JPH06225935A - Liquid separator - Google Patents

Abstract

PURPOSE:To provide a liquid separator with higher separation accuracy by enabling the separation of liquids separated into at least three layers to fluids for each of the layers without requiring any complicated control from a master container in which the liquids are sealed. CONSTITUTION:This liquid separator feeds a fluid in an upper layer S1 and a fluid in the lower layer S2 by pressurizing a master bag B1. It has a first protruded part 61 to divide the upper layer S1 and an intermediate layer S3, a second protruded part 62 to divide the lower layer S2 and the intermediate layer S3, an interface detection member to detect an interface of the intermediate layer S3 and a press piece 75 to divide and form an internal path D.

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a liquid separating device for taking out each layer of liquid from a flexible container in which liquids separated into a plurality of layers such as a blood bag are sealed.

[0002]

2. Description of the Related Art Recently, instead of whole blood transfusion, component transfusion in which only necessary components in blood are transfused to a patient or the like, and only plasma is collected from blood for preparation of plasma preparation. Has also been done. In order to obtain such blood for component transfusion and plasma preparation, a flexible blood bag, so-called multi-bag, has been used. This blood bag is composed of a blood collection bag (so-called parent bag) for containing blood collected from a donor, and one or more separation bags (so-called child bags) connected to the parent bag via a tube. The blood components in the constructed and centrifuged parent bag are taken out for each component and taken into the child bag.

When a bag for collecting blood is placed in a centrifuge, blood is a plasma layer, an erythrocyte layer, and an intermediate layer (a buffy coat layer containing platelets, white blood cells or fat, and other bacteria, so-called buffy coat layer). However, in order to use the separated components later, it is necessary to separate and collect each component from the blood collection bag so that they do not mix with each other. .

A liquid separating device is required in addition to the blood bag in order to carry out such separate collection, and there are blood bags shown in FIGS. 10 to 12. Figure 10
In the blood bag 100A shown in (1), a tube is attached to the upper part of the parent bag 101 that stores the collected blood. Tubes t1 to t3 are connected to this tube via two T-shaped branch tubes, and a child bag 102a containing a red blood cell preservation solution at the tip of each tube t1 to t3.
And two empty child bags 102b and 102c are connected.

To separate each component from the parent bag 101, first, the abdominal surface of the parent bag 101 containing the blood separated into the three layers of the plasma layer S1, the red blood cell layer S2 and the intermediate layer S3 by centrifugation. The blood plasma component forming the upper layer S1 is transferred to the child bag 102b through the tube t2 by pressurizing. When the transfer of the plasma component is completed, a small amount of the plasma component remaining in the parent bag 101, the component of the intermediate layer, that is, the buffy coat layer, and a part of the red blood cell component of the lower layer are transferred to the child bag 102c via the tube t3. Transfer. When the intermediate layer is removed from the parent bag 101 in this manner, the red blood cell preservation solution contained in the child bag 102a is then transferred through the tube t1 to the parent bag 101.
Transfer inside. The blood bag 100A for separating the components of blood in this manner is practical because the components can be collected from one direction (upper side in this example) of the parent bag 101.

A blood bag 100B shown in FIG. 11 has tubes t1 and t2 provided above and below a parent bag 101. The parent bag 101 is in a state of being separated into three layers, that is, the plasma layer S1, the red blood cell layer S2, and the intermediate layer S3 by centrifugation. By applying pressure from the upper tube t1, the plasma layer S1 is taken out to the child bag 102a, the red blood cell layer S2 is taken out from the lower tube t2 to the child bag 102b, and the intermediate layer S3 is left in the parent bag 101. It was done (Japanese Patent Publication Sho 6)
3-20, 144). This blood bag 100
Since the desired components of B are taken out from the centrifugally separated state, the intermediate layer can be efficiently separated and collected from the blood. The reference numeral “103” in FIGS. 10 and 11 is a blood collection needle.

In the blood bag 100C shown in FIG. 12, a seal portion 104 extending to the bottom of the bag is formed by heat-sealing the front and back surfaces of the parent bag 101, thereby forming a passage 105 for taking out upward in the bag. It was formed independently. Then, the centrifugally separated parent bag 10
1 is attached to the liquid separation device and pressurized to
Of the plasma layer S1 from the tube t1 of the second tube t
The erythrocyte layer S2 is taken out from No. 2 and the intermediate layer S3 is left in the parent bag 101 (see European Patent Publication No. 0448751A1). This blood bag 100C is practical because each component can be taken out from above.

