JP2023137085A - Dyscerus perforatus (roelofs) repellent and method for using the repellent - Google Patents

Abstract

To develop a Dyscerus perforatus (Roelofs) repellent.SOLUTION: A Dyscerus perforatus (Roelofs) repellent includes at least one compound selected from the group consisting of linear monoterpenes, monocyclic monoterpenes, (1R)-(+)-α-pinene, and orthovanillin as an active ingredient.SELECTED DRAWING: None

Description

The present invention relates to an olive tree weevil repellent and an apparatus used for screening the repellent.

Olive (English name: Olive, scientific name: Olea europaea L.) has been cultivated mainly in the Mediterranean Sea for several thousand years and has been used for food and medicine. Olive oil, which is obtained by pressing the fruit, and useful components such as polyphenols contained in the fruit and leaves are useful for people's diet, health, and preservation of products, so olives are now grown all over the world. It is being done.

After olive trees were first imported to Japan in the late 19th century, olive cultivation began to be carried out mainly on Shodoshima as a national policy from 1908 onwards. When olive planting began in Japan, it became clear that feeding damage was frequently caused by a beetle native to Japan that does not exist in the Mediterranean. perforatus Roelofs).

Since damage caused by the olive burrow weevil causes severe damage to production, countermeasures against the olive burrow weevil have become the biggest challenge for olive producers (Non-Patent Document 1). The current method of dealing with the olive tree weevil is to control it through cultivation by spraying chemicals on the tree trunk and cleaning the base of the plant.

Fenitrothion (Sumithion (registered trademark)), which has a broad insecticidal spectrum, is registered as a drug that can be applied to olive trees. However, even if fenitrothion (Sumithion (registered trademark)) is used in high doses, it cannot be said that the effect of controlling the olive tree weevil is sufficient. Moreover, the use of fenitrothion (Sumithion®) in high doses poses environmental burdens and health risks, such as causing harmful effects on aquatic organisms and destroying the ecosystem, and causing neurotoxicity to humans. However, it is difficult to say that it is socially desirable. In addition, cleaning the base of the plant takes time and effort on the part of the producer, and the time burden of catching and killing the olive burrow weevil is also large.

2-Hydroxy-4-methoxybenzaldehyde is the only chemical substance that has been reported to have a repellent effect on the olive tree weevil (Non-Patent Document 2), but this report contains a large standard deviation and is statistically It was based on data that had not been thoroughly analyzed. Since then, no other chemical substances have been identified that have a repellent effect on the olive tree weevil.

“Research on the control of the olive tree weevil under the Ministry of Agriculture, Forestry and Fisheries Applied Experimental Research Grant,” Hiroshi Matsuzawa, Yuzo Miyamoto, Hidetoshi Okamoto, Yukio Kawahara, Academic Report of the Faculty of Agriculture, Kagawa University, 1957, Vol. 8, No. 2, pp. 172-88 “Repellent from Traditional Chinese Medicine, Periploca sepium Bunge” Jianxiao Shi, Tomoko Yamashita, Ayumi Todo, Teruhiko Nit oda, Minoru Izumi, Naomichi Baba, and Shuhei Nakajima, Zeitschrift für Naturforschung, 2007; 62 (11-12), pages 821-825

An effective and safe control method for the olive tree weevil, which causes serious damage to olive tree growth and productivity improvements, saves labor for growers and farmers, and does not pose an environmental burden or health risk. development is required.

However, to date, only one report has been made regarding a chemical substance that has repellency against the olive weevil, and no device has yet been developed that can appropriately measure the repellency of the olive weevil. be.

Therefore, an object of the present invention is to identify and provide a new effective and safe repellent for the olive tree weevil.

The present inventors have developed a device that can quantitatively measure the repellent effect on the olive weevil in order to identify a new effective and safe repellent for the olive weevil. This device consists of a container having a plurality of compartmentalized spaces, and is a device that makes it possible to determine the olive weevil repelling effect of the test substance by the movement of the olive weevil between the compartments. Using this device, they identified a chemical substance that has a repellent effect on the olive tree weevil. That is, the present invention is as follows.
(Invention 1)
An olive tree weevil containing as an active ingredient at least one or more compounds selected from the group consisting of linear monoterpenes, monocyclic monoterpenes, (1R)-(+)-α-pinene, and orthovanillin. Repellent.
(Invention 2)
The olive tree weevil repellent according to Invention 1, wherein the linear monoterpene is at least one compound selected from the group consisting of geraniol, β-citronellol, citral, and linalool.
(Invention 3)
The olive tree weevil repellent according to invention 2, wherein the linear monoterpene is geraniol.
(Invention 4)
The olive tree weevil repellent of invention 1, wherein the monocyclic monoterpene is at least one compound selected from the group consisting of (-)-limonene, (+)-limonene, and (-)-menthol. .
(Invention 5)
A method for using an olive tree weevil repellent, which comprises scattering, spraying, applying, or placing the olive tree weevil repellent according to any one of Inventions 1 to 4 on or around an olive tree.

