JP2022121814A - algae inhibitor - Google Patents
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- JP2022121814A JP2022121814A JP2021018725A JP2021018725A JP2022121814A JP 2022121814 A JP2022121814 A JP 2022121814A JP 2021018725 A JP2021018725 A JP 2021018725A JP 2021018725 A JP2021018725 A JP 2021018725A JP 2022121814 A JP2022121814 A JP 2022121814A
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- 241000195493 Cryptophyta Species 0.000 title claims abstract description 63
- 239000003112 inhibitor Substances 0.000 title claims abstract description 25
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 24
- 108010034594 hydralysin Proteins 0.000 claims description 38
- 238000009395 breeding Methods 0.000 claims description 10
- 230000001488 breeding effect Effects 0.000 claims description 10
- 238000000034 method Methods 0.000 claims description 10
- 239000007787 solid Substances 0.000 claims description 8
- 230000000694 effects Effects 0.000 abstract description 24
- 239000004472 Lysine Substances 0.000 abstract 2
- 241000195628 Chlorophyta Species 0.000 description 11
- 241000206761 Bacillariophyta Species 0.000 description 9
- 241000588724 Escherichia coli Species 0.000 description 9
- 241000243252 Hydra viridissima Species 0.000 description 8
- 108090000623 proteins and genes Proteins 0.000 description 7
- 241000243251 Hydra Species 0.000 description 6
- 239000003795 chemical substances by application Substances 0.000 description 6
- QRXWMOHMRWLFEY-UHFFFAOYSA-N isoniazide Chemical compound NNC(=O)C1=CC=NC=C1 QRXWMOHMRWLFEY-UHFFFAOYSA-N 0.000 description 6
- 102000004169 proteins and genes Human genes 0.000 description 5
- 239000000126 substance Substances 0.000 description 5
- 239000000654 additive Substances 0.000 description 4
- 230000002401 inhibitory effect Effects 0.000 description 4
- 239000000463 material Substances 0.000 description 4
- 230000000996 additive effect Effects 0.000 description 3
- 230000002411 adverse Effects 0.000 description 3
- 238000012258 culturing Methods 0.000 description 3
- 230000007423 decrease Effects 0.000 description 3
- 229910052500 inorganic mineral Inorganic materials 0.000 description 3
- 239000007788 liquid Substances 0.000 description 3
- 239000011707 mineral Substances 0.000 description 3
- 235000015097 nutrients Nutrition 0.000 description 3
- 238000005215 recombination Methods 0.000 description 3
- 230000028327 secretion Effects 0.000 description 3
- 241000251468 Actinopterygii Species 0.000 description 2
- 230000002353 algacidal effect Effects 0.000 description 2
- 230000000844 anti-bacterial effect Effects 0.000 description 2
- 230000000052 comparative effect Effects 0.000 description 2
- 229910021645 metal ion Inorganic materials 0.000 description 2
- 239000000843 powder Substances 0.000 description 2
- 230000006798 recombination Effects 0.000 description 2
- 206010000117 Abnormal behaviour Diseases 0.000 description 1
- 241000023795 Achnanthidium minutissimum Species 0.000 description 1
- 241000195641 Characium Species 0.000 description 1
- 241000243321 Cnidaria Species 0.000 description 1
- 241000238424 Crustacea Species 0.000 description 1
- JPVYNHNXODAKFH-UHFFFAOYSA-N Cu2+ Chemical compound [Cu+2] JPVYNHNXODAKFH-UHFFFAOYSA-N 0.000 description 1
- 241000192700 Cyanobacteria Species 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 241000947662 Fontinalis antipyretica Species 0.000 description 1
- 241000243328 Hydridae Species 0.000 description 1
- 125000002059 L-arginyl group Chemical group O=C([*])[C@](N([H])[H])([H])C([H])([H])C([H])([H])C([H])([H])N([H])C(=N[H])N([H])[H] 0.000 description 1
- 239000004480 active ingredient Substances 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 241001233037 catfish Species 0.000 description 1
- 229910001431 copper ion Inorganic materials 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 238000007667 floating Methods 0.000 description 1
- 239000013505 freshwater Substances 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 230000002147 killing effect Effects 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 239000008188 pellet Substances 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 230000002829 reductive effect Effects 0.000 description 1
- 239000004576 sand Substances 0.000 description 1
- 238000007790 scraping Methods 0.000 description 1
- 229910052709 silver Inorganic materials 0.000 description 1
- 239000004332 silver Substances 0.000 description 1
- -1 silver ions Chemical class 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 239000007858 starting material Substances 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 230000009261 transgenic effect Effects 0.000 description 1
Classifications
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Abstract
Description
本発明は、水棲生物の飼育水槽の壁面や水槽内アクセサリーに付着する藻類の繁茂を抑制する藻類抑制剤およびその関連技術に関する。 TECHNICAL FIELD The present invention relates to an algae inhibitor for suppressing growth of algae adhering to the walls of an aquarium for breeding aquatic organisms and accessories in the aquarium, and related arts thereof.
