JP2021122493A - Cryopreservation container - Google Patents

Abstract

To prevent a housing chamber housing liquid from swelling more than necessary in the case of performing cryopreservation of the liquid containing a sample derived from a living body.SOLUTION: A cell preservation bag includes: a container body 100 where a peripheral edge part of a first sheet-like member 101 and a peripheral edge part of a second sheet-like member 103 are welded as a peripheral edge welded part 111 and, in a center part excluding the peripheral edge welded part 111, a housing chamber 113 capable of housing, for example, preservation liquid suspending cells is formed; and a swelling suppression part 109a which is provided for a part of the housing chamber 113 of the container body 100 and the first sheet-like member 101 and the second sheet-like member 103 are welded in order to suppress excessive deformation of a shape of the housing chamber 113 when frozen and defrosted.SELECTED DRAWING: Figure 1

Description

The present invention relates to a cryopreservation container applied as a cell preservation bag used for cryopreservation of living tissues such as cells.

Conventionally, cryopreservation containers have been widely used for various purposes in fields such as regenerative medicine, gene therapy, and transplantation therapy. That is, cells collected from living tissue are frozen in a state of being contained in a cryopreservation container from the time they are collected until they are actually used for regenerative medicine, etc., and are stored together with the cryopreservation container (for example,). See Patent Document 1).

In the case of a general cell storage bag used for cryopreservation of cells, at the time of freezing, the cells are rapidly frozen using liquid nitrogen in a state where the preservation solution in which the cells are suspended is contained inside, and at the time of thawing. , It is gradually thawed using lukewarm warm water.

Japanese Unexamined Patent Publication No. 2015-42212

By the way, in the fields such as regenerative medicine as described above, there is a demand to shorten the freezing time and thawing time of the preservation solution as much as possible so as not to destroy the elemental structure and biological function of cells.

In order to shorten the freezing time and thawing time of the preservation solution without destroying the element structure and biological function of the cells, the sheet thickness of the sheet-like member constituting the cell preservation bag should be adjusted so that heat transfer is improved. Make it as thin as possible. The thinner the sheet-shaped member, the easier it is to transfer heat. Therefore, it is desirable that the sheet-shaped member be as thin as possible so that it can withstand use in an ultra-low temperature state.

However, if the sheet thickness is made too thin, the cell storage bag may be inflated due to a change in the volume of the storage solution or the like, and the cell storage bag may be easily deformed. Deformation of the cell storage bag causes the temperature distribution and concentration distribution of the stored storage liquid to become non-uniform, resulting in impairing the uniformity of freezing and thawing. Therefore, there is a problem that the freezing time and thawing time of the preservation solution cannot be easily shortened only by reducing the sheet thickness.

The present invention has been made in view of the above, and an object of the present invention is to prevent an unnecessarily swelling of a storage chamber containing a liquid containing a biological sample even when the sheet thickness is reduced. The present invention is to provide a cryopreservation container capable of shortening the freezing time and thawing time of the liquid.

In order to achieve the above object, in the cryopreservation container of one aspect of the present invention, the peripheral edge portion of the first sheet-shaped member and the peripheral edge portion of the second sheet-shaped member are welded to a portion other than the peripheral edge portion. , A cryopreservation container provided with a bag body in which a storage chamber capable of containing a liquid containing a living body-derived sample is formed, and the bag body has the shape of the storage chamber when the liquid is frozen and thawed. It is characterized in that an expansion suppressing portion for suppressing excessive deformation of the bag is provided.

According to the cryopreservation container of one aspect of the present invention, even when the thickness of the first sheet-shaped member and the second sheet-shaped member is reduced by providing the expansion suppressing portion, at the time of freezing or thawing. , It becomes possible to prevent the containment chamber from being deformed too much. This makes it possible to reduce the thickness of the first sheet-shaped member and the second sheet-shaped member as much as possible. Therefore, the freezing time and thawing time of the liquid containing the sample derived from the living body can be shortened.

According to the present invention, even when the sheet thickness is reduced, it is possible to prevent the storage chamber containing the liquid containing the biological sample from swelling more than necessary, and to shorten the freezing time and thawing time of the liquid. A possible cryopreservation container can be provided.

