JP2023179432A5 - - Google Patents
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- JP2023179432A5 JP2023179432A5 JP2023137627A JP2023137627A JP2023179432A5 JP 2023179432 A5 JP2023179432 A5 JP 2023179432A5 JP 2023137627 A JP2023137627 A JP 2023137627A JP 2023137627 A JP2023137627 A JP 2023137627A JP 2023179432 A5 JP2023179432 A5 JP 2023179432A5
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- eukaryotic cell
- cell
- dna editing
- modified eukaryotic
- rna
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- 210000003527 eukaryotic cell Anatomy 0.000 claims 24
- 238000010442 DNA editing Methods 0.000 claims 14
- 239000003795 chemical substances by application Substances 0.000 claims 9
- 239000004055 small Interfering RNA Substances 0.000 claims 8
- 230000009368 gene silencing by RNA Effects 0.000 claims 7
- 108091032973 (ribonucleotides)n+m Proteins 0.000 claims 5
- 238000012228 RNA interference-mediated gene silencing Methods 0.000 claims 3
- 238000003556 assay Methods 0.000 claims 3
- 108091033409 CRISPR Proteins 0.000 claims 2
- 108020004414 DNA Proteins 0.000 claims 2
- 108010042407 Endonucleases Proteins 0.000 claims 2
- 102000004533 Endonucleases Human genes 0.000 claims 2
- 108090000790 Enzymes Proteins 0.000 claims 2
- 102000004190 Enzymes Human genes 0.000 claims 2
- 108010043121 Green Fluorescent Proteins Proteins 0.000 claims 2
- 102000004144 Green Fluorescent Proteins Human genes 0.000 claims 2
- 108700011259 MicroRNAs Proteins 0.000 claims 2
- 108091028043 Nucleic acid sequence Proteins 0.000 claims 2
- 108091007412 Piwi-interacting RNA Proteins 0.000 claims 2
- 108091027967 Small hairpin RNA Proteins 0.000 claims 2
- 108020004459 Small interfering RNA Proteins 0.000 claims 2
- 238000010459 TALEN Methods 0.000 claims 2
- 108010043645 Transcription Activator-Like Effector Nucleases Proteins 0.000 claims 2
- 108010017070 Zinc Finger Nucleases Proteins 0.000 claims 2
- 210000004027 cell Anatomy 0.000 claims 2
- 238000012217 deletion Methods 0.000 claims 2
- 238000001514 detection method Methods 0.000 claims 2
- 239000005090 green fluorescent protein Substances 0.000 claims 2
- 210000002865 immune cell Anatomy 0.000 claims 2
- 238000000034 method Methods 0.000 claims 2
- 239000002679 microRNA Substances 0.000 claims 2
- 238000003753 real-time PCR Methods 0.000 claims 2
- 241000251468 Actinopterygii Species 0.000 claims 1
- 241000271566 Aves Species 0.000 claims 1
- 238000010354 CRISPR gene editing Methods 0.000 claims 1
- 241000938605 Crocodylia Species 0.000 claims 1
- 241000238424 Crustacea Species 0.000 claims 1
- 241000195493 Cryptophyta Species 0.000 claims 1
- 238000001712 DNA sequencing Methods 0.000 claims 1
- 241000233866 Fungi Species 0.000 claims 1
- 108020005004 Guide RNA Proteins 0.000 claims 1
