CN116234927A - 用于测定多种多肽的系统和方法 - Google Patents

用于测定多种多肽的系统和方法 Download PDF

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Publication number
CN116234927A
CN116234927A CN202180066419.5A CN202180066419A CN116234927A CN 116234927 A CN116234927 A CN 116234927A CN 202180066419 A CN202180066419 A CN 202180066419A CN 116234927 A CN116234927 A CN 116234927A
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CN
China
Prior art keywords
polypeptide
nucleic acid
ligase
microbeads
capture moiety
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Pending
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CN202180066419.5A
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English (en)
Chinese (zh)
Inventor
迈克尔·罗伊·戈特里克
柯蒂斯·詹姆斯·雷顿
帕瓦那普雷桑·普什帕吉里·威德亚纳森
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Protiryn Biosciences
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Protiryn Biosciences
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Publication of CN116234927A publication Critical patent/CN116234927A/zh
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1062—Isolating an individual clone by screening libraries mRNA-Display, e.g. polypeptide and encoding template are connected covalently
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6818—Sequencing of polypeptides
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/91—Transferases (2.)
    • G01N2333/912—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Physics & Mathematics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)
CN202180066419.5A 2020-07-28 2021-07-27 用于测定多种多肽的系统和方法 Pending CN116234927A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US202063057754P 2020-07-28 2020-07-28
US63/057,754 2020-07-28
PCT/US2021/043297 WO2022026458A1 (en) 2020-07-28 2021-07-27 Systems and methods for assaying a plurality of polypeptides

Publications (1)

Publication Number Publication Date
CN116234927A true CN116234927A (zh) 2023-06-06

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ID=80036155

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202180066419.5A Pending CN116234927A (zh) 2020-07-28 2021-07-27 用于测定多种多肽的系统和方法

Country Status (7)

Country Link
US (1) US20230287490A1 (https=)
EP (1) EP4189085A4 (https=)
JP (1) JP2023537341A (https=)
CN (1) CN116234927A (https=)
AU (1) AU2021318522A1 (https=)
CA (1) CA3187408A1 (https=)
WO (1) WO2022026458A1 (https=)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2025123212A1 (zh) * 2023-12-12 2025-06-19 深圳华大生命科学研究院 一种基于目标检测模型的多肽信号提取方法
WO2025165797A1 (en) * 2024-01-30 2025-08-07 Gene Company (Pty) Ltd. Hepatocyte nuclear factor four alpha antisense rna 1 targeting polynucleotide and method of use and treatment thereof

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20010046680A1 (en) * 1999-10-01 2001-11-29 Zhongping Yu Identification of polypeptides and nucleic acid molecules using linkage between DNA and polypeptide
MX2009000044A (es) * 2006-06-30 2009-01-23 Ambit Bios Corp Etiqueta de acido nucleico detectable.
US8962238B2 (en) * 2007-04-05 2015-02-24 SpeedDx Pty Ltd Nucleic acid enzymes and complexes and methods for their use
WO2011071943A1 (en) * 2009-12-07 2011-06-16 Prognosys Biosciences, Inc. Peptide display arrays
US9701959B2 (en) * 2012-02-02 2017-07-11 Invenra Inc. High throughput screen for biologically active polypeptides
AU2014287071A1 (en) * 2013-07-10 2016-01-28 Children's Medical Center Corporation Compositions and methods relating to nucleic acid-protein complexes
JP7416715B2 (ja) * 2018-11-07 2024-01-17 公益財団法人川崎市産業振興財団 ペプチド－核酸複合体

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JP2023537341A (ja) 2023-08-31
US20230287490A1 (en) 2023-09-14
AU2021318522A1 (en) 2023-03-23
EP4189085A1 (en) 2023-06-07
CA3187408A1 (en) 2022-02-03
WO2022026458A1 (en) 2022-02-03
EP4189085A4 (en) 2024-08-28

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