JP2562127B2 - 豚レラキシンをコード化する遺伝子配列の分子クローニングおよび特性化 - Google Patents
豚レラキシンをコード化する遺伝子配列の分子クローニングおよび特性化Info
- Publication number
- JP2562127B2 JP2562127B2 JP58021967A JP2196783A JP2562127B2 JP 2562127 B2 JP2562127 B2 JP 2562127B2 JP 58021967 A JP58021967 A JP 58021967A JP 2196783 A JP2196783 A JP 2196783A JP 2562127 B2 JP2562127 B2 JP 2562127B2
- Authority
- JP
- Japan
- Prior art keywords
- sequence
- mrna
- relaxin
- codons
- following
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
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- 229940088598 enzyme Drugs 0.000 description 1
- 238000012869 ethanol precipitation Methods 0.000 description 1
- 210000002219 extraembryonic membrane Anatomy 0.000 description 1
- 230000004927 fusion Effects 0.000 description 1
- 108020001507 fusion proteins Proteins 0.000 description 1
- 102000037865 fusion proteins Human genes 0.000 description 1
- 102000018146 globin Human genes 0.000 description 1
- 108060003196 globin Proteins 0.000 description 1
- ZJYYHGLJYGJLLN-UHFFFAOYSA-N guanidinium thiocyanate Chemical compound SC#N.NC(N)=N ZJYYHGLJYGJLLN-UHFFFAOYSA-N 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 229920001519 homopolymer Polymers 0.000 description 1
- 238000001114 immunoprecipitation Methods 0.000 description 1
- PLVPPLCLBIEYEA-UHFFFAOYSA-N indoleacrylic acid Natural products C1=CC=C2C(C=CC(=O)O)=CNC2=C1 PLVPPLCLBIEYEA-UHFFFAOYSA-N 0.000 description 1
- 230000001939 inductive effect Effects 0.000 description 1
- 238000002372 labelling Methods 0.000 description 1
- 210000003041 ligament Anatomy 0.000 description 1
- 244000144972 livestock Species 0.000 description 1
- 230000002101 lytic effect Effects 0.000 description 1
- 229910052943 magnesium sulfate Inorganic materials 0.000 description 1
- 235000019341 magnesium sulphate Nutrition 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 230000035772 mutation Effects 0.000 description 1
- -1 nucleotide triphosphates Chemical class 0.000 description 1
- 229940046166 oligodeoxynucleotide Drugs 0.000 description 1
- 108010091748 peptide A Proteins 0.000 description 1
- 239000000813 peptide hormone Substances 0.000 description 1
- 239000010452 phosphate Substances 0.000 description 1
- 239000013600 plasmid vector Substances 0.000 description 1
- 230000003389 potentiating effect Effects 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 108010066381 preproinsulin Proteins 0.000 description 1
- 239000002987 primer (paints) Substances 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 150000003212 purines Chemical class 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 230000037195 reproductive physiology Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 102220201851 rs143406017 Human genes 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 230000002194 synthesizing effect Effects 0.000 description 1
- 229960002180 tetracycline Drugs 0.000 description 1
- 229930101283 tetracycline Natural products 0.000 description 1
- 235000019364 tetracycline Nutrition 0.000 description 1
- 150000003522 tetracyclines Chemical class 0.000 description 1
- 229940035893 uracil Drugs 0.000 description 1
- 239000004246 zinc acetate Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/64—Relaxins
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Toxicology (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Gastroenterology & Hepatology (AREA)
- Endocrinology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Saccharide Compounds (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| AU2695/82 | 1982-02-12 | ||