鑑賞用水棲生物を飼育する水槽内に珪藻、緑藻、藍藻等の藻類が発生すると、水槽の壁面や石・流木等のアクセサリーに付着し、あるいは水を濁らせて、景観を損ねる。 When algae such as diatoms, green algae and blue-green algae grow in aquariums for breeding ornamental aquatic organisms, they adhere to the walls of the aquarium and accessories such as stones and driftwood, or muddy the water, thereby spoiling the scenery.
水槽内の藻類除去方法としては、付着した藻類をこそげ落とす他に、殺藻物質や、付着藻類の養分となる物質を除去する添加剤を水槽の水に入れる方法がある。殺藻物質の添加剤としては、銅イオンや銀イオンなどの殺菌効果のある物質を応用したものが多く、付着藻類自体を死滅させるために防藻効果が得られやすい（非特許文献１参照）。 As a method for removing algae in a tank, there is a method of adding an algicidal substance or an additive for removing substances that serve as nutrients for the attached algae to the water of the tank, in addition to scraping off the adhering algae. Algaecidal additives often use substances with a bactericidal effect, such as copper ions and silver ions, and are likely to have an anti-algae effect because they kill the adhering algae themselves (see Non-Patent Document 1). .
また、本出願人は、水棲生物をグリーンヒドラとともに飼育して藻類を除去する方法を提案した（特許文献１）。 The present applicant also proposed a method of breeding aquatic organisms together with Green Hydra to remove algae (Patent Document 1).
しかし、金属イオンのような殺菌効果を有する物質は、濃度が高いと飼育する魚類や甲殻類、植物などの死滅や異常行動を招くことがある。また、濃度にかかわらず、特定の飼育生物種、例えばナマズ類、古代魚、ウイローモス等に対して毒性が認められる物質もある。 However, if the concentration of a substance with a bactericidal effect such as a metal ion is high, it may cause the death or abnormal behavior of fish, crustaceans, and plants raised. In addition, some substances are found to be toxic to specific breeding species such as catfish, ancient fish, willow moss, etc., regardless of concentration.
また、付着藻類の養分を除去する添加剤は、藻類自体を除去する方法ではない。そのため、養分の濃度が低い環境でも増えることができる藻類が繁茂すれば防藻効果が得られない。さらに、水槽用の既存の藻類抑制剤は、すでに発生している藻類に対する除去効果が乏しい。 Also, the additive that removes nutrients from adhering algae is not a method for removing the algae themselves. Therefore, if algae, which can proliferate even in an environment with low nutrient concentration, flourish, the anti-algae effect cannot be obtained. In addition, existing algae control agents for aquariums are poorly effective at removing already established algae.
特許文献１に記載の方法によれば、飼育生物に悪影響を及ぼすことなく防藻効果が得られる。しかし、グリーンヒドラを飼育することが必須条件であるために、グリーンヒドラ専用の飼育設備と飼育管理が必要である。しかも、グリーンヒドラは市中で広く販売されている生物ではないので、手軽に行える藻類除去手段であるとは言い難い。 According to the method described in Patent Document 1, an anti-algae effect can be obtained without adversely affecting reared organisms. However, since breeding green hydras is a prerequisite, breeding facilities and breeding management dedicated to green hydras are necessary. Moreover, since Green Hydra is not an organism that is widely sold in the market, it is difficult to say that it is an easy method for removing algae.
本発明は、上述した背景技術に鑑み、水槽で飼育する生物に安全で、藻類を除去する効果が高く、かつ手軽に扱える藻類抑制剤の提供を目的とする。 SUMMARY OF THE INVENTION In view of the background art described above, an object of the present invention is to provide an algae inhibitor that is safe for organisms reared in aquariums, highly effective in removing algae, and easy to handle.