The equipment used to complete the present invention is as follows.
(Invention 6)
A device for screening an olive tree weevil repellent, the device comprising:
The device has transparency to the extent that the olive tree weevil staying inside the device can be visually recognized from the outside of the device,
The device has a main body and an opening/closing opening,
The main body is a hollow container for storing a plurality of olive weevils and a test substance,
The main body consists of N sections (N is an integer of 3 or more) connected on the same plane: section #1, section #2, ..., section #N, and the section #1, section #2 ,..., the sections #N are connected in this order,
A gap exists at the boundary between two adjacent sections of the N sections, and at least one olive tree weevil can move between the two adjacent sections by passing through the gap,
The opening/closing opening is arranged at at least one location on the main body,
The test substance and the olive tree weevil can be taken in and out of the device through the opening/closing port,
the device can be sealed by closing the opening;
Device.

The olive tree weevil repellent, which is a monoterpene, orthovanillin, or a mixture containing these, newly identified by the present inventors is safe for humans and does not place a burden on the environment. By scattering, spraying, coating, or placing the repellent of the present invention on or around olive trees, olive trees can be protected from the olive tree weevil while reducing the labor of breeders and farmers, and olive cultivation and growth can be achieved. In addition, it becomes possible to stabilize production and improve olive productivity.

FIG. 1 is a diagram schematically showing an example of the device of the present invention. When the device of the present invention is installed on a flat surface, Figure 1(a) shows the view from above, Figure 1(b) shows the view from the side, and Figure 1(c) shows the view from the end side. , Figure 1(d) is the view from the opening/closing opening side. Graph showing the repellent effect of 2-hydroxy-4-methoxybenzaldehyde against the olive tree weevil. Graph showing the effects of monoterpenes on the behavior of the olive tree weevil. Figure 3(a) shows the effect of geraniol, Figure 3(b) shows the effect of β-citronellol, Figure 3(c) shows the effect of citral, Figure 3(d) shows the effect of linalool, and Figure 3(e) shows the effect of (- )-limonene effect, Figure 3(f) shows the (+)-limonene effect, Figure 3(g) shows the (-)-menthol effect, and Figure 3(h) shows the (1R)-(+)-α- effect. The effect of pinene, Figure 3(i) shows the effect of (1S)-(-)-α-pinene. Graph showing the effect of vanillin analogs on the behavior of the olive tree weevil. Figure 4(a) shows the effect of orthovanillin, and Figure 4(b) shows the effect of vanillin.

[Device]
The device of the invention is a device for screening olive tree weevil repellents. The device of the present invention is required to have transparency to the extent that it is possible to visually check the appearance of the olive tree weevils that remain inside the device of the present invention, such as the location, number of individuals, and body color, from the outside of the device. In order to ensure such transparency, it is preferable that almost the entire container is formed of a transparent material. As long as the above-mentioned transparent material has low chemical reactivity with the test substance described below and does not affect the accuracy of the test using this device, and it can be molded so that the sections described below exist, it is possible to use this device. There are no restrictions as long as it has the strength to withstand operation by a person. Examples of such transparent materials include glass, polyesters such as polyethylene phthalate and polybutylene phthalate, polycarbonate, polyolefins such as polypropylene and polyethylene, polystyrene, and acrylic resins whose main monomer is polymethyl acrylate. Can be used. In terms of ease of handling, a main body made of transparent resin is preferred.

The device of the present invention has a main body and an opening/closing opening. The body is a hollow container for storing a plurality of olive weevils and a test substance.

Consisting of N sections connected on the same plane: section #1, section #2, ..., section #N, and the section #1, section #2, ..., section #N are connected in this order. are doing. The number of sections: N is an integer of 3 or more, preferably 3 or more and 10 or less, and more preferably an integer of 4 or more and 6 or less. The body may have any overall shape. The section #1 and the section #N form both ends of the main body. In said body, one end and the other end are equivalent. The section number assigned to one end of the main body may be #1 or #N.

For example, the main body may be a rod-shaped molded body in which the N sections are connected on the same plane and on the same straight line. In this case, the external appearance of the main body may be a cube, a rectangular parallelepiped, a tube, or the like. Alternatively, for example, the molded body may have a curved portion in which the N sections are connected on the same plane and on the same curve. In this case, the main body may have a U-shape, an S-shape, a ring (doughnut shape), or the like.

