JP2023542705A - 核酸塩基編集のためのジンクフィンガー融合タンパク質 - Google Patents

核酸塩基編集のためのジンクフィンガー融合タンパク質 Download PDF

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Publication number
JP2023542705A
JP2023542705A JP2023519028A JP2023519028A JP2023542705A JP 2023542705 A JP2023542705 A JP 2023542705A JP 2023519028 A JP2023519028 A JP 2023519028A JP 2023519028 A JP2023519028 A JP 2023519028A JP 2023542705 A JP2023542705 A JP 2023542705A
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seq
domain
zfp
fusion protein
cytidine deaminase
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JP2023519028A
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Japanese (ja)
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JP2023542705A5 (https=
Inventor
エイ ファウザー，フリードリック
シー ミラー，ジェフリー
アラングンディ，セバスチャン
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Sangamo Therapeutics Inc
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Sangamo Therapeutics Inc
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Publication of JP2023542705A publication Critical patent/JP2023542705A/ja
Publication of JP2023542705A5 publication Critical patent/JP2023542705A5/ja
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y305/00—Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5)
    • C12Y305/04—Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5) in cyclic amidines (3.5.4)
    • C12Y305/04005—Cytidine deaminase (3.5.4.5)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/80—Fusion polypeptide containing a DNA binding domain, e.g. Lacl or Tet-repressor
    • C07K2319/81—Fusion polypeptide containing a DNA binding domain, e.g. Lacl or Tet-repressor containing a Zn-finger domain for DNA binding
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
JP2023519028A 2020-09-25 2021-09-24 核酸塩基編集のためのジンクフィンガー融合タンパク質 Pending JP2023542705A (ja)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US202063083662P 2020-09-25 2020-09-25
US63/083,662 2020-09-25
US202163164893P 2021-03-23 2021-03-23
US63/164,893 2021-03-23
US202163230580P 2021-08-06 2021-08-06
US63/230,580 2021-08-06
PCT/US2021/052088 WO2022067122A1 (en) 2020-09-25 2021-09-24 Zinc finger fusion proteins for nucleobase editing

Publications (2)

Publication Number Publication Date
JP2023542705A true JP2023542705A (ja) 2023-10-11
JP2023542705A5 JP2023542705A5 (https=) 2024-10-07

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JP2023519028A Pending JP2023542705A (ja) 2020-09-25 2021-09-24 核酸塩基編集のためのジンクフィンガー融合タンパク質

Country Status (9)

Country Link
US (1) US20240043829A1 (https=)
EP (1) EP4217479A1 (https=)
JP (1) JP2023542705A (https=)
KR (1) KR20230074519A (https=)
CN (1) CN116261594A (https=)
AU (1) AU2021350099A1 (https=)
CA (1) CA3196599A1 (https=)
IL (1) IL301393A (https=)
WO (1) WO2022067122A1 (https=)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2022072393A1 (en) * 2020-09-29 2022-04-07 University Of Washington Use of a double-stranded dna cytosine deaminase for mapping dna-protein interactions
JP2026501648A (ja) * 2022-12-30 2026-01-16 北京大学 塩基エディターシステムおよびその使用方法
WO2025128871A2 (en) 2023-12-13 2025-06-19 Renagade Therapeutics Management Inc. Lipid nanoparticles comprising coding rna molecules for use in gene editing and as vaccines and therapeutic agents
WO2025174765A1 (en) 2024-02-12 2025-08-21 Renagade Therapeutics Management Inc. Lipid nanoparticles comprising coding rna molecules for use in gene editing and as vaccines and therapeutic agents

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WO2020041249A1 (en) * 2018-08-23 2020-02-27 Sangamo Therapeutics, Inc. Engineered target specific base editors
JP2020521451A (ja) * 2017-05-25 2020-07-27 ザ ジェネラル ホスピタル コーポレイション 望ましくないオフターゲット塩基エディター脱アミノ化を制限するためのスプリットデアミナーゼの使用
WO2021155065A1 (en) * 2020-01-28 2021-08-05 The Broad Institute, Inc. Base editors, compositions, and methods for modifying the mitochondrial genome
WO2022060185A1 (ko) * 2020-09-18 2022-03-24 기초과학연구원 표적화된 탈아미노효소 및 이를 이용한 염기 교정
WO2022155265A2 (en) * 2021-01-12 2022-07-21 Mitolab Inc. Context-dependent, double-stranded dna-specific deaminases and uses thereof

