KR20110027149A - Food composition for anti-stress and skin antiaging using an extract of horse plancenta - Google Patents

Abstract

PURPOSE: An anti-stress and skin anti-aging food composition using a horse placenta extract is provided to use the horse placenta extract obtained by processing with protease. CONSTITUTION: An anti-stress and skin anti-aging food composition contains either a horse placenta extract or a crinum asiaticum extract as an active ingredient. The horse placenta extract is obtained by hydrolyzing horse placenta with papain. The crinum asiaticum extract is obtained by dipping crinum asiaticum leaves inside a mixed solution of water and ethanol. The food composition is a beverage.

Description

Food composition for anti-stress and skin antiaging using an extract of horse plancenta}

The present invention relates to an antistress and anti skin aging food composition using horse placenta extract.

Stress is a nonspecific systemic syndrome that appears in the body in response to an external stimulus.

This response to stress causes various symptoms in the human body, such as fatigue, insomnia, skin roughness (skin aging), and migraine, indigestion, hair loss, stomach ulcers, and the like.

Conventional techniques capable of relieving such stress include Korean Patent Laid-Open No. 2001-0109607, which discloses Echo extract, Magnolia extract, Green tea extract, etc. as an active ingredient of anti-stress, Prickly Pear Extract, Tofu Extract, Valerian Extract, etc. Korean Unexamined Patent Publication No. 1997-0064445 disclosed.

The present invention also discloses a food composition having antistress agent activity and anti-skin ageing activity using horse placenta extract.

An object of the present invention is to provide an antistress food composition using a horse placenta extract.

Another technical problem of the present invention is to provide an anti-skin aging food composition using a horse placenta extract.

In order to solve the above technical problem, the present inventors prepared a horse placenta extract by the treatment of protease and prepared a drink using the same, and then performed a clinical test by tasting it, and as a result, most participants feel fatigue Eliminates, reduces insomnia and improves skin roughness. In particular, the activity of horse placenta extract obtained by the treatment of protease was higher than that of horse placenta extract obtained by acid hydrolysis.

In addition, the present inventors have improved the anti-stress effect and anti-skin aging effect when the moon extract extract was used in combination with the horse placenta extract obtained by the treatment of protease, and the extract was also the anti-stress effect itself. It can be seen that it has an anti-skin aging effect.

The present invention has been completed based on these experimental results.

In one aspect, the present invention is (1) horse placenta extract obtained by the treatment of protease and / or (II) moonju egg leaves obtained by immersing in the water, ethanol or a mixed solvent thereof as an active ingredient It can be seen as an anti-stress food composition comprising, in another aspect, (I) horse placenta extract obtained by the treatment of protease and / or (II) moonju egg leaves are immersed in water, ethanol or a mixed solvent thereof It can be grasped | ascertained by the anti-aging food composition containing the obtained moonju egg extract as an active ingredient.

The horse placenta extract used as an active ingredient in the antistress agent food composition and the anti-aging food composition of the present invention (hereinafter, the "food composition of the present invention") refers to a horse placenta obtained by treating and hydrolyzing the protease. Such extract may be in the form of purified form by filtration or in the form of a concentrated liquid or solid form from which the reaction solvent is removed.

In order to achieve a sufficient hydrolytic effect, the protease is preferably added at least 0.5 parts by weight, in particular at least 2 parts by weight, based on 100 parts by weight of the horse's placenta. Specifically, the optimal amount of addition will be determined depending on the protease used. The reaction temperature at the time of hydrolysis may be selected to maximize the activity of the protease used. Usually it will be selected within the range of 35 ° C to 55 ° C. If the reaction temperature is too high, there may be a problem of inactivation of the enzyme, if it is too low, the efficiency of hydrolysis may be reduced. Proteases may be any of microorganisms, plants or animals, and there is no particular limitation, but papain, pepsin, trypsin and the like will mainly be used.

The following example shows that the horse placenta extract obtained by acid hydrolysis has lower amino acid content and lower antistress and anti-aging effects than horse placenta extract obtained by treatment with protease. It is presumed that this is because various amino acids are decomposed during acid hydrolysis or various bioactive substances such as uronic acid, glycosaminoglycan, sialic acid and epithelial cell promoters are inactivated.

