AU2021318522A1 - Systems and methods for assaying a plurality of polypeptides - Google Patents

Systems and methods for assaying a plurality of polypeptides Download PDF

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Publication number
AU2021318522A1
AU2021318522A1 AU2021318522A AU2021318522A AU2021318522A1 AU 2021318522 A1 AU2021318522 A1 AU 2021318522A1 AU 2021318522 A AU2021318522 A AU 2021318522A AU 2021318522 A AU2021318522 A AU 2021318522A AU 2021318522 A1 AU2021318522 A1 AU 2021318522A1
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AU
Australia
Prior art keywords
polypeptide
nucleic acid
bead
capture moiety
acid molecule
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
AU2021318522A
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English (en)
Inventor
Michael Roy GOTRIK
Curtis James LAYTON
Pavanapuresan Pushpagiri VAIDYANATHAN
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Protillion Biosciences Inc
Original Assignee
Protillion Biosciences Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Protillion Biosciences Inc filed Critical Protillion Biosciences Inc
Publication of AU2021318522A1 publication Critical patent/AU2021318522A1/en
Pending legal-status Critical Current

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869Methods for sequencing
    • C12Q1/6874Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034Isolating an individual clone by screening libraries
    • C12N15/1062Isolating an individual clone by screening libraries mRNA-Display, e.g. polypeptide and encoding template are connected covalently
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6834Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6818Sequencing of polypeptides
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90Enzymes; Proenzymes
    • G01N2333/91Transferases (2.)
    • G01N2333/912Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Physics & Mathematics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)
AU2021318522A 2020-07-28 2021-07-27 Systems and methods for assaying a plurality of polypeptides Pending AU2021318522A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US202063057754P 2020-07-28 2020-07-28
US63/057,754 2020-07-28
PCT/US2021/043297 WO2022026458A1 (en) 2020-07-28 2021-07-27 Systems and methods for assaying a plurality of polypeptides

Publications (1)

Publication Number Publication Date
AU2021318522A1 true AU2021318522A1 (en) 2023-03-23

Family

ID=80036155

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2021318522A Pending AU2021318522A1 (en) 2020-07-28 2021-07-27 Systems and methods for assaying a plurality of polypeptides

Country Status (7)

Country Link
US (1) US20230287490A1 (https=)
EP (1) EP4189085A4 (https=)
JP (1) JP2023537341A (https=)
CN (1) CN116234927A (https=)
AU (1) AU2021318522A1 (https=)
CA (1) CA3187408A1 (https=)
WO (1) WO2022026458A1 (https=)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2025123212A1 (zh) * 2023-12-12 2025-06-19 深圳华大生命科学研究院 一种基于目标检测模型的多肽信号提取方法
WO2025165797A1 (en) * 2024-01-30 2025-08-07 Gene Company (Pty) Ltd. Hepatocyte nuclear factor four alpha antisense rna 1 targeting polynucleotide and method of use and treatment thereof

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20010046680A1 (en) * 1999-10-01 2001-11-29 Zhongping Yu Identification of polypeptides and nucleic acid molecules using linkage between DNA and polypeptide
MX2009000044A (es) * 2006-06-30 2009-01-23 Ambit Bios Corp Etiqueta de acido nucleico detectable.
US8962238B2 (en) * 2007-04-05 2015-02-24 SpeedDx Pty Ltd Nucleic acid enzymes and complexes and methods for their use
WO2011071943A1 (en) * 2009-12-07 2011-06-16 Prognosys Biosciences, Inc. Peptide display arrays
US9701959B2 (en) * 2012-02-02 2017-07-11 Invenra Inc. High throughput screen for biologically active polypeptides
AU2014287071A1 (en) * 2013-07-10 2016-01-28 Children's Medical Center Corporation Compositions and methods relating to nucleic acid-protein complexes
JP7416715B2 (ja) * 2018-11-07 2024-01-17 公益財団法人川崎市産業振興財団 ペプチド-核酸複合体

Also Published As

Publication number Publication date
JP2023537341A (ja) 2023-08-31
CN116234927A (zh) 2023-06-06
US20230287490A1 (en) 2023-09-14
EP4189085A1 (en) 2023-06-07
CA3187408A1 (en) 2022-02-03
WO2022026458A1 (en) 2022-02-03
EP4189085A4 (en) 2024-08-28

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