JP7082377B2 - Freezing storage container - Google Patents

Freezing storage container Download PDF

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JP7082377B2
JP7082377B2 JP2020017810A JP2020017810A JP7082377B2 JP 7082377 B2 JP7082377 B2 JP 7082377B2 JP 2020017810 A JP2020017810 A JP 2020017810A JP 2020017810 A JP2020017810 A JP 2020017810A JP 7082377 B2 JP7082377 B2 JP 7082377B2
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sheet
shaped member
pipette
bag
peripheral edge
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JP2021122493A (en
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綾子 松澤
八州 百瀬
泰彦 田畑
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Yamato Scientific Co Ltd
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    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61JCONTAINERS SPECIALLY ADAPTED FOR MEDICAL OR PHARMACEUTICAL PURPOSES; DEVICES OR METHODS SPECIALLY ADAPTED FOR BRINGING PHARMACEUTICAL PRODUCTS INTO PARTICULAR PHYSICAL OR ADMINISTERING FORMS; DEVICES FOR ADMINISTERING FOOD OR MEDICINES ORALLY; BABY COMFORTERS; DEVICES FOR RECEIVING SPITTLE
    • A61J1/00Containers specially adapted for medical or pharmaceutical purposes
    • A61J1/05Containers specially adapted for medical or pharmaceutical purposes for collecting, storing or administering blood, plasma or medical fluids ; Infusion or perfusion containers
    • A61J1/10Bag-type containers
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61JCONTAINERS SPECIALLY ADAPTED FOR MEDICAL OR PHARMACEUTICAL PURPOSES; DEVICES OR METHODS SPECIALLY ADAPTED FOR BRINGING PHARMACEUTICAL PRODUCTS INTO PARTICULAR PHYSICAL OR ADMINISTERING FORMS; DEVICES FOR ADMINISTERING FOOD OR MEDICINES ORALLY; BABY COMFORTERS; DEVICES FOR RECEIVING SPITTLE
    • A61J3/00Devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B65CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
    • B65DCONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
    • B65D77/00Packages formed by enclosing articles or materials in preformed containers, e.g. boxes, cartons, sacks or bags

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  • General Health & Medical Sciences (AREA)
  • Veterinary Medicine (AREA)
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  • Engineering & Computer Science (AREA)
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  • Medical Preparation Storing Or Oral Administration Devices (AREA)
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  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Description

本発明は、細胞などの生体組織の凍結保存に用いられる細胞保存用バッグとして適用される凍結保存容器に関する。 The present invention relates to a cryopreservation container applied as a cell preservation bag used for cryopreservation of living tissues such as cells.

従来、凍結保存容器は、再生医療や遺伝子治療、移植治療などの分野において、様々な用途に広く利用されている。即ち、生体組織より採取された細胞は、採取されてから再生医療などに実際に用いられるまでの間、凍結保存容器内に収容された状態で凍結され、凍結保存容器ごと保存される(例えば、特許文献1参照)。 Conventionally, cryopreservation containers have been widely used for various purposes in fields such as regenerative medicine, gene therapy, and transplantation therapy. That is, cells collected from living tissue are frozen in a state of being contained in a cryopreservation container from the time they are collected until they are actually used for regenerative medicine, and are stored together with the cryopreservation container (for example,). See Patent Document 1).

細胞の凍結保存に用いられる一般的な細胞保存用バッグの場合、凍結時には、細胞を懸濁させた保存液を内部に収容した状態で、液体窒素を用いて急速に凍結されるとともに、解凍時には、ぬるめの温水を用いて徐々に解凍されるようになっている。 In the case of a general cell storage bag used for cryopreservation of cells, when frozen, the cells are rapidly frozen using liquid nitrogen with the storage solution containing the suspended cells inside, and at the time of thawing. , It is gradually thawed using lukewarm warm water.

特開2015-42212号公報Japanese Unexamined Patent Publication No. 2015-42212

ところで、上記のような再生医療などの分野においては、細胞の要素構造と生物機能を壊さない程度に、保存液の凍結時間や解凍時間をできるだけ短くしたいという要求がある。 By the way, in the above-mentioned fields such as regenerative medicine, there is a demand to shorten the freezing time and thawing time of the preservation solution as much as possible so as not to destroy the elemental structure and biological function of cells.

細胞の要素構造と生物機能を壊すことなく、保存液の凍結時間や解凍時間を短くするためには、熱の伝わりが良くなるように、細胞保存用バッグを構成するシート状部材のシート厚をできるだけ薄くすればよい。シート状部材は、薄くなればなるほど熱を伝え易くなるため、超低温状態での使用にも耐え得る程度に、できるだけ薄くすることが望ましい。 In order to shorten the freezing time and thawing time of the storage solution without destroying the elemental structure and biological functions of the cells, the sheet thickness of the sheet-like members constituting the cell storage bag should be increased so that heat transfer is improved. Make it as thin as possible. The thinner the sheet-shaped member, the easier it is to transfer heat, so it is desirable to make the sheet-shaped member as thin as possible so that it can withstand use in ultra-low temperature conditions.

しかしながら、シート厚を薄くし過ぎると、保存液の体積の変化などにより細胞保存用バッグが膨らむなど、変形し易くなる。細胞保存用バッグの変形は、収容されている保存液の温度分布や濃度分布を不均一にする要因となり、凍結や解凍の均一性を損なう結果となる。このため、シート厚を薄くするだけでは、簡単には保存液の凍結時間や解凍時間を短くできないという課題があった。 However, if the sheet thickness is made too thin, the cell storage bag may be inflated due to a change in the volume of the storage solution, and the cell storage bag may be easily deformed. Deformation of the cell storage bag causes the temperature distribution and concentration distribution of the stored storage liquid to become non-uniform, resulting in impairing the uniformity of freezing and thawing. Therefore, there is a problem that the freezing time and the thawing time of the preservation solution cannot be easily shortened only by reducing the sheet thickness.

本発明は、上記に鑑みてなされたもので、その目的とするところは、シート厚を薄くした場合にも、生体由来のサンプルを含む液体を収容する収容室が必要以上に膨らむのを防止でき、該液体の凍結時間や解凍時間を短くすることが可能な凍結保存容器を提供することにある。 The present invention has been made in view of the above, and an object of the present invention is to prevent the storage chamber containing a liquid containing a biological sample from swelling more than necessary even when the sheet thickness is reduced. The present invention is to provide a cryopreservation container capable of shortening the freezing time and thawing time of the liquid.

