JP2022047468A - Skin external preparation - Google Patents

Abstract

To provide an active ingredient for a skin external preparation which is derived from a natural product, has excellent biological safety, prevents and mitigates skin inflammation or aging caused by internal factors such as aging or external factors such as UV light and environmental pollutants, keeps skin young and healthy, and makes skin smooth.SOLUTION: The skin external preparation comprises green rooibos extract as an active ingredient.SELECTED DRAWING: None

Description

The present invention relates to an external skin preparation containing a plant-derived ingredient as an active ingredient.

In recent years, from the viewpoint of biosafety, various plant-derived components have been developed as compounding components for external skin preparations. However, plant-derived components have problems in terms of efficacy and stability. In particular, in the case of plant-derived components, there is a problem that they may not exhibit sufficient effectiveness.

In view of the problems of the prior art, the present inventors have newly found that the extract of green rooibos is useful as an ingredient of excellent cosmetics.

Conventionally, for example, a skin external preparation containing a rooibos extract as an active ingredient is known from Patent Documents 1 to 3, but a skin external preparation containing a green rooibos extract as an active ingredient is not known. rice field. Further, a skin tissue protectant containing asparagus contained in green rooibos as an active ingredient is also known from Patent Document 4, but asparagus is extremely easily decomposed and has a problem in stability as an active ingredient of an external skin preparation. rice field.

Japanese Patent Laid-Open No. 05-271063 Japanese Patent Application Laid-Open No. 05-271089 Japanese Patent Laid-Open No. 06-128121 JP 2009-263243

The present invention is a skin external preparation containing an extract of green rooibos.

The present invention can provide a skin external preparation having excellent biosafety and effectiveness by using an extract of green rooibos as an active ingredient.

Hereinafter, preferred embodiments of the present invention will be described in detail.
First, the extract material rooibos (Aspalathus linearis) is a plant belonging to the genus Aspalathus in the family Leguminosae. In the present invention, non-fermented, green rooibos (green rooibos) is used. As the site of use, whole plants or leaves are preferable.

The green rooibos used as the extraction material may be raw, or may be pre-dried or semi-dried. Further, as the shape, the collected material can be used as it is, or any of the dried and pulverized products can be used.

The extract is prepared by washing the extracted material as necessary to remove foreign substances, then cutting or crushing the extract as it is or drying it as necessary, and contacting it with an extraction solvent for extraction. .. Extraction can be performed by contacting with an extraction solvent according to a conventional method such as a dipping method, but a supercritical extraction method can also be used in addition to the dipping method.

Extraction solvents include water; lower alcohols such as methanol, ethanol and propanol; polyhydric alcohols such as ethylene glycol, propylene glycol, 1,3-butylene glycol and glycerin; ethyl acetate, butyl acetate, methyl propionate and the like. Esters; Ketones such as acetone and methyl ethyl ketone; Ethers such as ethyl ether and isopropyl ether; Hydrocarbon-based solvents such as n-hexane, toluene and chloroform, etc., which may be used alone or in admixture of two or more. Used.

Among these extraction solvents, water, lower alcohols, polyhydric alcohols, etc. are used in the present invention from the viewpoint of skin irritation and effectiveness, and from the viewpoint that they can be widely applied to cosmetics. A hydrophilic solvent is suitable. Preferred examples of using this hydrophilic solvent include, for example, water, lower alcohols (particularly ethanol), or polyhydric alcohols (particularly 1,3-butylene glycol) alone, or water and lower alcohols. The use of a mixed solvent with (particularly ethanol) or a mixed solvent with water and polyhydric alcohols (particularly 1,3-butylene glycol, glycerin) can be mentioned. Among them, water alone or water and 1,3 -A mixed solvent of butylene glycol is particularly preferable.

When a mixed solvent is used, for example, in the case of a mixed solvent of water and 1,3-butylene glycol, the volume ratio (hereinafter the same) is 1:10 to 20: 1, and the mixed solvent of water and ethanol is used. If there is, it is preferably in the range of 1:10 to 25: 1, and in the case of a mixed solvent of water and glycerin, it is preferably in the range of 1:10 to 20: 1.

When preparing the extract, the pH is not particularly limited, but is generally preferably in the range of 3 to 9. In this sense, if necessary, an alkali adjusting agent such as sodium hydroxide, sodium carbonate, or potassium hydroxide, or an acid adjusting agent such as citric acid, hydrochloric acid, phosphoric acid, or sulfuric acid may be added to the extraction solvent, if desired. The pH may be adjusted to the above.

Extraction conditions such as extraction temperature and extraction time differ depending on the type and pH of the solvent used, but for example, when water or 1,3-butylene glycol or a mixed solution of water and 1,3-butylene glycol is used as the solvent. If so, the extraction temperature is preferably in the range of 0 ° C to 90 ° C. The extraction time is preferably in the range of 1 to 168 hours (1 hour to 1 week).

The extract prepared as described above may generally have a pH adjusted to 3 to 8 and then used as it is as a skin external preparation, or may be used as a desired concentration by concentration under reduced pressure or the like. May be. Further, the extract may be a dried product by a conventional method such as a spray drying method.

Examples of skin external preparations (external pharmaceutical parts, external pharmaceutical products and cosmetics) containing the extract of the present invention include emulsions, creams, lotions, essences, gels, packs, sheet masks, lipsticks, foundations, liquid foundations, etc. Makeup press powder, cheeks, white powder, facial cleansers, body shampoos, slimming agents, hair shampoos, soaps, etc., and hair growth agents, hair nourishing agents, bathing agents, etc. are also included, but are limited to these. It is not something that will be done.

