JP2022537278A - 帯電表面を使用して誘導多能性幹細胞から複数の系統を産生するための方法 - Google Patents

帯電表面を使用して誘導多能性幹細胞から複数の系統を産生するための方法 Download PDF

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JP2022537278A
JP2022537278A JP2021573777A JP2021573777A JP2022537278A JP 2022537278 A JP2022537278 A JP 2022537278A JP 2021573777 A JP2021573777 A JP 2021573777A JP 2021573777 A JP2021573777 A JP 2021573777A JP 2022537278 A JP2022537278 A JP 2022537278A
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ipscs
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ディーピカ ラジェシュ
クリスティー マン
サラ バートン
マデリン ゴードランド
マイケル マクラカン
アビー ムシンスキー
キ ヘ キム
マイケル ハンコック
マキコ オオシマ
アン ストラウス
サラ ディッカーソン
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フジフィルム セルラー ダイナミクス，インコーポレイテッド
フジフィルム ホールディングス アメリカ コーポレイション
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JP2021573777A 2019-06-14 2020-06-15 帯電表面を使用して誘導多能性幹細胞から複数の系統を産生するための方法 Pending JP2022537278A (ja)

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Application Number Priority Date Filing Date Title
US201962861640P 2019-06-14 2019-06-14
US62/861,640 2019-06-14
US201962865806P 2019-06-24 2019-06-24
US62/865,806 2019-06-24
US202063038564P 2020-06-12 2020-06-12
US63/038,564 2020-06-12
PCT/US2020/037790 WO2020252477A1 (en) 2019-06-14 2020-06-15 Methods for the production of multiple lineages from induced pluripotent stem cells using charged surfaces

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JP2022537278A true JP2022537278A (ja) 2022-08-25
JP2022537278A5 JP2022537278A5 (https=) 2023-06-15

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EP (1) EP3983528A1 (https=)
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KR (1) KR20220032561A (https=)
CN (1) CN114174493B (https=)
AU (1) AU2020293287A1 (https=)
CA (1) CA3143332A1 (https=)
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Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115175986B (zh) * 2020-02-10 2024-11-08 公立大学法人名古屋市立大学 用于诱导多能干细胞向脑毛细血管内皮样细胞分化的涂布剂及其利用
CA3215311A1 (en) 2021-04-01 2022-10-06 The Regents Of The University Of California Genetic modification of mammalian cells to confer resistance to csf1r antagonists
US20240191186A1 (en) * 2021-05-05 2024-06-13 FUJIFILM Cellular Dynamics, Inc. Methods and compositions for ipsc-derived microglia
JP2023140135A (ja) 2022-03-22 2023-10-04 株式会社リコー ミクログリアの製造方法
WO2023220453A2 (en) * 2022-05-13 2023-11-16 Herophilus, Inc. Heterogenous neuroimmune organoid model and uses thereof
JP2025522360A (ja) 2022-06-08 2025-07-15 センチュリー セラピューティクス，インコーポレイテッド Ｃｄ１６変異体およびｎｋｇ２ｄを発現する遺伝子操作した細胞ならびにその使用
CN119452078A (zh) * 2022-06-29 2025-02-14 富士胶片控股美国公司 Ipsc来源的星形胶质细胞及其使用方法
TWI820875B (zh) * 2022-08-23 2023-11-01 國璽幹細胞應用技術股份有限公司 優化細胞移植物、其製備方法、及其用途
WO2024102838A1 (en) 2022-11-09 2024-05-16 Century Therapeutics, Inc. Engineered interleukin-7 receptors and uses thereof
JP2026507125A (ja) * 2023-03-02 2026-02-27 アール．ピー． シェーラー テクノロジーズ エルエルシー 多能性幹細胞から間葉系間質細胞への分化の方法
CN121015869B (zh) * 2025-10-28 2026-03-17 深圳市中佳生物医疗科技有限公司 不同起始来源脐带血的cd73阳性nk细胞制备及阿兹海默症治疗的应用

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20090311735A1 (en) * 2006-07-06 2009-12-17 Es Cell International Pte Ltd Method for stem cell culture and cells derived therefrom
JP2014510537A (ja) * 2011-03-30 2014-05-01 セルラー ダイナミクス インターナショナル， インコーポレイテッド 神経分化のための多能性幹細胞の予備刺激
US20170369904A1 (en) * 2016-06-22 2017-12-28 Xcell Biosciences, Inc. Methods for increasing cell culture transfection efficiency and cellular reprogramming
WO2018067826A1 (en) * 2016-10-05 2018-04-12 Cellular Dynamics International, Inc. Generating mature lineages from induced pluripotent stem cells with mecp2 disruption

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP5733986B2 (ja) * 2008-02-21 2015-06-10 ヤンセン バイオテツク，インコーポレーテツド 細胞の付着、培養、及び剥離のための方法、表面改質されたプレート、並びに組成物
US9458431B2 (en) * 2008-03-17 2016-10-04 Agency For Science, Technology And Research Microcarriers for stem cell culture
KR101720961B1 (ko) * 2009-02-27 2017-03-29 셀룰러 다이내믹스 인터내셔널, 인코포레이티드 다능성 세포의 분화
JP6277187B2 (ja) * 2012-07-11 2018-02-07 イムステム バイオテクノロジー，インコーポレイテッド ヒト胚性幹細胞由来の間葉系様幹細胞、方法およびその使用
BR112017007770A2 (pt) * 2014-10-15 2018-01-16 Regeneron Pharma cultura in vitro, população de hipscs, método para modificar um locus-alvo genômico, e, hipsc.
US10947502B2 (en) * 2015-10-20 2021-03-16 FUJIFILM Cellular Dynamics, Inc. Methods for directed differentiation of pluripotent stem cells to immune cells
WO2017095333A1 (en) * 2015-11-30 2017-06-08 Agency For Science, Technology And Research A cell culture substrate and method of making thereof
WO2018037161A1 (en) * 2016-08-24 2018-03-01 Tampereen Yliopisto Differentiation of pluripotent stem cells into corneal cells
CN108949688B (zh) * 2018-08-07 2022-03-29 中山大学 一种来自多能干细胞的神经嵴谱系周细胞及其诱导分化方法

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20090311735A1 (en) * 2006-07-06 2009-12-17 Es Cell International Pte Ltd Method for stem cell culture and cells derived therefrom
JP2014510537A (ja) * 2011-03-30 2014-05-01 セルラー ダイナミクス インターナショナル， インコーポレイテッド 神経分化のための多能性幹細胞の予備刺激
US20170369904A1 (en) * 2016-06-22 2017-12-28 Xcell Biosciences, Inc. Methods for increasing cell culture transfection efficiency and cellular reprogramming
WO2018067826A1 (en) * 2016-10-05 2018-04-12 Cellular Dynamics International, Inc. Generating mature lineages from induced pluripotent stem cells with mecp2 disruption
JP2019528773A (ja) * 2016-10-05 2019-10-17 フジフィルム セルラー ダイナミクス，インコーポレイテッド ＭｅＣＰ２が破壊された誘導多能性幹細胞からの成熟系列の生成

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CA3143332A1 (en) 2020-12-17
AU2020293287A1 (en) 2022-01-06
KR20220032561A (ko) 2022-03-15
CN114174493B (zh) 2025-10-28
CN114174493A (zh) 2022-03-11
EP3983528A1 (en) 2022-04-20
WO2020252477A1 (en) 2020-12-17

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