上記課題を達成するため、本発明の一態様の凍結保存容器は、第1のシート状部材の周縁部と第2のシート状部材の周縁部とが溶着されて、該周縁部を除く部分に、生体由来のサンプルを含む液体を収容可能な収容室が形成された袋体を備え、前記袋体内へのピペットの挿通が可能な凍結保存容器であって、前記袋体の内面には、前記第1のシート状部材と前記第2のシート状部材との間が前記溶着よりも脆弱に圧着されてなり、前記液体の凍結時および解凍時に、前記収容室の形状が過度に変形するのを抑制する膨張抑制部が、前記ピペットの挿通に伴って分離可能に設けられていることを特徴とする。
また、本発明の他の態様の凍結保存容器は、第1のシート状部材の周縁部と第2のシート状部材の周縁部とが溶着されて、該周縁部を除く部分に、生体由来のサンプルを含む液体を収容可能な収容室が形成された袋体を備え、前記袋体内へのピペットの挿通が可能な凍結保存容器であって、前記袋体の内面には、前記第1のシート状部材と前記第2のシート状部材との間が前記溶着よりも脆弱に圧着されてなり、前記液体の凍結時および解凍時に、前記収容室の形状が過度に変形するのを抑制する膨張抑制部が、前記ピペットの挿通位置に対応する前記収容室の中央部分に線状に連続配置されるとともに、前記ピペットの挿通に伴って分離可能に設けられていることを特徴とする。
また、本発明の他の態様の凍結保存容器は、第1のシート状部材の周縁部と第2のシート状部材の周縁部とが溶着されて、該周縁部を除く部分に、生体由来のサンプルを含む液体を収容可能な収容室が形成された袋体を備え、前記袋体内へのピペットの挿通が可能な凍結保存容器であって、前記袋体の内面には、前記第1のシート状部材と前記第2のシート状部材との間が前記溶着よりも脆弱に圧着されてなり、前記液体の凍結時および解凍時に、前記収容室の形状が過度に変形するのを抑制する膨張抑制部が、前記ピペットの挿通位置に対応する前記収容室の中央部分に破線状に分散配置されるとともに、前記ピペットの挿通に伴って分離可能に設けられていることを特徴とする。
また、本発明の他の態様の凍結保存容器は、第1のシート状部材の周縁部と第2のシート状部材の周縁部とが溶着されて、該周縁部を除く部分に、生体由来のサンプルを含む液体を収容可能な収容室が形成された袋体を備え、前記袋体内へのピペットの挿通が可能な凍結保存容器であって、前記袋体の内面には、前記第1のシート状部材と前記第2のシート状部材との間が前記溶着よりも脆弱に圧着されてなり、前記液体の凍結時および解凍時に、前記収容室の形状が過度に変形するのを抑制する膨張抑制部が、前記ピペットの挿通が部分的に可能な距離だけ離間して複数配置されるとともに、前記ピペットの挿通に伴って分離可能に設けられていることを特徴とする。
In order to achieve the above object, in the cryopreservation container of one aspect of the present invention, the peripheral edge portion of the first sheet-shaped member and the peripheral edge portion of the second sheet-shaped member are welded to a portion other than the peripheral edge portion. A cryopreservation container having a bag body in which a storage chamber containing a liquid containing a living body-derived sample is formed, and a pipette can be inserted into the bag body . The inner surface of the bag body has the above- mentioned The first sheet-shaped member and the second sheet-shaped member are crimped more fragilely than the welding, and the shape of the storage chamber is excessively deformed when the liquid is frozen and thawed. The expansion suppressing portion for suppressing is provided so as to be separable with the insertion of the pipette.
Further, in the cryopreservation container of another aspect of the present invention, the peripheral edge portion of the first sheet-shaped member and the peripheral edge portion of the second sheet-shaped member are welded, and the peripheral portion excluding the peripheral edge portion is derived from a living body. It is a cryopreservation container having a bag body in which a storage chamber containing a liquid containing a sample is formed, and a pipette can be inserted into the bag body. The inner surface of the bag body has the first sheet. The space between the shaped member and the second sheet-shaped member is crimped more fragilely than the welding, and expansion suppression that suppresses excessive deformation of the shape of the storage chamber during freezing and thawing of the liquid is suppressed. The portions are linearly and continuously arranged in the central portion of the storage chamber corresponding to the insertion position of the pipette, and are provided so as to be separable with the insertion of the pipette.
Further, in the cryopreservation container of another aspect of the present invention, the peripheral edge portion of the first sheet-shaped member and the peripheral edge portion of the second sheet-shaped member are welded, and the peripheral portion excluding the peripheral edge portion is derived from a living body. It is a cryopreservation container having a bag body in which a storage chamber containing a liquid containing a sample is formed, and a pipette can be inserted into the bag body. The inner surface of the bag body has the first sheet. The space between the shaped member and the second sheet-shaped member is crimped more fragilely than the welding, and expansion suppression that suppresses excessive deformation of the shape of the storage chamber during freezing and thawing of the liquid is suppressed. The portions are distributed and arranged in a broken line shape in the central portion of the storage chamber corresponding to the insertion position of the pipette, and are provided so as to be separable with the insertion of the pipette.
Further, in the cryopreservation container of another aspect of the present invention, the peripheral edge portion of the first sheet-shaped member and the peripheral edge portion of the second sheet-shaped member are welded, and the peripheral portion excluding the peripheral edge portion is derived from a living body. It is a cryopreservation container having a bag body in which a storage chamber containing a liquid containing a sample is formed, and a pipette can be inserted into the bag body. The inner surface of the bag body has the first sheet. The space between the shaped member and the second sheet-shaped member is crimped more fragilely than the welding, and expansion suppression that suppresses excessive deformation of the shape of the storage chamber during freezing and thawing of the liquid is suppressed. It is characterized in that a plurality of portions are arranged so as to be partially separated by a distance that allows the pipette to be inserted, and are provided so as to be separable with the insertion of the pipette.

本発明の一態様の凍結保存容器によれば、膨張抑制部を設けたことによって、第1のシート状部材および第2のシート状部材の厚さを薄くした場合にも、凍結時や解凍時に、収容室が変形し過ぎるのを抑制できるようになる。これにより、第1のシート状部材および第2のシート状部材の厚さをできる限り薄くすることが可能となる。したがって、生体由来のサンプルを含む液体の凍結時間や解凍時間をより短時間化できるようになる。 According to the cryopreservation container of one aspect of the present invention, even when the thickness of the first sheet-shaped member and the second sheet-shaped member is reduced by providing the expansion suppressing portion, at the time of freezing or thawing. , It becomes possible to prevent the containment chamber from being deformed too much. This makes it possible to reduce the thickness of the first sheet-shaped member and the second sheet-shaped member as much as possible. Therefore, the freezing time and thawing time of the liquid containing the sample derived from the living body can be shortened.

本発明によれば、シート厚を薄くした場合にも、生体由来のサンプルを含む液体を収容する収容室が必要以上に膨らむのを防止でき、該液体の凍結時間や解凍時間を短くすることが可能な凍結保存容器を提供できる。 According to the present invention, even when the sheet thickness is reduced, it is possible to prevent the storage chamber containing the liquid containing the biological sample from swelling more than necessary, and it is possible to shorten the freezing time and thawing time of the liquid. A possible cryopreservation container can be provided.

(a)~(c)は、第1実施形態に係る凍結保存容器が適用される細胞保存用バッグの構成例を示す概略図である。(A) to (c) are schematic views showing a configuration example of a cell storage bag to which the cryopreservation container according to the first embodiment is applied. (a)~(c)は、図1に示す細胞保存用バッグの使用例を示す概略図である。(A) to (c) are schematic views showing an example of use of the cell storage bag shown in FIG. 1. (a),(b)は、第2実施形態に係る細胞保存用バッグの構成例を示す概略図である。(A) and (b) are schematic views showing the configuration example of the cell storage bag according to the second embodiment. (a),(b)は、第3実施形態に係る細胞保存用バッグの構成例を示す概略図である。(A) and (b) are schematic views showing a configuration example of a cell storage bag according to a third embodiment. 第4実施形態に係る細胞保存用バッグの構成例を示す概略平面図である。It is a schematic plan view which shows the structural example of the cell storage bag which concerns on 4th Embodiment. 第5実施形態に係る細胞保存用バッグの構成例を示す概略平面図である。It is a schematic plan view which shows the structural example of the cell storage bag which concerns on 5th Embodiment. (a),(b)は、第6実施形態に係る細胞保存用バッグの構成例を示す概略図である。(A) and (b) are schematic views showing the configuration example of the cell storage bag according to the sixth embodiment. (a)~(c)は、図7に示す細胞保存用バッグの使用例を示す概略図である。(A) to (c) are schematic views showing an example of use of the cell storage bag shown in FIG. 7. (a)~(c)は、その他の実施形態に係る細胞保存用バッグの構成例を示す概略断面図である。(A) to (c) are schematic cross-sectional views showing a configuration example of a cell storage bag according to another embodiment.