The amount of the active ingredient according to the present invention in a skin external preparation (external pharmaceutical parts, quasi-drugs and cosmetics) is generally 0.0002 to 1.0% by weight in the case of basic cosmetics as its solid content. (Solid content% by weight, the same applies hereinafter), preferably in the range of 0.002 to 0.2% by weight, and in the case of make-up cosmetics, generally 0.0002 to 1.0% by weight, preferably 0.002 to 0. It is in the range of 2% by weight, and in the case of cosmetics for cleaning, it is generally in the range of 0.002 to 10.0% by weight. In the case of hair cosmetics, the solid content of the extract is generally 0.00001 to 5.0% by weight, preferably 0.0001 to 3.0% by weight.

Here, examples of the oily component include olive oil, jojoba oil, castor oil, soybean oil, rice oil, rice germ oil, palm oil, palm oil, cocoa oil, meadowfoam oil, sheer butter, tea tree oil, and avocado oil. , Macadamia nut oil, bergamot oil, lavender oil, rose oil, bergamot oil, chamomile oil and other plant-derived oils and fats such as squalane; vitamin A oil; mink oil, turtle oil and other animal-derived oils and fats; Rows such as carnauba wax, rice wax and lanolin; hydrocarbons such as liquid paraffin, vaseline, paraffin wax and squalane; fatty acids such as myristic acid, palmitic acid, stearic acid, oleic acid, isostearic acid and cis-11-eicosenoic acid. Classes; higher alcohols such as lauryl alcohol, cetanol, pantothenyl alcohol, stearyl alcohol; synthesis of isopropyl myristate, isopropyl palmitate, butyl oleate, 2-ethylhexyl glyceride, higher fatty acid octyldodecyl (octyldodecyl stearate, etc.) Examples thereof include esters and synthetic triglycerides.

Examples of the surfactant include polyoxyethylene alkyl ether, polyoxyethylene fatty acid ester, polyoxyethylene sorbitan fatty acid ester, glycerin fatty acid ester, polyglycerin fatty acid ester, polyoxyethylene glycerin fatty acid ester, and polyoxyethylene hydrogenated castor oil. Nonionic surfactants such as polyoxyethylene sorbitol fatty acid esters; fatty acid salts, alkyl sulfates, alkylbenzene sulfonates, polyoxyethylene alkyl ether sulfates, polyoxyethylene fatty amine sulfates, polyoxyethylene alkylphenyl ether sulfates , Polyoxyethylene alkyl ether phosphates, α-sulfonated fatty acid alkyl ester salts, polyoxyethylene alkyl phenyl ether phosphates and other anionic surfactants; quaternary ammonium salts, primary to tertiary fatty amine salts, Cationic surfactants such as trialkylbenzylammonium salt, alkylpyridinium salt, 2-alkyl-1-alkyl-1-hydroxyethylimidazolinium salt, N, N-dialkylmorphonium salt, polyethylene polyamine fatty acid amide salt; N , N-dimethyl-N-alkyl-N-carboxymethylammoniobetaine, N, N, N-trialkyl-N-alkylene ammoniocarboxybetaine, N-acylamide propyl-N', N'-dimethyl-N' An amphoteric surfactant such as -β-hydroxypropylammoniosulfobetaine can be used.

Emulsifiers and / or emulsifying aids are derived from stevia derivatives such as enzyme-treated stevia, saponins or derivatives thereof, casein or salts thereof (sodium, etc.), sugar-protein complexes, sucrose or esters thereof, lactose, soybeans. Water-soluble polysaccharides, soybean-derived protein and polysaccharide complexes, lanolin or derivatives thereof, cholesterol, stevia derivatives (stevia enzyme-treated products, etc.), silicates (aluminum, magnesium, etc.), carbonates (calcium, sodium, etc.) saponins and The derivative, lecithin and its derivative (hydrogenated lecithin, etc.), lactic acid bacteria fermented rice, lactic acid bacteria fermented sprouted rice, lactic acid bacteria fermented grains (wheat, beans, miscellaneous grains, etc.) and the like can also be blended.

As the moisturizing agent, examples of the moisturizing agent include glycerin, propylene glycol, dipropylene glycol, 1,3-butylene glycol, polyethylene glycol, sorbitol, xylitol, sodium pyrrolidone carboxylate and the like, and saccharides such as trehalose and raffinose. , Mucopolysaccharides (eg, hyaluronic acid and its derivatives, hyaluronic acid fermented liquid, chondroitin and its derivatives, heparin and its derivatives, etc.), elastin and its derivatives, collagen and its derivatives, collagen peptides, NMF-related substances, lactic acid, urea. , Higher fatty acid octyldodecyl, seaweed extract, estradiol, various amino acids and derivatives thereof.

Examples of the thickener include brown algae such as alginic acid, agar, carrageenan and fucoidan, components derived from green algae or red algae; polysaccharides such as pectin and aloe polysaccharides; gums such as tragant gum, locust bean gum, xanthan gum and guar gum; Cellulose derivatives such as methyl cellulose, hydroxyethyl cellulose and hydroxypropyl cellulose; synthetic polymers such as carrageenan vinyl polymer, alkyl modified carboxyvinyl polymer, polyvinyl alcohol, polyvinylpyrrolidone, acrylic acid / methacrylic acid copolymer; hyaluronic acid and its derivatives ; Polyglutamic acid and its derivatives, polyacrylic acid and the like can be mentioned.

Anti-inflammatory agents include allantoin, dipotassium glycyrrhizinate, monoammonium glycyrrhizinate, β-glycyrrhetinic acid, stearyl glycyrrhetinate, ε-aminocaproic acid, d-camphor, dl-camphor, zinc oxide, pantenol, pyridoxine hydrochloride, and riboflavin. Or there are derivatives thereof and the like.

