IE55479B1 - Molecular cloning and characterization of the gene sequence coding for porcine relaxin - Google Patents
Molecular cloning and characterization of the gene sequence coding for porcine relaxinInfo
- Publication number
- IE55479B1 IE55479B1 IE197/83A IE19783A IE55479B1 IE 55479 B1 IE55479 B1 IE 55479B1 IE 197/83 A IE197/83 A IE 197/83A IE 19783 A IE19783 A IE 19783A IE 55479 B1 IE55479 B1 IE 55479B1
- Authority
- IE
- Ireland
- Prior art keywords
- leu
- glu
- ser
- lys
- arg
- Prior art date
Links
- 108090000623 proteins and genes Proteins 0.000 title claims abstract description 24
- 108090000103 Relaxin Proteins 0.000 title claims description 46
- 102000003743 Relaxin Human genes 0.000 title claims description 45
- 238000012512 characterization method Methods 0.000 title description 3
- 238000010369 molecular cloning Methods 0.000 title description 3
- 108020004414 DNA Proteins 0.000 claims abstract description 47
- 108010090955 preprorelaxin Proteins 0.000 claims abstract description 36
- 238000000034 method Methods 0.000 claims abstract description 33
- 239000013598 vector Substances 0.000 claims abstract description 22
- 238000012546 transfer Methods 0.000 claims abstract description 19
- 108010087851 prorelaxin Proteins 0.000 claims abstract description 15
- 108010075254 C-Peptide Proteins 0.000 claims abstract description 10
- 108090000765 processed proteins & peptides Proteins 0.000 claims abstract description 9
- 108020004511 Recombinant DNA Proteins 0.000 claims abstract description 6
- 102000004196 processed proteins & peptides Human genes 0.000 claims abstract 2
- 239000002299 complementary DNA Substances 0.000 claims description 49
- 239000012634 fragment Substances 0.000 claims description 29
- 150000001413 amino acids Chemical class 0.000 claims description 24
- 108020004999 messenger RNA Proteins 0.000 claims description 23
- 239000013612 plasmid Substances 0.000 claims description 15
- 108020004705 Codon Proteins 0.000 claims description 9
- 210000004027 cell Anatomy 0.000 claims description 9
- 238000004519 manufacturing process Methods 0.000 claims description 9
- 102000053602 DNA Human genes 0.000 claims description 8
- 108010076504 Protein Sorting Signals Proteins 0.000 claims description 8
- 239000000523 sample Substances 0.000 claims description 8
- 102000004169 proteins and genes Human genes 0.000 claims description 7
- 108091008146 restriction endonucleases Proteins 0.000 claims description 7
- 210000004246 corpus luteum Anatomy 0.000 claims description 6
- 108020001507 fusion proteins Proteins 0.000 claims description 6
- 102000037865 fusion proteins Human genes 0.000 claims description 6
- 230000001580 bacterial effect Effects 0.000 claims description 5
- 230000000295 complement effect Effects 0.000 claims description 5
- 108010057821 leucylproline Proteins 0.000 claims description 5
- 108091033380 Coding strand Proteins 0.000 claims description 4
- 230000003362 replicative effect Effects 0.000 claims description 4
- VTJUNIYRYIAIHF-IUCAKERBSA-N Leu-Pro Chemical compound CC(C)C[C@H](N)C(=O)N1CCC[C@H]1C(O)=O VTJUNIYRYIAIHF-IUCAKERBSA-N 0.000 claims description 3
- 101500021084 Locusta migratoria 5 kDa peptide Proteins 0.000 claims description 3
- 108091034117 Oligonucleotide Proteins 0.000 claims description 2
- 239000006166 lysate Substances 0.000 claims description 2
- 238000012216 screening Methods 0.000 claims description 2
- 241001515965 unidentified phage Species 0.000 claims description 2
