JP2023514749A - 統合型インサイチュ空間的アッセイのための方法および組成物 - Google Patents
統合型インサイチュ空間的アッセイのための方法および組成物 Download PDFInfo
- Publication number
- JP2023514749A JP2023514749A JP2022550866A JP2022550866A JP2023514749A JP 2023514749 A JP2023514749 A JP 2023514749A JP 2022550866 A JP2022550866 A JP 2022550866A JP 2022550866 A JP2022550866 A JP 2022550866A JP 2023514749 A JP2023514749 A JP 2023514749A
- Authority
- JP
- Japan
- Prior art keywords
- nucleic acid
- probes
- capture
- probe
- substrate
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6841—In situ hybridisation
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
- C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Genetics & Genomics (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
- Heterocyclic Carbon Compounds Containing A Hetero Ring Having Oxygen Or Sulfur (AREA)
- Plural Heterocyclic Compounds (AREA)
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US202062980078P | 2020-02-21 | 2020-02-21 | |
| US62/980,078 | 2020-02-21 | ||
| US202063111518P | 2020-11-09 | 2020-11-09 | |
| US63/111,518 | 2020-11-09 | ||
| PCT/US2021/018826 WO2021168287A1 (en) | 2020-02-21 | 2021-02-19 | Methods and compositions for integrated in situ spatial assay |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2023514749A true JP2023514749A (ja) | 2023-04-07 |
| JP2023514749A5 JP2023514749A5 (enExample) | 2024-02-28 |
Family
ID=74871841
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2022550866A Pending JP2023514749A (ja) | 2020-02-21 | 2021-02-19 | 統合型インサイチュ空間的アッセイのための方法および組成物 |
Country Status (8)
| Country | Link |
|---|---|
| US (2) | US12173360B2 (enExample) |
| EP (2) | EP4107285B1 (enExample) |
| JP (1) | JP2023514749A (enExample) |
| CN (1) | CN115698324A (enExample) |
| AU (1) | AU2021225020A1 (enExample) |
| CA (1) | CA3168202A1 (enExample) |
| ES (1) | ES2993641T3 (enExample) |
| WO (1) | WO2021168287A1 (enExample) |
Families Citing this family (144)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2019068880A1 (en) | 2017-10-06 | 2019-04-11 | Cartana Ab | RNA MATRIX LIGATION |
| CN112243463B (zh) | 2018-04-09 | 2024-12-13 | 小利兰·斯坦福大学托管委员会 | 原位基因测序方法 |
| US11519033B2 (en) | 2018-08-28 | 2022-12-06 | 10X Genomics, Inc. | Method for transposase-mediated spatial tagging and analyzing genomic DNA in a biological sample |
| EP3894585A2 (en) | 2018-12-10 | 2021-10-20 | 10X Genomics, Inc. | Generating capture probes for spatial analysis |
| US11926867B2 (en) | 2019-01-06 | 2024-03-12 | 10X Genomics, Inc. | Generating capture probes for spatial analysis |
| US11649485B2 (en) | 2019-01-06 | 2023-05-16 | 10X Genomics, Inc. | Generating capture probes for spatial analysis |
| EP3976820A1 (en) | 2019-05-30 | 2022-04-06 | 10X Genomics, Inc. | Methods of detecting spatial heterogeneity of a biological sample |
| CN119220641A (zh) | 2019-05-31 | 2024-12-31 | 10X基因组学有限公司 | 检测目标核酸分子的方法 |
| CA3146081A1 (en) * | 2019-07-30 | 2021-02-04 | Huy Quoc NGUYEN | Methods and compositions related to target analysis |
| EP4025711A2 (en) | 2019-11-08 | 2022-07-13 | 10X Genomics, Inc. | Enhancing specificity of analyte binding |
| WO2021091611A1 (en) | 2019-11-08 | 2021-05-14 | 10X Genomics, Inc. | Spatially-tagged analyte capture agents for analyte multiplexing |
| GB201919029D0 (en) | 2019-12-20 | 2020-02-05 | Cartana Ab | Method of detecting an analyte |
| GB201919032D0 (en) | 2019-12-20 | 2020-02-05 | Cartana Ab | Method of detecting an analyte |
| EP4081656A1 (en) | 2019-12-23 | 2022-11-02 | 10X Genomics, Inc. | Compositions and methods for using fixed biological samples in partition-based assays |
| CN121022991A (zh) | 2019-12-23 | 2025-11-28 | 10X基因组学有限公司 | 使用rna模板化连接进行空间分析的方法 |
| EP4752521A3 (en) | 2019-12-23 | 2026-08-12 | 10X Genomics, Inc. | Reversible fixing reagents and methods of use thereof |
| US12365942B2 (en) | 2020-01-13 | 2025-07-22 | 10X Genomics, Inc. | Methods of decreasing background on a spatial array |
| US12405264B2 (en) | 2020-01-17 | 2025-09-02 | 10X Genomics, Inc. | Electrophoretic system and method for analyte capture |
| US11732299B2 (en) | 2020-01-21 | 2023-08-22 | 10X Genomics, Inc. | Spatial assays with perturbed cells |
| US11702693B2 (en) | 2020-01-21 | 2023-07-18 | 10X Genomics, Inc. | Methods for printing cells and generating arrays of barcoded cells |
| US20210230681A1 (en) | 2020-01-24 | 2021-07-29 | 10X Genomics, Inc. | Methods for spatial analysis using proximity ligation |
| US11821035B1 (en) | 2020-01-29 | 2023-11-21 | 10X Genomics, Inc. | Compositions and methods of making gene expression libraries |
| US12076701B2 (en) | 2020-01-31 | 2024-09-03 | 10X Genomics, Inc. | Capturing oligonucleotides in spatial transcriptomics |
| US11898205B2 (en) | 2020-02-03 | 2024-02-13 | 10X Genomics, Inc. | Increasing capture efficiency of spatial assays |
| US12110548B2 (en) | 2020-02-03 | 2024-10-08 | 10X Genomics, Inc. | Bi-directional in situ analysis |
| US12110541B2 (en) | 2020-02-03 | 2024-10-08 | 10X Genomics, Inc. | Methods for preparing high-resolution spatial arrays |
| US11732300B2 (en) | 2020-02-05 | 2023-08-22 | 10X Genomics, Inc. | Increasing efficiency of spatial analysis in a biological sample |
| US12129516B2 (en) | 2020-02-07 | 2024-10-29 | 10X Genomics, Inc. | Quantitative and automated permeabilization performance evaluation for spatial transcriptomics |
| US11835462B2 (en) | 2020-02-11 | 2023-12-05 | 10X Genomics, Inc. | Methods and compositions for partitioning a biological sample |
| US12399123B1 (en) | 2020-02-14 | 2025-08-26 | 10X Genomics, Inc. | Spatial targeting of analytes |
| US12281357B1 (en) | 2020-02-14 | 2025-04-22 | 10X Genomics, Inc. | In situ spatial barcoding |
| WO2021167986A1 (en) | 2020-02-17 | 2021-08-26 | 10X Genomics, Inc. | In situ analysis of chromatin interaction |
| EP4107285B1 (en) | 2020-02-21 | 2024-10-09 | 10X Genomics, Inc. | Methods and compositions for integrated in situ spatial assay |
| CN116157533A (zh) | 2020-02-21 | 2023-05-23 | 10X基因组学有限公司 | 使用杂交方法捕获遗传靶标 |
| US11891654B2 (en) | 2020-02-24 | 2024-02-06 | 10X Genomics, Inc. | Methods of making gene expression libraries |
| US11926863B1 (en) | 2020-02-27 | 2024-03-12 | 10X Genomics, Inc. | Solid state single cell method for analyzing fixed biological cells |
| US11768175B1 (en) | 2020-03-04 | 2023-09-26 | 10X Genomics, Inc. | Electrophoretic methods for spatial analysis |
| US12188085B2 (en) | 2020-03-05 | 2025-01-07 | 10X Genomics, Inc. | Three-dimensional spatial transcriptomics with sequencing readout |
| EP4139485B1 (en) | 2020-04-22 | 2023-09-06 | 10X Genomics, Inc. | Methods for spatial analysis using targeted rna depletion |
| US12416603B2 (en) | 2020-05-19 | 2025-09-16 | 10X Genomics, Inc. | Electrophoresis cassettes and instrumentation |
| EP4153775B1 (en) | 2020-05-22 | 2024-07-24 | 10X Genomics, Inc. | Simultaneous spatio-temporal measurement of gene expression and cellular activity |
