JP2021526832A - 多分子ルシフェラーゼ - Google Patents

多分子ルシフェラーゼ Download PDF

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Publication number
JP2021526832A
JP2021526832A JP2020569121A JP2020569121A JP2021526832A JP 2021526832 A JP2021526832 A JP 2021526832A JP 2020569121 A JP2020569121 A JP 2020569121A JP 2020569121 A JP2020569121 A JP 2020569121A JP 2021526832 A JP2021526832 A JP 2021526832A
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Japan
Prior art keywords
seq
protein
peptide
polypeptide
amino acid
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Pending
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JP2020569121A
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English (en)
Japanese (ja)
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JPWO2019241438A5 (enExample
Inventor
メアリー ホール
ランス ピー エンセル
マイケル キロラン
キース ウッド
トーマス スミス
バージニア キンケイド
トーマス カークランド
メラニー ダート
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Promega Corp
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Promega Corp
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Publication date
Application filed by Promega Corp filed Critical Promega Corp
Publication of JP2021526832A publication Critical patent/JP2021526832A/ja
Publication of JPWO2019241438A5 publication Critical patent/JPWO2019241438A5/ja
Priority to JP2024005285A priority Critical patent/JP7774084B2/ja
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/66—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving luciferase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0069—Oxidoreductases (1.) acting on single donors with incorporation of molecular oxygen, i.e. oxygenases (1.13)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y113/00—Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13)
    • C12Y113/12—Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13) with incorporation of one atom of oxygen (internal monooxygenases or internal mixed function oxidases)(1.13.12)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y113/00—Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13)
    • C12Y113/12—Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13) with incorporation of one atom of oxygen (internal monooxygenases or internal mixed function oxidases)(1.13.12)
    • C12Y113/12005—Renilla-luciferin 2-monooxygenase (1.13.12.5), i.e. renilla-luciferase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
    • G01N21/76—Chemiluminescence; Bioluminescence
    • G01N21/763—Bioluminescence
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6845—Methods of identifying protein-protein interactions in protein mixtures

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Plasma & Fusion (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
JP2020569121A 2018-06-12 2019-06-12 多分子ルシフェラーゼ Pending JP2021526832A (ja)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2024005285A JP7774084B2 (ja) 2018-06-12 2024-01-17 多分子ルシフェラーゼ

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201862684014P 2018-06-12 2018-06-12
US62/684,014 2018-06-12
PCT/US2019/036844 WO2019241438A2 (en) 2018-06-12 2019-06-12 Multipartite luciferase

Related Child Applications (1)

Application Number Title Priority Date Filing Date
JP2024005285A Division JP7774084B2 (ja) 2018-06-12 2024-01-17 多分子ルシフェラーゼ

Publications (2)

Publication Number Publication Date
JP2021526832A true JP2021526832A (ja) 2021-10-11
JPWO2019241438A5 JPWO2019241438A5 (enExample) 2022-06-17

Family

ID=68842790

Family Applications (2)

Application Number Title Priority Date Filing Date
JP2020569121A Pending JP2021526832A (ja) 2018-06-12 2019-06-12 多分子ルシフェラーゼ
JP2024005285A Active JP7774084B2 (ja) 2018-06-12 2024-01-17 多分子ルシフェラーゼ

Family Applications After (1)

Application Number Title Priority Date Filing Date
JP2024005285A Active JP7774084B2 (ja) 2018-06-12 2024-01-17 多分子ルシフェラーゼ

Country Status (5)

Country Link
US (1) US20200270586A1 (enExample)
EP (1) EP3807419A4 (enExample)
JP (2) JP2021526832A (enExample)
CN (1) CN112567028A (enExample)
WO (1) WO2019241438A2 (enExample)