即ち、本発明は下記［１］～［３］に記載の構成を有する。 That is, the present invention has the configurations described in [1] to [3] below.
［１］ヒドラリシンを含有することを特徴とする藻類抑制剤。 [1] An algae inhibitor characterized by containing hydralysin.
［２］前記ヒドラリシンが固体である前項１に記載の藻類抑制剤。 [2] The algae inhibitor according to the above item 1, wherein the hydralysin is solid.
［３］水棲生物飼育水槽において、水中のヒドラリシン濃度が０．１ｍｇ／Ｌ～１０ｍｇ／Ｌとなるように前項1または２に記載の藻類抑制剤を投入することを特徴とする藻類抑制方法。 [3] A method for suppressing algae, which comprises adding the algae inhibitor according to the above item 1 or 2 to a water tank for breeding aquatic organisms so that the hydralysine concentration in the water is 0.1 mg/L to 10 mg/L.
上記［１］に記載の藻類抑制剤によれば、ヒドラリシンが藻類を死滅させることによって防藻効果が得られる。また、ヒドラリシンはヒドラが有するタンパク質であるから、飼育生物に悪影響を及ぼすことなく防藻効果を得ることができる。また、水槽内に藻類抑制剤を投入するだけであるから、手軽に扱える。 According to the algae inhibitor described in [1] above, hydralysin kills algae, thereby providing an anti-algae effect. In addition, since hydralysin is a protein possessed by hydra, an anti-algae effect can be obtained without adversely affecting reared organisms. In addition, since the algae inhibitor is only put into the water tank, it can be handled easily.
上記［２］に記載の藻類抑制剤は、ヒドラリシンが固体であるから常温における安定性が優れており、長期保存が可能である。また、高温および低温に対する耐性が高いので物流管理が容易である。 In the algae inhibitor described in [2] above, since hydralysine is solid, it has excellent stability at room temperature and can be stored for a long period of time. In addition, since it has high resistance to high and low temperatures, distribution management is easy.
上記［３］に記載の藻類抑制方法によれば、ヒドラリシンによる防藻効果により、水槽内を手軽に美しく保つことができる。 According to the method for suppressing algae described in [3] above, the inside of the aquarium can be easily kept beautiful due to the anti-algae effect of hydralysine.
本発明の藻類抑制剤は有効成分としてヒドラリシンを含有している。 The algal inhibitor of the present invention contains hydralysin as an active ingredient.
ヒドラリシン（hydralysin）は、刺胞動物のヒドラ科に属し淡水に生息するヒドラが有するタンパク質であり、ヒドラリシン－１（hydralysin-1）、ヒドラリシン－２（hydralysin-2）、ヒドラリシン－３（hydralysin-3）を含む複数種のタンパク質の総称である。前記ヒドラリシンは藻類を死滅させる作用を有している。ヒドラのなかでも、体内で緑藻を共生させるグリーンヒドラはヒドラリシンを体外に分泌する。 Hydralysin is a protein possessed by hydra that belongs to the hydra family of cnidarians and lives in fresh water. ) is a generic term for multiple types of proteins including The hydralysin has the effect of killing algae. Among hydras, the green hydra, which coexists with green algae, secretes hydralysin to the outside of the body.
水槽をセッティングする際に水にヒドラリシンを入れておけば、微量の藻類が飼育生物等と共に持ち込まれ、あるいは大気中から持ち込まれたとしても、藻類を死滅させるので成長には至らない。また、既に藻類が成長して付着している水槽においても、ヒドラリシンを投入すれば死滅させて藻類を除去することができる。以下の説明において、ヒドラリシンによる藻類成長阻止効果および付着した藻類を除去する効果を、総括して「ヒドラリシンによる防藻効果」あるいは単に「防藻効果」と略す場合がある。 If hydralysin is added to the water when setting up the aquarium, even if a small amount of algae is brought in with the reared organisms or is brought in from the atmosphere, the algae will die and will not grow. In addition, even in a water tank where algae have already grown and adhered, adding hydralysin can kill the algae and remove the algae. In the following description, the effect of hydralysine to inhibit the growth of algae and the effect of removing adhering algae may be collectively abbreviated as "anti-algae effect by hydralysine" or simply "anti-algae effect".
