JP2023503635A5 - - Google Patents

Info

Publication number
JP2023503635A5
JP2023503635A5 JP2022531065A JP2022531065A JP2023503635A5 JP 2023503635 A5 JP2023503635 A5 JP 2023503635A5 JP 2022531065 A JP2022531065 A JP 2022531065A JP 2022531065 A JP2022531065 A JP 2022531065A JP 2023503635 A5 JP2023503635 A5 JP 2023503635A5
Authority
JP
Japan
Prior art keywords
rna
rna fragment
terminal region
sequence
fragment
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP2022531065A
Other languages
English (en)
Japanese (ja)
Other versions
JP7684298B2 (ja
JP2023503635A (ja
Filing date
Publication date
Application filed filed Critical
Priority claimed from PCT/US2020/062342 external-priority patent/WO2021108647A1/en
Publication of JP2023503635A publication Critical patent/JP2023503635A/ja
Publication of JP2023503635A5 publication Critical patent/JP2023503635A5/ja
Application granted granted Critical
Publication of JP7684298B2 publication Critical patent/JP7684298B2/ja
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

JP2022531065A 2019-11-27 2020-11-25 Ｒｎａ分子を合成する方法 Active JP7684298B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201962941174P 2019-11-27 2019-11-27
US62/941,174 2019-11-27
PCT/US2020/062342 WO2021108647A1 (en) 2019-11-27 2020-11-25 Methods of synthesizing rna molecules

Publications (3)

Publication Number Publication Date
JP2023503635A JP2023503635A (ja) 2023-01-31
JP2023503635A5 true JP2023503635A5 (https=) 2023-12-04
JP7684298B2 JP7684298B2 (ja) 2025-05-27

Family

ID=73856352

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2022531065A Active JP7684298B2 (ja) 2019-11-27 2020-11-25 Ｒｎａ分子を合成する方法

Country Status (7)

Country Link
US (1) US20220411841A1 (https=)
EP (1) EP4065711A1 (https=)
JP (1) JP7684298B2 (https=)
CN (1) CN115335521B (https=)
AU (1) AU2020391215A1 (https=)
CA (1) CA3159501A1 (https=)
WO (1) WO2021108647A1 (https=)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20230220379A1 (en) * 2021-09-24 2023-07-13 Crispr Therapeutics Ag HIGH PURITY gRNA SYNTHESIS PROCESS
JPWO2024048684A1 (https=) * 2022-08-31 2024-03-07
CN117660374A (zh) * 2022-09-08 2024-03-08 凯莱英医药集团（天津）股份有限公司 Rna连接酶在寡核苷酸制备上的应用
WO2025114441A1 (en) * 2023-11-30 2025-06-05 F. Hoffmann-La Roche Ag Single guide rna synthesis
WO2025215514A1 (en) * 2024-04-08 2025-10-16 Crispr Therapeutics Ag Splint-mediated rna synthesis

