AU2021373795A1 - Phage compositions for pseudomonas comprising crispr-cas systems and methods of use thereof - Google Patents

Phage compositions for pseudomonas comprising crispr-cas systems and methods of use thereof Download PDF

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Publication number
AU2021373795A1
AU2021373795A1 AU2021373795A AU2021373795A AU2021373795A1 AU 2021373795 A1 AU2021373795 A1 AU 2021373795A1 AU 2021373795 A AU2021373795 A AU 2021373795A AU 2021373795 A AU2021373795 A AU 2021373795A AU 2021373795 A1 AU2021373795 A1 AU 2021373795A1
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AU
Australia
Prior art keywords
bacteriophage
polypeptide
crispr
sequence
cas system
Prior art date
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Abandoned
Application number
AU2021373795A
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English (en)
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AU2021373795A9 (en
Inventor
Gabriel AL-GHALITH
Paul GAROFOLO
Christopher LADNER
Robert Mckee
Lana MCMILLAN
David G. Ousterout
Taylor PENKE
Kurt SELLE
Shaun STEELE
Hannah Hewitt TUSON
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Locus Biosciences Inc
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Locus Biosciences Inc
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Filing date
Publication date
Application filed by Locus Biosciences Inc filed Critical Locus Biosciences Inc
Publication of AU2021373795A1 publication Critical patent/AU2021373795A1/en
Publication of AU2021373795A9 publication Critical patent/AU2021373795A9/en
Abandoned legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/40—Viruses, e.g. bacteriophages
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2795/00—Bacteriophages
    • C12N2795/00011—Details
    • C12N2795/10011—Details dsDNA Bacteriophages
    • C12N2795/10041—Use of virus, viral particle or viral elements as a vector
    • C12N2795/10043—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Virology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Agronomy & Crop Science (AREA)
  • Pest Control & Pesticides (AREA)
  • Dentistry (AREA)
  • Environmental Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
AU2021373795A 2020-11-05 2021-11-04 Phage compositions for pseudomonas comprising crispr-cas systems and methods of use thereof Abandoned AU2021373795A1 (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US202063110288P 2020-11-05 2020-11-05
US63/110,288 2020-11-05
US202163184728P 2021-05-05 2021-05-05
US63/184,728 2021-05-05
PCT/US2021/058123 WO2022098916A1 (en) 2020-11-05 2021-11-04 Phage compositions for pseudomonas comprising crispr-cas systems and methods of use thereof

Publications (2)

Publication Number Publication Date
AU2021373795A1 true AU2021373795A1 (en) 2023-06-22
AU2021373795A9 AU2021373795A9 (en) 2024-10-03

Family

ID=81458404

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2021373795A Abandoned AU2021373795A1 (en) 2020-11-05 2021-11-04 Phage compositions for pseudomonas comprising crispr-cas systems and methods of use thereof

Country Status (8)

Country Link
US (1) US20240011041A1 (https=)
EP (1) EP4240165A4 (https=)
JP (1) JP2023548581A (https=)
AU (1) AU2021373795A1 (https=)
CA (1) CA3197191A1 (https=)
TW (1) TW202233832A (https=)
UY (1) UY39506A (https=)
WO (1) WO2022098916A1 (https=)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115960895B (zh) * 2022-08-09 2026-04-10 中国科学院微生物研究所 一种偶联的crispr和毒素-抗毒素元件及其在消杀耐药细菌中的应用
EP4712993A2 (en) * 2023-05-17 2026-03-25 The Regents of the University of California Phage therapy for alcohol-associated hepatitis
WO2025076308A1 (en) * 2023-10-06 2025-04-10 University Of Florida Research Foundation, Incorporated Compositions and methods for analyzing the effect of microbiota on host protein conformation and treating neurodegenerative diseases
CN117959655A (zh) * 2024-01-31 2024-05-03 四川大学 一种假单胞菌还原剂及其在钒还原中的应用和方法
WO2025206239A1 (ja) * 2024-03-28 2025-10-02 国立感染症研究所長が代表する日本国 組換えファージ及び遺伝子のスクリーニング方法

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107208070B (zh) * 2014-11-26 2021-09-07 技术创新动力基金（以色列）有限合伙公司 细菌基因的靶向消除
WO2016205276A1 (en) * 2015-06-15 2016-12-22 North Carolina State University Methods and compositions for efficient delivery of nucleic acids and rna-based antimicrobials
US9896696B2 (en) * 2016-02-15 2018-02-20 Benson Hill Biosystems, Inc. Compositions and methods for modifying genomes
GB201710126D0 (en) * 2017-06-25 2017-08-09 Snipr Tech Ltd Vectors & Methods
JP2020525049A (ja) * 2017-06-25 2020-08-27 エスエヌアイピーアール・テクノロジーズ・リミテッドＳｎｉｐｒ Ｔｅｃｈｎｏｌｏｇｉｅｓ Ｌｉｍｉｔｅｄ 微生物集団の変更及び細菌叢の改変
US20190160120A1 (en) * 2017-11-29 2019-05-30 Snipr Biome Aps Dna, methods etc
CA3099316A1 (en) * 2018-05-04 2019-11-07 Locus Biosciences, Inc. Methods and compositions for killing a target bacterium
WO2019227080A1 (en) * 2018-05-25 2019-11-28 Locus Biosciences, Inc. Methods and compositions for killing a target bacterium
CN114981418A (zh) * 2019-11-06 2022-08-30 洛可斯生物科学公司 包含crispr-cas系统的噬菌体组合物及其使用方法

Also Published As

Publication number Publication date
JP2023548581A (ja) 2023-11-17
UY39506A (es) 2022-05-31
EP4240165A1 (en) 2023-09-13
TW202233832A (zh) 2022-09-01
CA3197191A1 (en) 2022-05-12
AU2021373795A9 (en) 2024-10-03
WO2022098916A1 (en) 2022-05-12
US20240011041A1 (en) 2024-01-11
EP4240165A4 (en) 2025-04-02

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MK1 Application lapsed section 142(2)(a) - no request for examination in relevant period