On the other hand, in the liquid separating device, the centrifugally separated parent bag 101 is pressurized to flow the plasma layer S1 toward the first child bag 102a and the red blood cell layer S2 toward the second child bag 102b. A container pressurizing unit is provided. The container pressurizing unit conventionally has a lower pressure biasing method as shown in FIG. 13 depending on the method of pressurizing the parent bag (for example, see Japanese Patent Publication No. 55-17,585).
14 and a parallel pressurizing method (see, for example, JP-A-55-155,652). The former is one in which the parent bag 101 is hung on the hook 36 provided on the separation stand 106, and the pressure plate 107 whose lower end is rotatably supported pressurizes the belly surface of the bag 101, and the latter is separated. A pressure plate 108 is provided in parallel with the stand 106, and the abdominal surface of the bag 101 is pressed in this parallel state. These include automatic pressurization and manual pressurization, but in the most frequently used automatic pressurization, both methods detect the position of the intermediate layer S3 by an interface sensor (not shown). The parent bag 101 is pressurized by the pressure plate 107 or 108.

As shown in FIGS. 11 and 12, the liquid separating apparatus has clamp members 40a, 40 for individually and independently interrupting the flow of fluid flowing through the tubes t1, t2.
b are provided, and these clamp members 40a, 40
In b, the intermediate layer S3 detected by the interface sensor is the tube t.
It is designed such that it is individually opened / closed so that it is not taken in at 1 or t2.

For example, when the pressure in the parent bag 101 is continued and the intermediate layer S3 is displaced upward by more than the allowable dimension from the initial position when centrifugally separated, the clamp member 40a for the tube t1 is driven to be closed. Then, the tube t1 is closed and the removal of the plasma layer S1 is temporarily stopped. Then
When only the erythrocyte layer S2 is taken out and the intermediate layer S3 is lowered to a position within the allowable range due to the pressurization of the parent bag 101, the clamp member 40a is driven to open and the taking out of the plasma layer S1 is restarted.

[0011]

However, in the case of taking out the intermediate layer S3 from the parent bag 101, such as the blood bag 100A shown in FIG. 11, the components of this intermediate layer S3 are deposited on the inner wall of the parent bag 100A. Easy to remain, the intermediate layer S
It was difficult to completely remove 3 from the parent bag 101.

Further, in the conventional liquid separating apparatus, the open / close drive control of the clamp members 40a, 40b and the parent bag 1 are performed while tracking and detecting the constantly changing position of the intermediate layer S3.
Since the pressure control of 01 has to be performed, there is a drawback that the control configuration of the entire apparatus becomes complicated.

The present invention has been made in order to solve the problems associated with the above-mentioned prior art, and from a parent container in which a liquid separated into at least three layers is enclosed, without complicated control. It is an object of the present invention to provide a liquid separation device having high separation accuracy, which can be separated into fluid for each layer.

[0014]

According to the present invention to achieve the above object, the components fractionated in at least three layers of an upper layer, an intermediate layer and a lower layer are housed inside, and the upper layer or the lower layer is contained. Of a container holding part for holding a flexible parent container having a first outlet and a second outlet on one side, and two facing surfaces of the parent container held by the container holding part Of 1
A liquid separating device comprising: a container pressing portion including a supporting member that supports one surface and a pressing member that presses the other surface, which is a first device for partitioning the upper layer and the intermediate layer.
Partitioning means, second partitioning means for partitioning the intermediate layer and the lower layer, and interface detection provided between the first partitioning means and the second partitioning means for detecting an interface of the intermediate layer Means and the pressing member include a first pressing member, a second pressing member, and a third pressing member adjacent to the first and second pressing members.
When the first outlet and the second outlet are on the side of the upper layer, the first pressing member pushes the upper layer partitioned by the first partitioning means. Leading the upper layer to the first outlet, pressing the lower layer partitioned by the second partitioning means by the second pressing member, and pressing the first outlet by the third pressing member. Adhesive portions are formed on two opposite surfaces of the parent container from between the second outlets and at least across the intermediate layer to the lower layer, and the second outlets are formed in the parent container by the adhesive portions. A passage communicating with the lower layer is pressed to guide the lower layer to the second outlet through the passage, and the first outlet and the second outlet are on the lower layer side. If there is, the second pressing member causes the second partitioning means to The partitioned lower layer is pressed to guide the lower layer to the second outlet, and the first pressing member presses the upper layer partitioned by the first partitioning means, and 3 The pressing member opposes the parent container 2 between the first outlet and the second outlet, at least across the intermediate layer to the upper layer.
A close contact portion is formed on the surface, and the close contact portion forms a passage communicating with the first outlet in the parent container, presses the upper layer, and connects the upper layer to the upper layer through the passage. It is a liquid separation device characterized in that it is led to the outlet of No. 1.

The present invention is also characterized in that it has a weight measuring section for always measuring the weight of the parent container.

[0016]

According to the present invention described in claim 1, the first and second
Since the intermediate layer is partitioned from other layers by the partitioning means of
The position of the intermediate layer does not change even when the parent container is being pressurized, that is, when the fluid of the other layer is flowing out. Therefore,
Since there is no risk that the intermediate layer will flow out of the parent container, a fluid separation that does not cause the intermediate layer to flow out of the parent container is performed.
Control during this separation work is also easy.