| AU2021275906A1 (en) | 2020-05-22 | 2022-12-22 | 10X Genomics, Inc. | Spatial analysis to detect sequence variants |
| WO2021242834A1 (en) | 2020-05-26 | 2021-12-02 | 10X Genomics, Inc. | Method for resetting an array |
| US12265079B1 (en) | 2020-06-02 | 2025-04-01 | 10X Genomics, Inc. | Systems and methods for detecting analytes from captured single biological particles |
| EP4025692A2 (en) | 2020-06-02 | 2022-07-13 | 10X Genomics, Inc. | Nucleic acid library methods |
| EP4600376A3 (en) | 2020-06-02 | 2025-10-22 | 10X Genomics, Inc. | Spatial transcriptomics for antigen-receptors |
| US12031177B1 (en) | 2020-06-04 | 2024-07-09 | 10X Genomics, Inc. | Methods of enhancing spatial resolution of transcripts |
| EP4421186B1 (en) | 2020-06-08 | 2025-08-13 | 10X Genomics, Inc. | Methods of determining a surgical margin and methods of use thereof |
| EP4446430A3 (en) | 2020-06-10 | 2024-12-18 | 10X Genomics, Inc. | Methods for determining a location of an analyte in a biological sample |
| EP4164796A4 (en) | 2020-06-10 | 2024-03-06 | 10x Genomics, Inc. | Fluid delivery methods |
| US12435363B1 (en) | 2020-06-10 | 2025-10-07 | 10X Genomics, Inc. | Materials and methods for spatial transcriptomics |
| US12209273B2 (en) | 2020-06-12 | 2025-01-28 | 10X Genomics, Inc. | Nucleic acid assays using click chemistry bioconjugation |
| EP4450639B1 (en) | 2020-06-25 | 2025-10-15 | 10X Genomics, Inc. | Spatial analysis of dna methylation |
| US12209280B1 (en) | 2020-07-06 | 2025-01-28 | 10X Genomics, Inc. | Methods of identifying abundance and location of an analyte in a biological sample using second strand synthesis |
| US11981960B1 (en) | 2020-07-06 | 2024-05-14 | 10X Genomics, Inc. | Spatial analysis utilizing degradable hydrogels |
| US11761038B1 (en) | 2020-07-06 | 2023-09-19 | 10X Genomics, Inc. | Methods for identifying a location of an RNA in a biological sample |
| US11492662B2 (en) * | 2020-08-06 | 2022-11-08 | Singular Genomics Systems, Inc. | Methods for in situ transcriptomics and proteomics |
| US11434525B2 (en) | 2020-08-06 | 2022-09-06 | Singular Genomics Systems, Inc. | Spatial sequencing |
| US12553898B1 (en) | 2020-08-10 | 2026-02-17 | 10X Genomics, Inc. | Fluorescent hybridization of antibody-oligonucleotide for multiplexing and signal amplification |
| US12297499B2 (en) | 2020-08-17 | 2025-05-13 | 10X Genomics, Inc. | Multicomponent nucleic acid probes for sample analysis |
| US11981958B1 (en) | 2020-08-20 | 2024-05-14 | 10X Genomics, Inc. | Methods for spatial analysis using DNA capture |
| CN116194590A (zh) | 2020-09-18 | 2023-05-30 | 10X基因组学有限公司 | 样品处理设备和图像配准方法 |
| US11926822B1 (en) | 2020-09-23 | 2024-03-12 | 10X Genomics, Inc. | Three-dimensional spatial analysis |
| US12071667B2 (en) | 2020-11-04 | 2024-08-27 | 10X Genomics, Inc. | Sequence analysis using meta-stable nucleic acid molecules |
| US11827935B1 (en) | 2020-11-19 | 2023-11-28 | 10X Genomics, Inc. | Methods for spatial analysis using rolling circle amplification and detection probes |
| EP4012046A1 (en) | 2020-12-11 | 2022-06-15 | 10X Genomics, Inc. | Methods and compositions for multimodal in situ analysis |
| WO2022140028A1 (en) | 2020-12-21 | 2022-06-30 | 10X Genomics, Inc. | Methods, compositions, and systems for capturing probes and/or barcodes |
| US12624392B2 (en) | 2020-12-30 | 2026-05-12 | 10X Genomics, Inc. | Molecular array generation using photoresist |
| US12060603B2 (en) | 2021-01-19 | 2024-08-13 | 10X Genomics, Inc. | Methods for internally controlled in situ assays using padlock probes |
| EP4738279A3 (en) | 2021-02-19 | 2026-05-13 | The Broad Institute Inc. | Multi-scale spatial transcriptomics analysis |
| ES2989523T3 (es) | 2021-02-19 | 2024-11-26 | 10X Genomics Inc | Método de uso de un dispositivo modular de soporte para ensayos |
| US12275984B2 (en) | 2021-03-02 | 2025-04-15 | 10X Genomics, Inc. | Sequential hybridization and quenching |
| EP4524261B1 (en) | 2021-03-18 | 2026-07-22 | 10X Genomics, Inc. | Multiplex capture of gene and protein expression from a biological sample |
| EP4305196B1 (en) | 2021-04-14 | 2025-04-02 | 10X Genomics, Inc. | Methods of measuring mislocalization of an analyte |
| WO2022235764A1 (en) | 2021-05-05 | 2022-11-10 | Singular Genomics Systems, Inc. | Multiomic analysis device and methods of use thereof |
| EP4320271B1 (en) | 2021-05-06 | 2025-03-19 | 10X Genomics, Inc. | Methods for increasing resolution of spatial analysis |
| EP4334471A1 (en) | 2021-05-07 | 2024-03-13 | The Broad Institute Inc. | Spatiotemporally resolved transcriptomics at subcellular resolution |
| JP2024522350A (ja) * | 2021-05-28 | 2024-06-18 | ザ ブロード インスティテュート,インコーポレーテッド | 転写状態およびタンパク質組織像の共マッピング |
| ES3030033T3 (en) | 2021-06-03 | 2025-06-26 | 10X Genomics Inc | Methods, compositions, kits, and systems for enhancing analyte capture for spatial analysis |
| CA3223257A1 (en) * | 2021-06-24 | 2022-12-29 | Olof John Ericsson | Spatial analysis of a planar biological sample |
| EP4326900B1 (en) | 2021-07-30 | 2024-09-04 | 10X Genomics, Inc. | Methods and compositions for synchronizing reactions in situ |
| US12139751B2 (en) | 2021-07-30 | 2024-11-12 | 10X Genomics, Inc. | Circularizable probes for in situ analysis |
| US12553805B2 (en) | 2021-08-02 | 2026-02-17 | 10X Genomics, Inc. | Methods of preserving a biological sample |
| US12529096B2 (en) | 2021-08-03 | 2026-01-20 | 10X Genomics, Inc. | Stabilization and/or compaction of nucleic acid structures |
| US12391984B2 (en) | 2021-08-03 | 2025-08-19 | 10X Genomics, Inc. | Compositions and methods for rolling circle amplification |
| EP4381095A1 (en) | 2021-08-03 | 2024-06-12 | 10X Genomics, Inc. | Nucleic acid concatemers and methods for stabilizing and/or compacting the same |
| US12460251B2 (en) | 2021-08-03 | 2025-11-04 | 10X Genomics, Inc. | Stabilization and/or compaction of nucleic acid molecules |
| US12435364B2 (en) | 2021-08-16 | 2025-10-07 | 10X Genomics, Inc. | Probes comprising a split barcode region and methods of use |
| WO2023034489A1 (en) | 2021-09-01 | 2023-03-09 | 10X Genomics, Inc. | Methods, compositions, and kits for blocking a capture probe on a spatial array |
| JP2024538546A (ja) * | 2021-09-23 | 2024-10-23 | スタンダード バイオツールズ カナダ インコーポレイテッド | 懸濁液マスサイトメトリーのための空間バーコード化 関連出願の相互参照 本出願は、参照により本明細書に組み込まれる、2021年9月23日に出願された米国仮出願第63/247,611号の利益および優先権を主張する。 |
| WO2023077109A2 (en) * | 2021-10-29 | 2023-05-04 | Singular Genomics Systems, Inc. | Split oligonucleotide partner probes |
| EP4430398B1 (en) * | 2021-11-10 | 2025-12-31 | 10X Genomics, Inc. | METHODS FOR IDENTIFYING ANTIGEN-BINDING MOLECULES |
| EP4419707A1 (en) | 2021-11-10 | 2024-08-28 | 10X Genomics, Inc. | Methods, compositions, and kits for determining the location of an analyte in a biological sample |
| EP4419709A4 (en) * | 2021-11-23 | 2025-02-26 | Pleno, Inc. | MULTIPLEXED DETECTION OF TARGET BIOMOLECULES |
| EP4305195A2 (en) * | 2021-12-01 | 2024-01-17 | 10X Genomics, Inc. | Methods, compositions, and systems for improved in situ detection of analytes and spatial analysis |