Examples of antiseptic / bactericidal agents include urea; paraoxybenzoic acid esters such as urea; benzoic acid or a salt thereof, methyl paraoxybenzoate, ethyl paraoxybenzoate, propyl paraoxybenzoate, butyl paraoxybenzoate; phenoxyethanol, dichlorophene, hexachlorophene. , Chlorhexidine hydrochloride, benzalkonium chloride, salicylic acid, sodium salicylate, pyrithione zinc, benzalkonium chloride, ethanol, undecylenic acid, phenols, alkylisoquinolinium bromide, resorcin, jamar (imidazole dainylurea), isopropylmethyl Phenol, triclosan, trichlorocarbanide, trichlorohydroxydiphenol ether, hinokithiol, 1,2-pentanediol, propanediol, concentrated benzalkonium chloride solution 50, essential oils such as peppermint oil and eucalyptus oil, bark dry distillate, radish There are fermented liquids, plant-derived ethanol such as sugar cane and corn, or 1,3-butylene glycol and the like.

Examples of the cell activator include pantothenyl alcohol, menthol, dl-menthol, γ-oryzanol and the like.

Examples of the anti-acne agent include sulfur, salicylic acid or a salt thereof, Photosensitizer No. 201, pyridoxine dicaprylate and the like.

The powder components include, for example, sericite, titanium oxide, talc, kaolin, bentonite, zinc oxide, magnesium carbonate, magnesium oxide, zirconium oxide, barium sulfate, silicic acid anhydride, mica, nylon powder, polyethylene powder, silk powder, etc. There are cellulose-based powders, grain (rice, wheat, corn, millet, etc.) powders, beans (soybeans, azuki, etc.) powders, and the like.

Examples of the ultraviolet absorber include ethyl paraaminobenzoate, ethylhexyl paradimethylaminobenzoate, amyl salicylate and its derivatives, 2-ethylhexyl paramethoxycinnamate, octyl silicate, oxybenzone, 2,4-dihydroxybenzophenone, 2-hydroxy-4. -Methoxybenzophenone-5-sulfonate, 4-tersial butyl-4-methoxybenzoylmethane, 2- (2-hydroxy-5-methylphenyl) benzotriazole, urocanic acid, ethyl urocanate, aloe extract, etc. ..

Examples of the antioxidant include carotenoids such as butylhydroxyanisole, butylhydroxytoluene, propyl gallate, and astaxanthin, vitamin E and its derivatives (for example, tocopherol acetate, tocopherol nicotinic acid ester), vitamin A or its derivatives (palmitic acid). Retinol etc.) etc.

In addition, as whitening agents, ellagic acid and its derivatives, resorcinol derivatives, 4-methoxysalicylic acid potassium salt, magnolignan (5,5'-dipropyl-biphenyl-2,2'-diol), hydroxybenzoic acid and its derivatives, vitamins One or more selected from E and its derivatives, α-hydroxy acids, nicotinic acid derivatives, and AMP (adenosine monophosphate, adenosine monophosphate) can be mentioned.

Examples of the resorcinol derivative include 4-n-butylresorcinol and 4-isoamylresorcinol, and examples of the 2,5-dihydroxybenzoic acid derivative include 2,5-diacetoxybenzoic acid and 2-acetoxy-5-hydroxybenzoic acid. , 2-Hydroxy-5-propionyloxybenzoic acid and the like, and α-hydroxy acids include, for example, lactic acid, malic acid, succinic acid, citric acid, α-hydroxyoctanoic acid and the like. Kodiic acid and its derivatives, ascorbic acid and its derivatives, hydroquinone or its derivatives, ellagic acid and its derivatives, nicotinic acid and its derivatives, resorcinol derivatives, tranexamic acid and its derivatives, 4-methoxysalicylic acid potassium salt, magnolignan (5, 5'-dipropyl-biphenyl-2, 2'-diol), hydroxybenzoic acid and its derivatives, vitamin E and its derivatives, α-hydroxy acid, AMP (adenosine monophosphate, adenosine monophosphate), t-cycloamino acid One or more selected from derivatives, sohakuhi extract, chamomile extract, hydrolyzate of rice bran extract, yukinoshita extract and white sardine extract or hydrolyzate thereof can be mentioned.

Examples of the succinic acid derivative include succinic acid esters such as succinic acid monobutyrate, succinic acid monocaplate, succinic acid monopalmitate, and succinic acid dibutyrate, succinic acid ethers, and succinic acid sugar derivatives such as succinic acid glucoside. As the ascorbic acid derivative, for example, L-ascorbic acid-2-phosphate ester sodium, L-ascorbic acid-2-phosphate ester magnesium, L-ascorbic acid-2-sulfate ester sodium, L-ascorbic acid- Ascorbic acid ester salts such as 2-sulfate magnesium, L-ascorbic acid-2-glucoside, L-ascorbic acid-5-glucoside, ascorvirtocopheryl maleic acid, ascorvirtocopheryl phosphate K, myristyl 3-glyceryl ascorbic acid, Ascorbic acid sugar derivatives such as caprylyl 2-glyceryl ascorbic acid, 6-position acylated products of these ascorbic acid sugar derivatives (acyl groups are hexanoyl group, octanoyl group, decanoyle group, etc.), L-ascorbic acid tetraisopalmitic acid ester, L. -L-ascorbic acid tetra fatty acid esters such as ascorbic acid tetralauric acid ester, 3-O-ethylascorbic acid, L-ascorbic acid-2-phosphate-6-O-palmitate sodium, glyceryl ascorbic acid or its acyl Glucerin ascorbic acid derivatives such as chemical derivatives, bisglyceryl ascorbic acid, aminopropyl L-ascorbic acid phosphate, hyaluronic acid derivatives of L-ascorbic acid, 3-OD lactose-L-ascorbic acid, isostearyl ascorbyl phosphate. As the hydroquinone derivative, albutin (hydroquinone-β-D-glucopyranoside), α-arbutin (hydroquinone-α-D-glucopyranoside) and the like are used, and as the tranexamic acid derivative, tranexamic acid ester (for example, tranexamic acid lauryl ester) is used. , Tranexamic acid hexadecyl ester, tranexamic acid cetyl ester or a salt thereof), an amide form of tranexamic acid (for example, tranexamic acid methylamide) and the like, and examples of the resorcinol derivative include 4-n-butylresorcinol and 4-isoamyl. Examples of the 2,5-dihydroxybenzoic acid derivative such as resorcinol include 2,5-diacetoxybenzoic acid, 2-acetoxy-5-hydroxybenzoic acid, and 2-hydroxy-5-propionyl. Oxybenzoic acid and the like can be used as nicotinic acid derivatives such as nicotinic acid amide (niacinamide) and benzyl nicotinate, and α-hydroxy acids such as lactic acid, malic acid, succinic acid, citric acid and α-hydroxyoctane. There is acid etc.

