JP2022100464A - Agent for increasing collagen - Google Patents

Abstract

To provide a new material that exhibits the effect of increasing collagen even when applied to organisms.SOLUTION: Oren-gedoku-to extract can increase the amount of collagen even when applied to organisms, so that it can be used as an active ingredient of an agent for increasing collagen.SELECTED DRAWING: None

Description

The present invention relates to a collagen increasing agent.

Collagen is a type of extracellular matrix and is not only essential for the maintenance of tissue and organ morphology, but also plays an important role in physiological conditions such as wound healing and tissue repair processes. Among them, collagen in the skin is reduced by aging and light stimulation, which is one of the causes of wrinkles and sagging, which is a decrease in skin aging.

Typical examples of ingredients to be blended to promote the production of collagen having a function of giving elasticity to the skin are retinoic acid and retinol. Tretinoin acid is used as an active ingredient in wrinkle-relieving medicines, but it is highly irritating and requires a doctor's prescription. The strength of the physiological activity of retinol is about 1/100 of that of retinoic acid, and since it is highly safe, it is used in cosmetics.

In recent years, research has been conducted on other materials that have the effect of increasing collagen. For example, Patent Document 1 discloses a collagen production-promoting composition containing a cell of Pediococcus acidilactici or a processed product thereof, and Patent Document 2 discloses Lilium rubellum (Lilium rubellum). Lilium rubellum) A collagen production promoter characterized by containing a water extract as an active ingredient has been disclosed.

Japanese Unexamined Patent Publication No. 2015-096475 Japanese Unexamined Patent Publication No. 2016-069332

As mentioned above, retinoic acid has a problem of irritation, and retinol has a problem that the effect is not so high. Further, the materials described in Patent Documents 1 and 2 have only been confirmed to have an effect at the cellular level, and do not show how much the effect is exhibited when actually applied to a living body.

Therefore, an object of the present invention is to provide a new material which has an effect of increasing collagen even when applied to a living body.

As a result of diligent studies, the present inventor has found that orengedokuto extract exhibits an increasing effect on collagen even when applied to a living body. The present invention has been completed by further studies based on such findings.

That is, the present invention provides the inventions of the following aspects.
Item 1. Collagen increasing agent containing orengedokuto extract.
Item 2. Item 2. The collagen increasing agent according to Item 1, which is used to increase the amount of collagen in the dermis layer of the skin.
Item 3. Item 2. The collagen increasing agent according to Item 1 or 2, which is used to increase the amount of collagen in the outer corner of the eye.

According to the present invention, there is provided a new material which has an effect of increasing collagen even when applied to a living body.

The ultrasonic dermis image before and after taking the collagen increasing agent of this invention is shown. The graph of the dermis collagen amount before and after taking the collagen increasing agent of this invention is shown.

The collagen-increasing agent of the present invention is characterized by containing orengedokuto extract. Hereinafter, the collagen-increasing agent of the present invention will be described in detail.

Orengedokuto extract The collagen-increasing agent of the present invention contains orengedokuto extract as an active ingredient. Orengedokuto is a mixed crude drug consisting of Scutellaria baicalensis, Coptis chinensis, Scutellaria baicalensis, and Scutellaria baicalensis, which is based on "Scutellaria baicalensis".

In the present invention, the mixing ratio of the crude drugs constituting Orengedokuto is not particularly limited, but is usually 1.5 to 4 parts by weight, preferably 2 to 3 parts by weight of Ougon; 0.5 to 3 parts by weight of Ouren. It is preferably 0.75 to 2 parts by weight; 0.5 to 4 parts by weight of Sanshishi, preferably 1 to 3 parts by weight; 0.5 to 4 parts by weight of Oubaku, preferably 0.75 to 3 parts by weight.

Suitable examples of the crude drug formulation used for producing the orengedokuto extract used in the present invention are 3 parts by weight of Scutellaria baicalensis, 2 parts by weight of Coptis chinensis, 1.5 parts by weight of Coptis chinensis, and 1.5 parts by weight of Scutellaria baicalensis. Can be mentioned.

The form of the extract of Orengedokuto may be any of a liquid extract such as a flowing extract and a soft extract, or a solid dry extract powder.

The liquid extract of Orengedokuto can be obtained by extracting and treating a mixed crude drug according to Orengedokuto and concentrating the obtained extract as necessary. The extraction solvent used in the extraction treatment is not particularly limited, and examples thereof include water or hydrous ethanol, preferably water. Further, the dried extract powder of Orengedokuto can be obtained by drying a liquid extract. The method of the drying treatment is not particularly limited, and for example, a spray-drying method, a method of adding an appropriate adsorbent (for example, anhydrous silicic acid, starch, etc.) to a soft extract having an increased concentration of the extract to obtain an adsorbed powder, and the like are used. Can be mentioned.

