JP2509271B2 - 遺伝子修飾され生体内で増殖された真核宿主細胞における誘導発現機構によるタンパク質の生産方法 - Google Patents

遺伝子修飾され生体内で増殖された真核宿主細胞における誘導発現機構によるタンパク質の生産方法

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Publication number
JP2509271B2
JP2509271B2 JP62506060A JP50606087A JP2509271B2 JP 2509271 B2 JP2509271 B2 JP 2509271B2 JP 62506060 A JP62506060 A JP 62506060A JP 50606087 A JP50606087 A JP 50606087A JP 2509271 B2 JP2509271 B2 JP 2509271B2
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JP
Japan
Prior art keywords
cells
gene
plasmid
medium
tumor
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
JP62506060A
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English (en)
Japanese (ja)
Other versions
JPH01501439A (ja
Inventor
ピーター ブロンリー
ミッシェル ドレアノ
ミッシュル フィッシュバッハ
ザビエル フイレ
プルダン パディウ
リチャード ベルミー
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
ROSUERU PUROPATEII Ltd
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ROSUERU PUROPATEII Ltd
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Application filed by ROSUERU PUROPATEII Ltd filed Critical ROSUERU PUROPATEII Ltd
Publication of JPH01501439A publication Critical patent/JPH01501439A/ja
Application granted granted Critical
Publication of JP2509271B2 publication Critical patent/JP2509271B2/ja
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/61—Growth hormone [GH], i.e. somatotropin
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027—New or modified breeds of vertebrates
    • A01K67/0271—Chimeric vertebrates, e.g. comprising exogenous cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/001—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination
    • C12N2830/002—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination inducible enhancer/promoter combination, e.g. hypoxia, iron, transcription factor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/80—Vector systems having a special element relevant for transcription from vertebrates
    • C12N2830/85—Vector systems having a special element relevant for transcription from vertebrates mammalian

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Environmental Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Endocrinology (AREA)
  • Biomedical Technology (AREA)
  • Plant Pathology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Toxicology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Animal Husbandry (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
JP62506060A 1986-10-15 1987-10-12 遺伝子修飾され生体内で増殖された真核宿主細胞における誘導発現機構によるタンパク質の生産方法 Expired - Lifetime JP2509271B2 (ja)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP86810455A EP0263908B1 (en) 1986-10-15 1986-10-15 Method for the production of proteins by means of inducible expression systems in genetically modified eukaryotic host-cells multiplicated in-vivo
AT86810455.5 1986-10-15

Publications (2)

Publication Number Publication Date
JPH01501439A JPH01501439A (ja) 1989-05-25
JP2509271B2 true JP2509271B2 (ja) 1996-06-19

Family

ID=8196470

Family Applications (1)

Application Number Title Priority Date Filing Date
JP62506060A Expired - Lifetime JP2509271B2 (ja) 1986-10-15 1987-10-12 遺伝子修飾され生体内で増殖された真核宿主細胞における誘導発現機構によるタンパク質の生産方法

Country Status (12)

Country Link
US (1) US5614381A (https=)
EP (2) EP0263908B1 (https=)
JP (1) JP2509271B2 (https=)
AT (1) ATE67787T1 (https=)
AU (1) AU612607B2 (https=)
CA (1) CA1319631C (https=)
DE (1) DE3681738D1 (https=)
DK (1) DK320888D0 (https=)
ES (1) ES2038602T3 (https=)
NO (1) NO882469D0 (https=)
WO (1) WO1988002778A1 (https=)
ZA (1) ZA877656B (https=)