(A) to (c) are schematic views showing a configuration example of a cell storage bag to which the cryopreservation container according to the first embodiment is applied. (A) to (c) are schematic views showing an example of use of the cell storage bag shown in FIG. (A) and (b) are schematic views which show the structural example of the cell preservation bag which concerns on 2nd Embodiment. (A) and (b) are schematic views which show the structural example of the cell preservation bag which concerns on 3rd Embodiment. It is a schematic plan view which shows the structural example of the cell preservation bag which concerns on 4th Embodiment. It is a schematic plan view which shows the structural example of the cell storage bag which concerns on 5th Embodiment. (A) and (b) are schematic views showing a configuration example of a cell storage bag according to a sixth embodiment. (A) to (c) are schematic views showing an example of use of the cell storage bag shown in FIG. 7. (A) to (c) are schematic cross-sectional views showing a configuration example of a cell storage bag according to another embodiment.

Hereinafter, embodiments according to the present invention will be described with reference to the drawings. It should be noted that in each embodiment, the drawings schematically show the configuration of the cell storage bag and are different from the actual ones.

<First Embodiment>
1 (a) to 1 (c) schematically show a configuration example of a cell storage bag to which the cryopreservation container according to the first embodiment of the present invention is applied, and FIG. 1 (a). ) Is a top view, FIG. 1 (b) is a front view (plan view), and FIG. 1 (c) is a cross-sectional view taken along line 1c-1c of FIG. 1 (b). 2 (a) to 2 (c) schematically show an example of using the cell storage bag shown in FIGS. 1 (a) to 1 (c), and FIG. 2 (a) shows. 2 (b) is a front view, FIG. 2 (b) is a cross-sectional view taken along line 2b-2b of FIG. 2 (a), and FIG. 2 (c) is a cross-sectional view taken along line 2c-2c of FIG. 2 (a).

As shown in FIGS. 1 (a) to 1 (c), the cell storage bag of the present embodiment has a structure in which peripheral portions of two resin sheet-shaped members 101 and 103 are welded to each other. , The container body (bag body) 100 is provided.

More specifically, the container body 100 includes a peripheral edge welding portion 111 in which the peripheral edge portion of the first resin sheet-like member 101 and the peripheral edge portion of the second resin sheet-like member 103 are welded together. Then, as shown in FIGS. 2 (a) to 2 (c), for example, a storage liquid (liquid) 130 containing a sample derived from a living body is housed in a portion (spatial region) surrounded by the peripheral welding portion 111. A containment chamber 113 is formed. Specific examples of biological samples include cells, 3D aggregates of cells, cell sheets and their laminates, biological tissues and their disrupted products, intracellular organellas, extracellular secretions (exosomes, etc.), and two or more types of cells. 3D aggregates (organoids, etc.), cell sheets and laminates thereof.

The container body 100 has a swelling suppressing portion (expansion suppressing portion) for suppressing the shape of the storage chamber 113 from being deformed more than necessary during freezing and thawing along the insertion direction of the pipette (dropper) described later. ) 109a is provided. That is, by providing a plurality of (here, two) swelling suppressing portions 109a, the first sheet-shaped member 101 and the second sheet-shaped member 103 constituting the container body 100 are reinforced so as to be difficult to swell. ing.

Further, the container body 100 is welded between the peripheral edge portion of the first sheet-shaped member 101 and the peripheral edge portion of the second sheet-shaped member 103 so as to communicate with at least a part of the storage chamber 113. A port portion 105 is provided. The port portion 105 is for taking in and out the storage liquid 130, and includes, for example, a screw-type cap (cover portion) 107 that can be opened and closed with one hand.

In the present embodiment, for example, as shown in FIG. 2A, the two swelling suppressing portions 109a are arranged apart from each other by a distance that allows the pipette 120 to be inserted from the port portion 105. That is, in the two swelling suppressing portions 109a, for example, as shown in FIGS. 1 (b) and 1 (c), the first sheet-shaped member 101 and the second sheet-shaped member 103 are at the center of the accommodating chamber 113. At two places near the portion, each is welded so as to be parallel to each other and substantially linearly continuous while maintaining a predetermined distance.

