JP2022025032A - Composition for reducing blood nicotine concentration, and method for producing the same - Google Patents

Abstract

To provide compositions for reducing blood nicotine concentration.SOLUTION: The present invention relates to a composition for reducing blood nicotine concentration that contains hydrogen-reduced water containing mineral ions, wheat sprout extract, Hovenia dulcis extract, Citrus tachibana peel extract, liquid fructose, and citric acid anhydride. When smokers drink the composition for reducing blood nicotine concentration of the present invention, the nicotine absorbed in the living body is decomposed and the health problems caused by nicotine can be solved.SELECTED DRAWING: Figure 1

Description

The present invention relates to a composition for reduction of nicotine content in blood, and more specifically, hydrogen-reduced water containing mineral ions, wheat sprout extract, hovenia dulcis extract, and kippi extract. It relates to a composition for reducing blood nicotine concentration, which comprises a substance, liquid fructose, and anhydrous citrate.

Nicotine, a toxic substance contained in tobacco and absorbed by the body, is a colorless or pale yellow alkaloid-based compound that is highly toxic and therefore has a rapid effect and is fatal to act on all organs of the human body. Hazardous substance.

Nicotine addiction is considered to be one of the most serious addictions to humans, although humans absorb nicotine primarily through the activity of smoking and feel arousal. The reason why it is difficult to quit smoking is nicotine dependence. Nicotine is a highly addictive substance, and if it is taken directly instead of smoking, even the amount of nicotine equivalent to 7 cigarettes is fatal.

On the other hand, 70-80% of nicotine is metabolized as cotinine by a two-step process and excreted by urine. The molecular formula of nicotine is C ₁₀ H ₁₄ N ₂ , the boiling point is 247 ° C, and the specific density is 1.0097. It is volatile at room temperature and oxidizes to brown when exposed to light or air.

Nicotine is also known to have an adverse effect on the human body by generating harmful active oxygen during the metabolic process of decomposition in the body after absorption. Harmful active oxygen refers to oxygen that is part of the oxygen that comes in by respiration and changes to an unstable state during the metabolic process, or oxygen ions that are released during the process of decomposing high molecular compounds such as nicotine in the liver. Point to. In addition to damaging cell membranes, harmful active oxygen reduces the function of mitochondria and interferes with the production of bioenergy, and it is known that about 90% of modern human diseases are associated with active oxygen.

In recent years, there has been increasing interest in hydrogen-reduced water as a means for removing harmful active oxygen in the body. Hydrogen-reduced water has a pH of 8.0 or higher, and has an excellent effect on removing harmful substances such as active oxygen in the body and anions such as chlorine. Hydrogen is known to have an antioxidant effect that is about 200 times higher than that of vitamin C, which is known as a representative of antioxidants. Vitamin C, which is water-soluble, can pass through water but not fat, whereas hydrogen, which can also pass through fat, is an energy production plant inside cells and is called a life power plant. It is transmitted to mitochondria. Moreover, vitamin C cannot prevent hydroxyl radical harmful active oxygen, but hydrogen also prevents the activity of the harmful active oxygen.

On the other hand, the redox potential is an index for determining the redox property of water. Water showing a negative redox potential is called reduced water and has reducing power. On the other hand, the redox potential of tap water is +500 to +750 mV, and that of well water and commercially available mineral water is 0 to +500 mV, and these are waters having oxidizing power.

Reduced water showing a negative oxidation-reduction potential has the effect of suppressing metal oxidation and food spoilage, and when taken as drinking water, active oxygen in the body, which is a causative agent of aging and diseases, is removed, and pollen. It is said that allergic diseases such as allergies, atopy and asthma, digestive system diseases such as gastrointestinal tract, and health disorders such as high blood pressure can be improved.

The reduced water can be produced by an electrolytic method. Utilizing the property that hydrogen molecules gather on the cathode side by electrolysis of water, water with a high concentration of active hydrogen on the cathode side is taken out as reduced water. The reduced water obtained by the electrolytic method is called "electrolyzed reduced water" to distinguish it from natural water having reducing properties, or "alkaline reduced water" because the water on the cathode side is alkalized. Another method for producing hydrogen-reduced water is not an electrolysis method of water, but a method of blowing compressed hydrogen gas into water to remove dissolved oxygen in the water and increase the amount of dissolved hydrogen.

