CN112567028A - 多部分萤光素酶 - Google Patents

多部分萤光素酶 Download PDF

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Publication number
CN112567028A
CN112567028A CN201980053139.3A CN201980053139A CN112567028A CN 112567028 A CN112567028 A CN 112567028A CN 201980053139 A CN201980053139 A CN 201980053139A CN 112567028 A CN112567028 A CN 112567028A
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Prior art keywords
seq
peptide
protein
polypeptide
complementary
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Pending
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CN201980053139.3A
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English (en)
Chinese (zh)
Inventor
M·霍尔
L·P·恩塞尔
M·基洛兰
K·伍德
T·史密斯
V·金凯德
T·科克兰德
M·达特
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Promega Corp
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Promega Corp
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Publication of CN112567028A publication Critical patent/CN112567028A/zh
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/66—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving luciferase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0069—Oxidoreductases (1.) acting on single donors with incorporation of molecular oxygen, i.e. oxygenases (1.13)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y113/00—Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13)
    • C12Y113/12—Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13) with incorporation of one atom of oxygen (internal monooxygenases or internal mixed function oxidases)(1.13.12)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y113/00—Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13)
    • C12Y113/12—Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13) with incorporation of one atom of oxygen (internal monooxygenases or internal mixed function oxidases)(1.13.12)
    • C12Y113/12005—Renilla-luciferin 2-monooxygenase (1.13.12.5), i.e. renilla-luciferase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
    • G01N21/76—Chemiluminescence; Bioluminescence
    • G01N21/763—Bioluminescence
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6845—Methods of identifying protein-protein interactions in protein mixtures

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Plasma & Fusion (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
CN201980053139.3A 2018-06-12 2019-06-12 多部分萤光素酶 Pending CN112567028A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201862684014P 2018-06-12 2018-06-12
US62/684,014 2018-06-12
PCT/US2019/036844 WO2019241438A2 (en) 2018-06-12 2019-06-12 Multipartite luciferase

Publications (1)

Publication Number Publication Date
CN112567028A true CN112567028A (zh) 2021-03-26

Family

ID=68842790

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201980053139.3A Pending CN112567028A (zh) 2018-06-12 2019-06-12 多部分萤光素酶

Country Status (5)

Country Link
US (1) US20200270586A1 (enExample)
EP (1) EP3807419A4 (enExample)
JP (2) JP2021526832A (enExample)
CN (1) CN112567028A (enExample)
WO (1) WO2019241438A2 (enExample)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN116068198A (zh) * 2022-11-30 2023-05-05 深圳湾实验室 Ppi原位检测方法及其载体、诊断试剂、试剂盒和应用
CN117330755A (zh) * 2023-08-25 2024-01-02 广东省农业科学院农业生物基因研究中心 一种基于酶催化生物荧光定量检测dna-蛋白质相互作用的方法
WO2025107775A1 (zh) * 2023-11-24 2025-05-30 生工生物工程（上海）股份有限公司 一种重组链球菌蛋白g、亲和层析树脂及其应用

