JP2022088541A - デジタルホログラフィ顕微鏡検査および無傷の(untouched)末梢血白血球を用いる高精度の5部鑑別(5-part Differential) - Google Patents
デジタルホログラフィ顕微鏡検査および無傷の(untouched)末梢血白血球を用いる高精度の5部鑑別(5-part Differential) Download PDFInfo
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Abstract
Description
6μm未満の被写界深度を有するデジタルホログラフィ顕微鏡デバイスと;
マイクロ流体デバイスと;
少なくとも1つの細胞の細胞タイプを判定するように構成された検出システムとを含むデバイスに関する。
関連し、図面は本発明の概念および原理を説明する役割を果たす。他の実施形態および記載される利点の多くを、図面との関連で引き出すことができる。図面の要素は必ずしも互いに縮尺通りではない。同一の、機能的に等価な、等しく作用する構成、および構成要素は、特記しない限り、図面において同一の参照数字で示される。
なる情報を判定することができる。
きる。
volumetric image data」、MICCAI、2015年、710~718頁;およびZheng他、「Four-chamber heart modeling and automatic segmentation for 3-D cardiac CT volumes using marginal space learning and steerable features」、IEEE Transactions on Medical Imaging、第27巻、第11号、
1668~1681頁、2008年により開発された、マージナルスペースディープラーニング(MSDL)アーキテクチャなどの最新の検出方法を使用して、白血球の5タイプ分類を行うことができる。機械学習分類方法の大部分が対の分類器を構築するが、MSDLは本来、マルチラベル分類器である。したがって、マルチラベル分類の問題について、畳込みネットワークを直接トレーニングすることができる。例えば、500の細胞、例えば各カテゴリーにつき100の細胞をトレーニングに使用することができ、残りの細胞を検査に使用することができる。
。
6μm未満の被写界深度を有するデジタルホログラフィ顕微鏡デバイスと;
マイクロ流体デバイスと;
少なくとも1つの細胞の細胞タイプを判定するように構成された検出システムとを含むデバイスに関する。
複雑な場合もある。
Claims (15)
- 媒体中の少なくとも1つの細胞の細胞タイプのマーカフリー検出のための方法であって、
少なくとも1つの細胞を含む媒体をマイクロ流体デバイスに流す工程と、
デジタルホログラフィ顕微鏡デバイスにより、マイクロ流体デバイス内の少なくとも1つの細胞の画像を取得する工程であって、画像は6μm未満の被写界深度で取得される工程と、
少なくとも1つの細胞の細胞タイプを判定する工程と
を含む前記方法。 - 画像は、0.6μm未満の方位分解能で取得される、請求項1に記載の方法。
- 検出は、少なくとも1つの細胞を含む媒体をマイクロ流体デバイスに流しながら実行される、請求項1または2に記載の方法。
- マイクロ流体デバイス内のフローは、少なくとも1つの細胞の画像が取得される領域で、少なくとも層流である、請求項3に記載の方法。
- マイクロ流体デバイスは、少なくとも1つの細胞の画像が取得される領域にマイクロチャネルを含み、該マイクロチャネル内で少なくとも1つのシースフローによって層流が生じる、請求項4に記載の方法。
- デジタルホログラフィ顕微鏡デバイスの参照および/または検出ビームの波面に垂直なマイクロチャネルの表面は、基本的に平面である、請求項5に記載の方法。
- デジタルホログラフィ顕微鏡デバイスの視野は、少なくとも1つの細胞の画像が取得される領域におけるマイクロ流体デバイスの幅の1.0倍以下である、請求項1~6のいずれか1項に記載の方法。
- 少なくとも1つの細胞は血球である、請求項1~7のいずれか1項に記載の方法。
- 血球は白血球である、請求項8に記載の方法。
- 少なくとも1つの細胞の細胞タイプを判定する工程は、ディープラーニングネットワークを使用して実行される、請求項1~9のいずれか1項に記載の方法。
- デジタルホログラフィ顕微鏡デバイスの参照ビームおよび検出ビームは軸外である、請求項1~10のいずれか1項に記載の方法。
- 媒体中の少なくとも1つの細胞の細胞タイプのマーカフリー検出のためのデバイスであって、
6μm未満の被写界深度を有するデジタルホログラフィ顕微鏡デバイスと;
マイクロ流体デバイスと;
少なくとも1つの細胞の細胞タイプを判定するように構成された検出システムと
を含む前記デバイス。 - デジタルホログラフィ顕微鏡デバイスは、0.6μm未満の方位分解能を有する、請求項12に記載のデバイス。
- マイクロ流体デバイスは、少なくとも1つの細胞の画像が取得される領域にマイクロチャネルを含み、マイクロ流体デバイスは、マイクロチャネル内に層流を生じさせる手段をさらに含む、請求項12または13に記載のデバイス。
- 検出システムはディープラーニングネットワークを含む、請求項12~14のいずれか1項に記載のデバイス。
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US11373278B2 (en) * | 2016-09-30 | 2022-06-28 | University Of Utah Research Foundation | Lensless imaging device |
EP3669227B8 (en) | 2017-08-15 | 2024-02-28 | Siemens Healthineers AG | Identifying the quality of the cell images acquired with digital holographic microscopy using convolutional neural networks |
ES2837438T3 (es) * | 2017-09-29 | 2021-06-30 | Siemens Healthcare Gmbh | Detección específica de malaria con microscopía óptica digital |
JP2019135939A (ja) * | 2018-02-07 | 2019-08-22 | 澁谷工業株式会社 | 細胞カウント方法および細胞カウント装置ならびに細胞培養方法 |
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