JP2022530981A - プログラム可能なヌクレアーゼおよび使用方法 - Google Patents

プログラム可能なヌクレアーゼおよび使用方法 Download PDF

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JP2022530981A
JP2022530981A JP2021564788A JP2021564788A JP2022530981A JP 2022530981 A JP2022530981 A JP 2022530981A JP 2021564788 A JP2021564788 A JP 2021564788A JP 2021564788 A JP2021564788 A JP 2021564788A JP 2022530981 A JP2022530981 A JP 2022530981A
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nucleic acid
programmable
seq
target nucleic
nickase
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JP2022530981A5 (https=
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ルーカス ベンジャミン ハリントン
ジャニス シャ チェン
アイザック パターソン ウィッテ
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マンモス バイオサイエンシズ インコーポレイテッド
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/70—Carbohydrates; Sugars; Derivatives thereof
    • A61K31/7088—Compounds having three or more nucleosides or nucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
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  • Wood Science & Technology (AREA)
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  • Public Health (AREA)
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  • Mycology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
JP2021564788A 2019-05-01 2020-05-01 プログラム可能なヌクレアーゼおよび使用方法 Pending JP2022530981A (ja)

Applications Claiming Priority (3)

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US201962841770P 2019-05-01 2019-05-01
US62/841,770 2019-05-01
PCT/US2020/031030 WO2020223634A1 (en) 2019-05-01 2020-05-01 Programmable nucleases and methods of use

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JP2022530981A true JP2022530981A (ja) 2022-07-05
JP2022530981A5 JP2022530981A5 (https=) 2023-05-10

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JP2021564788A Pending JP2022530981A (ja) 2019-05-01 2020-05-01 プログラム可能なヌクレアーゼおよび使用方法

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US (2) US20220049241A1 (https=)
EP (1) EP3963069A1 (https=)
JP (1) JP2022530981A (https=)
CN (1) CN114174508A (https=)
AU (1) AU2020265256A1 (https=)
CA (1) CA3134934A1 (https=)
MX (1) MX2021013302A (https=)
WO (1) WO2020223634A1 (https=)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2020117715A1 (en) 2018-12-03 2020-06-11 Board Of Regents, The University Of Texas System Oligo-benzamide analogs and their use in cancer treatment
CA3192654A1 (en) 2020-09-01 2022-03-10 The Board Of Trustees Of The Leland Stanford Junior Unversity Synthetic miniature crispr-cas (casmini) system for eukaryotic genome engineering
WO2022241032A1 (en) * 2021-05-12 2022-11-17 Mammoth Biosciences, Inc. Enhanced guide nucleic acids and methods of use
CA3220294A1 (en) * 2021-05-14 2022-11-17 Genkore Inc. Composition and method for treatment of lca10 using rna-guided nuclease
EP4347818A2 (en) 2021-06-01 2024-04-10 Arbor Biotechnologies, Inc. Gene editing systems comprising a crispr nuclease and uses thereof
WO2023283622A1 (en) 2021-07-08 2023-01-12 Montana State University Crispr-based programmable rna editing
WO2023004391A2 (en) 2021-07-21 2023-01-26 Montana State University Nucleic acid detection using type iii crispr complex
CN119137268A (zh) 2022-03-01 2024-12-13 埃皮克里斯皮尔生物技术公司 工程化核酸酶、组合物及其使用方法
KR20250151430A (ko) 2023-02-15 2025-10-21 아버 바이오테크놀로지스, 인크. 스타스민 2(stmn2) 전사체에서 비정상적인 스플라이싱을 억제하는 유전자 편집 방법
WO2025171210A1 (en) 2024-02-09 2025-08-14 Arbor Biotechnologies, Inc. Compositions and methods for gene editing via homology-mediated end joining
WO2026015832A2 (en) 2024-07-12 2026-01-15 Arbor Biotechnologies, Inc. Reverse transcriptases and gene editing systems comprising such
WO2026015829A2 (en) 2024-07-12 2026-01-15 Arbor Biotechnologies, Inc. Small reverse transcriptases and gene editing systems comprising such

Family Cites Families (1)

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Publication number Priority date Publication date Assignee Title
AU2018358051B2 (en) * 2017-11-01 2025-01-09 The Regents Of The University Of California CasZ compositions and methods of use

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
MOLECULAR CELL, vol. 60(3), JPN6024021869, 2015, pages 385 - 397, ISSN: 0005471738 *
MOLECULAR CELL, vol. 65(2), JPN6024021872, 2017, pages 310 - 322, ISSN: 0005471741 *
NATURE REVIEWS. MICROBIOLOGY, vol. 15(3), JPN6024021871, 2017, pages 169 - 182, ISSN: 0005471740 *
SCIENCE, vol. 362(6416), JPN6024021868, 2018, pages 839 - 842, ISSN: 0005471737 *
SCIENCE, vol. 363(6422), JPN6024021870, 4 January 2019 (2019-01-04), pages 88 - 91, ISSN: 0005471739 *

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Publication number Publication date
WO2020223634A1 (en) 2020-11-05
CN114174508A (zh) 2022-03-11
CA3134934A1 (en) 2020-11-05
US20240191224A1 (en) 2024-06-13
MX2021013302A (es) 2022-01-31
AU2020265256A1 (en) 2021-12-23
US20220049241A1 (en) 2022-02-17
EP3963069A1 (en) 2022-03-09

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