On the other hand, mineral ions are also powerful antioxidants, and it has been clarified that they have an excellent effect of removing harmful active oxygen. All minerals (metal components) have an antioxidant effect that strongly adsorbs harmful active oxygen, which is an anion, and discharges it to the outside of the body while being cationized.

On the other hand, natural substances isolated from plants have long been known to be less likely to develop heart disease or cancer if fresh vegetables are consumed for the purpose of treating the disease. It is presumed to be due to the antioxidant action of specific components contained in the food to be ingested and the inhibitory action of specific enzymes that induce cancer.

Young wheat shoots (young wheat leaves) refer to young leaves harvested about 8 to 15 days after sowing wheat seeds, and have a high content of inorganic components such as calcium, magnesium and potassium for some time. , Vitamin B1 and Vitamin C are also high, so it is known as a nutritionally excellent food source (Republic of Korea Registered Patent No. 10-0538058, method for producing wheat leaf tea). It also contains functional secondary metabolites such as saponarin, Lutonarin and isovitexin, which have antioxidant, anti-inflammatory and anti-cancer functions, such as hypertension, diabetes and abnormal lipid metabolism. Since it contains many functional substances such as polycosanol, which is effective for metabolic syndrome, it is widely used as a health functional food and pharmaceutical material.

On the other hand, Hovenia dulcis is a deciduous foliage tree of the buckthorn family, and its Chinese name is 枳 ▲ wood tool ▼ child (kigushi), shiraishi tree, wood honey, genpashi, etc. And branches have been used for food and fruit liquor, and have been used regularly for medicinal purposes to remove liquor poison (Kim Tae Kyung, "Korean Plant Resources III" (1996), published by Seoul National University. Department, 72). According to the Hovenia dulcis, the fruit of Hovenia dulcis is said to dissolve alcohol poisoning and stop nausea. Traditionally, seeds are used for alcohol intoxication, sperm blood, stool disadvantage and vomiting, and fruit sickness is healthy. It is said to be effective for stomach and nourishing blood (Korea J. Poisonvest Sci, Technol. 6,469 (1999), Ju Sang-woo; Primary Color Korean Botanical Encyclopedia, Seasonal Plants in Mountains and Fields, p535 (1992)).

On the other hand, Kippi (tachibana skin) refers to the skin of mandarin oranges and is also called chenpi. Kippi is known to have an improving effect on cardiovascular diseases and metabolic diseases, an anticancer effect, an antiviral effect, and the like. In addition, hesperidin contained in Kippi, or hesperetin obtained by removing glycosides from hesperidin, strengthens capillaries, reduces permeability, lowers blood pressure, lowers blood cholesterol, and anti-platelet aggregation. It is known to have anti-inflammatory and anti-viral effects. In addition, naringin contained in Kippi or naringenin obtained by removing glycosides from naringin is known to have cholesterol-lowering and anticancer / antigastric ulcer (protection from alcohol) effects.

As mentioned above, noting that the young shoots of wheat, Hovenia dulcis and chenpi are particularly effective in promoting human health, hydrogen containing mineral ions, which is the main material, is used as a composition for removing the toxicity of nicotine. We have researched and developed the ideal blending ratio with reduced water.

Republic of Korea Published Patent No. 10-2001-0014750 (Publication date: 2001.02.26): Technology related to functional beverages using natural foods such as green tea leaves, morus alba, ginkgo, and celery. Republic of Korea Registered Patent No. 10-0024884 (Registration date: 2005.03.11): Techniques related to biopharmaceutical extracts having detoxifying action on nicotine and dioxin Republic of Korea Registered Patent No. 10-0494223 (Registration date: 2005.5.31): Techniques for complex natural compositions for detoxification of nicotine and tar Republic of Korea Registered Patent No. 10-0029117 (Published: 2012.03.26): Technique using Smilax china china extract and fermented product to promote nicotine decomposition Republic of Korea Registered Patent No. 10-1900304 (Registration date: 2018.09.13): Technology using green tea and black tea extract for nicotine detoxification Republic of Korea Registered Patent No. 10-1222455 (Registration date: 2013.01.09): Technology for producing hydrogen-reduced water Republic of Korea Registered Patent No. 10-103721 (Registration date: 2011.04.15): Technology for producing hydrogen-reduced water with abundant dissolved hydrogen concentration using a closed chamber Japan Registered Patent No. 10-2005-099933 (Registration date: 2005.10.17): Technology for producing hydrogen-reduced water Republic of Korea Published Patent No. 10-2014-0065678 (Publication date: 2014.05.30): Technique for producing a composition for reducing blood alcohol concentration or improving liver function containing a young wheat bud extract as an active ingredient.