Further, when the first outlet and the second outlet are on the upper layer side, the third pressing member defines a passage for guiding the fluid of the lower layer to the second outlet in the parent container. If the first outlet and the second outlet are on the side of the lower layer, the passage for guiding the fluid of the upper layer to the first outlet can be defined in the parent container. Therefore, a plurality of fluid take-out tubes can be connected to one end of the parent container to take out the components of the fluid from one direction, and the fluid take-out tubes can be handled easily.

Further, even when the interface position of the intermediate layer is different for each parent container, the intermediate layer can be partitioned from the other layers by the first and second partitioning means, and the intermediate layer flows out from the parent container. A fluid separation that does not occur is performed.

[0019]

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS An embodiment of the present invention will be described below with reference to the drawings. 1 is a schematic perspective view of a liquid separation device according to an embodiment of the present invention, FIG. 2 is a schematic plan view showing an example of a blood bag, and FIG. 3 is a schematic sectional view taken along line 3-3 of FIG. , Fig. 4
1 is a horizontal plane cross-sectional view showing the main part of FIG. 1, FIG. 5 is a side view showing the container pressurizing unit in a partial cross section, and FIG. 6 is a perspective view showing the main part of the container pressurizing unit shown in FIG. , Fig. 7 (1)
(4) is an explanatory view showing a pressurizing operation of the container pressurizing unit.

In FIG. 1, a liquid separating apparatus 10 is to take out component blood from a centrifuged blood bag B for each component blood, and selectively holds a container holding portion 11 holding the blood bag B and tubes t1 and t2. Clamping member 1 to clamp
2, a container pressurizing unit 13 for pressurizing the parent bag B1 held by the container holder 11, and the container pressurizing unit 1
And a control unit C that controls the third and the like.

The blood bag B is made of a flexible synthetic resin, and as shown in FIG. 2, a blood collection bag containing blood collected from a donor, a so-called parent bag B1 (corresponding to a parent container), The parent bag B1 is composed of a separation bag, so-called a child bag B2a and a child bag B2b, which are connected to each other via first and second tubes t1 and t2. A blood bag B in which a plurality of child bags B2a ..., B2b ... Are connected to each of the first and second tubes t1 and t2.
There is also.

At the top of the container of the parent bag B1, a first port Q1 (corresponding to the first outlet) to which a first fluid take-out tube t1 is connected and a second port to which a second fluid take-out tube t2 is connected Q2 (corresponding to the second exit) is provided. A partition wall d is formed on the parent bag B1 so as to partition the first port Q1 and the second port Q2 by heat-sealing the front and back surfaces of the bag. The partition wall d of this embodiment does not extend to the bottom of the bag like the seal portion 104 shown in FIG. An internal passage D is formed by the partition wall d and a pressing piece 75 provided on the container pressurizing means 18 described later. When the blood in the parent bag B1 is centrifuged, the plasma layer S
1 (corresponding to the upper layer), erythrocyte layer S2 (corresponding to the lower layer) and intermediate layer S3 are separated into three layers, but the plasma layer S1 and the erythrocyte layer S2 are used. The component blood of the plasma layer S1 is taken out from the parent bag B1 through the first port Q1 and further taken into the first child bag B2a through the first tube t1. Further, the component blood of the red blood cell layer S2 is guided to the second port Q2 through the internal passage D, taken out from the second port Q2, and further the second tube t.
2 is taken into the second child bag B2b into which the red blood cell preservative solution has been previously injected. Incidentally, when the red blood cell layer S2 flows out through the internal passage D, the intermediate layer S3 may be mixed to some extent, but according to experiments, it has been found that there is no problem.

As shown in FIGS. 1 and 3, the container pressurizing unit 13 is provided in a housing 15 installed in the front part of the main body case 14, and the front wall 1 of the housing 15 is provided.
A pair of hooks 11 is projectingly provided on 5a. A through hole 17 opened in the parent bag B1 is hooked on the hook 11 to serve as a container holding portion for hanging the parent bag B1.

Housing 15 of container pressurizing unit 13
Is attached to the aluminum block 45 and the housing 1
Inside the container 5, as shown in detail in FIG.
From the back side of the parent bag B1 suspended from the bag B1
A pressure block 60 that can be pressed against 1 and a container pressing means 18 that pressurizes the parent bag B1 are provided.

As shown in FIG. 6, the pressure block 60 has a substantially convex shape and is arranged so that its convex tip end faces the back surface of the parent bag B1.
It is provided so as to be movable back and forth along the horizontal direction from the back side to the abdominal side and vertically movable along the vertical direction of the parent bag B1. A first convex portion 61 (corresponding to a first partitioning means) for partitioning the plasma layer S1 and the intermediate layer S3 by pressing the parent bag B1 against the support plate 19 which will be described later at the tip end of the pressure block 60. And the support plate 19
A second convex portion 62 (corresponding to a second partitioning means) which presses the parent bag B1 between it and to partition the red blood cell layer S2 and the intermediate layer S3 is formed vertically with a predetermined gap. . The weight of the intermediate layer S3 is usually 40 to 50 g or less, but in order to eliminate the possibility that the fluid of the intermediate layer S3 is taken into the child bags B2a and B2b, the gap between the convex portions 61 and 62 is 80.
The size is set to contain about 150 g.

