bags B2a and B2b. Inside the parent bag B1, the plasma layer S1,
The liquid separated into three layers in the order of the intermediate layer S3 and the red blood cell layer S2 is sealed therein, and a plurality of ports P and a through hole 17 for hanging the container holding portion 11 are opened in the upper part.

The empty child bag B2a connected to the parent bag B1 via tubes t1 and t2 has a plasma layer S
1 is taken out, and the red blood cell layer S2 is taken out of the child bag B2b containing red blood cell storage.

In particular, in the parent bag B1 of the present embodiment, a partition wall d extends downward from between the ports P provided on the upper part, and the end of the partition wall d is located in the middle of the parent container B1. Preferably, it is located at a substantially middle position of the parent bag B1. Due to the formation of the partition wall d, a passage D is formed in the parent bag B1.

Next, as shown in FIGS. 1 and 2, the pressurizing means 13 comprises a housing 1 housed in the front part of the main body case 14.
5, a pair of container holders 11 project from a front wall 15a of the housing 15, and a through hole 17 of the parent bag B1 is hung on the container holders 11, so that the parent bag B
1 is suspended.

The housing 15 of the pressurizing means 13 is attached to an aluminum block 45 to be described later. Inside the housing 15, a container pressurized from the abdominal surface and the back side of the parent bag B1 suspended by the container holding portion 11. A pressing unit 18 is provided. The container pressurizing section 18 abuts on the abdominal surface of the parent bag B1 and is stationary when pressurized, a second pressure plate 20 abuts on the back side of the parent bag B1, This second pressure plate 20 is used for the parent bag B1.
And a spring member 21 that presses toward the first pressing plate 19 side.

In the present embodiment, the first pressure plate 19
The housing 15 includes a door that opens and closes a front portion. The door is preferably made of a transparent material such as acrylic resin so that the state of the inner parent bag B1 can be visually checked.

On the other hand, a link mechanism 22 is connected to the second pressure plate 20. This link mechanism 22
A first link 24 rotatably connected to the rear side of the pressure plate 20 by a pin 23; and a support shaft 25 having one end attached to the rear end of the first link 24 and the other end attached to the housing 15. And a second link 26 which is rotatably supported on the second link 26. And the first link 24
One end of the spring member 21 is attached to the end on the side opposite to the pressure plate, and the other end of the spring member 21 is supported near the front wall 15 a of the housing 15. Accordingly, the spring resilience of the spring member 21 initially strongly presses the parent bag B1, but the elastic resilience decreases as the parent bag B1 shrinks. As a result, as the separation from the parent bag B1 to the child bags B2, B2 approaches the end, the spring member 2
1 is reduced, the component blood is carefully flowed from the parent bag B1 to the child bags B2a, B2b, and it is convenient to leave the middle layer S3 in the parent bag B1. It is something with.

In the vicinity of the pressurizing means 13, an opening / closing clamp means 30 shown in detail in FIG. 3 is provided. The opening / closing clamp means 30 has a tapered tip block 31 and a drive means 32 for moving the tip block 31 forward and backward toward the parent bag B1, and is the tip block 31 and the door. The passage D of the parent bag B1 is sandwiched between the first press plate 19 and the first press plate 19 to open and close the passage D. Here, the drive means 32 is preferably a solenoid, but in some cases, various means such as a motor, an air cylinder or a hydraulic cylinder may be used.

The opening / closing clamp means 30 is provided with an intermediate layer S3
To prevent it from flowing out through passage D,
This opening and closing is performed by the pressurizing means 13 pressing the parent container B1.
When the intermediate layer S3 is located above the end of the partition wall d, it is controlled by the control section C so as to be opened.