| WO2023102240A1 (en) * | 2021-12-03 | 2023-06-08 | California Institute Of Technology | Method of mapping spatial distributions of cellular components |
| US20250093339A1 (en) * | 2021-12-15 | 2025-03-20 | BioLegend, Inc. | Compositions and methods for enhanced sample multiplexing |
| EP4441711A1 (en) | 2021-12-20 | 2024-10-09 | 10X Genomics, Inc. | Self-test for pathology/histology slide imaging device |
| US20250043273A1 (en) * | 2021-12-24 | 2025-02-06 | Bgi Shenzhen | Dna library sequence for increasing number of chip probes and application thereof |
| WO2023137421A1 (en) * | 2022-01-17 | 2023-07-20 | University Of Washington | Oligo-mediated deposition of functional molecules for spatially resolved genomics, transcriptomics, and proteomics |
| US12674196B2 (en) | 2022-01-18 | 2026-07-07 | 10X Genomics, Inc. | Methods and compositions for improved probe specificity |
| EP4733410A3 (en) | 2022-01-21 | 2026-05-27 | 10X Genomics, Inc. | Multiple readout signals for analyzing a sample |
| US20250163501A1 (en) * | 2022-02-01 | 2025-05-22 | 10X Genomics, Inc. | Methods, kits, compositions, and systems for spatial analysis |
| US20250179564A1 (en) * | 2022-02-08 | 2025-06-05 | The Trustees Of The University Of Pennsylvania | Methods for Rapid, Scalable, Amplified Nucleic Acid Detection In Situ |
| WO2023172915A1 (en) | 2022-03-08 | 2023-09-14 | 10X Genomics, Inc. | In situ code design methods for minimizing optical crowding |
| US20250188521A1 (en) | 2022-03-10 | 2025-06-12 | Singular Genomics Systems, Inc. | Nucleic acid delivery scaffolds |
| US12637706B2 (en) | 2022-03-23 | 2026-05-26 | 10X Genomics, Inc. | Methods and compositions related to microsphere surface gelation |
| US12703879B2 (en) | 2022-03-29 | 2026-08-11 | 10X Genomics, Inc. | RNase H assisted in situ rolling circle amplification |
| CN119032271A (zh) | 2022-04-01 | 2024-11-26 | 10X基因组学有限公司 | 用于靶向掩蔽自发荧光的组合物和方法 |
| US12687494B2 (en) | 2022-04-04 | 2026-07-21 | 10X Genomics, Inc. | Systems and methods for nearly isotropic optical resolution using tilted structured illumination microscopy |
| WO2023196526A1 (en) * | 2022-04-06 | 2023-10-12 | 10X Genomics, Inc. | Methods for multiplex cell analysis |
| US20250251403A1 (en) * | 2022-04-12 | 2025-08-07 | 10X Genomics, Inc. | Compositions and methods for generating and characterizing recombinant antigen binding molecules |
| US20240002902A1 (en) | 2022-05-06 | 2024-01-04 | 10X Genomics, Inc. | Analysis of antigen and antigen receptor interactions |
| US12559791B2 (en) | 2022-05-06 | 2026-02-24 | 10X Genomics, Inc. | Methods and compositions for in situ analysis of V(D)J sequences |
| CN119213143A (zh) | 2022-05-11 | 2024-12-27 | 10X基因组学有限公司 | 用于原位测序的组合物和方法 |
| US20240035071A1 (en) | 2022-06-17 | 2024-02-01 | 10X Genomics, Inc. | Catalytic de-crosslinking of samples for in situ analysis |
| WO2023250077A1 (en) * | 2022-06-22 | 2023-12-28 | 10X Genomics, Inc. | Methods, compositions, and systems for capturing probes and/or barcodes |
| US20240076722A1 (en) * | 2022-06-29 | 2024-03-07 | 10X Genomics, Inc. | Compositions and methods for oligonucleotide inversion on arrays |
| US12692484B2 (en) | 2022-08-12 | 2026-07-28 | 10X Genomics, Inc. | PUMA1 polymerases and uses thereof |
| CA3264896A1 (en) | 2022-08-15 | 2024-02-22 | Element Biosciences, Inc. | SPATIAL RESOLUTION SURFACE CAPTURE OF NUCLEIC ACIDS |
| US12116626B2 (en) | 2022-08-16 | 2024-10-15 | 10X Genomics, Inc. | AP50 polymerases and uses thereof |
| EP4339297A1 (en) * | 2022-09-19 | 2024-03-20 | Miltenyi Biotec B.V. & Co. KG | Targeted detection of single nucleotide polymorphisms (snp) directly on tissue |
| CN117512070B (zh) * | 2022-10-14 | 2024-08-16 | 深圳赛陆医疗科技有限公司 | 在载体上生成探针的方法和样品中靶物质的检测方法 |
| WO2024086776A1 (en) * | 2022-10-20 | 2024-04-25 | 10X Genomics, Inc. | Hybrid microbead arrays |
| EP4361286A1 (en) * | 2022-10-25 | 2024-05-01 | Miltenyi Biotec B.V. & Co. KG | Method for determining the spatial localisation of rna strands on a surface |
| EP4685239A1 (en) | 2022-11-09 | 2026-01-28 | 10X Genomics, Inc. | Methods, compositions, and kits for determining the location of multiple analytes in a biological sample |
| WO2024141901A1 (en) * | 2022-12-28 | 2024-07-04 | Moleculent Ab | Heat-based transfer of reaction products made in situ to a planar support |
| CN116042781B (zh) * | 2023-02-16 | 2026-04-21 | 中国科学技术大学 | 一种原位高通量检测rna与核糖体互作的方法及应用 |
| CN117385477B (zh) * | 2023-03-10 | 2024-09-20 | 深圳赛陆医疗科技有限公司 | 用于空间转录组学测序的芯片及其制备方法、空间转录组学测序方法 |
| WO2024229260A1 (en) * | 2023-05-03 | 2024-11-07 | 10X Genomics, Inc. | Methods and compositions for spatial assay |
| EP4720332A1 (en) | 2023-06-01 | 2026-04-08 | Singular Genomics Systems, Inc. | Methods and probes for detecting polynucleotide sequences in cells and tissues |
| EP4474484A1 (en) * | 2023-06-07 | 2024-12-11 | Leica Microsystems CMS GmbH | Method for analysing a biological sample |
| WO2024256715A1 (en) * | 2023-06-16 | 2024-12-19 | Resolve Biosciences Gmbh | Reagent for in situ hybridization quality control |
| WO2025029831A1 (en) | 2023-07-31 | 2025-02-06 | 10X Genomics, Inc. | Methods and systems for targeted rna cleavage and target rna-primed rolling circle amplification |
| US20250154579A1 (en) * | 2023-11-12 | 2025-05-15 | Interplay Bio | System and method for high throughput screening of small molecule-protein interactions |
| WO2025129663A1 (zh) * | 2023-12-22 | 2025-06-26 | 深圳华大生命科学研究院 | Rna-蛋白质互作的检测方法 |
| WO2025138284A1 (zh) * | 2023-12-29 | 2025-07-03 | 深圳华大生命科学研究院 | 用于检测核酸空间信息的阵列及检测方法 |
| WO2025189030A1 (en) | 2024-03-07 | 2025-09-12 | Stellaromics, Inc. | Methods and systems for volumetric imaging |
| WO2025235538A1 (en) * | 2024-05-06 | 2025-11-13 | The Board Of Trustees Of The Leland Stanford Junior University | Ai-assisted label-free optical platform to characterize nano and micro-vesicles and biological tissues |
| WO2025255457A1 (en) * | 2024-06-07 | 2025-12-11 | 10X Genomics, Inc. | Compositions and methods for improved on-target spatial profiling |
| WO2026037951A1 (en) * | 2024-08-16 | 2026-02-19 | Resolve Biosciences Gmbh | Compositions and methods for spatial analysis of multicell suspensions |
| WO2026039842A2 (en) * | 2024-08-16 | 2026-02-19 | University Of Florida Research Foundation, Incorporated | Buffer exchange free, highly multiplexed bio-imaging with in situ dna strand displacement |
| WO2026044122A1 (en) * | 2024-08-22 | 2026-02-26 | Becton, Dickinson And Company | Non-sequencing flow cytometry-based method for bead assay efficiency |
| CN120700172A (zh) * | 2025-08-14 | 2025-09-26 | 北京大学成都前沿交叉生物技术研究院 | 利用探针捕获的16S/18S rRNA鉴定细菌/真菌在生物组织中的空间分布的方法 |
Family Cites Families (237)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4965188A (en) | 1986-08-22 | 1990-10-23 | Cetus Corporation | Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme |
| US4683202A (en) | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
