JP2592597Y2 - Chemical sensor plate and reference solution pack - Google Patents

Description

[Detailed description of the invention]

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a simple disposable chemical sensor plate for testing blood and the like, and a reference liquid pack used for the same.

[0002]

2. Description of the Related Art Several chemical sensors have been known as disposable simple chemical sensors for inspection. For example, as shown in FIG. 10, three pairs of a reference electrode 32 provided with an ion-sensitive film on a substrate 31 and a sample liquid measurement electrode 33 are provided, and these electrodes are insulated so as to form a window on the tip side. It is known that the sample solution is covered with a membrane 34, a reference solution and a sample solution are dropped on these windows, and respective electrodes are connected to a measurement circuit to simultaneously measure three ion concentrations of the sample solution.

[0003]

However, since the above type of structure is a flat plate structure, the amount of liquid that can be dropped is 10%.
It was limited to 50 μl. If the amount of the sample solution is dropped more than that, the sample solution and the reference solution are spilled or mixed, so that it is impossible to accurately measure the ion concentration of the sample solution.

To avoid this inconvenience, a dedicated pipette 35 shown in FIG.
It was necessary to aspirate a predetermined amount of the reference liquid on a and the sample liquid on the other end pipette 35b while checking the scale, and drip it over a small window. For this reason, the blood collected by a syringe to be inspected must be transferred to a dedicated test tube in which the above-mentioned dedicated pipette can be used. As described above, it is troublesome to use a dedicated pipette or a dedicated test tube, and it is very troublesome when an emergency is required.

Even if the above-mentioned dedicated pipette is used, a chemical sensor having a differential structure for obtaining a potential difference of a sample solution with respect to a reference solution, because of its principle and accuracy, simultaneously sucks up the reference solution and the sample solution to obtain the window portion. This is also troublesome, and cannot be used in an emergency.

In view of the above, according to the above-mentioned application for the registration of a utility model, dedicated flow paths communicating with the sample liquid measuring electrode and the reference electrode are respectively provided, and a sample liquid introducing section is provided in each dedicated flow path. Provision of a chemical sensor plate in which a sample liquid is injected into the sample liquid input section, the sealed state is released, and the sample liquid and the reference liquid are circulated so that they can be supplied to the sample liquid measurement electrode and the reference electrode, respectively. did.

However, in the case of the conventional method shown in FIG. 10, the reference liquid is marketed as a separate consumable in a bottle or the like. There is a problem that it may change, and the improved chemical sensor plate, which stores the reference solution, only seals the opening by bonding the sheet. The seal breaks,
The reference liquid may flow out into the flow passage.

An object of the present invention is to supply a sample liquid and a reference liquid to a predetermined electrode without using a dedicated pipette or the like so that the liquids do not mix with each other, and require a reference liquid having a certain concentration. An object of the present invention is to provide a chemical sensor plate which can be supplied by a simple operation at times and a reference liquid pack which can supply the reference liquid.

[0009]

According to the present invention, in order to solve the above-mentioned problems, a sample liquid measuring electrode coated with a chemically sensitive film to which a sample liquid is dropped and a reference electrode to which a reference liquid is dropped are one-to-one. At least one set is provided on the substrate as a set, and a closed flow path for supplying the sample liquid to the sample liquid measurement electrode and a closed flow path for supplying the reference liquid to the reference electrode are respectively dedicated on the substrate surface. And a sample liquid input section and a reference liquid input section communicating with the respective flow passages are provided.The sample liquid measurement electrode and the reference electrode are made conductive by ion transfer, and the reference liquid is stored in a sealed state and the reference liquid is supplied. An object of the present invention is to provide a chemical sensor plate provided with a reference liquid pack in which a breakable portion into which the reference liquid can be injected is formed in a liquid injection section.

According to the present invention, at least one pair of a sample liquid measuring electrode coated with a chemically sensitive film to which a sample liquid is dropped and a reference electrode to which a reference liquid is dropped is provided on a substrate. A sensor plate body, a lower outer cell having a concave portion for accommodating the sensor plate body, a closed flow passage for supplying a sample liquid to the sample liquid measurement electrode, and a closed flow path for supplying a reference liquid to the reference electrode. An upper exterior having flow passages formed on the substrate surface and having a sample liquid input section and a reference liquid input section communicating with the respective flow paths, wherein the sample liquid measurement electrode and the reference electrode are made conductive by ion transfer. A cell, a reference liquid pack containing the reference liquid in a sealed state and forming a breakable portion into which the reference liquid can be supplied in the reference liquid supply section, wherein the sensor plate body is provided with the upper outer cell and the lower outer cell. More and interior, there is provided a chemical sensor plate forming the external connection terminals exposed through the insertion hole formed in at least one of the upper exterior cells, lower exterior cells to the sample liquid measuring electrode and the reference electrode.