Family Cites Families (83)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US253A (en) 1837-07-05 Improvement in machines for thinning cotton-plants
US196A (en) 1837-05-15 Machine for mowing and heaping grain
US5563A (en) 1848-05-09 John drummond
US562A (en) 1838-01-09 Scale beam and weight
US5177A (en) 1847-06-26 Wade haworth
US5455A (en) 1848-02-22 Spinning-machine
US233A (en) 1837-06-14 Improvement in plows
US5264A (en) 1847-08-28 Apparatus for operating carriage-brakes
US3687808A (en) 1969-08-14 1972-08-29 Univ Leland Stanford Junior Synthetic polynucleotides
US4469863A (en) 1980-11-12 1984-09-04 Ts O Paul O P Nonionic nucleic acid alkyl and aryl phosphonates and processes for manufacture and use thereof
US5023243A (en) 1981-10-23 1991-06-11 Molecular Biosystems, Inc. Oligonucleotide therapeutic agent and method of making same
US4476301A (en) 1982-04-29 1984-10-09 Centre National De La Recherche Scientifique Oligonucleotides, a process for preparing the same and their application as mediators of the action of interferon
US5550111A (en) 1984-07-11 1996-08-27 Temple University-Of The Commonwealth System Of Higher Education Dual action 2',5'-oligoadenylate antiviral derivatives and uses thereof
US5367066A (en) 1984-10-16 1994-11-22 Chiron Corporation Oligonucleotides with selectably cleavable and/or abasic sites
FR2575751B1 (fr) 1985-01-08 1987-04-03 Pasteur Institut Nouveaux nucleosides de derives de l'adenosine, leur preparation et leurs applications biologiques
US5235033A (en) 1985-03-15 1993-08-10 Anti-Gene Development Group Alpha-morpholino ribonucleoside derivatives and polymers thereof
US5185444A (en) 1985-03-15 1993-02-09 Anti-Gene Deveopment Group Uncharged morpolino-based polymers having phosphorous containing chiral intersubunit linkages
US5166315A (en) 1989-12-20 1992-11-24 Anti-Gene Development Group Sequence-specific binding polymers for duplex nucleic acids
US5405938A (en) 1989-12-20 1995-04-11 Anti-Gene Development Group Sequence-specific binding polymers for duplex nucleic acids
US5034506A (en) 1985-03-15 1991-07-23 Anti-Gene Development Group Uncharged morpholino-based polymers having achiral intersubunit linkages
US5264423A (en) 1987-03-25 1993-11-23 The United States Of America As Represented By The Department Of Health And Human Services Inhibitors for replication of retroviruses and for the expression of oncogene products
US5276019A (en) 1987-03-25 1994-01-04 The United States Of America As Represented By The Department Of Health And Human Services Inhibitors for replication of retroviruses and for the expression of oncogene products
EP0366685B1 (en) 1987-06-24 1994-10-19 Howard Florey Institute Of Experimental Physiology And Medicine Nucleoside derivatives
US4924624A (en) 1987-10-22 1990-05-15 Temple University-Of The Commonwealth System Of Higher Education 2,',5'-phosphorothioate oligoadenylates and plant antiviral uses thereof
US5188897A (en) 1987-10-22 1993-02-23 Temple University Of The Commonwealth System Of Higher Education Encapsulated 2',5'-phosphorothioate oligoadenylates
JPH03503894A (ja) 1988-03-25 1991-08-29 ユニバーシィティ　オブ　バージニア　アランミ　パテンツ　ファウンデイション オリゴヌクレオチド　ｎ‐アルキルホスホラミデート
US5278302A (en) 1988-05-26 1994-01-11 University Patents, Inc. Polynucleotide phosphorodithioates
US5216141A (en) 1988-06-06 1993-06-01 Benner Steven A Oligonucleotide analogs containing sulfur linkages
US5175273A (en) 1988-07-01 1992-12-29 Genentech, Inc. Nucleic acid intercalating agents
US5134066A (en) 1989-08-29 1992-07-28 Monsanto Company Improved probes using nucleosides containing 3-dezauracil analogs
US5399676A (en) 1989-10-23 1995-03-21 Gilead Sciences Oligonucleotides with inverted polarity
US5264564A (en) 1989-10-24 1993-11-23 Gilead Sciences Oligonucleotide analogs with novel linkages
US5130302A (en) 1989-12-20 1992-07-14 Boron Bilogicals, Inc. Boronated nucleoside, nucleotide and oligonucleotide compounds, compositions and methods for using same
US5459255A (en) 1990-01-11 1995-10-17 Isis Pharmaceuticals, Inc. N-2 substituted purines
US5681941A (en) 1990-01-11 1997-10-28 Isis Pharmaceuticals, Inc. Substituted purines and oligonucleotide cross-linking
US5587470A (en) 1990-01-11 1996-12-24 Isis Pharmaceuticals, Inc. 3-deazapurines
US5587361A (en) 1991-10-15 1996-12-24 Isis Pharmaceuticals, Inc. Oligonucleotides having phosphorothioate linkages of high chiral purity
US5321131A (en) 1990-03-08 1994-06-14 Hybridon, Inc. Site-specific functionalization of oligodeoxynucleotides for non-radioactive labelling
US5470967A (en) 1990-04-10 1995-11-28 The Dupont Merck Pharmaceutical Company Oligonucleotide analogs with sulfamate linkages
US5602240A (en) 1990-07-27 1997-02-11 Ciba Geigy Ag. Backbone modified oligonucleotide analogs
US5489677A (en) 1990-07-27 1996-02-06 Isis Pharmaceuticals, Inc. Oligonucleoside linkages containing adjacent oxygen and nitrogen atoms
JPH0874B2 (ja) 1990-07-27 1996-01-10 アイシス・ファーマシューティカルス・インコーポレーテッド 遺伝子発現を検出および変調するヌクレアーゼ耐性、ピリミジン修飾オリゴヌクレオチド