Kyung Yun-ju, Lee Dong-hee, Promotion of transfer of nicotine to cotinine by green tea extract, Journal of Korean Society of Environmental Agriculture, 2000, 19 (2). Ju Wan Ki, Kwak Young Suk, Ju Jin Hyun Gu, How to Treat Substance Abuse, Book Publishing Shinil Trading Company, 2000. Korean J. Postarvest Sci, Technol. , 6,469 (1999). Ju Sang-woo, Primary Color Korean Botanical Encyclopedia, Seasonal Plants in Mountains and Fields, p535 (1992)). Benedet JA et. al. , J. Agric Food. Chem, 2007, 55, 5499-5504.

An object of the present invention is to develop and provide a composition for decomposing nicotine containing hydrogen-reduced water containing mineral ions, wheat sprout extract, hovenia dulcis extract, kippi extract, liquid fructose, and anhydrous citric acid. And.

The present invention comprises a hydrogen-reduced water containing copper ion, zinc ion, silver ion, selenium ion, germanium ion, wheat sprout extract, hovenia dulcis extract, and kippi extract to reduce blood nicotine concentration. The composition for use is provided.

In the composition for reducing the blood nicotine concentration of the present invention, the composition for reducing the blood nicotine concentration can preferably further contain liquid fructose and anhydrous citric acid. Here, the composition for reducing the blood nicotine concentration is preferably 94 to 98% by weight of hydrogen-reduced water containing mineral ions, 0.5 to 1.5% by weight of a young shoot extract of wheat, and 0 of a kemponashi extract. .5 to 1.5% by weight, Kippi extract 0.5 to 1.5% by weight, liquid fructose 0.25 to 0.75% by weight, and anhydrous citrate 0.25 to 0.75% by weight. It may be something.

In the composition for reducing the blood nicotine concentration of the present invention, the composition may be preferably a beverage.

In the composition for reducing the blood nicotine concentration of the present invention, the wheat sprout extract, the Hovenia dulcis extract, and the Kippi extract are each preferably an extract extracted using sake spirit as an extraction solvent, and are powdered. It may be there.

In the composition for reducing blood nicotine concentration of the present invention, the hydrogen-reduced water containing the copper ion, zinc ion, silver ion, selenium ion and germanium ion preferably has an oxidation-reduction potential of −200 mV to −500 mV. It may be a thing. Here, the hydrogen-reduced water containing the copper ion, zinc ion, silver ion, selenium ion, and germanium ion having an oxidation-reduction potential of -200 mV to -500 mV uses copper, zinc, silver, selenium, and germanium as an anode. Step (a) of electrolyzing water to produce a mineral ion aqueous solution containing copper ion, zinc ion, silver ion, selenium ion, and germanium ion; the mineral ion aqueous solution produced in the step (a) was used as raw water. It may be obtained from a process including the step (b) of pressurizing and injecting hydrogen gas, and may have an oxidation-reduction potential of −200 mV to −500 mV. Further, in the hydrogen-reduced water containing copper ion, zinc ion, silver ion, selenium ion and germanium ion having an oxidation-reduction potential of -200 mV to -500 mV, copper, zinc, silver, selenium and germanium are used as an anode. Step (a) of producing a mineral ion aqueous solution containing copper ion, zinc ion, silver ion, selenium ion, and germanium ion by electrolyzing water; the mineral ion aqueous solution produced in the step (a) is used as raw water. , Obtained from a process comprising the step of electrolysis (b'); and may have a redox potential of −200 mV to −500 mV.

Smokers drink the composition for reducing blood nicotine concentration containing hydrogen-reduced water containing the mineral ions of the present invention, wheat sprout extract, hovenia dulcis extract, kippi extract, liquid fructose, and anhydrous citric acid. In this case, the nicotine absorbed in the living body is decomposed, and the health problem caused by nicotine can be solved.

It is a figure which shows the measurement result of ALT and AST after ingestion of the beverage composition of the Example and the beverage composition of the comparative example which concerns on this invention. It is a figure which showed the liver function index after the lapse of a certain time after taking the beverage composition of the Example which concerns on this invention.