以下、図面を参照して、本発明に係る実施の形態について説明する。なお、各実施の形態において、図面は、細胞保存用バッグの構成を模式的に示すものであって、実際のものとは異なるものであることに留意すべきである。 Hereinafter, embodiments according to the present invention will be described with reference to the drawings. It should be noted that in each embodiment, the drawings schematically show the configuration of the cell storage bag and are different from the actual ones.

<第1実施形態>
図1(a)~図1(c)は、本発明の第1実施形態に係る凍結保存容器が適用される細胞保存用バッグの構成例を概略的に示すものであって、図1(a)は上面図、図1(b)は正面図(平面図)、図1(c)は図1(b)の1c-1c線に沿う断面図である。また、図2(a)~図2(c)は、図1(a)~図1(c)に示す細胞保存用バッグの使用例を概略的に示すものであって、図2(a)は正面図、図2(b)は図2(a)の2b-2b線に沿う断面図、図2(c)は図2(a)の2c-2c線に沿う断面図である。
<First Embodiment>
1 (a) to 1 (c) schematically show a configuration example of a cell storage bag to which the cryopreservation container according to the first embodiment of the present invention is applied, and FIGS. 1 (a) to 1 (a). ) Is a top view, FIG. 1 (b) is a front view (plan view), and FIG. 1 (c) is a cross-sectional view taken along line 1c-1c of FIG. 1 (b). 2 (a) to 2 (c) schematically show an example of using the cell storage bag shown in FIGS. 1 (a) and 1 (c), and FIG. 2 (a) shows. 2 (b) is a front view, FIG. 2 (b) is a cross-sectional view taken along line 2b-2b of FIG. 2 (a), and FIG. 2 (c) is a cross-sectional view taken along line 2c-2c of FIG. 2 (a).

図1(a)~図1(c)に示すように、本実施形態の細胞保存用バッグは、2枚の樹脂製のシート状部材101、103の周縁部分が相互に溶着されてなる構成の、容器本体(袋体)100を備えている。 As shown in FIGS. 1A to 1C, the cell storage bag of the present embodiment has a structure in which peripheral portions of two resin sheet-shaped members 101 and 103 are welded to each other. , The container body (bag body) 100 is provided.

より詳しくは、容器本体100は、樹脂製の第1のシート状部材101の周縁部と樹脂製の第2のシート状部材103の周縁部とが溶着された周縁溶着部111を備えている。そして、周縁溶着部111によって囲まれた部分(空間領域)に、例えば図2(a)~図2(c)に示すように、生体由来のサンプルを含む保存液(液体)130が収容される収容室113が形成されている。生体由来のサンプルの具体例としては、細胞、細胞の3D凝集体、細胞シートやその積層体、生体組織およびその破砕物、細胞内オルガネラ、細胞外分泌物(エクソソームなど)、2種類以上の細胞からなる3D凝集体(オルガノイドなど)、細胞シートおよびその積層体である。 More specifically, the container body 100 includes a peripheral welding portion 111 in which the peripheral edge portion of the first resin sheet-shaped member 101 and the peripheral edge portion of the second resin sheet-shaped member 103 are welded. Then, as shown in FIGS. 2 (a) to 2 (c), for example, a storage liquid (liquid) 130 containing a sample derived from a living body is housed in a portion (spatial region) surrounded by the peripheral welded portion 111. A containment chamber 113 is formed. Specific examples of biological samples include cells, 3D aggregates of cells, cell sheets and their laminates, biological tissues and their disruptions, intracellular organellas, extracellular secretions (exosomes, etc.) from two or more types of cells. 3D aggregates (organoids, etc.), cell sheets and laminates thereof.

容器本体100には、後述するピペット(スポイト)の挿通方向に沿って、凍結時および解凍時に、収容室113の形状が必要以上に大きく変形するのを抑制するための膨れ抑制部(膨張抑制部)109aが設けられている。即ち、複数本(ここでは、2本)の膨れ抑制部109aを設けることによって、容器本体100を構成する第1のシート状部材101および第2のシート状部材103が膨らみ難くなるように補強されている。 The container body 100 has a swelling suppressing portion (expansion suppressing portion) for suppressing the shape of the storage chamber 113 from being deformed more than necessary during freezing and thawing along the insertion direction of the pipette (dropper) described later. ) 109a is provided. That is, by providing a plurality of (here, two) swelling suppressing portions 109a, the first sheet-shaped member 101 and the second sheet-shaped member 103 constituting the container body 100 are reinforced so as to be difficult to swell. ing.

また、容器本体100には、収容室113の少なくとも一部に連通するように、第1のシート状部材101の周縁部分と第2のシート状部材103の周縁部分との間に溶着されてなるポート部105が設けられている。ポート部105は、保存液130の出し入れを行うためのもので、例えば、片手での開閉が可能なスクリュー式のキャップ(蓋部)107を備えている。 Further, the container body 100 is welded between the peripheral edge portion of the first sheet-shaped member 101 and the peripheral edge portion of the second sheet-shaped member 103 so as to communicate with at least a part of the storage chamber 113. A port portion 105 is provided. The port portion 105 is for taking in and out the storage liquid 130, and is provided with, for example, a screw-type cap (cover portion) 107 that can be opened and closed with one hand.

本実施形態においては、例えば図2(a)に示すように、2本の膨れ抑制部109aが、ポート部105からのピペット120の挿通が可能な距離だけ離間して配置されている。即ち、2本の膨れ抑制部109aは、例えば図1(b),図1(c)に示すように、第1のシート状部材101と第2のシート状部材103とが収容室113の中央部付近の2か所において、それぞれ、所定の距離を保ちつつ、互いに平行に、かつ、ほぼ線状に連続するようにして溶着されている。 In the present embodiment, for example, as shown in FIG. 2A, the two swelling suppressing portions 109a are arranged apart from each other by a distance that allows the pipette 120 to be inserted from the port portion 105. That is, in the two swelling suppressing portions 109a, for example, as shown in FIGS. 1 (b) and 1 (c), the first sheet-shaped member 101 and the second sheet-shaped member 103 are in the center of the accommodating chamber 113. At two locations near the portion, each is welded so as to be parallel to each other and substantially linearly continuous while maintaining a predetermined distance.

これにより、例えば図2(a)~図2(c)に示すように、ピペット120は、2本の膨れ抑制部109aに沿って収容室113内に挿通可能とされるとともに、先端部分が収容室113の最深部113aの近傍にまで容易に到達可能とされている。 As a result, for example, as shown in FIGS. 2A to 2C, the pipette 120 can be inserted into the storage chamber 113 along the two swelling suppressing portions 109a, and the tip portion is accommodated. It is said that the vicinity of the deepest portion 113a of the chamber 113 can be easily reached.

ここで、第1のシート状部材101および第2のシート状部材103には、断熱能がないよう、例えば、0.3mm厚程度以下、好ましくは0.2mmよりも薄いEVA樹脂(エチレン-酢酸ビニル共重合樹脂)などの可撓性を有する熱可塑性樹脂が用いられる。第1のシート状部材101および第2のシート状部材103の膜厚(シート厚)としては、細胞の要素構造と生物機能を壊したりすることなく、超低温状態での使用にも耐え得る程度まで最薄化できる。 Here, the EVA resin (ethylene-acetic acid) having a thickness of, for example, about 0.3 mm or less, preferably thinner than 0.2 mm, so that the first sheet-shaped member 101 and the second sheet-shaped member 103 do not have heat insulating ability. A flexible thermoplastic resin such as vinyl copolymer resin) is used. The film thickness (sheet thickness) of the first sheet-shaped member 101 and the second sheet-shaped member 103 is such that it can withstand use in an ultra-low temperature state without damaging the element structure and biological function of cells. Can be diluted.

なお、EVA樹脂に限らず、例えば、環状オレフィン重合体(COC樹脂)などを用いることも可能である。 Not limited to EVA resin, for example, a cyclic olefin polymer (COC resin) or the like can also be used.

