JPH06218039A - Liquid separator - Google Patents

Abstract

PURPOSE:To provide a liquid separator which is versatile and has very high separation accuracy by taking out of liquid components from above to improve controllability along with a simple handling of a tube. CONSTITUTION:A blood bag (B) is pressed by a pressure-contact means (X1) having a plate-shaped pressure-contact member (33) and by an opening/closing clamp means (X2) from the outside to form a passage (D) in the blood bag (B) and then, the blood bag (B) is pressed by a depressing means (17). Thus, liquids (S1, S2 and S3) separated centrifugally in a plurality of layers within the blood bag (B) are taken out from a plurality of ports (31 and 32) provided at the top part of the blood bag (B).

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a liquid separating device for taking out liquids of respective layers from a flexible container in which liquids separated into a plurality of layers are sealed, such as a blood bag.

[0002]

2. Description of the Related Art Recently, instead of whole blood transfusion, component transfusion in which only necessary components in blood are transfused to a patient or the like, and only plasma is collected from blood for preparation of plasma preparation. Has also been done. In order to obtain such blood for component transfusion and plasma preparation, a flexible blood bag, so-called multi-bag, has been used. This blood bag is a blood collection bag (so-called parent bag) in which blood collected from a donor is placed, and one or more separation bags connected to the parent bag via a fluid extraction tube (hereinafter simply referred to as a tube). (So-called child bag), the blood component in the centrifuged parent bag is taken out for each component and taken into the child bag.

When a bag for collecting blood is placed in a centrifuge, blood is a plasma layer, an erythrocyte layer, and an intermediate layer (a buffy coat layer containing platelets, white blood cells or fat, and other bacteria, so-called buffy coat layer). However, in order to use the separated components later, it is necessary to separate and collect each component from the blood collection bag so that they do not mix with each other. .

A liquid separating device is required in addition to the blood bag in order to carry out such separation and collection, and there are blood bags shown in FIGS. 9 to 11. In blood bag 100A shown in FIG. 9, a tube is attached to an upper portion of parent bag 101 that stores the collected blood.
Tubes t1 to t3 are connected to this tube via two T-shaped branch tubes, and a tip end of each tube t1 to t3 contains a child bag 102a containing a red blood cell preservation solution, and two empty child bags 102b, 102c is connected. To separate each component from the parent bag 101, first, the abdominal surface of the parent bag 101 containing the blood separated into the three layers of the plasma layer S1, the red blood cell layer S2, and the intermediate layer S3 by centrifugation is pressurized. As described above, the plasma component forming the upper layer S1 is passed through the tube t2 to the child bag 102.
Transfer to b. When the transfer of the plasma component is completed, a small amount of the plasma component remaining in the parent bag 101, the component of the intermediate layer, that is, the buffy coat layer, and a part of the red blood cell component of the lower layer are transferred to the child bag 102c via the tube t3. Transfer. When the intermediate layer is removed from the parent bag 101 in this way, the red blood cell preservation solution contained in the child bag 102a is then transferred into the parent bag 101 via the tube t1. The blood bag 100A for separating the components of blood in this manner is practical because the components can be collected from one direction (upper side in this example) of the parent bag 101.

A blood bag 100B shown in FIG. 10 has tubes t1 and t2 provided above and below a parent bag 101.
The parent bag 101, which has been separated into the three layers by centrifugation, is attached to the liquid separation device, and the plasma layer S1 is taken out from the upper tube t1 into the child bag 102a by applying pressure in the same manner as described above, and then downward. The erythrocyte layer S2 is taken out from the tube t2 in the child bag 102b, and the intermediate layer S3 is left in the parent bag 101 (see Japanese Patent Publication No. 63-20,144). In this blood bag 100B, desired components are taken up and down from the centrifugally separated state, so that the intermediate layer can be efficiently separated and collected from the blood. The reference numeral “103” in FIGS. 9 and 10 is a blood collection needle.

