JPH081491Y2 - Chemical sensors and sensor plates - Google Patents

Description

[Detailed description of the device]

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a disposable simple chemical sensor for testing blood and the like.

[0002]

2. Description of the Related Art Several chemical sensors have been known as disposable simple chemical sensors for inspection. For example, as shown in FIG. 10, three pairs of a reference electrode 22 and an analyte liquid measuring electrode 23 provided with an ion sensitive film on a substrate 21 are provided, and insulation is performed so that a window portion is formed on the tip side of these electrodes. It is known that the film is covered with a film 24, a reference solution and a sample solution are dropped on these windows, and respective electrodes are connected to a measurement circuit to simultaneously measure the three ion concentrations of the sample solution.

[0003]

However, since the structure of the above type is a flat plate structure, the amount of liquid that can be dropped is 10
It was limited to 50 μl. If the amount of the sample solution is dropped more than that, the sample solution and the reference solution are spilled or mixed, so that it is impossible to accurately measure the ion concentration of the sample solution.

In order to avoid this inconvenience, a dedicated pipette 25 shown in FIG.
It was necessary to suck a specified amount of the reference liquid in a and the sample liquid in the other tip pipette 25b while observing the scale, and drop the sample liquid through a small window. For this reason, the blood collected by a syringe to be inspected must be transferred to a dedicated test tube in which the above-mentioned dedicated pipette can be used. As described above, it is troublesome to use a dedicated pipette or a dedicated test tube, and it is very troublesome when an emergency is required.

Even if the above-mentioned dedicated pipette is used, a chemical sensor having a differential structure for obtaining a potential difference of a sample solution with respect to a reference solution, because of its principle and accuracy, simultaneously sucks up the reference solution and the sample solution to obtain the window portion. This is also troublesome, and cannot be used in an emergency.

An object of the present invention is to provide a chemical sensor and a sensor plate for supplying a sample liquid and a reference liquid to a predetermined electrode without causing the liquids to be mixed with each other. Another object of the present invention is to provide a chemical sensor and a sensor plate which are preliminarily provided with a reference liquid and are capable of simultaneously supplying the reference liquid when the specimen liquid is supplied.

[0007]

In order to solve the above-mentioned problems, the present invention provides at least one pair of a sample liquid measuring electrode coated with a chemosensitive film and a reference electrode to provide a specific sample liquid of the sample liquid. In a chemical sensor capable of measuring the concentration, a flow passage for supplying a sample liquid to the sample liquid measuring electrode and a flow passage for supplying a reference liquid to the reference electrode are respectively provided exclusively for communicating with the respective flow passages. The present invention provides a chemical sensor in which a sample liquid introduction part and a reference liquid introduction part are provided, and the sample liquid measurement electrode and the reference electrode can be electrically conductive by ion movement at the tip of each of the above flow paths .

Further, the sample solution measurement electrodes coated with chemical sensitive film, at least one set by the reference electrode pair provided on the substrate, and that flow passage to supply liquid specimen to the specimen liquid measuring electrode, the reference electrodes based solution that flow passage to supply respectively provided exclusively on the substrate surface, the sample liquid supplying portion for communicating the reference liquid supplying portion provided in each of the flow passage, at least the
The flow passage for the reference liquid is opened so that air is vented from the tip to the outside.
Mouth freely vent provided in, and the above-described sample liquid measuring electrode and the reference electrode there is provided a sensor plate which enables conducting an ion moving in the distal end of the respective flow passages.

At this time, the reference liquid inlet is a flow passage for the reference liquid.
The ventilation hole that vents to the outside from the tip provided on the
In this state, add the reference solution and seal it.
A reference electrode is formed by opening a hole to allow the reference liquid to flow through the flow passage.
Is preferably supplied to

[0010]

[Function] Since the sample liquid and the reference liquid are supplied to the sample liquid sensitive electrode and the reference electrode from the inlet through the dedicated flow passages, respectively, the liquid spills from each electrode to the other electrode. There is no. Also, for the reference liquid, if the flow passage is sealed in advance and stored in the charging section,
By opening and ventilating, it can be circulated through the flow passage and supplied to the reference electrode.

[0011]

Embodiments of the present invention will now be described with reference to the drawings. First, as shown in FIGS. 1 to 3, three pairs of copper electrodes 1a and 1b (not shown) having a predetermined shape are formed on a paper polyester substrate 1 so as to face each other, and a silver plating layer is formed on each copper electrode 1a and 1b. 2a, 2b are formed, and silver chloride layers 3a, 3b are formed on the opposite end sides of the respective silver plating layers 2a, 2b.
(Not shown) is formed.