The present invention comprises a hydrogen-reduced water containing copper ion, zinc ion, silver ion, selenium ion, germanium ion, wheat sprout extract, hovenia dulcis extract, and kippi extract to reduce blood nicotine concentration. The composition for use is provided. Here, the composition for reducing the blood nicotine concentration of the present invention can preferably further contain liquid fructose and anhydrous citric acid. Further, the composition of the present invention is not necessarily limited to a specific dosage form, and may be preferably a beverage.

In the present invention, hydrogen-reduced water containing mineral ions, wheat sprout extract, hovenia dulcis extract, and kippi extract are used as main raw materials, and liquid fructose and anhydrous citric acid are contained as auxiliary components to reduce the blood nicotine concentration. When the composition of the present invention was ingested by smoking adults, a remarkably excellent ability to decompose nicotine was confirmed.

The composition for reducing the blood nicotine concentration of the present invention preferably contains 94 to 98% by weight of hydrogen-reduced water containing mineral ions, 0.5 to 1.5% by weight of a young shoot extract of wheat, and 0. It is composed of 5 to 1.5% by weight, Kippi extract 0.5 to 1.5% by weight, liquid fructose 0.25 to 0.75% by weight, and anhydrous citrate 0.25 to 0.75% by weight. It may be a thing.

In the present invention, hydrogen-reduced water containing copper ion, zinc ion, silver ion, selenium ion, and germanium ion (so-called hydrogen-reduced water containing mineral ion) is used, preferably -200 mV to -500 mV. Those having an oxidation-reduction potential may be used.

On the other hand, the method for producing hydrogen-reduced water containing copper ion, zinc ion, silver ion, selenium ion, and germanium ion of the present invention can be described separately for the steps of producing an aqueous mineral ion solution and producing hydrogen-reduced water. Is as follows.

<Step (a): Production of aqueous mineral ion solution>
This stage is a process of electrolyzing water using copper, zinc, silver, selenium, and germanium as an anode to produce a mineral ion aqueous solution containing copper ion, zinc ion, silver ion, selenium ion, and germanium ion. ..

The mineral ion aqueous solution can be produced by the following process.

First, copper, zinc, and silver are alloyed into a single plate to be used as a basic electrode plate. At this time, preferably, copper is mixed in a weight ratio of 1/6, zinc in a weight ratio of 3/6, and silver in a weight ratio of 2/6 to form an alloy. On the other hand, selenium and germanium are not alloyed with the above metals and are difficult to separate in the form of pure compounds. Therefore, it is preferable to use selenium-containing rocks and germanium-containing rocks in powder form. Preferably, 1 part by weight of rock containing selenium and 1 part by weight of rock containing germanium are placed in a titanium-coated wire mesh (iron ions do not dissociate only when coated with titanium), and the copper produced above- It adheres to the zinc-silver basic electrode plate so as to be energized, and is used as an electrode plate for producing the mineral ion aqueous solution of the present invention (hereinafter referred to as'electrode plate').

By electrolyzing the electrode plate produced from the above process as an anode, a mineral ion aqueous solution in which cations are dissociated can be produced. When the electrode plate produced in the present invention is adopted and put into purified water as (+) and (-) electrodes, respectively, and a voltage is applied, cations are dissociated from the (+) electrode metal plate. At this time, the (+) electrode metal plate is continuously worn over time, and impurities adhere to the (-) electrode. To prevent this, it is preferable to replace the electrodes at regular time intervals (preferably). It is preferable to exchange the (+) pole and the (-) pole with a relay at 10-minute intervals.) This makes it possible to maintain high electrolysis efficiency.

To explain more specifically by giving an example, copper (1/6 weight ratio), zinc (3/6 weight ratio), and silver (2/6 weight ratio) are alloyed to make 1 kg of a single basic electrode plate. , 500 g of selenium-containing rock and 500 g of germanium-containing rock are confined in a titanium-coated wire net and attached to the alloy plate so as to be energized, whereby a total of 2 kg of an electrode plate can be manufactured. When two electrode plates manufactured in this way are adopted as (+) poles and (-) poles, respectively, and placed in 500 L of purified water and a voltage is applied, cations dissociate from the (+) pole metal plate and mineral ions are formed. An aqueous solution can be produced. At this time, about 6 ppm of copper ion, about 2 ppm of zinc ion, and about 4 ppm of silver ion are dissociated into the mineral ion aqueous solution, and selenium ion is about 1,500 ppb (variable depending on the amount of selenium contained in the rock). ), Germanium ions are present in about 3,000 ppb (variable depending on the amount of germanium contained in the rock) dissociated into the mineral ion aqueous solution.