- 241000238631 Hexapoda Species 0.000 claims 1
- 241000124008 Mammalia Species 0.000 claims 1
- 241000244206 Nematoda Species 0.000 claims 1
- 238000000636 Northern blotting Methods 0.000 claims 1
- 238000003559 RNA-seq method Methods 0.000 claims 1
- 102000006382 Ribonucleases Human genes 0.000 claims 1
- 108010083644 Ribonucleases Proteins 0.000 claims 1
- 238000002105 Southern blotting Methods 0.000 claims 1
- 210000001744 T-lymphocyte Anatomy 0.000 claims 1
- 238000004458 analytical method Methods 0.000 claims 1
- 210000003719 b-lymphocyte Anatomy 0.000 claims 1
- 238000003776 cleavage reaction Methods 0.000 claims 1
- 230000037430 deletion Effects 0.000 claims 1
- 238000001962 electrophoresis Methods 0.000 claims 1
- 230000002538 fungal effect Effects 0.000 claims 1
- 230000030279 gene silencing Effects 0.000 claims 1
- 238000004128 high performance liquid chromatography Methods 0.000 claims 1
- 210000005260 human cell Anatomy 0.000 claims 1
- 238000009396 hybridization Methods 0.000 claims 1
- 238000007901 in situ hybridization Methods 0.000 claims 1
- 238000003780 insertion Methods 0.000 claims 1
- 230000037431 insertion Effects 0.000 claims 1
- 210000002540 macrophage Anatomy 0.000 claims 1
- 238000012986 modification Methods 0.000 claims 1
- 230000004048 modification Effects 0.000 claims 1
- 210000000822 natural killer cell Anatomy 0.000 claims 1
- 108090000623 proteins and genes Proteins 0.000 claims 1
- 238000003757 reverse transcription PCR Methods 0.000 claims 1
- 230000007017 scission Effects 0.000 claims 1
- 238000006467 substitution reaction Methods 0.000 claims 1
- 210000003014 totipotent stem cell Anatomy 0.000 claims 1
Claims (15)
- 改変された、自然環境から単離された真核細胞であって、前記真核細胞は植物細胞ではなく、
ここで、真核細胞は、ＤＮＡ編集事象に関してホモ接合又はヘテロ接合であり、
前記ＤＮＡ編集事象は、下記の工程を含む方法を使用する、前記真核細胞における標的ＲＮＡに対する、ＲＮＡサイレンシング分子をコードするか又はそれにプロセシングされる遺伝子の改変であり、
前記方法は、自然環境から単離された真核細胞にＤＮＡ編集剤を導入する工程であって、前記ＤＮＡ編集剤は、ＲＮＡサイレンシング分子のサイレンシング特異性を第２の標的ＲＮＡに向けてリダイレクトするＤＮＡ編集剤であり、前記標的ＲＮＡと前記第２の標的ＲＮＡは異なり、
それにより、前記ＲＮＡサイレンシング分子をコードする前記遺伝子を、ＲＮＡサイレンシング分子の元の特異性が破壊され、第２の標的ＲＮＡへの新しい特異性が獲得される工程を含み、
前記ＤＮＡ編集剤はエンドヌクレアーゼ及び少なくとも１つのｇＲＮＡを含む、改変された真核細胞。 - 前記ＤＮＡ編集事象が挿入、欠失、挿入－欠失、反転、置換又はそれらの組み合わせである、請求項１に記載の改変された真核細胞。
- ＤＮＡ編集剤をコードするＤＮＡ配列を含まない細胞である、請求項１又は２に記載の改変された真核細胞。
- ＤＮＡ編集剤をコードするＤＮＡ配列の不存在が、ＤＮＡ編集剤の発現の喪失によって検出される、請求項３に記載の改変された真核細胞。
- ＤＮＡ編集剤の発現の喪失が、緑色蛍光タンパク質（ＧＦＰ）の蛍光検出、定量ポリメラーゼ連鎖反応（ｑ－ＰＣＲ）又は高速液体クロマトグラフィー（ＨＰＬＣ）によって決定される、請求項４に記載の改変された真核細胞。
- 前記ＤＮＡ編集事象が、ＤＮＡ及びＲＮＡ配列決定、電気泳動、酵素に基づくミスマッチ検出アッセイ、ハイブリダイゼーションアッセイ、例えば、ＰＣＲ、ＲＴ－ＰＣＲ、ＲＮａｓｅ保護、ｉｎ－ｓｉｔｕハイブリダイゼーション、プライマー伸長、サザンブロット、ノーザンブロット、ドットブロット分析又はミスマッチＤＮＡを認識し切断する構造選択的酵素を利用するミスマッチ切断アッセイにより検出される、請求項１～５のいずれか一項に記載の改変された真核細胞。
- 真核細胞が、哺乳動物、昆虫、線虫、鳥類、爬虫類、魚類、甲殻類、真菌及び藻類からなる群から選択される真菌生物から得られる、請求項１～５のいずれか一項に記載の改変された真核細胞。
- 真核細胞がヒト細胞である、請求項１～５のいずれか一項に記載の改変された真核細胞。
- 真核細胞が全能性幹細胞である、請求項１～５のいずれか一項に記載の改変された真核細胞。
- 真核細胞が免疫細胞である、請求項１～５のいずれか一項に記載の改変された真核細胞。
