JP3167476B2 - Liquid separation device - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a liquid separating apparatus for taking out a liquid separated into a plurality of layers, such as a blood bag, from a sealed flexible container for each layer of liquid.

[0002]

2. Description of the Related Art In recent years, instead of whole blood transfusion, component transfusion for transfusing only necessary components in blood to a patient or the like has been performed, or only plasma has been collected from blood for preparing a plasma preparation. Has also been done. In order to obtain such blood for component transfusion and plasma preparation, a flexible blood bag called a so-called multi-bag has been conventionally used. This blood bag is composed of a blood collection bag (so-called parent bag) for storing blood collected from a donor, and one or more separation bags (so-called child bags) connected to the parent bag via a tube. The blood components in the configured and centrifuged parent bag are taken out for each component and taken into the child bag.

When a bag for blood collection is centrifuged, the blood becomes a plasma layer, an erythrocyte layer and an intermediate layer (a buffy coat layer which is a buffy coat layer containing platelets, leukocytes or fats, and further various bacteria, etc.). Is separated for each component, but in order to use the separated components later, it is necessary to separate and collect the components from the blood collection bag into each separation bag so that the components do not mix with each other. .

[0004] In order to perform such sorting and collection, a liquid separating device is required in addition to the blood bag. Examples of such a blood bag include those shown in FIGS. FIG.
In the blood bag 100A shown in FIG. 1, a tube is attached to an upper portion of a parent bag 101 that stores collected blood. Tubes t1 to t3 are connected to the tubes via two T-shaped branch pipes, and a distal bag 102a containing a red blood cell preservation solution is provided at the tip of each of the tubes t1 to t3.
And two empty child bags 102b and 102c are connected.

In order to separate the components from the parent bag 101, first, the centrifugal separation is performed on the abdominal surface of the parent bag 101 containing the blood separated into the three layers of the plasma layer S1, red blood cell layer S2, and intermediate layer S3. To transfer the plasma component forming the upper layer S1 to the child bag 102b via the tube t2. When the transfer of the plasma components is completed, a small amount of the plasma components remaining in the parent bag 101, the components of the intermediate layer, that is, the buffy coat layer, and a part of the red blood cell components of the lower layer are transferred to the child bag 102c via the tube t3. Transfer. When the intermediate layer is removed from the parent bag 101 in this way, the erythrocyte preservation liquid contained in the child bag 102a is then transferred to the parent bag 101 via the tube t1.
Transfer to The blood bag 100A that separates blood components in this way is practical because the components can be collected from one direction of the parent bag 101 (in this example, from above).

A blood bag 100B shown in FIG. 11 has tubes t1 and t2 provided above and below a parent bag 101. The parent bag 101 is separated into the three layers of the plasma layer S1, red blood cell layer S2, and intermediate layer S3 by centrifugation. So that the plasma layer S1 is taken out from the upper tube t1 into the child bag 102a, the red blood cell layer S2 is taken out from the lower tube t2 into the child bag 102b, and the intermediate layer S3 is left in the parent bag 101. (Tokuno Sho 6)
3-20, 144). This blood bag 100
In B, the desired components are respectively taken up and down from the centrifuged state, so that the intermediate layer can be efficiently separated and collected from the blood. Note that reference numeral “103” in FIGS. 10 and 11 denotes a blood collection needle.

In the blood bag 100C shown in FIG. 12, a seal portion 104 extending to the bottom of the bag is formed by heat-sealing the front and back surfaces of the parent bag 101, and thereby a passage 105 for taking out upward is formed in the bag. It is formed independently. Then, the centrifuged parent bag 10
1 is attached to a liquid separation device, and the first
The plasma layer S1 is transferred from the tube t1 of the second tube t1.
2, the red blood cell layer S2 is taken out and the intermediate layer S3 is left in the parent bag 101 (see European Patent Publication No. 0484751A1). This blood bag 100C is practical because each component can be taken out from above.

On the other hand, in the liquid separating apparatus, the centrifuged parent bag 101 is pressurized to flow the plasma layer S1 toward the first child bag 102a and the red blood cell layer S2 toward the second child bag 102b. A vessel pressurizing unit is provided. Conventionally, the container pressurizing unit employs a method of pressurizing the parent bag by using a lower pressure biasing method as shown in FIG. 13 (for example, see Japanese Patent Publication No. 55-17,585).
And a parallel pressure type as shown in FIG. 14 (for example, see Japanese Patent Application Laid-Open No. 55-155,652). In the former, the parent bag 101 is hung on a hook 36 provided on a separation stand 106, and the lower surface of the bag 101 is pressed by a pressing plate 107 whose lower end is rotatably supported. A pressure plate 108 is provided in parallel with the stand 106, and the abdominal surface of the bag 101 is pressed in this parallel state. These include automatic pressurization and manual pressurization. In the automatic pressurization which is frequently used recently, any of these methods detects the position of the intermediate layer S3 with an interface sensor (not shown). The parent bag 101 is pressed by the pressing plate 107 or 108.