Windows 4a and 4b are formed on the opposite end sides of the silver chloride layers 3a and 3b, and the silver plating layers 2a and 2b at the other ends of the silver chloride layers 3a and 3b are exposed so that they serve as external electrodes. Is formed on the insulating layer 6, and the spacer 8 having the elongated holes 7 corresponding to the windows 4a and 4b and the portion including the windows 4a and 4b is formed. Three different resin solutions for ion-sensitive films are dropped from the elongated holes 7 into the three sets of windows 4a and 4b, respectively, and dried to form ion-sensitive films 5a and 5b on the silver chloride layer. To be done.

In this way, the sensor plate is completed. Further, a liquid supply cell 9 made of an acrylic or styrene resin, preferably a transparent resin, is provided. This liquid supply cell covers the respective window portions 4a and 4b of each set through the elongated holes 7 and further has a flat elongated box-like flow passage extending to the substrate 1 side, and this flow passage has the window portion. A partition wall 10a that divides the 4a side and the 4b side divides the sample liquid flow passage 10b and the reference liquid flow passage 10c.
A sample liquid storage portion 11 and a reference liquid storage portion 12 as an input portion are respectively provided at the tips on the substrate side, and these storage portions are partitioned by extending and enlarging the partition wall.

As shown in FIG. 4, the sample liquid flow passage 10 is provided.
The bottom of b is opened over the entire surface, and the ion sensitive film 5a is exposed in this opening through the windows 4a of each set. On the other hand, the bottom portion of the reference liquid flow passage 10c is almost entirely opened at a portion covering the windows 4b of each set, the ion sensitive film 5b is exposed in this opening, and an elongated groove 10d communicating with the reference liquid storage portion is further provided. Has been formed. The sample liquid storage portion 11 and the reference liquid storage portion 12 are opened at their bottoms entirely, and the flow passages 10 are provided.
Connect to b and 10c.

Ventilation holes 10e, 10e, ... Are provided on the upper surfaces of the flow passages 10b, 10c so as to communicate with the sample liquid flow passage 10b and the reference liquid flow passage 10c, respectively, so that the adhesive sheet 13 can be peeled off. It is attached. A liquid junction hole 10f through which the liquid flows is provided at the tip of the partition wall 10a. The liquid supply cell 9 may be attached to the substrate 1 with a double-sided tape or an adhesive.

In such a structure, as shown in FIGS. 2 and 3, when not in use, the reference liquid reservoir 11 is filled with the reference liquid, while the sample liquid reservoir 12 is emptied to store these reservoirs. When the upper portions of the parts 11 and 12 are sealed with the aluminum foil 14, the ventilation hole 10e is sealed, the upper surface of the sample liquid storage part 11 is also sealed, and the reference liquid in the reference liquid storage part 12 does not flow out. At this time, the flow passage is formed in a capillary shape, and the elongated groove 10
Since the flow passage is narrowed by d, the outflow is effectively prevented.

When the adhesive sheet 13 is peeled off during use and the air is ventilated, the reference liquid passes through the elongated groove 10d and the reference liquid flow passage 10c.
And sequentially fills the three sets of windows 4b. When the needle of the syringe containing the blood collected in this state is pierced into the aluminum foil 14 on the upper surface of the sample liquid reservoir 11 to inject the blood, the blood circulates in the sample liquid flow passage 10b and each of the three sets of windows The portion 4a is sequentially filled. At this time, if the space of the elongated hole 7 under the partition wall 10a is made small, that is, the thickness of the spacer 8 is made thin, the insulating layer 6 repels water, so that crossing of the sample liquid and the reference liquid can be avoided, Both liquids can go straight without being mixed.

After that, by connecting a measuring circuit to the silver-plated layers 2a and 2b, it is possible to simultaneously measure the ion concentrations of three kinds of blood.

Although the elongated groove 10d is a straight groove in the above,
As shown in FIG. 5, the meandering groove 10g may be used, and in this case, the outflow of the reference liquid when not in use is better prevented.

Further, as shown in FIG. 6, silver plating layers 2a and 2b are provided.
Alternatively, the connector 15 may be formed on the end of the substrate 1 by wiring from the end without exposing.

Further, as shown in FIG. 7, a filter paper or other ion-permeable material 16 made of an ion-permeable material may be provided so as to project into both the reference liquid flow passage and the sample liquid flow passage through the partition wall 10a. . The adhesive sheet is omitted in FIGS.

Further, as shown in FIG. 8, the reference liquid flow passage 1
A protrusion 17 that is opened by bending at the tip of 0c may be provided.