In addition, FIG. 1 and FIG.
As shown in FIG. 4, the extension of the parent bag B1 from the upper end
The clamp member 12 is provided for independently controlling the flow of the component blood flowing through the tubes t1 and t2. The clamp member 12 includes a first clamp member 40a for opening and closing a tube t1 through which a plasma component flows, and a tube t1 through which a red blood cell component flows.
2 and a solenoid 41 for operating these clamp members 40a and 40b.
a and 41b, and the cut-off control is performed by a signal from the control unit C issued according to the position of the intermediate layer S3 of the centrifuged blood, the weight of the parent bag B1, and the pressurization time. It is supposed to be. And this tube t
The component blood flowing out through 1, t2 is stored in child bags B2a, B2b installed in a tray 42 formed on the upper part of the main body case 14. The tray 42 is closed by an upper lid 43, hermetically sealed by a surrounding packing 44, and the pressure inside the tray 42 is reduced by a pump 45 and a conduit 46. This is to make it easier for the component blood to flow from the parent bag B1 to the child bag B2.

The position of the intermediate layer S 3 is detected by an interface detecting member 50 provided on the second pressure plate 20. The interface detecting member 50 generally uses a photosensor, and detects an interface based on a difference in light absorptance of each of the three layers described above.
The signal from 0 is input to the control unit C.

The weight of the parent bag B1 is measured by a weight measuring section G as shown in FIG. This weight measuring section G
Is attached to an aluminum block 51 to which the pressurizing means 13 is attached. The weight of the parent bag B1 is calculated based on the total weight of the pressurizing means 13 by the housing 15, the first and second pressurizing plates 19, 20, and the like. Is calculated by deducting the tare of. That is, the total weight of the pressurizing means 13 is such that the strain amount of the aluminum block 51 caused by the weight of the pressurizing means 13 is detected by a strain gauge which is a weight sensor 52 attached to the side surface of the aluminum block 51. The pressurizing means 13 is calculated from the total weight.
By subtracting the known weight of, for example, the housing 15, the weight of only the parent bag B1 can be known.

In the drawing, reference numeral "53" denotes a scale stopper.

The pressurizing time is detected by a timer 55 provided on a control panel 54 provided on the front surface of the main body case 14, and a signal from the timer 55 is also input to the control unit C.

Next, the operation of the embodiment will be described. First, the through hole 17 of the parent bag B1 is provided in the housing 15 of the pressurizing means 13.
On the hook 11 and the child bags B2a and B
After installing 2b, the upper lid 47 is closed, and two tubes t
1 and t2 are set between the clamp members 12. Here, the clamp member is in the “open” state and the parent bag B
1 is the state already centrifuged by the centrifuge, and the blood inside is separated into three layers.

When a start switch (not shown) of the control unit C is turned on, the vacuum pump 45 is operated, and the tray 4
2 is also in a reduced pressure state, and both clamp members 40a,
40b is brought into the "closed" state by the solenoids 41a and 41b. As a result, even if the pressure in the tray 42 is reduced, the component blood does not flow out of the parent bag B1. Further, the weight of the parent bag B1 is detected by the weight sensor 52,
The weight value is input to the control unit C. If this weight is the predetermined weight, normal blood is determined. And the first, which is the door
The pressurization is started by closing the pressurizing plate 19, but since the clamp members 40a and 40b are in the "closed" state, the initial position of the intermediate layer S3 of the parent bag B1 is accurately detected by the interface detecting member 50. it can. Here, parent bag B
1 is applied by urging the second pressure plate 20 toward the first pressure plate 19 by the elastic force of the spring member 31. The spring member 21 20 is set so as to exert a resilient force in the direction of pressurizing the parent bag B1, so even if the force of the pressurizing place is strong, the force decreases with the outflow of the component blood inside, and the force is reduced. This will prevent a rapid outflow of the layer S3. The parent bag B1 is pressed between the first pressing plate 19 and the second pressing plate 20 from the abdominal side and the back side. In this pressing state, the door is made of a transparent body. Therefore, the worker is able to see and be convenient.