As shown in FIGS. 11 and 12, the liquid separating apparatus has clamp members 40a and 40 for independently blocking the flow of the fluid flowing through the tubes t1 and t2.
b are provided, and these clamp members 40a, 40
b indicates that the intermediate layer S3 detected by the interface sensor is a tube t.
It is individually driven to open and close so as not to be taken in at 1 or t2.

For example, when the intermediate layer S3 is displaced upward by more than an allowable size from the initial position when the intermediate layer S3 is centrifuged by continuing to pressurize the parent bag 101, the clamp member 40a for the tube t1 is driven to close. Thus, the tube t1 is closed, and the removal of the plasma layer S1 is temporarily stopped. Then
When only the red blood cell layer S2 is taken out by pressurizing the parent bag 101 and the intermediate layer S3 is lowered to a position within an allowable range, the clamp member 40a is driven to open, and the taking out of the plasma layer S1 is resumed.

[0011]

However, in the case of taking out the intermediate layer S3 from the parent bag 101, such as the blood bag 100A shown in FIG. 11, the components of the intermediate layer S3 are deposited on the inner wall of the parent bag 100A. The intermediate layer S
It was difficult to completely remove 3 from the parent bag 101.

Further, in the conventional liquid separation apparatus, while tracking the constantly changing position of the intermediate layer S3, the opening / closing drive control of the clamp members 40a and 40b and the parent bag 1 are controlled.
Since the pressurization control of 01 must be performed, there is a disadvantage that the control configuration of the entire apparatus becomes complicated.

SUMMARY OF THE INVENTION The present invention has been made to solve the problems associated with the above-mentioned prior art, and does not require complicated control from a parent container in which a liquid separated into at least three layers is enclosed. It is an object of the present invention to provide a liquid separation device that can be separated into fluids for each layer and has high separation accuracy.

[0014]

According to the present invention, which achieves the above object, the present invention provides a liquid crystal display device in which components separated into at least three layers of an upper layer, an intermediate layer, and a lower layer are accommodated, and the upper layer or the lower layer is contained therein. A container holding portion for holding a flexible parent container provided with a first outlet and a second outlet on one side of the container, and two opposing surfaces of the parent container held by the container holding portion Of 1
A liquid separating device having a container pressing portion including a support member for supporting one surface and a pressing member for pressing the other surface, wherein a first member for partitioning the upper layer and the intermediate layer is provided.
Partitioning means, second partitioning means for partitioning the intermediate layer and the lower layer, and interface detection provided between the first partitioning means and the second partitioning means for detecting an interface of the intermediate layer Means, the pressing member comprises a first pressing member, a second pressing member, and a third pressing member adjacent to the first and second pressing members.
When the first outlet and the second outlet are on the side of the upper layer, the first outlet and the second outlet are pressed by the first pressing member to press the upper layer partitioned by the first partitioning means. Guiding the upper layer to the first outlet, pressing the lower layer partitioned by the second partitioning means with the second pressing member, and pressing the first outlet and the first outlet with the third pressing member. A contact portion is formed on two opposing surfaces of the parent container from between the second outlet and at least across the intermediate layer to the lower layer, and the second outlet is formed in the parent container by the contact portion. Forming a passage communicating with the lower layer and pressing the lower layer, guiding the lower layer to the second outlet through the passage, wherein the first outlet and the second outlet are on the side of the lower layer. In some cases, the second pressing member allows the second partitioning means to Pressing the divided lower layer, guiding the lower layer to the second outlet, pressing the upper layer partitioned by the first partitioning means with the first pressing member, 3 the pressing member opposes the main container from between the first outlet and the second outlet at least across the intermediate layer to the upper layer.
A contact portion is formed on the surface, and a passage communicating with the first outlet is formed in the parent container by the contact portion to press the upper layer, and the upper layer passes through the passage to form the upper layer. 1 is a liquid separation device, which is led to one outlet.

Further, the present invention is characterized in that a weight measuring unit for constantly measuring the weight of the parent container is provided.

[0016]

According to the first aspect of the present invention, the first and the second are provided.
Because the intermediate layer is separated from other layers by the partitioning means,
Even when the parent container is pressurized, that is, when the fluid of the other layer is flowing out, the position of the intermediate layer does not change. Therefore,
Since there is no danger of the intermediate layer flowing out of the parent container, separation of the fluid without flowing out of the intermediate layer from the parent container is performed.
Control during this separation operation is also easy.