- OOCFXNOVSLSHAB-IUCAKERBSA-N Gly-Pro-Pro Chemical compound NCC(=O)N1CCC[C@H]1C(=O)N1[C@H](C(O)=O)CCC1 OOCFXNOVSLSHAB-IUCAKERBSA-N 0.000 claims 3
- IGUOAYLTQJLPPD-DCAQKATOSA-N Leu-Asn-Arg Chemical compound CC(C)C[C@H](N)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@H](C(O)=O)CCCN=C(N)N IGUOAYLTQJLPPD-DCAQKATOSA-N 0.000 claims 3
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- 244000005700 microbiome Species 0.000 claims 3
- 108010093296 prolyl-prolyl-alanine Proteins 0.000 claims 3
- 108010061238 threonyl-glycine Proteins 0.000 claims 3
- XYTNPQNAZREREP-XQXXSGGOSA-N Ala-Glu-Thr Chemical compound [H]N[C@@H](C)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H]([C@@H](C)O)C(O)=O XYTNPQNAZREREP-XQXXSGGOSA-N 0.000 claims 2
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- HJIFPJUEOGZWRI-GUBZILKMSA-N Glu-Asp-Lys Chemical compound C(CCN)C[C@@H](C(=O)O)NC(=O)[C@H](CC(=O)O)NC(=O)[C@H](CCC(=O)O)N HJIFPJUEOGZWRI-GUBZILKMSA-N 0.000 claims 2
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- COZMNNJEGNPDED-HOCLYGCPSA-N Gly-Val-Trp Chemical compound [H]NCC(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CC1=CNC2=C1C=CC=C2)C(O)=O COZMNNJEGNPDED-HOCLYGCPSA-N 0.000 claims 2
- RNKSNIBMTUYWSH-YFKPBYRVSA-N L-prolylglycine Chemical compound [O-]C(=O)CNC(=O)[C@@H]1CCC[NH2+]1 RNKSNIBMTUYWSH-YFKPBYRVSA-N 0.000 claims 2
- HYIFFZAQXPUEAU-QWRGUYRKSA-N Leu-Gly-Leu Chemical compound CC(C)C[C@H](N)C(=O)NCC(=O)N[C@H](C(O)=O)CC(C)C HYIFFZAQXPUEAU-QWRGUYRKSA-N 0.000 claims 2
- QNBVTHNJGCOVFA-AVGNSLFASA-N Leu-Leu-Glu Chemical compound CC(C)C[C@H](N)C(=O)N[C@@H](CC(C)C)C(=O)N[C@H](C(O)=O)CCC(O)=O QNBVTHNJGCOVFA-AVGNSLFASA-N 0.000 claims 2
- RXGLHDWAZQECBI-SRVKXCTJSA-N Leu-Leu-Ser Chemical compound CC(C)C[C@H](N)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CO)C(O)=O RXGLHDWAZQECBI-SRVKXCTJSA-N 0.000 claims 2
- WXUOJXIGOPMDJM-SRVKXCTJSA-N Leu-Lys-Asn Chemical compound CC(C)C[C@H](N)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC(N)=O)C(O)=O WXUOJXIGOPMDJM-SRVKXCTJSA-N 0.000 claims 2
- JIHDFWWRYHSAQB-GUBZILKMSA-N Leu-Ser-Glu Chemical compound CC(C)C[C@H](N)C(=O)N[C@@H](CO)C(=O)N[C@H](C(O)=O)CCC(O)=O JIHDFWWRYHSAQB-GUBZILKMSA-N 0.000 claims 2
- KNKHAVVBVXKOGX-JXUBOQSCSA-N Lys-Ala-Thr Chemical compound [H]N[C@@H](CCCCN)C(=O)N[C@@H](C)C(=O)N[C@@H]([C@@H](C)O)C(O)=O KNKHAVVBVXKOGX-JXUBOQSCSA-N 0.000 claims 2
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- KYYMILWEGJYPQZ-IHRRRGAJSA-N Phe-Glu-Glu Chemical compound OC(=O)CC[C@@H](C(O)=O)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@@H](N)CC1=CC=CC=C1 KYYMILWEGJYPQZ-IHRRRGAJSA-N 0.000 claims 2
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- 210000004899 c-terminal region Anatomy 0.000 claims 2
- 108010004073 cysteinylcysteine Proteins 0.000 claims 2
- 108010055341 glutamyl-glutamic acid Proteins 0.000 claims 2
- 108010018006 histidylserine Proteins 0.000 claims 2
- 108010029020 prolylglycine Proteins 0.000 claims 2
- 108010069117 seryl-lysyl-aspartic acid Proteins 0.000 claims 2
- IOFVWPYSRSCWHI-JXUBOQSCSA-N Ala-Thr-Leu Chemical compound CC(C)C[C@@H](C(O)=O)NC(=O)[C@H]([C@@H](C)O)NC(=O)[C@H](C)N IOFVWPYSRSCWHI-JXUBOQSCSA-N 0.000 claims 1
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/64—Relaxins
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Toxicology (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Gastroenterology & Hepatology (AREA)
