JP2022534778A - 患者由来ミクロオルガノスフェアのための方法及び装置 - Google Patents

患者由来ミクロオルガノスフェアのための方法及び装置 Download PDF

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JP2022534778A
JP2022534778A JP2021571518A JP2021571518A JP2022534778A JP 2022534778 A JP2022534778 A JP 2022534778A JP 2021571518 A JP2021571518 A JP 2021571518A JP 2021571518 A JP2021571518 A JP 2021571518A JP 2022534778 A JP2022534778 A JP 2022534778A
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patient
microorganospheres
cells
derived
droplets
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JP2022534778A5 (https=
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シリン・シェン
ジャオウフイ・ワン
ダニエル・デルバック
ジェフリー・モッチマン
デイヴィッド・スー
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0693—Tumour cells; Cancer cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00—Bioreactors or fermenters specially adapted for specific uses
    • C12M21/08—Bioreactors or fermenters specially adapted for specific uses for producing artificial tissue or for ex-vivo cultivation of tissue
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
    • C12M25/01—Drops
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
    • C12M35/08—Chemical, biochemical or biological means, e.g. plasma jet, co-culture
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0012—Cell encapsulation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662—Stem cells
    • C12N5/0668—Mesenchymal stem cells from other natural sources
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/16—Microfluidic devices; Capillary tubes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
    • C12M25/16—Particles; Beads; Granular material; Encapsulation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/50—Soluble polymers, e.g. polyethyleneglycol [PEG]

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Sustainable Development (AREA)
  • Cell Biology (AREA)
  • Molecular Biology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Immunology (AREA)
  • Oncology (AREA)
  • Physiology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Rheumatology (AREA)
  • Dispersion Chemistry (AREA)
  • Clinical Laboratory Science (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
JP2021571518A 2019-05-28 2020-04-01 患者由来ミクロオルガノスフェアのための方法及び装置 Pending JP2022534778A (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201962853219P 2019-05-28 2019-05-28
US62/853,219 2019-05-28
PCT/US2020/026275 WO2020242594A1 (en) 2019-05-28 2020-04-01 Methods and apparatuses for patient-derived micro-organospheres

Publications (2)

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JP2022534778A true JP2022534778A (ja) 2022-08-03
JP2022534778A5 JP2022534778A5 (https=) 2023-04-07

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JP2021571518A Pending JP2022534778A (ja) 2019-05-28 2020-04-01 患者由来ミクロオルガノスフェアのための方法及び装置

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US (2) US11555180B2 (https=)
EP (1) EP3976064A4 (https=)
JP (1) JP2022534778A (https=)
CN (1) CN116157134A (https=)
AU (1) AU2020283390A1 (https=)
CA (1) CA3142113A1 (https=)
IL (1) IL288370A (https=)
MX (1) MX2021014439A (https=)
SG (1) SG11202113122SA (https=)
WO (1) WO2020242594A1 (https=)

Cited By (1)

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JP2023550651A (ja) * 2020-11-24 2023-12-04 デューク ユニバーシティ 液滴オルガノイドに基づく癌免疫学アッセイ及び其れを使用する方法
DE102022100146B4 (de) 2022-01-04 2023-11-30 Precomb Therapeutics Ag Verfahren zur analyse von personalisierten anti-krebs-wirkstoffen in zellkulturen
EP4514990A4 (en) * 2022-04-29 2026-05-06 Xilis Inc Methods and apparatuses for testing hepatocyte toxicity using microorganospheres
TW202407098A (zh) * 2022-05-03 2024-02-16 美商西利斯股份有限公司 衍生自患者之微器官球體(mos)實現臨床精準腫瘤學
JP2025528207A (ja) 2022-08-17 2025-08-26 ザイリス， インク． 画像に基づく微小有機球体薬剤アッセイ
CN120225691A (zh) * 2022-09-07 2025-06-27 西利斯公司 多发性骨髓瘤微器官球体
US11999934B2 (en) 2022-10-11 2024-06-04 Xilis, Inc. Integrated microfluidic system for generation of MicroOrganoSpheres (MOS)

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Publication number Publication date
EP3976064A4 (en) 2023-05-31
WO2020242594A1 (en) 2020-12-03
US20230002737A1 (en) 2023-01-05
EP3976064A1 (en) 2022-04-06
CA3142113A1 (en) 2020-12-03
MX2021014439A (es) 2022-05-19
US20200377861A1 (en) 2020-12-03
IL288370A (en) 2022-01-01
US11555180B2 (en) 2023-01-17
CN116157134A (zh) 2023-05-23
AU2020283390A1 (en) 2021-12-23
SG11202113122SA (en) 2021-12-30

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