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP7703446B2 (ja) 2018-11-28 2025-07-07 プロメガ コーポレイション 反応性ペプチド標識付け
JP7584433B2 (ja) 2019-03-20 2024-11-15 プロメガ コーポレイション 光親和性プローブ
US11360096B2 (en) * 2019-05-16 2022-06-14 Duke University Complex BRET technique for measuring biological interactions
US11591633B2 (en) * 2019-09-11 2023-02-28 Laboratory Corporation Of America Holdings Methods and systems for the rapid detection of bacteria using recombinant bacteriophage to express an indicator subunit
BR112022010153A2 (pt) 2019-11-27 2022-08-09 Promega Corp Peptídeos, ácido nucleico, polipeptídeo, composição, complexo bioluminescente, métodos, dipeptídeo, sistema e kit
EP4073512B1 (en) 2019-12-10 2025-11-12 Promega Corporation Compositions and methods for bioluminescent detection using multifunctional probes
WO2021231481A1 (en) * 2020-05-11 2021-11-18 Chan Zuckerberg Biohub, Inc. Detection assay for sars-cov-2 virus
CN116368156A (zh) * 2020-05-27 2023-06-30 华盛顿大学 基于从头设计的蛋白开关的模块化和通用化生物传感器平台
CN113461809A (zh) * 2020-07-17 2021-10-01 南京诺唯赞生物科技股份有限公司 筛选结合抑制剂和/或结合区域的方法
US20220064696A1 (en) 2020-08-28 2022-03-03 Promega Corporation Target engagement assay for ras proteins
WO2022120383A1 (en) * 2020-12-04 2022-06-09 Brigham Young University Method for detection of viral infections using split enzymes
CN112694535B (zh) * 2021-01-05 2023-03-31 重庆医科大学 用于抗体检测的多功能蛋白分子开关
US20240174992A1 (en) 2022-05-04 2024-05-30 Promega Corporation Split modified dehalogenase variants
JP2025515181A (ja) 2022-05-04 2025-05-13 プロメガ コーポレイション 伸長表面ループ領域を有する修飾デハロゲナーゼ
JP2025515179A (ja) 2022-05-04 2025-05-13 プロメガ コーポレイション 円順列置換デハロゲナーゼバリアント
US20240377404A1 (en) * 2023-03-24 2024-11-14 Promega Corporation Bioluminescence-triggered photocatalytic activation
WO2025064581A1 (en) * 2023-09-18 2025-03-27 The Brigham And Women’S Hospital, Inc. Biosensor platform for the rapid detection of analytes

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2009128056A2 (en) * 2008-04-18 2009-10-22 University College Cork - National University Of Ireland, Cork A method for screening compounds
WO2014151736A1 (en) * 2013-03-15 2014-09-25 Promega Corporation Activation of bioluminescence by structural complementation
US20150152395A1 (en) * 2012-05-24 2015-06-04 Connecticut College Chimeric luciferases
WO2017189751A1 (en) * 2016-04-26 2017-11-02 The University Of Utah Research Foundation Target-binding activated split reporter systems for analyte detection and related components and methods

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2438982A (en) * 2004-12-22 2007-12-12 Ambrx Inc Compostions containing, methods involving, and uses of non-natural amino acids and polypeptides
AU2011323418B2 (en) 2010-11-02 2017-07-27 Promega Corporation Novel coelenterazine substrates and methods of use
EP3191601B1 (en) * 2014-09-12 2022-11-02 Promega Corporation Internal protein tags

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2009128056A2 (en) * 2008-04-18 2009-10-22 University College Cork - National University Of Ireland, Cork A method for screening compounds
US20150152395A1 (en) * 2012-05-24 2015-06-04 Connecticut College Chimeric luciferases
WO2014151736A1 (en) * 2013-03-15 2014-09-25 Promega Corporation Activation of bioluminescence by structural complementation
US20140348747A1 (en) * 2013-03-15 2014-11-27 Promega Corporation Activation of bioluminescence by structural complementation
WO2017189751A1 (en) * 2016-04-26 2017-11-02 The University Of Utah Research Foundation Target-binding activated split reporter systems for analyte detection and related components and methods

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN116068198A (zh) * 2022-11-30 2023-05-05 深圳湾实验室 Ppi原位检测方法及其载体、诊断试剂、试剂盒和应用
CN116068198B (zh) * 2022-11-30 2024-01-09 深圳湾实验室 Ppi原位检测方法及其载体、诊断试剂、试剂盒和应用
CN117330755A (zh) * 2023-08-25 2024-01-02 广东省农业科学院农业生物基因研究中心 一种基于酶催化生物荧光定量检测dna-蛋白质相互作用的方法
CN117330755B (zh) * 2023-08-25 2024-03-26 广东省农业科学院农业生物基因研究中心 一种基于酶催化生物荧光定量检测dna-蛋白质相互作用的方法
WO2025107775A1 (zh) * 2023-11-24 2025-05-30 生工生物工程（上海）股份有限公司 一种重组链球菌蛋白g、亲和层析树脂及其应用

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Publication number Publication date
JP2024054136A (ja) 2024-04-16
EP3807419A2 (en) 2021-04-21
JP2021526832A (ja) 2021-10-11
US20200270586A1 (en) 2020-08-27
WO2019241438A2 (en) 2019-12-19
EP3807419A4 (en) 2022-09-07
JP7774084B2 (ja) 2025-11-20

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