- 免疫細胞が、Ｔ細胞、Ｂ細胞、マクロファージ又はＮＫ細胞である、請求項１０に記載の改変された真核細胞。
- ＲＮＡサイレンシング分子がＲＮＡ干渉（ＲＮＡｉ）分子である、請求項１～１１のいずれか一項に記載の改変された真核細胞。
- ＲＮＡｉ分子が、低分子干渉ＲＮＡ（ｓｉＲＮＡ）、短鎖ヘアピンＲＮＡ（ｓｈＲＮＡ）、マイクロＲＮＡ（ｍｉＲＮＡ）、Ｐｉｗｉ相互作用ＲＮＡ（ｐｉＲＮＡ）及びトランス作用性ｓｉＲＮＡ（ｔａｓｉＲＮＡ）からなる群から選択される、請求項１２に記載の改変された真核細胞。
- ＤＮＡ編集剤が、メガヌクレアーゼ、ジンクフィンガーヌクレアーゼ（ＺＦＮ）、転写活性化因子様エフェクターヌクレアーゼ（ＴＡＬＥＮ）及びＣＲＩＳＰＲからなる群から選択されるＤＮＡ編集システムを含む、請求項１～１３のいずれか一項に記載の改変された真核細胞。
- エンドヌクレアーゼがＣａｓ９を含む、請求項１～１４のいずれか一項に記載の改変された真核細胞。
Applications Claiming Priority (8)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GBGB1715113.5A GB201715113D0 (en) | 2017-09-19 | 2017-09-19 | Modifying the specificity of plant non-coding RNA molecules for silencing of target RNA |
| GB1715113.5 | 2017-09-19 | ||
| GB1715116.8 | 2017-09-19 | ||
| GBGB1715116.8A GB201715116D0 (en) | 2017-09-19 | 2017-09-19 | Modifying the specificity of non-coding RNA molecules for silencing of target RNA in eukaryotic cells |
| GB1719516.5 | 2017-11-23 | ||
| GBGB1719516.5A GB201719516D0 (en) | 2017-11-23 | 2017-11-23 | Modifying the specificity of plant non-coding RNA molecules for silencing of target RNA |
| PCT/IB2018/057143 WO2019058253A1 (en) | 2017-09-19 | 2018-09-18 | MODIFICATION OF THE SPECIFICITY OF NON-CODING RNA MOLECULES FOR SILENCING GENE EXPRESSION IN EUKARYOTIC CELLS |
| JP2020515764A JP7422069B2 (ja) | 2017-09-19 | 2018-09-18 | 真核細胞において遺伝子発現をサイレンシングするための非コードｒｎａ分子の特異性の改変 |
Related Parent Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2020515764A Division JP7422069B2 (ja) | 2017-09-19 | 2018-09-18 | 真核細胞において遺伝子発現をサイレンシングするための非コードｒｎａ分子の特異性の改変 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2023179432A JP2023179432A (ja) | 2023-12-19 |
| JP2023179432A5 true JP2023179432A5 (ja) | 2024-04-22 |
Family
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Family Applications (3)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2020515770A Active JP7462553B2 (ja) | 2017-09-19 | 2018-09-18 | 遺伝子発現をサイレンシングするための植物非コードｒｎａ分子の特異性の改変 |
| JP2020515764A Active JP7422069B2 (ja) | 2017-09-19 | 2018-09-18 | 真核細胞において遺伝子発現をサイレンシングするための非コードｒｎａ分子の特異性の改変 |
| JP2023137627A Pending JP2023179432A (ja) | 2017-09-19 | 2023-08-25 | 真核細胞において遺伝子発現をサイレンシングするための非コードｒｎａ分子の特異性の改変 |
Family Applications Before (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2020515770A Active JP7462553B2 (ja) | 2017-09-19 | 2018-09-18 | 遺伝子発現をサイレンシングするための植物非コードｒｎａ分子の特異性の改変 |
| JP2020515764A Active JP7422069B2 (ja) | 2017-09-19 | 2018-09-18 | 真核細胞において遺伝子発現をサイレンシングするための非コードｒｎａ分子の特異性の改変 |
Country Status (13)
| Country | Link |
|---|---|
| US (4) | US11555199B2 (ja) |
| EP (3) | EP4170029A3 (ja) |
| JP (3) | JP7462553B2 (ja) |
| KR (3) | KR20230050424A (ja) |
| CN (2) | CN111448318A (ja) |
| AU (3) | AU2018336126A1 (ja) |
| BR (2) | BR112020005416A2 (ja) |
| CA (2) | CA3074948A1 (ja) |
| DK (1) | DK3684930T3 (ja) |