- Endocrinology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Saccharide Compounds (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| AUPF269582 | 1982-02-12 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| IE830197L IE830197L (en) | 1983-08-12 |
| IE55479B1 true IE55479B1 (en) | 1990-09-26 |
Family
ID=3769360
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| IE197/83A IE55479B1 (en) | 1982-02-12 | 1983-02-02 | Molecular cloning and characterization of the gene sequence coding for porcine relaxin |
Country Status (10)
| Country | Link |
|---|---|
| EP (1) | EP0086649B1 (en:Method) |
| JP (1) | JP2562127B2 (en:Method) |
| AT (1) | ATE45980T1 (en:Method) |
| CA (1) | CA1214738A (en:Method) |
| DE (1) | DE3380495D1 (en:Method) |
| DK (1) | DK61583A (en:Method) |
| ES (1) | ES519733A0 (en:Method) |
| GR (1) | GR78104B (en:Method) |
| IE (1) | IE55479B1 (en:Method) |
| NZ (1) | NZ203262A (en:Method) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ES8603579A1 (es) * | 1982-08-12 | 1985-12-16 | Florey Howard Inst | Un procedimiento para producir un vector de transferencia de adn. |
| CA1341012C (en) * | 1982-09-27 | 2000-06-06 | Biogen, Inc. | Dna sequences, recombinant dna molecules and processes for producing swine growth hormone-like polypeptides |
| IL70414A (en) * | 1982-12-13 | 1991-06-10 | Florey Howard Inst | Polypeptide having human h2-relaxin activity,double-stranded dna fragments coding therefor,vectors containing the dna fragments and methods for preparing the polypeptide,dna fragments and vectors |
| US5089419A (en) * | 1989-08-07 | 1992-02-18 | International Canine Genetics | Detection of pregnancy by identification of the c peptide of relaxin in the urine of animals |
| DK0640136T3 (da) * | 1992-03-27 | 2003-09-01 | Int Flower Dev Pty Ltd | Gensekvenser der koder for enzymer i flavonoidstofskiftevejen med en flavonoid-3'-hydroxylaseaktivitet samt deres anvendelse |
| CN106811470A (zh) * | 2015-11-30 | 2017-06-09 | 四川农业大学 | 一种犬松弛素原重组蛋白及犬松弛素原多克隆抗体的制备方法 |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ZA811895B (en) * | 1980-04-07 | 1982-04-28 | Univ California | Expression of hormone genomic clones |
| EP0068375A3 (en) * | 1981-06-22 | 1983-04-13 | G.D. Searle & Co. | Recombinant dna techniques for the production of relaxin |
-
1983
- 1983-02-02 IE IE197/83A patent/IE55479B1/en not_active IP Right Cessation
- 1983-02-09 GR GR70453A patent/GR78104B/el unknown
- 1983-02-11 EP EP83300714A patent/EP0086649B1/en not_active Expired
- 1983-02-11 ES ES519733A patent/ES519733A0/es active Granted
- 1983-02-11 AT AT83300714T patent/ATE45980T1/de active
- 1983-02-11 CA CA000421486A patent/CA1214738A/en not_active Expired
- 1983-02-11 DE DE8383300714T patent/DE3380495D1/de not_active Expired
- 1983-02-11 NZ NZ203262A patent/NZ203262A/en unknown
- 1983-02-11 DK DK61583A patent/DK61583A/da not_active Application Discontinuation
- 1983-02-12 JP JP58021967A patent/JP2562127B2/ja not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| EP0086649B1 (en) | 1989-08-30 |
| ES8503373A1 (es) | 1985-02-16 |
| ATE45980T1 (de) | 1989-09-15 |
| CA1214738A (en) | 1986-12-02 |
| IE830197L (en) | 1983-08-12 |
| JPS58201797A (ja) | 1983-11-24 |
| DK61583D0 (da) | 1983-02-11 |
| ES519733A0 (es) | 1985-02-16 |
| DE3380495D1 (en) | 1989-10-05 |
| NZ203262A (en) | 1986-07-11 |
| DK61583A (da) | 1983-08-13 |
| JP2562127B2 (ja) | 1996-12-11 |
| GR78104B (en:Method) | 1984-09-26 |
| EP0086649A3 (en) | 1984-03-28 |
| EP0086649A2 (en) | 1983-08-24 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| MM4A | Patent lapsed |