KR101103576B1 - Composition for Removing Hangover and Composition for Improving Liver Function Using Bamboo Sprout and Bamboo Leaf - Google Patents

Abstract

The present invention relates to a hangover relieving composition comprising a bamboo shoot extract and bamboo leaf extract as an active ingredient and a composition for improving liver function.

Bamboo shoot, bamboo leaf, hangover, liver function, improvement

Description

Composition for Removing Hangover and Composition for Improving Liver Function Using Bamboo Sprout and Bamboo Leaf}

The present invention relates to a composition for relieving hangover using bamboo shoots and bamboo leaves and a composition for improving liver function.

The hangover phenomenon is caused by the toxicity of ethyl alcohol and / or acetaldehyde accumulated in liver cells. The toxicity of ethyl alcohol and / or acetaldehyde continues for a long time, causing metabolic disorders and so on, resulting in generalized boredom, fatigue and abdominal bloating. , May cause symptoms such as vomiting.

In normal ethyl alcohol metabolism, ethyl alcohol, which enters the body, is absorbed by the gastrointestinal or small intestine, enters the blood vessels, and is transferred to the liver, and alcohol is oxidized to acetaldehyde by alcohol dehydrogenase (alcoholase) in liver cells. Aldehydes are decomposed into acetic acid by acetaldehyde dehydrogenase (aldehyde degrading enzyme) in hepatocytes and finally decomposed into carbon dioxide and water.

On the other hand, there are aldehyde degrading enzymes, type II, which initiates oxidation even at low concentrations of acetaldehyde, and type I, which does not work unless acetaldehyde does not have a high concentration, but oriental people generally lack or lack acetaldehyde because of the type II aldehyde degrading enzyme. The oxidation of is slow and thus the toxicity of un-oxidized acetaldehyde and ethyl alcohol interferes with normal metabolism, causing various hangovers.

Acetaldehyde and ethyl alcohol are also known to cause hepatic deterioration along with obesity.

In order to alleviate such hangover and deterioration of liver function, a number of compositions prepared by combining single or mixed herbal preparations have been developed, such as Korean Patent No. 787633, Korean Patent No. 696589, and the like.

The present invention also discloses a technique that can solve the hangover phenomenon and reduced liver function by using a herbal formulation.

The technical problem of the present invention is to provide a hangover relief composition using bamboo shoots or bamboo leaves.

Another technical problem of the present invention is to provide a composition for improving liver function using bamboo shoots or bamboo leaves.

According to one aspect, the present invention relates to a hangover relief composition comprising bamboo shoot extract and / or bamboo leaf extract as an active ingredient.

As can be seen in the Examples and Experimental Examples below, the present invention, the water extract and 80% ethanol extract of bamboo shoots and bamboo leaves promote the activity of alcohol degrading enzyme (ADH) and aldehyde degrading enzyme (ALDH) This was done by confirming the reduction of blood alcohol levels.

As used herein, the term "active ingredient" alone refers to a component that can exhibit the desired activity or exhibit itself with a carrier which is inactive.

In addition, in this specification, "bamboo shoot" is meant to include everything obtained from a bamboo belonging to a plant that is classified as a taxonomic bamboo, regardless of the type of bamboo from which it is obtained. Regardless of the type of bamboo, bamboo shoots obtained from Bamboo belonging to Phyllostachys, bamboo shoots derived from bamboo belonging to Arundinaria, bamboo shoots obtained from bamboo belonging to Sasa or Pseudosasa All may be used in the present invention, whether bamboo shoots obtained from bamboo belonging to the). Nevertheless, it would be preferable if the bamboo shoots obtained from the powders, blinds, kings or cotton swabs widely consumed for food in Korea.

Also in this specification, "bamboo leaf" is understood as meaning including everything obtained from a plant classified as bamboo in plant taxonomy regardless of the kind of bamboo from which it is obtained.