| ES (1) | ES2915576T3 (ja) |
| IL (2) | IL273460B1 (ja) |
| SG (2) | SG11202002479XA (ja) |
| WO (2) | WO2019058253A1 (ja) |
Families Citing this family (32)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| BR112020005416A2 (pt) | 2017-09-19 | 2020-09-29 | Tropic Biosciences UK Limited | modificação da especificidade de moléculas de rna não codificante para silenciar expressão gênica em células eucarióticas |
| EP3794130A4 (en) | 2018-05-16 | 2022-07-27 | Synthego Corporation | METHODS AND SYSTEMS FOR DESIGN AND USE OF GUIDE RNA |
| CN110857438B (zh) * | 2018-08-20 | 2022-05-17 | 中国烟草总公司黑龙江省公司牡丹江烟草科学研究所 | 一种高效产生siRNA的烟草花叶病毒基因片段、弱毒疫苗、制备方法及其应用 |
| EP3931321A1 (en) | 2019-03-01 | 2022-01-05 | Syngenta Crop Protection AG | Suppression of target gene expression through genome editing of native mirnas |
| GB201903520D0 (en) * | 2019-03-14 | 2019-05-01 | Tropic Biosciences Uk Ltd | Modifying the specificity of non-coding rna molecules for silencing genes in eukaryotic cells |
| GB201903521D0 (en) | 2019-03-14 | 2019-05-01 | Tropic Biosciences Uk Ltd | No title |
| GB201903519D0 (en) * | 2019-03-14 | 2019-05-01 | Tropic Biosciences Uk Ltd | Introducing silencing activity to dysfunctional rna molecules and modifying their specificity against a gene of interest |
| CN110551719B (zh) * | 2019-07-30 | 2023-06-02 | 中山大学 | 长链非编码rna基因alex1及其在提高水稻白叶枯病抗性中的应用 |
| CN111508558B (zh) * | 2020-03-23 | 2021-12-14 | 广州赛业百沐生物科技有限公司 | 一种基于CRISPR-Cas9技术设计点突变模型的方法及系统 |
| CN111676220B (zh) * | 2020-05-21 | 2022-03-29 | 扬州大学 | 杨树长链非编码RNA lnc11及其应用 |
| WO2022006310A2 (en) * | 2020-06-30 | 2022-01-06 | Arizona Board Of Regents On Behalf Of The University Of Arizona | Compositions for genome editing and methods of use thereof |
| CN114073760B (zh) * | 2020-08-21 | 2024-02-13 | 北京大学 | Rdr蛋白在肿瘤治疗中的应用 |
| US20240000970A1 (en) * | 2020-12-01 | 2024-01-04 | Emendobio Inc. | Differential knockout of a heterozygous allele of lrrk2 |
| CN112359049B (zh) * | 2020-12-10 | 2022-01-28 | 昆明理工大学 | 一种岷江百合几丁质酶基因LrCHI2及其应用 |
| CN112795568B (zh) * | 2021-01-06 | 2023-07-18 | 河南农业大学 | 一种控制玉米雌穗穗粗杂种优势的非编码基因的应用及其前体序列 |
| GB202103256D0 (en) | 2021-03-09 | 2021-04-21 | Tropic Biosciences Uk Ltd | Method for silencing genes |
| CN114134155B (zh) * | 2021-06-29 | 2023-09-12 | 中国农业科学院油料作物研究所 | 一种mlo基因突变体及其制备方法与应用 |
| CN113444727B (zh) * | 2021-06-30 | 2022-06-21 | 中国热带农业科学院热带生物技术研究所 | 一种LncRNA及其应用 |
| WO2023275255A1 (en) | 2021-07-02 | 2023-01-05 | Tropic Biosciences UK Limited | Delay or prevention of browning in banana fruit |
| KR102573952B1 (ko) * | 2021-08-09 | 2023-09-01 | 경상국립대학교산학협력단 | E2와 이의 상동체 동시 타겟 유전자교정 시스템 및 이의 용도 |
| KR102584891B1 (ko) * | 2021-08-09 | 2023-10-04 | 경상국립대학교산학협력단 | GmIPK1 유전자교정 시스템 및 이의 용도 |
| KR102574819B1 (ko) * | 2021-08-09 | 2023-09-04 | 경상국립대학교산학협력단 | P34와 이의 상동체 동시 타겟 유전자교정 시스템 및 이의 용도 |
