JP2024533474A - 様々な内部修飾を有するループ型プライマーおよび標的検出のためのループドループ法 - Google Patents

様々な内部修飾を有するループ型プライマーおよび標的検出のためのループドループ法 Download PDF

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JP2024533474A
JP2024533474A JP2024515930A JP2024515930A JP2024533474A JP 2024533474 A JP2024533474 A JP 2024533474A JP 2024515930 A JP2024515930 A JP 2024515930A JP 2024515930 A JP2024515930 A JP 2024515930A JP 2024533474 A JP2024533474 A JP 2024533474A
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primer
oligonucleotide
loop
looped
clamping
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JP2024533474A5 (https=
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スコット ボール，キャメロン
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ユーエイチ－オーエイチ ラブス インコーポレイテッド
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
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    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6881—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for tissue or cell typing, e.g. human leukocyte antigen [HLA] probes
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    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/30—Oligonucleotides characterised by their secondary structure
    • C12Q2525/301—Hairpin oligonucleotides
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    • C12Q2527/00—Reactions demanding special reaction conditions
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    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/107—Nucleic acid detection characterized by the use of physical, structural and functional properties fluorescence
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    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/10—Detection mode being characterised by the assay principle
    • C12Q2565/101—Interaction between at least two labels
    • C12Q2565/1015—Interaction between at least two labels labels being on the same oligonucleotide
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    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/16—Primer sets for multiplex assays

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Virology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
JP2024515930A 2021-09-13 2022-09-10 様々な内部修飾を有するループ型プライマーおよび標的検出のためのループドループ法 Pending JP2024533474A (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US202163243625P 2021-09-13 2021-09-13
US63/243,625 2021-09-13
PCT/US2022/076247 WO2023039553A2 (en) 2021-09-13 2022-09-10 Looped primer with various internal modifications and loop-de-loop method for target detection

Publications (2)

Publication Number Publication Date
JP2024533474A true JP2024533474A (ja) 2024-09-12
JP2024533474A5 JP2024533474A5 (https=) 2025-09-18

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JP2024515930A Pending JP2024533474A (ja) 2021-09-13 2022-09-10 様々な内部修飾を有するループ型プライマーおよび標的検出のためのループドループ法

Country Status (8)

Country Link
US (1) US20240376528A1 (https=)
EP (1) EP4402285A4 (https=)
JP (1) JP2024533474A (https=)
KR (1) KR20240067091A (https=)
CN (1) CN118103526A (https=)
AU (1) AU2022343742A1 (https=)
CA (1) CA3232224A1 (https=)
WO (1) WO2023039553A2 (https=)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB202408733D0 (en) * 2024-06-18 2024-07-31 Univ Oxford Innovation Ltd Rapid nucleic acid amplification method

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004068112A2 (en) * 2003-01-28 2004-08-12 Gorilla Genomics, Inc. Hairpin primer amplification
US8815514B2 (en) * 2009-07-02 2014-08-26 Co-Diagnostics, Inc. Detection primers for nucleic acid extension or amplification reactions
WO2016025878A1 (en) * 2014-08-14 2016-02-18 Abbott Molecular Inc. Multifunctional oligonucleotides
ITUB20159332A1 (it) * 2015-12-22 2017-06-22 Diasorin S P A Procedimento di rivelazione in fluorescenza dell?amplificazione isotermica mediata da loop (LAMP) di un acido nucleico bersaglio, relativi oligonucleotidi e kit.
CA3118000A1 (en) * 2018-10-29 2020-05-07 Cepheid Exponential base-3 nucleic acid amplification with reduced amplification time using nested overlapping primers
US11377683B2 (en) * 2020-03-13 2022-07-05 Uh-Oh Labs Inc. Looped primer and loop-de-loop method for detecting target nucleic acid

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Publication number Publication date
CN118103526A (zh) 2024-05-28
WO2023039553A2 (en) 2023-03-16
CA3232224A1 (en) 2023-03-16
KR20240067091A (ko) 2024-05-16
EP4402285A4 (en) 2025-09-24
US20240376528A1 (en) 2024-11-14
WO2023039553A3 (en) 2023-07-20
EP4402285A2 (en) 2024-07-24
AU2022343742A1 (en) 2024-04-11

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