In the device of the present invention, a gap exists at the boundary between two adjacent sections of the N sections. In tests using the device of the present invention, at least one olive anatomy weevil can move between the two compartments by passing through the gap. A hole, a slit, a connecting pipe, etc. can be used as a structure for forming such a void. A common example of such a structure is a hole provided in the partition wall between the two adjacent compartments. However, the two adjacent compartments may be connected without substantially providing a partition, and the structure may be such that the olive tree weevil can move from any point in one compartment to the other compartment. In this case, the structure forming the void is an open surface. Further, the structure forming the voids may not be uniform. For example, among two adjacent compartments, one set may be provided with a perforated partition wall, and the other set may be provided with an open surface without a partition wall.

In the device of the present invention, the opening/closing opening is arranged at at least one location on the main body. The test substance and the olive weevil can be taken in and out of the device through the opening, and the device can be sealed by closing the opening.

In a test using the device of the invention, an olive weevil is allowed to encounter the substance to be tested inside a closed body. If the test substance is a substance that the olive weevil does not like, the olive fall weevil avoids the test substance and moves further away from the test substance within the main body. If the test substance is a substance that the olive weevil does not like, the body color of the olive weevil may change (blackening).

In the device of the present invention, a conductor wire for the olive burrow weevil is formed from section #1 to section N of the main body. In a test using the apparatus of the present invention, first, a plurality of olive tree weevils and a test substance are brought close to each other in compartment #1 of the main body. The number of olive weevils to be introduced is appropriately determined in consideration of the capacity of the main body of the present invention and the ease of later counting of the number of individuals.

Usually, a carrier such as filter paper or nonwoven fabric carrying a test substance at a predetermined concentration is brought into close contact with the inner wall of compartment #1. Preferably, a compound with appropriate volatility is selected as the substance to be tested. The olive tree weevil detects the test substance volatilized inside the body.

Thereafter, the olive tree weevil exhibits behaviors such as staying in compartment #1, moving to compartments #2, . . . Eventually, the behavior of the olive tree weevil changes to a state in which it stops moving because it cannot move further away from the test substance, and a state in which it stops moving because the distance to the test substance becomes satisfactory/tolerable. It's extremely stable. In a test using the apparatus of the present invention, the olive weevil and the test substance are brought into close contact with each other in section #1 for several hours, preferably from 1 hour to 5 hours, more preferably from 2 hours to 4 hours, until the main body keep it sealed.

After a predetermined period of time has elapsed, olive burrow weevils are found from outside the device in each of sections #, #2, ... #N of the main body, and the number of olive burrow weevils staying in each section is counted.

The olive weevils that remained in section #1 were staying close to the test substance. The high proportion of the olive weevil individuals in the main body remaining in compartment #1 in the container indicates that the olive weevil's ability to repel the test substance was relatively low.

The olive weevil that has moved to compartment #N has moved to the farthest position from the test substance within the apparatus of the present invention. The high proportion of olive weevil individuals within the main body remaining in compartment #N in the container indicates that the olive weevil had a relatively high ability to repel the test substance. The high proportion of blackened olive weevil individuals in the body indicates that the olive weevil had a relatively high ability to repel the test substance.

The device of the present invention can be manufactured at low cost using commercially available PET bottles. FIG. 1 shows an example of the apparatus of the present invention using a plastic bottle. The dimensions and shapes of various parts shown in FIG. 1 may be exaggerated or omitted. FIG. 1(a) shows the device (100) of the present invention viewed from above when installed on a floor or the ground in a usage environment. FIG. 1(b) shows a side view of the device (100) shown in FIG. 1(a). FIG. 1(c) shows the device (100) viewed from the bottom (41, 42) side. FIG. 1(d) shows the device (100) viewed from the lid (3, 4) side. In FIG. 1(b), objects inside the device (100) are omitted.

The device (100) of the present invention shown in FIG. 1 has a main body (10) and an opening/closing opening (20). The main body (10) is a combination of a plastic bottle (11) and a plastic bottle (12). Both the PET bottle (11) and the PET bottle (12) are 1 liter type commercially available beverage containers. The actual capacity of each is approximately 1323 ^cm3 .

The main body (10) is provided with openings (20) at two locations. One opening/closing opening (3) is provided at the lid of the plastic bottle (11). The other opening/closing opening (4) is provided on the lid of the plastic bottle (12).

The olive weevil (5) inside the plastic bottle (11) and the cartridge 0.5 mL plastic tube (1) containing the filter paper impregnated with the test substance are visible through the transparent body (10). However, in Figure 1, only one individual olive tree weevil is shown, and other individuals are omitted. Figure 1 shows how the olive tree weevil (5) was brought close to the test substance at the beginning of the experiment.

