JP2022516684A - ヌクレオチドの開裂可能なリンカーおよびその使用 - Google Patents

ヌクレオチドの開裂可能なリンカーおよびその使用 Download PDF

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JP2022516684A
JP2022516684A JP2021562929A JP2021562929A JP2022516684A JP 2022516684 A JP2022516684 A JP 2022516684A JP 2021562929 A JP2021562929 A JP 2021562929A JP 2021562929 A JP2021562929 A JP 2021562929A JP 2022516684 A JP2022516684 A JP 2022516684A
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JP2022516684A5 (enExample
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グレアム，ロナルド
アデルフィンスカヤ，オルガ
シーラ，メガ
ロドリゲス，ロドリゴ
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シンギュラー・ゲノミクス・システムズ・インコーポレイテッド
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    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
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    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/06—Pyrimidine radicals
    • C07H19/10—Pyrimidine radicals with the saccharide radical esterified by phosphoric or polyphosphoric acids
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    • C07F9/00—Compounds containing elements of Groups 5 or 15 of the Periodic Table
    • C07F9/02—Phosphorus compounds
    • C07F9/547—Heterocyclic compounds, e.g. containing phosphorus as a ring hetero atom
    • C07F9/655—Heterocyclic compounds, e.g. containing phosphorus as a ring hetero atom having oxygen atoms, with or without sulfur, selenium, or tellurium atoms, as the only ring hetero atoms
    • C07F9/65515—Heterocyclic compounds, e.g. containing phosphorus as a ring hetero atom having oxygen atoms, with or without sulfur, selenium, or tellurium atoms, as the only ring hetero atoms the oxygen atom being part of a five-membered ring
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    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/06—Pyrimidine radicals
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    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/16—Purine radicals
    • C—CHEMISTRY; METALLURGY
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    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/16—Purine radicals
    • C07H19/20—Purine radicals with the saccharide radical esterified by phosphoric or polyphosphoric acids
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
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    • C12Q2334/00—O-linked chromogens for determinations of hydrolase enzymes, e.g. glycosidases, phosphatases, esterases
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    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/10—Nucleotidyl transfering
    • C12Q2521/101—DNA polymerase
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    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/101—Modifications characterised by incorporating non-naturally occurring nucleotides, e.g. inosine
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    • C12Q2535/00—Reactions characterised by the assay type for determining the identity of a nucleotide base or a sequence of oligonucleotides
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/50—Improvements relating to the production of bulk chemicals
    • Y02P20/55—Design of synthesis routes, e.g. reducing the use of auxiliary or protecting groups

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  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Plural Heterocyclic Compounds (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
JP2021562929A 2019-01-08 2020-01-07 ヌクレオチドの開裂可能なリンカーおよびその使用 Pending JP2022516684A (ja)

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US201962789879P 2019-01-08 2019-01-08
US62/789,879 2019-01-08
US201962841168P 2019-04-30 2019-04-30
US62/841,168 2019-04-30
PCT/US2020/012595 WO2020146397A1 (en) 2019-01-08 2020-01-07 Nucleotide cleavable linkers and uses thereof

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JP2022516684A5 JP2022516684A5 (enExample) 2022-10-17

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US (4) US11970735B2 (enExample)
EP (1) EP3908286A4 (enExample)
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WO (1) WO2020146397A1 (enExample)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3596099B1 (en) 2017-03-06 2024-07-24 Singular Genomics Systems, Inc. Nucleic acid sequencing-by-synthesis (sbs) methods that combine sbs cycle steps
US12215124B2 (en) * 2018-02-21 2025-02-04 Singular Genomics Systems, Inc. Fluorinated nucleotides and uses thereof
WO2019222653A1 (en) 2018-05-18 2019-11-21 Singular Genomics Systems, Inc. Silicon containing detectable compounds
CN113039191B (zh) 2018-10-25 2024-07-16 奇异基因组学系统公司 核苷酸类似物
JP2022516684A (ja) 2019-01-08 2022-03-01 シンギュラー・ゲノミクス・システムズ・インコーポレイテッド ヌクレオチドの開裂可能なリンカーおよびその使用
EP4055023A4 (en) 2019-11-07 2024-01-03 Singular Genomics Systems, Inc. SILICON-CONTAINING DETECTED COMPOUNDS AND USES THEREOF
US11174281B1 (en) 2020-04-24 2021-11-16 Singular Genomics Systems, Inc. Modified nucleotides and uses thereof
WO2022094415A1 (en) 2020-10-30 2022-05-05 Singular Genomics Systems, Inc. Methods and compositions for reducing nucleotide impurities
CA3214986A1 (en) * 2021-04-01 2022-10-06 Bgi Shenzhen Nucleotide analogue for sequencing
WO2023107673A2 (en) 2021-12-10 2023-06-15 Singular Genomics Systems, Inc. Cleavable disulfide linkers and uses thereof
JP2025505253A (ja) * 2022-02-11 2025-02-21 ミルテニー バイオテック ベー．フェー． ウント コー． カー・ゲー 酵素的ｄｎａおよびｒｎａ合成のための３’－オキシメチレンアルキルジスルフィド保護ヌクレオチドの使用
US12359068B2 (en) 2023-02-10 2025-07-15 Singular Genomics Systems, Inc. Rhodamine fluorescent compounds and production methods thereof

Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2005511058A (ja) * 2001-12-04 2005-04-28 ソレックサ リミテッド 標識ヌクレオチド
JP2006509040A (ja) * 2002-08-23 2006-03-16 ソレックサ リミテッド 修飾されたヌクレオチド
WO2009051807A1 (en) * 2007-10-19 2009-04-23 The Trustees Of Columbia University In The City Of New York Design and synthesis of cleavable fluorescent nucleotides as reversible terminators for dna sequencing by synthesis
WO2016182984A1 (en) * 2015-05-08 2016-11-17 Centrillion Technology Holdings Corporation Disulfide-linked reversible terminators
WO2017058953A1 (en) * 2015-09-28 2017-04-06 The Trustees Of Columbia University In The City Of New York Design and synthesis of novel disulfide linker based nucleotides as reversible terminators for dna sequencing by synthesis
WO2017079498A2 (en) * 2015-11-06 2017-05-11 Intelligent Biosystems, Inc. Nucleotide analogues
JP2019536448A (ja) * 2016-11-04 2019-12-19 キアゲン サイエンシーズ， エルエルシー チオール含有切断試薬および酸化的洗浄液
WO2020086834A1 (en) * 2018-10-25 2020-04-30 Singular Genomics Systems, Inc. Nucleotide analogues