凍結時、細胞保存用バッグは、収容室113内に生体由来のサンプルを含む保存液130を収容した状態において、例えば、-196℃の液体窒素内に沈められる。これにより、生体由来のサンプルを含む保存液130は、細胞保存用バッグごと凍結保存される。 At the time of freezing, the cell storage bag is submerged in liquid nitrogen at, for example, -196 ° C. in a state where the storage solution 130 containing a sample derived from a living body is contained in the storage chamber 113. As a result, the preservation solution 130 containing the sample derived from the living body is cryopreserved together with the cell preservation bag.

この場合、細胞保存用バッグは、2本の膨れ抑制部109aによって収容室113が必要以上に膨張することなく、生体由来のサンプルを含む保存液130を、ほぼ一様の温度勾配および濃度勾配を有して凍結させることが可能となる。 In this case, the cell storage bag has a storage solution 130 containing a biological sample having a substantially uniform temperature gradient and concentration gradient without the storage chamber 113 being expanded more than necessary by the two swelling suppressing portions 109a. It can be held and frozen.

一方、解凍時には、細胞保存用バッグが液体窒素内より取り出され、例えば、37℃程度に温度制御されたぬるめの温水に細胞保存用バッグごと浸される。これにより、収容室113内の凍結状態にある保存液130を、ほぼ一様の温度勾配および濃度勾配を有して解凍させることが可能となる。 On the other hand, at the time of thawing, the cell storage bag is taken out from the liquid nitrogen, and for example, the cell storage bag is immersed in warm water whose temperature is controlled to about 37 ° C. This makes it possible to thaw the frozen storage liquid 130 in the storage chamber 113 with a substantially uniform temperature gradient and concentration gradient.

本実施形態によれば、例えば図2(a)~図2(c)に示すように、使用時、特に凍結時および解凍時においては、2本の膨れ抑制部109aによって、収容室113が必要以上に膨らむのを抑制することが可能となる。しかも、2本の膨れ抑制部109aが、解凍時にピペット120による保存液130の出し入れ(特に、取り出し)を妨げることもない。 According to the present embodiment, for example, as shown in FIGS. 2A to 2C, a storage chamber 113 is required by the two swelling suppressing portions 109a during use, especially during freezing and thawing. It is possible to suppress the swelling above. Moreover, the two swelling suppressing portions 109a do not prevent the storage liquid 130 from being taken in and out (particularly from being taken out) by the pipette 120 at the time of thawing.

即ち、本実施形態に係る細胞保存用バッグは、2本の膨れ抑制部109aによって、収容室113の断面を可能な限り薄く、しかも、ほぼ均一な厚さに保つことができるようになる。これにより、収容物である保存液130に対する凍結時および解凍時の熱の伝わりを、できるだけ均等にすることが可能となる。したがって、温度勾配や濃度勾配を招くことなく、保存液130の凍結時間や解凍時間をより短時間化できるようになる。 That is, in the cell storage bag according to the present embodiment, the two swelling suppressing portions 109a make it possible to keep the cross section of the storage chamber 113 as thin as possible and have a substantially uniform thickness. This makes it possible to make the heat transfer to the storage liquid 130, which is the contained material, during freezing and thawing as even as possible. Therefore, the freezing time and the thawing time of the storage liquid 130 can be shortened without causing a temperature gradient or a concentration gradient.

特に、膨れ抑制部109aを、第1のシート状部材101および第2のシート状部材103の一部を溶着させたことにより、使い捨てが可能な細胞保存用バッグを安価に提供できる。 In particular, by welding a part of the first sheet-shaped member 101 and the second sheet-shaped member 103 to the swelling suppressing portion 109a, a disposable cell storage bag can be provided at low cost.

なお、第1実施形態に係る細胞保存用バッグにおいては、破線状に複数に分散配置してなる膨れ抑制部としても良い(図示省略)。 In the cell storage bag according to the first embodiment, the swelling suppressing portion may be dispersed and arranged in a plurality of broken lines (not shown).

いずれの場合においても、膨れ抑制部の数を増やすことによって、使用時の収容室113の断面をより薄くなるようにすることが可能である。 In any case, by increasing the number of swelling suppressing portions, it is possible to make the cross section of the accommodating chamber 113 thinner during use.

<第2実施形態>
図3(a)および図3(b)は、本発明の第2実施形態に係る細胞保存用バッグ(使用前)の構成例を概略的に示すものであって、図3(a)は正面図、図3(b)は図3(a)の3b-3b線に沿う断面図である。なお、図3(a)および図3(b)において、第1実施形態の細胞保存用バッグと同一部分には、同一または類似の符号を付し、詳しい説明は省略する。
<Second Embodiment>
3 (a) and 3 (b) schematically show a configuration example of a cell storage bag (before use) according to a second embodiment of the present invention, and FIG. 3 (a) shows a front surface. FIG. 3 (b) is a cross-sectional view taken along the line 3b-3b of FIG. 3 (a). In FIGS. 3 (a) and 3 (b), the same or similar reference numerals are given to the same parts as the cell storage bag of the first embodiment, and detailed description thereof will be omitted.

即ち、本実施形態の細胞保存用バッグは、例えば、第1のシート状部材101の中央部付近に取り付けられた第1の膨れ抑制部(第1抑制部)と、第2のシート状部材103の中央部付近に取り付けられた第2の膨れ抑制部(第2抑制部)と、によって、膨れ抑制部109bが構成されている。 That is, the cell storage bag of the present embodiment has, for example, a first swelling suppressing portion (first suppressing portion) attached near the central portion of the first sheet-shaped member 101 and a second sheet-shaped member 103. The swelling suppressing portion 109b is configured by the second swelling suppressing portion (second suppressing portion) attached near the central portion of the above.

膨れ抑制部109bを構成する第1,第2の膨れ抑制部は、図3(a)および図3(b)に示すように、ポート部105からのピペット(図示省略)の挿通が可能なように、それぞれ、所定の距離を保ちつつ、互いに平行に、かつ、ほぼ線状に連続するようにして配置されている。 As shown in FIGS. 3A and 3B, the first and second swelling suppressing portions constituting the swelling suppressing portion 109b allow a pipette (not shown) to be inserted from the port portion 105. They are arranged so as to be parallel to each other and substantially linearly continuous while maintaining a predetermined distance.

ここで、膨れ抑制部109bを構成する第1,第2の膨れ抑制部は、例えば硬質プラスチックスなどの補強部材である。これにより、生体組織より採取された生体由来のサンプルを含む保存液(液体)130を収容可能な収容室113の形状が、凍結時および解凍時に、必要以上に変形しないように抑制することが可能となる。 Here, the first and second swelling suppressing portions constituting the swelling suppressing portion 109b are reinforcing members such as hard plastics. As a result, it is possible to suppress the shape of the storage chamber 113 that can store the storage liquid (liquid) 130 containing the sample derived from the living body collected from the living tissue so as not to be deformed more than necessary at the time of freezing and thawing. It becomes.

その他の構成は、第1実施形態で示した細胞保存用バッグとほぼ同様である。 Other configurations are almost the same as the cell storage bag shown in the first embodiment.

本実施形態によれば、例えば図3(a)および図3(b)に示すように、使用時、特に凍結時および解凍時においては、膨れ抑制部109bを構成する第1,第2の膨れ抑制部によって、収容室113が大きく膨らむのを容易に抑制することが可能となる。しかも、膨れ抑制部109bを構成する第1,第2の膨れ抑制部が、解凍時にピペット120による保存液130の取り出しを妨げることもない。 According to the present embodiment, for example, as shown in FIGS. 3A and 3B, the first and second swelling constituting the swelling suppressing portion 109b are formed during use, especially during freezing and thawing. The restraining portion makes it possible to easily suppress the large expansion of the storage chamber 113. Moreover, the first and second swelling suppressing portions constituting the swelling suppressing portion 109b do not prevent the storage liquid 130 from being taken out by the pipette 120 at the time of thawing.

