JP2912489B2 - Skin cosmetics - Google Patents
Skin cosmeticsInfo
- Publication number
- JP2912489B2 JP2912489B2 JP274192A JP274192A JP2912489B2 JP 2912489 B2 JP2912489 B2 JP 2912489B2 JP 274192 A JP274192 A JP 274192A JP 274192 A JP274192 A JP 274192A JP 2912489 B2 JP2912489 B2 JP 2912489B2
- Authority
- JP
- Japan
- Prior art keywords
- skin
- lupeol
- present
- good
- effect
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Landscapes
- Cosmetics (AREA)
Description
【０００１】[0001]
【産業上の利用分野】本発明は、例えば、表皮細胞の増
殖、分化の促進、角質層のターンオーバー促進、角質改
善、美肌効果などの皮膚老化防止効果に優れた皮膚化粧
料に関する。BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a skin cosmetic which is excellent in skin aging prevention effects such as promotion of epidermal cell proliferation and differentiation, promotion of horny layer turnover, improvement of keratin and beautiful skin effect.
【０００２】[0002]
【従来の技術および課題】老化皮膚では、一般に、表皮
および真皮の細胞活性が低下し、そのため、老化皮膚の
肌は角質層の機能低下により乾燥肌または真皮線維芽細
胞の機能低下により弾力性のない肌となる。このような
老化皮膚の活性を回復させるために、細胞賦活剤につい
ての研究がなされ、真皮線維芽細胞に対する増殖促進作
用のあるプラセンタエキス、乳精抽出物などの物質が提
案されている。しかし、表皮細胞の増殖や分化を促進す
る物質およびその使用に関する報告は、ほとんどなされ
ていないのが現状である。BACKGROUND OF THE INVENTION In aging skin, the cell activity of the epidermis and dermis is generally reduced, so that the skin of the aging skin has elasticity due to reduced function of the stratum corneum and dry skin or dermis fibroblasts. No skin. In order to restore the activity of such aged skin, studies on cell activators have been made, and substances such as placenta extract and whey extract which have a growth promoting action on dermal fibroblasts have been proposed. However, at present, there are few reports on substances that promote proliferation and differentiation of epidermal cells and their use.
【０００３】[0003]
【課題を解決するための手段】そこで本発明者らは、表
皮細胞の増殖および分化を促進する賦活剤を得るべく鋭
意研究した結果、トリテルペンの一種であるルペオール
およびその誘導体が表皮細胞の増殖を顕著に促進する作
用を有することを見いだした。その後、さらに鋭意研究
を重ねた結果、該化合物が角質層の機能を改善し、さら
に、ルペオール類を配合してなる皮膚化粧料が老化皮膚
のターンオーバーを亢進し、肌あれ改善効果、角質改善
効果に対して顕著な作用を呈すること、また、しっとり
感、なめらか感、張り、艶を皮膚に付与する美肌効果を
も発現することが判明した。The inventors of the present invention have conducted intensive studies to obtain an activator that promotes the proliferation and differentiation of epidermal cells. As a result, lupeol, a kind of triterpene, and its derivatives increase the proliferation of epidermal cells. It has been found to have a remarkable promoting effect. Thereafter, as a result of further intensive studies, the compound improved the function of the stratum corneum, and further, a skin cosmetic containing lupeols accelerated the turnover of aging skin, improved skin roughness, and improved keratin. It has been found that it has a remarkable effect on the effect, and also exhibits a beautiful skin effect of giving the skin a moist feeling, a smooth feeling, firmness, and luster.
【０００４】すなわち、本発明は、ルペオールおよび／
またはその有機酸エステル誘導体を含有することを特徴
とする皮膚老化防止効果の優れた皮膚化粧料を提供する
ものである。That is, the present invention provides lupeol and / or lupeol.
Another object of the present invention is to provide a skin cosmetic composition having an effect of preventing skin aging characterized by containing an organic acid ester derivative thereof.