When the first outlet and the second outlet are on the upper layer side by the third pressing member, a passage for guiding the fluid of the lower layer to the second outlet is formed in the parent container. When the first outlet and the second outlet are on the side of the lower layer, a passage for guiding the fluid of the upper layer to the first outlet can be defined in the parent container. For this reason, by connecting a plurality of fluid extraction tubes to one end of the parent container, components of the fluid can be extracted from one direction, and handling of the fluid extraction tubes is simplified.

Further, even when the interface position of the intermediate layer is different for each parent container, the intermediate layer can be partitioned from other layers by the first and second partition means, and the intermediate layer flows out of the parent container. Separation of the fluid without causing it to occur.

[0019]

An embodiment of the present invention will be described below with reference to the drawings. FIG. 1 is a schematic perspective view of a liquid separation device according to one embodiment of the present invention, FIG. 2 is a schematic plan view showing an example of a blood bag, and FIG. 3 is a schematic sectional view taken along line 3-3 in FIG. , FIG.
Is a horizontal sectional view showing a main part of FIG. 1, FIG. 5 is a side view showing a partial cross section of the container pressurizing unit, and FIG. 6 is a perspective view showing a main part of the container pressurizing unit shown in FIG. , FIG. 7 (1)
(4) is explanatory drawing which shows the pressurizing operation | movement of a container pressurizing unit.

In FIG. 1, a liquid separating apparatus 10 is for taking out each component blood from a centrifugally separated blood bag B, and selectively includes a container holding section 11 for holding the blood bag B and tubes t1 and t2. Clamping member 1 for clamping
2, a container pressurizing unit 13 for pressurizing the parent bag B1 held by the container holding portion 11, and a container pressurizing unit 1
And a control unit C for controlling 3 and the like.

The blood bag B is made of a flexible synthetic resin, and as shown in FIG. 2, a blood collection bag for storing blood collected from a donor, a so-called parent bag B1 (corresponding to a parent container). The parent bag B1 is composed of a separation bag, so-called child bag B2a and child bag B2b, which are communicated with each other through first and second tubes t1 and t2. A blood bag B in which a plurality of child bags B2a ..., B2b ... are connected to the first and second tubes t1, t2, respectively.
There is also.

At the top of the container of the parent bag B1, a first port Q1 (corresponding to a first outlet) to which a first fluid outlet tube t1 is connected, and a second port to which a second fluid outlet tube t2 is connected. Q2 (corresponding to the second exit). Further, a partition wall d is formed in the parent bag B1 so as to partition the first port Q1 and the second port Q2 by heat-sealing the front and back surfaces of the bag. The partition wall d of the present embodiment does not extend to the bottom of the bag like the seal portion 104 shown in FIG. An internal passage D is formed by the partition wall d and a pressing piece 75 provided on the container pressurizing means 18 described later. When the blood in the parent bag B1 is centrifuged, the plasma layer S
1 (corresponding to the upper layer), the erythrocyte layer S2 (corresponding to the lower layer) and the middle layer S3, which are used for the plasma layer S1 and the erythrocyte layer S2. The component blood of the plasma layer S1 is taken out of the parent bag B1 through the first port Q1 and further taken into the first child bag B2a via the first tube t1. The component blood of the red blood cell layer S2 is guided through the internal passage D to the second port Q2, is taken out from the second port Q2, and is further removed from the second tube t2.
2 and is taken into the second child bag B2b into which the erythrocyte preservation solution has been previously injected. When the erythrocyte layer S2 flows out through the internal passage D, the intermediate layer S3 may be mixed to some extent, but experiments have shown that there is no problem.

As shown in FIGS. 1 and 3, the container pressurizing unit 13 is provided in a housing 15 provided in a front part of a main body case 14, and a front wall 1 of the housing 15 is provided.
A pair of hooks 11 protrude from 5a. A through hole 17 formed in the parent bag B1 is hung on the hook 11 to serve as a container holder for suspending the parent bag B1.

Housing 15 of container pressurizing unit 13
Is attached to the aluminum block 45 and the housing 1
As shown in detail in FIG.
1 from the back side of the parent bag B1
1 is provided with a pressure block 60 which can be pressed against the container 1 and a container pressurizing means 18 which pressurizes the parent bag B1.