A groove 63 having a predetermined depth is provided between the convex portions 61 and 62 so that a part of the parent bag B1 can escape when the parent bag B1 is pressed. Also,
An interface detecting member 43 (corresponding to interface detecting means) for detecting the interface of the intermediate layer S3 is provided between the groove 63, that is, between the convex portions 61 and 62. As the interface detection member 43, a photosensor generally composed of a light emitting element and a light receiving element is used, and the interface is detected by the difference in the light absorption rate of each of the three layers described above. The signal from the member 43 is input to the control unit C. Further, the groove 63 may be provided with a suction member for preventing the intermediate layer S3 from spreading in the vertical direction.

A guide pin 65 that extends horizontally toward the support plate 19 is attached to a holding plate 64 that is vertically movable in the housing 15. A guide hole 67 that extends horizontally and is slidably fitted into the guide pin 65 is formed in the leg portion 66 of the pressure block 60 which is located in the vertical direction. A pinion gear 68 attached to a holding plate 64 is meshed with a rack 69 attached to the pressure block 60, and a motor (not shown) is connected to the pinion gear 68. By rotating the pinion gear 68 in the clockwise direction in FIG. 5, the pressure block 60 moves forward toward the support plate 19 while being guided by the guide pin 65. On the contrary, the pinion gear 68
5 is rotated counterclockwise in FIG. 5, the pressure block 60 moves backward in a direction away from the support plate 19. When the pressure block 60 moves to the forward limit position, the first convex portion 61 and the second convex portion 62
Support plate 19 through the back and abdomen of parent bag B1
Press against.

The container pressurizing means 18 (corresponding to a container pressing portion) is in contact with the abdominal surface side of the parent bag B1 and is in a stationary state when pressurized, and the parent bag. A first pressure plate 20a (corresponding to a first pressing member) and a second pressure plate 20b (corresponding to a second pressing member) that are in contact with the back side of B1 and the first and second pressing plates. Plates 20a and 20b are parent bags B1
And driving members 21a and 21b that apply pressure toward the support plate 19 side.

In this embodiment, the support plate 19 is composed of a door body that opens and closes the front surface of the housing 15. The door body is preferably made of a transparent material such as acrylic resin so that the state of the parent bag B1 inside can be seen. By using the door body as the support plate 19 without providing an independent support member, the number of parts can be reduced and the configuration can be simplified.

First and second pressure plates 20a, 20b
As shown in FIG. 6, the base end portion is rotatably supported by the support shafts 23a and 23b attached to the first and second convex portions 61 and 62, respectively.

The drive members 21a and 21b are provided with a housing 70 attached to the leg portion 66 of the pressure block 60.
A screw portion 71 rotatably held in the housing 70, a nut portion 72 movably attached to the screw portion 71, and a motor 7 for rotating the screw portion 71.
3 and 3. The screw portion 71 and the nut portion 72 are formed of a ball screw, and the nut portion 72 is attached to the housing 70 so as to move in the horizontal direction in the drawing by the rotation of the screw portion 71. The nut portion 72 and the first and second pressure plates 20a and 20b are connected via a connecting rod 74 whose both ends are rotatably supported. By rotating the screw portion 71 in an appropriate direction by the motor 73, the nut portion 7
2 moves in the horizontal direction, and along with the movement of the nut portion 72, the first and second pressure plates 20a, 20b move toward the support plate 19 side with the support shafts 23a, 23b as the center, or It rotates in the direction away from 19.

The container pressing means 18 further includes a parent bag B1.
And an internal passage forming means for partitioning and forming an internal passage D (corresponding to a passage) for guiding the fluid in the lower layer, that is, the red blood cell layer S2, to the second port Q2 in the parent bag B1. In this embodiment, the internal passage forming means is, as shown in FIGS. 5 and 6, a pressure block 6
Pressing piece 7 attached to one side of No. 0 and extending vertically
5 (corresponding to the third pressing member).
Since the pressing piece 75 is provided at the tip of the pressure block 60, as the first convex portion 61 and the second convex portion 62 of the pressure block 60 are pressed against the parent bag B1, the pressing piece 75 is attached to the parent bag B1. Press against. When the pressing piece 75 is pressed against the parent bag B1, the pressing piece 75 extends from the lower end of the partition wall d formed on the parent bag B1 to the vicinity of the bottom of the bag B1 as shown by the phantom line in FIG. In the expanded state, the internal passage D for guiding the fluid of the red blood cell layer S2 to the second port Q2 is defined in the parent bag B1.