【０００５】本発明に用いるルペオールはα−およびβ
−体が存在することが知られており、例えば、クワ科、
トウダイグサ科、キョウチクトウ科、ヤマモモ科、キク
科、フトモモ科、アオギリ科、カキノキ科、マメ科等の
植物に広く存在する物質である。また、そのアセチル誘
導体等の有機酸エステル誘導体も種々知られておりルペ
オールを常法に従ってアルカノイル化することにより得
られる。本発明においては、これらのルペオールまたは
その誘導体は単独で、あるいは２種以上を組合せて用い
ることができる。[0005] Lupeol used in the present invention comprises α- and β-
-The body is known to exist, for example, mulberry,
It is a substance that is widely present in plants such as Euphorbiaceae, Apocynaceae, Bayberry, Asteraceae, Myrtaceae, Aomoriaceae, Antrodiaceae, and Leguminosae. Various organic acid ester derivatives such as acetyl derivatives are also known, and can be obtained by alkanoylation of lupeol according to a conventional method. In the present invention, these lupeols or derivatives thereof can be used alone or in combination of two or more.
【０００６】これらルペオールまたはその誘導体は、皮
膚機能を亢進し、皮膚が本来備えている機能を修復ある
いは改善して皮膚を健常な状態に保ち、特に、老化皮膚
に適用する場合、著しい効果が認められる。[0006] These lupeols or derivatives thereof enhance the skin function, restore or improve the function originally provided by the skin and keep the skin healthy, and especially when applied to aging skin, a remarkable effect is recognized. Can be
【０００７】本発明においては、ルペオールまたはその
誘導体を、ルペオールとして皮膚化粧料全量に対して
０．００１〜２．０重量％配合することが好ましい。よ
り好ましくは、０．０１〜１．０重量％である。かかる
配合量が０．００１％未満であると所望の効果が十分発
揮されない。一方、２．０重量％を超えてもその増加分
に相当する効果の向上は得られない。In the present invention, it is preferred that lupeol or a derivative thereof is incorporated as lupeol in an amount of 0.001 to 2.0% by weight based on the total amount of the skin cosmetic. More preferably, it is 0.01 to 1.0% by weight. If the amount is less than 0.001%, the desired effect cannot be sufficiently exhibited. On the other hand, even if the content exceeds 2.0% by weight, the effect corresponding to the increase cannot be improved.
【０００８】本発明の皮膚化粧料は、常法に従って、例
えば、ローション類、乳液類、クリーム類、パック類等
の剤形とすることができる。また、本発明の化粧料には
その種類に応じ、性能を損なわない範囲において適宜公
知の化粧品用成分、例えば、色素、香料、防腐剤、界面
活性剤、顔料、抗酸化剤等を配合することができる。[0008] The skin cosmetic of the present invention can be made into dosage forms such as lotions, emulsions, creams, packs and the like in accordance with a conventional method. In addition, the cosmetics of the present invention may optionally contain known cosmetic ingredients such as pigments, fragrances, preservatives, surfactants, pigments, antioxidants, and the like, as long as the performance is not impaired, depending on the type of the cosmetics. Can be.
【０００９】[0009]
【実施例】以下、試験例および実施例を挙げて本発明を
さらに詳細に説明する。 角化細胞増殖促進試験 培養細胞 角化細胞には培養細胞として確立されているＳＶ４０ト
ランスフォームヒトケラチノサイトを用いた。EXAMPLES Hereinafter, the present invention will be described in more detail with reference to Test Examples and Examples. Keratinocyte proliferation promotion test Cultured cells As the keratinocytes, SV40 transform human keratinocytes which have been established as cultured cells were used.
【００１０】試験方法 クリーンベンチ内において、滅菌した牛胎仔血清２．５
ｍｌ、ケラチノサイト基本培地７５ｍｌ、抗生物質０．
８ｍｌを入れ、そこに１．６×１０5個の角化細胞を撤
き、６穴プレートに３ｍｌづつ分注して、炭酸ガス培養
器中５％の炭酸ガスを含有する雰囲気下３７℃で培養し
た。２４時間後に培養液を除去し、ケラチノサイト基本
培地１．５ｍｌ、ダルベッコ変法イーグル最少栄養培地
１．５ｍｌ、リノール酸５μg、脂肪酸フリー牛血清ア
ルブミン１００μg／ｍｌを入れ、試験物質を最終濃度
が０．１μｇ／ｍｌまたは１．０μｇ／ｍｌになるよう
に添加し、１週間培養した。Test method In a clean bench, sterilized fetal bovine serum 2.5
ml, keratinocyte basal medium 75 ml, antibiotic 0.