As shown in FIG. 6, the pressure block 60 has a substantially convex shape, and the convex tip is disposed so as to face the back of the parent bag B1.
The main bag B1 is provided so as to be able to move back and forth along the horizontal direction from the back side to the abdomen side and to be able to move up and down along the vertical direction of the parent bag B1. A first convex portion 61 (corresponding to a first partitioning means) that presses the parent bag B1 between the pressure block 60 and a support plate 19 described below to partition the plasma layer S1 and the intermediate layer S3. And the support plate 19
A second convex portion 62 (corresponding to a second partitioning means) for partitioning the red blood cell layer S2 and the intermediate layer S3 by pressing the parent bag B1 therebetween is formed vertically above and below a predetermined gap. . The weight of the intermediate layer S3 is usually 40 to 50 g or less. However, in order to eliminate the possibility that the fluid of the intermediate layer S3 is taken into the child bags B2a and B2b, the gap between the two convex portions 61 and 62 is set to 80.
The size is set to contain about 150 g.

A groove 63 having a predetermined depth is provided between the two convex portions 61 and 62 so that a part of the parent bag B1 escapes when the parent bag B1 is pressed. Also,
An interface detecting member 43 (corresponding to an interface detecting means) for detecting the interface of the intermediate layer S3 is provided between the groove 63, that is, between the two convex portions 61 and 62. As the interface detecting member 43, a photosensor composed of a light emitting element and a light receiving element is generally used. The interface detecting member 43 detects the interface based on the difference in the light absorptivity of each of the three layers described above. The signal from the member 43 is input to the control unit C. The groove 63 may be provided with a suction member for preventing the intermediate layer S3 from spreading in the vertical direction.

A guide pin 65 extending horizontally toward the support plate 19 is attached to a holding plate 64 provided vertically movable within the housing 15. A guide hole 67 that extends horizontally and is slidably fitted to the guide pin 65 is formed in the leg portion 66 located in the vertical direction of the pressure block 60. The rack 69 attached to the pressure block 60 is engaged with a pinion gear 68 attached to the holding plate 64, and a motor (not shown) is connected to the pinion gear 68. By rotating the pinion gear 68 clockwise in FIG. 5, the pressure block 60 moves forward toward the support plate 19 while being guided by the guide pins 65. Conversely, the pinion gear 68
Is rotated in the counterclockwise direction in FIG. 5, the pressure block 60 moves backward in a direction away from the support plate 19. When the pressure block 60 moves to the forward limit position, the first convex portion 61 and the second convex portion 62
A support plate 19 is provided between the back and the abdomen of the parent bag B1.
Press against

The container pressurizing means 18 (corresponding to a container pressing portion) abuts against the abdominal surface of the parent bag B1 and is stationary when pressurized. A first pressing plate 20a (corresponding to a first pressing member) and a second pressing plate 20b (corresponding to a second pressing member) contacting the back side of B1; Plate 20a, 20b is parent bag B1
, Ie, drive members 21a and 21b for applying pressure toward the support plate 19 side.

In this embodiment, the support plate 19 is constituted by a door which opens and closes a front portion of the housing 15. The door is preferably made of a transparent material such as acrylic resin so that the state of the inner parent bag B1 can be visually checked. By using the door as the support plate 19 without providing an independent support member, the number of components can be reduced and the configuration can be simplified.

First and second pressure plates 20a, 20b
As shown in FIG. 6, the base is rotatably supported by support shafts 23a and 23b attached to the first and second protrusions 61 and 62.

The driving members 21a and 21b are connected to a housing 70 attached to the leg 66 of the pressure block 60.
A screw portion 71 rotatably held by the housing 70; a nut portion 72 movably attached to the screw portion 71; and a motor 7 for driving the screw portion 71 to rotate.
And 3. The screw portion 71 and the nut portion 72 are configured by ball screws, and the nut portion 72 is attached to the housing 70 so as to move in the horizontal direction in the drawing by rotation of the screw portion 71. The nut 72 and the first and second pressure plates 20a, 20b are connected via connecting rods 74 whose both ends are rotatably supported. The nut portion 7 is rotated by driving the screw portion 71 in an appropriate direction by a motor 73.
2 moves in the horizontal direction, and with the movement of the nut portion 72, the first and second pressure plates 20a and 20b move in the direction toward the support plate 19 around the support shafts 23a and 23b, or It turns in the direction away from 19.

The container pressurizing means 18 further includes a parent bag B1.
And an internal passage forming means for partitioning and forming an internal passage D (corresponding to a passage) for guiding the fluid of the lower layer, that is, the red blood cell layer S2 to the second port Q2, in the parent bag B1. In this embodiment, the internal passage forming means is, as shown in FIGS.
Pressing piece 7 attached to one side surface of extension 0 and extending vertically
5 (corresponding to a third pressing member).
Since the pressing piece 75 is provided at the distal end of the pressure block 60, the pressing piece 75 comes into contact with the parent bag B1 as the first convex portion 61 and the second convex portion 62 of the pressure block 60 press against the parent bag B1. Press against. Under the state where the pressing piece 75 is pressed against the parent bag B1, as shown by the phantom line in FIG. 2, the pressing piece 75 extends from the lower end of the partition wall d formed on the parent bag B1 to the vicinity of the bottom of the bag B1. As a result, the inner passage D for guiding the fluid of the red blood cell layer S2 to the second port Q2 is formed in the parent bag B1.