The plastic bottle (11) has a constriction (31), and in the longitudinal direction from the bottom (41) to the opening/closing opening (3), there is a section #1 from the bottom (41) to the constriction (31), and a constriction (31). ) to opening/closing opening (3) #2 is formed. There is no partition wall between compartment #1 and compartment #2, and compartment #1 and compartment #2 are connected via an open surface narrower than the bottom (41) at the constriction (31).

Similarly, the plastic bottle (12) is formed with a compartment #4 from the bottom (42) to the constriction (32), and a compartment #3 from the constriction (32) to the opening/closing opening (4). There is no partition wall between compartment #3 and compartment #4, and compartment #3 and compartment #4 are connected via an open surface narrower than the bottom (42) at the constriction (32).

Section #2 and section #3 are connected via an opening (2). The opening (2) consists of a hole penetrating the wall of the PET bottle (11) and the wall of the PET bottle (12).

Inside the main body (10), there is formed a conductor wire for the olive tree weevil (5) that passes through four compartments: #1, #2, #3, #4 in this order. The olive tree weevil (5), which avoids the test substance, moves from compartment #1 to the neighboring compartment #2, or further away from compartment #1 to compartment #3, or from compartment #1 to the farthest compartment #4. Can be moved. In FIG. 1(a), one example of the movement route of the olive tree weevil (5) is indicated by a dotted line. In reality, such a route cannot be seen.

A user of the apparatus (100) of the present invention measures the number of olive anatomy weevils staying in each of the four compartments: #1, #2, #3, and #4 after a predetermined time after starting the experiment.

In the present invention, measurement results are evaluated by a general statistical method used for determining significance. In the present invention, the measurement results are preferably evaluated by one-way ANOVA. One-way analysis of variance is a statistical method for testing differences in means of three or more groups, usually with a single independent variable or factor, where different levels of variation or the factor have measurable effects on the dependent variable. It is used to check whether it has. Thereafter, a test is performed using Tukey's intergroup comparison.

[Olive weevil repellent]
Using the above device, the inventors investigated the repellent activity of 2-hydroxy-4-methoxybenzaldehyde, which is described as having repellent activity in the prior art document (Non-Patent Document 2). As a result, it was revealed that the repellent effect of 2-hydroxy-4-methoxybenzaldehyde, which could not be confirmed with the device of the prior art document (Non-Patent Document 2), was statistically significant and concentration-dependent (Example 1) It was confirmed that the repellent effect measuring device produced by the present inventors can appropriately evaluate the repellent effect against the olive tree weevil.

Using the above-mentioned apparatus, we measured the repellency of various chemical substances to the olive burrow weevil, and found that monoterpenes exhibited the repellency of the olive burrow weevil. Among them, linear and monocyclic monoterpenes were found to have a strong repellent effect on the olive tree weevil. Among bicyclic monoterpenes, (1R)-(+)-α-pinene was found to have a repellent effect on the olive tree weevil. Furthermore, it was revealed that orthovanillin, for which no repellent effect was confirmed in the prior art document (Non-Patent Document 2), has a statistically significant repellent effect on the olive tree weevil.

By using the above-mentioned device, the olive burrow weevil repellent effect of the olive burrow weevil repellent of the present invention was discovered by verifying its repellent effect with a high degree of precision not found in conventional methods. The active ingredients of the olive tree weevil repellent of the present invention are typically geraniol, β-citronellol, citral, linalool, (-)-limonene, (+)-limonene, (-)-menthol, (1R) It contains at least one of -(+)-α-pinene or orthovanillin as an active ingredient. Geraniol, β-citronellol, citral, and linalool are linear, (-)-limonene, (+)-limonene, and (-)-menthol are monocyclic, and (1R)-(+)-α-pinene is dicyclic. It is a cyclic monoterpene.

All of these chemical substances are natural products that are biosynthesized by plants, and are contained in foods, cosmetics, medicines, essential oils, aroma products, etc., and are deeply ubiquitous in human life and are highly safe. It is.

According to the present invention, the compound that has been newly confirmed to have a repellent effect on the olive tree weevil can be dissolved in a solvent, mixed with a solid carrier, or adsorbed to form a spray, a spray, a coating, a liquid, or a powder. It can be prepared in the form of granules, gels, creams, etc., and used as an olive tree weevil repellent. Further, the olive tree weevil repellent of the present invention also includes a compound that supports a compound that has been confirmed to have a repellent effect and is in a form that can be placed in and around olive trees.