ヒドラリシンによる防藻効果は、珪藻や緑藻等の各種藻類に対して有効である。これらの藻類は水槽内に多く出現する藻類であり、水中にヒドラリシンを含む藻類抑制剤を投入することによって藻類に対する防藻効果によって水槽内を美しく保つことができる。また、既に水槽の壁面、底砂、水中アクセサリーに藻類が付着している場合でも、藻類抑制剤を投入することによって水槽内を美しい状態に回復させることができる。また、ヒドラリシンは水棲生物であるヒドラに由来するタンパク質であるから、金属イオンのように水棲生物に悪影響を及ぼすことなく防藻効果を得ることができる。しかも、藻類抑制剤を水槽内に投入するだけであるから手軽に扱える。 The anti-algae effect of hydralysine is effective against various types of algae such as diatoms and green algae. These algae are algae that often appear in water tanks, and by adding an algae inhibitor containing hydralysine to water, the water tank can be kept beautiful due to its anti-algae effect. In addition, even if algae have already adhered to the walls, bottom sand, and underwater accessories of the tank, the inside of the tank can be restored to a beautiful state by adding the algae inhibitor. Moreover, since hydralysin is a protein derived from hydra, which is an aquatic organism, it can provide an anti-algae effect without adversely affecting aquatic organisms unlike metal ions. In addition, the algae inhibitor can be easily handled by simply putting it into the water tank.
水棲生物飼育水槽において、水中のヒドラリシン濃度は０．１ｍｇ／Ｌ～１０ｍｇ／Ｌの範囲であることが好ましい。ヒドラリシン濃度が１ｍｇ／Ｌ未満では防藻効果が少ない。一方、０．１ｍｇ／Ｌであれば十分な防藻効果が得られるので、１０ｍｇ／Ｌを超える高濃度に設定するメリットが乏しい。特に好ましいヒドラリシン濃度は１ｍｇ／Ｌ～４ｍｇ／Ｌである。また、ヒドラリシンの消耗によりヒドラリシン濃度が低下すると防藻効果も低下するので、藻類抑制剤を適宜追加投入して上記濃度を維持することが好ましい。 In an aquarium for breeding aquatic organisms, the hydralysine concentration in water is preferably in the range of 0.1 mg/L to 10 mg/L. If the hydralysine concentration is less than 1 mg/L, the anti-algae effect is small. On the other hand, if it is 0.1 mg/L, a sufficient anti-algae effect can be obtained, so there is little merit in setting a high concentration exceeding 10 mg/L. A particularly preferred hydralysine concentration is between 1 mg/L and 4 mg/L. In addition, when the concentration of hydralysine decreases due to consumption of hydralysin, the anti-algae effect also decreases, so it is preferable to add the algae-inhibiting agent appropriately to maintain the above-mentioned concentration.
ヒドラリシンはヒドラ由来のタンパク質であり、特にグリーンヒドラの分泌物の一部である場合は自然界において液状物として存在する。しかし、ヒドラリシンは、液体の状態でも、液体を粉末やペレット等の固体に加工してもどちらの状態でも、水中に分散させることによって上述した防藻効果が得られる。ただし、固体に加工したヒドラリシンは液体よりも常温における安定性が優れているので、長期保存が可能である。また、固体に加工することで、軽量化され嵩も低くなるだけでなく、高温および低温に対する耐性が高まるので、物流管理が容易である。さらに、固体であれば、餌等の他材料と混合して使用することができる。 Hydralysin is a hydra-derived protein that occurs in nature as a liquid, especially when it is part of the secretions of a green hydra. However, hydralysin can provide the above-mentioned anti-algae effect by dispersing it in water, whether it is in a liquid state or in a solid state such as powder or pellets. However, since hydralysin processed into a solid has superior stability at room temperature to liquid, it can be stored for a long period of time. In addition, processing into a solid not only reduces weight and volume, but also increases resistance to high and low temperatures, facilitating physical distribution management. Furthermore, if it is solid, it can be used by mixing with other materials such as feed.
本発明の藻類抑制剤は、ヒドラリシンの他に、水棲生物に無害なものである限り他の材料と混合して調製することができる。藻類抑制剤は水中のヒドラリシン濃度を所定値にすることで防藻効果が得られるので、藻類抑制剤中のヒドラリシン含有量に制限はない。藻類抑制剤にヒドラリシン以外の材料を混合する場合は、それらの材料の投入量とヒドラリシン投入量との割合に応じて適宜設定すればよい。 The algae inhibitor of the present invention can be prepared by mixing hydralysin with other materials as long as they are harmless to aquatic organisms. Since the algae-inhibiting agent can obtain an algae-preventing effect by adjusting the concentration of hydralysine in water to a predetermined value, there is no limit to the content of hydralysine in the algae-inhibiting agent. When a material other than hydralysine is mixed with the algae inhibitor, the amount may be appropriately set according to the ratio of the amount of these materials to be added and the amount of hydralysine to be added.