| KR102573947B1 (ko) * | 2021-08-09 | 2023-09-01 | 경상국립대학교산학협력단 | 콩 유전자교정 효율 증대를 위한 유전자교정 시스템 및 이의 용도 |
| KR102573948B1 (ko) * | 2021-08-09 | 2023-09-01 | 경상국립대학교산학협력단 | Mips1과 이의 상동체 동시 타겟 유전자교정 시스템 및 이의 용도 |
| GB202112865D0 (en) | 2021-09-09 | 2021-10-27 | Tropic Biosciences Uk Ltd | Resistance to black sigatoka disease in banana |
| GB202112866D0 (en) | 2021-09-09 | 2021-10-27 | Tropic Biosciences Uk Ltd | Resistance to fusarium wilt in a banana |
| CN114807128B (zh) * | 2022-03-24 | 2023-09-01 | 四川农业大学 | 一种lncRNA-BTRL及其应用 |
| CN114794029B (zh) * | 2022-06-09 | 2023-02-28 | 上海大学 | 一种计数秀丽隐杆线虫子宫内发育胚胎数目的方法 |
| CN116064644B (zh) * | 2022-10-10 | 2023-10-10 | 中国农业科学院生物技术研究所 | SbAGO1b蛋白及其编码基因在调控植物抗虫性中的应用 |
| CN116103334A (zh) * | 2022-11-16 | 2023-05-12 | 西北农林科技大学 | 一种猕猴桃抗溃疡病基因功能筛选专用瞬时转化体系 |
| CN115976050B (zh) * | 2022-11-16 | 2024-06-18 | 西北农林科技大学 | 猕猴桃感病基因及其适用的vigs沉默体系构建 |
| GB202305021D0 (en) | 2023-04-04 | 2023-05-17 | Tropic Biosciences Uk Ltd | Methods for generating breaks in a genome |
Family Cites Families (73)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| NL154600B (nl) | 1971-02-10 | 1977-09-15 | Organon Nv | Werkwijze voor het aantonen en bepalen van specifiek bindende eiwitten en hun corresponderende bindbare stoffen. |
| NL154598B (nl) | 1970-11-10 | 1977-09-15 | Organon Nv | Werkwijze voor het aantonen en bepalen van laagmoleculire verbindingen en van eiwitten die deze verbindingen specifiek kunnen binden, alsmede testverpakking. |
| NL154599B (nl) | 1970-12-28 | 1977-09-15 | Organon Nv | Werkwijze voor het aantonen en bepalen van specifiek bindende eiwitten en hun corresponderende bindbare stoffen, alsmede testverpakking. |
| US3901654A (en) | 1971-06-21 | 1975-08-26 | Biological Developments | Receptor assays of biologically active compounds employing biologically specific receptors |
| US3853987A (en) | 1971-09-01 | 1974-12-10 | W Dreyer | Immunological reagent and radioimmuno assay |
| US3867517A (en) | 1971-12-21 | 1975-02-18 | Abbott Lab | Direct radioimmunoassay for antigens and their antibodies |
| NL171930C (nl) | 1972-05-11 | 1983-06-01 | Akzo Nv | Werkwijze voor het aantonen en bepalen van haptenen, alsmede testverpakkingen. |
| US3850578A (en) | 1973-03-12 | 1974-11-26 | H Mcconnell | Process for assaying for biologically active molecules |
| US3935074A (en) | 1973-12-17 | 1976-01-27 | Syva Company | Antibody steric hindrance immunoassay with two antibodies |
| US3996345A (en) | 1974-08-12 | 1976-12-07 | Syva Company | Fluorescence quenching with immunological pairs in immunoassays |
| US4034074A (en) | 1974-09-19 | 1977-07-05 | The Board Of Trustees Of Leland Stanford Junior University | Universal reagent 2-site immunoradiometric assay using labelled anti (IgG) |