Examples of the solvent that can be used include water, alcohols such as methanol and ethanol, glycols, acetone, ketones such as methyl ethyl ketone, and esters such as ethyl acetate. The use of ethanol is more preferable due to the solubility of chemicals and the impact on humans and the environment. These solvents may be used alone or in combination of two or more.

The solid carrier is generally, for example, mineral or inorganic powder such as talc, clay, diatomaceous earth, calcium carbonate, or silica, vegetable powder such as wood flour, soybean flour, wheat flour, or starch powder, pulp, or phenol. Plastics such as resins, fibers, or rubber powders can be used. These solid carriers may be used alone or in combination of two or more.

The olive tree weevil repellent of the present invention may contain additives such as a film forming agent, a surfactant, a wetting agent, a stabilizer, and a propellant, if necessary. Examples of the coating film forming agent include ethyl cellulose, hydroxypropyl cellulose, silicone, acrylic resin, chlorinated rubber, petroleum resin, methyl cellulose, vinyl acetate, polyvinyl alcohol, and latex emulsion. Examples of the surfactant include aliphatic monocarboxylate, linear alkylbennesulfonate, alkyl sulfate, sorbitan fatty acid ester, polyoxyethylene alkyl ether, polyethylene glycol fatty acid ester, fatty acid alkanolamide, etc. . Examples of wetting agents include casein, gelatin, alginic acid, carboxymethyl cellulose, and the like. Examples of the stabilizer include butylated hydroxyanisole, dibutylated hydroxytoluene, tocopherol, and γ-oryzanol. Examples of the propellant include liquefied petroleum gas, dimethyl ether, fluorocarbon, and compressed gas. These additives may be used alone or in combination of two or more types, but it is preferable to select and use them in consideration of the influence on humans and the environment.

The olive tree weevil repellent of the present invention carries a compound that has been confirmed to have a repellent effect and is in a form that can be placed in and around an olive tree, for example, by wrapping it around the trunk of an olive tree or carrying it on a branch. Examples include, but are not limited to, sheets and paper that can be placed at the base of the plant.

[How to use olive tree weevil repellent]
The olive tree weevil repellent of the invention is applied to olive trees in a manner appropriate to its form. There are no restrictions on the varieties of olives to which the law applies. For example, the olive tree weevil repellent of the present invention may be applied, sprayed, or applied directly to olive seedlings or trees; May be placed.

EXAMPLES Hereinafter, the present invention will be explained in more detail with reference to examples, but the present invention is not limited by the following examples.

[Example 1: Preparation of a device for measuring the repellent effect of olive weevil and confirmation of the repellent effect of 2-hydroxy-4-methoxybenzaldehyde on olive weevil]
An apparatus (100) shown in FIG. 1 was manufactured. The compartment on the side closest to the lid of each of two transparent PET bottle containers with lids (length, width, height dimensions: 7cm x 7cm x 7cm) with a content capacity of approximately 1L divided into two by a central constriction. An opening (vertical/horizontal dimensions: 1.5 cm x 1.5 cm) through which the olive weevil can pass is provided on the side, and the openings are connected facing each other so that one end (of a plastic bottle) They were designated as compartment #1, compartment #2, compartment #3, and compartment #4 in order from the bottom (FIG. 1(a)). Five holes with a diameter of 3 mm were drilled as ventilation holes in the opening/closing part of compartment #3 (4 in FIG. 1(a)). A 0.5 mL plastic tube into which a filter paper (vertical and horizontal dimensions: 1 cm x 1 cm) soaked with each dose of the test substance was inserted was placed in compartment #1, and 10 olive tree weevils (body length 12-20 mm) were placed in compartment #4. The cells were placed in a dimly lit room at 20-25°C so that all the compartments were in contact with the floor. After 2 hours of acclimatization, all 10 olive weevils were relocated to plot #1, observed for the next 3 hours, and the number of olive weevils in each plot 3 hours after relocation was recorded. did.

In order to evaluate the repellent effect of the test substance, a point was established for each compartment where the olive tree weevil remained after 3 hours of relocation. Specifically, if the olive tree weevil remained in plot #1, it was given 0 points, if it moved to plot #2, it was 2 points, and if it moved to plot #3 or #4, it was given 3 points. . According to the following formula 1, calculate the repellent activity index (RAI) by multiplying the number of individuals in each compartment by the points assigned to each compartment by the number of individuals in each compartment 3 hours after relocation of the olive tree weevil, and calculate the repellent activity index (RAI). The repellent effect of the test substance was evaluated based on the calculated RAI.