また、本発明の藻類抑制剤は、ヒドラ由来のヒドラリシン、例えばグリーンヒドラの分泌物を原料として作製する他、遺伝子組換え技術を用いて人工的に作製することができる。 In addition, the algae inhibitor of the present invention can be produced using hydralysin derived from hydra, such as the secretion of green hydra as a starting material, or can be artificially produced using genetic recombination technology.
例えば、大腸菌を用いた遺伝子組換え技術によってヒドラリシンを含む藻類抑制剤を安定して作製できる。大腸菌はヒドラリシンを産生しないが、大腸菌のゲノムにヒドラリシンの遺伝子を組み込むことでヒドラリシンを産生する遺伝子組換え大腸菌を作ることができる。遺伝子組換え大腸菌を大量に培養し、そこからヒドラリシンを取り出して精製することによって藻類抑制剤が得られる。そして、要すれば、水分を除去して粉体等の固体に加工する。アクアリウム用藻類抑制剤として用いる場合は、必ずしもヒドラリシンを純度１００％まで精製するには及ばず、培地や大腸菌の成分を含有していても支障はない。なお、上述した水中のヒドラリシン濃度（０．１ｍｇ／Ｌ～１０ｍｇ／Ｌ）はヒドラリシンの正味量である。 For example, a hydralysin-containing algal inhibitor can be stably produced by gene recombination technology using Escherichia coli. E. coli does not produce hydralysin, but genetically modified E. coli that produces hydralysin can be created by integrating the hydralysin gene into the genome of E. coli. An algae inhibitor is obtained by culturing a large amount of genetically modified E. coli and extracting and purifying hydralysin therefrom. Then, if necessary, the water is removed and processed into a solid such as powder. When used as an algae inhibitor for an aquarium, the hydralysin is not necessarily purified to a purity of 100%, and there is no problem even if it contains the components of the culture medium or Escherichia coli. The hydralysine concentration in water (0.1 mg/L to 10 mg/L) mentioned above is the net amount of hydralysine.
藻類抑制剤は、天然のヒドラリシンを原料とするものであっても、遺伝子組換え技術で得たヒドラリシンを原料とするものであっても、同等の防藻効果が得られる。しかし、藻類抑制剤を安定して効率良く製造するには、遺伝子組換えのヒドラリシンを用いる方が有利である。 The algae-inhibiting agent can obtain the same anti-algae effect regardless of whether it is made from natural hydralysin or from hydralysin obtained by genetic recombination technology. However, in order to stably and efficiently produce an algal inhibitor, it is more advantageous to use genetically modified hydralysin.
遺伝子組換えヒドラリシンを作製し、作製したヒドラリシンによるの防藻効果を検証した。
［ヒドラリシンの作製］
大腸菌のゲノムに、グリーンヒドラの分泌物から採取したヒドラリシンの遺伝子を組み込み、ヒドラリシンを産生する遺伝子組換え大腸菌を培養した。そして、大腸菌および培地を取り除いてヒドラリシンを取出し、さらに精製し乾燥させて純度１００％の粉末ヒドラリシンを得た。
［防藻試験］
１２ウェルのマルチウェルプレートを複数枚準備し、それぞれのプレートに珪藻（Achnanthidium minutissimum）または緑藻（Characium sp.）を入れてこれらを培養した。培養期間は、珪藻が２ヶ月、緑藻が３ヶ月であり、これらの期間の培養により、珪藻および緑藻がウェルの底面にしっかり付着した。各期間培養した後、ウェル内に浮遊している藻類を捨て、さらにミネラルウォーター（商品名「Volvic」）で洗浄した。上述した方法により、珪藻を付着させたマルチウエルプレートを３枚、緑藻を付着させたマルチウエルプレートを２枚を作製した。以下の説明において、珪藻を付着させたマルチウエルプレートを「珪藻プレート」と略称し、緑藻を付着させたマルチウエルプレートを「緑藻プレート」と略称する。
Gene-recombinant hydralysin was prepared and its anti-algae effect was verified.