In the blood bag 100C shown in FIG. 11, a partition 104 extending to the bottom of the bag is formed by heat-sealing the front and back surfaces of the parent bag 101, thereby forming a flow path 105 for taking out upward in the bag. It was formed independently. Then, the centrifuged parent bag 101
Is attached to a liquid separation device, and by pressurizing the plasma layer S1 from the first tube t1 and the second tube t2.
The erythrocyte layer S2 is taken out from the container, and the intermediate layer S3 is left in the parent bag 101 (see European Patent Publication No. 04841751A1). This blood bag 100C is practical because each component can be taken out from above.

[0007]

However, as in the blood bag 100A shown in FIG. 9, the intermediate layer S3 is used for the child bag 1.
In the case of the blood bag 100c, the intermediate layer S3 is likely to remain on the inner wall of the parent bag 10, and the timing of taking out the intermediate layer S3 is difficult, which causes a problem in controllability. In the case where the tubes t1 and t2 are provided above and below, the component extraction ports are located above and below, so that handling of the tubes t1 and t2 becomes inconvenient when the bag is attached to the blood component separation device.

In the parent bag 101 shown in FIG. 11, after the centrifugal separation, since the plasma component and the intermediate layer component are present in the formed flow path 105 in addition to the lower layer red blood cell component, the accuracy of collecting the red blood cell component is It will decrease in proportion to the width of the path 105. In order to improve accuracy, narrowing the channel width
Not only it takes a long time to take out, but also the blood flow velocity flowing through the flow path becomes extremely high, which may destroy red blood cells, which is not preferable.

Further, if a certain width of the flow path is secured, the accuracy of collecting red blood cell components becomes low, and after separation and collection, there is a possibility that the human body may be affected by use. Therefore, leukocyte removal filters and various adsorbents are used. Then, it becomes necessary to remove such impure components before use.

Further, the liquid separating device only pressurizes the bag to discharge the blood inside the bag to the outside, and there is one that changes the shape or configuration of the bag itself to ensure or assist the discharge of blood. It is not provided with versatility.

The present invention has been made in order to solve the problems associated with the above-mentioned prior art. The structure of the parent container is changed by pressing the parent container with an L-shaped pressure member so that the parent container does not have a problem. An object of the present invention is to provide a versatile liquid separation device which can easily take out a fluid in a desired layer and can take out liquid only from an upward direction, and which is easy to handle tubes and has a simple controllability. And

[0012]

The present invention which achieves the above object has at least three internal layers, namely, an upper layer, an intermediate layer and a lower layer.
A container holding part that holds the fractionated components in one layer and holds a flexible parent container having a first outlet and a second outlet on one side of the upper layer or the lower layer; Liquid separation having a container pressurizing unit made up of a support member that supports one of two facing surfaces of the parent container held by the container holding unit and a pressurizing member that pressurizes the other surface A device, wherein the pressure member comprises a substantially L-shaped pressure member and a second pressure member provided adjacent to the L-shaped pressure member,
While maintaining the three regions of the intermediate layer and the lower layer, the parent container across the intermediate layer from between the first outlet and the second outlet to a predetermined distance before the other end of the parent container Of the independent chamber of the large capacity communicating with the first outlet in the parent container and the small capacity communicating with the second outlet in the parent container. By forming an independent chamber of a part and rotating an L-shaped pressure member that is rotatably supported about a direction crossing the intermediate layer, the L-shaped pressure member in the direction intersecting the axis is released. And a driving means for driving the L-shaped pressure member so as to form a passage between the large capacity portion and the small capacity portion, and the second pressure member presses the large capacity portion, The component of the larger volume adjacent to the passage is transferred to the second output. A liquid separation device, characterized in that leads to.

[0013]

The L-type pressure contact means presses the parent container in the vertical direction to form a flow path for leading out a desired layer portion from the second outlet. A desired layer can be easily derived by forming the flow path. Further, since the liquid can be taken out from above the parent container, controllability and handleability are excellent, and since no unnecessary components are mixed in, liquid component separation with extremely high separation accuracy can be achieved.

[0014]

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS An embodiment of the present invention will be described below with reference to the drawings.

FIG. 1 is a schematic perspective view of a liquid separating apparatus according to an embodiment of the present invention, FIGS. 2 to 4 are schematic plan views each illustrating a blood bag, and FIG. 5 is a schematic view taken along line 5-5 of FIG. FIG. 6 is a cross-sectional view, FIG. 6 is a perspective view showing a pressurizing operation of the press separation unit,
FIG. 7 is a schematic horizontal cross-sectional view of FIG.