Mineral ions liberated as cations are also powerful antioxidants and exert excellent effects of removing harmful active oxygen. Mineral ions absorbed by the body are cations, and they strongly adsorb harmful active oxygen, which is an anion, and expel them out of the body. Further, since it is an electrically charged cation itself, it also has an action as a catalyst that decomposes a complex compound such as nicotine and promotes oxidation / reduction.

<Step (b) or (b'): Production of hydrogen-reduced water>
In this step, hydrogen-reduced water is produced by a process including the step (b); in which hydrogen gas is pressurized and injected using the mineral ion aqueous solution produced in the step (a) as raw water, or the hydrogen-reduced water is produced in the step (a). This is a process of producing hydrogen-reduced water by a process including a step of electrolyzing (b'); using the mineral ion aqueous solution produced in the above as raw water.

At this stage, the mineral ion aqueous solution produced in the step (a) is used as raw water to produce hydrogen-reduced water having an oxidation-reduction potential of −200 mV to −500 mV. For the production of hydrogen-reduced water, ordinary methods (electrolysis method, pressure mixing method) in the field to which this technique belongs can be used, but this is a known technique in the art, and a specific description thereof will be given. Is omitted. Preferably, a pressure mixing method is used. When using the pressure mixing method, it is preferable to pressurize hydrogen to 3 atm or more to mix.

On the other hand, in the present invention, wheat sprout extract, Hovenia dulcis extract, and Kippi extract are used, but these are extracted from wheat sprout, Hovenia dulcis, and Kippi by the extraction method usually used in the field to which the present technology belongs. It may be the one. It is preferable to use one extracted using water, alcohol or any of a mixed solvent thereof as an extraction solvent, and more preferably alcohol is used as a solvent.

Here, kippi is a synonym for chenpi and means the skin of the mature fruit of the Rutaceae Satsuma mandarin (Citrus unshiu Markovich) or a closely related plant of the same genus. The Kippi of the present invention can be used as long as it belongs to Citrus unshiu Markovich of the Rutaceae family or a closely related plant of the same genus. For example, Yuzu also belongs to the Rutaceae family and can be used.

On the other hand, Hovenia dulcis is actually used for food and medicinal purposes and can be used, but as in Production Example 4 of the present invention described later, "Hovenia dulcis leaf, xylem (branch)". It is preferable to mix and use.

Further, the'extract'used in the present invention is a method in which the young barley extract, the kemponashi extract, and the kippi extract of the present invention are distilled under reduced pressure and freeze-dried or spray-dried by a method widely known in the art. It also means that the product is produced in a powder state by an additional process such as, but it is preferably used in the powder state in the present invention.

On the other hand, the liquid fructose used in the present invention is added as a sugar in the composition of the present invention, but in addition to the liquid fructose, monosaccharides, disaccharides, polysaccharides, sugar alcohols and sugar are used as sugars. It is also possible to further add and blend an ester or the like.

On the other hand, the anhydrous citric acid used in the present invention is used as an appropriate pH adjuster or buffer in a beverage composition, but in addition to anhydrous citric acid, tartrate acid, malic acid, as a pH adjuster or buffer, Weak acids such as lactic acid and carbonic acid and their salts such as sodium citrate, ammonium citrate, sodium tartrate, sodium malate, sodium lactate, calcium lactate, sodium carbonate, sodium hydrogencarbonate, sodium hydrogenphosphate may also be used. can. These acids and salts thereof may be used alone or in combination of two or more. The blending ratio thereof is appropriately determined as long as the produced beverage maintains the appropriate pH range.

Hereinafter, the contents of the present invention will be specifically described with reference to the following production examples, examples and experimental examples. However, the scope of rights of the present invention is not limited to the following manufacturing examples, examples and experimental examples, and includes modifications of technical ideas equivalent thereto.