As shown in FIG. 2, the pressing piece 75 extends vertically at a position closer to the center of the bag by a certain distance from the side of the parent bag B1 on the second port Q2 side. Moreover, the 1st convex part 61 and the 2nd convex part 62 are extended horizontally from the pressing piece 75 toward the other end side of a bag. still,
At the lower end of the pressing piece 75, the pressing piece 75 has the first and second convex portions 6
When moving up and down in conjunction with the first and second parts 62, it is sufficient that at least the second convex part 62 on the lower side extends. Further, the pressing piece 75 does not necessarily have to be moved up and down. In the case of the parent bag B1 having the partition wall d as shown in FIG. 2, the pressing piece 75 does not have to reach the upper end of the parent bag B1.
In addition, as shown in FIG.
May be formed, and in this case, the pressing piece 7
5 reaches the upper end of the parent bag B1. The present invention can also be applied to such a parent bag B1. In this case, not only the production of the parent bag B1 becomes easier due to the abolition of the partition wall d, but also the liquid separating apparatus 10 can be manufactured. When the parent bag B1 is attached, it can be attached without being aware of the front and back of the parent bag B1, and the attachment work is extremely easy.

As shown in FIG. 5, the container pressurizing unit 13 further includes the pressure block 60, the container pressurizing means 18 and the pressing piece 75 attached to the pressure block 60, and the intermediate layer S3 detected by the interface detecting member 43. It has a moving means 76 for moving along the vertical direction according to the interface position. More specifically, in the housing 15 of the container pressing unit 13,
A base plate 77 that supports the holding plate 64 so as to be vertically movable is attached, and the upper and lower ends of the base plate 77 are bent to form a screw holding portion 78. Further, the holding plate 64 also has its upper and lower ends bent to form a nut holding portion 79. A screw portion 80 is rotatably held between the screw holding portions 78 of the base plate 77, and a nut portion 81 attached to the screw portion 80 is attached to a nut holding portion 79 of the holding plate 64. A motor 82 for rotationally driving the screw portion 80 is attached to the upper screw holding portion 78,
Further, the screw portion 80 and the nut portion 81 are composed of ball screws. The holding plate 64 is rotated by the rotation of the screw portion 80.
In order to vertically move the base plate 77, a long hole 84 is formed in the base plate 77 along which the guide portion 83 connected to the nut holding portion 79 of the holding plate 64 slides.

The controller C first drives the motor 82 to move the holding plate 64 up and down, and then the interface detecting member 43.
From the intermediate layer S3 based on this signal.
The interface position of is detected. Next, the control unit C controls the intermediate layer S
In order to position 3 between the first convex portion 61 and the second convex portion 62, the holding plate 64 is moved upward, or
Determine whether to move downward. Based on this judgment, the control section C outputs a control signal for rotating the motor 82 in the proper forward / reverse directions to the motor 82, and the intermediate layer S3 is formed approximately in the middle between the convex portions 61 and 62. When it detects that it is located, it outputs a stop signal to the motor 82.

The upper portion of the main body case 14 is shown in FIG.
As shown in FIGS. 3 and 4, a tray 42 for accommodating the child bags B2a and B2b is formed. The tray 42 is closed by an upper lid 47, hermetically sealed by a surrounding packing 48, and the inside of the tray 42 is brought into a depressurized state by a pump P and a guide tube 49. This is to make it easier for the component blood to flow from the parent bag B1 into the child bags B2a and B2b.

Further, as shown in FIGS. 1 and 4, in the upper part of the main body case 14, the flow of the component blood flowing in the two tubes t1 and t2 extended from one end of the parent bag B1. A clamp member 12 is provided for individually opening and blocking. The clamp member 12 is composed of a first clamp member 40a that opens and blocks the tube t1 through which the blood plasma component S1 flows, and a second clamp member 40b that opens and blocks the tube t2 through which the red blood cell component S2 flows. And these clamp members 4
It has solenoids 41a and 41b for operating 0a and 40b. A signal is sent from a control unit (not shown) to the solenoids 41a and 41b, and based on this signal, the opening / closing control of the clamp members 40a and 40b is independently performed. There is. For each component blood S1 and S2, from the parent bag B1 to the child bag B
When the removal to 2a, B2b is completed, both clamp members 4
Each of the tubes t1 and t2 is blocked by 0a and 40b. In the liquid separating apparatus 10 of this embodiment, the intermediate layer S3 is formed by the both convex portions 61 and 62 of the pressure block 60.
Is pre-partitioned from the upper layer S1 and the lower layer S2, the opening / closing control of each clamp member 40a, 40b is not performed in order not to change the position of the intermediate layer S3.