Furthermore, the following components derived from animals, plants or microorganisms can be used in combination. For example, collagen or its hydrolyzate, yeast extract or hydrolyzate, lactic acid fungus culture, rice plant, abrana family plant, camellia family plant, rose family plant, button family plant, citrus family plant, hygiene plant, amamo Family plants, legumes, sardines, legumes, blue-green algae, lindo plants, perilla plants, lotus plants, squirrels, stag beetles, eggplants, stag beetles, matatabi , Kuwa family plant, Ayame family plant, Kyoko family plant, Mokusei family plant, Matatabi family plant, Kuwa family plant, Crow beetle family plant, Orchid family plant, Urushi family plant, Fukugi family plant, Valenshi family plant, Mikan family plant, Futomomo Extracts or hydrolysates or fermented products thereof of one or more plants selected from any of family plants, lily family plants, Benkeisou family plants, Hinoki family plants, Hirugao family plants and Kujikakushi family, Combu family, Mirin family And one or more seaweed extracts or hydrolyzates or fermented products thereof selected from any of the family Aosa, jellyfish (self-digested products such as water jellyfish, Echizen jellyfish, etc.), hyaluronic acid hydrolysates or fermented products, and royal jelly. Extract or hydrolyzate or fermented product thereof.

Plant-derived components of the Gramineae family include, in particular, rice leaf hydrolyzate, rice extract hydrolyzate, rice bran extract hydrolyzate, germinated brown rice hydrolyzate, fermented rice liquid, sake lees extract derived from sake, madake or Bran extract and fermented grass seeds are preferred. Further, as the Brassicaceae plant, an extract of seeds of Hakugai, Ogai or Kokugai, or a hydrolyzate or fermented product thereof is particularly preferable. In addition, green tea (Yabukita, Samidori, Asahi, Goko, Ujimidori, Kyomidori, Ujihikari, Samidori, Benifuki, etc.) and black tea (Darjeeling, Assam, Ceron, Earl, etc.) and black tea (Darjeeling, Assam, Ceron, Earl, etc.) are particularly included as ingredients derived from Theaceae plants. Gray, honey-scented black tea, etc.) are preferable. Ingredients derived from Rosaceae plants include damask rose flower extract, peach flower, leaf or immature fruit extract, apricot fruit or seed extract, strawberry flower extract, cherry blossom or leaf extract. Extracts are preferred. Further, as the component derived from a Peony plant, the root or flower of a button and the extract of a flower or root of a peony are preferable. Further, as the component derived from the Amaranthaceae plant, Hamcho extract is particularly preferable. Further, as the component derived from the Zosteraceae plant, an extract of eelgrass or Zostera japonica is particularly preferable. As the components derived from legumes, white soybean or black soybean extract or its hydrolyzate or soymilk fermented liquid, adzuki bean extract, red bean extract, and kudzu root extract are particularly preferable. Further, as the Asteraceae plant-derived component, burdock root extract, sunflower sprout extract, hagoromosou extract, Arnica extract or chamomile flower extract are particularly preferable. As the component derived from the Malvaceae plant, a fermented product of hibiscus, common hibiscus or confederate rose is preferable. As a component derived from a Gentianaceae plant, a gentiana extract is preferable. Further, as the Labiatae plant, perilla extract and beautyberry fruit extract are preferable. As the Nelumbo plant-derived component, a lotus flower or a lotus seed extract or a lotus seed fermented product is particularly preferable. As the component derived from the Cucurbitaceae plant, loofah extract is particularly preferable. As the component derived from the Araliaceae plant, an extract or fermented product of Panax ginseng is preferable. Examples of the component derived from the Solanaceae plant include extracts of eggplant (eggplant, water eggplant, rice eggplant, Kamo eggplant, etc.). As a component derived from a plant of the family Bignoniaceae, Pau d'Arco bark extract is preferable. As the component derived from the Actinidiaceae plant, an immature kiwi extract is preferable. As the Morus alba-derived component, an extract of Morus alba, an extract of mulberry fruit, an extract of fig fruit or an extract of bark are preferable. As a component derived from buckthorn family plants, jujube fruit extract is preferable. In addition, saffron is preferable as a component derived from Iridaceae plants. As a component derived from a plant of the family Bellflower, an extract or a hydrolyzate of the root of Codonopsis pisum is preferable. As the component derived from the lacquer family plant, mango fruit extract is particularly preferable. As the component derived from the Guttiferae plant, mangosteen fruit extract is particularly preferable. Further, as a component derived from a plant of the family Valenci, a cherimoya fruit extract is preferable. Ingredients derived from plants of the Rutaceae family include Satsuma mandarin, bergamot fruit extract, grapefruit or Banpeiyu fruit (including immature fruit) extract, flavonoids contained in plants such as grapefruit or hassaku orange, and sugar glycosides thereof. An extract or a mandarin orange seed extract is preferred. As the component derived from the Liliaceae plant, an extract of Madonna Lily, Yabukanzo, Casablanca, Madonna Lily, or Sasayuri is preferable. As the component derived from the Crassulaceae plant, an extract or fermented product of Rhodiola rosea (Rhodiola rosea) is particularly preferable. As the component derived from the Oleaceae plant, jasmine flower extract is particularly preferable. As the Cupressaceae plant, the juniper fruit extract is particularly preferable. As the component derived from the Myrtaceae plant, guava leaf extract is particularly preferable. As the orchidaceous plant, an extract of silane root (white and white) is particularly preferable. As the component derived from the Convolvulaceae plant, an extract of sweet potato or a fermented product thereof, an extract of sweet potato shochu lees, or a fermented product thereof is preferable. Further, asparagus (green asparagus and white asparagus) is preferable as a plant of the Asparagus family. As the component derived from kelp family seaweed, kelp extract is particularly preferable, as the component derived from kelp family seaweed, katamenkirinsai extract is preferable, and as the component derived from Ulva family seaweed, sea lettuce extract is particularly preferable. As a component derived from seaweed of the family Gloiopeltis, a gliopeltis extract is particularly preferable.