As a result, for example, as shown in FIGS. 2 (a) to 2 (c), the pipette 120 can be inserted into the storage chamber 113 along the two swelling suppressing portions 109a, and the tip portion is accommodated. It is said that the vicinity of the deepest portion 113a of the chamber 113 can be easily reached.

Here, the EVA resin (ethylene-acetic acid) having a thickness of, for example, about 0.3 mm or less, preferably thinner than 0.2 mm, so that the first sheet-shaped member 101 and the second sheet-shaped member 103 do not have heat insulating ability. A flexible thermoplastic resin such as vinyl copolymer resin) is used. The film thickness (sheet thickness) of the first sheet-shaped member 101 and the second sheet-shaped member 103 is such that it can withstand use in an ultra-low temperature state without damaging the element structure and biological function of cells. Can be diluted.

Not limited to EVA resin, for example, a cyclic olefin polymer (COC resin) or the like can also be used.

At the time of freezing, the cell storage bag is submerged in liquid nitrogen at, for example, -196 ° C. in a state where the storage solution 130 containing a sample derived from a living body is contained in the storage chamber 113. As a result, the preservation solution 130 containing the sample derived from the living body is cryopreserved together with the cell preservation bag.

In this case, the cell storage bag has a storage solution 130 containing a biological sample having a substantially uniform temperature gradient and concentration gradient without the storage chamber 113 expanding more than necessary by the two swelling suppressing portions 109a. It can be held and frozen.

On the other hand, at the time of thawing, the cell storage bag is taken out from the liquid nitrogen, and for example, the cell storage bag is immersed in warm water whose temperature is controlled to about 37 ° C. This makes it possible to thaw the frozen storage liquid 130 in the storage chamber 113 with a substantially uniform temperature gradient and concentration gradient.

According to the present embodiment, for example, as shown in FIGS. 2A to 2C, a storage chamber 113 is required by the two swelling suppressing portions 109a during use, especially during freezing and thawing. It is possible to suppress the swelling as described above. Moreover, the two swelling suppressing portions 109a do not prevent the storage liquid 130 from being taken in and out (particularly from being taken out) by the pipette 120 at the time of thawing.

That is, in the cell storage bag according to the present embodiment, the two swelling suppressing portions 109a make it possible to keep the cross section of the storage chamber 113 as thin as possible and have a substantially uniform thickness. This makes it possible to equalize the heat transfer during freezing and thawing to the storage liquid 130, which is the contained substance, as much as possible. Therefore, the freezing time and thawing time of the storage solution 130 can be shortened without causing a temperature gradient or a concentration gradient.

In particular, by welding a part of the first sheet-shaped member 101 and the second sheet-shaped member 103 to the swelling suppressing portion 109a, a disposable cell storage bag can be provided at low cost.

In the cell storage bag according to the first embodiment, the swelling suppressing portion may be dispersed and arranged in a plurality of broken lines (not shown).

In any case, by increasing the number of the swelling suppressing portions, it is possible to make the cross section of the accommodation chamber 113 at the time of use thinner.

<Second Embodiment>
3 (a) and 3 (b) schematically show a configuration example of the cell storage bag (before use) according to the second embodiment of the present invention, and FIG. 3 (a) shows the front surface. FIG. 3 (b) is a cross-sectional view taken along the line 3b-3b of FIG. 3 (a). In FIGS. 3 (a) and 3 (b), the same or similar reference numerals are given to the same parts as the cell storage bag of the first embodiment, and detailed description thereof will be omitted.

That is, the cell storage bag of the present embodiment has, for example, a first swelling suppressing portion (first suppressing portion) attached near the central portion of the first sheet-shaped member 101 and a second sheet-shaped member 103. A second swelling suppressing portion (second suppressing portion) attached to the vicinity of the central portion of the swelling suppressing portion 109b constitutes the swelling suppressing portion 109b.

As shown in FIGS. 3A and 3B, the first and second swelling suppressing portions constituting the swelling suppressing portion 109b allow a pipette (not shown) to be inserted from the port portion 105. They are arranged so as to be parallel to each other and substantially linearly continuous while maintaining a predetermined distance.

