CN114174508A - 可编程核酸酶和使用方法 - Google Patents

可编程核酸酶和使用方法 Download PDF

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Publication number
CN114174508A
CN114174508A CN202080048714.3A CN202080048714A CN114174508A CN 114174508 A CN114174508 A CN 114174508A CN 202080048714 A CN202080048714 A CN 202080048714A CN 114174508 A CN114174508 A CN 114174508A
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China
Prior art keywords
nucleic acid
programmable
fold
seq
target nucleic
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CN202080048714.3A
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English (en)
Chinese (zh)
Inventor
卢卡斯·本杰明·哈林顿
贾尼斯·沙·陈
伊萨克·帕特森·维特
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Mammoth Biosciences Inc
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Mammoth Biosciences Inc
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Publication of CN114174508A publication Critical patent/CN114174508A/zh
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/70—Carbohydrates; Sugars; Derivatives thereof
    • A61K31/7088—Compounds having three or more nucleosides or nucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Mycology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
CN202080048714.3A 2019-05-01 2020-05-01 可编程核酸酶和使用方法 Pending CN114174508A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201962841770P 2019-05-01 2019-05-01
US62/841,770 2019-05-01
PCT/US2020/031030 WO2020223634A1 (en) 2019-05-01 2020-05-01 Programmable nucleases and methods of use

Publications (1)

Publication Number Publication Date
CN114174508A true CN114174508A (zh) 2022-03-11

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
CN202080048714.3A Pending CN114174508A (zh) 2019-05-01 2020-05-01 可编程核酸酶和使用方法

Country Status (8)

Country Link
US (2) US20220049241A1 (https=)
EP (1) EP3963069A1 (https=)
JP (1) JP2022530981A (https=)
CN (1) CN114174508A (https=)
AU (1) AU2020265256A1 (https=)
CA (1) CA3134934A1 (https=)
MX (1) MX2021013302A (https=)
WO (1) WO2020223634A1 (https=)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2020117715A1 (en) 2018-12-03 2020-06-11 Board Of Regents, The University Of Texas System Oligo-benzamide analogs and their use in cancer treatment
CA3192654A1 (en) 2020-09-01 2022-03-10 The Board Of Trustees Of The Leland Stanford Junior Unversity Synthetic miniature crispr-cas (casmini) system for eukaryotic genome engineering
WO2022241032A1 (en) * 2021-05-12 2022-11-17 Mammoth Biosciences, Inc. Enhanced guide nucleic acids and methods of use
CA3220294A1 (en) * 2021-05-14 2022-11-17 Genkore Inc. Composition and method for treatment of lca10 using rna-guided nuclease
EP4347818A2 (en) 2021-06-01 2024-04-10 Arbor Biotechnologies, Inc. Gene editing systems comprising a crispr nuclease and uses thereof
WO2023283622A1 (en) 2021-07-08 2023-01-12 Montana State University Crispr-based programmable rna editing
WO2023004391A2 (en) 2021-07-21 2023-01-26 Montana State University Nucleic acid detection using type iii crispr complex
CN119137268A (zh) 2022-03-01 2024-12-13 埃皮克里斯皮尔生物技术公司 工程化核酸酶、组合物及其使用方法
KR20250151430A (ko) 2023-02-15 2025-10-21 아버 바이오테크놀로지스, 인크. 스타스민 2(stmn2) 전사체에서 비정상적인 스플라이싱을 억제하는 유전자 편집 방법
WO2025171210A1 (en) 2024-02-09 2025-08-14 Arbor Biotechnologies, Inc. Compositions and methods for gene editing via homology-mediated end joining
WO2026015832A2 (en) 2024-07-12 2026-01-15 Arbor Biotechnologies, Inc. Reverse transcriptases and gene editing systems comprising such
WO2026015829A2 (en) 2024-07-12 2026-01-15 Arbor Biotechnologies, Inc. Small reverse transcriptases and gene editing systems comprising such

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2019089820A1 (en) * 2017-11-01 2019-05-09 The Regents Of The University Of California Casz compositions and methods of use

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2019089820A1 (en) * 2017-11-01 2019-05-09 The Regents Of The University Of California Casz compositions and methods of use

Non-Patent Citations (9)

* Cited by examiner, † Cited by third party
Title
HARRINGTON LB 等: "Programmed DNA destruction by miniature CRISPR-Cas14 enzymes", 《SCIENCE》, vol. 362, no. 6416, 16 November 2018 (2018-11-16), pages 839 - 842, XP055614750, DOI: 10.1126/science.aav4294 *
HARRINGTON LB等: "Programmed DNA destruction by miniature CRISPR-Cas14 enzymes", SCIENCE, vol. 362, no. 6416, 16 November 2018 (2018-11-16), pages 839 - 842, XP055614750, DOI: 10.1126/science.aav4294 *
JIANKUI ZHOU 等: "Dual sgRNAs facilitate CRISPR/Cas9-mediated mouse genome targeting", 《FEBS JOURNAL》, vol. 281, 31 December 2014 (2014-12-31), pages 1718 *
KARVELIS T等: "PAM recognition by miniature CRISPR–Cas12f nucleases triggers programmable double-stranded DNA target cleavage", NUCLEIC ACIDS RESEARCH, vol. 48, no. 9, 31 December 2020 (2020-12-31), pages 5016 - 5023, XP055920188, DOI: 10.1093/nar/gkaa208 *
LINO C A等: "Delivering CRISPR: a review of the challenges and approaches", DRUG DELIVERY, vol. 25, no. 1, 31 December 2018 (2018-12-31), pages 1234 - 1257, XP055573342, DOI: 10.1080/10717544.2018.1474964 *
LIU L等: "C2c1-sgRNA complex structure reveals RNA-guided DNA cleavage mechanism", MOLECULAR CELL, vol. 65, no. 2, 31 December 2017 (2017-12-31), pages 310 - 322, XP029890333, DOI: 10.1016/j.molcel.2016.11.040 *
SERGEY SHMAKOV等: "Discovery and Functional Characterization of Diverse Class 2 CRISPR-Cas Systems", MOLECULAR CELL, vol. 60, no. 3, 31 December 2015 (2015-12-31), pages 385 - 397, XP055785070, DOI: 10.1016/j.molcel.2015.10.008 *
SHMAKOV S等: "Diversity and evolution of class 2 CRISPR–Cas systems", NATURE REVIEWS MICROBIOLOGY, vol. 15, no. 3, 31 December 2017 (2017-12-31), pages 169 - 182, XP093011863, DOI: 10.1038/nrmicro.2016.184 *
YAN WX等: "Functionally diverse type V CRISPR-Cas systems", SCIENCE, vol. 363, no. 6422, 4 January 2019 (2019-01-04), pages 88 - 91, XP055594948, DOI: 10.1126/science.aav7271 *

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Publication number Publication date
WO2020223634A1 (en) 2020-11-05
JP2022530981A (ja) 2022-07-05
CA3134934A1 (en) 2020-11-05
US20240191224A1 (en) 2024-06-13
MX2021013302A (es) 2022-01-31
AU2020265256A1 (en) 2021-12-23
US20220049241A1 (en) 2022-02-17
EP3963069A1 (en) 2022-03-09

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Application publication date: 20220311