(Formula 1) RAI: (Number of individuals that stayed in #1) x 0 + (Number of individuals that moved to #2) x 2 + (Number of individuals that moved to #3) x 3 + (Number of individuals that moved to #4) x 3
2-Hydroxy-4-methoxybenzaldehyde manufactured by Fuji Film-Wako Pure Chemical Industries, Ltd. was used as the test substance. Filter papers impregnated with 10 mg, 1 mg, or 0.1 mg of 2-hydroxy-4-methoxybenzaldehyde were placed in cartridges and placed in compartment #1. For controls, filter papers impregnated with 20 μL of the ethanol used for dissolution and dilution were placed in 0.5 mL plastic tubes and placed in compartment #1 of the device. One dose per chemical substance per device, and as a control, a filter paper impregnated with 20 μL of ethanol was placed in a 0.5 mL plastic tube in the same manner (2-hydroxy-4-methoxybenzaldehyde 0 mg), and the test was conducted. carried out. A similar test was conducted three times per dose using 10 olive weevils per test. The number of individuals in each plot 3 hours after relocating the olive burrow weevil to plot #1 was recorded, and the distribution of the olive burrow weevil was shown in percentage (%) (Table 1).

The RAI calculated based on the number of individuals in each section of the olive tree weevil was expressed as the mean value ± standard error, and is shown in FIG. 2. After one-way ANOVA (F=7.888, p=9.0×10 ⁻³ ), tests were performed by Tukey's intergroup comparison. Statistically significant differences are indicated in the figure by **; p<0.01.

As a result, it was confirmed that 2-hydroxy-4-methoxybenzaldehyde exhibits a repellent effect in a concentration-dependent manner. It was also revealed that the present repellent effect measuring device functions appropriately and that the olive tree weevil repellent effect of the test substance can be quantified.

[Example 2: Determination of the effect of geraniol as an olive weevil repellent]
The repellent behavior of the olive tree weevil was observed under the same conditions as in Example 1, except that 2-hydroxy-4-methoxybenzaldehyde was replaced with the monoterpene geraniol. Geraniol was purchased from Tokyo Chemical Industry Co., Ltd. The number of individuals in each plot 3 hours after relocating the olive burrow weevil to plot #1 was recorded, and the distribution of the olive burrow weevil was shown in percentage (%) (Table 2).

The RAI calculated based on the number of individuals in each section of the olive tree weevil was expressed as the mean value ± standard error, and is shown in FIG. 3(a). After one-way ANOVA (F=17.12, p=7.7×10 −4 ), tests were performed by Tukey's intergroup comparison. Statistically significant differences are shown in the figures as **; p<0.01, ***; p<0.001.

From this result, the repellent effect of geraniol against the olive tree weevil was confirmed.

[Example 3: Determination of the effect of β-citronellol as an olive weevil repellent]
The repellent behavior of the olive tree weevil was observed under the same conditions as in Example 1, except that 2-hydroxy-4-methoxybenzaldehyde was changed to β-citronellol, a monoterpene. β-citronellol was purchased from Tokyo Chemical Industry Co., Ltd. The number of individuals in each plot 3 hours after relocating the olive burrow weevil to plot #1 was recorded, and the distribution of the olive burrow weevil was shown in percentage (%) (Table 3).

The RAI calculated based on the number of individuals in each section of the olive tree weevil was expressed as the mean value ± standard error, and is shown in FIG. 3(b). After one-way ANOVA (F=11.66, p=2.7×10 ⁻³ ), tests were performed by Tukey's intergroup comparison. Statistically significant differences are indicated in the figure by **; p<0.01.

From this result, the repellent effect of β-citronellol against the olive tree weevil was confirmed.

[Example 4: Determination of the olive tree weevil repellent effect of citral]
The repellent behavior of the olive tree weevil was observed under the same conditions as in Example 1 except that 2-hydroxy-4-methoxybenzaldehyde was changed to citral, a monoterpene. Citral was purchased from Tokyo Chemical Industry Co., Ltd. The number of individuals in each plot 3 hours after relocating the olive burrow weevil to plot #1 was recorded, and the distribution of the olive burrow weevil was shown in percentage (%) (Table 4).

The RAI calculated based on the number of individuals in each section of the olive tree weevil was expressed as the mean value ± standard error, and is shown in FIG. 3(c). After one-way ANOVA (F=17.19, p=7.6×10 ⁻⁴ ), tests were performed by round-robin comparison using Tukey. Statistically significant differences are shown in the figures as *; p<0.05, **; p<0.01.

From this result, the repellent effect of citral against the olive tree weevil was confirmed.