なお、膨れ抑制部109bを構成する第1,第2の膨れ抑制部は、ピペット120の挿通方向に沿う方向に限らず、挿通方向と交差する方向、または、収容室113の対角方向に取り付けられるようにしても良い。 The first and second swelling suppressing portions constituting the swelling suppressing portion 109b are attached not only in the direction along the insertion direction of the pipette 120 but also in the direction intersecting the insertion direction or in the diagonal direction of the storage chamber 113. You may be able to do it.

また、第1のシート状部材101および第2のシート状部材103のいずれか一方に取り付けられる場合に限らず、両方に取り付けられるようにしても良い。つまり、第1のシート状部材101および第2のシート状部材103を、膨れ抑制部109bを構成する第1,第2の膨れ抑制部を介して相互に接着させる構成とすることも可能である。 Further, the case is not limited to the case where it is attached to either one of the first sheet-shaped member 101 and the second sheet-shaped member 103, and it may be attached to both. That is, it is also possible to have a configuration in which the first sheet-shaped member 101 and the second sheet-shaped member 103 are bonded to each other via the first and second swelling suppressing portions constituting the swelling suppressing portion 109b. ..

また、膨れ抑制部109bを構成する第1,第2の膨れ抑制部は、いずれか一方または両方の、ほぼ同じ位置において対向配置するようにしても良い。 Further, the first and second swelling suppressing portions constituting the swelling suppressing portion 109b may be arranged to face each other at substantially the same position of either one or both.

いずれの場合においても、膨れ抑制部の数を増やすことによって、使用時の収容室113の断面がより薄くなるようにすることが可能である。 In any case, by increasing the number of swelling suppressing portions, it is possible to make the cross section of the accommodation chamber 113 thinner during use.

<第3実施形態>
図4(a)および図4(b)は、本発明の第3実施形態に係る細胞保存用バッグ(使用前)の構成例を概略的に示すものであって、図4(a)は上面図、図4(b)は正面図である。なお、図4(a)および図4(b)において、第1実施形態の細胞保存用バッグと同一部分には、同一または類似の符号を付し、詳しい説明は省略する。
<Third Embodiment>
4 (a) and 4 (b) schematically show a configuration example of the cell storage bag (before use) according to the third embodiment of the present invention, and FIG. 4 (a) shows the upper surface. FIG. 4 (b) is a front view. In FIGS. 4 (a) and 4 (b), the same or similar reference numerals are given to the same parts as the cell storage bag of the first embodiment, and detailed description thereof will be omitted.

即ち、本実施形態の細胞保存用バッグは、例えば図4(a)および図4(b)に示すように、使用時、特に凍結時および解凍時に収容室113の形状が必要以上に変形しないように、容器本体100を補強するための1本の膨れ抑制部109aが設けられている。この膨れ抑制部109aは、例えば、第1のシート状部材101と第2のシート状部材103とが収容室113の中央部付近において、ほぼ線状に連続するようにして溶着されている。 That is, in the cell storage bag of the present embodiment, for example, as shown in FIGS. 4 (a) and 4 (b), the shape of the storage chamber 113 is not unnecessarily deformed during use, particularly during freezing and thawing. Is provided with one swelling suppressing portion 109a for reinforcing the container body 100. In the swelling suppressing portion 109a, for example, the first sheet-shaped member 101 and the second sheet-shaped member 103 are welded so as to be substantially linearly continuous in the vicinity of the central portion of the accommodation chamber 113.

また、容器本体100には、収容室113の少なくとも一部に連通するように、第1のシート状部材101の一縁部分と第2のシート状部材103の一縁部分との間に溶着されてなるポート部115が設けられている。このポート部115は、生体由来のサンプルを含む保存液130を内部(溜池部)115aに一時的に貯留するためのもので、例えば、片手での開閉が可能なスクリュー式のキャップ107を備えている。 Further, the container body 100 is welded between one edge portion of the first sheet-shaped member 101 and one edge portion of the second sheet-shaped member 103 so as to communicate with at least a part of the storage chamber 113. A port portion 115 is provided. The port portion 115 is for temporarily storing the storage liquid 130 containing a sample derived from a living body in the inside (reservoir portion) 115a, and is provided with, for example, a screw-type cap 107 that can be opened and closed with one hand. There is.

本実施形態においては、ポート部115内へのピペット(図示省略)の挿通により、生体由来のサンプルを含む保存液130の出し入れが可能とされる。そのため、収容室113内にピペットが挿通されないことから、収容室113の中央部付近に、第1のシート状部材101と第2のシート状部材103とがほぼ線状に連続するようにして溶着された1本の膨れ抑制部109aを設けることが可能である。 In the present embodiment, by inserting a pipette (not shown) into the port portion 115, the storage liquid 130 containing a sample derived from a living body can be taken in and out. Therefore, since the pipette is not inserted into the accommodating chamber 113, the first sheet-shaped member 101 and the second sheet-shaped member 103 are welded in the vicinity of the central portion of the accommodating chamber 113 so as to be substantially linearly continuous. It is possible to provide one swelling suppressing portion 109a.

その他の構成は、第1実施形態で示した細胞保存用バッグとほぼ同様である。 Other configurations are almost the same as the cell storage bag shown in the first embodiment.

本実施形態によれば、例えば図4(a)および図4(b)に示すように、使用時、特に凍結時および解凍時においては、1本の膨れ抑制部109aによって、収容室113が必要以上に膨らむのを抑制することが可能となる。特に、ポート部115による収容室113の変形を抑制する効果も期待できる。しかも、1本の膨れ抑制部109aが、解凍時にピペット120による保存液130の取り出しの妨げとなることもない。 According to the present embodiment, for example, as shown in FIGS. 4 (a) and 4 (b), a storage chamber 113 is required by one swelling suppressing portion 109a during use, especially during freezing and thawing. It is possible to suppress the swelling above. In particular, the effect of suppressing the deformation of the accommodation chamber 113 due to the port portion 115 can be expected. Moreover, one swelling suppressing portion 109a does not hinder the removal of the storage liquid 130 by the pipette 120 at the time of thawing.

なお、第3実施形態に係る細胞保存用バッグにおいては、1本の膨れ抑制部を破線状に複数に分散配置した構成としても良いし、ピペットの挿通方向によらず、複数の膨れ抑制部を配置する構成とすることもできる。 In the cell storage bag according to the third embodiment, one swelling suppressing portion may be dispersed and arranged in a plurality of broken lines, or the plurality of swelling suppressing portions may be arranged regardless of the insertion direction of the pipette. It can also be configured to be placed.

また、膨れ抑制部109aに代えて、第2実施形態として示した膨れ抑制部109bを適用することも可能である。 Further, instead of the swelling suppressing portion 109a, the swelling suppressing portion 109b shown as the second embodiment can be applied.

<第4実施形態>
図5は、本発明の第4実施形態に係る細胞保存用バッグ(使用前)の構成例を概略的に示す正面図である。なお、図5において、第1実施形態の細胞保存用バッグと同一部分には、同一または類似の符号を付し、詳しい説明は省略する。
<Fourth Embodiment>
FIG. 5 is a front view schematically showing a configuration example of a cell storage bag (before use) according to a fourth embodiment of the present invention. In FIG. 5, the same parts as the cell storage bag of the first embodiment are designated by the same or similar reference numerals, and detailed description thereof will be omitted.

