CN118103526A - 用于靶标检测的具有多种内部修饰的环状引物和loop-de-loop方法 - Google Patents
用于靶标检测的具有多种内部修饰的环状引物和loop-de-loop方法 Download PDFInfo
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- CN118103526A CN118103526A CN202280068910.6A CN202280068910A CN118103526A CN 118103526 A CN118103526 A CN 118103526A CN 202280068910 A CN202280068910 A CN 202280068910A CN 118103526 A CN118103526 A CN 118103526A
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
- C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
- C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
- C12Q1/701—Specific hybridization probes
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6881—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for tissue or cell typing, e.g. human leukocyte antigen [HLA] probes
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- C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
- C12Q2525/30—Oligonucleotides characterised by their secondary structure
- C12Q2525/301—Hairpin oligonucleotides
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- C12Q2527/00—Reactions demanding special reaction conditions
- C12Q2527/101—Temperature
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
- C12Q2563/107—Nucleic acid detection characterized by the use of physical, structural and functional properties fluorescence
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- C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
- C12Q2565/10—Detection mode being characterised by the assay principle
- C12Q2565/101—Interaction between at least two labels
- C12Q2565/1015—Interaction between at least two labels labels being on the same oligonucleotide
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- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/16—Primer sets for multiplex assays
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- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Engineering & Computer Science (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Virology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US202163243625P | 2021-09-13 | 2021-09-13 | |
| US63/243,625 | 2021-09-13 | ||
| PCT/US2022/076247 WO2023039553A2 (en) | 2021-09-13 | 2022-09-10 | Looped primer with various internal modifications and loop-de-loop method for target detection |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN118103526A true CN118103526A (zh) | 2024-05-28 |
Family
ID=85507042
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202280068910.6A Pending CN118103526A (zh) | 2021-09-13 | 2022-09-10 | 用于靶标检测的具有多种内部修饰的环状引物和loop-de-loop方法 |
Country Status (8)
| Country | Link |
|---|---|
| US (1) | US20240376528A1 (https=) |
| EP (1) | EP4402285A4 (https=) |
| JP (1) | JP2024533474A (https=) |
| KR (1) | KR20240067091A (https=) |
| CN (1) | CN118103526A (https=) |
| AU (1) | AU2022343742A1 (https=) |
| CA (1) | CA3232224A1 (https=) |
| WO (1) | WO2023039553A2 (https=) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB202408733D0 (en) * | 2024-06-18 | 2024-07-31 | Univ Oxford Innovation Ltd | Rapid nucleic acid amplification method |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2004068112A2 (en) * | 2003-01-28 | 2004-08-12 | Gorilla Genomics, Inc. | Hairpin primer amplification |
| US8815514B2 (en) * | 2009-07-02 | 2014-08-26 | Co-Diagnostics, Inc. | Detection primers for nucleic acid extension or amplification reactions |
| WO2016025878A1 (en) * | 2014-08-14 | 2016-02-18 | Abbott Molecular Inc. | Multifunctional oligonucleotides |
| ITUB20159332A1 (it) * | 2015-12-22 | 2017-06-22 | Diasorin S P A | Procedimento di rivelazione in fluorescenza dell?amplificazione isotermica mediata da loop (LAMP) di un acido nucleico bersaglio, relativi oligonucleotidi e kit. |
| CA3118000A1 (en) * | 2018-10-29 | 2020-05-07 | Cepheid | Exponential base-3 nucleic acid amplification with reduced amplification time using nested overlapping primers |
| US11377683B2 (en) * | 2020-03-13 | 2022-07-05 | Uh-Oh Labs Inc. | Looped primer and loop-de-loop method for detecting target nucleic acid |
-
2022
- 2022-09-10 US US18/691,809 patent/US20240376528A1/en active Pending
- 2022-09-10 AU AU2022343742A patent/AU2022343742A1/en active Pending
- 2022-09-10 JP JP2024515930A patent/JP2024533474A/ja active Pending
- 2022-09-10 CN CN202280068910.6A patent/CN118103526A/zh active Pending
- 2022-09-10 EP EP22868363.7A patent/EP4402285A4/en active Pending
- 2022-09-10 KR KR1020247012235A patent/KR20240067091A/ko active Pending
- 2022-09-10 CA CA3232224A patent/CA3232224A1/en active Pending
- 2022-09-10 WO PCT/US2022/076247 patent/WO2023039553A2/en not_active Ceased
Also Published As
| Publication number | Publication date |
|---|---|
| WO2023039553A2 (en) | 2023-03-16 |
| CA3232224A1 (en) | 2023-03-16 |
| KR20240067091A (ko) | 2024-05-16 |
| EP4402285A4 (en) | 2025-09-24 |
| US20240376528A1 (en) | 2024-11-14 |
| JP2024533474A (ja) | 2024-09-12 |
| WO2023039553A3 (en) | 2023-07-20 |
| EP4402285A2 (en) | 2024-07-24 |
| AU2022343742A1 (en) | 2024-04-11 |
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