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IL85728A (en) * 1987-03-25 1993-01-14 Bio Mega Inc Fluorine containing atrial natriuretic peptides, their preparation and pharmaceutical compositions containing them
AU612404B2 (en) * 1987-09-11 1991-07-11 Genentech Inc. Method for increasing the expression of polypeptides in recombinant cell culture
JPH03502882A (ja) * 1987-12-15 1991-07-04 ザ トラスティーズ オブ プリンストン ユニヴァーシティ 変異原物質および発癌物質に関するトランスジェニック試験システム
ZA892518B (en) * 1988-04-08 1989-12-27 Intracel Corp Method for the in-vivo production and testing of proteins by recombinant gene expression in selected host-cells
WO1990001543A1 (fr) * 1988-07-29 1990-02-22 Intracel Corporation Procede d'expression genetique de proteines heterologues par des cellules transfectees in vivo
GB8928884D0 (en) * 1989-12-21 1990-02-28 Feldmann Marc Transformed cell lines
GB9003791D0 (en) * 1990-02-20 1990-04-18 Ludwig Inst Cancer Res Transgenic animals,cell lines therefrom,and their use
US5866759A (en) * 1991-02-20 1999-02-02 Ludwig Institute For Cancer Research Transgenic mice expressing TSSV40 large T antigen
US5877398A (en) * 1993-01-29 1999-03-02 University Of British Columbia Biological systems incorporating stress-inducible genes and reporter constructs for environmental biomonitoring and toxicology
AU732872C (en) * 1996-08-15 2002-02-21 Government Of The United States Of America, As Represented By The Secretary Of The Department Of Health And Human Services, The Spatial and temporal control of gene expression using a heat shock protein promoter in combination with local heat
IT1294450B1 (it) * 1997-08-28 1999-03-25 Consiglio Nazionale Ricerche Animali transgenici per lo studio di agenti tossici chimici fisici o biologici
US7098191B2 (en) * 1997-11-03 2006-08-29 The Arizona Board Of Reagents Hyperthermic inducible expression vectors for gene therapy and methods of use thereof
US6709858B1 (en) * 1997-11-03 2004-03-23 The Arizona Board Of Regents On Behalf Of The University Of Arizona Hyperthermic inducible expression vectors for gene therapy and methods of use thereof
US7279565B2 (en) * 1998-05-05 2007-10-09 Richard Voellmy Molecular regulatory circuits to achieve sustained activation of genes of interest by a single stress
AUPQ233799A0 (en) * 1999-08-19 1999-09-09 Minister For Agriculture, Minister For Land And Water Conservation For And On Behalf Of The State Of New South Wales Recombinant sub-unit vaccine
US20030044398A1 (en) * 2001-03-20 2003-03-06 Robl James M. Methods for producing antibodies in mammals
CA2349506C (en) * 2001-06-14 2009-12-08 Duke University A method for selective expression of therapeutic genes by hyperthermia
US7935788B2 (en) * 2004-10-04 2011-05-03 National Research Council Of Canada Reverse cumate repressor mutant
CA2580515C (en) * 2004-10-04 2017-05-02 National Research Council Of Canada Expression system, components thereof and methods of use

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4399216A (en) * 1980-02-25 1983-08-16 The Trustees Of Columbia University Processes for inserting DNA into eucaryotic cells and for producing proteinaceous materials
JPS5729294A (en) * 1980-07-30 1982-02-17 Hayashibara Biochem Lab Inc Preparation of human insulin
JPS5852634B2 (ja) * 1980-12-05 1983-11-24 株式会社林原生物化学研究所 ウロキナ−ゼの製造法
IE56720B1 (en) * 1983-02-07 1991-11-20 Battelle Memorial Institute Methods and compositions for expression of competent eukaryotic gene products
AU7280487A (en) * 1986-04-04 1987-10-20 Battelle Memorial Institute Methods and compositions for expression of competent eukaryotic gene products
EP0262942A1 (en) * 1986-09-30 1988-04-06 Smithkline Beecham Corporation Cell transfection
EP0289034B1 (en) * 1987-05-01 1996-11-27 The General Hospital Corporation Transkaryotic implantation

Also Published As

Publication number Publication date
EP0263908A1 (en) 1988-04-20
JPH01501439A (ja) 1989-05-25
DK320888A (da) 1988-06-13
NO882469L (no) 1988-06-03
ES2038602T3 (es) 1993-08-01
AU8078487A (en) 1988-05-06
EP0285642A1 (en) 1988-10-12
DE3681738D1 (https=) 1991-10-31
DK320888D0 (da) 1988-06-13
WO1988002778A1 (en) 1988-04-21
ZA877656B (en) 1988-04-18
AU612607B2 (en) 1991-07-18
EP0263908B1 (en) 1991-09-25
US5614381A (en) 1997-03-25
ATE67787T1 (de) 1991-10-15
CA1319631C (en) 1993-06-29
NO882469D0 (no) 1988-06-03

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