即ち、本実施形態の細胞保存用バッグは、例えば図5に示すように、使用時、特に凍結時および解凍時に収容室113の形状が必要以上に変形しないように、容器本体100を補強するための膨れ抑制部109cが設けられている。この膨れ抑制部109cは、ピペット(図示省略)の挿通方向に沿って、第1のシート状部材と第2のシート状部材とが収容室113の中央部付近において、ほぼ線状に連続するようにして圧着されている。 That is, the cell storage bag of the present embodiment reinforces the container body 100 so that the shape of the storage chamber 113 is not unnecessarily deformed during use, especially during freezing and thawing, as shown in FIG. 5, for example. The swelling suppressing portion 109c is provided. In the swelling suppressing portion 109c, the first sheet-shaped member and the second sheet-shaped member are substantially linearly continuous in the vicinity of the central portion of the storage chamber 113 along the insertion direction of the pipette (not shown). It is crimped.

本実施形態において、膨れ抑制部109cは、収容室113内へのピペットの挿通の妨げとなるのを回避するために、剥離可能な構成、例えば、第1のシート状部材と第2のシート状部材とを溶着よりも脆弱な圧着によって接合した構成とされている。 In the present embodiment, the swelling suppressing portion 109c has a removable configuration, for example, a first sheet-like member and a second sheet-like structure, in order to avoid obstructing the insertion of the pipette into the storage chamber 113. The structure is such that the members are joined by crimping, which is weaker than welding.

例えば、凍結時には圧着状態とされて収容室113が膨らむのを抑制した膨れ抑制部109cは、ピペットの挿通に伴って剥離状態となることが可能とされている。 For example, the swelling suppressing portion 109c, which is in a crimped state at the time of freezing and suppresses the swelling of the storage chamber 113, can be in a peeled state with the insertion of the pipette.

その他の構成は、第1実施形態で示した細胞保存用バッグとほぼ同様である。 Other configurations are almost the same as the cell storage bag shown in the first embodiment.

本実施形態によれば、例えば図5に示すように、使用時、特に凍結時および解凍時においては、膨れ抑制部109cによって、収容室113が必要以上に膨らむのを抑制することが可能となる。しかも、安価であり、膨れ抑制部109cを剥離可能な構成としたことにより、解凍時にピペットによる保存液の取り出しの妨げとなることもない。 According to the present embodiment, for example, as shown in FIG. 5, during use, especially during freezing and thawing, the swelling suppressing portion 109c can suppress the storage chamber 113 from swelling more than necessary. .. Moreover, it is inexpensive and has a structure in which the swelling suppressing portion 109c can be peeled off, so that it does not interfere with the removal of the storage solution by the pipette at the time of thawing.

なお、膨れ抑制部109cは、例えば、着脱が繰り返し可能な線状の面ファスナーやチャック式またはマグネット式のジッパーなどでも良い。 The swelling suppressing portion 109c may be, for example, a linear hook-and-loop fastener or a chuck type or magnet type zipper that can be repeatedly attached and detached.

また、膨れ抑制部109cとしては、1本に限らず、複数の膨れ抑制部を備えた構成とすることも可能である(図示省略)。 Further, the swelling suppressing portion 109c is not limited to one, and may be configured to include a plurality of swelling suppressing portions (not shown).

<第5実施形態>
図6は、本発明の第5実施形態に係る細胞保存用バッグ(使用前)の構成例を概略的に示す正面図である。なお、図6において、第4実施形態の細胞保存用バッグと同一部分には、同一または類似の符号を付し、詳しい説明は省略する。
<Fifth Embodiment>
FIG. 6 is a front view schematically showing a configuration example of a cell storage bag (before use) according to the fifth embodiment of the present invention. In FIG. 6, the same parts as the cell storage bag of the fourth embodiment are designated by the same or similar reference numerals, and detailed description thereof will be omitted.

即ち、本実施形態の細胞保存用バッグは、例えば図6に示すように、使用時、特に凍結時および解凍時に収容室113の形状が必要以上に変形しないように補強するための膨れ抑制部109dが、破線状に複数に分散配置されている。 That is, as shown in FIG. 6, for example, the cell storage bag of the present embodiment has a swelling suppressing portion 109d for reinforcing the shape of the storage chamber 113 so as not to be unnecessarily deformed during use, particularly during freezing and thawing. However, they are distributed and arranged in a plurality of broken lines.

その他の構成は、第4実施形態で示した細胞保存用バッグとほぼ同様である。 Other configurations are substantially the same as the cell storage bag shown in the fourth embodiment.

本実施形態においては、膨れ抑制部109dを分散させて配置することによって、収容室113が膨らむのを容易に抑制しつつも、収容室113内へのピペット(図示省略)の挿通時にはより容易に剥離状態となり、保存液の取り出しの妨げとなるのを回避可能となる。 In the present embodiment, by arranging the swelling suppressing portions 109d in a dispersed manner, it is possible to easily suppress the swelling of the accommodation chamber 113, but it is easier to insert the pipette (not shown) into the accommodation chamber 113. It is possible to avoid being in a peeled state and hindering the removal of the storage solution.

<第6実施形態>
図7(a),図7(b)は、本発明の第6実施形態に係る細胞保存用バッグ(使用前)の構成例を概略的に示すものであって、図7(a)は正面図、図7(b)は図7(a)の7b-7b線に沿う断面図である。また、図8(a)~図8(c)は、図7(a),図7(b)に示す細胞保存用バッグの使用例を概略的に示すものであって、図8(a)は正面図、図8(b)は図8(a)の8b-8b線に沿う断面図、図8(c)は図8(a)の8c-8c線に沿う断面図である。なお、図7(a),図7(b)および図8(a)~図8(c)において、第5実施形態の細胞保存用バッグと同一部分には、同一または類似の符号を付し、詳しい説明は省略する。
<Sixth Embodiment>
7 (a) and 7 (b) show schematically a configuration example of the cell storage bag (before use) according to the sixth embodiment of the present invention, and FIG. 7 (a) shows the front surface. FIG. 7 (b) is a cross-sectional view taken along the line 7b-7b of FIG. 7 (a). 8 (a) to 8 (c) schematically show an example of using the cell storage bag shown in FIGS. 7 (a) and 7 (b), and FIG. 8 (a) shows. 8 (b) is a front view, FIG. 8 (b) is a cross-sectional view taken along line 8b-8b of FIG. 8 (a), and FIG. 8 (c) is a cross-sectional view taken along line 8c-8c of FIG. 8 (a). In FIGS. 7 (a), 7 (b) and 8 (a) to 8 (c), the same parts as the cell storage bag of the fifth embodiment are designated by the same or similar reference numerals. , Detailed explanation is omitted.

本実施形態の場合、例えば図7(a),図7(b)に示すように、後述するピペットの挿通方向に沿って、複数(ここでは、4本)の膨れ抑制部109eが、それぞれ、所定の距離を保ちつつ、互いに平行に、かつ、ほぼ破線状に複数に分割配置した構成とされている。 In the case of the present embodiment, for example, as shown in FIGS. 7 (a) and 7 (b), a plurality of (here, four) swelling suppressing portions 109e are provided along the insertion direction of the pipette described later, respectively. It is configured to be divided into a plurality of parts in parallel with each other and in a substantially broken line shape while maintaining a predetermined distance.

4本の膨れ抑制部109eは、例えば収容室113のほぼ中央部付近において、いずれも、第1のシート状部材101および第2のシート状部材103を圧着した構成とされている。 The four swelling suppressing portions 109e are configured such that the first sheet-shaped member 101 and the second sheet-shaped member 103 are crimped to each other, for example, in the vicinity of the substantially central portion of the accommodation chamber 113.

また、4本の膨れ抑制部109eは、所定の距離、例えば図8(a)~図8(c)に示すように、ポート部105からのピペット120の挿通が可能な距離よりも狭い間隔で配置されている。 Further, the four swelling suppressing portions 109e are provided at a predetermined distance, for example, at a distance narrower than the distance through which the pipette 120 can be inserted from the port portion 105, as shown in FIGS. 8 (a) to 8 (c). Have been placed.