In addition, in the present specification, "bamboo shoot extract" means an extract obtained by extracting bamboo shoots to be extracted with water, ethanol or a mixed solvent regardless of the extraction method. Regardless of the extraction method, it should be understood that the extraction method can be applied to any method such as cold soaking, refluxing, heating, and ultrasonic wave, as long as the bamboo shoots to be extracted are extracted through the step of dipping in the extraction solvent. Nevertheless, the "bamboo shoot extract" means preferably obtained by extracting bamboo shoots to be extracted with water or 80% ethanol (v / v) (exactly a mixture of water and ethanol). Here, the extract is meant to include a concentrated liquid extract or solid extract with the extraction solvent removed.

In addition, in the present specification, "bamboo leaf extract" may be defined the same as the "bamboo shoot extract" except that the extraction target is bamboo leaf.

On the other hand, the following experimental example shows that when the bamboo shoot 80% ethanol extract and bamboo leaf water extract is used in combination, the activity of alcohol degrading enzyme and aldehyde degrading enzyme is significantly increased, and the effect of reducing blood alcohol concentration of the test animal is also significantly increased.

Therefore, the composition for eliminating hangover of the present invention preferably contains both a mixed solvent extract of bamboo shoot water and ethanol and a water extract of bamboo leaf as an active ingredient.

Hangover composition of the present invention may include the bamboo shoot extract and / or bamboo leaf extract as an active ingredient in any amount (effective amount) as long as it can exhibit hangover activity, the usual effective amount is based on the total weight of the composition Will be determined in the range of 0.001% to 99.900% by weight. The term "effective amount" herein refers to an amount of an active ingredient that can induce prevention, amelioration, treatment of a symptom caused by a hangover, or delay in the expression of such a symptom. Such effective amounts can be determined experimentally within the range of ordinary skill in the art.

The hangover composition of the present invention, in addition to the bamboo shoot extract and / or bamboo leaf extract that is an active ingredient, may further include a known natural product known to have a hangover resolution activity to increase and enhance the hangover resolution activity. .

Such natural products include, for example, maisengyi extract (Korean Patent No. 502742), soybean extract (Korean Patent No. 552398), complex herbal extracts of palm cactus and alders, roots and huts (Korean Patent No. 608456), bean sprout powder or extracts. , Shellfish powder or extract, oyster powder or extract, hawthorn powder or extract, radish juice or extract, cucumber juice or extract, leek juice or extract, ginseng juice or extract and the like. Here, the meaning of the extract is as described with reference to the bamboo shoot extract. Such extracts may be obtained by mixing the extraction objects.

In another aspect, the present invention relates to a composition for improving liver function comprising bamboo shoot extract and / or bamboo leaf extract as an active ingredient.

As confirmed in the following Examples and Experimental Examples, bamboo shoot extract and bamboo leaf extract have the activity of glutamate pyruvate transaminase (hereinafter referred to as "GPT") and glutamate oxaloacetate transaminase (hereinafter referred to as "GOT") caused by alcohol administration. It can also be useful for improving liver function in that it is unfamiliar.

In the above, GPT and GOT are enzymes that are used as general indicators of liver dysfunction because the liver is secreted into the blood from the liver when damaged due to alcohol, obesity and the like.

As used herein, "recovering liver function" is meant to include preventing, ameliorating, treating, or delaying the onset of liver damage caused by alcohol or the like.

In the composition for restoring liver function of the present invention, the composition for relieving hangover of the present invention may be used in connection with a known natural product which can be used together with the active ingredient to increase and enhance the content of the active ingredient and the effect of restoring liver function. As described in this section, the same applies.

The hangover relieving composition of the present invention and the liver function restorative composition of the present invention (hereinafter "composition of the present invention") can be regarded as a food composition, in particular a functional drink, in a specific embodiment.

The food composition of the present invention may include sweeteners, flavoring agents, bioactive ingredients, minerals, etc. in addition to the active ingredients.

Sweeteners may be used in amounts that give the food a suitable sweet taste, and may be natural or synthetic. Preferably, natural sweeteners are used. Examples of natural sweeteners include sugar sweeteners such as corn syrup solids, honey, sucrose, fructose, lactose and maltose.