[Production Example 1: Production of Aqueous Mineral Ion Solution]
A single basic electrode plate 1 kg was made as an alloy containing copper (1/6 weight ratio), zinc (3/6 weight ratio), and silver (2/6 weight ratio), and 500 g of selenium-containing rock and 500 g of germanium-containing rock were made. Was confined in a titanium-coated wire mesh and attached so as to energize the above alloy plate to produce a total (total) 2 kg electrode plate.

The two electrode plates manufactured in this way are adopted as (+) poles and (-) poles, respectively, placed in 500 L of purified water (TDS 0.5 ppm or less), and DC electricity of about 10,000 volts is applied for 120 minutes. By applying it, a mineral ion aqueous solution in which cations were dissociated could be produced. Here, about 6 ppm of copper ions, about 2 ppm of zinc ions, and about 4 ppm of silver ions are dissociated into the mineral ion aqueous solution, and about 1,500 ppb of selenium ions and about 3,000 ppb of germanium ions are minerals. It was confirmed that it was dissociated into an aqueous ion solution.

By this method, a total of 500 L of an aqueous mineral ion solution was produced.

[Production Example 2: Production of'hydrogen-reduced water containing mineral ions' of the present invention]
Using 100 L of the mineral ion aqueous solution produced in Production Example 1 as raw water, the raw water and hydrogen gas at 5 atm are simultaneously pressurized and injected into a closed tank to produce 100 L of hydrogen-reduced water having an oxidation-reduction potential of −300 mV. bottom.

[Production Example 3: Production of young wheat sprout extract]
The young wheat buds harvested about 12 days after sowing (all parts except the root part of the young wheat) were dried and then powdered to produce young wheat bud powder. 150 L of fermented alcoholic beverage, which is an extraction solvent, was added to 100 kg of young wheat sprout powder, and the mixture was stirred at room temperature for 24 hours for extraction, and then filtered through Advantech's No. 2 filter paper. Then, the filtrate was freeze-dried to produce 10 kg of wheat sprout extract powder.

[Production Example 4: Production of Hovenia dulcis extract]
Hovenia dulcis powder was produced by drying the leaves and xylem (including branches and bark) and then mixing and powdering them at a weight ratio of 1: 1. 150 L of fermented alcoholic beverage, which is an extraction solvent, was added to 100 kg of Hovenia dulcis powder, and the mixture was stirred at room temperature for 24 hours for extraction, and then filtered through Advantech's No. 2 filter paper. Then, the filtrate was freeze-dried to produce 10 kg of Hovenia dulcis extract powder.

[Manufacturing Example 5: Production of Kippi Extract]
Kippi (skin of Citrus unshiu Markovich) was dried and then pulverized to produce Kippi powder. 150 L of fermented alcoholic beverage, which is an extraction solvent, was added to 100 kg of Kippi powder, and the mixture was stirred at room temperature for 24 hours for extraction, and then filtered through Advantech No. 2 filter paper. Then, the filtrate was freeze-dried to produce 10 kg of Kippi extract powder.

[Example 1: Production of a beverage composition for decomposing nicotine of the present invention]
96% by weight of hydrogen-reduced water containing mineral ions produced above, 1% by weight of wheat sprout extract powder produced above, 1% by weight of kemponashi extract powder produced above, 1% by weight of Kippi extract powder produced above. , 0.5% by weight of liquid fructose and 0.5% by weight of anhydrous citrate were mixed to produce 100 L of the beverage composition for nicotine decomposition of the present invention.

[Comparative Example 1: Production of Beverage Composition]
96% by weight of purified water, 3% by weight of wheat sprout extract powder, 0.5% by weight of liquid fructose, and 0.5% by weight of anhydrous citric acid were mixed to prepare 100 L of the beverage composition of Comparative Example 1.

[Comparative Example 2: Production of Beverage Composition]
96% by weight of purified water, 3% by weight of Hovenia dulcis extract powder, 0.5% by weight of liquid fructose, and 0.5% by weight of anhydrous citric acid were mixed to prepare 100 L of the beverage composition of Comparative Example 2.

[Comparative Example 3: Production of Beverage Composition]
96% by weight of purified water, 3% by weight of Kippi extract powder, 0.5% by weight of liquid fructose, and 0.5% by weight of anhydrous citric acid were mixed to produce 100 L of the beverage composition of Comparative Example 3.