[Example 5: Determination of the olive tree weevil repellent effect of linalool]
The repellent behavior of the olive tree weevil was observed under the same conditions as in Example 1, except that 2-hydroxy-4-methoxybenzaldehyde was changed to the monoterpene linalool. Linalool was purchased from Tokyo Chemical Industry Co., Ltd. The number of individuals in each plot 3 hours after relocating the olive weevil to plot #1 was recorded, and the distribution of the olive weevil was expressed as a percentage (%) (Table 5).

The RAI calculated based on the number of individuals in each section of the olive tree weevil was expressed as the mean value ± standard error, and is shown in FIG. 3(d). After one-way ANOVA (F=16.37, p=8.9×10 ⁻⁴ ), tests were performed by Tukey's intergroup comparison. Statistically significant differences are shown in the figures as *; p<0.05, **; p<0.01.

From this result, the repellent effect of linalool against the olive tree weevil was confirmed.

[Example 6: (-)-Determination of the olive tree weevil repellent effect of limonene]
The repellent behavior of the olive tree weevil was observed under the same conditions as in Example 1, except that 2-hydroxy-4-methoxybenzaldehyde was changed to (-)-limonene, a monoterpene. (-)-Limonene was purchased from Tokyo Chemical Industry Co., Ltd. The number of individuals in each plot 3 hours after relocating the olive burrow weevil to plot #1 was recorded, and the distribution of the olive burrow weevil was expressed as a percentage (%) (Table 6).

The RAI calculated based on the number of individuals in each section of the olive tree weevil was expressed as the mean value ± standard error, and is shown in FIG. 3. After one-way ANOVA (F=19.84, p=4.6×10 ⁻⁴ ), tests were performed by Tukey's intergroup comparison. Statistically significant differences are shown in the figures as *; p<0.05, **; p<0.01, ***; p<0.001.

From this result, the repellent effect of (-)-limonene against the olive tree weevil was confirmed.

[Example 7: Determination of the olive tree weevil repellent effect of (+)-limonene]
The repellent behavior of the olive tree weevil was observed under the same conditions as in Example 1, except that 2-hydroxy-4-methoxybenzaldehyde was changed to (+)-limonene, a monoterpene. (+)-limonene was purchased from Tokyo Chemical Industry Co., Ltd. The number of individuals in each plot 3 hours after relocating the olive burrow weevil to plot #1 was recorded, and the distribution of the olive burrow weevil was shown in percentage (%) (Table 7).

The RAI calculated based on the number of individuals in each section of the olive tree weevil was expressed as the mean value ± standard error, and is shown in FIG. 3(f). After one-way ANOVA (F=26.93, p=1.6x10 ^-4 ), tests were performed by Tukey's intergroup comparison. Statistically significant differences are shown in the figures as *; p<0.05, **; p<0.01, ***; p<0.001.

From this result, the repellent effect of (+)-limonene against the olive tree weevil was confirmed.

[Example 8: (-)-Judgment of menthol's repellent effect on olive tree weevil]
The repellent behavior of the olive tree weevil was observed under the same conditions as in Example 1, except that 2-hydroxy-4-methoxybenzaldehyde was changed to (-)-menthol, a monoterpene. (-)-Menthol was purchased from Tokyo Chemical Industry Co., Ltd. The number of individuals in each plot 3 hours after relocating the olive burrow weevil to plot #1 was recorded, and the distribution of the olive burrow weevil was expressed as a percentage (%) (Table 8).

The RAI calculated based on the number of individuals in each section of the olive tree weevil was expressed as the mean value ± standard error, and is shown in FIG. 3(g). After one-way ANOVA (F=31.59, p=8.8×10 ⁻⁵ ), tests were performed by Tukey's intergroup comparison. Statistically significant differences are indicated in the figure as ***; p<0.001.

From this result, the repellent effect of (-)-menthol against the olive tree weevil was confirmed.

[Example 9: Determination of the olive tree weevil repellent effect of (1R)-(+)-α-pinene]
The repellent behavior of the olive tree weevil was observed under the same conditions as in Example 1, except that 2-hydroxy-4-methoxybenzaldehyde was changed to the monoterpene (1R)-(+)-α-pinene. (1R)-(+)-α-pinene was purchased from Tokyo Chemical Industry Co., Ltd. The number of individuals in each plot 3 hours after relocating the olive burrow weevil to plot #1 was recorded, and the distribution of the olive burrow weevil was shown as a percentage (%) (Table 9).

The RAI calculated based on the number of individuals in each section of the olive tree weevil was expressed as the mean value ± standard error, and is shown in FIG. 3(h). After one-way ANOVA (F=5.768, p=0.021), tests were performed by Tukey's intergroup comparison. Statistically significant differences are indicated in the figure by *; p<0.05.