Next, the present invention will be described in more detail with reference to Production Examples, Formulation Examples, and Test Examples, but the present invention is not limited thereto. In the following, all parts mean parts by weight, and% means all parts by weight.

Production example 1. Extract preparation (1)
To 100 g of dried whole plant of unfermented rooibos (green rooibos) of rooibos (Aspalathus linearis), which is a plant of the genus Aspalathus of the legume family, add 1000 g of a mixed solvent of purified water and 1,3-butylene glycol (5: 5) to 100 g. In addition, after extraction at 80 ° C. for 2 hours, the insoluble material was removed by filtration to obtain 1025 g (solid content concentration 1.22%) of a dark brown green rooibos extract.

Production example 2. Extract preparation (2)
In the method for preparing the extract of Production Example 1, 1000 g of purified water is added instead of the mixed solvent of purified water and 1,3-butylene glycol (7: 3), and the extract is extracted at 80 ° C. for 2 hours, and then the insoluble material is filtered. To obtain 1030 g (solid content concentration 1.30%) of a dark brown green Louis Boss extract.

Test example 1. Evaluation test for expression of various genes Human dermis-derived fibroblasts NB1 RGB were prepared in 6 × 10 ⁴ cells / mL in eagle minimum essential medium containing 0.5% NCS, and 1 mL each was seeded in a 24-well plate to obtain 5% CO ₂ , The cells were cultured at 37 ° C. under saturated steam. After culturing for 24 hours, a culture solution containing the extract of Production Example 1 (the extract of Production Example 1 was added so that the final concentration of the solution was 0.1% and 0.2% with respect to the total amount of the culture solution). Was added and cultured. As a comparative control, instead of the extract of Production Example 1, a culture solution containing only a 30% 1,3-butylene glycol aqueous solution (the final concentration of the 30% 1,3-butylene glycol aqueous solution with respect to the total amount of the culture solution was 0.2. The test group (control group) to which the% adjusted) was added was set. After culturing for 24 hours, the cells were further cultured for 24 hours by irradiating with 5J ultraviolet A wave using an ultraviolet lamp (AS ONE model number SLUV-6). The cells in each test group were collected with Trizol reagent (0.5 mL manufactured by Invitrogen). To the collected cells, 100 μL of chloroform (Wako Pure Chemical Industries, Ltd.) was added, stirred and mixed, and a centrifuge (TOMY / MX). After centrifuging at 15,000 rpm at 4 ° C for 15 minutes at -160), 200 μL of only the aqueous layer was separated. 250 μL of isopropanol (manufactured by Wako Pure Chemical Industries, Ltd.) was added to the recovered aqueous layer and mixed by stirring. Then, the cells were centrifuged at 15,000 rpm and 4 ° C for 15 minutes to obtain a precipitate of total RNA. 1 mL of 75% ethanol was added to Total RNA, stirred and washed, and the conditions were 15,000 rpm and 4 ° C. Centrifuge for 15 minutes to recover the precipitate. The recovered total RNA was subjected to a reverse transcription reaction using a predetermined kit (PrimeScript RT reagent Kit with gDNA Eraser (Perfect Real Time) (manufactured by Takara Bio Co., Ltd.)) to obtain cDNA. Synthesized. Using the synthesized cDNA as a sample, use Thermal Cycler Dice (registered trademark) Real Time System Single (manufactured by Takara Bio) and SYBR (registered trademark) Premix Ex TaqTM II (Perfect Real Time) [manufactured by Takara Bio]. The expression of various genes and the expression of the internal standard β-actin gene were detected. Here, β-actin is a housekeeping gene (a gene that is commonly expressed in a certain amount in many tissues and cells). It is one of the genes that are constantly expressed and indispensable for cell maintenance and proliferation), and since the expression level is always constant, it is used as an internal standard in PCR experiments. The test results compared the expression levels of each gene in each test group when the expression level of the β-actin gene was constant. In this test system, the expression level of each gene in the control group was 100. The relative value of the expression level of the gene in other test plots was determined.

The results of Test Example 1 are shown below.
[Table 1]

As shown in Table 1 above, it was confirmed that the extract according to the present invention has a remarkably excellent effect of suppressing the expression of cytokine (IL-8, IL-1β) gene, which is an inflammatory factor of the skin. This suggests that the extract according to the present invention has an effect of suppressing skin inflammation.