| AUPF269582 | 1982-02-12 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS58201797A JPS58201797A (ja) | 1983-11-24 |
| JP2562127B2 true JP2562127B2 (ja) | 1996-12-11 |
Family
ID=3769360
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP58021967A Expired - Lifetime JP2562127B2 (ja) | 1982-02-12 | 1983-02-12 | 豚レラキシンをコード化する遺伝子配列の分子クローニングおよび特性化 |
Country Status (10)
| Country | Link |
|---|---|
| EP (1) | EP0086649B1 (en:Method) |
| JP (1) | JP2562127B2 (en:Method) |
| AT (1) | ATE45980T1 (en:Method) |
| CA (1) | CA1214738A (en:Method) |
| DE (1) | DE3380495D1 (en:Method) |
| DK (1) | DK61583A (en:Method) |
| ES (1) | ES519733A0 (en:Method) |
| GR (1) | GR78104B (en:Method) |
| IE (1) | IE55479B1 (en:Method) |
| NZ (1) | NZ203262A (en:Method) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ES8603579A1 (es) * | 1982-08-12 | 1985-12-16 | Florey Howard Inst | Un procedimiento para producir un vector de transferencia de adn. |
| CA1341012C (en) * | 1982-09-27 | 2000-06-06 | Biogen, Inc. | Dna sequences, recombinant dna molecules and processes for producing swine growth hormone-like polypeptides |
| IL70414A (en) * | 1982-12-13 | 1991-06-10 | Florey Howard Inst | Polypeptide having human h2-relaxin activity,double-stranded dna fragments coding therefor,vectors containing the dna fragments and methods for preparing the polypeptide,dna fragments and vectors |
| US5089419A (en) * | 1989-08-07 | 1992-02-18 | International Canine Genetics | Detection of pregnancy by identification of the c peptide of relaxin in the urine of animals |
| DK0640136T3 (da) * | 1992-03-27 | 2003-09-01 | Int Flower Dev Pty Ltd | Gensekvenser der koder for enzymer i flavonoidstofskiftevejen med en flavonoid-3'-hydroxylaseaktivitet samt deres anvendelse |
| CN106811470A (zh) * | 2015-11-30 | 2017-06-09 | 四川农业大学 | 一种犬松弛素原重组蛋白及犬松弛素原多克隆抗体的制备方法 |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ZA811895B (en) * | 1980-04-07 | 1982-04-28 | Univ California | Expression of hormone genomic clones |
| EP0068375A3 (en) * | 1981-06-22 | 1983-04-13 | G.D. Searle & Co. | Recombinant dna techniques for the production of relaxin |
-
1983
- 1983-02-02 IE IE197/83A patent/IE55479B1/en not_active IP Right Cessation
- 1983-02-09 GR GR70453A patent/GR78104B/el unknown
- 1983-02-11 EP EP83300714A patent/EP0086649B1/en not_active Expired
- 1983-02-11 ES ES519733A patent/ES519733A0/es active Granted
- 1983-02-11 AT AT83300714T patent/ATE45980T1/de active
- 1983-02-11 CA CA000421486A patent/CA1214738A/en not_active Expired
- 1983-02-11 DE DE8383300714T patent/DE3380495D1/de not_active Expired
- 1983-02-11 NZ NZ203262A patent/NZ203262A/en unknown
- 1983-02-11 DK DK61583A patent/DK61583A/da not_active Application Discontinuation
- 1983-02-12 JP JP58021967A patent/JP2562127B2/ja not_active Expired - Lifetime
Non-Patent Citations (3)
| Title |
|---|
| Ｂｉｏｃｈｅｍ，Ｂｉｏｐｈｙｓ，Ｒｅｓ，Ｃｏｍｍｕｎ．，［７５］Ｐ．５０３−５１０（１９７７） |
| Ｊ．Ｂｉｏｌ．Ｃｈｅｍ．，［２５５］Ｐ．７５２１−７５２４（１９８０） |
| Ｎａｔｕｒｅ，［２９１］Ｐ．１２７−１３１（１９８１） |
Also Published As
| Publication number | Publication date |
|---|---|
| EP0086649B1 (en) | 1989-08-30 |
| ES8503373A1 (es) | 1985-02-16 |
| ATE45980T1 (de) | 1989-09-15 |
| CA1214738A (en) | 1986-12-02 |
| IE830197L (en) | 1983-08-12 |
| JPS58201797A (ja) | 1983-11-24 |
| DK61583D0 (da) | 1983-02-11 |
| ES519733A0 (es) | 1985-02-16 |
| DE3380495D1 (en) | 1989-10-05 |
| IE55479B1 (en) | 1990-09-26 |
| NZ203262A (en) | 1986-07-11 |
| DK61583A (da) | 1983-08-13 |
| GR78104B (en:Method) | 1984-09-26 |
| EP0086649A3 (en) | 1984-03-28 |
| EP0086649A2 (en) | 1983-08-24 |
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