Incidentally, reference numeral "G" in the figure is a weight measuring portion provided for continuously measuring the weight of B1 of the parent bag,
"44" is a weight sensor including a strain gauge attached to an aluminum block 45 provided so as to support the housing 15 of the container pressing unit 13, and "46" is a scale stopper. By measuring the weight of B1 of the parent bag held in the container holding section 11 by the weight measuring section G, it is judged whether the parent bag B1 is suitable or not, and by continuously measuring the weight, the inside of the parent bag B1 is judged. The remaining amount of is always detected. Then, when the remaining weight in the parent bag B1 measured by the weight measuring unit G reaches a predetermined weight during the liquid separation, the operation of the container pressurizing means 18 is stopped, and further, the clamp member 40a, 40b causes the tube t1. , T2 are cut off, and the withdrawal of liquid from the first and second ports Q1, Q2 is stopped.

Next, the operating state of this embodiment will be outlined.

First, by the pressing piece 75 as the third pressing member, the lower layer is cut across the intermediate layer S3 from between the first and second ports Q1 and Q2 located on the upper side between the two opposing surfaces of the parent container. A close contact portion reaching S2 is formed, and the close contact portion forms an internal passage D in the parent bag B1 for connecting the lower layer S2 and the second port Q2. And the first
The component of the upper layer S2 partitioned from the intermediate layer S3 by the first convex portion 61 as the partitioning means is taken out from the first port Q1 by being pressed by the first pressure plate 20a as the first pressing member. . On the other hand, the component of the lower layer S2 partitioned from the intermediate layer S3 by the second convex portion 62 serving as the second partition means is pressed by the second pressure plate 20b serving as the second pressing member, so that the internal passage It is led to the upper second port Q2 through D and taken out from the second port Q2. That is, the components of the lower layer S2 are also taken out from above.

The above is the outline of the operation of this embodiment.
The details will be described with reference to (1) to (4).

First, as shown in FIG. 7A, the blood bag B is set in the housing 15 of the container pressurizing unit 13. Here, the parent bag B1 has already been centrifuged by the centrifuge, and the blood inside is separated into three layers S1, S2 and S3. The set of the bag B is such that the through hole 17 of the parent bag B1 is hooked on the hook 11 and the child bags B2a and B2b connected to the tubes t1 and t2 extended from the parent bag B1 are placed in the tray 42 and then the upper lid is set. It is done by closing 47. Moreover, the support plate 19 which is a door is closed.

Next, as shown in FIG. 7 (2), the motor 82 of the moving means 76 is driven to move the holding plate 64 up and down, and the interface position of the intermediate layer S3 is determined based on the signal from the interface detection member 43. To detect. After that, the motor 82 is rotationally driven in the forward and reverse proper directions according to the interface position of the intermediate layer S3, and the intermediate layer S3 is positioned substantially in the middle between the first convex portion 61 and the second convex portion 62 of the pressure block 60. Then, the motor is stopped.

Then, the pinion gear 68 is driven in the clockwise direction in FIG. 5, and the pressure block 60 is moved forward toward the support plate 19 while being guided by the guide pin 65. When the pressure block 60 moves to the forward limit position, as shown in FIG. 7C, the first convex portion 61 and the second convex portion 62 are pressed against the support plate 19 via the back surface and the abdominal surface of the parent bag B1. The intermediate layer S3 is enclosed between the convex portions 61 and 62. That is, the plasma layer S1 and the intermediate layer S3 are partitioned by the first convex portion 61, and the red blood cell layer S2 and the intermediate layer S3 are partitioned by the second convex portion 62. Further, as the pressure block 60 is pressed against the parent bag B1, the pressing piece 75 as the pressure-contacting internal passage forming means is pressed against the parent bag B1, and by this pressing piece 75 and the partition wall d formed on the parent bag B1. , An internal passage D for guiding the fluid of the red blood cell layer S2 to the second port Q2 is defined in the parent bag B1.

When the containment of the intermediate layer S3 is completed, FIG.
As shown in (4), the drive member 21 of the container pressing means 18
The motor 73 provided in a and 21b is driven, and each of the first and second pressure plates 20a and 20b is connected to the support shaft 23.
It rotates about a and 23b in the direction toward the support plate 19 side. First and second pressure plates 20a, 20b
With this rotation, the parent bag B1 is attached to the support plate 19 and the first and second pressure plates 20a, 2a, 2
As a result, the plasma layer S1 in the parent bag B1 is sent to the child bag B2a through the first port Q1 and the first tube t1. The red blood cell layer S2 is
It is sent to the child bag B2b through the internal passage D, the second port Q2 and the second tube t2.

After a predetermined time has passed, the driving member 2
Each of the motors 1a and 21b is reversely driven simultaneously or individually to rotate each of the first and second pressure plates 20a and 20b in a direction away from the support plate 19 about the support shafts 23a and 23b. . With this rotation, the pressurization of the parent bag B1 is released, and the outflow of the component blood from the plasma layer S1 and the red blood cell layer S2 is stopped. In addition, the intermediate layer S
3 That is, the buffy coat layer remains in the parent bag B1.