Flavoring agents can be used to enhance the taste or aroma, both natural and combined. Preferably, a natural one is used. When using natural ones, the purpose of nutritional fortification can be performed in addition to the flavor. The natural flavor may be obtained from apples, lemons, citrus fruits, grapes, strawberries, peaches, and the like, or may be obtained from green tea leaves, round leaves, jujube leaves, cinnamon, chrysanthemum leaves, jasmine and the like. Also, those obtained from ginseng (red ginseng), bamboo shoots, aloe vera, banks and the like can be used. The natural flavoring agent may be a liquid concentrate or a solid form of extract. In some cases, synthetic flavoring agents may be used, and synthetic flavoring agents may be used in the form of esters, alcohols, aldehydes, terpenes and the like.

As the physiologically active substance, catechins such as catechin, epicatechin, gallocatechin, epigallocatechin, vitamins such as retinol, ascorbic acid, tocopherol, calciferol, thiamine, riboflavin, and the like can be used.

As minerals, calcium, magnesium, chromium, cobalt, copper, fluoride, germanium, iodine, iron, lithium, magnesium, manganese, molybdenum, phosphorus, potassium, selenium, silicon, sodium, sulfur, vanadium, zinc and the like can be used.

In addition, the food composition of the present invention may contain a preservative, an emulsifier, an acidulant, a thickener, and the like, in addition to the sweetener.

Such preservatives, emulsifiers and the like are preferably added and used in very small amounts as long as the use to which they are added can be achieved. By trace amount is meant numerically expressed in the range of 0.0005% to about 0.5% by weight based on the total weight of the food composition.

Examples of preservatives that can be used include sodium sorbate, sodium sorbate, potassium sorbate, calcium benzoate, sodium benzoate, potassium benzoate, EDTA (ethylenediaminetetraacetic acid), and the like.

Emulsifiers that can be used include acacia gum, carboxymethylcellulose, xanthan gum, pectin and the like.

Examples of acidulants that may be used include lead acid, malic acid, fumaric acid, adipic acid, phosphoric acid, gluconic acid, tartaric acid, ascorbic acid, acetic acid, phosphoric acid, and the like. Such acidulant may be added so that the food composition is at an appropriate acidity for the purpose of inhibiting the growth of microorganisms in addition to the purpose of enhancing taste.

Thickeners that can be used include suspending implements, sedimenters, gel formers, swelling agents and the like.

The compositions of the present invention may also be contemplated as pharmaceutical compositions in specific embodiments.

When the composition of the present invention is identified as a pharmaceutical composition, the pharmacological effect may be understood as a hangover relief effect and liver function improvement effect.

The pharmaceutical composition of the present invention may include a pharmaceutically acceptable carrier in addition to bamboo shoot extract and / or bamboo leaf extract as an active ingredient. Such pharmaceutically acceptable carriers are commonly used in pharmaceutical preparations, such as lactose, Dextrose, sucrose, sorbitol, mannitol, starch, acacia rubber, calcium phosphate, alginate, gelatin, calcium silicate, microcrystalline cellulose, polyvinylpyrrolidone, cellulose, water, syrup, methyl cellulose, methylhydroxybenzo Ate, propylhydroxybenzoate, talc, magnesium stearate, mineral oil, and the like. The pharmaceutical compositions of the present invention may also further comprise lubricants, wetting agents, sweetening agents, flavoring agents, emulsifying agents, suspending agents, preservatives and the like as additives.

The carrier may be included in the pharmaceutical composition of the present invention in an amount of about 1% to about 99.99% by weight, preferably about 90% to about 99.99% by weight, based on the total weight thereof, and the additive is about 0.1% by weight. To about 20% by weight.

Meanwhile, the pharmaceutical composition of the present invention may be administered orally or parenterally, but is usually administered orally.

The pharmaceutical compositions of the present invention may be prepared in unit dose form or formulated into pharmaceutically acceptable carriers and / or excipients or incorporated into multi-dose containers. Formulations here may be in the form of solutions, suspensions or emulsions or include elexirs, extracts, powders, granules, tablets and the like.

The daily dose of the pharmaceutical composition of the present invention is usually 0.001 to 150 mg / kg body weight, and may be administered once or several times. However, since the dosage of the pharmaceutical composition of the present invention is determined in view of various related factors such as the route of administration, the age, sex, weight of the patient, and the severity of the patient, the dosage limits the scope of the present invention in any aspect. It should not be understood.

As described above, according to the present invention can provide a composition for relieving hangover and a composition for improving liver function using bamboo shoot extract and / or bamboo leaf extract.