Add 8 ml, remove 1.6 × 10 5 keratinocytes therefrom, dispense 3 ml at a time into a 6-well plate, and in a carbon dioxide incubator at 37 ° C. in an atmosphere containing 5% carbon dioxide. Cultured. Twenty-four hours later, the culture solution was removed, and 1.5 ml of keratinocyte basic medium, 1.5 ml of Dulbecco's modified Eagle minimal nutrient medium, 5 μg of linoleic acid, and 100 μg / ml of fatty acid-free bovine serum albumin were added. It was added to 1 μg / ml or 1.0 μg / ml and cultured for one week.
【００１１】培養後、培養液を除去し、０．０２％ＥＤ
ＴＡ、次いで０．２５％トリプシンを含むダルベッコン
のリン酸緩衝液を加えて剥離した。次いで、これらを除
去し、各穴にダルベッコンのリン酸緩衝液１ｍｌを入
れ、懸濁させて血球計算盤で細胞数を計測した。After culturing, the culture solution is removed, and 0.02% ED
TA and then Dulbeccon's phosphate buffer containing 0.25% trypsin were added and detached. Then, these were removed, 1 ml of Dulbeccon's phosphate buffer was added to each well, suspended, and the number of cells was counted using a hemocytometer.
【００１２】試験結果 表１〜４に示す通り、ルペオール(α−およびβ−)およ
びそのアセチル誘導体はいずれも角化細胞の増殖を有意
に促進させることが認められた。Test Results As shown in Tables 1 to 4, it was confirmed that lupeol (α- and β-) and its acetyl derivative significantly promoted the proliferation of keratinocytes.
【００１３】[0013]
【表１】 [Table 1]
【００１４】[0014]
【表２】 [Table 2]
【００１５】[0015]
【表３】 [Table 3]
【００１６】[0016]
【表４】 [Table 4]
【００１７】美肌効果試験 試験方法 荒れ肌、小じわ、乾燥肌等を訴える女子被験者（３０〜
４０代）２０人に、後記実施例２のルペオールを０．５
重量％配合したクリーム（クリームＡ）、および対照の
ルペオール無配合のクリーム（クリームＢ）を１日２回
（朝、夕）連続３カ月塗布して３カ月後の効果を官能的
に評価した。試験結果を、皮膚の湿潤性、柔軟性、弾力
性および艶の各項目について５段階評価で表５に示す。
数値は各項目に対して回答した人数を示す。Skin effect test Test method Female subjects complaining of rough skin, fine wrinkles, dry skin, etc.
Forty people) add 20 lupeol of Example 2 to 20 people
The cream containing three percent by weight (cream A) and the control cream containing no lupeol (cream B) were applied twice a day (morning and evening) for three consecutive months, and the effect after three months was organoleptically evaluated. The test results are shown in Table 5 on a five-point scale for each item of skin wettability, flexibility, elasticity and gloss.
The numerical values indicate the number of respondents for each item.
【００１８】[0018]
【表５】 項目 評価 クリームＡ クリームＢ （本発明の化粧料） （対照） 非常に良い １０ ０ 良い ５ ２ 湿潤性 やや良い ４ ４ 変化なし １ １３ やや悪い ０ １ 非常に良い ９ ０ 良い ５ ２ 柔軟性 やや良い ５ ５ 変化なし １ １３ やや悪い ０ ０ 非常に良い ８ ０ 良い ５ １ 弾力性 やや良い ７ ４ 変化なし ０ １５ やや悪い ０ ０ 非常に良い ９ ０ 良い ６ １ 艶 やや良い ４ ３ 変化なし １ １５ やや悪い ０ １Table 5 Item Evaluation Cream A Cream B (Cosmetic of the present invention) (Control) Very good 100 Good 5 2 Wetability Somewhat good 4 4 No change 1 13 Somewhat bad 0 1 Very good 90 Good 5 2 Flexibility Good 5 5 No change 1 13 Somewhat bad 0 0 Very good 80 Good 5 1 Elasticity Somewhat good 7 4 No change 0 15 Somewhat bad 0 0 Very good 9 0 Good 61 Good Glossy Good 4 3 Change None 1 15 Somewhat bad 0 1
【００１９】試験結果 表５の結果から明らかなように、本発明のルペオール類
は、しっとり感、なめらか感、張り、艶を皮膚に付与す
る美肌効果を発現することが認められた。Test Results As is evident from the results in Table 5, it was confirmed that the lupeols of the present invention exhibited a beautiful skin effect of imparting a moist feeling, a smooth feeling, a firmness and a luster to the skin.