[Experimental Example 1: Confirmation of nicotine decomposition effect of the composition of the present invention]
In this experiment, we tried to confirm the effect of the composition of the present invention on reducing the blood nicotine concentration. In order to verify the effect of nicotine decomposition of the composition of the present invention produced in Example 1, an experiment was conducted on 20 healthy males in their 20s and 30s who smoked commercially available cigarettes for 2 years or more. rice field.

The experiment was conducted from 10 am to 3 pm, and the number of participants was 5 people (15 people) each drinking 3 types of comparative examples, and the composition of the present invention produced in Example 1 was drunk. There were 5 people to do. Twenty experimenters were informed of the precautions, and the day before the experiment, they were prohibited from drinking alcohol and unreasonable work to maintain a calm state. The experiment was conducted at.

The urine collected in the above experiment was stored in a rapid freezer at 25 ° C. below zero and taken out after 24 hours to determine the cotinine content in the DBA assay (Robert et al., Clin. Chim. Acta. 165: 45-52, 1987). Was quantified using. Urine or standard solution (standard solution) was added to 2 mL test tubes in an amount of 1,000 μL each, and for the reliability of the experimental results, each experiment was performed twice. 500 μL of 4M sodium acetate (pH 4.7) buffer solution, 200 μL of 1.5 M KCN, 200 μL of 0.4 M chloroamine-T, 1,000 μL of 78 mM barbituric acid in each of the test tubes. (Barbituric acid in acetate / water (50/50.v / v)) were sequentially added / mixed. The mixture was mixed at room temperature (20 ° C.) at 100 rpm for 5 minutes, and 200 μL of 1 M sodium metabisulfite was added thereto to terminate the reaction. Absorbance was measured at 90 nm and quantified in comparison with a standard cotinine solution, and the average values of 5 subjects in each experimental group are shown in Table 2 below.

As shown in Table 2 above, the cotinine concentration was significantly increased in the urine of all the experimental groups in the group (Example) in which the composition produced in the present invention was drunk, as compared with the comparative group. Was confirmed. That is, it was confirmed that by drinking the composition produced in the present invention, nicotine in the body absorbed after smoking is converted into cotinine in a large amount and released into urine, and as a result, nicotine can be removed. ..

[Experimental Example 2: Confirmation of hepatotoxic detoxification ability of the composition of the present invention]
In this experimental example, we tried to confirm the hepatotoxic detoxification effect of the composition of the present invention. As experimental animals, 60 5-week-old mice (sprague dawley (SD) rats) were used. SD rats (BioGenemics Inc.) used as experimental animals are generally widely used species for toxicity testing and satisfy the conditions required for toxicity testing. The number of animals in each group was 15, and the experiments were divided into a total of 4 groups (Example 1, Comparative Example 1, Comparative Example 2, and Comparative Example 3).

The relevant beverage was orally administered to all experimental groups once a day for one week at a rate of 7 ml / kg body weight. Two hours after the final oral administration, a mixture of flaxseed oil and grape seed oil was administered. Hepatic toxicity was induced by intraperitoneal injection of (1: 2) at a concentration of 5 ml / kg body weight. Twenty-four hours after the induction of hepatotoxicity, the ALT (alanine aminotransfase) and AST (aspartate aminotransfase) activities in serum were measured for all groups.

ALT is an enzyme found mainly in the liver and is a suitable index capable of detecting hepatocyte damage. The ALT value increases when there is liver injury and is a useful test for detecting liver injury. ALT can be performed with AST as part of a liver function test. In the case of acute hepatitis, the ALT value increases more than 10 times compared to normal and remains high for 1 to 2 months, and returns to normal only after 3 to 6 months. In chronic hepatitis, the ALT value increases about 4 times compared to normal. ALT values can increase rapidly when blood flow to the liver is reduced or exposed to hepatotoxic drugs. The normal range of blood ALT concentration is 0-40 U / L. An increase of 100 U / L or less can be suspected to be chronic hepatitis, fatty liver, cirrhosis, liver cancer and the like. An increase of 100-500 U / L can be suspected of heart disease or muscle disease such as chronic hepatitis, alcoholic hepatitis, myocardial infarction. Increases of 500 U / L or more occur after taking drugs that induce hepatotoxicity, when acute hepatitis that has just developed or chronic hepatitis that has been inactive is activated, if heart disease progresses acutely, obtain.

