JP2023508465A - 粒子および細胞を処理するためのマイクロ流体カートリッジ - Google Patents

粒子および細胞を処理するためのマイクロ流体カートリッジ Download PDF

Info

Publication number
JP2023508465A
JP2023508465A JP2022539341A JP2022539341A JP2023508465A JP 2023508465 A JP2023508465 A JP 2023508465A JP 2022539341 A JP2022539341 A JP 2022539341A JP 2022539341 A JP2022539341 A JP 2022539341A JP 2023508465 A JP2023508465 A JP 2023508465A
Authority
JP
Japan
Prior art keywords
cells
obstacles
sample
cartridge
microfluidic
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP2022539341A
Other languages
English (en)
Japanese (ja)
Other versions
JP2023508465A5 (https=
Inventor
スケリー，アリソン
ガンディ，クシュルー
ウォード，アンソニー
バーマーディ，ヤスナ
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Curate Biosciences LLC
Original Assignee
GPB Scientific Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by GPB Scientific Inc filed Critical GPB Scientific Inc
Publication of JP2023508465A publication Critical patent/JP2023508465A/ja
Publication of JP2023508465A5 publication Critical patent/JP2023508465A5/ja
Pending legal-status Critical Current

Links

Images

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads or physically stretching molecules
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502707—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the manufacture of the container or its components
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502753—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by bulk separation arrangements on lab-on-a-chip devices, e.g. for filtration or centrifugation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/16—Microfluidic devices; Capillary tubes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/10—Separation or concentration of fermentation products
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/12—Purification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0634—Cells from the blood or the immune system
    • C12N5/0636—T lymphocytes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/04—Exchange or ejection of cartridges, containers or reservoirs
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0631—Purification arrangements, e.g. solid phase extraction [SPE]
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
    • B01L2200/0652—Sorting or classification of particles or molecules
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0848—Specific forms of parts of containers
    • B01L2300/0851—Bottom walls
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0864—Configuration of multiple channels and/or chambers in a single devices comprising only one inlet and multiple receiving wells, e.g. for separation, splitting
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0887—Laminated structure
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/12—Specific details about materials
    • B01L2300/123—Flexible; Elastomeric
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Clinical Laboratory Science (AREA)
  • Hematology (AREA)
  • Dispersion Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Analytical Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Sustainable Development (AREA)
  • Fluid Mechanics (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Cell Biology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Physical Or Chemical Processes And Apparatus (AREA)
  • Micromachines (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
JP2022539341A 2019-12-28 2020-12-23 粒子および細胞を処理するためのマイクロ流体カートリッジ Pending JP2023508465A (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201962954478P 2019-12-28 2019-12-28
US62/954,478 2019-12-28
PCT/US2020/066812 WO2021133897A1 (en) 2019-12-28 2020-12-23 Microfluidic cartridges for processing particles and cells

Publications (2)

Publication Number Publication Date
JP2023508465A true JP2023508465A (ja) 2023-03-02
JP2023508465A5 JP2023508465A5 (https=) 2024-01-04

Family

ID=74184958

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2022539341A Pending JP2023508465A (ja) 2019-12-28 2020-12-23 粒子および細胞を処理するためのマイクロ流体カートリッジ

Country Status (8)