As shown in FIG. 2, the pressing piece 75 extends in the vertical direction at a position closer to the center of the bag by a fixed distance from the side of the second port Q2 of the parent bag B1. The first convex portion 61 and the second convex portion 62 extend in the horizontal direction from the pressing piece 75 toward the other end of the bag. still,
At the lower end of the pressing piece 75, the pressing piece 75 is
When moving up and down in conjunction with 1, 62, it is only necessary to extend to at least the lower second convex portion 62. The pressing piece 75 does not necessarily have to be moved up and down. In the case of the parent bag B1 having the partition wall d as shown in FIG. 2, the pressing piece 75 does not have to reach the upper end of the parent bag B1.
Also, as shown in FIG.
May be formed. In this case, the pressing piece 7
5 reaches the upper end of the parent bag B1. The present invention can be applied to such a parent bag B1. In this case, not only can the production of the parent bag B1 be facilitated with the elimination of the partition wall d, but also the liquid separation device 10 can be used. At the time of attachment, the parent bag B1 can be attached without being aware of the front and back of the parent bag B1, making the attachment work extremely easy.

As shown in FIG. 5, the container pressurizing unit 13 further detects the pressure block 60 and the container pressurizing means 18 and the pressing piece 75 attached to the pressure block 60 by the interface detecting member 43. There is provided a moving unit 76 for moving along the vertical direction according to the interface position. More specifically, in the housing 15 of the container pressurizing unit 13,
A base plate 77 that supports the holding plate 64 so as to freely move up and down is attached. The base plate 77 is bent at both upper and lower ends to form a screw holding portion 78. The upper and lower ends of the holding plate 64 are also bent to form a nut holding portion 79. A screw portion 80 is rotatably held between the screw holding portions 78 of the base plate 77, and a nut portion 81 attached to the screw portion 80 is attached to a nut holding portion 79 of the holding plate 64. A motor 82 for rotating and driving the screw portion 80 is attached to the upper screw holding portion 78,
Further, the screw portion 80 and the nut portion 81 are constituted by ball screws. The rotation of the screw portion 80 causes the holding plate 64
A long hole 84 is formed in the base plate 77 along the up and down direction, in which the guide portion 83 connected to the nut holding portion 79 of the holding plate 64 slides in order to move up and down.

The controller C first drives the motor 82 to move the holding plate 64 up and down while moving the holding plate 64 up and down.
From the intermediate layer S3 based on this signal.
Is detected. Next, the control unit C controls the intermediate layer S
In order to position 3 between the first convex portion 61 and the second convex portion 62, the holding plate 64 is moved upward,
It is determined whether to move downward. Then, the control section C outputs a control signal to the motor 82 to rotate the motor 82 in the appropriate direction in the forward and reverse directions based on the determination, so that the intermediate layer S3 is located almost halfway between the two convex portions 61 and 62. Upon detecting the position, a stop signal is output to the motor 82.

In the upper part of the main body case 14, FIG.
As shown in FIGS. 3 and 4, a tray 42 for storing child bags B2a and B2b is formed. The tray 42 is closed by an upper lid 47, hermetically sealed by a surrounding packing 48, and the inside thereof is reduced in pressure by a pump P and a guide tube 49. This is to facilitate the flow of the component blood from the parent bag B1 into the child bags B2a and B2b.

Further, as shown in FIGS. 1 and 4, the flow of the component blood flowing through the two tubes t1 and t2 extending from one end of the parent bag B1 is provided at the upper part of the main body case 14. The clamp members 12 are provided to open and shut off each of them independently. The clamp member 12 comprises a first clamp member 40a for opening and closing a tube t1 through which the plasma component S1 flows, and a second clamp member 40b for opening and blocking a tube t2 through which the red blood cell component S2 flows. And these clamp members 4
It has solenoids 41a and 41b for operating 0a and 40b. A signal is sent from a control unit (not shown) to the solenoids 41a and 41b, and based on this signal, the opening and closing control of each of the clamp members 40a and 40b is individually performed. I have. For each component blood S1 and S2, from parent bag B1 to child bag B
2a and B2b, the clamping members 4
Tubes t1 and t2 are shut off by 0a and 40b, respectively. In the liquid separation device 10 of the present embodiment, the intermediate layer S3 is formed by the two convex portions 61 and 62 of the pressure block 60.
Is preliminarily partitioned from the upper layer S1 and the lower layer S2, the opening / closing control of each of the clamp members 40a, 40b is not performed so as not to change the position of the intermediate layer S3.