| US4683195A (en) | 1986-01-30 | 1987-07-28 | Cetus Corporation | Process for amplifying, detecting, and/or-cloning nucleic acid sequences |
| US4800159A (en) | 1986-02-07 | 1989-01-24 | Cetus Corporation | Process for amplifying, detecting, and/or cloning nucleic acid sequences |
| US5525464A (en) | 1987-04-01 | 1996-06-11 | Hyseq, Inc. | Method of sequencing by hybridization of oligonucleotide probes |
| US5472881A (en) | 1992-11-12 | 1995-12-05 | University Of Utah Research Foundation | Thiol labeling of DNA for attachment to gold surfaces |
| US5874239A (en) | 1993-07-30 | 1999-02-23 | Affymax Technologies N.V. | Biotinylation of proteins |
| US5610287A (en) | 1993-12-06 | 1997-03-11 | Molecular Tool, Inc. | Method for immobilizing nucleic acid molecules |
| US5681697A (en) | 1993-12-08 | 1997-10-28 | Chiron Corporation | Solution phase nucleic acid sandwich assays having reduced background noise and kits therefor |
| US5512462A (en) | 1994-02-25 | 1996-04-30 | Hoffmann-La Roche Inc. | Methods and reagents for the polymerase chain reaction amplification of long DNA sequences |
| US5552278A (en) | 1994-04-04 | 1996-09-03 | Spectragen, Inc. | DNA sequencing by stepwise ligation and cleavage |
| US5807522A (en) | 1994-06-17 | 1998-09-15 | The Board Of Trustees Of The Leland Stanford Junior University | Methods for fabricating microarrays of biological samples |
| US5846719A (en) | 1994-10-13 | 1998-12-08 | Lynx Therapeutics, Inc. | Oligonucleotide tags for sorting and identification |
| US5750341A (en) | 1995-04-17 | 1998-05-12 | Lynx Therapeutics, Inc. | DNA sequencing by parallel oligonucleotide extensions |
| US5854033A (en) | 1995-11-21 | 1998-12-29 | Yale University | Rolling circle replication reporter systems |
| GB9620209D0 (en) | 1996-09-27 | 1996-11-13 | Cemu Bioteknik Ab | Method of sequencing DNA |
| GB9626815D0 (en) | 1996-12-23 | 1997-02-12 | Cemu Bioteknik Ab | Method of sequencing DNA |
| EP2319854B1 (en) | 1997-01-08 | 2016-11-30 | Sigma-Aldrich Co. LLC | Bioconjugation Of Macromolecules |
| US5837860A (en) | 1997-03-05 | 1998-11-17 | Molecular Tool, Inc. | Covalent attachment of nucleic acid molecules onto solid-phases via disulfide bonds |
| US6023540A (en) | 1997-03-14 | 2000-02-08 | Trustees Of Tufts College | Fiber optic sensor with encoded microspheres |
| US6327410B1 (en) | 1997-03-14 | 2001-12-04 | The Trustees Of Tufts College | Target analyte sensors utilizing Microspheres |
| EP2327797B1 (en) | 1997-04-01 | 2015-11-25 | Illumina Cambridge Limited | Method of nucleic acid sequencing |
| US6969488B2 (en) | 1998-05-22 | 2005-11-29 | Solexa, Inc. | System and apparatus for sequential processing of analytes |
| US5919626A (en) | 1997-06-06 | 1999-07-06 | Orchid Bio Computer, Inc. | Attachment of unmodified nucleic acids to silanized solid phase surfaces |
| US6054274A (en) | 1997-11-12 | 2000-04-25 | Hewlett-Packard Company | Method of amplifying the signal of target nucleic acid sequence analyte |
| US7427678B2 (en) | 1998-01-08 | 2008-09-23 | Sigma-Aldrich Co. | Method for immobilizing oligonucleotides employing the cycloaddition bioconjugation method |
| US7060431B2 (en) | 1998-06-24 | 2006-06-13 | Illumina, Inc. | Method of making and decoding of array sensors with microspheres |
| US6391937B1 (en) | 1998-11-25 | 2002-05-21 | Motorola, Inc. | Polyacrylamide hydrogels and hydrogel arrays made from polyacrylamide reactive prepolymers |
| US6355431B1 (en) | 1999-04-20 | 2002-03-12 | Illumina, Inc. | Detection of nucleic acid amplification reactions using bead arrays |
| DE60040741D1 (de) | 1999-04-20 | 2008-12-18 | Illumina Inc | Detektion von nukleinsäurereaktionen auf kügelchen-arrays |
| US6534266B1 (en) | 1999-04-22 | 2003-03-18 | Albert Einstein College Of Medicine Of Yeshiva University | Assay of transcription sites by multi-fluor fish |
| US7244559B2 (en) | 1999-09-16 | 2007-07-17 | 454 Life Sciences Corporation | Method of sequencing a nucleic acid |
| US6274320B1 (en) | 1999-09-16 | 2001-08-14 | Curagen Corporation | Method of sequencing a nucleic acid |
| US6770441B2 (en) | 2000-02-10 | 2004-08-03 | Illumina, Inc. | Array compositions and methods of making same |
| US6368801B1 (en) | 2000-04-12 | 2002-04-09 | Molecular Staging, Inc. | Detection and amplification of RNA using target-mediated ligation of DNA by RNA ligase |
| US7001792B2 (en) | 2000-04-24 | 2006-02-21 | Eagle Research & Development, Llc | Ultra-fast nucleic acid sequencing device and a method for making and using the same |
| US6291187B1 (en) | 2000-05-12 | 2001-09-18 | Molecular Staging, Inc. | Poly-primed amplification of nucleic acid sequences |
| US6511809B2 (en) | 2000-06-13 | 2003-01-28 | E. I. Du Pont De Nemours And Company | Method for the detection of an analyte by means of a nucleic acid reporter |
| US6323009B1 (en) | 2000-06-28 | 2001-11-27 | Molecular Staging, Inc. | Multiply-primed amplification of nucleic acid sequences |
| GB0018120D0 (en) | 2000-07-24 | 2000-09-13 | Fermentas Ab | Nuclease |
| US7473767B2 (en) | 2001-07-03 | 2009-01-06 | The Institute For Systems Biology | Methods for detection and quantification of analytes in complex mixtures |
| US7057026B2 (en) | 2001-12-04 | 2006-06-06 | Solexa Limited | Labelled nucleotides |
| US7375234B2 (en) | 2002-05-30 | 2008-05-20 | The Scripps Research Institute | Copper-catalysed ligation of azides and acetylenes |
| AU2003259350A1 (en) | 2002-08-23 | 2004-03-11 | Solexa Limited | Modified nucleotides for polynucleotide sequencing |
| CN1791682B (zh) | 2003-02-26 | 2013-05-22 | 凯利达基因组股份有限公司 | 通过杂交进行的随机阵列dna分析 |
| WO2005047543A2 (en) | 2003-06-10 | 2005-05-26 | Applera Corporation | Ligation assay |
| GB0321306D0 (en) | 2003-09-11 | 2003-10-15 | Solexa Ltd | Modified polymerases for improved incorporation of nucleotide analogues |
| US7259258B2 (en) | 2003-12-17 | 2007-08-21 | Illumina, Inc. | Methods of attaching biological compounds to solid supports using triazine |
| EP2789383B1 (en) | 2004-01-07 | 2023-05-03 | Illumina Cambridge Limited | Molecular arrays |
| WO2006064199A1 (en) | 2004-12-13 | 2006-06-22 | Solexa Limited | Improved method of nucleotide detection |
| GB0427236D0 (en) | 2004-12-13 | 2005-01-12 | Solexa Ltd | Improved method of nucleotide detection |
| EP2237189B1 (en) | 2005-01-27 | 2018-08-01 | Cambridge Research & Instrumentation, Inc. | Classifying image features |
| EP2857523A1 (en) | 2005-02-01 | 2015-04-08 | Applied Biosystems, LLC | Method for identifying a sequence in a polynucleotide |
| US7727721B2 (en) | 2005-03-08 | 2010-06-01 | California Institute Of Technology | Hybridization chain reaction amplification for in situ imaging |
| US7989166B2 (en) | 2005-04-12 | 2011-08-02 | In Situ Rcp A/S | Circle probes and their use in the identification of biomolecules |
| JP4990886B2 (ja) | 2005-05-10 | 2012-08-01 | ソレックサ リミテッド | 改良ポリメラーゼ |