Family Cites Families (64)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5936696A (ja) 1982-08-20 1984-02-28 Taiho Yakuhin Kogyo Kk ２′―デオキシ―５―置換ウリジン誘導体及びそれを含有する抗腫瘍剤
US5235033A (en) 1985-03-15 1993-08-10 Anti-Gene Development Group Alpha-morpholino ribonucleoside derivatives and polymers thereof
US5034506A (en) 1985-03-15 1991-07-23 Anti-Gene Development Group Uncharged morpholino-based polymers having achiral intersubunit linkages
US5804386A (en) 1997-01-15 1998-09-08 Incyte Pharmaceuticals, Inc. Sets of labeled energy transfer fluorescent primers and their use in multi component analysis
US5876936A (en) 1997-01-15 1999-03-02 Incyte Pharmaceuticals, Inc. Nucleic acid sequencing with solid phase capturable terminators
US6046005A (en) 1997-01-15 2000-04-04 Incyte Pharmaceuticals, Inc. Nucleic acid sequencing with solid phase capturable terminators comprising a cleavable linking group
US6485944B1 (en) 1997-10-10 2002-11-26 President And Fellows Of Harvard College Replica amplification of nucleic acid arrays
US6309836B1 (en) 1999-10-05 2001-10-30 Marek Kwiatkowski Compounds for protecting hydroxyls and methods for their use
US20060057565A1 (en) 2000-09-11 2006-03-16 Jingyue Ju Combinatorial fluorescence energy transfer tags and uses thereof
US6627748B1 (en) 2000-09-11 2003-09-30 The Trustees Of Columbia University In The City Of New York Combinatorial fluorescence energy transfer tags and their applications for multiplex genetic analyses
CA2421582A1 (en) 2000-09-11 2002-03-21 The Trustees Of Columbia University In The City Of New York Combinatorial fluorescence energy transfer tags and uses thereof
AU2001296645A1 (en) 2000-10-06 2002-04-15 The Trustees Of Columbia University In The City Of New York Massive parallel method for decoding dna and rna
US9708358B2 (en) 2000-10-06 2017-07-18 The Trustees Of Columbia University In The City Of New York Massive parallel method for decoding DNA and RNA
US20030027140A1 (en) 2001-03-30 2003-02-06 Jingyue Ju High-fidelity DNA sequencing using solid phase capturable dideoxynucleotides and mass spectrometry
GB0129012D0 (en) 2001-12-04 2002-01-23 Solexa Ltd Labelled nucleotides
US7074597B2 (en) 2002-07-12 2006-07-11 The Trustees Of Columbia University In The City Of New York Multiplex genotyping using solid phase capturable dideoxynucleotides and mass spectrometry
US20040022764A1 (en) 2002-07-31 2004-02-05 Hanan Polansky Inhibition of microcompetition with a foreign polynucleotide as treatment of chronic disease
US7414116B2 (en) 2002-08-23 2008-08-19 Illumina Cambridge Limited Labelled nucleotides
GB0324456D0 (en) 2003-10-20 2003-11-19 Isis Innovation Parallel DNA sequencing methods
EP2436778A3 (en) 2004-03-03 2012-07-11 The Trustees of Columbia University in the City of New York Photocleavable fluorescent nucleotides for DNA sequencing on chip constructed by site-specific coupling chemistry
US20080103053A1 (en) 2005-11-22 2008-05-01 Helicos Biosciences Corporation Methods and compositions for sequencing a nucleic acid
US8114973B2 (en) 2004-05-25 2012-02-14 Helicos Biosciences Corporation Nucleotide analogs
US20070009980A1 (en) 2004-06-01 2007-01-11 Applera Corporation Continuous fluorogenic analyte assays with dendritic amplification of signal
US20060003383A1 (en) 2004-06-07 2006-01-05 Applera Corporation Fluorogenic enzyme activity assay methods and compositions using fragmentable linkers
US9169510B2 (en) 2005-06-21 2015-10-27 The Trustees Of Columbia University In The City Of New York Pyrosequencing methods and related compositions
GB2446083B (en) 2005-10-31 2011-03-02 Univ Columbia Chemically cleavable 3'-0-allyl-dntp-allyl-fluorophore fluorescent nucleotide analogues and related methods
US7982029B2 (en) 2005-10-31 2011-07-19 The Trustees Of Columbia University In The City Of New York Synthesis of four color 3′O-allyl, modified photocleavable fluorescent nucleotides and related methods
WO2007146158A1 (en) 2006-06-07 2007-12-21 The Trustees Of Columbia University In The City Of New York Dna sequencing by nanopore using modified nucleotides
WO2008037568A2 (en) 2006-09-04 2008-04-03 Quiatech Ab Reversible terminators for efficient sequencing by synthesis
WO2008042067A2 (en) 2006-09-28 2008-04-10 Illumina, Inc. Compositions and methods for nucleotide sequencing
GB2457402B (en) 2006-12-01 2011-10-19 Univ Columbia Four-color DNA sequencing by synthesis using cleavable fluorescent nucleotide reversible terminators
US8481259B2 (en) 2007-02-05 2013-07-09 Intelligent Bio-Systems, Inc. Methods and devices for sequencing nucleic acids in smaller batches
DK2725107T3 (da) 2007-10-19 2019-01-02 Univ Columbia DNA-sekventering med ikke-fluorescerende nukleotidreversible terminatorer og ddNTP'er modificeret med spaltbart mærke og nukleinsyre omfattende inosin med reversible terminatorer
US9017973B2 (en) 2008-03-19 2015-04-28 Intelligent Biosystems, Inc. Methods and compositions for incorporating nucleotides
US8772473B2 (en) 2009-03-30 2014-07-08 The Regents Of The University Of California Mostly natural DNA sequencing by synthesis
WO2012083249A2 (en) 2010-12-17 2012-06-21 The Trustees Of Columbia University In The City Of New York Dna sequencing by synthesis using modified nucleotides and nanopore detection
US9624539B2 (en) 2011-05-23 2017-04-18 The Trustees Of Columbia University In The City Of New York DNA sequencing by synthesis using Raman and infrared spectroscopy detection
WO2013040257A1 (en) 2011-09-13 2013-03-21 Lasergen, Inc. 5-methoxy. 3'-oh unblocked, fast photocleavable terminating nucleotides and methods for nucleic acid sequencing
WO2013154999A2 (en) 2012-04-09 2013-10-17 The Trustees Of Columbia University In The City Of New York Method of preparation of nanopore and uses thereof
EP3674412A1 (en) 2012-06-20 2020-07-01 The Trustees of Columbia University in the City of New York Nucleic acid sequencing by nanopore detection of tag molecules
US10732183B2 (en) 2013-03-15 2020-08-04 The Trustees Of Columbia University In The City Of New York Method for detecting multiple predetermined compounds in a sample
WO2014144883A1 (en) 2013-03-15 2014-09-18 The Trustees Of Columbia University In The City Of New York Raman cluster tagged molecules for biological imaging
WO2014139596A1 (en) 2013-03-15 2014-09-18 Illumina Cambridge Limited Modified nucleosides or nucleotides
EP2876166B1 (en) 2013-11-20 2016-12-14 Roche Diagnostics GmbH New compound for sequencing by synthesis
US10718011B2 (en) 2014-02-12 2020-07-21 The Trustees Of Columbia University In The City Of New York Single molecule electronic multiplex SNP assay and PCR analysis
MA39774A (fr) 2014-03-24 2021-05-12 Roche Sequencing Solutions Inc Procédés chimiques pour produire des nucléotides étiquetés
GB201414098D0 (en) 2014-08-08 2014-09-24 Illumina Cambridge Ltd Modified nucleotide linkers
EP3699283A1 (en) 2014-10-20 2020-08-26 Molecular Assemblies Inc. Modified template-independent enzymes for polydeoxynucleotide systhesis
GB201418718D0 (en) 2014-10-21 2014-12-03 Cambridge Epigenetix Ltd Improved nucleic acid re-sequencing using a reduced number of identified bases
WO2016144973A1 (en) 2015-03-09 2016-09-15 The Trustees Of Columbia University In The City Of New York Pore-forming protein conjugate compositions and methods
CN107922968B (zh) 2015-03-23 2022-05-17 哥伦比亚大学董事会 用于单分子电子snp测定的聚合物标记的核苷酸
US11421264B2 (en) 2015-11-06 2022-08-23 IsoPlexis Corporation Thiol-containing cleave reagents and oxidative wash
WO2017087887A1 (en) 2015-11-18 2017-05-26 The Trustees Of Columbia University In The City Of New York Ion sensor dna and rna sequencing by synthesis using nucleotide reversible terminators
US11384391B2 (en) 2016-04-04 2022-07-12 The Trustees Of Columbia University In The City Of New York Flourescene energy transfer-based single molecule/ensemble DNA sequencing by synthesis
EP3458082A4 (en) 2016-04-04 2020-07-29 The Trustees of Columbia University in the City of New York SYNTHESIS DNA SEQUENCING WITH NUCLEOTID ANALOGUES AND RAMAN DETECTION
WO2017205336A1 (en) 2016-05-23 2017-11-30 The Trustees Of Columbia University In The City Of New York Nucleotide derivatives and methods of use thereof
EP3596099B1 (en) 2017-03-06 2024-07-24 Singular Genomics Systems, Inc. Nucleic acid sequencing-by-synthesis (sbs) methods that combine sbs cycle steps
WO2018183538A1 (en) 2017-03-28 2018-10-04 The Trustees Of Columbia University In The City Of New York 3'-o-modified nucleotide analogues with different cleavable linkers for attaching fluorescent labels to the base for dna sequencing by synthesis
US20190077726A1 (en) 2017-09-13 2019-03-14 Singular Genomics Systems, Inc. Methods of synthesizing labeled nucleosides
WO2019071474A1 (zh) 2017-10-11 2019-04-18 深圳华大智造科技有限公司 修饰的核苷或核苷酸
EP3733683A4 (en) 2017-11-30 2022-04-06 GeneMind Biosciences Company Limited NUCLEOSIDE ANALOGUES, METHOD OF MANUFACTURE AND USE
US12215124B2 (en) 2018-02-21 2025-02-04 Singular Genomics Systems, Inc. Fluorinated nucleotides and uses thereof
JP2022516684A (ja) 2019-01-08 2022-03-01 シンギュラー・ゲノミクス・システムズ・インコーポレイテッド ヌクレオチドの開裂可能なリンカーおよびその使用
WO2020146497A1 (en) 2019-01-09 2020-07-16 The Regents Of The University Of California An electrochemiluminescence elisa for ps129-a-synuclein