When each component blood is taken out from the parent bag B1 into the child bags B2a and B2b in this manner, the tubes t1 and t2 are closed by a clip or the like, and the pinion gear 68 is used.
Is driven counterclockwise in FIG. 5 to move the pressure block 60 backward in a direction away from the support plate 19. Further, open the door body 19, and the parent bag B1 and child bag B
When 2a and B2b are removed from the liquid separating apparatus 10, a series of operations for separating blood into each component blood is completed.

In the liquid separating apparatus 10 of this embodiment, before the parent bag B1 is pressurized, the convex portions 61 and 62 as the partitioning means are provided.
Thus, since the intermediate layer S3 is partitioned from the other layers S1 and S2, the position of the intermediate layer S3 does not change during the pressurization of the parent bag B1, that is, the outflow of the component blood. For this reason,
The control becomes extremely easy as compared with the case where the opening / closing drive control of the clamp member and the pressurization control of the parent bag B1 are performed while tracking and detecting the position of the intermediate layer S3 which constantly changes. Further, since the intermediate layer S3 is partitioned from the other layers S1 and S2, the intermediate layer S3 does not flow out from the parent bag B1 and it is possible to perform blood separation with high separation accuracy.

Further, in this embodiment, since the pressing piece 75 is provided as the internal passage forming means for partitioning and forming the internal passage D for guiding the fluid of the lower red blood cell layer S2 to the second port Q2 in the parent bag B1. The parent bag B1 can be provided with two ports Q1 and Q2 on the top thereof, and when the parent bag B1 is attached to the liquid separating apparatus 10, the tube t1,
The handling of t2 becomes easy.

Further, since the moving means 76 for moving the pressure block 60, the container pressurizing means 18, etc. along the vertical direction according to the interface position of the intermediate layer S3 is provided, the intermediate layer S
Even if the interface position of 3 is different for each parent bag B1, blood can be separated with high accuracy.

It should be noted that the present invention is not limited to the above-mentioned embodiments, but can be appropriately modified and used within the scope of the claims. For example, in the above-described embodiment, a blood bag is used to separately collect each component blood,
The present invention can be used not only for this but also for the fractional collection of various liquids. Further, although the case of separating the liquid separated into three layers has been described, the present invention is applied by increasing the partitioning means also to the parent container in which the liquid separated into four layers or more is enclosed. It is possible.

In the above-described embodiment, the case where both the first and second convex portions 61 and 62 as the first and second partitioning means are provided in one pressure block 60 has been described.
The partitioning means can be pressed individually against the parent bag B1.
It may be provided so as to be vertically movable. Container pressurizing means 18
Also, the present invention is not limited to the illustrated example, and may have a pressure plate of a parallel pressure type, for example.

Further, although the case where the first and second ports Q1 and Q2 are on the upper layer S1 side is illustrated, the present invention shows that the first and second ports Q1 and Q2 are on the lower layer S1 as shown in FIG. It is also applied to the case on the S2 side, that is, when the parent bag B1 is held by the container holding portion 11 with the first and second ports Q1 and Q2 to which the tubes t1 and t2 are connected being positioned on the lower side. can do. In this case, the pressing piece 75 as the third pressing member crosses at least the intermediate layer S3 from between the first and second ports Q1 and Q2 located on the lower side between the two facing surfaces of the parent container. As a result, a close contact portion reaching the upper layer S1 is formed, and the close contact portion forms an internal passage D in the parent bag B1 for connecting the upper layer S1 and the first port Q1. Then, the component of the lower layer S2 partitioned from the intermediate layer S3 by the second convex portion 62 serving as the second partitioning means is pressed by the second pressure plate 20b serving as the second pressing member, so that the second port Q2
Taken out. On the other hand, the component of the upper layer S1 partitioned from the intermediate layer S3 by the first convex portion 61 serving as the first partitioning means is pressed by the first pressure plate 20a serving as the first pressing member, so that the internal passage It is guided to the lower first port Q1 through D and taken out from the first port Q1. That is, the components of the upper layer S1 are also taken out from below.

[0054]

As described above, according to the present invention, since the intermediate layer is partitioned from the other layers by the first and second partitioning means, the parent container is under pressure, that is, the other layer. The position of the intermediate layer does not change even when the fluid is flowing out. Therefore, since there is no possibility that the intermediate layer will flow out of the parent container, it is possible to perform fluid separation with high separation accuracy without causing the intermediate layer to flow out of the parent container without complicated control.

Further, since the third pressing member is provided, when the first outlet and the second outlet are on the upper layer side, a passage for guiding the fluid of the lower layer to the second outlet is defined in the parent container. When the first outlet and the second outlet are on the side of the lower layer, the passage for guiding the fluid of the upper layer to the first outlet can be defined and formed in the parent container. By connecting a plurality of fluid extraction tubes to one end of the parent container, the components of the fluid can be extracted from one direction, and the fluid extraction tubes can be handled easily.

