JP2023540161A - メチル化状態の検出 - Google Patents

メチル化状態の検出 Download PDF

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Publication number
JP2023540161A
JP2023540161A JP2022580318A JP2022580318A JP2023540161A JP 2023540161 A JP2023540161 A JP 2023540161A JP 2022580318 A JP2022580318 A JP 2022580318A JP 2022580318 A JP2022580318 A JP 2022580318A JP 2023540161 A JP2023540161 A JP 2023540161A
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nucleic acid
oligonucleotide
sequence
interest
nucleotide
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JP2023540161A5 (https=
Inventor
コールディング ベンディクセン，カミラ
コーフォード ピーターセン，ラスムス
ベック クリステンセン，ウルフ
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ペンタベース エーピーエス
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6832—Enhancement of hybridisation reaction
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/113—Modifications characterised by incorporating modified backbone
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/117—Modifications characterised by incorporating modified base
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2537/00—Reactions characterised by the reaction format or use of a specific feature
    • C12Q2537/10—Reactions characterised by the reaction format or use of a specific feature the purpose or use of
    • C12Q2537/164—Methylation detection other then bisulfite or methylation sensitive restriction endonucleases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/173—Nucleic acid detection characterized by the use of physical, structural and functional properties staining/intercalating agent, e.g. ethidium bromide
    • C—CHEMISTRY; METALLURGY
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    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/118—Prognosis of disease development
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/154—Methylation markers

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Signal Processing For Digital Recording And Reproducing (AREA)
  • Sorption Type Refrigeration Machines (AREA)
  • Transition And Organic Metals Composition Catalysts For Addition Polymerization (AREA)
JP2022580318A 2020-06-29 2021-06-29 メチル化状態の検出 Pending JP2023540161A (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP20182947.0 2020-06-29
EP20182947 2020-06-29
PCT/EP2021/067880 WO2022002958A1 (en) 2020-06-29 2021-06-29 Detection of methylation status

Publications (2)

Publication Number Publication Date
JP2023540161A true JP2023540161A (ja) 2023-09-22
JP2023540161A5 JP2023540161A5 (https=) 2024-07-03

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ID=71401636

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JP2022580318A Pending JP2023540161A (ja) 2020-06-29 2021-06-29 メチル化状態の検出

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Country Link
US (1) US20250346955A1 (https=)
EP (1) EP4172361B1 (https=)
JP (1) JP2023540161A (https=)
KR (1) KR20230034959A (https=)
CN (1) CN116209775A (https=)
AU (1) AU2021302551A1 (https=)
CA (1) CA3183054A1 (https=)
WO (1) WO2022002958A1 (https=)

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2000511776A (ja) * 1996-06-03 2000-09-12 ザ ジョーンズ ホプキンス ユニバーシティー スクール オブ メディシン メチル化特異的検出
JP2006517402A (ja) * 2003-01-24 2006-07-27 ヒューマン ジェネティック シグネチャーズ ピーティーワイ リミテッド インターカレーティング核酸を用いる核酸におけるメチル化の変化を検出するためのアッセイ
JP2009529337A (ja) * 2006-03-16 2009-08-20 ペンタベイス エイピーエス 核酸、メチル化状態及び核酸の突然変異体を検出するための、シグナリングペア及び疎水性ヌクレオチドを含んだオリゴヌクレオチド、ステムレスビーコン
JP2013544507A (ja) * 2010-10-27 2013-12-19 カンティバクト・アクティーゼルスカブ インターカレータ分子を含むプローブによる標的ｄｎａ及びｒｎａの捕捉
WO2017045689A1 (en) * 2015-09-14 2017-03-23 Pentabase Aps Methods and materials for detection of mutations

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2002358464B2 (en) * 2001-12-18 2006-04-27 Human Genetic Signatures Pty Ltd Pseudonucleotide comprising an intercalator
GB0714058D0 (en) * 2007-07-19 2007-08-29 Abertec Ltd detection of methylation in nucleic acid sequences
WO2009105830A1 (en) * 2008-02-27 2009-09-03 Peter Maccallum Cancer Institute Analysing methylation specific pcr by amplicon melting

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2000511776A (ja) * 1996-06-03 2000-09-12 ザ ジョーンズ ホプキンス ユニバーシティー スクール オブ メディシン メチル化特異的検出
JP2006517402A (ja) * 2003-01-24 2006-07-27 ヒューマン ジェネティック シグネチャーズ ピーティーワイ リミテッド インターカレーティング核酸を用いる核酸におけるメチル化の変化を検出するためのアッセイ
JP2009529337A (ja) * 2006-03-16 2009-08-20 ペンタベイス エイピーエス 核酸、メチル化状態及び核酸の突然変異体を検出するための、シグナリングペア及び疎水性ヌクレオチドを含んだオリゴヌクレオチド、ステムレスビーコン
JP2013544507A (ja) * 2010-10-27 2013-12-19 カンティバクト・アクティーゼルスカブ インターカレータ分子を含むプローブによる標的ｄｎａ及びｒｎａの捕捉
WO2017045689A1 (en) * 2015-09-14 2017-03-23 Pentabase Aps Methods and materials for detection of mutations

Also Published As

Publication number Publication date
WO2022002958A1 (en) 2022-01-06
KR20230034959A (ko) 2023-03-10
US20250346955A1 (en) 2025-11-13
AU2021302551A1 (en) 2023-01-05
EP4172361B1 (en) 2024-08-07
EP4172361A1 (en) 2023-05-03
EP4172361C0 (en) 2024-08-07
CN116209775A (zh) 2023-06-02
CA3183054A1 (en) 2022-01-06

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