Moonjuran leaf extract included as an active ingredient in the food composition of the present invention has the effect of enhancing its antistress activity when mixed with horse placenta extract in addition to itself having antistress activity. Therefore, it is preferable that the anti-stress composition of the present invention also includes a munjoran leaf extract in addition to the horse placenta extract obtained by the treatment of protease.

Moonjuran leaf extract refers to an extract obtained by immersing the moonjuran leaf to be extracted in water, ethanol or a mixed solvent of the extraction solvent, there is no particular limitation on the extraction method. Therefore, as the extraction method, any method such as heating, ultrasonication, stirring, or the like may be applied. Moonjuran leaf extract may also be purified form by filtration or in the form of a concentrated liquid or solid form in which the extraction solvent is removed. The leaves of Munjuran have been known to have effects such as analgesic, detoxification, and seedling.

In the present specification, the term "active ingredient" means a component that can exhibit activity alone or in combination with a carrier which is not active in itself.

In addition, in the present specification, the "anti-stress" refers to the reduction, prevention or elimination of fatigue and / or insomnia among the symptoms of the body caused by stress.

In addition, in the present specification, the "anti-skin aging" means the reduction, prevention or removal of skin roughness among the symptoms of the body caused by stress.

The content of the active ingredient included in the food composition of the present invention may be included in any amount according to the type of food. It will typically be included in the range of 0.5% to 35.0% by weight based on the total weight of the food composition.

In a specific embodiment, the food composition of the present invention may be commercialized with sausage, bread, chocolate, candy, snacks, confectionery, pizza, ramen, other noodles, gums, ice creams, beverages, tea, non-fatting agents, and the like. Is when it is commercialized as a beverage.

The food composition of the present invention may include sweeteners, flavoring agents, bioactive ingredients, minerals, etc. in addition to the active ingredients.

Sweeteners may be used in amounts that give the food a suitable sweet taste, and may be natural or synthetic. Preferably, natural sweeteners are used. Examples of natural sweeteners include sugar sweeteners such as corn syrup solids, honey, sucrose, fructose, lactose and maltose.

Flavoring agents can be used to enhance the taste or aroma, both natural and synthetic. Preferably, a natural one is used. When using natural ones, the purpose of nutritional fortification can be performed in addition to the flavor. The natural flavor may be obtained from apples, lemons, citrus fruits, grapes, strawberries, peaches, and the like, or may be obtained from green tea leaves, round leaves, jujube leaves, cinnamon, chrysanthemum leaves, jasmine and the like. In addition, ginseng (red ginseng), bamboo shoots, aloe vera, ginkgo and the like can be used. The natural flavoring agent may be a liquid concentrate or a solid form of extract. In some cases, synthetic flavoring agents may be used, and synthetic flavoring agents may be used in the form of esters, alcohols, aldehydes, terpenes and the like.

As the physiologically active substance, catechins such as catechin, epicatechin, gallocatechin, epigallocatechin, vitamins such as retinol, ascorbic acid, tocopherol, calciferol, thiamine, riboflavin, and the like can be used.

As minerals, calcium, magnesium, chromium, cobalt, copper, fluoride, germanium, iodine, iron, lithium, magnesium, manganese, molybdenum, phosphorus, potassium, selenium, silicon, sodium, sulfur, vanadium, zinc and the like can be used.

In addition, the food composition of the present invention may contain a preservative, an emulsifier, an acidulant, a thickener, and the like, in addition to the sweetener.

Such preservatives, emulsifiers and the like are preferably added and used in very small amounts as long as the use to which they are added can be achieved. "Ultra trace" means numerically expressed in the range from 0.0005% to about 0.5% by weight based on the total weight of the food composition.

Examples of preservatives that can be used include sodium sorbate, sodium sorbate, potassium sorbate, calcium benzoate, sodium benzoate, potassium benzoate, EDTA (ethylenediaminetetraacetic acid), and the like.