| US3984533A (en) | 1975-11-13 | 1976-10-05 | General Electric Company | Electrophoretic method of detecting antigen-antibody reaction |
| US4098876A (en) | 1976-10-26 | 1978-07-04 | Corning Glass Works | Reverse sandwich immunoassay |
| US4879219A (en) | 1980-09-19 | 1989-11-07 | General Hospital Corporation | Immunoassay utilizing monoclonal high affinity IgM antibodies |
| CA1192510A (en) | 1981-05-27 | 1985-08-27 | Lawrence E. Pelcher | Rna plant virus vector or portion thereof, a method of construction thereof, and a method of producing a gene derived product therefrom |
| JPS6054684A (ja) | 1983-09-05 | 1985-03-29 | Teijin Ltd | 新規ｄνａ及びハイブリツドｄνａ |
| US5011771A (en) | 1984-04-12 | 1991-04-30 | The General Hospital Corporation | Multiepitopic immunometric assay |
| US4666828A (en) | 1984-08-15 | 1987-05-19 | The General Hospital Corporation | Test for Huntington's disease |
| US4945050A (en) | 1984-11-13 | 1990-07-31 | Cornell Research Foundation, Inc. | Method for transporting substances into living cells and tissues and apparatus therefor |
| CA1288073C (en) | 1985-03-07 | 1991-08-27 | Paul G. Ahlquist | Rna transformation vector |
| US4683202A (en) | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
| US4801531A (en) | 1985-04-17 | 1989-01-31 | Biotechnology Research Partners, Ltd. | Apo AI/CIII genomic polymorphisms predictive of atherosclerosis |
| GB8608850D0 (en) | 1986-04-11 | 1986-05-14 | Diatech Ltd | Packaging system |
| JPS6314693A (ja) | 1986-07-04 | 1988-01-21 | Sumitomo Chem Co Ltd | 植物ウイルスｒｎａベクタ− |
| ES2039474T3 (es) | 1986-12-05 | 1993-10-01 | Ciba-Geigy Ag | Procedimineto mejorado para la transformacion de protoplastos vegetales. |
| US5015580A (en) | 1987-07-29 | 1991-05-14 | Agracetus | Particle-mediated transformation of soybean plants and lines |
| DE3850683T2 (de) | 1987-02-09 | 1994-10-27 | Lubrizol Genetics Inc | Hybrides RNS-Virus. |
| US5316931A (en) | 1988-02-26 | 1994-05-31 | Biosource Genetics Corp. | Plant viral vectors having heterologous subgenomic promoters for systemic expression of foreign genes |
| US5416011A (en) | 1988-07-22 | 1995-05-16 | Monsanto Company | Method for soybean transformation and regeneration |
| US5693507A (en) | 1988-09-26 | 1997-12-02 | Auburn University | Genetic engineering of plant chloroplasts |
| US5272057A (en) | 1988-10-14 | 1993-12-21 | Georgetown University | Method of detecting a predisposition to cancer by the use of restriction fragment length polymorphism of the gene for human poly (ADP-ribose) polymerase |
| US5302523A (en) | 1989-06-21 | 1994-04-12 | Zeneca Limited | Transformation of plant cells |
| US7705215B1 (en) | 1990-04-17 | 2010-04-27 | Dekalb Genetics Corporation | Methods and compositions for the production of stably transformed, fertile monocot plants and cells thereof |