Test example 2. Evaluation test of neutrophil-like cell migration activity inhibitory effect Human dermis-derived fibroblast NB1RGB was seeded at 9 × 10 ⁴ cells / hole in a 24-well microplate containing 10% NCS-containing eagle minimum essential medium, and 37 ° C. After pre-culturing under the condition of 5.0% CO ₂ for 1 day, Production Example 1 was added to the medium to a concentration of 0.1% and 0.2% (as a solution) and further cultured. The next day, 5J of ultraviolet A wave was irradiated using an ultraviolet lamp (AS ONE Corporation model number SLUV-6), and the culture supernatant 24 hours after the end of irradiation was used as a cytokine-inducing solution. At the same time, an ultraviolet non-irradiated group was set for comparison, and the collected culture supernatant was used as a cytokine uninduced solution. 500 μL of the recovered culture solution was added to the receptor side of Transwell (Corning Inc. model number 3415). Human leukemia cell line HL60 cells induced to differentiate into neutrophil-like cells by culturing in RPMI medium containing 1.25% DMSO and 10% FBS for 5 days was adjusted to 1 × 10 ⁶ cells / mL, and Transwell's cell culture 300 μL was seeded on the insert side. Two hours after seeding the differentiated HL60 cells, cells migrating to the receptor side were obtained. In addition, 200 μL of 0.5 mM EDTA / PBS (-) solution was added to a well separate from the seeding, the cell culture insert after migration was moved, and the cells were allowed to stand for 30 minutes to leave the cells remaining on the bottom surface of the cell culture insert. Got After combining the migrating cells and incorporating 2', 7'-dichlorodihydrofluorescein diacetate, the fluorescence intensity (excitation wavelength 485 nm, absorption wavelength 538 nm) was measured using a fluorescence plate reader (Fluoroscan Ascent, manufactured by Thermo Labsystems). The obtained fluorescence intensity is shown as a relative value when the cytokine non-inducible control is set to 100.

The results of Test Example 2 are shown in Table 2.
[Table 2]

As shown in Table 2, it was confirmed that the present invention has a remarkably excellent effect of suppressing neutrophil-like cell migration activity. Suppression of neutrophil-like cell migration activity means suppression of neutrophil infiltration into the inflamed site by cytokines secreted from dermal-derived fibroblasts. Therefore, the effect of the present invention suggests that neutrophils suppress infiltration into the inflamed site and that neutrophils suppress the secretion of degrading enzymes such as collagen and elastin at the inflamed site. It is suggested that the invention has an effect of improving wrinkles and tarmi.

Test example 3. IL-8 secretion suppression test Human dermis-derived fibroblasts NB1RGB were seeded in 9 × 10 ⁴ cells / hole in a 24-well microplate containing 10% NCS-containing eagle minimum essential medium, and the conditions were 37 ° C and 5.0% CO ₂ . After pre-culturing underneath for 1 day, Production Example 1 was added to the medium to a concentration of 0.1% and 0.2% (as a solution) and further cultured. The next day, 5J of ultraviolet A wave was irradiated using an ultraviolet lamp (AS ONE Corporation model number SLUV-6), and the culture supernatant 24 hours after the end of irradiation was used as an IL-8 induction solution. At the same time, an ultraviolet non-irradiated group was set for comparison, and the collected culture supernatant was used as an IL-8 uninduced solution. The amount of IL-8 in the collected culture solution was measured using an IL-8 measurement kit (manufactured by Boster Biological Technology). The obtained IL-8 amount is shown as a relative value when the uninduced control is 100.

The results of Test Example 3 are shown in Table 3.

As shown in Table 3, it was confirmed that the present invention has a remarkably excellent effect of suppressing the secretion of IL-8. This suggests that the present invention has an effect of suppressing skin inflammation.

Further, the extract of the present invention may be blended in a skin external preparation in combination with other active ingredients. For example, the extract of the present invention that suppresses the decomposition of extracellular matrix components of the skin may be used in combination with a component that promotes collagen synthesis in the dermis (for example, niacinamide).

Test example 4. Collagen synthesis promoting effect Human dermis-derived fibroblast NB1RGB was seeded in 1 × 10 ⁴ cells / hole in a 96-well microplate containing 0.5% NCS-containing eagle minimum essential medium under the conditions of 37 ° C and 5.0% CO ₂ . After pre-culturing for 1 day, a sample solution (niacinamide) prepared in advance was added to the medium in which the cells were pre-cultured so that the final concentration was 1 mM, and a test group was set. After adding the sample solution, each medium was cultured for another 5 days under the same conditions as the pre-culture conditions. Next, the medium was removed, cells were fixed with cold methanol and cold ethanol, and then stained with a 0.1% sirius red-containing saturated picric acid aqueous solution. After washing with purified water, extraction was performed with a 0.1% NaOH: methanol = 1: 1 solution, and the amount of collagen was measured at a wavelength of 540 nm using a microplate reader (Model 680, manufactured by Biorad). In the case of no sample addition (Control) in which a medium is added instead of the sample solution, the same operation as above is performed, and the relative value of the collagen amount at the time of adding each sample to the collagen amount obtained here is obtained and collagen synthesis is performed. The rate (%) was used. A similar test was also performed when 1 mM ascorbic acid phosphate magnesium salt (APM) was added as a positive control instead of the sample solution to confirm that the test system was functioning normally. ..

The results of Test Example 4 are shown in Table 4.
[Table 4]

By combining niacinamide, which has the effect of promoting dermal collagen synthesis shown in Table 4, with the extract according to the present invention, the balance between the production and decomposition of extracellular matrix components is adjusted, and a more remarkable synergistic effect of wrinkle improvement is obtained. It is suggested.