Country Link
US (1) US20230028754A1 (https=)
EP (1) EP4081344A1 (https=)
JP (1) JP2023508465A (https=)
KR (1) KR20220129566A (https=)
CN (1) CN115209996B (https=)
AU (1) AU2020411478A1 (https=)
CA (1) CA3166192A1 (https=)
WO (1) WO2021133897A1 (https=)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2010006174A2 (en) * 2008-07-10 2010-01-14 Reichenbach Steven H Method and apparatus for sorting particles using asymmetrical particle shifting
US20230139871A1 (en) * 2021-11-02 2023-05-04 Terumo Bct, Inc. Systems and Methods For Using Microfluidic Devices With Apheresis Systems
US20240368511A1 (en) * 2022-01-24 2024-11-07 Belement Inc. Microfluidic device for manufacturing bioscaffold and use thereof
US20260097163A1 (en) * 2022-09-22 2026-04-09 Astrin Biosciences, Inc. Pressure balancing across microfluidic devices
TWI889038B (zh) * 2023-04-14 2025-07-01 前瞻優勢生醫股份有限公司 微流體微粒分選器
CN117619731A (zh) * 2023-10-24 2024-03-01 昆明理工大学 一种多级微塑料颗粒分选装置与方法
WO2025151923A1 (en) * 2024-01-17 2025-07-24 Newsouth Innovations Pty Limited Apparatus for production of engineered cells
CN118847237B (zh) * 2024-07-19 2025-05-02 深圳市睿迈生物科技有限公司 一种具有非一致性设计参数的确定性侧向位移芯片及其设计方法

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20060223178A1 (en) * 2005-04-05 2006-10-05 Tom Barber Devices and methods for magnetic enrichment of cells and other particles
US20100006479A1 (en) * 2008-07-10 2010-01-14 Reichenbach Steven H Method and apparatus for sorting particles using asymmetrical particle shifting
WO2016019393A1 (en) * 2014-08-01 2016-02-04 Gpb Scientific, Llc Methods and systems for processing particles
WO2019222049A1 (en) * 2018-05-13 2019-11-21 Gpb Scientific, Llc Combined purification and concentration by deterministic lateral displacement with recirculation product
US20190366342A1 (en) * 2016-10-24 2019-12-05 Gpb Scientific, Llc Deterministic lateral displacement in the preparation of cells and compositions for therapeutic uses