| US5550318A (en) | 1990-04-17 | 1996-08-27 | Dekalb Genetics Corporation | Methods and compositions for the production of stably transformed, fertile monocot plants and cells thereof |
| US5192659A (en) | 1989-08-25 | 1993-03-09 | Genetype Ag | Intron sequence analysis method for detection of adjacent and remote locus alleles as haplotypes |
| JP3209744B2 (ja) | 1990-01-22 | 2001-09-17 | デカルブ・ジェネティクス・コーポレーション | 結実能力のある遺伝子変換コーン |
| US5484956A (en) | 1990-01-22 | 1996-01-16 | Dekalb Genetics Corporation | Fertile transgenic Zea mays plant comprising heterologous DNA encoding Bacillus thuringiensis endotoxin |
| US6403865B1 (en) | 1990-08-24 | 2002-06-11 | Syngenta Investment Corp. | Method of producing transgenic maize using direct transformation of commercially important genotypes |
| US5384253A (en) | 1990-12-28 | 1995-01-24 | Dekalb Genetics Corporation | Genetic transformation of maize cells by electroporation of cells pretreated with pectin degrading enzymes |
| US5591616A (en) | 1992-07-07 | 1997-01-07 | Japan Tobacco, Inc. | Method for transforming monocotyledons |
| US5281521A (en) | 1992-07-20 | 1994-01-25 | The Trustees Of The University Of Pennsylvania | Modified avidin-biotin technique |
| HUT70467A (en) | 1992-07-27 | 1995-10-30 | Pioneer Hi Bred Int | An improved method of agrobactenium-mediated transformation of cultvred soyhean cells |
| US5998193A (en) * | 1994-06-24 | 1999-12-07 | Gene Shears Pty., Ltd. | Ribozymes with optimized hybridizing arms, stems, and loops, tRNA embedded ribozymes and compositions thereof |
| US5693512A (en) | 1996-03-01 | 1997-12-02 | The Ohio State Research Foundation | Method for transforming plant tissue by sonication |
| US5981840A (en) | 1997-01-24 | 1999-11-09 | Pioneer Hi-Bred International, Inc. | Methods for agrobacterium-mediated transformation |
| JP2002534129A (ja) | 1999-01-14 | 2002-10-15 | モンサント テクノロジー エルエルシー | ダイズ形質転換方法 |
| US9567591B2 (en) | 2003-05-15 | 2017-02-14 | Mello Biotechnology, Inc. | Generation of human embryonic stem-like cells using intronic RNA |
| US20050203047A1 (en) | 2004-03-10 | 2005-09-15 | Ulrich Thomann | Delivery vectors for short interfering RNA, micro-RNA and antisense RNA |
| IL179285A (en) | 2004-05-14 | 2011-04-28 | Rosetta Genomics Ltd | Micrornas and uses thereof |
| EP2484758B1 (en) | 2005-10-18 | 2013-10-02 | Precision Biosciences | Rationally-designed meganucleases with altered sequence specificity and DNA-binding affinity |
| US20080280848A1 (en) | 2005-10-28 | 2008-11-13 | Volker Patzel | Structures of Active Guide Rna Molecules and Method of Selection |
| BRPI0814244B1 (pt) | 2007-06-07 | 2018-10-09 | Agriculture And Agri Food Canada | método para obtenção de uma planta geneticamente engenheirada |