From this result, the repellent effect of (1R)-(+)-α-pinene against the olive tree weevil was confirmed.
[Example 10: Determination of the olive tree weevil repellent effect of orthovanilin]
The repellent behavior of the olive tree weevil was observed under the same conditions as in Example 1 except that 2-hydroxy-4-methoxybenzaldehyde was changed to orthovanillin. Orthovanillin was purchased from Tokyo Kasei Kogyo Co., Ltd. The number of individuals in each plot 3 hours after relocating the olive burrow weevil to plot #1 was recorded, and the distribution of the olive burrow weevil was shown in percentage (%) (Table 10).

The RAI calculated based on the number of individuals in each section of the olive tree weevil was expressed as the mean value ± standard error, and is shown in FIG. 4(a). After one-way ANOVA (F=112.9, p=6.9×10 ⁻⁷ ), tests were performed by Tukey's intergroup comparison. Statistically significant differences are indicated in the figure as ***; p<0.001.

From this result, the repellent effect of orthovanillin against the olive tree weevil was confirmed.

[Example 11: Determination of the olive tree weevil repellent effect of (1S)-(-)-α-pinene]
The repellent behavior of the olive tree weevil was observed under the same conditions as in Example 1, except that 2-hydroxy-4-methoxybenzaldehyde was changed to the monoterpene (1S)-(-)-α-pinene. (1S)-(-)-α-pinene was purchased from Tokyo Chemical Industry Co., Ltd. The number of individuals in each plot 3 hours after relocating the olive burrow weevil to plot #1 was recorded, and the distribution of the olive burrow weevil was shown in percentage (%) (Table 11).

The RAI calculated based on the number of individuals in each section of the olive tree weevil was expressed as the mean value ± standard error, and is shown in FIG. 3(i). Although one-way ANOVA was performed (F=1.650, p=0.254), no statistically significant difference could be confirmed.

[Example 12: Determination of the effect of vanillin on repelling the olive tree weevil]
The repellent behavior of the olive tree weevil was observed under the same conditions as in Example 1 except that 2-hydroxy-4-methoxybenzaldehyde was replaced with vanillin. Vanillin was purchased from Tokyo Chemical Industry Co., Ltd. The number of individuals in each plot 3 hours after relocating the olive burrow weevil to plot #1 was recorded, and the distribution of the olive burrow weevil was shown in percentage (%) (Table 12).

The RAI calculated based on the number of individuals in each section of the olive tree weevil was expressed as the mean value ± standard error, and is shown in FIG. 4(b). Although one-way ANOVA (F=4.093, p=0.049) was below the significance level (p=0.05), Tukey's between-group comparison showed that 10 mg The p value was 0.053 for each group, and no significant difference was observed between the test substance dose groups.

The olive tree weevil repellent of the present invention contains active ingredients that are safe for humans and do not burden the environment. By scattering, spraying, coating, or placing the repellent of the present invention on or around olive trees, olive trees can be protected from the olive tree weevil while reducing the labor of breeders and farmers, and olive cultivation and growth can be achieved. In addition, it becomes possible to stabilize olive production and improve olive productivity. Moreover, since the repellent of the present invention can be safely and easily applied to olive trees, it contributes to the growth of olive trees not only in farms but also in home gardens, hedges, etc. Furthermore, by using the olive tree weevil repellent activity measuring device developed by the present inventors, it becomes possible to screen for chemical substances having new repellent activity.

1 0.5 mL plastic tube containing filter paper impregnated with test substance 2 Opening 3 Opening/closing opening 4 Opening/closing opening 5 Olive tree weevil 10 Main body 11 PET bottle 12 PET bottle 20 Opening/closing opening 31 Neck of PET bottle 32 Opening/closing of PET bottle Constriction 41 Bottom of PET bottle 42 Bottom of PET bottle 100 Device

Claims (5)

An olive tree weevil containing as an active ingredient at least one or more compounds selected from the group consisting of linear monoterpenes, monocyclic monoterpenes, (1R)-(+)-α-pinene, and orthovanillin. Repellent. The olive tree weevil repellent according to claim 1, wherein the linear monoterpene is at least one compound selected from the group consisting of geraniol, β-citronellol, citral, and linalool. The olive tree weevil repellent according to claim 2, wherein the linear monoterpene is geraniol. The olive anaaki according to claim 1, wherein the monocyclic monoterpene is at least one compound selected from the group consisting of (-)-limonene, (+)-limonene, and (-)-menthol. Weevil repellent. A method for using an olive weevil repellent, which comprises scattering, spraying, applying, or placing the olive weevil repellent according to any one of claims 1 to 4 on or around an olive tree.

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