【００２０】次の実施例に具体的な本発明の化粧料の組
成を示す。 実施例１ 化粧水 成分 配合量(重量％) α−ルペオール ０．０５ グリセリン ６．０ エタノール ８．０ ポリオキシエチレン硬化ヒマシ油(６０Ｅ.Ｏ.) ０．８ パラオキシ安息香酸メチル ０．０５ クエン酸 ０．０５ クエン酸ナトリウム ０．０７ 香料 ０．１ 精製水 残部 精製水にグリセリン、クエン酸、クエン酸ナトリウムを
溶解する。別個にエタノールにα−ルペオール、ポリオ
キシエチレン硬化ヒマシ油（６０Ｅ.Ｏ.）、メチルパラ
ベン、香料を溶解し、前記の精製水溶液に加えて可溶化
し、濾過して化粧水を得た。The following examples show specific compositions of the cosmetics of the present invention. Example 1 Lotion Component Content (% by weight) α-Lupeol 0.05 Glycerin 6.0 Ethanol 8.0 Polyoxyethylene hydrogenated castor oil (60EO) 0.8 Methyl paraoxybenzoate 0.05 Citric acid 0.05 Sodium citrate 0.07 Fragrance 0.1 Purified water Rest Dissolve glycerin, citric acid and sodium citrate in purified water. Separately, α-lupeol, polyoxyethylene hydrogenated castor oil (60EO), methylparaben, and a fragrance were dissolved in ethanol, added to the purified aqueous solution, solubilized, and filtered to obtain a lotion.
【００２１】実施例２ クリーム 成分 配合量（重量％） 成分（Ａ） β−ルペオール ０．５ ステアリン酸アスコルビル １．０ サラシミツロウ ４．０ セタノール ２．０ ステアリン酸 １．０ ミリスチン酸イソプロピル ５．０ ラノリン ２．０ 流動パラフィン ９．０ 自己乳化型モノステアリン酸グリセリル ３．０ モノステアリン酸ポリオキシエチレンソルビタン(２０Ｅ.Ｏ.) １．５ パラオキシ安息香酸プロピル ０．１ 成分（Ｂ） パラオキシ安息香酸メチル ０．２ プロピレングリコール ５．０ 香料 ０．２ 精製水 残部Example 2 Cream Ingredients Blended amount (% by weight) Ingredient (A) β-lupeol 0.5 Ascorbyl stearate 1.0 Salami beeswax 4.0 Cetanol 2.0 Stearic acid 1.0 Isopropyl myristate 5.0 Lanolin 2.0 Liquid paraffin 9.0 Self-emulsifying glyceryl monostearate 3.0 Polyoxyethylene sorbitan monostearate (20EO) 1.5 Propyl parahydroxybenzoate 0.1 Component (B) Methyl paraoxybenzoate 0.2 Propylene glycol 5.0 Fragrance 0.2 Purified water Remainder
【００２２】クリームＡは、成分(Ａ)を加熱溶解して８
０℃に保持し、別に香料を除く成分(Ｂ)を加熱溶解して
８０℃に保ち、これに前記成分(Ａ)を撹拌しながら加
え、冷却を行い、香料を加え、さらに冷却して得た。ク
リームＢの対照は、前記組成からルペロールを除いた組
成にて配合することにより製造した。Cream A is prepared by dissolving component (A) by heating.
The mixture was kept at 0 ° C., and the component (B) excluding the fragrance was separately heated and dissolved at 80 ° C., and the component (A) was added thereto with stirring, cooled, fragrance was added, and the mixture was further cooled. Was. The control for Cream B was prepared by blending with a composition excluding luperol from the above composition.