| EP3042963A1 (en) | 2005-06-20 | 2016-07-13 | Advanced Cell Diagnostics, Inc. | Methods of detecting nucleic acids in individual cells and of identifying rare cells from large heterogeneous cell populations |
| GB0514936D0 (en) | 2005-07-20 | 2005-08-24 | Solexa Ltd | Preparation of templates for nucleic acid sequencing |
| WO2007120208A2 (en) | 2005-11-14 | 2007-10-25 | President And Fellows Of Harvard College | Nanogrid rolling circle dna sequencing |
| CA2635215C (en) | 2005-12-23 | 2016-08-30 | Nanostring Technologies, Inc. | Compositions comprising oriented, immobilized macromolecules and methods for their preparation |
| WO2007076129A2 (en) | 2005-12-23 | 2007-07-05 | Nanostring Technologies, Inc. | Nanoreporters and methods of manufacturing and use thereof |
| WO2007092538A2 (en) | 2006-02-07 | 2007-08-16 | President And Fellows Of Harvard College | Methods for making nucleotide probes for sequencing and synthesis |
| GB0605584D0 (en) | 2006-03-20 | 2006-04-26 | Olink Ab | Method for analyte detection using proximity probes |
| EP2030011B1 (en) | 2006-05-22 | 2017-02-01 | Nanostring Technologies, Inc. | Systems and methods for analyzing nanoreporters |
| US9201063B2 (en) | 2006-11-16 | 2015-12-01 | General Electric Company | Sequential analysis of biological samples |
| US7655898B2 (en) | 2006-11-30 | 2010-02-02 | Cambridge Research & Instrumentation, Inc. | Optical filter assembly with selectable bandwidth and rejection |
| EP2677309B9 (en) | 2006-12-14 | 2014-11-19 | Life Technologies Corporation | Methods for sequencing a nucleic acid using large scale FET arrays, configured to measure a limited pH range |
| US8262900B2 (en) | 2006-12-14 | 2012-09-11 | Life Technologies Corporation | Methods and apparatus for measuring analytes using large scale FET arrays |
| US8349167B2 (en) | 2006-12-14 | 2013-01-08 | Life Technologies Corporation | Methods and apparatus for detecting molecular interactions using FET arrays |
| EP2155899A2 (en) | 2007-04-10 | 2010-02-24 | Nanostring Technologies, Inc. | Methods and computer systems for identifying target-specific sequences for use in nanoreporters |
| WO2008151127A1 (en) | 2007-06-04 | 2008-12-11 | President And Fellows Of Harvard College | Compounds and methods for chemical ligation |
| US7534991B2 (en) | 2007-07-10 | 2009-05-19 | Cambridge Research & Instrumentation, Inc. | Athermalized birefringent filter apparatus and method |
| EP2051051B1 (en) | 2007-10-16 | 2020-06-03 | Cambridge Research & Instrumentation, Inc. | Spectral imaging system with dynamic optical correction |
| US8199999B2 (en) | 2008-06-17 | 2012-06-12 | Cambridge Research & Instrumentation, Inc. | Image classifier training |
| JP5836803B2 (ja) | 2008-08-14 | 2015-12-24 | ナノストリング テクノロジーズ, インコーポレイテッド | 安定したナノレポーター |
| US20100055733A1 (en) | 2008-09-04 | 2010-03-04 | Lutolf Matthias P | Manufacture and uses of reactive microcontact printing of biomolecules on soft hydrogels |
| US20100137143A1 (en) | 2008-10-22 | 2010-06-03 | Ion Torrent Systems Incorporated | Methods and apparatus for measuring analytes |
| US9714937B2 (en) | 2009-10-13 | 2017-07-25 | Nanostring Technologies, Inc. | Protein detection via nanoreporters |
| EP2529030B1 (en) | 2010-01-29 | 2019-03-13 | Advanced Cell Diagnostics, Inc. | Methods of in situ detection of nucleic acids |
| ES2647390T3 (es) | 2010-02-11 | 2017-12-21 | Nanostring Technologies, Inc | Composiciones y métodos para la detección preferencial de ARN pequeños mediante hibridación con puente y ligación |
| US10267808B2 (en) | 2010-03-08 | 2019-04-23 | California Institute Of Technology | Molecular indicia of cellular constituents and resolving the same by super-resolution technologies in single cells |
| GB201004292D0 (en) * | 2010-03-15 | 2010-04-28 | Olink Ab | Assay for localised detection of analytes |
| KR101866401B1 (ko) | 2010-04-05 | 2018-06-11 | 프로그노시스 바이오사이언스, 인코포레이티드 | 공간적으로 엔코딩된 생물학적 검정 |
| US8462981B2 (en) | 2010-04-07 | 2013-06-11 | Cambridge Research & Instrumentation, Inc. | Spectral unmixing for visualization of samples |
| JP5832537B2 (ja) | 2010-08-05 | 2015-12-16 | ケンブリッジ・リサーチ・アンド・インストルメンテーション・インコーポレーテッド | 試料の強調視覚評価 |
| WO2012049316A1 (en) | 2010-10-15 | 2012-04-19 | Olink Ab | Dynamic range methods |
| ES2562821T3 (es) | 2010-10-21 | 2016-03-08 | Advanced Cell Diagnostics, Inc. | Método ultrasensible para la detección in situ de ácidos nucleicos |
| EP2633081B1 (en) | 2010-10-29 | 2017-01-11 | Olink Bioscience AB | Proximity ligation technology for western blot applications |
| US8951781B2 (en) | 2011-01-10 | 2015-02-10 | Illumina, Inc. | Systems, methods, and apparatuses to image a sample for biological or chemical analysis |
| JP5881746B2 (ja) | 2011-02-15 | 2016-03-09 | ライカ バイオシステムズ ニューキャッスル リミテッド | mRNAの局在インサイチュー検出のための方法 |
| GB201106254D0 (en) | 2011-04-13 | 2011-05-25 | Frisen Jonas | Method and product |
| US8946389B2 (en) | 2011-04-25 | 2015-02-03 | University Of Washington | Compositions and methods for multiplex biomarker profiling |
| GB201107863D0 (en) | 2011-05-11 | 2011-06-22 | Olink Ab | Method and product |
| GB201108678D0 (en) | 2011-05-24 | 2011-07-06 | Olink Ab | Multiplexed proximity ligation assay |
| US9453258B2 (en) | 2011-09-23 | 2016-09-27 | Illumina, Inc. | Methods and compositions for nucleic acid sequencing |
| EP2766498B1 (en) | 2011-10-14 | 2019-06-19 | President and Fellows of Harvard College | Sequencing by structure assembly |
| EP2794928B1 (en) | 2011-12-22 | 2019-02-20 | President and Fellows of Harvard College | Compositions and methods for analyte detection |
| ES2991004T3 (es) | 2011-12-22 | 2024-12-02 | Harvard College | Métodos para la detección de analitos |
| JP6159391B2 (ja) | 2012-04-03 | 2017-07-05 | イラミーナ インコーポレーテッド | 核酸シークエンシングに有用な統合化した読取りヘッド及び流体カートリッジ |
| CA2874492C (en) | 2012-05-22 | 2021-10-19 | British Columbia Cancer Agency Branch | Nano46 genes and methods to predict breast cancer outcome |
| US9914967B2 (en) | 2012-06-05 | 2018-03-13 | President And Fellows Of Harvard College | Spatial sequencing of nucleic acids using DNA origami probes |
| IN2014DN09073A (enExample) | 2012-08-09 | 2015-05-22 | Univ Leland Stanford Junior | |
| US20140378345A1 (en) | 2012-08-14 | 2014-12-25 | 10X Technologies, Inc. | Compositions and methods for sample processing |
| EP2888576B1 (en) | 2012-08-21 | 2023-10-04 | Akoya Biosciences, Inc. | Visualization and measurement of cell compartments |
| US9783841B2 (en) | 2012-10-04 | 2017-10-10 | The Board Of Trustees Of The Leland Stanford Junior University | Detection of target nucleic acids in a cellular sample |
| EP3901280B1 (en) * | 2012-10-17 | 2025-03-12 | 10x Genomics Sweden AB | Methods and product for optimising localised or spatial detection of gene expression in a tissue sample |
| US10174366B2 (en) | 2012-11-14 | 2019-01-08 | Olink Bioscience Ab | Localised RCA-based amplification method |
| EP2925822A4 (en) | 2012-11-27 | 2016-10-12 | Univ Tufts | BIOPOLYMER-BASED INKS AND USE THEREOF |