(A) When the initial position of the intermediate layer S3 detected by the interface detecting member 50 is located below the lower end of the partition wall d, in this case, for example, the two clamp members 40a and 40b are set to " If "open", the middle layer S3 may enter the passage D from the entrance of the passage D when the upper plasma layer S1 flows out of the port P. Therefore, in this case, the opening / closing clamp means 30 is immediately operated to close the entrance of the passage D, and the clamp member 4 is closed.
0a is set to "open", and only the plasma layer S1 is taken out to the child bag B2a.

With the outflow of the plasma layer S1, the plasma layer S1
The position of the intermediate layer S3 below the position gradually rises.
The position of the intermediate layer S3 is above the lower end of the partition wall d,
Further, when the interface detecting member 50 detects that the intermediate layer S3 has reached the upper limit position L1 (see FIG. 4), for example, a position near the port P, the intermediate layer S3 may flow out of the port P. The clamp member 40a of the tube t1 through which the air flows is "closed". However, the opening and closing clamp means 3
0 may remain "open". As a result, although the outflow of the plasma layer S1 is stopped, the lower red blood cell layer S2 flows out of the passage D, and the separation of the component blood can be performed without stopping. Even when the opening / closing clamp means 30 is in the "open" state, the intermediate layer S3 is partitioned by the partition wall d and does not flow out of the passage D.

As a result, the flow of the plasma layer S1 is stopped,
Due to the outflow of the lower red blood cell layer S2, the position of the intermediate layer S3 is lowered. When the interface detecting member 50 detects that the position of the intermediate layer S3 has reached the lower limit position L2 (see FIG. 4) slightly above the lower end of the partition wall d, the opening and closing clamp means 3
0 is set to "closed" to stop the outflow of the red blood cell layer S2 from the passage D. However, the clamp member 40a of the tube t1 for the plasma layer S1 is opened, and the plasma layer S1 flows out again.

(B) When the initial position of the intermediate layer S3 detected by the interface detecting member 50 is located above the lower end of the partition wall d, in this case, the intermediate layer S3
Since there is no possibility that the gas enters the passage D from the entrance of the passage D, the clamp members 40a and 40b are immediately opened and the open / close clamp means 30 is opened, and the upper plasma layer S1 is opened from the port P. The red blood cell layer S2 flows out of the passage D. The subsequent control state is the same as described above.

After taking out each component blood from the parent bag B1 to the child bag B2, the tube t near the child bag B2 is removed.
1, t2 is closed with a clip or the like, the door is opened, and the parent bag B1 and the child bag B2 are removed from the liquid separating device.

As described above, in the present embodiment, the position of the intermediate layer S3, which is not preferably discharged into the small bag, is moved up and down by alternately flowing the plasma layer S1 and the red blood cell layer S2 a plurality of times. Minimize range. As a result, the accuracy of separating component blood is also improved.

The present invention is not limited to the above-described embodiment, but can be appropriately modified and used within the scope of the claims. For example, in the above-described embodiment, a blood bag is used to separate and collect each component blood, but the present invention can be used not only for this but also for separating and collecting various kinds of centrifuged liquids.

In the above-described embodiment, the position of the intermediate layer S3 in the parent bag B1 is detected by the interface detecting member 50 and compared with the initial position. The upper sensor may be provided near the port P, and the lower sensor may be provided slightly above the lower end of the partition wall d.

Further, in the above embodiment, one opening / closing clamp means 30 is provided inside the pressurizing means 13. However, not only this but also an opening / closing clamp means for applying pressure from both sides of the parent bag B1 in some cases. The pressing position by the opening / closing clamp means is not necessarily limited to the lower end of the partition wall d, but may be an intermediate position of the partition wall d in some cases.

Further, as shown in FIG.
The parent bag B1 may be held by the container holding unit 11 with the ports 31, 32 to which 1, 1 and t2 are connected being positioned on the lower side. The pressing means 30 in this case is provided at the upper end of the partition wall d, and in this holding state, the upper layer S1
Is taken out from the lower side of the parent bag B1 through the flow path D.