Family Cites Families (48)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5427663A (en) 1993-06-08 1995-06-27 British Technology Group Usa Inc. Microlithographic array for macromolecule and cell fractionation
AU2002251946A1 (en) 2001-02-14 2002-08-28 Science And Technology Corporation @ Unm Nanostructured devices for separation and analysis
US6913697B2 (en) 2001-02-14 2005-07-05 Science & Technology Corporation @ Unm Nanostructured separation and analysis devices for biological membranes
WO2003066191A1 (en) 2002-02-04 2003-08-14 Colorado School Of Mines Laminar flow-based separations of colloidal and cellular particles
EP2359689B1 (en) 2002-09-27 2015-08-26 The General Hospital Corporation Microfluidic device for cell separation and use thereof
EP1585583B1 (en) 2002-10-23 2010-04-07 The Trustees of Princeton University Method for continuous particle separation using obstacle arrays asymmetrically aligned to fields
EP1636564A1 (en) 2003-06-13 2006-03-22 The General Hospital Corporation Microfluidic systems for size based removal of red blood cells and platelets from blood
US20060121624A1 (en) 2004-03-03 2006-06-08 Huang Lotien R Methods and systems for fluid delivery
US20050266433A1 (en) 2004-03-03 2005-12-01 Ravi Kapur Magnetic device for isolation of cells and biomolecules in a microfluidic environment
US20050282293A1 (en) 2004-03-03 2005-12-22 Cosman Maury D System for delivering a diluted solution
US20070026414A1 (en) 2005-07-29 2007-02-01 Martin Fuchs Devices and methods for enrichment and alteration of circulating tumor cells and other particles
US20070026417A1 (en) 2005-07-29 2007-02-01 Martin Fuchs Devices and methods for enrichment and alteration of circulating tumor cells and other particles
US20070026413A1 (en) 2005-07-29 2007-02-01 Mehmet Toner Devices and methods for enrichment and alteration of circulating tumor cells and other particles
EP1874920A4 (en) 2005-04-05 2009-11-04 Cellpoint Diagnostics DEVICES AND METHOD FOR ENRICHING AND CHANGING CIRCULATING TUMOR CELLS AND OTHER PARTICLES
US20070026415A1 (en) 2005-07-29 2007-02-01 Martin Fuchs Devices and methods for enrichment and alteration of circulating tumor cells and other particles
US20070196820A1 (en) 2005-04-05 2007-08-23 Ravi Kapur Devices and methods for enrichment and alteration of cells and other particles
TWI273484B (en) 2005-06-15 2007-02-11 Inventec Corp Method for increasing system performance
US20070059680A1 (en) 2005-09-15 2007-03-15 Ravi Kapur System for cell enrichment
US20070059719A1 (en) 2005-09-15 2007-03-15 Michael Grisham Business methods for prenatal Diagnosis
US20070059774A1 (en) 2005-09-15 2007-03-15 Michael Grisham Kits for Prenatal Testing
US20070059718A1 (en) 2005-09-15 2007-03-15 Mehmet Toner Systems and methods for enrichment of analytes
US20070059781A1 (en) 2005-09-15 2007-03-15 Ravi Kapur System for size based separation and analysis
US7735652B2 (en) 2006-06-01 2010-06-15 The Trustees Of Princeton University Apparatus and method for continuous particle separation
US20080090239A1 (en) 2006-06-14 2008-04-17 Daniel Shoemaker Rare cell analysis using sample splitting and dna tags
US20080113358A1 (en) 2006-07-28 2008-05-15 Ravi Kapur Selection of cells using biomarkers
US7807454B2 (en) * 2006-10-18 2010-10-05 The Regents Of The University Of California Microfluidic magnetophoretic device and methods for using the same
US20100233693A1 (en) * 2007-04-16 2010-09-16 On-O-ity, Inc Methods for diagnosing, prognosing, or theranosing a condition using rare cells
WO2010011934A2 (en) 2008-07-24 2010-01-28 The Trustees Of Princeton University Bump array device having asymmetric gaps for segregation of particles
WO2010129441A2 (en) * 2009-05-04 2010-11-11 Gpb Scientific, Llc Method for separating stem cells from their more differentiated progeny using microfluidic devices
WO2012016136A2 (en) * 2010-07-30 2012-02-02 The General Hospital Corporation Microscale and nanoscale structures for manipulating particles
PH12013501201A1 (en) 2010-12-09 2013-07-29 Univ Pennsylvania Use of chimeric antigen receptor-modified t cells to treat cancer
WO2012094642A2 (en) 2011-01-06 2012-07-12 On-Q-ity Circulating tumor cell capture on a microfluidic chip incorporating both affinity and size
CA3285826A1 (en) 2012-02-22 2026-03-02 The Trustees Of The University Of Pennsylvania Compositions and methods for generating a persisting population of t cells useful for the treatment of cancer
RU2658485C2 (ru) 2012-10-24 2018-06-21 Дзе Юнайтед Стейтс Оф Америка, Эз Репрезентед Бай Дзе Секретари, Департмент Оф Хелс Энд Хьюман Сёрвисез Химерные антигенные рецепторы м971
US11493428B2 (en) * 2013-03-15 2022-11-08 Gpb Scientific, Inc. On-chip microfluidic processing of particles
US20140342375A1 (en) 2013-03-15 2014-11-20 University Of Maryland Microfluidic processing of leukocytes for molecular diagnostic testing
US12590955B2 (en) * 2013-03-15 2026-03-31 Zeon Corporation Methods and systems for processing particles
EP3608022A1 (en) 2013-03-15 2020-02-12 The Trustees of Princeton University Methods and devices for high throughput purification
EP3783098A1 (en) 2013-05-14 2021-02-24 Board Of Regents, The University Of Texas System Human application of engineered chimeric antigen receptor (car) t-cells
WO2015179801A1 (en) 2014-05-23 2015-11-26 University Of Florida Research Foundation, Inc. Car based immunotherapy
JP6754761B2 (ja) 2014-07-11 2020-09-16 セルジーン コーポレイション Ｔリンパ球へのベクター導入効率の改善方法
JP2017529841A (ja) 2014-09-19 2017-10-12 リジェネロン・ファーマシューティカルズ・インコーポレイテッドＲｅｇｅｎｅｒｏｎ Ｐｈａｒｍａｃｅｕｔｉｃａｌｓ， Ｉｎｃ． キメラ抗原受容体
EP3206705A4 (en) 2014-10-16 2018-05-23 Piramal Enterprises Limited Stable injectable composition of bivalirudin and process for its preparation
AU2016274989A1 (en) 2015-06-12 2017-11-02 Immunomedics, Inc. Disease therapy with chimeric antigen receptor (car) constructs and t cells (car-t) or nk cells (car-nk) expressing car constructs
US10976232B2 (en) * 2015-08-24 2021-04-13 Gpb Scientific, Inc. Methods and devices for multi-step cell purification and concentration
CN115655825A (zh) * 2015-09-18 2023-01-31 紫荆实验室股份有限公司 利用表面附着结构的流动池，以及相关的系统和方法
US9719926B2 (en) * 2015-11-16 2017-08-01 International Business Machines Corporation Nanopillar microfluidic devices and methods of use thereof
CN111065399B (zh) 2017-09-01 2023-11-28 Gpb科学有限公司 使用微流体制备治疗活性细胞的方法