Here, the first and second swelling suppressing portions constituting the swelling suppressing portion 109b are reinforcing members such as hard plastics. As a result, it is possible to suppress the shape of the storage chamber 113 capable of containing the storage liquid (liquid) 130 containing the sample derived from the living body collected from the living tissue so as not to be deformed more than necessary at the time of freezing and thawing. It becomes.

Other configurations are almost the same as the cell storage bag shown in the first embodiment.

According to the present embodiment, for example, as shown in FIGS. 3A and 3B, the first and second swelling constituting the swelling suppressing portion 109b are formed during use, particularly during freezing and thawing. The restraining portion makes it possible to easily suppress the large expansion of the storage chamber 113. Moreover, the first and second swelling suppressing portions constituting the swelling suppressing portion 109b do not prevent the storage liquid 130 from being taken out by the pipette 120 at the time of thawing.

The first and second swelling suppressing portions constituting the swelling suppressing portion 109b are attached not only in the direction along the insertion direction of the pipette 120 but also in the direction intersecting the insertion direction or in the diagonal direction of the storage chamber 113. You may be able to do it.

Further, the case is not limited to the case where it is attached to either one of the first sheet-like member 101 and the second sheet-like member 103, and it may be attached to both. That is, it is also possible to have a configuration in which the first sheet-shaped member 101 and the second sheet-shaped member 103 are adhered to each other via the first and second swelling suppressing portions constituting the swelling suppressing portion 109b. ..

Further, the first and second swelling suppressing portions constituting the swelling suppressing portion 109b may be arranged to face each other at substantially the same position of either one or both.

In either case, by increasing the number of swelling suppressing portions, it is possible to make the cross section of the accommodating chamber 113 thinner during use.

<Third Embodiment>
4 (a) and 4 (b) schematically show a configuration example of the cell storage bag (before use) according to the third embodiment of the present invention, and FIG. 4 (a) shows the upper surface. FIG. 4B is a front view. In addition, in FIG. 4A and FIG. 4B, the same or similar reference numerals are given to the same parts as the cell storage bag of the first embodiment, and detailed description thereof will be omitted.

That is, in the cell storage bag of the present embodiment, for example, as shown in FIGS. 4 (a) and 4 (b), the shape of the storage chamber 113 is not unnecessarily deformed during use, particularly during freezing and thawing. Is provided with one swelling suppressing portion 109a for reinforcing the container body 100. In the swelling suppressing portion 109a, for example, the first sheet-shaped member 101 and the second sheet-shaped member 103 are welded so as to be substantially linearly continuous in the vicinity of the central portion of the storage chamber 113.

Further, the container body 100 is welded between one edge portion of the first sheet-shaped member 101 and one edge portion of the second sheet-shaped member 103 so as to communicate with at least a part of the storage chamber 113. A port portion 115 is provided. The port portion 115 is for temporarily storing the storage liquid 130 containing a sample derived from a living body in the inside (reservoir portion) 115a, and is provided with, for example, a screw-type cap 107 that can be opened and closed with one hand. There is.

In the present embodiment, by inserting a pipette (not shown) into the port portion 115, the storage liquid 130 containing a sample derived from a living body can be taken in and out. Therefore, since the pipette is not inserted into the accommodating chamber 113, the first sheet-shaped member 101 and the second sheet-shaped member 103 are welded in the vicinity of the central portion of the accommodating chamber 113 so as to be substantially linearly continuous. It is possible to provide one swelling suppressing portion 109a.

Other configurations are almost the same as the cell storage bag shown in the first embodiment.

According to the present embodiment, for example, as shown in FIGS. 4 (a) and 4 (b), a storage chamber 113 is required by one swelling suppressing portion 109a during use, especially during freezing and thawing. It is possible to suppress the swelling as described above. In particular, the effect of suppressing the deformation of the accommodation chamber 113 due to the port portion 115 can be expected. Moreover, one swelling suppressing portion 109a does not hinder the removal of the storage liquid 130 by the pipette 120 at the time of thawing.

In the cell storage bag according to the third embodiment, one swelling suppressing portion may be dispersedly arranged in a plurality of broken lines, or a plurality of swelling suppressing portions may be arranged regardless of the insertion direction of the pipette. It can also be configured to be arranged.