The symbol "G" in the figure is a weight measuring unit provided for continuously measuring the weight of B1 of the parent bag.
“44” is a weight sensor including a strain gauge attached to an aluminum block 45 provided to support the housing 15 of the container pressurizing unit 13, and “46” is a scale stopper. The weight of the parent bag B1 held in the container holding unit 11 is measured by the weight measuring unit G to determine whether the parent bag B1 is conformable or nonconforming, and by continuously measuring the weight of the parent bag B1. Is always detected. Then, during the liquid separation, when the remaining weight in the parent bag B1 measured by the weight measuring section G reaches a predetermined weight, the operation of the container pressurizing means 18 is stopped, and the tube t1 is clamped by both clamp members 40a and 40b. , T2 are shut off, and the withdrawal of liquid from the first and second ports Q1, Q2 is stopped.

Next, the operating state of the present embodiment will be outlined.

First, a pressing piece 75 as a third pressing member intervenes between the two opposing surfaces of the parent container, between the first and second ports Q1 and Q2 located on the upper side, across the intermediate layer S3 and the lower layer. A contact portion reaching S2 is formed, and the contact portion forms an internal passage D communicating the lower layer S2 and the second port Q2 in the parent bag B1. And the first
The component of the upper layer S2 partitioned from the intermediate layer S3 by the first convex portion 61 as the partitioning means is taken out from the first port Q1 by being pressed by the first pressing plate 20a as the first pressing member. . On the other hand, the component of the lower layer S2 partitioned from the intermediate layer S3 by the second convex portion 62 as the second partitioning means is pressed by the second pressing plate 20b as the second pressing member, thereby forming the internal passage. It is guided to the upper second port Q2 through D and taken out from the second port Q2. That is, the components of the lower layer S2 are also extracted from above.

The above is the schematic operation of this embodiment.
This will be described in detail with reference to (1) to (4).

First, as shown in FIG. 7A, the blood bag B is set in the housing 15 of the container pressurizing unit 13. Here, the parent bag B1 has been already centrifuged by a centrifuge, and the blood inside has been separated into three layers S1, S2 and S3. The bag B is set by hooking the through hole 17 of the parent bag B1 with the hook 11, placing the child bags B2a, B2b connected to the tubes t1, t2 extending from the parent bag B1 in the tray 42, and then placing the upper lid on the tray 42. This is done by closing 47. Further, the support plate 19 as the door is closed.

Next, as shown in FIG. 7 (2), while the motor 82 of the moving means 76 is driven to move the holding plate 64 up and down, the interface position of the intermediate layer S3 is determined based on the signal from the interface detecting member 43. To detect. Then, the motor 82 is driven to rotate in the forward or reverse direction in accordance with the interface position of the intermediate layer S3, and the intermediate layer S3 is positioned almost halfway between the first convex portion 61 and the second convex portion 62 of the pressure block 60. Then, the motor is stopped.

Next, the pinion gear 68 is driven in the clockwise direction in FIG. 5 to advance the pressure block 60 toward the support plate 19 while being guided by the guide pins 65. When the pressure block 60 moves to the forward limit position, as shown in FIG. 7C, the first convex portion 61 and the second convex portion 62 are pressed against the support plate 19 via the back surface and the abdominal surface of the parent bag B1. , The intermediate layer S3 is sealed between the two convex portions 61 and 62. That is, the plasma layer S1 and the intermediate layer S3 are partitioned by the first convex portion 61, and the red blood cell layer S2 and the intermediate layer S3 are partitioned by the second convex portion 62. Further, as the pressure block 60 comes into pressure contact with the parent bag B1, the pressing piece 75 as the pressure contact internal passage forming means comes into pressure contact with the parent bag B1, and the pressing piece 75 and the partition wall d formed on the parent bag B1. An internal passage D for guiding the fluid of the red blood cell layer S2 to the second port Q2 is formed in the parent bag B1.

When the sealing of the intermediate layer S3 is completed, FIG.
As shown in (4), the driving member 21 of the container pressing means 18
a, 21b are driven to move the first and second pressure plates 20a, 20b respectively to the support shaft 23.
It rotates in the direction toward the support plate 19 side around a and 23b. First and second pressure plates 20a, 20b
With this rotation of the main bag B1, the support bag 19 and the first and second pressing plates 20a,
0b, and the plasma layer S1 in the parent bag B1 is sent to the child bag B2a through the first port Q1 and the first tube t1. The red blood cell layer S2 is
It is sent to the child bag B2b through the internal passage D, the second port Q2, and the second tube t2.