Further, even when the interface position of the intermediate layer is different for each parent container, the intermediate layer can be partitioned from the other layers by the first and second partitioning means, and the intermediate layer flows out from the parent container. It becomes possible to perform separation of the fluid without causing it.

[Brief description of drawings]

FIG. 1 is a schematic perspective view of a liquid separation device according to an embodiment of the present invention,

FIG. 2 is a schematic plan view showing an example of a blood bag,

FIG. 3 is a schematic cross-sectional view taken along line 3-3 of FIG.

4 is a horizontal cross-sectional view showing the main part of FIG.

FIG. 5 is a schematic side view showing the container pressurizing unit in a partial cross section.

6 is a perspective view showing a main part of the container pressurizing unit shown in FIG.

7 (1) to 7 (4) are explanatory views showing a pressurizing operation of the container pressurizing unit.

FIG. 8 is a schematic plan view showing a blood bag according to another embodiment,

FIG. 9 is a schematic plan view showing a blood bag according to still another embodiment.

FIG. 10 is a front view showing an example of a conventional blood bag,

FIG. 11 is a schematic front view showing another example of a conventional blood bag,

FIG. 12 is a schematic front view showing still another example of a conventional blood bag,

FIG. 13 is a schematic explanatory view showing an example of a conventional liquid separation device.

FIG. 14 is a schematic explanatory view showing another example of a conventional liquid separation device.

[Explanation of symbols]

DESCRIPTION OF SYMBOLS 11 ... Hook (container holding part) 13 ... Container pressurizing unit 18 ... Container pressurizing means (container pressing part) 19 ... Support plate (supporting member) 20a ... 1st pressurizing plate (1st pressing member) 20b ... 2nd Pressure plate (second pressing member) 43 ... Interface detecting member (interface detecting means) 61 ... First convex part (first partitioning means) 62 ... Second convex part (second partitioning means) 75 ... Pressing piece (third part) Pressing member) B ... container B1 ... parent bag (parent container) B2a ... first child container B2b ... second child container D ... internal passage Q1 ... first port (first outlet) Q2 ... second port (second) Outlet) S1 ... Plasma layer (upper layer) S2 ... Red blood cell layer (lower layer) S3 ... Intermediate layer t1 ... First tube t2 ... Second tube.

[Procedure amendment]

[Submission date] March 18, 1993

[Procedure Amendment 1]

[Document name to be corrected] Drawing

[Correction target item name] All drawings

[Correction method] Change

[Correction content]

[Figure 1]

[Fig. 2]

[Figure 3]

[Figure 4]

[Figure 10]

[Fig. 13]

FIG. 14

[Figure 5]

[Figure 6]

[Figure 7]

[Figure 8]

[Figure 9]

FIG. 11

[Fig. 12]

Claims (1)

[Claims] 1. A composition containing fractionated components in at least three layers of an upper layer, an intermediate layer and a lower layer, wherein the first outlet and the second layer are provided on one side of the upper layer or the lower layer. A container holding part for holding a flexible parent container provided with an outlet, a support member for supporting one of two facing surfaces of the parent container held by the container holding part, and the other face Is a liquid separating device having a container pressing portion formed of a pressing member that presses the intermediate layer, the first partitioning means for partitioning the upper layer and the intermediate layer, and the intermediate layer and the lower layer. Second partitioning means for performing, and provided between the first partitioning means and the second partitioning means,
An interface detection unit that detects an interface of the intermediate layer, the pressing member includes a first pressing member, a second pressing member, and a third pressing member adjacent to the first and second pressing members, and When the first outlet and the second outlet are on the side of the upper layer, the first pressing member presses the upper layer partitioned by the first partitioning means to move the upper layer to the upper layer. Leading to a first outlet, pressing the lower layer partitioned by the second partitioning means by the second pressing member, and pressing the first outlet and the second layer by the third pressing member.
Forming a close contact portion on the two opposing surfaces of the parent container from at least the crossing of the intermediate layer to the lower layer from between the outlets, and the close contact portion communicates with the second outlet in the parent container. Forming a passage to press the lower layer, leading to the lower layer through the passage to the second outlet, where the first outlet and the second outlet are on the lower layer side; , The second pressing member presses the lower layer partitioned by the second partitioning means, guides the lower layer to the second outlet, and the first pressing member creates the first partitioning means. While pressing the partitioned upper layer, the third pressing member causes the first outlet and the second
From at least one of the outlets across at least the intermediate layer to the upper layer, forming a close contact portion on the two opposing surfaces of the parent container, and the close contact portion communicates with the first outlet in the parent container. A liquid separation device, characterized in that a passage is formed to press the upper layer, and the upper layer is led to the first outlet through the passage.