In FIG. 1, a liquid separating apparatus 10 takes out each blood component from a blood collection bag (hereinafter also simply referred to as a parent bag) B1 obtained by centrifuging a blood bag, and holds the parent bag B1. Two tubes connected to the container holding portion 11 via first and second ports 31 and 32 (corresponding to a first outlet and a second outlet) provided at the top of the parent bag B1. A clamp member 12 for selectively clamping t1 and t2, and the tube t
Two separation bags (hereinafter, also simply referred to as child bags) B2 (collectively referred to as child bags B2a and B2b) are connected to the other ends of 1 and t2 through tubes t1 and t2, and further held in the container holding portion 11. It has a pressing and separating unit 13 (corresponding to a container pressing unit) for pressing the formed parent bag B1 and a control unit C for controlling each component of the pressing and separating unit 13.

The blood bag B is made of a flexible synthetic resin, and as shown in FIG. 2, a parent bag B1 for containing blood collected from a donor and a tube t for the parent bag B1.
It is composed of two child bags B2a and B2b which are communicated with each other through 1. Further, in FIG. 2, a seal portion d having a distal end extending in the longitudinal direction downward from between the first port 31 and the second port 32 provided on the top of the parent bag B1 is provided in the middle of the parent bag B1. I am Further, in FIG. 3, the parent bag B1 is not provided with the seal d, and in FIG. 4, the parent bag B1 is provided with a seal portion d and a point seal portion d'provided on an extension of the end of the seal portion d. Show what you have.

When the blood in the parent bag B1 is centrifuged,
It is separated into a plasma layer S1 (corresponding to the upper layer), a red blood cell layer S2 (corresponding to the lower layer) and an intermediate layer S3, but it is the plasma layer S1 and the red blood cell layer S2 that are used. , The plasma layer S1 is taken out from the parent bag B1 via the tube t1, is housed in the empty child bag B2a, the red blood cell layer S2 is taken out via the tube t2, and the red blood cell preservative solution for preventing the coagulation of red blood cells is contained. It is accommodated in the child bag B2b.

As shown in FIGS. 1 and 5, the pressing / separating unit 13 is provided in a housing 15 provided in the front part of a main body case 14, and a hook-shaped container is provided on a front wall 15a of the housing 15. The holding portion 11 is projected,
The through hole 1 formed in the parent bag B1 in the container holding portion 11
6 is hung and the parent bag B1 is hung.

Housing 15 of pressure separating unit 13
Is mounted on an aluminum block 45, and as shown in FIG. 5, the parent bag B1 hung on the container holding portion 11 is pressed from the abdominal surface side and the back surface side of the parent bag B1 so that the plasma layer S1 component is first A bag pressing portion 17 which constitutes a pressing means is provided so that the red blood cell layer S2 component can be led out from the first port 31 through the second port 31, respectively. Bag pressing part 1
7 includes a first pressing plate 18 (corresponding to a supporting member) that is in contact with the ventral side of the parent bag B1 and is in a stationary state when pressed, and a second pressing plate 19 (additional member that is in contact with the back side of the parent bag B1 B). Corresponding to the second pressure member of the pressure member),
The direction in which the second pressing plate 19 presses the parent bag B1,
That is, it has a spring member 20 that applies pressure toward the first pressing plate 18 side.

In this embodiment, the first pressing plate 18 is
It is composed of a door body that opens and closes the front portion of the housing 15, and is inscribed in the parent bag B1 when the door body is closed. The first pressing plate 18 is preferably made of a transparent material such as acrylic resin so that the state of the parent bag B1 inside can be visually observed.

On the other hand, the second pressing plate 19 is provided inside the housing 15 so as to face the first pressing plate 18, and the first link 22 is provided on the back side of the second pressing plate 19. An end of a second link 24, which is rotatably supported by a support shaft 23 attached to the housing 15, is connected to a rear end of the first link 22 so as to be movable.

Particularly, in this embodiment, as shown in FIGS. 5 and 6, a pressure contact unit X1 and an opening / closing clamp unit X2 are provided in the pressure separating unit 13 inside the housing 15.