Test example 5. Wrinkle improvement test Faces of subjects (5 males in their 30s to 50s, 1 female, 6 in total) were washed and placed in a room with constant temperature and humidity (20 ° C ± 2 ° C, humidity 50% ± 5%) for 15 minutes. After acclimatization, a replica of the wrinkles on the outer corners of the eyes was collected and used as the initial replica. Then, twice a day in the morning and evening, the cream of Formulation Example 15 (Sample 1 of the present invention) and the cream of Formulation Example 15 containing no extract of Production Example 1 (Comparative Sample 1) were placed on the outer corners of the eyes on the set side. Applied. Eight weeks later, the subject's face was washed and a replica of the wrinkles at the corners of the eyes was collected. The initial replica of each subject and the replica after 8 weeks were analyzed using the replica analysis system ASA-03RXD (manufactured by Nippon Ash) for reflection. That is, the shadow of the replica when the replica was irradiated with parallel light of 30 degrees was taken into a computer as an image, and the volume of wrinkles was calculated by the average value of 6 subjects from the length of the shadow and the like.

The results of Test Example 5 are shown in Table 5.
[Table 5]

As shown in Table 5, the volume fraction of wrinkles was significantly reduced in the sample 1 of the present invention as compared with the comparative sample 1. From this, it was confirmed that the sample 1 of the present invention containing niacinamide and the extract of Production Example 1 exerts a remarkably excellent synergistic effect of improving wrinkles.

Prescription example 1. Toner [Ingredients] Part Eucalyptus oil 0.2
Polyoxyethylene (5.5) cetyl alcohol 5.0
Extract of Production Example 1 2.0
Tocopherol acetate 0.02
Dipotassium glycyrrhizinate 0.5
Monoammonium glycyrrhizinate 0.5
Stearyl glycyrrhizinate 0.05
Isopropylmethylphenol 0.1
Aligned in 0.1
D-Pantothenyl alcohol 0.1
Salicylic acid 0.5
Urea 5.0
l-Menthol 0.9
dl-menthol 0.2
1,3-butylene glycol 5.0
Sodium citrate 0.2
Methylparaben 0.1
Hinokitiol 0.003
Photosensitizer No. 201 0.002
Amount of purified water that makes 100 copies

Prescription example 2. Toner [Ingredients] Part Caprylic acid glyceryl 3.0
Polyglyceryl laurate-10 3.0
Cetanol 2.0
Behenyl alcohol 2.0
Methylparaben 0.1
Extract of Production Example 2 2.0
Ascorbic acid 3.0
Glycyrrhizic acid 0.5
β-Glycyrrhetinic acid 0.05
Tocopherol nicotinic acid ester 0.1
Resorcin 0.1
Zinc oxide 2.0
dl-camphor 0.5
Glycerin 2.0
1,3-butylene glycol 5.0
Potassium hydroxide 0.5
Amount of purified water that makes 100 copies

Prescription example 3. Toner [Ingredients] Jojoba oil 1.0
Polyoxyethylene (5.5) cetyl alcohol 5.0
Methylparaben 0.1
Extract of Production Example 1 2.0
Ascorbic acid glucoside 2.0
Tranexamic acid 2.0
ε-aminocaproic acid 0.1
Sulfur 0.2
Estradiol 0.1
Glycerin 5.0
1,3-butylene glycol 5.0
Sodium citrate 0.2
Sodium metabisulfite 0.2
d-camphor 0.1
Amount of purified water that makes 100 copies

Prescription example 4. Emulsion [Ingredients] Part Squalene 5.0
Cyclopentane Siloxane 1.0
Hexalan 3.0
Hexyldecyl isostearate 1.0
Tri (caprylic acid / capric acid) glyceryl 1.0
Polyglyceryl laurate-10 5.0
Polyglyceryl isostearate-10 5.0
Ascorbyl dipalmitate 15.0
Hydrogenated soy lecithin 1.5
Extract of Production Example 1 1.0
Ascorbic Acid Phosphate Ester Magnesium Salt 3.0
Arbutin 3.0
Potassium hydroxide 0.5
Glycerin 3.0
1,3-butylene glycol 2.0
Carboxymethyl cellulose 0.3
Xanthan gum 0.2
Snow fungus polysaccharide 0.2
Sodium hyaluronate 0.01
Tocopherol acetate 0.3
Tocopherol nicotinic acid ester 0.1
Glycyrrhizic acid 0.1
Dipotassium glycyrrhizinate 0.1
Isopropylmethylphenol 0.1
Water-soluble collagen 1.0
Hydrolyzed collagen 1.0
Sodium hyaluronate 1.0
Amount of purified water that makes 100 copies

Prescription example 5. Emulsion Emulsion was obtained in the same manner as in Formulation 4, except that 2.0 parts of L-ascorbic acid-2-glucoside was used instead of 2.0 parts of ascorbic acid phosphate magnesium salt in the components of Formulation Example 4.

Prescription example 6. Emulsion Same as in Formulation 4, except that 2.0 parts of tranexamic acid is used instead of 2.0 parts of ascorbic acid phosphate magnesium salt and 0.5 parts of potassium hydroxide among the components of Formulation Example 4. Obtained emulsion.

Prescription example 7. Emulsion A milky lotion was obtained in the same manner as in Formulation 4, except that 3.0 parts of nicotinamide was used instead of 2.0 parts of ascorbic acid phosphate magnesium salt in the components of Formulation Example 4.