Further, as shown in FIG. 9, the reference liquid flow passage 1
A thin portion 18 may be provided at the tip of 0c, which is ruptured by lightly pressing.

[0024]

EFFECTS OF THE INVENTION According to the present invention, a dedicated flow passage is provided for each of the sample liquid measuring electrode and the reference electrode, and the sample liquid and the reference liquid are supplied through these flow passages, respectively. It is possible to prevent a predetermined sample liquid and a reference liquid from being supplied to the respective electrodes so as to prevent them from overflowing from the electrodes and mixing with each other.

At this time, the sample liquid measurement electrode and the reference electrode are
At the tip of each of the above flow paths, ion migration
Since it is electrically conductive, the flow of the reference liquid and the sample liquid can be
It can be contacted at the tip, and both are mixed.
Effectively avoiding feeding to each electrode
Therefore, the accuracy of the test of the sample liquid can be improved. Well
At least from the tip of the reference liquid
Close the vent leading to the outside, sealed, its the sealing
If so saw reference liquid when solved by opening the vent flows, Accumulate advance reference liquid to the insertion portion, it is possible to distribute them in use, the sealed calibration work You can finish just by solving.

In this way, by providing a dedicated flow passage, the sample liquid,
When the reference liquids are supplied, the liquids can be supplied, for example, from a syringe that has collected blood, without the need for a dedicated pipette or test tube, and the work is extremely simple.

[Brief description of drawings]

FIG. 1 is an exploded perspective view of a sensor plate according to an embodiment of the present invention.

FIG. 2 is a completed perspective view of the sensor plate of the above embodiment.

FIG. 3 is a sectional view taken along line III-III in FIG. 2 of the sensor plate according to the embodiment.

FIG. 4 is a bottom perspective view of a liquid supply cell of the sensor plate of the above embodiment.

FIG. 5 is a top view of a sensor plate according to a second embodiment of the present invention.

FIG. 6 is a top view of a sensor plate according to a third embodiment of the present invention.

FIG. 7 is a top view of a sensor plate according to a fourth embodiment of the present invention.

FIG. 8 is a top view of a sensor plate according to a fifth embodiment of the present invention.

FIG. 9 is a top view of a sensor plate according to a sixth embodiment of the present invention.

FIG. 10 is a partial perspective view showing a state where a conventional chemical sensor is used.

[Explanation of reference numerals] 1 substrate 5a sample liquid measurement electrode 5b reference electrode 10b sample liquid flow passage 10c reference liquid flow passage 10e vent hole 10f liquid junction hole 11 sample liquid storage portion 12 reference liquid storage portion 13 adhesive sheet

Claims (4)

[Scope of utility model registration request] 1. A chemical sensor in which at least one pair of a sample liquid measuring electrode coated with a chemically sensitive film and a reference electrode are provided to measure the concentration of a specific substance in the sample liquid. A flow passage for supplying the sample liquid to the above and a flow passage for supplying the reference liquid to the above-mentioned reference electrode are provided respectively, and a sample liquid introducing section and a reference solution introducing section communicating with the respective flow paths are provided, and the sample solution is measured. A chemical sensor in which an electrode and a reference electrode are made conductive by ion movement at the tip of each of the above flow passages . 2. A flow passage for at least the reference liquid has a tip portion thereof.
From having an opening closable vent hole for venting to the outside, collected in the insertion portion of the reference fluid in its closed state, the vent pores during use
Opening to the chemical sensor according to claim 1, wherein supplying a reference liquid of the insertion portion into the base <br/> quasi electrodes were then circulated to the flow passage. 3. A chemical sensitive membrane coated specimen liquid measuring electrode, at least one set by the reference electrode pair disposed on a substrate, a flow passage you supplying sample fluid to the sample fluid measurement electrodes,
Provided exclusively the reference electrode as a reference solution that flow passage to supply on each said substrate surface, the sample liquid introduction part communicating with each of the flow passage, the reference liquid feeding section provided, at least the reference
The liquid flow passage has an opening for venting air from the tip to the outside.
A sensor plate in which existing ventilation holes are provided , and the sample liquid measurement electrode and the reference electrode can be electrically conductive by ion movement at the tips of the respective flow passages . 4. The reference liquid charging section is provided in the flow passage of the reference liquid.
A state in which the ventilation hole for ventilating from the tip to the outside is sealed
Then, the standard solution is charged and sealed, and the ventilation hole is opened at the time of use.
Then, the reference liquid is circulated in the flow passage and supplied to the reference electrode.
According to claim 3 Symbol round of the sensor plate that.