Hangover composition and liver function improving composition of the present invention can be particularly utilized as a functional beverage.

Hereinafter, the present invention will be described with reference to Examples and Experimental Examples. However, the scope of the present invention is not limited by these examples and experimental examples.

<Examples> Preparation of Bamboo Shoot Extract and Bamboo Leaf Extract

Example 1 Preparation Example 1 of Bamboo Shoot Extract

500 g of the pulverized product obtained by pulverizing bamboo shoots was immersed in 5 L of 80% ethanol for about 24 hours, and then the extraction residue was removed and concentrated under reduced pressure to obtain a solid extract.

Example 2 Preparation Example 2 of Bamboo Shoot Extract

Bamboo shoot extract was prepared in the same manner as in <Example 1>, but the extraction solvent was used purified water instead of 80% ethanol.

Example 3 Preparation Example 1 of Bamboo Leaf Extract

500 g of the pulverized product obtained by pulverizing bamboo leaf (powder) was immersed in 5 L of 80% ethanol for about 24 hours, and then the extraction residue was removed.

Example 4 Preparation Example 2 of Bamboo Leaf Extract

Bamboo leaf extract was prepared in the same manner as in <Example 3>, but the extraction solvent was used purified water instead of 80% ethanol.

Experimental Example Hangover Relief Activity and Liver Function Improvement Activity

Experimental Example 1 Hangover Relief Activity Experiment

Experimental Example 1-1 Activity Determination of Alcohol Degrading Enzyme (ADH) and Aldehyde Degrading Enzyme (ALDH)

In order to confirm whether the bamboo shoot extract and bamboo leaf extract have hangover efficacy, first, it was determined whether each extract of the above example promotes the activity of ADH and ALDH.

The enzyme fractions used, namely the ADH fraction and the ALDH fraction, were dissolved in 8 ml of 0.1% bovine serum albumin solution in lyophilized S9 rat liver homogenate (MOLTOX Co., USA) and used after filtering with a 0.45 μm injection filter.

The reaction mixture for measuring the activity of ADH was 1.4 ml of distilled water, 0.75 ml of 1.0 M Tris-HCl buffer (pH 8.8), 0.3 ml of 20 mM NAD ⁺ , 0.3 ml of ethanol, and the sample (the above <Example 1> to <Example 4). Each extract prepared in the above) 0.1g (the mixture of the extract of <Example 1> and the extract of <Example 4> is a mixture of the same amount), 0.15ml of the enzyme fraction was prepared in a cuvette.

The reaction mixture for measuring the activity of ALDH was 2.1 ml of distilled water, 0.3 ml of 1.0M Tris-HCl buffer (pH 8.0), 0.1 ml of 20 mM NAD +, 0.1 ml of 1.0M acetaldehyde, 0.1 ml of 3.0M KCl, 0.33. 0.1 ml of M 2-mercaptoethanol (sample extract (each extract prepared in <Example 1> to <Example 4)) 0.1g (extract of <Example 1> and extract of <Example 4>) Is a mixture of the same amount), 0.15 ml of a small fraction was put into a cuvette.

Enzyme activity was measured for 5 minutes at 30 ° C., and then the NADH production rate from NAD ⁺ was measured using an absorbance at 340 nm. The results are shown in <Table 1>.

Activity measurement results of ADH and ALDH sample ADH activity (%) ALDH Activity (%) Control group 1.0 ± 0.14 1.0 ± 0.09 Extract of <Example 1> 2.24 ± 0.13 ** 2.43 ± 0.15 ** Extract of <Example 2> 1.57 ± 0.21 ** 1.49 ± 0.13 ** Extract of <Example 3> 1.75 ± 0.17 ** 1.92 ± 0.18 ** Extract of <Example 4> 2.13 ± 0.11 ** 2.32 ± 0.12 ** Extract of <Example 1>
+ Extract of <Example 4> 3.73 ± 0.23 ** 3.97 ± 0.07 ** * All experimental results are expressed as Mean ± SD value. ANOVA analysis was performed and significant differences were verified by Duncan test. * Is p <0.05 and ** is p <0.01.