Emulsifiers that can be used include acacia gum, carboxymethylcellulose, xanthan gum, pectin and the like.

Examples of acidulants that may be used include lead acid, malic acid, fumaric acid, adipic acid, phosphoric acid, gluconic acid, tartaric acid, ascorbic acid, acetic acid, phosphoric acid, and the like. Such acidulant may be added so that the food composition is at an appropriate acidity for the purpose of inhibiting the growth of microorganisms in addition to the purpose of enhancing taste.

Thickeners that can be used include suspending implements, sedimenters, gel formers, swelling agents and the like.

In addition, the herbal composition may be added to the food composition of the present invention to improve flavor and palatability and to add other functionalities (such as prevention of arthritis or osteoporosis), which may be added as a medicinal herb extract, soybean extract, antler extract, safflower Phosphorus extract, Tosa extract, Sukjihwang extract, Tortoiseshell extract, Cornus extract, Goji berry extract, Licorice extract, Angelica extract, Root extract, Gangjinhyang extract, Haphwanpi extract, Sandugeun extract, Cultivated extract, Red ginseng extract may be exemplified. .

As described above, according to the present invention, it is possible to provide an antistress food composition and an anti-skinning food composition using a horse placenta extract obtained by the treatment of protease.

The food composition of the present invention can be used in particular as a beverage.

Hereinafter, the present invention will be described with reference to Examples, Comparative Examples, and Experimental Examples. However, the scope of the present invention is not limited to these examples, comparative examples and experimental examples.

<Examples> Preparation of Horse Placenta Extract and Enzyme Processing

Example 1 Preparation of Horse Placenta Extract

The frozen horse placenta was thawed to remove the amnion and cord from the placenta, and only placental chorionic tissue was collected and washed several times with water to remove blood. Next, the placental chorionic tissue was finely chopped, and 1 kg of chorionic tissue, 2 L of distilled water and 50 g of protease (papaine, Sigma-Aldich) were added and reacted with stirring at 55 ° C. for 16 hours in a constant temperature water bath. Next, the enzyme reaction was stopped by heating at 95 ° C. for 30 minutes, filtered through a 5 μm filter, the filtrate was concentrated under reduced pressure, water was evaporated, 1% (w / w) activated carbon was added thereto, stirred for 20 minutes, and 5 μm. Filtration again with filter. After filtering with a 0.45㎛ filter lyophilized to prepare a solid horse placenta extract.

Example 2 Preparation of Moonjuran Extract

2L of 80% (v / v) ethanol was added to 500 g of munjoran leaf pulverized, and extracted with stirring for 48 hours at room temperature, filtered through a 5㎛ filter and lyophilized to prepare a solid munjuran leaf extract.

Comparative Example Preparation of Horse Placenta Extracts by Acid Hydrolysis

The frozen horse placenta was thawed to remove the amnion and cord from the placenta, and only placental chorionic tissue was collected and washed several times with water to remove blood. Next, the placental chorionic tissue was finely chopped and hydrolyzed for 5 hours by adding 500 ml of 3N HCl to 1 kg of the chorionic tissue. The hydrolyzed product was filtered through a 5 μm filter, concentrated under reduced pressure, pH was adjusted to 7, and then 1% (w / w) of activated carbon was added thereto, stirred for 20 minutes, and filtered again using a 5 μm filter. After filtering with a 0.45㎛ filter lyophilized to prepare a solid horse placenta extract.

Experimental Example Amino Acid Constituents and Antistress and Anti-Skin Aging Tests of Horse Placenta Extracts

Experimental Example 1 Analysis of Amino Acid Components of Horse Placenta Extract

The amino acid content of the horse placenta extract by treatment of the protease obtained in <Example 1> and the horse placenta extract by acid hydrolysis obtained in the comparative example were analyzed.

Amino acid analysis was performed by AccQ-Tag amino acid analysis method (Waters). Analytical conditions were detected by HPLC (Waters 2695, USA) and the column was detected by a fluorescence detector (EX: 250 nm, EM: 395 nm) using AccQ-Tag (3.9 × 150 mm).

