JP2023515579A - 規定の条件下でのヒト多能性幹細胞からの大動脈－生殖腺－中腎様造血細胞の生成 - Google Patents

規定の条件下でのヒト多能性幹細胞からの大動脈－生殖腺－中腎様造血細胞の生成 Download PDF

Info

Publication number
JP2023515579A
JP2023515579A JP2022551294A JP2022551294A JP2023515579A JP 2023515579 A JP2023515579 A JP 2023515579A JP 2022551294 A JP2022551294 A JP 2022551294A JP 2022551294 A JP2022551294 A JP 2022551294A JP 2023515579 A JP2023515579 A JP 2023515579A
Authority
JP
Japan
Prior art keywords
cells
hematopoietic
stem cells
cell
beta
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP2022551294A
Other languages
English (en)
Japanese (ja)
Other versions
JP2023515579A5 (https=
JPWO2021173458A5 (https=
Inventor
シャオピン バオ，
ユン チャン，
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Purdue Research Foundation
Original Assignee
Purdue Research Foundation
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Purdue Research Foundation filed Critical Purdue Research Foundation
Publication of JP2023515579A publication Critical patent/JP2023515579A/ja
Publication of JP2023515579A5 publication Critical patent/JP2023515579A5/ja
Publication of JPWO2021173458A5 publication Critical patent/JPWO2021173458A5/ja
Pending legal-status Critical Current

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0634—Cells from the blood or the immune system
    • C12N5/0647—Haematopoietic stem cells; Uncommitted or multipotent progenitors
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/148—Transforming growth factor alpha [TGF-a]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/40—Regulators of development
    • C12N2501/415—Wnt; Frizzeled
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/02—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from embryonic cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/90—Substrates of biological origin, e.g. extracellular matrix, decellularised tissue

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Cell Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Virology (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
JP2022551294A 2020-02-28 2021-02-22 規定の条件下でのヒト多能性幹細胞からの大動脈－生殖腺－中腎様造血細胞の生成 Pending JP2023515579A (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US202062982992P 2020-02-28 2020-02-28
US62/982,992 2020-02-28
PCT/US2021/018964 WO2021173458A1 (en) 2020-02-28 2021-02-22 Generating aorta-gonad-mesonephros-like hematopoietic cells from human pluripotent stem cells under a defined condition

Publications (3)

Publication Number Publication Date
JP2023515579A true JP2023515579A (ja) 2023-04-13
JP2023515579A5 JP2023515579A5 (https=) 2024-02-07
JPWO2021173458A5 JPWO2021173458A5 (https=) 2024-02-07

Family

ID=77490309

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2022551294A Pending JP2023515579A (ja) 2020-02-28 2021-02-22 規定の条件下でのヒト多能性幹細胞からの大動脈－生殖腺－中腎様造血細胞の生成

Country Status (7)

Country Link
US (1) US20230097226A1 (https=)
EP (1) EP4110900A4 (https=)
JP (1) JP2023515579A (https=)
CN (1) CN115135754B (https=)
AU (1) AU2021226507A1 (https=)
CA (1) CA3171960A1 (https=)
WO (1) WO2021173458A1 (https=)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11591381B2 (en) 2020-11-30 2023-02-28 Crispr Therapeutics Ag Gene-edited natural killer cells
WO2022144632A1 (en) 2020-12-30 2022-07-07 Crispr Therapeutics Ag Compositions and methods for differentiating stem cells into nk cells
JP2024531626A (ja) * 2021-09-10 2024-08-29 スンクワン メディカル ファウンデーション 分化全能性幹細胞由来の造血幹細胞の製造方法、及び製造された造血幹細胞を利用したヒト化マウスモデル作製方法
KR20240054468A (ko) * 2022-10-18 2024-04-26 의료법인 성광의료재단 줄기세포에서 대식세포로의 분화 유도용 배지 조성물 및 이를 이용한 분화 방법
CN120303395A (zh) * 2022-12-02 2025-07-11 安徽中盛溯源生物科技有限公司 用于分化多能干细胞的方法和组合物和衍生的造血谱系细胞

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108300695A (zh) * 2018-02-07 2018-07-20 清华大学 一种人类多能干细胞向造血干细胞分化的方法及培养添加剂
WO2020051453A1 (en) * 2018-09-07 2020-03-12 Wisconsin Alumni Research Foundation Generation of hematopoietic progenitor cells from human pluripotent stem cells

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20080207594A1 (en) * 2005-05-04 2008-08-28 Davelogen Aktiengesellschaft Use of Gsk-3 Inhibitors for Preventing and Treating Pancreatic Autoimmune Disorders
CA2726164A1 (en) * 2008-05-27 2009-12-23 The Board Of Regents Of The University Of Texas System Wnt protein signalling inhibitors
WO2010033906A2 (en) * 2008-09-19 2010-03-25 President And Fellows Of Harvard College Efficient induction of pluripotent stem cells using small molecule compounds
KR101720961B1 (ko) * 2009-02-27 2017-03-29 셀룰러 다이내믹스 인터내셔널, 인코포레이티드 다능성 세포의 분화
JP5777115B2 (ja) * 2010-03-18 2015-09-09 国立大学法人京都大学 多能性幹細胞から中胚葉細胞への分化誘導法
US9896659B2 (en) * 2011-10-19 2018-02-20 Stowers Institute For Medical Research Methods, kits, and compositions for stem cell self-renewal
WO2014078414A1 (en) * 2012-11-13 2014-05-22 Joseph Wu Chemically defined production of cardiomyocytes from pluripotent stem cells
CN106661553B (zh) * 2014-05-06 2020-09-01 豪夫迈·罗氏有限公司 用于使多能干细胞分化为心肌细胞的方法
AU2016211671B2 (en) * 2015-01-26 2022-05-26 Fate Therapeutics, Inc. Methods and compositions for inducing hematopoietic cell differentiation
US10669528B2 (en) * 2015-06-25 2020-06-02 Children's Medical Center Corporation Methods and compositions relating to hematopoietic stem cell expansion, enrichment, and maintenance
CA3003152A1 (en) * 2015-11-04 2017-05-11 Fate Therapeutics, Inc. Methods and compositions for inducing hematopoietic cell differentiation
WO2017161001A1 (en) * 2016-03-15 2017-09-21 Children's Medical Center Corporation Methods and compositions relating to hematopoietic stem cell expansion
WO2017193177A1 (en) * 2016-05-13 2017-11-16 Murdoch Childrens Research Institute Haematopoietic stem/progenitor cells
SG11202006731VA (en) * 2018-01-18 2020-08-28 Agency Science Tech & Res Method for differentiation of human pluripotent stem cell lines in suspension culture