| EP2943761B1 (en) | 2013-01-10 | 2023-11-29 | Akoya Biosciences, Inc. | Multispectral imaging system and methods |
| WO2014110025A1 (en) | 2013-01-10 | 2014-07-17 | Caliper Life Sciences, Inc. | Whole slide multispectral imaging systems and methods |
| US9512422B2 (en) | 2013-02-26 | 2016-12-06 | Illumina, Inc. | Gel patterned surfaces |
| US10138509B2 (en) | 2013-03-12 | 2018-11-27 | President And Fellows Of Harvard College | Method for generating a three-dimensional nucleic acid containing matrix |
| US9273349B2 (en) | 2013-03-14 | 2016-03-01 | Affymetrix, Inc. | Detection of nucleic acids |
| US11441196B2 (en) | 2013-03-15 | 2022-09-13 | The Broad Institute, Inc. | Ribosomal ribonucleic acid hybridization for organism identification |
| US10510435B2 (en) | 2013-04-30 | 2019-12-17 | California Institute Of Technology | Error correction of multiplex imaging analysis by sequential hybridization |
| KR102368591B1 (ko) | 2013-04-30 | 2022-02-25 | 캘리포니아 인스티튜트 오브 테크놀로지 | 순차적 하이브리드화 바코딩에 의한 분자의 멀티플렉스 표지화 |
| US20160369329A1 (en) | 2013-04-30 | 2016-12-22 | California Institute Of Technology | Multiplex labeling of molecules by sequential hybridization barcoding using probes with cleavable linkers |
| CN111500680B (zh) | 2013-06-25 | 2024-04-16 | 普罗格诺西斯生物科学公司 | 检测样品中生物靶标的空间分布的方法和系统 |
| JP6546177B2 (ja) | 2013-09-13 | 2019-07-17 | ザ ボード オブ トラスティーズ オブ ザ レランド スタンフォード ジュニア ユニバーシティー | 質量タグ及び二次イオン質量分析計を用いた組織の多重化イメージング |
| US10794802B2 (en) | 2013-09-20 | 2020-10-06 | California Institute Of Technology | Whole-body tissue stabilization and selective extractions via tissue-hydrogel hybrids for high resolution intact circuit mapping and phenotyping |
| US9778154B2 (en) | 2013-09-20 | 2017-10-03 | California Institute Of Technology | Methods for phenotyping of intact whole tissues |
| GB201401885D0 (en) | 2014-02-04 | 2014-03-19 | Olink Ab | Proximity assay with detection based on hybridisation chain reaction (HCR) |
| EP3108218A4 (en) | 2014-02-21 | 2017-11-15 | Massachusetts Institute Of Technology | Expansion microscopy |
| US10858698B2 (en) | 2014-03-25 | 2020-12-08 | President And Fellows Of Harvard College | Barcoded protein array for multiplex single-molecule interaction profiling |
| CN106460069B (zh) | 2014-04-18 | 2021-02-12 | 威廉马歇莱思大学 | 用于富集含稀有等位基因的物质的核酸分子的竞争性组合物 |
| WO2015184124A1 (en) | 2014-05-30 | 2015-12-03 | The Board Of Trustees Of The Leland Stanford Junior University | Methods and devices for imaging large intact tissue samples |
| US10829814B2 (en) | 2014-06-19 | 2020-11-10 | Illumina, Inc. | Methods and compositions for single cell genomics |
| US9909167B2 (en) | 2014-06-23 | 2018-03-06 | The Board Of Trustees Of The Leland Stanford Junior University | On-slide staining by primer extension |
| EP4574974A3 (en) | 2014-06-26 | 2025-10-08 | 10x Genomics, Inc. | Methods of analyzing nucleic acids from individual cells or cell populations |
| EP3167278B1 (en) | 2014-07-09 | 2019-06-26 | Caliper Life Sciences, Inc. | Pure spectrum extraction from biological samples |
| WO2016007839A1 (en) | 2014-07-11 | 2016-01-14 | President And Fellows Of Harvard College | Methods for high-throughput labelling and detection of biological features in situ using microscopy |
| EP3169810B1 (en) | 2014-07-17 | 2024-09-04 | California Institute of Technology | Multiplex analysis of molecules in single cells by image correlation |
| EP4273263A3 (en) | 2014-07-30 | 2024-01-31 | President and Fellows of Harvard College | Systems and methods for determining nucleic acids |
| WO2016028843A2 (en) | 2014-08-19 | 2016-02-25 | President And Fellows Of Harvard College | Rna-guided systems for probing and mapping of nucleic acids |
| US11091810B2 (en) | 2015-01-27 | 2021-08-17 | BioSpyder Technologies, Inc. | Focal gene expression profiling of stained FFPE tissues with spatial correlation to morphology |
| US20160108458A1 (en) | 2014-10-06 | 2016-04-21 | The Board Of Trustees Of The Leland Stanford Junior University | Multiplexed detection and quantification of nucleic acids in single-cells |
| AU2015349870B2 (en) | 2014-11-21 | 2019-09-26 | Bruker Spatial Biology, Inc. | Enzyme- and amplification-free sequencing |
| ES2756399T3 (es) | 2014-11-24 | 2020-04-27 | Nanostring Technologies Inc | Métodos y aparatos para la purificación y formación de imágenes de genes |
| WO2016138496A1 (en) | 2015-02-27 | 2016-09-01 | Cellular Research, Inc. | Spatially addressable molecular barcoding |
| CN107406888A (zh) | 2015-03-30 | 2017-11-28 | 赛卢拉研究公司 | 用于组合条形编码的方法和组合物 |
| EP4321627A3 (en) | 2015-04-10 | 2024-04-17 | 10x Genomics Sweden AB | Spatially distinguished, multiplex nucleic acid analysis of biological specimens |
| US10059990B2 (en) | 2015-04-14 | 2018-08-28 | Massachusetts Institute Of Technology | In situ nucleic acid sequencing of expanded biological samples |
| US11408890B2 (en) | 2015-04-14 | 2022-08-09 | Massachusetts Institute Of Technology | Iterative expansion microscopy |
| US10526649B2 (en) | 2015-04-14 | 2020-01-07 | Massachusetts Institute Of Technology | Augmenting in situ nucleic acid sequencing of expanded biological samples with in vitro sequence information |
| CN107709574B (zh) | 2015-04-14 | 2021-10-01 | 皇家飞利浦有限公司 | 生物组织样品的分子概况的空间作图 |
| US20180057873A1 (en) | 2015-04-17 | 2018-03-01 | Centrillion Technology Holdings Corporation | Methods for performing spatial profiling of biological materials |
| CN114350752B (zh) | 2015-07-17 | 2025-02-25 | 布鲁克空间生物学公司 | 在横切面组织的用户定义区域中的基因表达的同时定量 |
| US10501777B2 (en) | 2015-07-17 | 2019-12-10 | Nanostring Technologies, Inc. | Simultaneous quantification of a plurality of proteins in a user-defined region of a cross-sectioned tissue |
| WO2017019481A1 (en) | 2015-07-24 | 2017-02-02 | The Johns Hopkins University | Compositions and methods of rna analysis |
| US10913975B2 (en) | 2015-07-27 | 2021-02-09 | Illumina, Inc. | Spatial mapping of nucleic acid sequence information |
| EP3332029B1 (en) | 2015-08-07 | 2021-10-06 | Massachusetts Institute of Technology | Nanoscale imaging of proteins and nucleic acids via expansion microscopy |
| WO2017027368A1 (en) | 2015-08-07 | 2017-02-16 | Massachusetts Institute Of Technology | Protein retention expansion microscopy |
| US11214661B2 (en) | 2015-09-17 | 2022-01-04 | Massachusetts Institute Of Technology | Three-dimensional nanofabrication by patterning of hydrogels |
| EP3913068B1 (en) | 2015-10-12 | 2023-08-02 | Advanced Cell Diagnostics, Inc. | In situ detection of nucleotide variants in high noise samples, and compositions and methods related thereto |
| US11111487B2 (en) | 2015-10-28 | 2021-09-07 | Silicon Valley Scientific, Inc. | Method and apparatus for encoding cellular spatial position information |
| WO2017075275A1 (en) | 2015-10-29 | 2017-05-04 | The Board Of Trustees Of The Leland Stanford Junior University | Methods and systems for imaging a biological sample |
| EP3371691B1 (en) | 2015-11-03 | 2026-03-11 | President and Fellows of Harvard College | Systems and methods for processing spatially related sequence data received from a sequencing device |