After a lapse of a predetermined time, the driving member 2
The motors 1a and 21b are simultaneously or individually reversely driven to rotate the first and second pressure plates 20a and 20b in directions away from the support plate 19 about the support shafts 23a and 23b. . With this rotation, the pressurization of the parent bag B1 is released, and the outflow of the component blood of the plasma layer S1 and the red blood cell layer S2 is stopped. Also, the middle layer S
3 That is, the buffy coat layer remains in the parent bag B1.

When the respective component blood is taken out of the parent bag B1 into the child bags B2a and B2b in this way, the tubes t1 and t2 are closed by clips and the like, and the pinion gear 68 is closed.
Is driven counterclockwise in FIG. 5 to move the pressure block 60 backward in a direction away from the support plate 19. Further, the door 19 is opened, and the parent bag B1 and the child back B are opened.
When 2a and B2b are removed from the liquid separation device 10, a series of operations for separating blood into component blood is completed.

The liquid separating apparatus 10 of this embodiment has convex portions 61 and 62 as partitioning means before the parent bag B1 is pressurized.
Thus, the intermediate layer S3 is separated from the other layers S1 and S2, so that the position of the intermediate layer S3 does not fluctuate even when the parent bag B1 is pressurized, that is, even when component blood flows out. For this reason,
Compared with the case where the opening / closing drive control of the clamp member and the pressurization control of the parent bag B1 are performed while tracking and detecting the constantly changing position of the intermediate layer S3, the control becomes extremely easy. Further, since the intermediate layer S3 is partitioned from the other layers S1 and S2, the intermediate layer S3 does not flow out of the parent bag B1 and blood separation with high separation accuracy can be performed.

Further, in this embodiment, the pressing piece 75 is provided as internal passage forming means for forming the internal passage D for guiding the fluid of the lower red blood cell layer S2 to the second port Q2 in the parent bag B1. The parent bag B1 can be provided with two ports Q1 and Q2 at the top, and when the parent bag B1 is attached to the liquid separating device 10, the tubes t1 and
Handling of t2 is simplified.

Further, a moving means 76 for moving the pressure block 60, the container pressurizing means 18 and the like in the vertical direction according to the interface position of the intermediate layer S3 is provided.
Even if the interface position 3 is different for each parent bag B1, blood can be accurately separated.

It should be noted that the present invention is not limited to only the above-described embodiment, but can be appropriately modified and used within the scope of the claims. For example, in the above-described embodiment, the blood is separately collected for each component blood using a blood bag.
The present invention can be used for separating and collecting various liquids in addition to the above. In addition, the case where the liquid separated into three layers is separated has been described. However, the present invention is applied to a parent container in which the liquid separated into four or more layers is enclosed by increasing the partitioning means. It is possible.

In the above-described embodiment, the case where both the first and second convex portions 61 and 62 as the first and second partitioning means are provided in one pressure block 60 has been described.
The partitioning means can be independently pressed against the parent bag B1 independently,
It may be provided movably in the vertical direction. Container pressurizing means 18
Also, the present invention is not limited to the illustrated example, and may include, for example, a pressing plate using a parallel pressing method.

Further, although the case where the first and second ports Q1 and Q2 are located on the upper layer S1 side is shown in the figure, the present invention, as shown in FIG. It is also applicable to the case where the parent bag B1 is held by the container holding unit 11 with the first and second ports Q1 and Q2 to which the tubes t1 and t2 are connected being positioned on the lower side when the tube is on the S2 side. can do. In this case, the pressing piece 75 as the third pressing member intersects at least the intermediate layer S3 from between the first and second ports Q1 and Q2 located on the lower side between the two opposing surfaces of the parent container. A contact portion reaching the upper layer S1 is formed, and an internal passage D communicating the upper layer S1 and the first port Q1 is formed in the parent bag B1 by the contact portion. The component of the lower layer S2 partitioned from the intermediate layer S3 by the second convex portion 62 as the second partitioning means is pressed by the second pressing plate 20b as the second pressing member, thereby forming the second port. Q2
Taken out. On the other hand, the component of the upper layer S1 partitioned from the intermediate layer S3 by the first convex portion 61 as the first partitioning means is pressed by the first pressing plate 20a as the first pressing member, thereby forming the internal passage. It is guided to the lower first port Q1 through D and taken out from the first port Q1. That is, the components of the upper layer S1 are also extracted from below.

[0054]

As described above, according to the present invention, since the intermediate layer is separated from the other layers by the first and second partition means, the intermediate layer is separated from the other layers during the pressurization of the parent container. Even when the fluid is flowing out, the position of the intermediate layer does not fluctuate. Therefore, since there is no possibility that the intermediate layer flows out of the parent container, the separation of the fluid with high separation accuracy without causing the intermediate layer to flow out of the parent container can be performed without complicated control.