At this time, the reference liquid pack may be fixed to the reference liquid charging section or may be detachable.

Further, the present invention provides the above-mentioned reference liquid pack which is individualized and made into an independent part.

[0014]

[Function] Since the reference liquid pack contains the reference liquid in a sealed state and has a breakable portion, it can be stored without changing the concentration of the reference liquid when not in use, and can be broken by a simple operation when used. Thereby, the reference liquid can be supplied to a necessary portion. If this reference liquid pack is individualized and made into an independent part, it becomes more convenient to handle.

Next, an embodiment of the present invention will be described with reference to the drawings. Figure 1
As shown in FIG. 3, three sets of copper electrodes (not shown) having a predetermined shape are formed on the paper polyester substrate 1 in opposition, silver plating layers 2a and 2b are formed on each copper electrode, and silver plating layers are formed on the copper electrodes. A silver chloride layer (not shown) is formed on opposite ends of the layers 2a and 2b.

A window 4 is provided at the opposite end of the silver chloride layer.
a, 4b are formed, and an insulating layer 6 made of resin is formed so that the silver plating layers 2a, 2b at the other end portions are exposed to become external electrodes. An embankment body 8 having an elongated hole 7 corresponding to the portions 4a and 4b and portions including the portions therebetween is provided. Three different types of ion-sensitive film resin solutions are dropped into each of the elongated holes 7 and dried to form an ion-sensitive film on the silver chloride layer.

Further, spacers 10 having liquid supply holes 9a and 9b are provided on sensitive portions (corresponding to the windows 4a and 4b) of the ion sensitive membrane so as to overlap.

The sensor plate body thus completed is inserted into and accommodated in the recess 11a of the lower exterior cell 11 made of an acrylic or styrene resin, preferably a transparent resin. Further, an upper outer cell 12 is provided on the lower outer cell 11 to cover the sensor plate main body, and the sensor plate main body is internally provided by these cells.

The upper exterior cell 12 is provided with the liquid supply holes 9a,
9b, and further has a flat elongated box-shaped flow path extending to the substrate 1 side. The flow path is formed by a sample liquid flow path 13b and a reference liquid flow path 13c by a partition wall 13a separating the liquid supply holes 9a and 9b. A sample liquid input unit 14 and a reference liquid input unit 15 are provided on the base end side, and these input units are partitioned by extending and expanding the partition wall. 16
Reference numerals a and 16b denote three external terminal insertion holes provided in correspondence with the silver plating layers 2a and 2b of the electrodes.

As shown in FIG. 2, the sample liquid flow path 13
b, the bottom of the reference liquid flow passage 13c is entirely open, and the ion-sensitive membrane is exposed to these openings through the respective pairs of liquid supply holes 9a and 9b shown in FIG. The sample liquid input section 14,
The reference liquid inlet 15 is open at its top and bottom, and communicates with the flow passages 13b and 13c.

As shown in FIG. 3, the flow paths 13b, 1
On the upper surface of 3c, ventilation holes 13d, 13d,... Are provided so as to communicate with the sample liquid flow passage 13b and the reference liquid flow passage 13c, respectively, and the adhesive sheet 17 is removably adhered. In addition, a liquid junction hole 13e through which the liquid flows is provided at the tip of the partition wall 13a. The upper exterior cell 12 may be attached to the substrate 1 with a double-sided tape or an adhesive.

The upper opening of the sample liquid inlet 14 is sealed with a sealing sheet 18 such as an aluminum foil laminated with a plastic film, and a reference liquid pack 19 is fixed to the reference liquid inlet 15 with an adhesive. . As shown in FIG. 4, this reference liquid pack 19 is in the form of a separate PTP pack, and an aluminum foil 19 as a breakable portion is provided on a plate 19a.
b, covering the central cutout hole 19c, and attaching this plate to a deformable bulging lid 19d made of plastic or the like containing a reference liquid with the aluminum foil side inside.
Arc-shaped breaking projection 19 provided at the center of the bulging lid 19d
Pressing e breaks the aluminum foil so that the reference liquid can flow out.