The pressure contact means X1 is pressure-contacted from the abdominal surface side and the back surface side of the parent bag B1 to a position on the extension of the end of the seal portion d provided in the parent bag B1 to press the red blood cell layer S2 component from the second port 32. It is composed of a plate-like pressure contact member 33 that forms a flow path D that leads out, a front-rear movement block 35 for moving the pressure contact member 33 in the front-rear direction, and a cylinder member 37 that operates the front-rear movement block 35. .

The opening / closing clamp means X2 is a plate-shaped press-contact closing member 34 integrally attached to the plate-like press-contact member 33, and is connected to the lower surface of the press-contact closing member 34 and has a forward / backward moving block 35 inserted therein. Swivel axis 38 extended
And a shaft turning member 39 such as a rotary solenoid attached to the end of the turning shaft 38.

The pressure contact member 33 and the pressure contact closing member 3
Reference numeral 4 denotes a plate member integrally formed so as to have an inverted L-shape and an L-shape (hereinafter also simply referred to as a substantially L-shape member), and a portion of the pressure-contact member 33 that is pressed against the parent bag B1. The surface shape is preferably a pointed shape so that a sufficient pressing force is applied and the formed flow path D and other parts can be completely sealed.

The substantially L-shaped member corresponds to the L-shaped pressing member of the pressing member. Further, the cylinder member 37 and the shaft rotating member 39 correspond to driving means, and the substantially L-shaped member driven by this driving means operates as follows. First, in the substantially L-shaped member, a contact portion where the abdominal surface and the back surface of the parent bag B1 which face each other are in contact with each other is defined by the plasma layer S1, the red blood cell layer S2,
While maintaining the three regions of the intermediate layer S3, the first port 3
It is formed between the first and second ports 31 across the intermediate layer S3 up to a predetermined distance before the lower end of the parent bag B1. At the same time, the close contact portion causes the first port 31 to be placed in the parent bag B1.
To form an independent chamber having a large capacity communicating with the second port 32 and an independent chamber having a small capacity communicating with the second port 32. The substantially L-shaped member is rotatably supported about a direction (vertical direction in the drawing) that traverses the intermediate layer S3, and by rotating the substantially L-shaped member by the driving means, the pressure contact closing member 34 is formed. By releasing (the L-shaped pressing member in the direction intersecting the axis), a passage is formed between the large capacity portion and the small capacity portion. The second pressing plate 19 is a parent bag B1.
Is pressed to guide the component of the large capacity portion adjacent to the flow path D to the second port 32.

The position (height) of the pressure contact closing member 34 is not particularly limited, but from the viewpoint of collecting blood components having extremely high separation accuracy, the lower end position is the red blood cell layer. It is preferably located within S2. With this configuration, the flow path D is formed by the substantially L-shaped member.
When the intermediate layer S3 is formed, the plasma layer S1 component can be immediately taken out from the first port 31 without being mixed with the intermediate layer S3 as an impurity, and when the intermediate layer S3 rises beyond the inlet of the channel D for a while. The pressure blocking member 34 can be opened to remove the red blood cell layer S2 component from the second port.
The intermediate layer S3 is not mixed in the components of the plasma layer S1 and the red blood cell layer S2.

Further, by taking out the plasma layer S1 component and the erythrocyte layer S2 component alternately at regular intervals, it is possible to minimize the amount of vertical movement of the intermediate layer S3. S3 is plasma layer S1, red blood cell layer S2
It is possible to eliminate the risk of contamination with the above and to improve the detection accuracy of the interface detection member 43 described later.

The pressing means X1 may move to the seal portion d of the parent bag B1 while the parent bag B1 is being pressed. In this case, it is necessary to attach a horizontal moving unit that supports the entire pressure contact means X1 and open / close clamp means X2 on the substrate and moves the substrate by moving means (not shown). As a result, the pressure contact member 33 of the substantially L-shaped member is moved from the side end of the parent bag B1 to the first and second members.
It is possible to move the parent bag B1 horizontally in the horizontal direction while pressing it vertically between the ports, and as a result, a new flow path D can be formed in the parent bag B1 from the outside by the substantially L-shaped member. it can. In this case, the position (height) of the press contact closing member 34 described above is not particularly limited.