Prescription example 8. Cream [Ingredients] Part Olive oil 5.0
Jojoba oil 5.0
Squalene 5.0
Hexyldecyl isostearate 5.0
Di (octyldodecyl / phytosteryl / behenyl) lauroyl glutamate 5.0
Glyceryl caprylate 1.0
Glyceryl stearate 1.0
Isostearyl glyceryl 3.0
γ-Oryzanol 0.1
Behenyl alcohol 2.0
Palmitic acid 2.5
D-Pantothenyl Alcohol 3.0
Allantoin 0.1
Riboflavin 0.01
Resorcin 0.1
Benzalkonium chloride 0.05
Urea 3.0
β-Glycyrrhetinic acid 0.1
Stearyl glycyrrhetinate 0.1
Ammonium glycyrrhizinate 0.1
Extract of Production Example 1 2.0
Lactic acid bacteria fermented rice 2.0
Hydrogenated lecithin 0.5
Hydrogenated lysolecithin 0.5
Oil-soluble Panax ginseng extract 2.0
Xanthan gum 1.0
Zinc oxide 0.5
dl-camphor 0.3
l-Menthol 0.5
Amount of purified water that makes 100 copies

Example 9. Pack [Ingredients] Part Dipropylene Glycol 5.0
Polyoxyethylene (60) Hardened Castor Oil 5.0
Cetanol 3.0
Behenyl alcohol 3.0
Allantoin 0.1
Dipotassium glycyrrhizinate 0.1
Ammonium glycyrrhizinate 0.1
β-Glycyrrhetinic acid 0.1
Stearyl glycyrrhetinate 0.1
Salicylic acid 0.1
Tocopherol acetate 0.5
Tocopherol nicotinic acid ester 0.1
D-Pantothenyl Alcohol 0.3
Resorcin 0.1
Sulfur 2.0
Estradiol 0.002
Extract of Production Example 2 1.0
Xanthan gum 2.0
Polyglyceryl myristate-6 1.0
Potassium cocoyl glutamate 1.0
Hydrogenated lecithin 3.0
Lecithin Hydroxide 3.0
Amount of purified water that makes 100 copies

Prescription example 10. Hair shampoo [Ingredients] Part Sodium Laureth Sulfate 10.0
Glyceryl monostearate 1.0
Coconut oil fatty acid diethanolamide 2.0
Polyoxyethylene (40) Hardened Castor Oil 0.5
Benzalkonium chloride 1.0
Stearyl alcohol 2.0
Behenyl alcohol 2.0
Dimethicone 3.0
Extract of Production Example 1 2.0
Allantoin 0.1
Dipotassium glycyrrhizinate 0.1
Salicylic acid 0.1
Sodium salicylate 0.1
Tocopherol acetate 0.1
Pyrithion zinc 0.3
Benzoic acid 0.2
Triclosan 0.2
Citric acid 0.1
Propylene glycol 2.0
Amount of purified water that makes 100 copies

Example 11. Hair Conditioner [Ingredients] Part Polyoxyethylene (10) Hardened Castor Oil 1.0
Distearyl dimethylammonium chloride 1.5
Stearyl trimethylammonium chloride 2.0
Glyceryl 2-ethylhexanoate 1.0
Benzalkonium chloride 1.0
Cetanol 3.0
Stearyl alcohol 1.0
Extract of Production Example 1 2.0
Allantoin 0.1
Isopropylmethylphenol 0.1
Dipotassium glycyrrhizinate 0.1
Salicylic acid 0.1
Sulfur 0.5
Haloalkane bromide isoquinolinium solution (75%) 0.06
Pyrithion zinc 0.3
Methylparaben 0.1
Triclosan 0.2
Resorcin 0.1
Amount of purified water that makes 100 copies

Prescription example 11. Cleaning cosmetics [Ingredients] Part Cocoyl glycine potassium 5.0
Glycerin 10.0
Glyceryl caprylate 1.0
Sodium lauroyl aspartate 10.0
Extract of Production Example 1 1.0
Cetanol 3.0
Myristyl Alcohol 3.0
Isopropylmethyl alcohol 0.1
Allantoin 0.1
Sulfur 0.5
Glycyrrhizic acid 0.1
Dipotassium glycyrrhizinate 0.1
Monoammonium glycyrrhizinate 0.1
β-Glycyrrhetinic acid 0.05
Stearyl glycyrrhetinate 0.1
Salicylic acid 0.2
Tocopherol acetate 0.2
Triclosan 0.1
Trichlorocarbanide 0.5
Trichlorohydroxydiphenyl ether 0.2
Concentrated benzalkonium chloride solution 50 0.2
Benzalkonium chloride 0.1
Amount of purified water that makes 100 copies

Prescription example 13. Sheet mask A sheet mask is obtained by impregnating a non-woven fabric with the following components.
[Ingredients] Extract of Production Example 1 2.0
Glycerin 3.0
1,3-butylene glycol 2.0
L-ascorbic acid 2-glucoside 2.0
Methylparaben 0.2
Citric acid 0.1
Sodium citrate 0.3
Xanthan gum 1.0
Water-soluble collagen 1.0
Sodium hyaluronate 1.0
Eelgrass extract 1.0
Rice extract hydrolyzate 1.0
Potassium hydroxide Appropriate amount Purified water 100 parts in total

Prescription example 14. Essence [Ingredients] Part Ethanol 2.0
Glycerin 5.0
1,3-butylene glycol 5.0
Methylparaben 0.1
Hyaluronic acid hydrolyzate 1.0
Lactic acid bacteria culture 1.0
Extract of Production Example 2 2.0
Citric acid 0.3
Sodium citrate 0.6
Amount of purified water that makes 100 copies

Prescription example 16. Wrinkle improvement cream [Ingredients] Part Olive oil 5.0
Squalene 5.0
Jojoba oil 5.0
Jojoba wax 1.0
Shea butter 2.0
Behenyl alcohol 1.0
Stearyl alcohol 1.5
Candelilla wax 0.5
Niacinamide 5.0
Extract of Production Example 1 0.2
Lactic acid bacteria fermented rice 3.0
Hydrogenated lecithin 2.0
Katamen Kirinsai Extract 2.0
Carboxyvinyl polymer 0.3
Sodium alginate 0.2
Glycerin 4.0
Potassium hydroxide Appropriate amount Purified water total 100 parts

Claims (2)

External skin preparation containing an extract of green rooibos. An anti-inflammatory or wrinkle-relieving external skin agent containing an extract of green rooibos.