In the present invention, as the orengedokuto extract, the extract prepared by the above-mentioned method may be used, or a commercially available extract may be used. For example, as the dried extract powder of orengedokuto, orengedokuto dried extract-AT (manufactured by Nippon Powder Chemicals Co., Ltd.), orengedokuto dried extract-F (manufactured by Alps Pharmaceutical Co., Ltd.), etc. are known as products. It is available and can be obtained commercially.

In the collagen-increasing agent of the present invention, the content of the orengedokuto extract is not particularly limited as long as the effect of the present invention is exhibited, but it is usually 10 to 100% by weight in terms of the dry extract powder amount of the orengedokuto extract. %, Preferably 20 to 90% by weight, more preferably 40 to 80% by weight, still more preferably 60 to 70% by weight. In the present invention, the dry extract powder conversion of orengedokuto is the amount of the dried extract powder of orengedokuto itself, and the liquid extract of orengedokuto is used. If so, it is the amount converted into the remaining amount after removing the solvent. When the dried extract powder of Orengedokuto contains an additive such as an adsorbent added at the time of production, the amount is the amount excluding the additive.

Other Ingredients The collagen-increasing agent of the present invention may consist of orengedokuto extract alone, or may contain additives or bases according to the pharmaceutical form. Such additives and bases are not particularly limited as long as they are pharmaceutically acceptable, but for example, excipients, binders, disintegrants, lubricants, tonicity agents, plasticizers, etc. Dispersants, emulsifiers, solubilizers, wetting agents, stabilizers, suspending agents, pressure-sensitive agents, coating agents, brighteners, water, fats and oils, waxes, hydrocarbons, fatty acids, higher alcohols , Esters, water-soluble polymers, surfactants, metal soaps, lower alcohols, polyhydric alcohols, pH adjusters, buffers, antioxidants, UV inhibitors, preservatives, emulsifiers, fragrances, powders, Examples include thickeners, pigments, chelating agents and the like. These additives may be used alone or in combination of two or more. The contents of these additives and bases are appropriately set according to the types of additives and bases to be used, the form of the collagen-increasing agent, and the like.

In addition to the orengedokuto extract, the collagen-increasing agent of the present invention may contain other nutritional components and pharmacological components, if necessary. Such nutritional components and pharmacological components are not particularly limited as long as they are pharmaceutically acceptable, but for example, antihypertensive agents, stomachic agents, digestive agents, intestinal regulators, antispasmodics, mucosal repair agents, antihistamines, and astringents. Agents, antiemetics, antitussives, sputum, anti-inflammatory enzyme agents, sedative hypnotics, antihistamines, caffeines, cardiotonic diuretics, antibacterial agents, vasoconstrictors, vasoconstrictors, local anesthetics, crude drug extracts, vitamins, menthol Kind and the like. These nutritional components and pharmacological components may be used alone or in combination of two or more. Further, the content of these components is appropriately set according to the type of the component to be used and the like.

Pharmaceutical form The pharmaceutical form of the collagen-increasing agent of the present invention is not particularly limited as long as it can be orally administered, and is, for example, a powder, a fine granule, a granule, a tablet, a troche, a chewable agent, or a capsule. (Soft capsules, hard capsules), solid formulations such as rounds; semi-solid formulations such as jelly; liquid formulations such as liquids, suspensions and syrups, preferably granules or tablets. Can be mentioned.

Production method The method for producing the collagen-increasing agent of the present invention may be formulated by using the above-mentioned crude drug components according to a usual formulation method adopted in the pharmaceutical field.

Use The collagen-increasing agent of the present invention is used for the purpose of increasing the amount of collagen.

The tissue or organ in which the amount of collagen should be increased is not particularly limited, and examples thereof include a skin dermis layer, gums, and cartilage, and a skin dermis layer is preferable. Further, when the collagen increasing agent of the present invention is used to increase the amount of collagen in the skin dermis layer, the location of the skin dermis layer on the body surface is not particularly limited, and the face, neck, trunk, upper limbs, and lower limbs are not particularly limited. However, the face is preferably mentioned, and the outer corners of the eyes are more preferably mentioned.

Dosage and Usage The collagen-increasing agent of the present invention is used by oral administration. The dose of the collagen-increasing agent of the present invention is appropriately set according to the age, constitution, degree of symptoms, etc. of the administration subject, and for example, the amount of orengedokuto extract per human per day ( The dry extract equivalent amount) may be 500 to 2000 mg, preferably 800 to 1800 mg, and may be taken 1 to 4 times a day, preferably 2 to 3 times a day.

