JP2022535937A - レーザマイクロアレイチップスキャナの濃度勾配蛍光校正シート及び校正方法 - Google Patents
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Abstract
Description
[表1]実施形態における3つの群の走査パラメータの蛍光体ドット信号値
Claims (9)
- ガラス基板と前記ガラス基板の表面上に配置された無機ナノ蛍光液格子とを含み、
前記無機ナノ蛍光液格子が多数の無機ナノ蛍光液副格子を含み、各無機ナノ蛍光液副格子の無機蛍光混合液滴がA行×B列に配置され、前記無機ナノ蛍光液副格子内の同一行における無機ナノ蛍光液滴の溶液濃度が同一であり、2つの隣接する行の無機ナノ蛍光液滴の溶液濃度が傾斜的に倍数で増減し、Aが5-20、Bが10-40であることを特徴とするレーザマイクロアレイチップスキャナの濃度勾配蛍光校正シート。 - 前記ガラス基板がその表面上を化学的に修飾又はポリマー膜で被覆されていることを特徴とする請求項1に記載のレーザマイクロアレイチップスキャナの濃度勾配蛍光校正シート。
- 前記無機ナノ蛍光液格子が前記ガラス基板上に均一に分布及び配置された1-8個の無機ナノ蛍光液副格子を含むことを特徴とする請求項1に記載のレーザマイクロアレイチップスキャナの濃度勾配蛍光校正シート。
- 隣接する無機ナノ蛍光液副格子間のアレイ間隔が同一であり、隣接する無機ナノ蛍光液副格子間のドット間隔が同一であり、かつ350μm以上であることを特徴とする請求項1又は3に記載のレーザマイクロアレイチップスキャナの濃度勾配蛍光校正シート。
- 前記無機ナノ蛍光液副格子内の1行目の無機ナノ蛍光液滴及び最終行の無機ナノ蛍光液滴の溶液濃度が最大であり、2行目の無機ナノ蛍光液滴から、最後から2番目の無機ナノ蛍光液滴まで、溶液濃度が正の倍数で増加する線形関係を有し、同一の無機ナノ蛍光液副格子内の溶液の濃度勾配が12濃度勾配以下であることを特徴とする請求項1に記載のレーザマイクロアレイチップスキャナの濃度勾配蛍光校正シート。
- 1行目の無機ナノ蛍光液滴及び最終行の無機ナノ蛍光液滴の蛍光体ドット信号値が最大65535であり、2行目の無機ナノ蛍光液滴の蛍光体ドット信号値が1000~3000であり、最後から2番目の無機ナノ蛍光液滴の蛍光体ドット信号値が60000~65535であることを特徴とする請求項5に記載のレーザマイクロアレイチップスキャナの濃度勾配蛍光校正シート。
- 請求項1~6のいずれか1項に記載の前記レーザマイクロアレイチップスキャナの濃度勾配蛍光校正シートが被試験スキャナを校正するために利用され、前記無機ナノ蛍光液副格子内の1行目の無機ナノ蛍光液滴が指標マークとされることを特徴とするレーザマイクロアレイチップスキャナの校正方法。
- 前記無機ナノ蛍光液副格子内の各無機ナノ蛍光液滴における蛍光体ドット信号値が対応する溶液濃度の単一の測定値とされ、前記濃度勾配蛍光校正シートが標準スキャナで走査された後、対応する各無機ナノ蛍光液滴における蛍光体ドット信号値の対数値を横座標とし、又、前記濃度勾配蛍光校正シートが被試験スキャナで走査された後、対応する各無機ナノ蛍光液滴における蛍光体ドット信号値の対数値を縦座標とすることで図が作成され、調査を通して曲線間の線形関係の相関係数R2が得られることを特徴とする請求項7に記載のレーザマイクロアレイチップスキャナの校正方法。
- 各被試験スキャナが少なくとも3つの群の異なる走査パラメータによって校正され、各群の走査パラメータの相関係数R2が0.90より大きい場合、前記被試験スキャナが正常な校正結果を有し、良好な状態で使用できることを特徴とする請求項8に記載のレーザマイクロアレイチップスキャナの校正方法。
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CN201911148674.5A CN110865058A (zh) | 2019-11-21 | 2019-11-21 | 激光微阵列芯片扫描仪的浓度梯度荧光校准片及校准方法 |
PCT/CN2020/086375 WO2021098142A1 (zh) | 2019-11-21 | 2020-04-23 | 激光微阵列芯片扫描仪的浓度梯度荧光校准片及校准方法 |
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CN110865058A (zh) * | 2019-11-21 | 2020-03-06 | 成都博奥独立医学实验室有限公司 | 激光微阵列芯片扫描仪的浓度梯度荧光校准片及校准方法 |
CN111751339B (zh) * | 2020-06-23 | 2023-03-28 | 成都博奥晶芯生物科技有限公司 | 一种微阵列芯片激光共焦扫描仪的校准方法 |
CN112342276B (zh) * | 2020-11-09 | 2024-05-24 | 成都博奥独立医学实验室有限公司 | 核酸二级结构非特异荧光吸附用于微流控芯片检测的方法 |
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JP2019505824A (ja) * | 2015-12-23 | 2019-02-28 | コーニンクレッカ フィリップス エヌ ヴェKoninklijke Philips N.V. | 蛍光較正スライド |
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