本実施形態においては、ピペット120の太さ(径)よりも膨れ抑制部109eの間隔が狭いので、収容室113内へのピペット120の挿通に伴って、膨れ抑制部109eが容易に剥離状態となる。これにより、膨れ抑制部109eは、収容室113が膨らむのを抑制しつつも、保存液130の取り出しの妨げとなるのを容易に回避可能となる。 In the present embodiment, since the distance between the swelling suppressing portions 109e is narrower than the thickness (diameter) of the pipette 120, the swelling suppressing portion 109e is easily peeled off as the pipette 120 is inserted into the storage chamber 113. Become. As a result, the swelling suppressing portion 109e can easily avoid hindering the removal of the storage liquid 130 while suppressing the swelling of the storage chamber 113.

即ち、ポート部105から収容室113内へのピペット120の挿通に伴って、剥離状態となる程度の圧着により膨れ抑制部109eを形成するようにしたので、ピペット120の太さよりも狭い間隔で膨れ抑制部109eを配置することが可能となる。したがって、断面形状が変形し易い収容室113を、より多くの膨れ抑制部109eによって補強できるようになる。しかも、膨れ抑制部109eの間隔に応じて専用のピペットを新規に開発したりすることなく、既存のピペット120による保存液130の出し入れ、特に取り出しが容易に可能となる。 That is, as the pipette 120 is inserted from the port portion 105 into the accommodation chamber 113, the swelling suppressing portion 109e is formed by crimping to the extent that the pipette 120 is in a peeled state, so that the swelling suppressing portion 109e swells at intervals narrower than the thickness of the pipette 120. It becomes possible to arrange the suppression unit 109e. Therefore, the accommodation chamber 113 whose cross-sectional shape is easily deformed can be reinforced by more swelling suppressing portions 109e. Moreover, the storage liquid 130 can be easily taken in and out by the existing pipette 120 without newly developing a dedicated pipette according to the interval of the swelling suppressing portion 109e.

なお、生体由来のサンプルを含む保存液130の収容は、ポート部105に近いところから、膨れ抑制部109eを剥離しないようにして行われる。 The storage solution 130 containing the sample derived from a living body is stored so as not to peel off the swelling suppressing portion 109e from a place close to the port portion 105.

本実施形態によれば、例えば図8(a)~図8(c)に示すように、使用時、特に凍結時および解凍時においては、4本の膨れ抑制部109eによって、収容室113が必要以上に膨らむのを抑制することが可能となる。しかも、4本の膨れ抑制部109aが、解凍時にピペット120による保存液130の取り出しを妨げることもなく、ピペット120の先端部分が、収容室113の最深部の近傍にまで容易に到達可能とされる。よって、既存のピペット120による保存液130の出し入れが可能な、安価な細胞保存用バッグとすることができる。 According to the present embodiment, for example, as shown in FIGS. 8 (a) to 8 (c), a storage chamber 113 is required by the four swelling suppressing portions 109e during use, especially during freezing and thawing. It is possible to suppress the swelling above. Moreover, the four swelling suppressing portions 109a do not prevent the pipette 120 from taking out the storage solution 130 at the time of thawing, and the tip portion of the pipette 120 can easily reach the vicinity of the deepest portion of the storage chamber 113. Ru. Therefore, it is possible to provide an inexpensive cell storage bag that allows the storage liquid 130 to be taken in and out by the existing pipette 120.

なお、膨れ抑制部109eとしては、4本に限らず、また、膨れ抑制部が、ほぼ線状に連続するようにして配置された構成としても良い。 The swelling suppressing portion 109e is not limited to four, and the swelling suppressing portion may be arranged so as to be substantially linearly continuous.

<他の実施形態>
上記した各実施形態の細胞保存用バッグにおいては、いずれも、第1のシート状部材101と第2のシート状部材103とで形成される収容室113が、より十分な容量となるように構成することも可能である。
<Other embodiments>
In each of the cell storage bags of the above-described embodiments, the storage chamber 113 formed by the first sheet-shaped member 101 and the second sheet-shaped member 103 is configured to have a more sufficient capacity. It is also possible to do.

即ち、例えば図9(a)に示すように、収容室113が十分な容量となるように、第1のシート状部材101に余裕を持たせた状態で、膨れ抑制部109を形成するようにしても良い。 That is, for example, as shown in FIG. 9A, the swelling suppressing portion 109 is formed with a margin in the first sheet-shaped member 101 so that the accommodation chamber 113 has a sufficient capacity. May be.

ここで、膨れ抑制部109は、上述の膨れ抑制部109a、109b、109c、109d、109eを総称したものとなっている。 Here, the swelling suppressing portion 109 is a general term for the above-mentioned swelling suppressing portions 109a, 109b, 109c, 109d, 109e.

なお、例えば図9(b)に示すように、収容室113が十分な容量となるように、第2のシート状部材103に余裕を持たせた状態で、膨れ抑制部109を形成するようにしても良い。 In addition, for example, as shown in FIG. 9B, the swelling suppressing portion 109 is formed with a margin in the second sheet-shaped member 103 so that the accommodation chamber 113 has a sufficient capacity. May be.

または、例えば図9(c)に示すように、収容室113が十分な容量となるように、第1のシート状部材101および第2のシート状部材103に余裕を持たせた状態で、膨れ抑制部109を形成するようにしても良い。 Alternatively, as shown in FIG. 9 (c), for example, the first sheet-shaped member 101 and the second sheet-shaped member 103 are swollen with a margin so that the storage chamber 113 has a sufficient capacity. The restraining portion 109 may be formed.

上述したように、本発明の各実施形態に係る細胞保存用バッグによれば、いずれの場合にも、凍結時および解凍時に、細胞保存用バッグの収容室113が必要以上に膨らむのを抑制できるようになる。 As described above, according to the cell storage bag according to each embodiment of the present invention, in any case, it is possible to prevent the storage chamber 113 of the cell storage bag from swelling more than necessary during freezing and thawing. Will be.

即ち、第1のシート状部材101と第2のシート状部材103とからなる容器本体100の、生体由来のサンプルを含む保存液130を収容する収容室113に、膨れ抑制部109(109a,109b,109c,109d,109e)を備えるようにしている。これにより、第1のシート状部材101および第2のシート状部材103のシート厚をより薄くした場合にも、凍結時および解凍時に収容室113が膨張し、形状が変形するのを抑制できるようになる。その結果、第1のシート状部材101および第2のシート状部材103のシート厚をできる限り薄くすることが可能となる。したがって、細胞を傷付けたり、細胞保存用バッグを壊したりすることなしに、保存液130に対する熱の伝わりを良くすることができ、保存液130の凍結時間や解凍時間を大幅に短縮できるようになるものである。 That is, the swelling suppressing portion 109 (109a, 109b) is placed in the storage chamber 113 of the container body 100 including the first sheet-shaped member 101 and the second sheet-shaped member 103, which houses the storage liquid 130 containing the sample derived from a living body. , 109c, 109d, 109e). As a result, even when the sheet thickness of the first sheet-shaped member 101 and the second sheet-shaped member 103 is made thinner, the storage chamber 113 can be prevented from expanding and deforming during freezing and thawing. become. As a result, the sheet thickness of the first sheet-shaped member 101 and the second sheet-shaped member 103 can be made as thin as possible. Therefore, heat transfer to the storage solution 130 can be improved without damaging the cells or breaking the cell storage bag, and the freezing time and thawing time of the storage solution 130 can be significantly shortened. It is a thing.

なお、各実施形態においては、細胞保存用バッグとして、いずれもスクリュー式のキャップ107付きのポート部105、115を備えた構成を例示したが、これに限らず、シールなどを用いて密閉するものであっても良い。 In each embodiment, as the cell storage bag, a configuration including port portions 105 and 115 with a screw-type cap 107 is exemplified, but the present invention is not limited to this, and the cell is sealed by using a seal or the like. It may be.