The results are shown in Table 1 below.

The results in Table 1 show that the amino acid content of the horse placenta extract obtained by enzymatic treatment of horse placenta increased significantly compared to horse placenta extract obtained by acid hydrolysis of horse placenta.

Experimental Example 2 Antistress Effect and Anti-Aging Effect Measurement Experiment

The horse placenta extract and Moonjuran extract obtained in Examples and Comparative Examples were clinically measured for antistress effect (improving fatigue and insomnia) and anti-skin aging (improving skin roughness).

First, a beverage containing the horse placenta extract and Moonjuran extract of Examples or Comparative Examples for the clinical test was prepared as shown in Table 2 below.

Preparation of Drinks Containing Equine Placenta Extract and Moonjulan Extract division Preparation Example 1 Production Example 2 Production Example 3 Comparative Production Example 5.0 g extract of <Example 1> o - o - 4.0 g extract of <Example 2> - o o - 5.0 g of extract of <Comparative Example> - - - o 25 ml of honey o o o o 5 ml of licorice extract o o o o 5g lemon flavor o o o o 0.5 g sodium benzoate o o o o 200 ml of refined liquor o o o o  o: contained,-: not contained

 Thirty participants were divided into 1 group of 120 participants, and 40 bottles were given to each participant. Twice a day, morning and evening, were taken before meals for 20 days.

The improvement degree was a perfect score of 7 points, 4 points or less was no effect, 4 points-6 points were somewhat effective, 6 points or more were classified as having an excellent effect.

The test results are shown in Tables 3 to 6 below.

Antistress and Anti-Skin Aging Effect of <Preparation Example 1> Participants Excellent effect Somewhat effective no effect Fatigue 10 One 8 One Blows 10 4 5 One Rough skin 10 6 2 2

Antistress and Anti-Skin Aging Effect of <Preparation Example 2> Participants Excellent effect Somewhat effective no effect Fatigue 10 0 8 2 Blows 10 One 7 2 Rough skin 10 2 7 One

Antistress and Anti-Skin Aging Effect of <Preparation Example 3> Participants Excellent effect Somewhat effective no effect Fatigue 10 7 3 0 Blows 10 7 2 One Rough skin 10 6 3 One

Antistress and Anti-Skin Aging Effect of <Comparative Preparation Example> Participants Excellent effect Somewhat effective no effect Fatigue 10 2 2 6 Blows 10 0 5 5 Rough skin 10 2 2 6

 Overall, the protease-treated horse placenta extract showed a higher effect than the acid hydrolyzed horse placenta extract.

Moonjuran extract also showed anti-stress and anti-aging effects. In particular, the anti-stress and anti-aging effects were the highest in beverages containing both placenta extract and moon juran extract.

Claims (8)

An antistress food composition comprising one or more extracts of the following (I) and (II) as an active ingredient. (Ⅰ) an extract obtained by hydrolyzing horse placenta with protease, (II) An extract obtained by immersing the leaves of moonju in water, ethanol or a mixed solvent thereof. The method of claim 1, The extract of (I) is an anti-stress food composition, characterized in that the extract obtained by hydrolyzing the horse placenta with papain. The method of claim 1, The extract of the above (II) is an anti-stress food composition, characterized in that the extract obtained by immersing the moonjuran leaves in a mixed solvent of water and ethanol. The method according to any one of claims 1 to 3, The food composition is a food composition, characterized in that the beverage. An anti-skinning food composition comprising one or more extracts of the following (I) and (II) as an active ingredient. (Ⅰ) an extract obtained by hydrolyzing horse placenta with protease, (II) An extract obtained by immersing the leaves of moonju in water, ethanol or a mixed solvent thereof. The method of claim 5, The extract of (I) is an anti-skin aging food composition, characterized in that the extract obtained by hydrolyzing the horse placenta with papain. The method of claim 5, The extract of the above (II) is an anti-skin-aging food composition, characterized in that the extract obtained by immersing the leaves of moonjuran in a mixed solvent of water and ethanol. The method according to any one of claims 5 to 7, The food composition is a food composition, characterized in that the beverage.