| CN108474022A (zh) | 2015-11-03 | 2018-08-31 | 哈佛学院董事及会员团体 | 用于包含三维核酸的基质容积成像的设备和方法 |
| US11254974B2 (en) | 2016-02-10 | 2022-02-22 | The Board Of Trustees Of The Leland Stanford Junior University | RNA fixation and detection in clarity-based hydrogel tissue |
| WO2017143155A2 (en) | 2016-02-18 | 2017-08-24 | President And Fellows Of Harvard College | Multiplex alteration of cells using a pooled nucleic acid library and analysis thereof |
| US11360025B2 (en) | 2016-02-22 | 2022-06-14 | Miltenyi Biotec B.V. & Co. KG | Automated analysis tool for biological specimens |
| US20170241911A1 (en) | 2016-02-22 | 2017-08-24 | Miltenyi Biotec Gmbh | Automated analysis tool for biological specimens |
| EP4015647B1 (en) | 2016-02-26 | 2023-08-30 | The Board of Trustees of the Leland Stanford Junior University | Multiplexed single molecule rna visualization with a two-probe proximity ligation system |
| US20170253918A1 (en) | 2016-03-01 | 2017-09-07 | Expansion Technologies | Combining protein barcoding with expansion microscopy for in-situ, spatially-resolved proteomics |
| US10844426B2 (en) | 2016-03-17 | 2020-11-24 | President And Fellows Of Harvard College | Methods for detecting and identifying genomic nucleic acids |
| GB2565461B (en) | 2016-03-31 | 2022-04-13 | Harvard College | Methods and compositions for the single tube preparation of sequencing libraries using Cas9 |
| AU2017257624B2 (en) | 2016-04-25 | 2023-05-25 | President And Fellows Of Harvard College | Hybridization chain reaction methods for in situ molecular detection |
| US20180052081A1 (en) | 2016-05-11 | 2018-02-22 | Expansion Technologies | Combining modified antibodies with expansion microscopy for in-situ, spatially-resolved proteomics |
| CN116200462B (zh) | 2016-05-16 | 2025-10-03 | 布鲁克空间生物学公司 | 用于检测样品中目标核酸的方法 |
| US10495554B2 (en) | 2016-05-25 | 2019-12-03 | The Board Of Trustees Of The Leland Stanford Junior University | Method and system for imaging and analysis of a biological specimen |
| US11352667B2 (en) | 2016-06-21 | 2022-06-07 | 10X Genomics, Inc. | Nucleic acid sequencing |
| ES2908919T3 (es) | 2016-07-05 | 2022-05-04 | California Inst Of Techn | Reacción en cadena de hibridación de iniciador fraccionario |
| EP3490616B1 (en) | 2016-07-27 | 2022-09-28 | The Board of Trustees of the Leland Stanford Junior University | Highly-multiplexed fluorescent imaging |
| CA3032649A1 (en) | 2016-08-01 | 2018-02-08 | California Institute Of Technology | Sequential probing of molecular targets based on pseudo-color barcodes with embedded error correction mechanism |
| US12421540B2 (en) | 2016-08-01 | 2025-09-23 | California Institute Of Technology | Sequential probing of molecular targets based on pseudo-color barcodes with embedded error correction mechanism |
| AU2017321492A1 (en) | 2016-08-30 | 2019-03-14 | California Institute Of Technology | Immunohistochemistry via hybridization chain reaction |
| EP4582805A3 (en) | 2016-08-31 | 2025-10-08 | President And Fellows Of Harvard College | Methods of generating libraries of nucleic acid sequences for detection via fluorescent in situ sequencing |
| GB2569252A (en) | 2016-08-31 | 2019-06-12 | Harvard College | Methods of combining the detection of biomolecules into a single assay using fluorescent in situ sequencing |
| CN110352252B (zh) | 2016-09-22 | 2024-06-25 | 威廉马歇莱思大学 | 用于复杂序列捕获和分析的分子杂交探针 |
| CN110140175A (zh) | 2016-11-08 | 2019-08-16 | 哈佛学院院长及董事 | 基质印迹和清除 |
| US20190276881A1 (en) | 2016-11-08 | 2019-09-12 | President And Fellows Of Harvard College | Multiplexed imaging using merfish, expansion microscopy, and related technologies |
| GB201619458D0 (en) | 2016-11-17 | 2017-01-04 | Spatial Transcriptomics Ab | Method for spatial tagging and analysing nucleic acids in a biological specimen |
| AU2017361521B2 (en) | 2016-11-21 | 2020-08-27 | Bruker Spatial Biology, Inc. | Chemical compositions and methods of using same |
| US10656144B2 (en) | 2016-12-02 | 2020-05-19 | The Charlotte Mecklenburg Hospital Authority | Immune profiling and minimal residue disease following stem cell transplantation in multiple myeloma |
| CN110050071A (zh) | 2016-12-09 | 2019-07-23 | 乌尔蒂维尤股份有限公司 | 用于使用标记的核酸成像剂进行多路复用成像的改进方法 |
| US20190177800A1 (en) | 2017-12-08 | 2019-06-13 | 10X Genomics, Inc. | Methods and compositions for labeling cells |
| US10550429B2 (en) | 2016-12-22 | 2020-02-04 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US11492661B2 (en) | 2017-01-10 | 2022-11-08 | President And Fellows Of Harvard College | Multiplexed signal amplification |
| US10995361B2 (en) | 2017-01-23 | 2021-05-04 | Massachusetts Institute Of Technology | Multiplexed signal amplified FISH via splinted ligation amplification and sequencing |
| WO2018175779A1 (en) | 2017-03-22 | 2018-09-27 | The Board Of Trustees Of The Leland Stanford Junior University | Molecular profiling using proximity ligation-in situ hybridization |
| WO2018217905A1 (en) | 2017-05-23 | 2018-11-29 | Rutgers, The State University Of New Jersey | Target mediated in situ signal amplification with dual interacting hairpin probes |
| US11180804B2 (en) | 2017-07-25 | 2021-11-23 | Massachusetts Institute Of Technology | In situ ATAC sequencing |
| WO2019068880A1 (en) | 2017-10-06 | 2019-04-11 | Cartana Ab | RNA MATRIX LIGATION |
| US11753676B2 (en) | 2017-10-11 | 2023-09-12 | Expansion Technologies | Multiplexed in situ hybridization of tissue sections for spatially resolved transcriptomics with expansion microscopy |
| US11479811B2 (en) | 2017-11-21 | 2022-10-25 | Expansion Technologies | Expansion microscopy compatible and multiplexed in situ hybridization of formalin fixed paraffin embedded tissue sections for spatially resolved transcriptomics |
| CN111699268B (zh) | 2017-12-08 | 2024-04-26 | 加州理工学院 | 通过用探针的快速切换和再杂交进行顺序杂交编条形码来多重标记分子 |
| EP3752832A1 (en) | 2018-02-12 | 2020-12-23 | 10X Genomics, Inc. | Methods characterizing multiple analytes from individual cells or cell populations |
| JP7372927B6 (ja) | 2018-02-12 | 2023-11-27 | ナノストリング テクノロジーズ,インコーポレイティド | 遺伝子およびタンパク質の発現を検出する生体分子プローブおよびその検出方法 |
| CN112074610A (zh) | 2018-02-22 | 2020-12-11 | 10X基因组学有限公司 | 接合介导的核酸分析 |
| EP3775271B1 (en) | 2018-04-06 | 2025-03-12 | 10X Genomics, Inc. | Systems and methods for quality control in single cell processing |
| CN112243463B (zh) * | 2018-04-09 | 2024-12-13 | 小利兰·斯坦福大学托管委员会 | 原位基因测序方法 |
| CN120629082A (zh) | 2018-05-03 | 2025-09-12 | 阿科亚生物科学股份有限公司 | 多光谱样本成像 |
| CN112770776B (zh) | 2018-07-30 | 2025-08-19 | 瑞德库尔有限责任公司 | 用于样品处理或分析的方法和系统 |
| WO2020047005A1 (en) | 2018-08-28 | 2020-03-05 | 10X Genomics, Inc. | Resolving spatial arrays |
| CN113767175B (zh) | 2018-08-28 | 2025-07-25 | 10X基因组学股份有限公司 | 增加空间阵列分辨率 |
| EP3847274A4 (en) | 2018-09-05 | 2022-06-08 | Readcoor, LLC | METHODS AND SYSTEMS FOR THE ANALYSIS OF THERAPEUTIC AGENT |
| WO2020076976A1 (en) | 2018-10-10 | 2020-04-16 | Readcoor, Inc. | Three-dimensional spatial molecular indexing |