The results of Table 1 show that both bamboo shoot extract and bamboo leaf extract promote the activity of ADH and ALDH. In particular, the 80% ethanol extracts of bamboo shoots and the water extracts of bamboo shoots have a high degree of promoting ADH and ALDH activity, and when the two extracts are mixed, the degree of promoting ADH and ALDH activity is significantly increased.

Experimental Example 1-2 Measurement of Alcohol Concentration

Six-week-old SD male rats were purchased from Daehan Biolink Co., Ltd. (Seoul, Republic of Korea), and were preliminarily bred while feeding solid feed and water for one week. Three rats were used in the experiment. The breeding environment maintained a temperature of 23 ± 1 ° C., a humidity of 50 ± 5%, and a 12 hour light and dark cycle.

First, oral administration of a sample (distilled water (control), an extract of each example), and then 30 minutes and 30% ethanol (v / v) was orally administered (7g / kg). After 1 hour, 3 hours and 5 hours after the ethanol administration, blood was collected from the vein of the experimental animal and treated with EDTA, and the blood alcohol concentration was measured using an alcohol measuring kit.

The results are shown in Table 2 below with the mean value of three animals (MEAN ± SE).

Measurement result of blood alcohol concentration (%) sample 1 hours 3 hours 5 hours Control group 0.125 ± 0.016 0.129 ± 0.023 0.127 ± 0.028 Extract of Example 1 (1.0 mg / kg) 0.064 ± 0.025 0.052 ± 0.019 0.040 ± 0.015 Example 2 extract (1.0 mg / kg) 0.088 ± 0.017 0.077 ± 0.016 0.071 ± 0.021 Extract of Example 3 (1.0 mg / kg) 0.084 ± 0.014 0.072 ± 0.009 0.063 ± 0.017 Example 4 extract (1.0 mg / kg) 0.059 ± 0.013 0.047 ± 0.007 0.039 ± 0.011 Extract of Example 1 (0.5 mg / kg)
+ Extract of Example 4 (0.5 mg / kg) 0.057 ± 0.013 0.032 ± 0.011 0.013 ± 0.009

The results in Table 2 also show results similar to those in Table 1. That is, all the extracts had a blood alcohol-lowering activity, but especially 80% ethanol extracts in bamboo shoots and water extracts in bamboo shoots had high blood alcohol concentrations. In addition, when 80% ethanol extract of bamboo shoot and water extract of bamboo leaf are mixed and administered, the activity is significantly increased.

Experimental Example 2 Liver Damage Protection Activity Experiment

It was examined whether each of the extracts have a liver damage protection activity caused by alcohol intake. Six-week-old SD male rats (Daehan Biolink, Korea) were taken as one experimental group, and the negative control group was not administered alcohol and the beverage of the above preparation, and the positive control group was 20% alcohol (7 g for 7 days). / kg / day) oral administration, 20% alcohol (7g / kg / day) oral administration of the extract (1.0mg / kg / day) of each example in the experimental group for 7 days. It was.

After 7 days, plasma was separated and GPT and GOT were measured using an analysis kit (Youngdong Pharm., Korea), and the experimental results are shown in Table 3 below.

Activity of GPT and GOT sample GPT (IU / l) GOT (IU / l) Control group 36.42 ± 0.21 74.27 ± 0.17 Alcohol group 68.92 ± 0.15 125.63 ± 0.16 Extract of <Example 1> 41.45 ± 0.19 81.47 ± 0.12 Extract of <Example 2> 51.83 ± 0.14 92.05 ± 0.27 Extract of <Example 3> 53.31 ± 0.17 87.64 ± 0.29 Extract of <Example 4> 43.47 ± 0.25 75.85 ± 0.39  * All experimental results are expressed as Mean ± SD value.

The results in Table 3 show that all extracts of the examples of the present invention have liver damage protective activity following alcohol damage.

Claims (9)

Bamboo shoot extract and bamboo leaf extract both include as an active ingredient, The bamboo shoot extract is obtained by extracting bamboo shoots with a mixed solvent of water and ethanol, The bamboo leaf extract is a hangover composition, characterized in that obtained by extracting the bamboo leaf with water. delete delete delete The method of claim 1, The composition for hangover relief, characterized in that the drink. delete delete delete delete