AST is an enzyme mainly present in the heart and liver and is present in small amounts in the kidneys and muscles. When liver or muscle damage occurs, AST is released into the blood and the blood concentration increases. The normal range of blood AST concentration is 0-40 U / L.

The experimental results are shown in Table 3 below. As a result value, the average value of each of 15 animals was derived. As a result of the experiment, it was confirmed that the ALT and AST activities of the examples were significantly lower than those of the three comparative examples. The significantly lower ALT and AST activities compared to the three comparative cases are that the value of aminotransferase for removing the amino group, which is a hepatotoxic substance, is lowered, and the liver is restored to its original state. It is thought that this is because. From the above results, it was confirmed that the beverage composition of the present invention is useful for eliminating or improving hepatotoxicity.

[Experimental Example 3: Liver function improvement experiment of the composition of the present invention]
In this experimental example, an experiment for improving liver function of the composition of the present invention was carried out. The beverage composition produced in Example 1 was administered to 50 male patients aged 35 to 50 years with liver dysfunction 3 times a day at 200 mL for 30 days, and then the liver function-related index before and after ingestion was used. Certain GPT, GOT, and γ-GTP were measured.

The results are shown in Table 3 below. Generally, the normal ranges of GPT, GOT, and γ-GTP are 5 to 40 (U / L), 10 to 40 (U / L), and 0 to 60 (U / L), respectively. GPT, GOT, and γ-GTP were measured before and after taking the examples for 50 patients, and the average value was calculated, and the following results were derived. As a result of the experiment, it was confirmed that the beverage composition of the present invention significantly reduced the liver function index of patients with hepatic dysfunction. From the above results, it was confirmed that the beverage composition of the present invention is effective in improving liver function and preventing liver disease.

Claims (8)

A composition for reducing blood nicotine concentration, which comprises hydrogen-reduced water containing copper ion, zinc ion, silver ion, selenium ion, and germanium ion, wheat sprout extract, hovenia dulcis extract, and kippi extract. thing. The composition for reducing the blood nicotine concentration is
The composition for reducing blood nicotine concentration according to claim 1, further comprising liquid fructose and anhydrous citric acid. The composition for reducing the blood nicotine concentration is
Hydrogen-reduced water containing mineral ions 94-98% by weight, wheat sprout extract 0.5-1.5% by weight, Kemponashi extract 0.5-1.5% by weight, Kippi extract 0.5-1 The blood nicotine concentration according to claim 2, which is composed of .5% by weight, liquid fructose 0.25 to 0.75% by weight, and anhydrous citrate 0.25 to 0.75% by weight. Composition for reduction. The composition is
The composition for reducing blood nicotine concentration according to claim 1, which is a beverage. Each of the young shoot extract, Hovenia dulcis extract, and Kippi extract of wheat is
The composition for reducing blood nicotine concentration according to claim 1, which is an extract extracted using alcohol as an extraction solvent and is a powder. The hydrogen-reduced water containing the copper ion, zinc ion, silver ion, selenium ion, and germanium ion is
The composition for reducing blood nicotine concentration according to claim 1, which has an oxidation-reduction potential of −200 mV to −500 mV. The hydrogen-reduced water containing copper ion, zinc ion, silver ion, selenium ion, and germanium ion having an oxidation-reduction potential of -200 mV to -500 mV is
Step (a) of producing a mineral ion aqueous solution containing copper ion, zinc ion, silver ion, selenium ion, and germanium ion by electrolyzing water using copper, zinc, silver, selenium, and germanium as an anode;
It is obtained from a process including the step (b); in which the mineral ion aqueous solution produced in the step (a) is used as raw water and hydrogen gas is pressurized and injected, and has a redox potential of -200 mV to -500 mV. 6. The composition for reducing blood nicotine concentration according to claim 6. The hydrogen-reduced water containing copper ion, zinc ion, silver ion, selenium ion, and germanium ion having an oxidation-reduction potential of -200 mV to -500 mV is
Step (a) of producing a mineral ion aqueous solution containing copper ion, zinc ion, silver ion, selenium ion, and germanium ion by electrolyzing water using copper, zinc, silver, selenium, and germanium as an anode;
It was obtained from a process including the step (b'); in which the aqueous mineral ion solution produced in the step (a) was used as raw water and electrolyzed, and is characterized by having a redox potential of -200 mV to -500 mV. The composition for reducing the blood nicotine concentration according to claim 6.

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