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US5420032A (en) 1991-12-23 1995-05-30 Universitge Laval Homing endonuclease which originates from chlamydomonas eugametos and recognizes and cleaves a 15, 17 or 19 degenerate double stranded nucleotide sequence
US5792632A (en) 1992-05-05 1998-08-11 Institut Pasteur Nucleotide sequence encoding the enzyme I-SceI and the uses thereof
US6140466A (en) 1994-01-18 2000-10-31 The Scripps Research Institute Zinc finger protein derivatives and methods therefor
DE69534629D1 (de) 1994-01-18 2005-12-29 Scripps Research Inst Derivate von zinkfingerproteinen und methoden
GB9824544D0 (en) 1998-11-09 1999-01-06 Medical Res Council Screening system
USRE39229E1 (en) 1994-08-20 2006-08-08 Gendaq Limited Binding proteins for recognition of DNA
US5789538A (en) 1995-02-03 1998-08-04 Massachusetts Institute Of Technology Zinc finger proteins with high affinity new DNA binding specificities
US5925523A (en) 1996-08-23 1999-07-20 President & Fellows Of Harvard College Intraction trap assay, reagents and uses thereof
GB9710809D0 (en) 1997-05-23 1997-07-23 Medical Res Council Nucleic acid binding proteins
GB9710807D0 (en) 1997-05-23 1997-07-23 Medical Res Council Nucleic acid binding proteins
US6140081A (en) 1998-10-16 2000-10-31 The Scripps Research Institute Zinc finger binding domains for GNN
US6534261B1 (en) 1999-01-12 2003-03-18 Sangamo Biosciences, Inc. Regulation of endogenous gene expression in cells using zinc finger proteins
US6453242B1 (en) 1999-01-12 2002-09-17 Sangamo Biosciences, Inc. Selection of sites for targeting by zinc finger proteins and methods of designing zinc finger proteins to bind to preselected sites
US20020061512A1 (en) 2000-02-18 2002-05-23 Kim Jin-Soo Zinc finger domains and methods of identifying same
AU2001263155A1 (en) 2000-05-16 2001-11-26 Massachusetts Institute Of Technology Methods and compositions for interaction trap assays
JP2002060786A (ja) 2000-08-23 2002-02-26 Kao Corp 硬質表面用殺菌防汚剤
GB0108491D0 (en) 2001-04-04 2001-05-23 Gendaq Ltd Engineering zinc fingers
JP2005500061A (ja) 2001-08-20 2005-01-06 ザ スクリップス リサーチ インスティテュート Ｃｎｎについての亜鉛フィンガー結合ドメイン
CA2607104A1 (en) * 2005-05-05 2006-11-16 The Arizona Board Of Regents On Behalf Of The University Of Arizona Sequence enabled reassembly (seer) - a novel method for visualizing specific dna sequences
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JP2020521451A (ja) * 2017-05-25 2020-07-27 ザ ジェネラル ホスピタル コーポレイション 望ましくないオフターゲット塩基エディター脱アミノ化を制限するためのスプリットデアミナーゼの使用
WO2020041249A1 (en) * 2018-08-23 2020-02-27 Sangamo Therapeutics, Inc. Engineered target specific base editors
WO2021155065A1 (en) * 2020-01-28 2021-08-05 The Broad Institute, Inc. Base editors, compositions, and methods for modifying the mitochondrial genome
WO2022060185A1 (ko) * 2020-09-18 2022-03-24 기초과학연구원 표적화된 탈아미노효소 및 이를 이용한 염기 교정
WO2022155265A2 (en) * 2021-01-12 2022-07-21 Mitolab Inc. Context-dependent, double-stranded dna-specific deaminases and uses thereof

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BEVERLY Y. MOK ET AL.: ""A bacterial cytidine deaminase toxin enables CRISPR-free mitochondrial base editing"", NATURE, vol. 583, JPN6025046627, 8 July 2020 (2020-07-08), pages 631 - 637, ISSN: 0005733014 *

Also Published As

Publication number Publication date
EP4217479A1 (en) 2023-08-02
US20240043829A1 (en) 2024-02-08
AU2021350099A9 (en) 2023-07-13
WO2022067122A1 (en) 2022-03-31
IL301393A (en) 2023-05-01
CN116261594A (zh) 2023-06-13
AU2021350099A1 (en) 2023-04-27
KR20230074519A (ko) 2023-05-30
CA3196599A1 (en) 2022-03-31

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