[0043]

As described above, according to the present invention, a parent container having a partition wall extending downward from between the ports and having a terminal end in the middle of the parent container is used. The parent container is pressurized by the pressurizing means while controlling the opening and closing of the entrance of the passage formed by the opening and closing clamp means, so that the liquid of the desired layer is appropriately adjusted while adjusting the position of the predetermined layer where outflow is undesirable. The liquid can be selectively discharged, and the position management of the predetermined layer can be performed in a relatively wide range, so that the separation accuracy when liquids separated into a plurality of layers are individually taken out is improved. In addition, since a plurality of fluid extraction tubes are provided at the upper end of the parent container, the components of the fluid are taken out only upward, so that the liquid separation device has good controllability and the handling of the plurality of fluid extraction tubes is also possible. It's easy.

[Brief description of the drawings]

FIG. 1 is a schematic perspective view of a liquid separation device according to one embodiment of the present invention,

2 is a schematic sectional view taken along line 2-2 of FIG. 1,

FIG. 3 is a schematic perspective view showing a main part of the present invention,

FIG. 4 is a schematic plan view showing an example of a blood bag.

FIG. 5 is a front view showing another example of the blood bag,

FIG. 6 is a front view showing an example of a conventional blood bag;

FIG. 7 is a schematic front view showing another example of the conventional blood bag.

FIG. 8 is a schematic front view showing still another example of the conventional blood bag,

FIG. 9 is a schematic explanatory view showing an example of a conventional liquid separation device.

FIG. 10 is a schematic explanatory view showing another example of the conventional liquid separation device.

[Explanation of symbols]

12 ... clamping member 13 ... pressurizing means
21: Spring member, 30: Opening / closing clamp means, 50: Interface detecting member, B: Container, B1: Parent container, B2: Small container, C: Control unit, d: Partition wall,
D: passage, G: weight measuring unit,
P ... Port, S1, S2, S3
... a liquid layer, t ... a fluid extraction tube.

──────────────────────────────────────────────────続 き Continued on front page (58) Field surveyed (Int. Cl. ⁷ , DB name) A61M 1/00-1/36

Claims (1)

(57) [Claims] 1. A component separated into at least three layers of an upper layer, an intermediate layer, and a lower layer is housed therein, and a first outlet and a second outlet are provided on one side of the upper layer or the lower layer. A container holder for holding a flexible parent container having a partition wall extending from a position between the first outlet and the second outlet to a predetermined distance before the other end; A liquid separating device comprising: a container pressing portion including a supporting member for supporting one of two opposing surfaces of a held parent container and a pressing member for pressing the other surface; Opening / closing means for opening / closing the flow path formed by the pressure member, the upper layer being on the first outlet and the second outlet side, and the intermediate layer being closer to the end of the partition wall. If it is located below,
After closing the flow path by the opening / closing clamp means, the pressing member presses the parent container to draw out the upper layer from the first outlet so that the intermediate layer is positioned above the end of the partition wall. By opening the flow path by the opening / closing clamp means and pressing the parent container again by the pressing member while holding the position of the intermediate layer, the lower layer is guided to the second outlet through the flow path and the upper layer is moved to the first outlet. When the upper layer is on the first and second outlet sides and the intermediate layer is located above the end of the partition wall, the opening / closing clamp means provides a flow path. And guiding the lower layer to the second outlet and the upper layer to the first outlet through the flow path, further comprising the lower layer on the first outlet and the second outlet side,
And when the intermediate layer is located above the end of the partition wall, after closing the flow path by the opening and closing clamp means, the pressing member presses the parent container to draw out the lower layer from the first outlet. After the intermediate layer is positioned at the lower part of the end of the partition wall, the flow path is opened by the opening and closing clamp means, and the parent container is pressed again by the pressing member while maintaining the position of the intermediate layer. Guiding the layer to the second outlet and guiding the lower layer to the first outlet, wherein the lower layer is on the first outlet and the second outlet side, and the intermediate layer is below the end of the partition wall. A liquid separation device, wherein the flow path is opened by the opening and closing clamp means, and the upper layer is guided to the second outlet and the lower layer is guided to the first outlet through the flow path.