Further, since the third pressing member is provided, when the first outlet and the second outlet are on the upper layer side, a passage for guiding the fluid of the lower layer to the second outlet is defined in the parent container. If the first outlet and the second outlet are on the side of the lower layer, a passage leading the fluid of the upper layer to the first outlet can be defined in the parent container, By connecting a plurality of fluid extraction tubes to one end of the parent container, components of the fluid can be extracted from one direction, and handling of the fluid extraction tubes is simplified.

Further, even when the interface position of the intermediate layer is different for each parent container, the intermediate layer can be separated from other layers by the first and second partitioning means, and the intermediate layer flows out of the parent container. It is possible to separate the fluid without causing the separation.

[Brief description of the drawings]

FIG. 1 is a schematic perspective view of a liquid separation device according to one embodiment of the present invention,

FIG. 2 is a schematic plan view showing an example of a blood bag;

FIG. 3 is a schematic sectional view taken along line 3-3 in FIG. 1;

FIG. 4 is a horizontal sectional view showing a main part of FIG. 1,

FIG. 5 is a schematic side view showing the container pressurizing unit in a partial cross section.

FIG. 6 is a perspective view showing a main part of the container pressurizing unit shown in FIG. 5;

FIGS. 7 (1) to 7 (4) are explanatory views showing a pressurizing operation of a container pressurizing unit.

FIG. 8 is a schematic plan view showing a blood bag according to another embodiment,

FIG. 9 is a schematic plan view showing a blood bag according to still another embodiment.

FIG. 10 is a front view showing an example of a conventional blood bag;

FIG. 11 is a schematic front view showing another example of a conventional blood bag.

FIG. 12 is a schematic front view showing still another example of the conventional blood bag,

FIG. 13 is a schematic explanatory view showing an example of a conventional liquid separation device.

FIG. 14 is a schematic explanatory view showing another example of a conventional liquid separation device.

[Explanation of symbols]

DESCRIPTION OF SYMBOLS 11 ... Hook (container holding part) 13 ... Container pressurizing unit 18 ... Container pressurizing means (container pressing part) 19 ... Support plate (support member) 20a ... First pressurizing plate (first pressing member) 20b ... Second Pressing plate (second pressing member) 43 ... Interface detecting member (interface detecting means) 61 ... First convex part (first dividing means) 62 ... Second convex part (second dividing means) 75 ... Pressing piece (third) B. Container B1 Parent bag (parent container) B2a First child container B2b Second child container D Internal passage Q1 First port (first outlet) Q2 Second port (second container) Outlet) S1 ... Plasma layer (upper layer) S2 ... Red blood cell layer (lower layer) S3 ... Intermediate layer t1 ... First tube t2 ... Second tube.

──────────────────────────────────────────────────続 き Continued on the front page (58) Fields surveyed (Int. Cl. ⁷ , DB name) A61M 1/00-1/36 A61J 1/00-1/20

Claims (1)

(57) [Claims] 1. A component which is fractionated in at least three layers of an upper layer, an intermediate layer, and a lower layer is contained therein, and a first outlet and a second outlet are provided on one side of the upper layer or the lower layer. A container holder for holding a flexible parent container having an outlet, a support member for supporting one of two opposing surfaces of the parent container held by the container holder, and the other surface A liquid separating device having a container pressing portion comprising: a pressing member configured to press the first layer and the intermediate layer; and a first partitioning unit configured to partition the upper layer and the intermediate layer; A second partitioning means provided between the first partitioning means and the second partitioning means;
An interface detection unit that detects an interface of the intermediate layer; and the pressing member includes a first pressing member, a second pressing member, and a third pressing member adjacent to the first and second pressing members. When the first outlet and the second outlet are on the side of the upper layer, the first pressing member presses the upper layer partitioned by the first partitioning means, and the upper layer is The first outlet is guided by the second pressing member to press the lower layer partitioned by the second partitioning means, and the third pressing member is used to press the first outlet and the second layer.
A contact portion is formed on two opposing surfaces of the parent container at least across the intermediate layer and the lower layer from between the outlets, and the contact portion communicates with the second outlet in the parent container. Forming a passageway to press the lower layer, leading the lower layer to the second outlet through the passageway, wherein the first outlet and the second outlet are on the side of the lower layer; The second pressing member presses the lower layer partitioned by the second partitioning device, and guides the lower layer to the second outlet, and the first pressing member allows the first partitioning device to set the lower layer. While pressing the partitioned upper layer, the third outlet member and the second outlet are pressed by the third pressing member.
Between the outlets, at least across the intermediate layer to the upper layer, to form contact portions on two opposing surfaces of the parent container, and the contact portions communicate with the first outlet in the parent container. A liquid separating apparatus, wherein a passage is formed to press the upper layer, and the upper layer is led to the first outlet through the passage.