US5623070A (en) 1990-07-27 1997-04-22 Isis Pharmaceuticals, Inc. Heteroatomic oligonucleoside linkages
US5541307A (en) 1990-07-27 1996-07-30 Isis Pharmaceuticals, Inc. Backbone modified oligonucleotide analogs and solid phase synthesis thereof
US5608046A (en) 1990-07-27 1997-03-04 Isis Pharmaceuticals, Inc. Conjugated 4'-desmethyl nucleoside analog compounds
US5610289A (en) 1990-07-27 1997-03-11 Isis Pharmaceuticals, Inc. Backbone modified oligonucleotide analogues
US5618704A (en) 1990-07-27 1997-04-08 Isis Pharmacueticals, Inc. Backbone-modified oligonucleotide analogs and preparation thereof through radical coupling
US5677437A (en) 1990-07-27 1997-10-14 Isis Pharmaceuticals, Inc. Heteroatomic oligonucleoside linkages
MY107332A (en) 1990-08-03 1995-11-30 Sterling Drug Inc Compounds and methods for inhibiting gene expression.
US5214134A (en) 1990-09-12 1993-05-25 Sterling Winthrop Inc. Process of linking nucleosides with a siloxane bridge
US5561225A (en) 1990-09-19 1996-10-01 Southern Research Institute Polynucleotide analogs containing sulfonate and sulfonamide internucleoside linkages
JPH06505704A (ja) 1990-09-20 1994-06-30 ギリアド　サイエンシズ，インコーポレイテッド 改変ヌクレオシド間結合
US5432272A (en) 1990-10-09 1995-07-11 Benner; Steven A. Method for incorporating into a DNA or RNA oligonucleotide using nucleotides bearing heterocyclic bases
US5714331A (en) 1991-05-24 1998-02-03 Buchardt, Deceased; Ole Peptide nucleic acids having enhanced binding affinity, sequence specificity and solubility
US5719262A (en) 1993-11-22 1998-02-17 Buchardt, Deceased; Ole Peptide nucleic acids having amino acid side chains
US5539082A (en) 1993-04-26 1996-07-23 Nielsen; Peter E. Peptide nucleic acids
US5571799A (en) 1991-08-12 1996-11-05 Basco, Ltd. (2'-5') oligoadenylate analogues useful as inhibitors of host-v5.-graft response
US5484908A (en) 1991-11-26 1996-01-16 Gilead Sciences, Inc. Oligonucleotides containing 5-propynyl pyrimidines
TW393513B (en) 1991-11-26 2000-06-11 Isis Pharmaceuticals Inc Enhanced triple-helix and double-helix formation with oligomers containing modified pyrimidines
US5633360A (en) 1992-04-14 1997-05-27 Gilead Sciences, Inc. Oligonucleotide analogs capable of passive cell membrane permeation
US5434257A (en) 1992-06-01 1995-07-18 Gilead Sciences, Inc. Binding compentent oligomers containing unsaturated 3',5' and 2',5' linkages
US5476925A (en) 1993-02-01 1995-12-19 Northwestern University Oligodeoxyribonucleotides including 3'-aminonucleoside-phosphoramidate linkages and terminal 3'-amino groups
GB9304618D0 (en) 1993-03-06 1993-04-21 Ciba Geigy Ag Chemical compounds
HU9501974D0 (en) 1993-03-31 1995-09-28 Sterling Winthrop Inc Oligonucleotides with amide linkages replacing phosphodiester linkages
US5502177A (en) 1993-09-17 1996-03-26 Gilead Sciences, Inc. Pyrimidine derivatives for labeled binding partners
US5457187A (en) 1993-12-08 1995-10-10 Board Of Regents University Of Nebraska Oligonucleotides containing 5-fluorouracil
US5596091A (en) 1994-03-18 1997-01-21 The Regents Of The University Of California Antisense oligonucleotides comprising 5-aminoalkyl pyrimidine nucleotides
US5625050A (en) 1994-03-31 1997-04-29 Amgen Inc. Modified oligonucleotides and intermediates useful in nucleic acid therapeutics
US5525711A (en) 1994-05-18 1996-06-11 The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services Pteridine nucleotide analogs as fluorescent DNA probes
JP3756313B2 (ja) 1997-03-07 2006-03-15 武 今西 新規ビシクロヌクレオシド及びオリゴヌクレオチド類縁体
JP4236812B2 (ja) 1997-09-12 2009-03-11 エクシコン エ／エス オリゴヌクレオチド類似体
US20030158403A1 (en) 2001-07-03 2003-08-21 Isis Pharmaceuticals, Inc. Nuclease resistant chimeric oligonucleotides
US8697359B1 (en) 2012-12-12 2014-04-15 The Broad Institute, Inc. CRISPR-Cas systems and methods for altering expression of gene products
US9828600B2 (en) * 2013-09-20 2017-11-28 University Of Massachusetts Compositions and methods for constructing cDNA libraries that allow for mapping the 5′ and 3′ ends of RNAs
ES2781323T3 (es) 2014-06-23 2020-09-01 Regeneron Pharma Montaje de ADN mediado por nucleasas
WO2016061523A1 (en) 2014-10-17 2016-04-21 Howard Hughes Medical Institute Genomic probes
US10059940B2 (en) * 2015-01-27 2018-08-28 Minghong Zhong Chemically ligated RNAs for CRISPR/Cas9-lgRNA complexes as antiviral therapeutic agents
JP2018515142A (ja) * 2015-05-15 2018-06-14 ダーマコン，インコーポレイテッド． Ｃａｓ９介在遺伝子編集用の合成シングルガイドｒｎａ
EP3443088B1 (en) * 2016-04-13 2024-09-18 Editas Medicine, Inc. Grna fusion molecules, gene editing systems, and methods of use thereof
AU2017286835B2 (en) 2016-06-29 2023-12-14 Crispr Therapeutics Ag Compositions and methods for gene editing
WO2018094356A2 (en) * 2016-11-18 2018-05-24 Genedit Inc. Compositions and methods for target nucleic acid modification
SG10202106058WA (en) * 2016-12-08 2021-07-29 Intellia Therapeutics Inc Modified guide rnas
CN111801427B (zh) * 2018-02-05 2023-12-05 豪夫迈·罗氏有限公司 用于单分子的单链环状dna模板的产生