| BRPI0817911B8 (pt) | 2007-10-05 | 2022-06-28 | Dow Agrosciences Llc | Processos para transferência de substâncias moleculares para dentro de células vegetais e processo para expressão de um gene |
| US8212019B2 (en) * | 2008-07-30 | 2012-07-03 | University Of Massachusetts | Nucleic acid silencing sequences |
| EP2292176B1 (en) | 2009-09-07 | 2019-01-09 | Nobel Biocare Services AG | Implantation set |
| US9150854B2 (en) * | 2010-12-02 | 2015-10-06 | Keygene N.V. | Targeted alteration of DNA |
| EP2819703A4 (en) | 2012-02-29 | 2015-11-18 | Benitec Biopharma Ltd | TREATMENT OF PAIN |
| US10000753B2 (en) | 2013-01-08 | 2018-06-19 | Benitec Biopharma Limited | Age-related macular degeneration treatment |
| BR112016002385A2 (pt) | 2013-08-07 | 2017-09-12 | Harvard College | animal não humano, célula, tecido ou embrião, vetor de alvejamento, constructo de vetor de alvejamento, e, métodos para produzir um animal não humano e para modificar um lócus de incrna em uma célula pluripotente |
| MX2016002118A (es) | 2013-08-22 | 2016-06-28 | Du Pont | Modificacion del genoma de plantas por medio del uso de sistemas de ácido ribonucléico (arn) guía/ endonucleasa cas y metodos de uso. |
| KR20160089530A (ko) * | 2013-12-12 | 2016-07-27 | 더 브로드 인스티튜트, 인코퍼레이티드 | Ｈｂｖ 및 바이러스 질병 및 질환을 위한 ｃｒｉｓｐｒｃａｓ 시스템 및 조성물의 전달，용도 및 치료적 적용 |
| PL3105328T3 (pl) | 2014-02-11 | 2020-10-19 | The Regents Of The University Of Colorado, A Body Corporate | Umożliwiana przez CRISPR multipleksowa modyfikacja genomu |
| US20160076093A1 (en) | 2014-08-04 | 2016-03-17 | University Of Washington | Multiplex homology-directed repair |
| EP3227447B1 (en) | 2014-12-03 | 2024-04-24 | Agilent Technologies, Inc. | Guide rna with chemical modifications |
| US20170327834A1 (en) | 2014-12-15 | 2017-11-16 | Syngenta Participations Ag | Pesticidal microrna carriers and use thereof |
| CN108368502B (zh) | 2015-06-03 | 2022-03-18 | 内布拉斯加大学董事委员会 | 使用单链dna的dna编辑 |
| CN106701752B (zh) | 2015-09-01 | 2019-07-12 | 清华大学 | 人工合成的microRNA簇及其构建方法与所用合成microRNA单元 |
| CA3011458A1 (en) | 2016-01-14 | 2017-07-20 | The Brigham And Women's Hospital, Inc. | Genome editing for treating glioblastoma |
| CN105647962A (zh) * | 2016-02-15 | 2016-06-08 | 浙江大学 | 运用CRISPR-Cas9系统敲除水稻MIRNA393b茎环序列的基因编辑方法 |
| CN106367435B (zh) | 2016-09-07 | 2019-11-08 | 电子科技大学 | 一种水稻miRNA定向敲除的方法 |
| EP3601579B1 (en) | 2017-03-31 | 2023-10-04 | Pioneer Hi-Bred International, Inc. | Expression modulating elements and use thereof |
| BR112020005416A2 (pt) | 2017-09-19 | 2020-09-29 | Tropic Biosciences UK Limited | modificação da especificidade de moléculas de rna não codificante para silenciar expressão gênica em células eucarióticas |
| EP3931321A1 (en) | 2019-03-01 | 2022-01-05 | Syngenta Crop Protection AG | Suppression of target gene expression through genome editing of native mirnas |
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