JP2023537341A - 複数のポリペプチドをアッセイするためのシステム及び方法 - Google Patents

複数のポリペプチドをアッセイするためのシステム及び方法 Download PDF

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Publication number
JP2023537341A
JP2023537341A JP2023507234A JP2023507234A JP2023537341A JP 2023537341 A JP2023537341 A JP 2023537341A JP 2023507234 A JP2023507234 A JP 2023507234A JP 2023507234 A JP2023507234 A JP 2023507234A JP 2023537341 A JP2023537341 A JP 2023537341A
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Prior art keywords
polypeptide
nucleic acid
binding
beads
capture moiety
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JP2023507234A
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Japanese (ja)
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JP2023537341A5 (https=
Inventor
ゴトリク,マイケル・ロイ
レイトン,カーティス・ジェームズ
バイデャナサン,パバナプレサン・プシュパギリ
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プロティリオン・バイオサイエンシズ・インコーポレイテッド
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Publication of JP2023537341A publication Critical patent/JP2023537341A/ja
Publication of JP2023537341A5 publication Critical patent/JP2023537341A5/ja
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034Isolating an individual clone by screening libraries
    • C12N15/1062Isolating an individual clone by screening libraries mRNA-Display, e.g. polypeptide and encoding template are connected covalently
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869Methods for sequencing
    • C12Q1/6874Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6834Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6818Sequencing of polypeptides
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90Enzymes; Proenzymes
    • G01N2333/91Transferases (2.)
    • G01N2333/912Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Physics & Mathematics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)
JP2023507234A 2020-07-28 2021-07-27 複数のポリペプチドをアッセイするためのシステム及び方法 Pending JP2023537341A (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US202063057754P 2020-07-28 2020-07-28
US63/057,754 2020-07-28
PCT/US2021/043297 WO2022026458A1 (en) 2020-07-28 2021-07-27 Systems and methods for assaying a plurality of polypeptides

Publications (2)

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JP2023537341A true JP2023537341A (ja) 2023-08-31
JP2023537341A5 JP2023537341A5 (https=) 2024-08-01

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JP2023507234A Pending JP2023537341A (ja) 2020-07-28 2021-07-27 複数のポリペプチドをアッセイするためのシステム及び方法

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Country Link
US (1) US20230287490A1 (https=)
EP (1) EP4189085A4 (https=)
JP (1) JP2023537341A (https=)
CN (1) CN116234927A (https=)
AU (1) AU2021318522A1 (https=)
CA (1) CA3187408A1 (https=)
WO (1) WO2022026458A1 (https=)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2025123212A1 (zh) * 2023-12-12 2025-06-19 深圳华大生命科学研究院 一种基于目标检测模型的多肽信号提取方法
WO2025165797A1 (en) * 2024-01-30 2025-08-07 Gene Company (Pty) Ltd. Hepatocyte nuclear factor four alpha antisense rna 1 targeting polynucleotide and method of use and treatment thereof

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20010046680A1 (en) * 1999-10-01 2001-11-29 Zhongping Yu Identification of polypeptides and nucleic acid molecules using linkage between DNA and polypeptide
JP2009542211A (ja) * 2006-06-30 2009-12-03 アムビト ビオスシエンセス コルポラチオン 検出可能な核酸タグ
JP2013512691A (ja) * 2009-12-07 2013-04-18 プログノシス バイオサイエンシズ インコーポレイテッド ペプチド提示アレイ
JP2015506699A (ja) * 2012-02-02 2015-03-05 インベンラ, インコーポレイテッド 生物学的に活性なポリペプチドのハイスループットスクリーニング
JP2016523978A (ja) * 2013-07-10 2016-08-12 プレジデント アンド フェローズ オブ ハーバード カレッジ 核酸−タンパク質複合体に関する組成物及び方法
WO2020095985A1 (ja) * 2018-11-07 2020-05-14 公益財団法人川崎市産業振興財団 ペプチド-核酸複合体

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8962238B2 (en) * 2007-04-05 2015-02-24 SpeedDx Pty Ltd Nucleic acid enzymes and complexes and methods for their use

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20010046680A1 (en) * 1999-10-01 2001-11-29 Zhongping Yu Identification of polypeptides and nucleic acid molecules using linkage between DNA and polypeptide
JP2009542211A (ja) * 2006-06-30 2009-12-03 アムビト ビオスシエンセス コルポラチオン 検出可能な核酸タグ
JP2013512691A (ja) * 2009-12-07 2013-04-18 プログノシス バイオサイエンシズ インコーポレイテッド ペプチド提示アレイ
JP2015506699A (ja) * 2012-02-02 2015-03-05 インベンラ, インコーポレイテッド 生物学的に活性なポリペプチドのハイスループットスクリーニング
JP2016523978A (ja) * 2013-07-10 2016-08-12 プレジデント アンド フェローズ オブ ハーバード カレッジ 核酸−タンパク質複合体に関する組成物及び方法
WO2020095985A1 (ja) * 2018-11-07 2020-05-14 公益財団法人川崎市産業振興財団 ペプチド-核酸複合体

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
GAN ET AL.: "Microbeads display of proteins using emulsion PCR and cell-free protein synthesis", BIOTECHNOL. PROG., vol. Vol. 24, Issue 5, JPN6025032279, 29 September 2008 (2008-09-29), pages 1107 - 1114, ISSN: 0005657168 *

Also Published As

Publication number Publication date
CN116234927A (zh) 2023-06-06
US20230287490A1 (en) 2023-09-14
AU2021318522A1 (en) 2023-03-23
EP4189085A1 (en) 2023-06-07
CA3187408A1 (en) 2022-02-03
WO2022026458A1 (en) 2022-02-03
EP4189085A4 (en) 2024-08-28

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