【００２３】実施例３ 化粧用油 成分 配合量（重量％） α−ルペオールアセテート ０．１ ステアリン酸コレステリル １．０ オリーブ油 ２．０ スクワラン 残部 スクワランに他の成分を均一に溶解して化粧用油を得た。Example 3 Cosmetic Oil Ingredients Compounding amount (% by weight) α-lupeol acetate 0.1 Cholesteryl stearate 1.0 Olive oil 2.0 Squalane Remainder Other components are uniformly dissolved in squalane to make cosmetic oil I got
【００２４】[0024]
【発明の効果】本発明によれば、皮膚機能を亢進し、皮
膚の老化防止に優れた効果を発揮する皮膚化粧料を得る
ことができる。According to the present invention, it is possible to obtain a skin cosmetic which enhances skin functions and exhibits an excellent effect of preventing skin aging.
───────────────────────────────────────────────────── フロントページの続き (56)参考文献 特開 平５−25023（ＪＰ，Ａ) 特開 平４−356407（ＪＰ，Ａ) 特開 平４−187658（ＪＰ，Ａ) 特開 平１−290619（ＪＰ，Ａ) (58)調査した分野(Int.Cl.6，ＤＢ名) A61K 7/00,8/48 ──────────────────────────────────────────────────続 き Continuation of the front page (56) References JP-A-5-25023 (JP, A) JP-A-4-356407 (JP, A) JP-A-4-187658 (JP, A) JP-A-1- 290619 (JP, A) (58) Field surveyed (Int. Cl. 6 , DB name) A61K 7/00, 8/48
Claims (1)
ステル誘導体を含有することを特徴とする皮膚化粧料。1. A skin cosmetic comprising lupeol and / or an organic acid ester derivative thereof.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP274192A JP2912489B2 (en) | 1992-01-10 | 1992-01-10 | Skin cosmetics |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP274192A JP2912489B2 (en) | 1992-01-10 | 1992-01-10 | Skin cosmetics |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPH05186326A JPH05186326A (en) | 1993-07-27 |
| JP2912489B2 true JP2912489B2 (en) | 1999-06-28 |
Family
ID=11537775
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP274192A Expired - Fee Related JP2912489B2 (en) | 1992-01-10 | 1992-01-10 | Skin cosmetics |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JP2912489B2 (en) |
Families Citing this family (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP3699543B2 (en) * | 1996-11-13 | 2005-09-28 | 株式会社ノエビア | Antibacterial agent and antibacterial cosmetic comprising the same |
| US6124362A (en) * | 1998-07-17 | 2000-09-26 | The Procter & Gamble Company | Method for regulating hair growth |
| US6482857B1 (en) | 1998-07-17 | 2002-11-19 | The University Of Texas Southwestern Medical Center | Compositions which contain triterpenes for regulating hair growth |
| JP4504520B2 (en) * | 2000-06-26 | 2010-07-14 | 花王株式会社 | Melanin production promoter |
| US6951847B2 (en) * | 2000-09-29 | 2005-10-04 | Regents Of The University Of Minnesota | Methods of treating fungal infections using lupeol |
| EP1349553B1 (en) * | 2001-01-12 | 2006-09-06 | Bsp Pharma | Dihydro-triterpenes in the treatment of viral infections, cardiovascular disease, inflammation, hypersensitivity or pain |
| FR2822821B1 (en) * | 2001-04-03 | 2004-05-07 | Pharmascience Lab | LUPINE SEED HULL EXTRACT CONTAINING LUPEOL, ESPECIALLY EXTRACT RICH IN LUPEOL AND PROCESS FOR PREPARING THE SAME |
| TWI332401B (en) * | 2001-08-21 | 2010-11-01 | Shiseido Co Ltd | Materials which are capable of enhancing laminin-5 producing ability in epidermal cell and the use thereof |
| WO2005039610A1 (en) * | 2003-10-24 | 2005-05-06 | Cognis France, S.A.S. | A plant extract and its pharmaceutical and cosmetic use |
| JP5291369B2 (en) * | 2008-03-31 | 2013-09-18 | 株式会社ナリス化粧品 | Keratinocyte growth promoter |
| CN104780927B (en) * | 2012-08-29 | 2019-06-11 | 花王株式会社 | Transglutaminase activation agent |
| JP6288759B2 (en) * | 2012-08-29 | 2018-03-07 | 花王株式会社 | Transglutaminase activator |
| JP6611308B2 (en) * | 2015-04-30 | 2019-11-27 | ポーラ化成工業株式会社 | Skin preparation |
-
1992
- 1992-01-10 JP JP274192A patent/JP2912489B2/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| JPH05186326A (en) | 1993-07-27 |
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