The timing of administration during the day is not particularly limited and may be pre-meal, post-meal, or inter-meal, but pre-meal or inter-meal is preferable. Further, from the viewpoint of further enhancing the collagen increasing effect, the collagen increasing agent of the present invention is preferably taken continuously for, for example, 3 days or more, preferably 5 days or more, and more preferably 10 days or more.

Hereinafter, the present invention will be specifically described with reference to Examples, but the present invention is not limited to these Examples.

[Test Example 1]
(1) Preparation of collagen-increasing agent As raw material crude drugs, 3 parts by weight of Ogon, 2 parts by weight of Sanshishi, 1.5 parts by weight of Ouren, and 1.5 parts by weight of Oubaku were used, and after chopping these, 10 times the weight of water was used. The mixture was extracted at about 100 ° C. for 1 hour and centrifuged to obtain an extract. The extract was concentrated under reduced pressure and dried using a spray dryer to obtain orengedokuto extract powder. The drying with a spray dryer was carried out by dropping the extract onto an atomizer having a rotation speed of 10000 rpm and supplying hot air with air at 150 ° C. Then, granulation was performed and tablets were formed to obtain a collagen increasing agent. The daily dose of the collagen-increasing agent was 1600 mg in terms of the amount of orengedokuto extract (dry extract equivalent amount).

(2) Experimental method Nine men and women in their twenties were allowed to take the above collagen-increasing agent for 10 days (twice a day). The collagen score of the outer corner of the left eye before and after administration was measured using an ultrasonic dermis imaging device (device name: DermaLab (registered trademark), model number: DermaLab, manufactured by Cortex Technology, measurement item: collagen score). The measurement was performed 3 times each, and the average value was used as the measured value (the two collagen score measurements were set to be within ± 5. The room temperature was 20 ° C., the humidity was 65%, and the measurement was acclimatized for 15 minutes. I went later.

An example of the obtained ultrasonic dermis image (before and after taking) is shown in FIG. In each image shown in FIG. 1, the left side shows the stratum corneum side and the left side shows the dermis layer side. In this ultrasonic dermis image, high density areas of dermal collagen fibers are displayed in yellow and low density areas are displayed in green. As shown in FIG. 1, it was confirmed that the amount of collagen increased remarkably after taking the drug.

In addition, in FIG. 2, the mean value ± standard error of the measurement result of the collagen score was calculated, and the test was performed by the corresponding t-test using Microsoft (registered trademark) Excel (statistical analysis software: Excel statistics) (significance level). Is 5% in the two-sided test). As shown in FIG. 2, a significant (*: p <0.05) increase in collagen score was confirmed. From this result, it was confirmed that the amount of collagen increased after taking the drug.

(3) Comparison with collagen increasing ability by a mixture of Coptis chinensis extract and Coptis chinensis extract The collagen increasing agent of the present invention has an excellent collagen increasing effect, and Orengedokuto extract acts as an active ingredient. This is the case when a mixture of Coptis chinensis and Coptis chinensis extracts (a mixture of Coptis chinensis extract and Coptis chinensis extract) and Orengedokuto extract, which are the constituent crude drugs of Orengedokuto, are subjected to the following cell tests, respectively. , Orengedokuto extract can be confirmed to have a better collagen-increasing effect.

(Cell test method)
Normal human skin fibroblasts (NHDF) are cultured in 24-well culture plates. More specifically, the plates are seeded at a density of 1.0 × 10 ⁴ cells / well and cultured at 37 ° C. for 72 hours in an environment of 5% by volume carbon dioxide and 95% by volume air. For the culture medium, a medium containing Dulvecco's Modified Eagle Medium (DMEM) at a concentration of 2% by mass of bovine fetal serum (FBS) was used, and the concentration of Orengedokuto extract and the concentration of Oulen extract and Oubaku extract were used. The concentration (that is, the total amount of the orengedokuto and the orengedokuto extract) of the mixture (mixed weight ratio of the orengedokuto extract and the orengedokuto extract = 1: 1) is added so as to be 0.01% by mass, respectively. After completion of the culture, the amount of collagen in the culture supernatant is measured by ELISA.

Claims (3)

Collagen increasing agent containing orengedokuto extract. The collagen-increasing agent according to claim 1, which is used to increase the amount of collagen in the dermis layer of the skin. The collagen-increasing agent according to claim 1 or 2, which is used to increase the amount of collagen in the outer corner of the eye.

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