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20060223178A1 (en) * 2005-04-05 2006-10-05 Tom Barber Devices and methods for magnetic enrichment of cells and other particles
US20100006479A1 (en) * 2008-07-10 2010-01-14 Reichenbach Steven H Method and apparatus for sorting particles using asymmetrical particle shifting
WO2016019393A1 (en) * 2014-08-01 2016-02-04 Gpb Scientific, Llc Methods and systems for processing particles
US20190366342A1 (en) * 2016-10-24 2019-12-05 Gpb Scientific, Llc Deterministic lateral displacement in the preparation of cells and compositions for therapeutic uses
WO2019222049A1 (en) * 2018-05-13 2019-11-21 Gpb Scientific, Llc Combined purification and concentration by deterministic lateral displacement with recirculation product

Also Published As

Publication number Publication date
CA3166192A1 (en) 2021-07-01
CN115209996A (zh) 2022-10-18
US20230028754A1 (en) 2023-01-26
CN115209996B (zh) 2025-09-05
EP4081344A1 (en) 2022-11-02
KR20220129566A (ko) 2022-09-23
AU2020411478A1 (en) 2022-07-14
WO2021133897A1 (en) 2021-07-01

Similar Documents

Publication Publication Date Title
JP2023508465A (ja) 粒子および細胞を処理するためのマイクロ流体カートリッジ
JP7827107B2 (ja) 生成物の再循環を用いた決定論的横方向変位（Ｄｅｔｅｒｍｉｎｉｓｔｉｃ Ｌａｔｅｒａｌ Ｄｉｓｐｌａｃｅｍｅｎｔ）による結合精製および濃縮
JP2023022241A (ja) 治療用の細胞および組成物の調製における決定論的横置換
AU2019300019B2 (en) Microfluidic methods for the preparation of cells
US20210395682A1 (en) High efficiency microfluidic purification of stem cells to improve transplants
US20220251507A1 (en) Methods for producing cell populations with increased nucleic acid uptake
US20230146950A1 (en) Deterministic lateral displacement array with a single column of bumping obstacles
US20250059506A1 (en) Label-free cell isolation
CN116783283A (zh) 产生具有增加的核酸摄取的细胞群体的方法

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20231221

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20231221

RD03 Notification of appointment of power of attorney

Free format text: JAPANESE INTERMEDIATE CODE: A7423

Effective date: 20241018

A711 Notification of change in applicant

Free format text: JAPANESE INTERMEDIATE CODE: A711

Effective date: 20241021

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A821

Effective date: 20241018

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A821

Effective date: 20241021

A977 Report on retrieval

Free format text: JAPANESE INTERMEDIATE CODE: A971007

Effective date: 20250129

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20250304

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20250425

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20250729

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20250919

A02 Decision of refusal

Free format text: JAPANESE INTERMEDIATE CODE: A02

Effective date: 20251007

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20260106

A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20260331