Patent Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2005511058A (ja) * 2001-12-04 2005-04-28 ソレックサ リミテッド 標識ヌクレオチド
JP2006509040A (ja) * 2002-08-23 2006-03-16 ソレックサ リミテッド 修飾されたヌクレオチド
WO2009051807A1 (en) * 2007-10-19 2009-04-23 The Trustees Of Columbia University In The City Of New York Design and synthesis of cleavable fluorescent nucleotides as reversible terminators for dna sequencing by synthesis
WO2016182984A1 (en) * 2015-05-08 2016-11-17 Centrillion Technology Holdings Corporation Disulfide-linked reversible terminators
WO2017058953A1 (en) * 2015-09-28 2017-04-06 The Trustees Of Columbia University In The City Of New York Design and synthesis of novel disulfide linker based nucleotides as reversible terminators for dna sequencing by synthesis
WO2017079498A2 (en) * 2015-11-06 2017-05-11 Intelligent Biosystems, Inc. Nucleotide analogues
JP2019536448A (ja) * 2016-11-04 2019-12-19 キアゲン サイエンシーズ， エルエルシー チオール含有切断試薬および酸化的洗浄液
WO2020086834A1 (en) * 2018-10-25 2020-04-30 Singular Genomics Systems, Inc. Nucleotide analogues

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
ANALYTICAL BIOCHEMISTRY, vol. 330, JPN6023038600, 2004, pages 206 - 218, ISSN: 0005152925 *

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