JP2023548581A - Ｃｒｉｓｐｒ－ｃａｓ系を含むシュードモナスに対するファージ組成物およびその使用方法 - Google Patents

Ｃｒｉｓｐｒ－ｃａｓ系を含むシュードモナスに対するファージ組成物およびその使用方法 Download PDF

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JP2023548581A
JP2023548581A JP2023527227A JP2023527227A JP2023548581A JP 2023548581 A JP2023548581 A JP 2023548581A JP 2023527227 A JP2023527227 A JP 2023527227A JP 2023527227 A JP2023527227 A JP 2023527227A JP 2023548581 A JP2023548581 A JP 2023548581A
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bacteriophage
virus
polypeptide
crispr
cas system
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JP2023548581A5 (https=
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ガロフォロ，ポール
ジー． オースターアウト，デビッド
セル，カート
テュソン，ハンナ，ヒューイット
マクミラン，ラナ
マッキー，ロバート
ペンケ，テイラー
スティール，ショーン
ラドナー，クリストファー
アル－ジャリス，ガブリエル
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ローカス バイオサイエンシーズ，インク．
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/40—Viruses, e.g. bacteriophages
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2795/00—Bacteriophages
    • C12N2795/00011—Details
    • C12N2795/10011—Details dsDNA Bacteriophages
    • C12N2795/10041—Use of virus, viral particle or viral elements as a vector
    • C12N2795/10043—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Virology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Agronomy & Crop Science (AREA)
  • Pest Control & Pesticides (AREA)
  • Dentistry (AREA)
  • Environmental Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
JP2023527227A 2020-11-05 2021-11-04 Ｃｒｉｓｐｒ－ｃａｓ系を含むシュードモナスに対するファージ組成物およびその使用方法 Pending JP2023548581A (ja)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US202063110288P 2020-11-05 2020-11-05
US63/110,288 2020-11-05
US202163184728P 2021-05-05 2021-05-05
US63/184,728 2021-05-05
PCT/US2021/058123 WO2022098916A1 (en) 2020-11-05 2021-11-04 Phage compositions for pseudomonas comprising crispr-cas systems and methods of use thereof

Publications (2)

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JP2023548581A true JP2023548581A (ja) 2023-11-17
JP2023548581A5 JP2023548581A5 (https=) 2024-11-13

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US (1) US20240011041A1 (https=)
EP (1) EP4240165A4 (https=)
JP (1) JP2023548581A (https=)
AU (1) AU2021373795A1 (https=)
CA (1) CA3197191A1 (https=)
TW (1) TW202233832A (https=)
UY (1) UY39506A (https=)
WO (1) WO2022098916A1 (https=)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2025206239A1 (ja) * 2024-03-28 2025-10-02 国立感染症研究所長が代表する日本国 組換えファージ及び遺伝子のスクリーニング方法

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115960895B (zh) * 2022-08-09 2026-04-10 中国科学院微生物研究所 一种偶联的crispr和毒素-抗毒素元件及其在消杀耐药细菌中的应用
EP4712993A2 (en) * 2023-05-17 2026-03-25 The Regents of the University of California Phage therapy for alcohol-associated hepatitis
WO2025076308A1 (en) * 2023-10-06 2025-04-10 University Of Florida Research Foundation, Incorporated Compositions and methods for analyzing the effect of microbiota on host protein conformation and treating neurodegenerative diseases
CN117959655A (zh) * 2024-01-31 2024-05-03 四川大学 一种假单胞菌还原剂及其在钒还原中的应用和方法

Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2017536831A (ja) * 2014-11-26 2017-12-14 テクノロジー イノベーション モメンタム ファンド（イスラエル）リミテッド パートナーシップＴｅｃｈｎｏｌｏｇｙ Ｉｎｎｏｖａｔｉｏｎ Ｍｏｍｅｎｔｕｍ Ｆｕｎｄ（ｉｓｒａｅｌ）Ｌｉｍｉｔｅｄ Ｐａｒｔｎｅｒｓｈｉｐ 細菌遺伝子の標的化削減
JP2018518163A (ja) * 2015-06-15 2018-07-12 ノース カロライナ ステート ユニバーシティＮｏｒｔｈ Ｃａｒｏｌｉｎａ Ｓｔａｔｅ Ｕｎｉｖｅｒｓｉｔｙ 核酸およびｒｎａに基づく抗菌剤の効率的な送達のための方法および組成物
WO2019105821A1 (en) * 2017-11-29 2019-06-06 Snipr Biome Aps. Phage and transduction particles
WO2019213592A1 (en) * 2018-05-04 2019-11-07 Locus Biosciences, Inc. Methods and compositions for killing a target bacterium
WO2019227080A1 (en) * 2018-05-25 2019-11-28 Locus Biosciences, Inc. Methods and compositions for killing a target bacterium
JP2020525049A (ja) * 2017-06-25 2020-08-27 エスエヌアイピーアール・テクノロジーズ・リミテッドＳｎｉｐｒ Ｔｅｃｈｎｏｌｏｇｉｅｓ Ｌｉｍｉｔｅｄ 微生物集団の変更及び細菌叢の改変
JP2020528762A (ja) * 2017-06-25 2020-10-01 エスエヌアイピーアール・テクノロジーズ・リミテッドＳｎｉｐｒ Ｔｅｃｈｎｏｌｏｇｉｅｓ Ｌｉｍｉｔｅｄ ベクター及び方法
JP2023500336A (ja) * 2019-11-06 2023-01-05 ローカス バイオサイエンシーズ，インク． Ｃｒｉｓｐｒ－ｃａｓシステムを含むファージ組成物およびその使用方法

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9896696B2 (en) * 2016-02-15 2018-02-20 Benson Hill Biosystems, Inc. Compositions and methods for modifying genomes

Patent Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2017536831A (ja) * 2014-11-26 2017-12-14 テクノロジー イノベーション モメンタム ファンド（イスラエル）リミテッド パートナーシップＴｅｃｈｎｏｌｏｇｙ Ｉｎｎｏｖａｔｉｏｎ Ｍｏｍｅｎｔｕｍ Ｆｕｎｄ（ｉｓｒａｅｌ）Ｌｉｍｉｔｅｄ Ｐａｒｔｎｅｒｓｈｉｐ 細菌遺伝子の標的化削減
JP2018518163A (ja) * 2015-06-15 2018-07-12 ノース カロライナ ステート ユニバーシティＮｏｒｔｈ Ｃａｒｏｌｉｎａ Ｓｔａｔｅ Ｕｎｉｖｅｒｓｉｔｙ 核酸およびｒｎａに基づく抗菌剤の効率的な送達のための方法および組成物
JP2020525049A (ja) * 2017-06-25 2020-08-27 エスエヌアイピーアール・テクノロジーズ・リミテッドＳｎｉｐｒ Ｔｅｃｈｎｏｌｏｇｉｅｓ Ｌｉｍｉｔｅｄ 微生物集団の変更及び細菌叢の改変
JP2020528762A (ja) * 2017-06-25 2020-10-01 エスエヌアイピーアール・テクノロジーズ・リミテッドＳｎｉｐｒ Ｔｅｃｈｎｏｌｏｇｉｅｓ Ｌｉｍｉｔｅｄ ベクター及び方法
WO2019105821A1 (en) * 2017-11-29 2019-06-06 Snipr Biome Aps. Phage and transduction particles
WO2019213592A1 (en) * 2018-05-04 2019-11-07 Locus Biosciences, Inc. Methods and compositions for killing a target bacterium
WO2019227080A1 (en) * 2018-05-25 2019-11-28 Locus Biosciences, Inc. Methods and compositions for killing a target bacterium
JP2023500336A (ja) * 2019-11-06 2023-01-05 ローカス バイオサイエンシーズ，インク． Ｃｒｉｓｐｒ－ｃａｓシステムを含むファージ組成物およびその使用方法

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
CHEGINI, Z., ET AL.: ""Bacteriophage therapy against Pseudomonas aeruginosa biofilm: a review."", ANNALS OF CLINICAL MICROBIOLOGY AND ANTIMICROBIALS, vol. 19, JPN6025045102, 30 September 2020 (2020-09-30), pages 45 - 17, ISSN: 0005723003 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2025206239A1 (ja) * 2024-03-28 2025-10-02 国立感染症研究所長が代表する日本国 組換えファージ及び遺伝子のスクリーニング方法

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UY39506A (es) 2022-05-31
EP4240165A1 (en) 2023-09-13
TW202233832A (zh) 2022-09-01
CA3197191A1 (en) 2022-05-12
AU2021373795A9 (en) 2024-10-03
WO2022098916A1 (en) 2022-05-12
AU2021373795A1 (en) 2023-06-22
US20240011041A1 (en) 2024-01-11
EP4240165A4 (en) 2025-04-02

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