Further, instead of the swelling suppressing portion 109a, the swelling suppressing portion 109b shown as the second embodiment can be applied.

<Fourth Embodiment>
FIG. 5 is a front view schematically showing a configuration example of a cell storage bag (before use) according to a fourth embodiment of the present invention. In FIG. 5, the same parts as the cell storage bag of the first embodiment are designated by the same or similar reference numerals, and detailed description thereof will be omitted.

That is, the cell storage bag of the present embodiment reinforces the container body 100 so that the shape of the storage chamber 113 is not unnecessarily deformed during use, particularly during freezing and thawing, as shown in FIG. 5, for example. The swelling suppressing portion 109c is provided. In the swelling suppressing portion 109c, the first sheet-shaped member and the second sheet-shaped member are substantially linearly continuous in the vicinity of the central portion of the accommodating chamber 113 along the insertion direction of the pipette (not shown). It is crimped.

In the present embodiment, the swelling suppressing portion 109c has a removable structure, for example, a first sheet-like member and a second sheet-like structure, in order to avoid hindering the insertion of the pipette into the storage chamber 113. The structure is such that the members are joined by crimping, which is weaker than welding.

For example, the swelling suppressing portion 109c, which is in a crimped state at the time of freezing and suppresses the swelling of the storage chamber 113, can be in a peeled state with the insertion of the pipette.

Other configurations are almost the same as the cell storage bag shown in the first embodiment.

According to the present embodiment, for example, as shown in FIG. 5, during use, especially during freezing and thawing, the swelling suppressing portion 109c can prevent the storage chamber 113 from swelling more than necessary. .. Moreover, it is inexpensive and the swelling suppressing portion 109c is configured to be peelable, so that it does not hinder the removal of the preservative solution by the pipette at the time of thawing.

The swelling suppressing portion 109c may be, for example, a linear hook-and-loop fastener or a chuck type or magnet type zipper that can be repeatedly attached and detached.

Further, the swelling suppressing portion 109c is not limited to one, and a configuration including a plurality of swelling suppressing portions is also possible (not shown).

<Fifth Embodiment>
FIG. 6 is a front view schematically showing a configuration example of a cell storage bag (before use) according to a fifth embodiment of the present invention. In FIG. 6, the same parts as the cell storage bag of the fourth embodiment are designated by the same or similar reference numerals, and detailed description thereof will be omitted.

That is, as shown in FIG. 6, for example, the cell storage bag of the present embodiment has a swelling suppressing portion 109d for reinforcing the shape of the storage chamber 113 so as not to be deformed more than necessary during use, particularly during freezing and thawing. However, they are distributed and arranged in a plurality of broken lines.

Other configurations are substantially the same as the cell storage bag shown in the fourth embodiment.

In the present embodiment, by arranging the swelling suppressing portions 109d in a dispersed manner, it is possible to easily suppress the swelling of the accommodation chamber 113, but it is easier to insert the pipette (not shown) into the accommodation chamber 113. It is possible to avoid being in a peeled state and hindering the removal of the storage liquid.

<Sixth Embodiment>
7 (a) and 7 (b) schematically show a configuration example of the cell storage bag (before use) according to the sixth embodiment of the present invention, and FIG. 7 (a) shows the front surface. FIG. 7 (b) is a cross-sectional view taken along the line 7b-7b of FIG. 7 (a). 8 (a) to 8 (c) schematically show an example of using the cell storage bag shown in FIGS. 7 (a) and 7 (b), and FIG. 8 (a) shows. 8 (b) is a front view, FIG. 8 (b) is a cross-sectional view taken along the line 8b-8b of FIG. 8 (a), and FIG. 8 (c) is a cross-sectional view taken along the line 8c-8c of FIG. 8 (a). In FIGS. 7 (a), 7 (b) and 8 (a) to 8 (c), the same parts as the cell storage bag of the fifth embodiment are designated by the same or similar reference numerals. , Detailed explanation is omitted.

In the case of the present embodiment, for example, as shown in FIGS. 7 (a) and 7 (b), a plurality of (here, four) swelling suppressing portions 109e are provided along the insertion direction of the pipette, which will be described later. It is configured to be divided into a plurality of parts in parallel with each other and in a substantially broken line shape while maintaining a predetermined distance.

