CN115135754B - 在限定条件下从人多能干细胞生成主动脉-性腺-中肾样造血细胞 - Google Patents

在限定条件下从人多能干细胞生成主动脉-性腺-中肾样造血细胞

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Publication number
CN115135754B
CN115135754B CN202180017319.3A CN202180017319A CN115135754B CN 115135754 B CN115135754 B CN 115135754B CN 202180017319 A CN202180017319 A CN 202180017319A CN 115135754 B CN115135754 B CN 115135754B
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cells
hematopoietic
stem cells
cell
human
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CN115135754A (zh
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包小平
常贇
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Purdue Research Foundation
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Purdue Research Foundation
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0634—Cells from the blood or the immune system
    • C12N5/0647—Haematopoietic stem cells; Uncommitted or multipotent progenitors
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/148—Transforming growth factor alpha [TGF-a]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/40—Regulators of development
    • C12N2501/415—Wnt; Frizzeled
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/02—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from embryonic cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/90—Substrates of biological origin, e.g. extracellular matrix, decellularised tissue

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Hematology (AREA)
  • Immunology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Cell Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Epidemiology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
CN202180017319.3A 2020-02-28 2021-02-22 在限定条件下从人多能干细胞生成主动脉-性腺-中肾样造血细胞 Active CN115135754B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US202062982992P 2020-02-28 2020-02-28
US62/982992 2020-02-28
PCT/US2021/018964 WO2021173458A1 (en) 2020-02-28 2021-02-22 Generating aorta-gonad-mesonephros-like hematopoietic cells from human pluripotent stem cells under a defined condition

Publications (2)

Publication Number Publication Date
CN115135754A CN115135754A (zh) 2022-09-30
CN115135754B true CN115135754B (zh) 2026-01-20

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CN202180017319.3A Active CN115135754B (zh) 2020-02-28 2021-02-22 在限定条件下从人多能干细胞生成主动脉-性腺-中肾样造血细胞

Country Status (7)

Country Link
US (1) US20230097226A1 (https=)
EP (1) EP4110900A4 (https=)
JP (1) JP2023515579A (https=)
CN (1) CN115135754B (https=)
AU (1) AU2021226507A1 (https=)
CA (1) CA3171960A1 (https=)
WO (1) WO2021173458A1 (https=)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11459372B2 (en) 2020-11-30 2022-10-04 Crispr Therapeutics Ag Gene-edited natural killer cells
WO2022144632A1 (en) 2020-12-30 2022-07-07 Crispr Therapeutics Ag Compositions and methods for differentiating stem cells into nk cells
WO2023038436A1 (ko) * 2021-09-10 2023-03-16 의료법인 성광의료재단 전분화능 줄기세포 유래 조혈모세포 제조방법 및 상기 제조된 조혈모세포를 이용한 인간화 마우스 모델 제작방법
KR20240054468A (ko) * 2022-10-18 2024-04-26 의료법인 성광의료재단 줄기세포에서 대식세포로의 분화 유도용 배지 조성물 및 이를 이용한 분화 방법
US20240352419A1 (en) * 2022-12-02 2024-10-24 Nuwacell Biotechnologies Co., Ltd. Methods and compositions for differentiation of pluripotent stem cells and derived hematopoietic lineage cells

Citations (1)

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Publication number Priority date Publication date Assignee Title
WO2019143292A1 (en) * 2018-01-18 2019-07-25 Agency For Science, Technology And Research Method for differentiation of human pluripotent stem cell lines in suspension culture

Family Cites Families (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20080207594A1 (en) * 2005-05-04 2008-08-28 Davelogen Aktiengesellschaft Use of Gsk-3 Inhibitors for Preventing and Treating Pancreatic Autoimmune Disorders
JP2011521958A (ja) * 2008-05-27 2011-07-28 ザ ボード オブ リージェンツ オブ ザ ユニバーシティー オブ テキサス システム Ｗｎｔタンパク質シグナル伝達阻害剤
US20120021519A1 (en) * 2008-09-19 2012-01-26 Presidents And Fellows Of Harvard College Efficient induction of pluripotent stem cells using small molecule compounds
KR101720961B1 (ko) * 2009-02-27 2017-03-29 셀룰러 다이내믹스 인터내셔널, 인코포레이티드 다능성 세포의 분화
US8652845B2 (en) * 2010-03-18 2014-02-18 Kyoto University Method for producing mesodermal cells by culturing under adherent conditions and without co-culture with cells from a different species in a serum-free medium
WO2013059357A1 (en) * 2011-10-19 2013-04-25 Stowers Institute For Medical Research Methods, kits, and compositions for stem cell self-renewal
WO2014078414A1 (en) * 2012-11-13 2014-05-22 Joseph Wu Chemically defined production of cardiomyocytes from pluripotent stem cells
RU2016146567A (ru) * 2014-05-06 2018-06-08 Ф. Хоффманн-Ля Рош Аг Способ дифференцировки плюрипотентных стволовых клеток в кардиомиоциты
WO2016123100A1 (en) * 2015-01-26 2016-08-04 Fate Therapeutics, Inc. Methods and compositions for inducing hematopoietic cell differentiation
US10669528B2 (en) * 2015-06-25 2020-06-02 Children's Medical Center Corporation Methods and compositions relating to hematopoietic stem cell expansion, enrichment, and maintenance
SG11201803145RA (en) * 2015-11-04 2018-05-30 Fate Therapeutics Inc Methods and compositions for inducing hematopoietic cell differentiation
EP4049665B1 (en) * 2016-03-15 2025-03-12 The Children's Medical Center Corporation Methods and compositions relating to hematopoietic stem cell expansion
CA3023134A1 (en) * 2016-05-13 2017-11-16 Murdoch Childrens Research Institute Haematopoietic stem/progenitor cells
CN108300695B (zh) * 2018-02-07 2021-08-06 清华大学 一种人类多能干细胞向造血干细胞分化的方法及培养添加剂
AU2019336221B2 (en) * 2018-09-07 2025-05-15 Wisconsin Alumni Research Foundation Generation of hematopoietic progenitor cells from human pluripotent stem cells

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2019143292A1 (en) * 2018-01-18 2019-07-25 Agency For Science, Technology And Research Method for differentiation of human pluripotent stem cell lines in suspension culture

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
"Chemically-defined generation of human hemogenic endothelium and definitive hematopoietic progenitor cells";Chang Y 等;BIOMATERIALS;20220606;第285卷;121569号, 第1-17页 *

Also Published As

Publication number Publication date
EP4110900A1 (en) 2023-01-04
CA3171960A1 (en) 2021-09-02
CN115135754A (zh) 2022-09-30
AU2021226507A1 (en) 2022-09-01
JP2023515579A (ja) 2023-04-13
EP4110900A4 (en) 2024-04-24
US20230097226A1 (en) 2023-03-30
WO2021173458A1 (en) 2021-09-02

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