At the time of use, the adhesive sheet 17 is peeled off and ventilated, and as shown by the imaginary line in FIG. 4, the breakage projection 19e of the reference liquid pack 19 is pushed out to discharge the reference liquid. The liquid passes through the passage 13c and sequentially fills the three sets of liquid supply holes 9b, and the reference liquid is supplied to the ion-sensitive membrane via the respective elongated holes 7. In this state, when blood is injected by piercing the needle of a syringe containing the collected blood into the sealing sheet 18 on the upper surface of the sample liquid input unit 14, the blood flows through the sample liquid flow passage 13b, and the three sets of blood are supplied. Liquid supply hole 9
a, and blood is supplied to the ion-sensitive membrane through the respective elongated holes 7. In this case, the partition wall 13
The gap of the elongated hole 7 of the embankment 8 via the spacer 10 below a is small, that is, if the thickness of the embankment 8 is reduced,
Since the ion-sensitive membrane repels water, crossing of the sample liquid and the reference liquid can be avoided, and the liquids can travel straight without mixing.

Thereafter, the three sets of silver plating layers 2a, 2b
The external terminals of the measurement circuit are
The three ion concentrations of blood can be measured simultaneously by inserting and inserting into a and 16b.

In the above description, the arc-shaped breaking projections are provided on the reference liquid pack 19. As shown in FIG. 5, instead of the breaking projections 19e shown in FIG. 4, separated rod-like projections 20a and 20b are replaced by elliptical plates 20c. The projection 20 for breaking attached to the body may be provided as a built-in separate body, in which case the outlet becomes smaller,
It is possible to continuously discharge the reference liquid little by little.

As shown in FIGS. 6 and 7, a reference liquid injection section 15 ′ is provided above the reference liquid injection section 15.
An outer cell 12 ′ similar to the above-mentioned outer cell 12 is provided except that the outer cell is formed in a cylindrical shape separated from the outer surface and a screw portion is formed on the outer surface thereof.
The reference liquid is put into a and the aluminum foil 21
The reference liquid pack 21 sealed with b may be provided, and the aluminum foil 21b may be broken by screwing the reference liquid pack 21 into the reference liquid injection section 15 ', so that the reference liquid flows out. In this manner, the reference liquid pack 21 can be securely mounted even in a place where the vibration is large, such as when the vehicle is transported on a bad road in a vehicle or when the vehicle is transported on a ship. 4 and 5 are provided on the reference liquid pack 21, and the reference liquid pack 21 is screwed into the reference liquid input portion 15 'so as not to leak the reference liquid, and then the protrusion is pressed. Thereby, the reference liquid may be caused to flow out.

As shown in FIG. 8, instead of the breaking projection 19e of FIG. 4, a breaking projection 22a having a conical arrowhead at the tip and a T-shaped rear end is provided. May be used, and in this case, the outflow amount can be controlled because the breaking diameter of the aluminum foil can be changed depending on the degree of penetration of the arrow.

As shown in FIG. 9, the cutout hole 23b at one end of the plate 23a is covered with an aluminum foil 23c, and the plate is provided with a flat body 23d at one end and at the other end. Affixed to a lid 23f having an easily deformable swelling body 23e containing a reference liquid, in which case the gap between the flat body 23d and the swelling body 23e is narrowed, and the space between the flattened body 23d and the swelling body 23e is joined with an adhesive having a weak adhesive force. Using the reference liquid pack 23 provided with the soft adhesive portion 23g, the soft adhesive portion 23g is broken by pushing the swelling container 23e, the reference liquid is sent to the flat container 23d, and the aluminum foil 23c is further broken by the liquid pressure. The reference liquid may be allowed to flow out. In this case, when the plate 23a is bent together with the swelling container 23e, the gap of the broken soft adhesive portion 23g is closed, so that the supply of the reference liquid can be stopped and the amount thereof can be adjusted. In addition, 2
Reference numeral 3h denotes an elastic fixing piece for fixing to the reference liquid input unit 15.

[0029]

According to the present invention, a reference liquid pack having a breakable part capable of storing a reference liquid in a sealed state in the reference liquid charging section of the chemical sensor plate and supplying the reference liquid to the charging section is formed. The standard solution can be stored for a long time without changing the reference solution concentration, and the reference solution can be supplied to the chemical sensor plate by simple operation. The reference liquid does not flow out even when the water is added, so that defective products outflowing from the reference liquid can be eliminated. These performances are further improved by individualizing the reference liquid pack into independent parts, thereby facilitating the handling. In this way, by using a chemical sensor plate that can measure the concentration of a sample liquid simply by supplying a reference liquid with a certain concentration to a dedicated flow path with a simple operation, and also supplying a sample liquid to the dedicated flow path. For example, the specific ion concentration of the collected blood can be measured quickly and accurately without the need for a dedicated pipette or test tube.