| EP3864173B1 (en) | 2018-10-10 | 2025-08-27 | 10x Genomics, Inc. | Surface capture of target nucleic acid molecules |
| WO2020082087A1 (en) | 2018-10-19 | 2020-04-23 | Akoya Biosciences, Inc. | Detection of co-occurring receptor-coding nucleic acid segments |
| GB201818742D0 (en) | 2018-11-16 | 2019-01-02 | Cartana Ab | Method for detection of RNA |
| JP7332694B2 (ja) | 2018-12-04 | 2023-08-23 | エフ. ホフマン-ラ ロシュ アーゲー | 細胞標的の空間指向性量子バーコード化 |
| EP3894585A2 (en) | 2018-12-10 | 2021-10-20 | 10X Genomics, Inc. | Generating capture probes for spatial analysis |
| CA3122569A1 (en) | 2018-12-13 | 2020-06-18 | President And Fellows Of Harvard College | Amplification methods and systems for merfish and other applications |
| WO2020142490A1 (en) | 2018-12-31 | 2020-07-09 | Expansion Technologies | Expansion microscopy compatible anchorable h&e staining for histopathology |
| US12529092B2 (en) * | 2019-01-28 | 2026-01-20 | The Broad Institute, Inc. | In-situ spatial transcriptomics |
| CN119220641A (zh) | 2019-05-31 | 2024-12-31 | 10X基因组学有限公司 | 检测目标核酸分子的方法 |
| US11994452B2 (en) | 2019-06-14 | 2024-05-28 | Akoya Biosciences, Inc. | Multiplexed tissue imaging |
| EP3754028A1 (en) | 2019-06-18 | 2020-12-23 | Apollo Life Sciences GmbH | Method of signal encoding of analytes in a sample |
| GB201919029D0 (en) | 2019-12-20 | 2020-02-05 | Cartana Ab | Method of detecting an analyte |
| GB201919032D0 (en) | 2019-12-20 | 2020-02-05 | Cartana Ab | Method of detecting an analyte |
| WO2021155063A1 (en) | 2020-01-29 | 2021-08-05 | Readcoor, Llc | Compositions and methods for analyte detection |
| US20210238662A1 (en) | 2020-02-03 | 2021-08-05 | 10X Genomics, Inc. | Probes and methods of using same |
| US12110548B2 (en) | 2020-02-03 | 2024-10-08 | 10X Genomics, Inc. | Bi-directional in situ analysis |
| WO2021167986A1 (en) | 2020-02-17 | 2021-08-26 | 10X Genomics, Inc. | In situ analysis of chromatin interaction |
| EP4107285B1 (en) | 2020-02-21 | 2024-10-09 | 10X Genomics, Inc. | Methods and compositions for integrated in situ spatial assay |
| WO2021168326A1 (en) | 2020-02-21 | 2021-08-26 | Readcoor, Llc | Compositions, methods and systems for sample processing |
| US12188085B2 (en) | 2020-03-05 | 2025-01-07 | 10X Genomics, Inc. | Three-dimensional spatial transcriptomics with sequencing readout |
| US20210340621A1 (en) | 2020-04-22 | 2021-11-04 | Readcoor, Llc | Methods and systems for sequencing |
| US12209273B2 (en) | 2020-06-12 | 2025-01-28 | 10X Genomics, Inc. | Nucleic acid assays using click chemistry bioconjugation |
| US20210388424A1 (en) | 2020-06-12 | 2021-12-16 | 10X Genomics, Inc. | Methods for analyzing target nucleic acids and related compositions |
| US12297499B2 (en) | 2020-08-17 | 2025-05-13 | 10X Genomics, Inc. | Multicomponent nucleic acid probes for sample analysis |
| US20220049303A1 (en) | 2020-08-17 | 2022-02-17 | Readcoor, Llc | Methods and systems for spatial mapping of genetic variants |
| WO2022060889A2 (en) | 2020-09-16 | 2022-03-24 | 10X Genomics, Inc. | Methods and systems for barcode error correction |
| US12071667B2 (en) | 2020-11-04 | 2024-08-27 | 10X Genomics, Inc. | Sequence analysis using meta-stable nucleic acid molecules |
| EP4012046A1 (en) | 2020-12-11 | 2022-06-15 | 10X Genomics, Inc. | Methods and compositions for multimodal in situ analysis |
| CN116848263A (zh) | 2020-12-23 | 2023-10-03 | 10X基因组学有限公司 | 用于分析物检测的方法和组合物 |
| US12060603B2 (en) | 2021-01-19 | 2024-08-13 | 10X Genomics, Inc. | Methods for internally controlled in situ assays using padlock probes |
-
2021
- 2021-02-19 EP EP21711699.5A patent/EP4107285B1/en active Active
- 2021-02-19 ES ES21711699T patent/ES2993641T3/es active Active
- 2021-02-19 WO PCT/US2021/018826 patent/WO2021168287A1/en not_active Ceased
- 2021-02-19 AU AU2021225020A patent/AU2021225020A1/en not_active Abandoned
- 2021-02-19 US US17/180,325 patent/US12173360B2/en active Active
- 2021-02-19 CA CA3168202A patent/CA3168202A1/en active Pending
- 2021-02-19 EP EP24197343.7A patent/EP4484571A3/en active Pending
- 2021-02-19 CN CN202180029933.1A patent/CN115698324A/zh active Pending
- 2021-02-19 JP JP2022550866A patent/JP2023514749A/ja active Pending
-
2024
- 2024-11-11 US US18/943,775 patent/US20250137040A1/en active Pending
Non-Patent Citations (3)
| Title |
|---|
| BIOESSAYS, vol. 42, no. 10, JPN6025017486, May 2020 (2020-05-01), pages 1900221 - 1, ISSN: 0005721225 * |
| NAT. PROTOC., vol. 13, no. 11, JPN6025017484, 2018, pages 2501 - 2534, ISSN: 0005585333 * |
| NUCLEIC ACIDS RES., vol. 48, no. 19, JPN6025017485, 29 September 2020 (2020-09-29), pages 112 - 1, ISSN: 0005585332 * |
Also Published As
| Publication number | Publication date |
|---|---|
| US12173360B2 (en) | 2024-12-24 |
| CA3168202A1 (en) | 2021-08-26 |
| US20250137040A1 (en) | 2025-05-01 |
| EP4107285B1 (en) | 2024-10-09 |
| EP4484571A2 (en) | 2025-01-01 |
| CN115698324A (zh) | 2023-02-03 |
| AU2021225020A1 (en) | 2022-08-18 |
| EP4107285A1 (en) | 2022-12-28 |
| WO2021168287A1 (en) | 2021-08-26 |
| EP4107285C0 (en) | 2024-10-09 |
| ES2993641T3 (en) | 2025-01-03 |
| US20210262018A1 (en) | 2021-08-26 |
| EP4484571A3 (en) | 2025-03-19 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US20250137040A1 (en) | Methods and compositions for integrated in situ spatial assay | |
| US11753675B2 (en) | Generating capture probes for spatial analysis | |
| US11926867B2 (en) | Generating capture probes for spatial analysis | |
| US11808769B2 (en) | Spatially-tagged analyte capture agents for analyte multiplexing | |
| US20250154569A1 (en) | Generating capture probes for spatial analysis | |
| US20250129421A1 (en) | Method for transposase-mediated spatial tagging and analyzing genomic dna in a biological sample | |
| US11933957B1 (en) | Imaging system hardware | |
| CN113366117B (zh) | 用于生物样品中转座酶介导的空间标记和分析基因组dna的方法 | |
| US20210317524A1 (en) | Resolving spatial arrays | |
| US20210324457A1 (en) | Methods for Generating Spatially Barcoded Arrays | |
| CN114127309A (zh) | 使用空间阵列进行单细胞测序的方法 | |
| US20260002202A1 (en) | Generating capture probes for spatial analysis |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20221020 Free format text: JAPANESE INTERMEDIATE CODE: A821 Effective date: 20220819 |
|
| RD04 | Notification of resignation of power of attorney |
Free format text: JAPANESE INTERMEDIATE CODE: A7424 Effective date: 20230821 |
|
| A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20240216 |
|
| A621 | Written request for application examination |
Free format text: JAPANESE INTERMEDIATE CODE: A621 Effective date: 20240216 |
|
| A977 | Report on retrieval |
Free format text: JAPANESE INTERMEDIATE CODE: A971007 Effective date: 20250313 |
|
| A131 | Notification of reasons for refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A131 Effective date: 20250501 |
|
| A02 | Decision of refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A02 Effective date: 20251031 |