As shown in FIGS. 1 and 5, the upper portion of the main body case 14 has the first and second ports 3 of the parent bag B1.
A clamp member 12 is provided for individually controlling the cutoff-release of the flow of the blood component flowing in the two tubes t1 and t2 connected to 1, 32. As shown in FIG. 2, the clamp member 12 includes a first clamp member 40a that opens and blocks the tube t1 through which the plasma component flows.
A second clamp member 40b for opening and blocking the tube t2 through which the red blood cell component flows, and these clamp members 40
a and 40b for operating solenoids 41a and 41b, and the position of the intermediate layer S3 of the centrifuged blood,
The shut-off control is performed by a signal from the control unit C which is issued according to the weight of the parent bag B1 and the pressurizing time. The blood components that have flowed out through the tubes t1 and t2 are child bags B2 (B2a, B2) installed in a tray 42 formed in the upper part of the main body case 14.
b) It is stored in.

The position of the intermediate layer S3 is detected by the interface detecting member 43 provided on the second pressing plate 19.
A photosensor is generally used as the interface detection member 43, and the interface detection member 43 detects the interface based on the difference in the light absorptance of each of the three layers described above.
The signal from 3 is input to the control unit C.

The weight of the parent bag B1 is measured by the weight measuring section G.
Is a weight sensor 4 at the bottom of the pressure separating unit 13.
4 is provided. The weight sensor 44 can be configured by, for example, a strain gauge attached to a side surface of a block 45 provided to support the housing 15 of the pressing and separating unit 13. Then, in order to measure the weight of the parent bag B1, the total weight of the pressing and separating unit 13 is used to determine the housing 15 and the first and second pressing plates 18 and 1.
It is calculated by subtracting the tare of 9 or the like.

The tray 42 is closed by an upper lid 47, is hermetically sealed by a packing 48 around the tray 42, and has a negative pressure inside by a pump P and a guide tube 49. This is to facilitate the inflow of blood components from the parent bag B into the child bag B2.

The pressurizing time is detected by the timer 51 provided on the control panel 50 provided on the front surface of the main body case 14, and the signal from the timer 51 is also input to the control section.

Next, the operation of this embodiment will be described.

First, after setting the blood bag B in the housing 15 of the container pressurizing unit 13, when the power of the control unit C is turned on and the program is started, the memory is initialized and the weight measuring unit and the pressure measuring unit are It is initialized. Then, if you enter a predetermined operation mode etc.,
The clamp member is in the "open" state.

Here, the parent bag B1 has already been centrifuged by the centrifuge, and the blood inside is separated into three layers. The set of bags is the parent bag B
The through hole 16 of 1 is hung on the container holding portion 11, the tubes t1 and t2 extended from the parent bag B1 are fitted into the clamp members 40a and 40b, respectively, and the child bag B2 (B2
After placing a, B2b) in the tray 42, the upper lid 47 is closed.

Then, the start switch is turned on. If the start switch is turned on, the tubes t1 and t2 are normally set, the weight of the parent bag B1 is also a predetermined weight, the vacuum pump P is activated, and the pressure inside the tray 42 is reduced, The clamp members 40a and 40b are closed by the solenoids 41a and 41b. When the tubes t1 and t2 are not properly set, or when the weight of the parent bag B1 is not a predetermined weight, both clamp members 40a and 40b do not operate.

If the parent bag B1 is normal, the start switch is turned on again. The weight of the parent bag B1 is calculated by subtracting the tare of the housing 15 and the first and second pressing plates 18 and 19 from the total weight of the pressing and separating unit 13. That is, the weight is detected by subtracting the weight of the tare of the pressure separation unit 13 previously input to the control unit C from the weight of the pressure separation unit 13 detected by the weight sensor 44. If this weight is a predetermined weight, the door is closed. When the weight of the parent bag B1 does not reach the predetermined weight, the parent bag B1 is removed and removed as a nonconforming item.