Each of the four swelling suppressing portions 109e has a configuration in which the first sheet-shaped member 101 and the second sheet-shaped member 103 are crimped, for example, in the vicinity of substantially the central portion of the accommodation chamber 113.

Further, the four swelling suppressing portions 109e are provided at a predetermined distance, for example, at a distance narrower than the distance through which the pipette 120 can be inserted from the port portion 105, as shown in FIGS. 8 (a) to 8 (c). Have been placed.

In the present embodiment, since the distance between the swelling suppressing portions 109e is narrower than the thickness (diameter) of the pipette 120, the swelling suppressing portion 109e is easily peeled off as the pipette 120 is inserted into the storage chamber 113. Become. As a result, the swelling suppressing portion 109e can easily prevent the storage liquid 130 from being hindered from being taken out while suppressing the swelling of the storage chamber 113.

That is, as the pipette 120 is inserted into the accommodation chamber 113 from the port portion 105, the swelling suppressing portion 109e is formed by crimping to the extent that the pipette 120 is in a peeled state, so that the swelling suppressing portion 109e swells at intervals narrower than the thickness of the pipette 120. The suppression unit 109e can be arranged. Therefore, the storage chamber 113 whose cross-sectional shape is easily deformed can be reinforced by more swelling suppressing portions 109e. Moreover, the storage liquid 130 can be taken in and out by the existing pipette 120 without newly developing a dedicated pipette according to the interval of the swelling suppressing portion 109e, and in particular, it can be easily taken out.

The storage liquid 130 containing the sample derived from a living body is stored so as not to peel off the swelling suppressing portion 109e from a location close to the port portion 105.

According to the present embodiment, for example, as shown in FIGS. 8A to 8C, a storage chamber 113 is required by the four swelling suppressing portions 109e during use, especially during freezing and thawing. It is possible to suppress the swelling as described above. Moreover, the four swelling suppressing portions 109a do not prevent the pipette 120 from taking out the storage solution 130 at the time of thawing, and the tip portion of the pipette 120 can easily reach the vicinity of the deepest portion of the storage chamber 113. NS. Therefore, an inexpensive cell storage bag can be obtained in which the storage solution 130 can be taken in and out by the existing pipette 120.

The swelling suppressing portion 109e is not limited to four, and the swelling suppressing portion may be arranged so as to be substantially linearly continuous.

<Other embodiments>
In each of the cell storage bags of the above-described embodiments, the storage chamber 113 formed by the first sheet-shaped member 101 and the second sheet-shaped member 103 is configured to have a more sufficient capacity. It is also possible to do.

That is, for example, as shown in FIG. 9A, the swelling suppressing portion 109 is formed with a margin in the first sheet-shaped member 101 so that the accommodation chamber 113 has a sufficient capacity. You may.

Here, the swelling suppressing portion 109 is a general term for the above-mentioned swelling suppressing portions 109a, 109b, 109c, 109d, 109e.

For example, as shown in FIG. 9B, the swelling suppressing portion 109 is formed with a margin in the second sheet-shaped member 103 so that the accommodation chamber 113 has a sufficient capacity. You may.

Alternatively, as shown in FIG. 9C, for example, the first sheet-shaped member 101 and the second sheet-shaped member 103 are swollen with a margin so that the storage chamber 113 has a sufficient capacity. The restraining portion 109 may be formed.

As described above, according to the cell storage bag according to each embodiment of the present invention, in any case, it is possible to prevent the storage chamber 113 of the cell storage bag from swelling more than necessary during freezing and thawing. Will be.

That is, the swelling suppressing portion 109 (109a, 109b) is placed in the storage chamber 113 of the container body 100 including the first sheet-shaped member 101 and the second sheet-shaped member 103, which houses the storage liquid 130 containing the sample derived from the living body. , 109c, 109d, 109e). As a result, even when the sheet thickness of the first sheet-shaped member 101 and the second sheet-shaped member 103 is made thinner, the storage chamber 113 can be prevented from expanding and deforming during freezing and thawing. become. As a result, the sheet thickness of the first sheet-shaped member 101 and the second sheet-shaped member 103 can be made as thin as possible. Therefore, heat transfer to the storage solution 130 can be improved without damaging the cells or breaking the cell storage bag, and the freezing time and thawing time of the storage solution 130 can be significantly shortened. It is a thing.