以上、実施の形態を例示して本発明の一態様について説明したが、一例であり、特許請求の範囲に記載される発明の範囲は、発明の要旨を逸脱しない範囲内で種々変更できるものである。 Although one aspect of the present invention has been described above by exemplifying an embodiment, it is an example, and the scope of the invention described in the claims can be variously changed within a range that does not deviate from the gist of the invention. be.

100 容器本体(袋体)
101 第1のシート状部材
103 第2のシート状部材
105、115 ポート部
107 スクリュー式のキャップ(蓋部)
109、109a、109b、109c、109d、109e 膨れ抑制部(膨張抑制部)
111 周縁溶着部
113 収容室
120 ピペット
130 保存液(生体由来のサンプルを含む液体)
100 Container body (bag body)
101 First sheet-like member 103 Second sheet-like member 105, 115 Port portion 107 Screw-type cap (cover portion)
109, 109a, 109b, 109c, 109d, 109e Swelling suppression unit (expansion suppression unit)
111 Peripheral welding part 113 Containment chamber 120 Pipette 130 Preservative solution (liquid containing biological sample)

Claims (5)

第1のシート状部材の周縁部と第2のシート状部材の周縁部とが溶着されて、該周縁部を除く部分に、生体由来のサンプルを含む液体を収容可能な収容室が形成された袋体を備え、前記袋体内へのピペットの挿通が可能な凍結保存容器であって、
前記袋体の内面には、前記第1のシート状部材と前記第2のシート状部材との間が前記溶着よりも脆弱に圧着されてなり、前記液体の凍結時および解凍時に、前記収容室の形状が過度に変形するのを抑制する膨張抑制部が、前記ピペットの挿通に伴って分離可能に設けられていることを特徴とする凍結保存容器。
The peripheral edge of the first sheet-shaped member and the peripheral edge of the second sheet-shaped member were welded to each other, and a storage chamber capable of containing a liquid containing a biological sample was formed in a portion other than the peripheral edge. A cryopreservation container equipped with a bag and capable of inserting a pipette into the bag.
The inner surface of the bag is crimped between the first sheet-shaped member and the second sheet-shaped member more fragilely than the welding, and the storage chamber is formed when the liquid is frozen and thawed. A cryopreservation container characterized in that an expansion suppressing portion for suppressing excessive deformation of the shape of the pipette is provided so as to be separable with the insertion of the pipette.
第1のシート状部材の周縁部と第2のシート状部材の周縁部とが溶着されて、該周縁部を除く部分に、生体由来のサンプルを含む液体を収容可能な収容室が形成された袋体を備え、前記袋体内へのピペットの挿通が可能な凍結保存容器であって、
前記袋体の内面には、前記第1のシート状部材と前記第2のシート状部材との間が前記溶着よりも脆弱に圧着されてなり、前記液体の凍結時および解凍時に、前記収容室の形状が過度に変形するのを抑制する膨張抑制部が、前記ピペットの挿通位置に対応する前記収容室の中央部分に線状に連続配置されるとともに、前記ピペットの挿通に伴って分離可能に設けられていることを特徴とする凍結保存容器
The peripheral edge of the first sheet-shaped member and the peripheral edge of the second sheet-shaped member were welded to each other, and a storage chamber capable of containing a liquid containing a biological sample was formed in a portion other than the peripheral edge. A cryopreservation container equipped with a bag and capable of inserting a pipette into the bag.
On the inner surface of the bag, the space between the first sheet-shaped member and the second sheet-shaped member is crimped more fragilely than the welding, and the storage chamber is formed when the liquid is frozen and thawed. An expansion suppressing portion that suppresses excessive deformation of the shape of the pipette is linearly and continuously arranged in the central portion of the storage chamber corresponding to the insertion position of the pipette, and can be separated with the insertion of the pipette. A cryopreservation container characterized by being provided .
第1のシート状部材の周縁部と第2のシート状部材の周縁部とが溶着されて、該周縁部を除く部分に、生体由来のサンプルを含む液体を収容可能な収容室が形成された袋体を備え、前記袋体内へのピペットの挿通が可能な凍結保存容器であって、
前記袋体の内面には、前記第1のシート状部材と前記第2のシート状部材との間が前記溶着よりも脆弱に圧着されてなり、前記液体の凍結時および解凍時に、前記収容室の形状が過度に変形するのを抑制する膨張抑制部が、前記ピペットの挿通位置に対応する前記収容室の中央部分に破線状に分散配置されるとともに、前記ピペットの挿通に伴って分離可能に設けられていることを特徴とする凍結保存容器
The peripheral edge of the first sheet-shaped member and the peripheral edge of the second sheet-shaped member were welded to each other, and a storage chamber capable of containing a liquid containing a biological sample was formed in a portion other than the peripheral edge. A cryopreservation container equipped with a bag and capable of inserting a pipette into the bag.
On the inner surface of the bag body, the space between the first sheet-shaped member and the second sheet-shaped member is crimped more fragilely than the welding, and the storage chamber is formed when the liquid is frozen and thawed. The expansion suppressing portion that suppresses excessive deformation of the shape of the pipette is distributed in a broken line shape in the central portion of the storage chamber corresponding to the insertion position of the pipette, and can be separated with the insertion of the pipette. A cryopreservation container characterized by being provided .
第1のシート状部材の周縁部と第2のシート状部材の周縁部とが溶着されて、該周縁部を除く部分に、生体由来のサンプルを含む液体を収容可能な収容室が形成された袋体を備え、前記袋体内へのピペットの挿通が可能な凍結保存容器であって、
前記袋体の内面には、前記第1のシート状部材と前記第2のシート状部材との間が前記溶着よりも脆弱に圧着されてなり、前記液体の凍結時および解凍時に、前記収容室の形状が過度に変形するのを抑制する膨張抑制部が、前記ピペットの挿通が部分的に可能な距離だけ離間して複数配置されるとともに、前記ピペットの挿通に伴って分離可能に設けられていることを特徴とする凍結保存容器
The peripheral edge of the first sheet-shaped member and the peripheral edge of the second sheet-shaped member were welded to each other, and a storage chamber capable of containing a liquid containing a biological sample was formed in a portion other than the peripheral edge. A cryopreservation container equipped with a bag and capable of inserting a pipette into the bag.
On the inner surface of the bag, the space between the first sheet-shaped member and the second sheet-shaped member is crimped more fragilely than the welding, and the storage chamber is formed when the liquid is frozen and thawed. A plurality of expansion suppressing portions for suppressing excessive deformation of the shape of the pipette are arranged at a distance that allows the pipette to be partially inserted , and are separately provided along with the insertion of the pipette. A cryopreservation container characterized by being present .
前記ピペットを挿通させるために、前記収容室の少なくとも一部に連通するように、前記第1のシート状部材の周縁部と前記第2のシート状部材の周縁部とに溶着されるとともに、開閉可能な蓋部を有したポート部を、さらに備えることを特徴とする請求項1から4のいずれか1項に記載の凍結保存容器。 In order to insert the pipette, it is welded to the peripheral edge of the first sheet-shaped member and the peripheral edge of the second sheet-shaped member so as to communicate with at least a part of the storage chamber, and is opened and closed. The cryopreservation container according to any one of claims 1 to 4 , further comprising a port portion having a possible lid portion.
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WO2017026131A1 (en) 2015-08-11 2017-02-16 上田製袋株式会社 Cryopreservation bag, and method for enclosing biological tissue in cryopreservation bag
JP2017217195A (en) 2016-06-07 2017-12-14 大日本印刷株式会社 Freezing multi-chamber container

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WO2017026131A1 (en) 2015-08-11 2017-02-16 上田製袋株式会社 Cryopreservation bag, and method for enclosing biological tissue in cryopreservation bag
JP2017217195A (en) 2016-06-07 2017-12-14 大日本印刷株式会社 Freezing multi-chamber container

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