In the parent bag B1 thus weight-checked, the interface position (initial position) of the intermediate layer S3 is detected by the interface sensor 43. If both are normal,
The timer 51 starts and the operation is displayed. In this case, by rotating the handle 28 after closing the door body, the parent bag B1 is pressed by the substantially L-shaped members provided on the abdominal surface side and the rear surface side by the pressure contacting means X1 and the opening / closing clamp means X2. . The press-contact means X1 moves the pair of plate-like press-contact members 33 to a position on the extension of the end of the seal portion d provided on the parent bag B1, and the cylinder member 37 is actuated to move the press-contact members X1 through the front-rear moving block 35. 33 moves forward and presses the parent bag B1 from the abdominal surface side and the back surface side. As a result, the flow path D for leading out the red blood cell layer S2 component from the second port 32 is formed. In this case, the opening / closing clamp means X
Since the second pressure contact closing member 34 is also pressure contacted from the abdominal surface side and the back surface side of the parent bag B1, the lower end position of the pressure contacting member 33 is at the plasma layer S.
It is preferably located within 1.

In this way, the flow path D for deriving the red blood cell layer S2 component by pressing the abdominal surface side and the back surface side of the parent bag B1 from the outside in this way.
Therefore, no matter what kind of container or where the intermediate layer S3 is present in the parent bag B1, the desired layer D can be easily derived by forming the desired flow path D. You will be able to do it.

While the parent bag B1 is pressure-contacted by the substantially L-shaped members of the pressure-contact member 33 and the pressure-contact closing member 34, which are integrally provided with each other, from the side end of the parent bag B1 to the first port 31 and the first port 31. When it moves horizontally to the position between the two ports 32, the flow path D becomes closed, and the liquid is pushed out from the flow path, and the liquid component does not exist. Therefore, it is not desirable for the intermediate layer B3 to flow out to the outside.
It becomes possible to form the flow path D while eliminating the above, and if the pressure contact closure by the opening / closing clamp means X2 is released, the first port 31 and the second port 3 can be easily formed depending on the position of the intermediate layer B3.
It is possible to separately collect the plasma layer S1 and the red blood cell layer S2 from 2.

When the pressing by the first and second pressing plates 18, 19 is started following the formation of the flow path, the position of the intermediate layer S3 is always detected by the interface detecting member 43.

When the measurement of the interface detecting member 43 is within a preset position (for example, the position where the intermediate layer S3 is applied to the upper end of the parent bag B or the lower end of the flow path D), both clamp members are measured. The open / closed state of 40a and 40b is continued. However, when the position of the intermediate layer S3 exceeds the set position, the clamp member 40a of the tube t1 through which the plasma layer S1 flows or the clamp member 40b of the tube t2 through which the red blood cell layer S2 flows is closed. In this way, the intermediate layer S3 has a child bag B2a rather than the parent bag B1.
Blood is separated while controlling the clamp member 12 so as not to flow out to B2b.

After a lapse of a predetermined time, both clamp members 40a and 40b are closed and the extraction of blood components is stopped.

In this way, when each blood component is taken out from the parent bag B1 to the child bag B2, the tubes t1 and
The t2 is closed with a clip or the like, the door 21 is opened, and the parent bag B1 and child bag B2 are removed from the blood component separation device.

The present invention is not limited to the above-mentioned embodiments, but can be variously modified within the scope of the claims. For example, the above-described embodiment uses a blood bag to separately collect blood components, but the present invention can be applied to not only this but also various liquid components. The pressure contact means may be not only one of the parent containers but also pressure contact from both sides, and the pressure contact member and the opening / closing clamp means do not necessarily have to be integrally formed.

Further, as shown in FIG.
The parent bag B1 may be held by the container holding portion 11 with the ports 31 and 32 to which 1, t2 are connected being positioned on the lower side. The pressure contact closing member 34 in this case is the pressure contact member 33.
Is provided at the upper end of the channel, and in this holding state, the flow path D in which the component of the upper layer S1 is formed by the substantially L-shaped member
And is taken out from the lower side of the parent bag B1.

[0050]

As described above, according to the liquid separating apparatus of the present invention, the L-type pressurizing member presses the parent container in the vertical direction to form the flow path for discharging the component from the predetermined outlet. The flow path is pressure-closed, and then the parent container is pressed by the second pressure member to take out the predetermined component from the predetermined outlet. Subsequently, the intermediate layer is located above the flow path position formed by the L-shaped pressure member. After moving, the pressure contact blockage of the flow path is released to allow other components to be taken out from the other outlet, which makes it possible to take out the required liquid component from above the parent container, which is excellent in controllability and handleability. In addition, since the unnecessary components are not mixed in at all, the separation precision is extremely high, and the blood component separation is versatile and can be used in various bags.