[Preparation of hydralysin]
The gene for hydralysin extracted from the secretion of green hydra was integrated into the E. coli genome, and transgenic E. coli producing hydralysin was cultured. Then, E. coli and the medium were removed to obtain hydralysin, which was further purified and dried to obtain powdery hydralysin with a purity of 100%.
[Anti-algae test]
A plurality of 12-well multiwell plates were prepared, and diatoms (Achnanthidium minutissimum) or green algae (Characium sp.) were placed in each plate and cultured. The culture period was 2 months for diatoms and 3 months for green algae, and diatoms and green algae adhered firmly to the bottom of the wells by culturing during these periods. After culturing for each period, algae floating in the wells were discarded, and the wells were further washed with mineral water (trade name “Volvic”). Three multi-well plates to which diatoms were attached and two multi-well plates to which green algae were attached were prepared by the method described above. In the following description, the multi-well plate to which diatoms are adhered is abbreviated as "diatom plate", and the multi-well plate to which green algae is adhered is abbreviated as "green algae plate".
次に、前記ヒドラリシンとミネラルウォターを用いて、ヒドラリシン濃度が１ｍｇ／Ｌおよび４ｍｇ／Ｌの２種類のヒドラリシン液を調製した。そして、ヒドラリシン濃度１ｍｇ／Ｌのヒドラリシン液を１枚の珪藻プレートと１枚の緑藻プレートの各ウエルに入れ、ヒドラリシン濃度４ｍｇ／Ｌのヒドラリシン液を１枚の珪藻プレートに入れた。また比較例として、１枚の珪藻プレートおよび１枚の緑藻プレートにミネラルウォーター（ヒドラリシン濃度０ｍｇ／Ｌ）を入れた。各プレートを２０℃で静置し、継続して観察した。 Next, using the hydralysin and mineral water, two types of hydralysin solutions having hydralysin concentrations of 1 mg/L and 4 mg/L were prepared. Then, a hydralysine solution with a hydralysine concentration of 1 mg/L was put into each well of one diatom plate and one green alga plate, and a hydralysine solution with a hydralysine concentration of 4 mg/L was put into one diatom plate. As a comparative example, one diatom plate and one green alga plate were filled with mineral water (hydralysine concentration: 0 mg/L). Each plate was placed at 20°C and observed continuously.
観察期間中、各プレートの底面を１日１回透過型スキャナーで撮影し、撮影した画像を画像解析ソフト（ImageJ）によりデータの二値化を行い、藻類が付着している面積を割り出した。観察初日の藻類の付着面積を１００％とし、初日に対する観察日の付着面積の割合で防藻効果を評価した。結果を表１に示す。 During the observation period, the bottom surface of each plate was photographed with a transmission scanner once a day, and the photographed images were binarized using image analysis software (ImageJ) to determine the area where algae adhered. Taking the algae adhesion area on the first day of observation as 100%, the anti-algae effect was evaluated by the ratio of the adhesion area on the observation day to the first day. Table 1 shows the results.
表１より、ヒドラリシン液により珪藻は２７日後に２０％にまで減少している。換言すると、８０％の珪藻を除去することができた。同様に、緑藻は２０日後に３０％まで減少した。一方、ヒドラリシンを含まない比較例は藻類が減少しなかった。これらの結果より、ヒドラリシンが防藻効果を有していることは明白である。 From Table 1, the hydralysin solution reduced diatoms to 20% after 27 days. In other words, 80% of diatoms could be removed. Similarly, green algae decreased by 30% after 20 days. On the other hand, algae did not decrease in the comparative example containing no hydralysin. From these results, it is clear that hydralysine has an anti-algae effect.
本発明の藻類抑制剤は水槽内の藻類除去に利用できる。
The algae control agent of the present invention can be used to remove algae in aquariums.
Claims (3)
3. A method for suppressing algae, which comprises adding the algae inhibitor according to claim 1 or 2 to a water tank for breeding aquatic organisms so that the hydralysine concentration in the water is 0.1 mg/L to 10 mg/L.
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| JP2019154395A (en) * | 2018-03-16 | 2019-09-19 | 国立大学法人神戸大学 | Rearing device for aquatic animal |
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| JP2019154395A (en) * | 2018-03-16 | 2019-09-19 | 国立大学法人神戸大学 | Rearing device for aquatic animal |
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