[Brief description of the drawings]

FIG. 1 is an exploded perspective view of a chemical sensor plate according to a first embodiment of the present invention.

FIG. 2 is a perspective view when the upper exterior cell of the embodiment is inverted.

FIG. 3 is a bottom view of the upper exterior cell.

FIG. 4 is a sectional view of the reference liquid pack according to the first embodiment of the present invention;

FIG. 5 is a sectional view of a reference liquid pack according to a second embodiment of the present invention.

FIG. 6 is a perspective view of a part of a chemical sensor plate using a reference liquid pack according to a third embodiment of the present invention;

FIG. 7 is a partial cross-sectional view of a reference liquid pack according to a third embodiment of the present invention in a used state;

FIG. 8 is a sectional view of a reference liquid pack according to a fourth embodiment of the present invention.

FIG. 9 is a partial cross-sectional view illustrating a use state of a reference liquid pack according to a fifth embodiment of the present invention.

FIG. 10 is a perspective view of a part of a conventional use case of a chemical sensor.

[Explanation of symbols]

 Reference Signs List 1 substrate 2a part of silver plating layer of sample liquid measurement electrode 2b part of silver plating layer of reference electrode 13b sample liquid flow passage 13c reference liquid flow passage 13e liquid junction hole 14 sample liquid input unit 15 reference liquid input unit 19; 21, 22, 23 Reference liquid pack

──────────────────────────────────────────────────続 き Continuation of the front page (56) References JP-A-62-39759 (JP, A) JP-A-62-93651 (JP, A) JP-A-62-29264 (JP, A) JP-A-58-1988 140635 (JP, A) Japanese Utility Model 62-152257 (JP, U) (58) Fields investigated (Int. Cl. ⁶ , DB name) G01N 27/28 331 G01N 27/28 G01N 27/416

Claims (6)

(57) [Scope of request for utility model registration] 1. A sample liquid measuring electrode coated with a chemically sensitive film to which a sample liquid is supplied and a reference electrode to which a reference liquid is supplied are provided in a one-to-one pair, and at least one pair is provided on a substrate. A closed flow path for supplying the sample liquid to the electrode and a closed flow path for supplying the reference liquid to the reference electrode are respectively provided on the substrate surface, and a sample liquid input section communicating with each flow path, A liquid injection section is provided, the sample liquid measurement electrode and the reference electrode are made conductive by ion transfer, and the reference liquid is accommodated in a sealed state to form a breakable portion into which the reference liquid can be injected. Chemical sensor plate with a reference liquid pack. 2. A sensor plate main body comprising: a sample liquid measuring electrode coated with a chemically sensitive film to which a sample liquid is supplied; and a pair of reference electrodes to which a reference liquid is supplied, and at least one set provided on a substrate. A lower exterior cell having a concave portion for accommodating the sensor plate body, a closed flow path for supplying a sample liquid to the sample liquid measurement electrode, and a closed flow path for supplying a reference liquid to the reference electrode. An upper exterior cell formed on the substrate surface and having a sample liquid input section and a reference liquid input section communicating with the respective flow passages, wherein the sample liquid measurement electrode and the reference electrode can be made conductive by ion transfer, and sealed. A reference liquid pack containing a reference liquid in a state and having a breakable portion capable of introducing the reference liquid into the reference liquid input section is provided, and the sensor plate body is internally provided by the upper outer cell and the lower outer cell. A chemical sensor plate in which an external connection terminal exposed through an insertion hole provided in at least one of the upper outer cell and the lower outer cell is formed in the sample liquid measurement electrode and the reference electrode. 3. The chemical sensor plate according to claim 1, wherein the reference liquid pack is fixed to the reference liquid inlet. 4. The chemical sensor plate according to claim 1, wherein the reference liquid pack is detachably provided in the reference liquid charging section. 5. The chemical sensor plate according to claim 3, wherein the breakable portion of the reference liquid pack is formed so as to be capable of opening and closing the break opening. 6. A reference liquid pack according to claim 1, wherein said reference liquid pack is individualized and made into an independent part.