Similar Documents

Publication Publication Date Title
JP2023503635A5 (https=)
CA2220418C (en) Oligonucleotide sizing using cleavable primers
US20260117285A1 (en) Compositions and methods for improving library enrichment
JP2010248084A (ja) 新規洗浄溶媒を用いるオリゴヌクレオチド合成法
CA2476564A1 (en) Polynomial amplification of nucleic acids
CN115335521A (zh) 合成rna分子的方法
US7534872B2 (en) Compositions and methods for the use of FMOC derivatives in DNA/RNA synthesis
KR20260018099A (ko) 뉴클레오티드 사슬을 합성하기 위한 조성물 및 뉴클레오티드 사슬의 합성 방법
JP2024112974A (ja) 改変された酸化プロトコルを使用してオリゴヌクレオチドを調製するための方法
JP2023535287A (ja) ＴｄＴに基づく酵素的核酸の基質としての塩基修飾ヌクレオチド
Sørensen et al. Branched oligonucleotides containing bicyclic nucleotides as branching points and DNA or LNA as triplex forming branch
JP6429264B2 (ja) ボラノホスフェート化合物、及び核酸オリゴマー
JP2023067467A (ja) ３’－エキソヌクレアーゼを用いた長鎖核酸の精製法
JP2024138694A (ja) ヘアピン核酸を製造する方法
US20250171820A1 (en) Method for producing oligonucleic acid
Dolinnaya et al. Chemical ligation as a method for the assembly of double-stranded nucleic acids: Modifications and local structure studies
Su Solid-Phase Synthesis of Boranophosphate/Phosphorothioate/Phosphate Chimeric Oligonucleotides, and Oligonucleotides Containing Phosphorothioate, and 2′-Amino-5′‑S‑phosphorothiolate Linkages
US9890190B2 (en) Preparation of long synthetic oligonucleotides by squarate conjugation chemistry
WO2025114441A1 (en) Single guide rna synthesis
CN119173522A (zh) 方法和化合物
HK40060364A (en) Process for the preparation of oligonucleotides using modified oxidation protocol
Lawrence et al. [3] Sequencing products of polymerase chain reaction
JPH04330093A (ja) オリゴリボヌクレオチドの製造法