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108300695A (zh) * 2018-02-07 2018-07-20 清华大学 一种人类多能干细胞向造血干细胞分化的方法及培养添加剂
WO2020051453A1 (en) * 2018-09-07 2020-03-12 Wisconsin Alumni Research Foundation Generation of hematopoietic progenitor cells from human pluripotent stem cells

Non-Patent Citations (8)

* Cited by examiner, † Cited by third party
Title
"液体のりで造血幹細胞の増幅に成功", 東京大学[ONLINE], JPN6025053127, 30 May 2019 (2019-05-30), ISSN: 0005763252 *
CHANG Y. ET AL.: "Chemically-defined generation of human hemogenic endothelium and definitive hematopoietic progenitor", BIOMATERIAL, vol. Vol. 285:121569, JPN6025053130, June 2022 (2022-06-01), ISSN: 0005763253 *
CHOI KD. ET AL., J CLIN INVEST, vol. 119, JPN6025008990, 2009, pages 2818 - 2829, ISSN: 0005763247 *
CLARKE RL. ET AL., NAT CELL BIOL, vol. 15, JPN6025008989, 2013, pages 502 - 510, ISSN: 0005763248 *
DEGE C. ET AL., J VIS EXP, vol. Vol. 129:e5519, JPN6025008987, 2017, ISSN: 0005763250 *
LIU K. ET AL., CELL CHEM BIOL, vol. 23, JPN6025053129, 2016, pages 893 - 916, ISSN: 0005763251 *
NAFRIA M. ET AL., STAR PROTOC, vol. Vol. 1:100130, JPN6025008988, 16 October 2020 (2020-10-16), ISSN: 0005763249 *
SB 431542, TOCRIS BIOSCIENCE[ONLINE], JPN6025053131, 15 January 2018 (2018-01-15), ISSN: 0005763254 *

Also Published As

Publication number Publication date
EP4110900A1 (en) 2023-01-04
AU2021226507A1 (en) 2022-09-01
US20230097226A1 (en) 2023-03-30
CN115135754B (zh) 2026-01-20
CN115135754A (zh) 2022-09-30
WO2021173458A1 (en) 2021-09-02
EP4110900A4 (en) 2024-04-24
CA3171960A1 (en) 2021-09-02

Similar Documents

Publication Publication Date Title
JP6529541B2 (ja) 幹細胞よりナチュラルキラー細胞を発生させる方法
JP2023515579A (ja) 規定の条件下でのヒト多能性幹細胞からの大動脈－生殖腺－中腎様造血細胞の生成
US12497593B2 (en) Generation of hematopoietic progenitor cells from human pluripotent stem cells
JP5748654B2 (ja) 多能性幹細胞に由来する除核赤血球細胞を産生するための方法
US20250333701A1 (en) Compositions and methods for generating hematopoietic stem cells (hscs)
EP3777534A1 (en) Compositions and methods for ex vivo expansion of human hematopoietic stem/progenitor cells
Bastani et al. The quest for the holy grail: overcoming challenges in expanding human hematopoietic stem cells for clinical use
CN114075547A (zh) 扩增造血干细胞的方法及其组合物
CN106119200A (zh) 涉及由多能干细胞向血细胞分化的培养方法
Jiang et al. Immune cells and RBCs derived from human induced pluripotent stem cells: method, progress, prospective challenges
CN102224240A (zh) 造血干细胞生长因子
TWI891439B (zh) 低免疫原性多能幹細胞誘導分化成免疫細胞的方法
Vanuytsel et al. Recapitulating Hematopoietic Development in a Dish
Mazurek et al. In vitro derivation of megakaryocytes and platelets from induced pluripotent stem cells: developmental foundations and translational perspectives
Marcoux RET-altered Induced Pluripotent Stem Cells (iPSCs) for modeling Hematopoiesis and Non-Small Cell Lung Cancer (NSCLC)
KR20240080290A (ko) 줄기세포에서 감마델타 t세포로의 분화 유도용 배지 조성물 및 이를 이용한 분화 방법
Dijkhuis Generation of autologous hematopoietic stem cells from induced pluripotent stem cells
CN121759404A (zh) 一种制备γδT细胞的试剂组合或试剂盒及其应用
Lu et al. Cell Therapy for Blood Substitutes
HK40017506A (en) Method for developing natural killer cells from stem cells
HK1073131A (en) Method of amplyfying hematopoietic stem cells

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20240130

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20240130

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20250305

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20250529

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20250905

A02 Decision of refusal

Free format text: JAPANESE INTERMEDIATE CODE: A02

Effective date: 20260105