In each embodiment, the cell storage bag includes the ports 105 and 115 with screw-type caps 107, but the present invention is not limited to this, and the cells are sealed with a seal or the like. It may be.

Although one aspect of the present invention has been described above by exemplifying the embodiments, it is an example, and the scope of the invention described in the claims can be variously changed within a range that does not deviate from the gist of the invention. be.

100 Container body (bag body)
101 First sheet-like member 103 Second sheet-like member 105, 115 Port portion 107 Screw-type cap (cover portion)
109, 109a, 109b, 109c, 109d, 109e Swelling suppression unit (expansion suppression unit)
111 Peripheral welding part 113 Containment chamber 120 Pipette 130 Preservative solution (liquid containing biological sample)

In order to achieve the above object, in the cryopreservation container of one aspect of the present invention, the peripheral edge portion of the first sheet-shaped member and the peripheral edge portion of the second sheet-shaped member are welded to a portion other than the peripheral edge portion. A cryopreservation container having a bag body in which a storage chamber containing a liquid containing a living body-derived sample is formed, and a pipette can be inserted into the bag body. The bag body contains the liquid. It is characterized in that an expansion suppressing portion that suppresses excessive deformation of the shape of the storage chamber during freezing and thawing is provided so as to be separable with the insertion of the pipette.
Further, in the cryopreservation container of another aspect of the present invention, the peripheral edge portion of the first sheet-shaped member and the peripheral edge portion of the second sheet-shaped member are welded, and the peripheral portion excluding the peripheral edge portion is derived from a living body. A cryopreservation container provided with a bag body in which a storage chamber capable of containing a liquid containing a sample is formed, and the shape of the storage chamber is excessively deformed in the bag body when the liquid is frozen and thawed. The peripheral portion of the first sheet-shaped member and the peripheral edge portion of the second sheet-shaped member are provided with an expansion suppressing portion that suppresses the expansion and communicate with at least a part of the storage chamber. A port portion having a lid portion that can be opened and closed while being welded is provided in a direction intersecting the installation direction of the expansion suppressing portion.

Claims (9)

The peripheral edge of the first sheet-shaped member and the peripheral edge of the second sheet-shaped member were welded together, and a storage chamber capable of containing a liquid containing a biological sample was formed in a portion other than the peripheral edge. A cryopreservation container with a bag
A cryopreservation container characterized in that the bag body is provided with an expansion suppressing portion that suppresses excessive deformation of the shape of the storage chamber when the liquid is frozen and thawed. The cryopreservation container according to claim 1, wherein the expansion suppressing portion is continuously arranged linearly in a portion of the storage chamber. The cryopreservation container according to claim 1, wherein the expansion suppressing portions are dispersedly arranged in a broken line shape in the storage chamber portion. The expansion suppressing portion is characterized in that the first sheet-shaped member and the second sheet-shaped member are crimped to each other and can be separated as the pipette is inserted. The cryopreservation container according to any one of claims 1 to 3. The cryopreservation container according to claim 1 or 2, wherein a plurality of the expansion suppressing portions are arranged at a distance such that a pipette can be inserted. The expansion suppressing portion according to claim 1, 2 or 5, wherein the vicinity of the central portion of the first sheet-shaped member and the vicinity of the central portion of the second sheet-shaped member are welded together. Cryopreservation container. The expansion suppressing portion includes a first suppressing portion for reinforcement attached near the central portion of the first sheet-shaped member and a second reinforcing portion attached near the central portion of the second sheet-shaped member. The cryopreservation container according to claim 1, 2 or 5, wherein the holding portion and the holding portion are arranged to face each other. A port having a lid that can be opened and closed while being welded to the peripheral edge of the first sheet-shaped member and the peripheral edge of the second sheet-shaped member so as to communicate with at least a part of the storage chamber. The cryopreservation container according to any one of claims 1 to 7, further comprising a part. The cryopreservation container according to claim 8, wherein the lid is formed of a screw-type cap.

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