Further, as in the liquid separating apparatus of the present invention, by using the L-shaped pressure member which comes into pressure contact with the parent container from the front-rear direction, wrinkles cannot be formed on the parent container and a smooth and highly accurate separation operation can be performed. Is.

[Brief description of drawings]

FIG. 1 is a schematic perspective view showing a liquid separation device according to an embodiment of the present invention,

FIG. 2 is a schematic plan view showing an example of a blood bag that can be used in the above embodiment,

FIG. 3 is a schematic plan view showing another example of the blood bag,

FIG. 4 is a schematic plan view showing still another example of the blood bag,

5 is a schematic sectional view taken along line 5-5 of FIG.

FIG. 6 is a perspective view showing a pressurizing operation of the press separation unit,

FIG. 7 is a schematic horizontal cross-sectional view of FIG.

FIG. 8 is a front view showing a parent bag held by another holding method.

FIG. 9 is a schematic plan view showing an example of a conventional blood bag,

FIG. 10 is a schematic plan view showing another example of a conventional blood bag,

FIG. 11 is a schematic plan view showing still another example of a conventional blood bag.

[Explanation of Codes] 11 ... Container Holding Section 12 ... Clamping Member, 13 ... Press Separation Unit (Container Pressurizing Section)
Back pressing portion, 18 First pressing plate (supporting member), 19 Second pressing plate 19 (second pressing member of pressing member) 31 First port (first outlet) 32 Second Ports (second outlets), 33, 34 ... L-shaped members (L-shaped pressing members) (33 ... Pressure contact members, 34 ... Pressure contact closing members) 37, 39 ... Driving means, 43 ... Interface detection member, B … Blood bag, B1… Parent bag (parent container), B2 (B2a,
B2b) ... Child bag, C ... Control part, D ... Flow path, d ... Seal part, d '... Point seal part, G ... Weight measuring part, S1
... Plasma layer (upper layer), S2 ... Red blood cell layer (lower layer), S3
... intermediate layer, t1, t2 ... fluid take-out tube, X1 ... pressure contact means, X2 ... open / close clamp means.

[Procedure amendment]

[Submission date] March 18, 1993

[Procedure Amendment 1]

[Document name to be corrected] Drawing

[Correction target item name] All drawings

[Correction method] Change

[Correction content]

[Figure 1]

[Fig. 2]

[Figure 3]

[Figure 4]

[Figure 5]

[Figure 7]

[Figure 8]

[Figure 9]

[Figure 6]

[Figure 10]

FIG. 11

Claims (1)

[Claims] 1. A composition containing fractionated components in at least three layers of an upper layer, an intermediate layer and a lower layer, wherein the first outlet and the second layer are provided on one side of the upper layer or the lower layer. A container holding part for holding a flexible parent container provided with an outlet, a support member for supporting one of two facing surfaces of the parent container held by the container holding part, and the other face A liquid separating apparatus comprising: a container pressurizing unit configured to pressurize a container, the pressurizing member including a substantially L-shaped pressurizing member and a second pressurizing member provided adjacent to the L-shaped pressurizing member. Of the other end of the parent container across the intermediate layer from between the first outlet and the second outlet while maintaining the three regions of the upper layer, the intermediate layer and the lower layer. A close contact portion is formed on two opposing surfaces of the parent container up to a predetermined distance, and the close contact portion is formed. To form a separate chamber of a small portion of the volume which communicates with the separate chambers of a large portion of the capacity in communication with the first outlet in the parent vessel to said second outlet,
By rotating the L-shaped pressure member that is rotatably supported about the axis crossing the intermediate layer, the L-shaped pressure member in the direction intersecting with the axis is released and the large volume portion and the small volume portion are released. Drive means for driving the L-shaped pressurizing member so as to form a passage therebetween, and the second pressurizing member presses the portion having a large capacity, and the portion having a large capacity adjacent to the passage. Is introduced into the second outlet.