Families Citing this family (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP7703446B2 (ja) 2018-11-28 2025-07-07 プロメガ コーポレイション 反応性ペプチド標識付け
JP7584433B2 (ja) 2019-03-20 2024-11-15 プロメガ コーポレイション 光親和性プローブ
US11360096B2 (en) * 2019-05-16 2022-06-14 Duke University Complex BRET technique for measuring biological interactions
US11591633B2 (en) * 2019-09-11 2023-02-28 Laboratory Corporation Of America Holdings Methods and systems for the rapid detection of bacteria using recombinant bacteriophage to express an indicator subunit
BR112022010153A2 (pt) 2019-11-27 2022-08-09 Promega Corp Peptídeos, ácido nucleico, polipeptídeo, composição, complexo bioluminescente, métodos, dipeptídeo, sistema e kit
EP4073512B1 (en) 2019-12-10 2025-11-12 Promega Corporation Compositions and methods for bioluminescent detection using multifunctional probes
WO2021231481A1 (en) * 2020-05-11 2021-11-18 Chan Zuckerberg Biohub, Inc. Detection assay for sars-cov-2 virus
CN116368156A (zh) * 2020-05-27 2023-06-30 华盛顿大学 基于从头设计的蛋白开关的模块化和通用化生物传感器平台
CN113461809A (zh) * 2020-07-17 2021-10-01 南京诺唯赞生物科技股份有限公司 筛选结合抑制剂和/或结合区域的方法
US20220064696A1 (en) 2020-08-28 2022-03-03 Promega Corporation Target engagement assay for ras proteins
WO2022120383A1 (en) * 2020-12-04 2022-06-09 Brigham Young University Method for detection of viral infections using split enzymes
CN112694535B (zh) * 2021-01-05 2023-03-31 重庆医科大学 用于抗体检测的多功能蛋白分子开关
US20240174992A1 (en) 2022-05-04 2024-05-30 Promega Corporation Split modified dehalogenase variants
JP2025515181A (ja) 2022-05-04 2025-05-13 プロメガ コーポレイション 伸長表面ループ領域を有する修飾デハロゲナーゼ
JP2025515179A (ja) 2022-05-04 2025-05-13 プロメガ コーポレイション 円順列置換デハロゲナーゼバリアント
CN116068198B (zh) * 2022-11-30 2024-01-09 深圳湾实验室 Ppi原位检测方法及其载体、诊断试剂、试剂盒和应用
US20240377404A1 (en) * 2023-03-24 2024-11-14 Promega Corporation Bioluminescence-triggered photocatalytic activation
CN117330755B (zh) * 2023-08-25 2024-03-26 广东省农业科学院农业生物基因研究中心 一种基于酶催化生物荧光定量检测dna-蛋白质相互作用的方法
WO2025064581A1 (en) * 2023-09-18 2025-03-27 The Brigham And Women’S Hospital, Inc. Biosensor platform for the rapid detection of analytes
CN117603322A (zh) * 2023-11-24 2024-02-27 生工生物工程（上海）股份有限公司 一种重组链球菌蛋白g、亲和层析树脂及其应用

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2017189751A1 (en) * 2016-04-26 2017-11-02 The University Of Utah Research Foundation Target-binding activated split reporter systems for analyte detection and related components and methods
JP2017533181A (ja) * 2014-09-12 2017-11-09 プロメガ コーポレイションＰｒｏｍｅｇａ Ｃｏｒｐｏｒａｔｉｏｎ 内部タンパク質タグ

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2438982A (en) * 2004-12-22 2007-12-12 Ambrx Inc Compostions containing, methods involving, and uses of non-natural amino acids and polypeptides
WO2009128056A2 (en) * 2008-04-18 2009-10-22 University College Cork - National University Of Ireland, Cork A method for screening compounds
AU2011323418B2 (en) 2010-11-02 2017-07-27 Promega Corporation Novel coelenterazine substrates and methods of use
WO2013177255A2 (en) * 2012-05-24 2013-11-28 Connecticut College Chimeric luciferases
ES2926463T3 (es) * 2013-03-15 2022-10-26 Promega Corp Activación de la bioluminiscencia mediante complementación estructural

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2017533181A (ja) * 2014-09-12 2017-11-09 プロメガ コーポレイションＰｒｏｍｅｇａ Ｃｏｒｐｏｒａｔｉｏｎ 内部タンパク質タグ
WO2017189751A1 (en) * 2016-04-26 2017-11-02 The University Of Utah Research Foundation Target-binding activated split reporter systems for analyte detection and related components and methods

Also Published As

Publication number Publication date
JP2024054136A (ja) 2024-04-16
EP3807419A2 (en) 2021-04-21
US20200270586A1 (en) 2020-08-27
WO2019241438A2 (en) 2019-12-19
EP3807419A4 (en) 2022-09-07
JP7774084B2 (ja) 2025-11-20
CN112567028A (zh) 2021-03-26

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